Cytochrome p450 oxidase sto1, encoding gene and application thereof
By identifying and cloning the cytochrome P450 oxidase STO1 from potato, and constructing an engineered strain of Saccharomyces cerevisiae, the problem of the lack of identification of the key enzyme in the synthesis of spirotropin was solved, and the efficient synthesis of spirotropin was achieved, laying the foundation for industrial production.
Patent Information
- Application Number
- CN202511575782.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-31
- Publication Date
- 2026-06-19
- Estimated Expiration
- 2045-10-31
AI Technical Summary
The cytochrome P450 oxidase responsible for catalyzing the synthesis of spirotropin has not yet been cloned and identified from potatoes, limiting in-depth research on the biosynthetic mechanism of spirotropin and its industrial production.
Cytochrome P450 oxidase STO1 was identified and cloned from potatoes. By constructing an engineered strain of Saccharomyces cerevisiae, genetic modification and heterologous expression technologies were used to catalyze the conversion of tofu zearalenone into spirophyllotoxin and/or spirophyllotoxin ketone. This included replacing the ERG9 genome with the weak promoter HXT1, expressing cytochrome P450 oxidase STO1, cytochrome P450 reductase AtCPR, and terpene synthase STS.
The efficient heterologous synthesis of spirochetone was achieved, with a laboratory shake flask yield of 291 mg/L, providing a theoretical basis for industrial production and enriching the diversity of cytochrome P450 oxidases.