A maize mitogen-activated protein kinase kinase gene ZmMKK1 and application thereof

By cloning and overexpressing the maize mitogen-activated protein kinase kinase gene ZmMKK1, the problem of unclear maize response mechanism to high temperature stress was solved, and the heat resistance of the plant was improved. This has important theoretical guiding significance and agricultural application value.

CN117965577BActive Publication Date: 2026-05-29ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ANHUI AGRICULTURAL UNIVERSITY
Filing Date
2024-02-01
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Current technologies do not provide in-depth research on the physiological and molecular mechanisms of maize's response to high-temperature stress, which affects maize yield. Improving maize's high-temperature tolerance through biological means is an important research topic.

Method used

The maize mitogen-activated protein kinase kinase gene ZmMKK1 was cloned and overexpressed in plants. Gene editing was performed using recombinant plasmids and primer pairs to construct overexpression and knockout mutants, and their functions were identified.

Benefits of technology

Under high temperature stress, plants that overexpress the ZmMKK1 gene have a higher survival rate, while plants that knock out the ZmMKK1 gene have a lower heat tolerance. This indicates that the ZmMKK1 gene plays a positive regulatory role in the heat tolerance of plants and helps to improve the plant's ability to resist high temperature stress.

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Abstract

The application discloses a corn mitogen-activated protein kinase kinase gene ZmMKK1 and application thereof, relates to the technical field of plant genetic engineering, and the nucleotide sequence of the gene ZmMKK1 is shown as SEQ ID NO. 1. The ZmMKK1 gene is transferred into Arabidopsis thaliana by a method of agrobacterium mediation, and the result shows that the heat resistance of the transgenic Arabidopsis thaliana is obviously improved by overexpressing the ZmMKK1 gene. The ZmMKK1 knockout corn mutant is subjected to heat treatment, and it is found that the heat resistance of the ZmMKK1 knockout corn mutant is obviously reduced, which indicates that the ZmMKK1 positively regulates the heat resistance of plants. The application will be helpful to improving the high-temperature stress resistance of plants, has important theoretical guiding significance for cultivating new crop varieties resistant to high temperature, and has great application value for agricultural production in China.
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