Application of pyridine-2, 6-dicarboxylic acid aqueous solution in inhibition of plant soil-borne diseases

Abstract

The invention relates to the technical field of plant disease control, in particular to application of a pyridine-2, 6-dicarboxylic acid aqueous solution in inhibition of plant soil-borne diseases. According to the application, the pyridine-2, 6-dicarboxylic acid aqueous solution is applied to inhibition of paulownia soil-borne diseases for the first time, and the pyridine-2, 6-dicarboxylic acid is applied to paulownia continuous cropping soil, so that growth of fusarium key soil-borne pathogenic bacteria can be efficiently and specifically inhibited. The compound has a significant dose-dependent inhibition effect on fusarium, and effectively blocks the main pathogenic pathway of paulownia replanting disease. A healthy root growth environment is created for paulownia. Pot experiments prove that the biomass of the treated paulownia plants is obviously increased, and the root development condition is obviously improved.

Description

Technical Field

[0001] This invention relates to the field of plant disease control technology, specifically to the application of pyridine-2,6-dicarboxylic acid aqueous solution in inhibiting soil-borne plant diseases. Background Technology

[0002] Paulownia ( Paulownia Paulownia (Paulownia spp.) is characterized by rapid growth, excellent timber quality, and high economic value, playing an important role in forestry production and ecological construction. However, with the continuous expansion of Paulownia plantations and the increasing prevalence of continuous cropping, the resulting replanting disease problem is becoming increasingly serious. Replanting disease mainly manifests as stunted growth, significantly reduced survival rate, and a sharp decline in biomass, severely restricting the sustainable development of the Paulownia industry.

[0003] Fusarium ( Fusarium Fusarium spp. is the key pathogen causing replanting disease in Paulownia. This type of pathogen can survive in the soil for a long time, infecting the Paulownia root system, causing root rot, and disrupting the plant's nutrient absorption and water transport systems, thus severely affecting its normal growth and development. Studies have shown that the large-scale accumulation of Fusarium pathogens in continuously cropped soils is one of the main reasons for the decline in Paulownia growth. Currently, the treatment of Paulownia replanting disease mainly relies on agricultural measures (such as crop rotation). However, crop rotation is difficult to implement in large-scale forest land, has poor economic efficiency, and cannot meet the needs of modern forestry production.

[0004] Therefore, there is an urgent need to develop a new strategy that can suppress soil-borne diseases of paulownia caused by Fusarium to promote the healthy growth of paulownia. Summary of the Invention

[0005] To address the above problems, this invention provides the application of pyridine-2,6-dicarboxylic acid aqueous solution in inhibiting soil-borne plant diseases.

[0006] Application of pyridine-2,6-dicarboxylic acid aqueous solution in inhibiting soil-borne plant diseases, wherein the concentration of the pyridine-2,6-dicarboxylic acid aqueous solution is 50 mg / L to 500 mg / L.

[0007] Preferably, the plant is Paulownia.

[0008] Preferably, the soil-borne disease is paulownia replant disease caused by Fusarium.

[0009] Preferably, the aqueous solution of pyridine-2,6-dicarboxylic acid is prepared by dissolving pyridine-2,6-dicarboxylic acid in water.

[0010] Preferably, the water is sterile water.

[0011] Preferably, the pyridine-2,6-dicarboxylic acid aqueous solution is applied by irrigation into the soil where the paulownia tree is planted.

[0012] Preferably, the application amount of the pyridine-2,6-dicarboxylic acid aqueous solution is 6L / m 2 60L / m 2 Soil.

[0013] Preferably, the application time of the pyridine-2,6-dicarboxylic acid aqueous solution is before planting or during the growth of Paulownia.

[0014] Compared with the prior art, the present application has the following beneficial effects: The present application provides a pyridine-2,6-dicarboxylic acid aqueous solution for use in inhibiting soil-borne diseases of plants, the concentration of the pyridine-2,6-dicarboxylic acid aqueous solution being 50mg / L-500mg / L. The present application first applies the pyridine-2,6-dicarboxylic acid aqueous solution to inhibit soil-borne diseases of Paulownia, and applies the pyridine-2,6-dicarboxylic acid to the continuous cropping soil of Paulownia, which can efficiently and specifically inhibit the growth of the key soil-borne pathogenic fungus Fusarium. The examples show that the ingredient has a significant dose-dependent inhibitory effect on Fusarium, and at a concentration of 500mg / L, the abundance of Fusarium in the soil can be reduced by as much as 80%, effectively blocking the main pathogenic pathway of Paulownia replant disease. By effectively controlling soil-borne diseases, the present application creates a healthy root growth environment for Paulownia. Pot experiment confirms that the biomass of the treated Paulownia plants is significantly increased, and the root development condition is obviously improved, solving the core problems of low survival rate and growth inhibition caused by continuous cropping. In addition, the application of the pyridine-2,6-dicarboxylic acid aqueous solution effectively improves the soil microbial community structure and improves the soil health degree; compared with chemical fumigants, pyridine-2,6-dicarboxylic acid as a naturally occurring compound is easily degradable in the environment and has no harmful residues, avoiding the damage of traditional chemical agents to the soil ecosystem, and meeting the requirements of green forestry development. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor.

[0016] Figure 1 Figure for the inhibitory effect of pyridine-2,6-dicarboxylic acid of the present application on Fusarium.

[0017] Figure 2 Figure for the influence of pyridine-2,6-dicarboxylic acid of the present application on the growth of Paulownia in continuous cropping soil.

[0018] Figure 3Figure of the effect of pyridine-2,6-dicarboxylic acid on the biomass of Paulownia in continuous cropping soil according to the present application.

[0019] Figure 4 Figure of the effect of pyridine-2,6-dicarboxylic acid on the abundance of Fusarium in continuous cropping soil according to the present application. DETAILED DESCRIPTION

[0020] In order to facilitate the understanding of the present application, the present application will be described more fully below and preferred embodiments of the present application will be given. However, the present application can be realized in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided so that the disclosure of the present application can be more thoroughly and completely understood.

[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terminology used in the description of the present application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application.

[0022] The beneficial effects of the present application are illustrated below through specific examples.

[0023] Pyridine-2,6-dicarboxylic acid was purchased from Shanghai McLean Biochemical Science and Technology Co., Ltd., item number: P816098.

[0024] The Fusarium used in the present application is Fusarium solani Fusarium solani , which is isolated from continuous cropping soil of Paulownia and purchased from Zhengzhou Fangjue Biological Technology Co., Ltd.

[0025] PDA medium is a common commercially available medium.

[0026] Example 1 PDA medium with pyridine-2,6-dicarboxylic acid content of 0.5 mg / L was configured as the experimental group, and PDA medium without pyridine-2,6-dicarboxylic acid was configured as the control group (CK). Equal amounts of Fusarium were inoculated in the experimental group and the control group.

[0027] The results are shown in Figure 1 , it was found that pyridine-2,6-dicarboxylic acid can significantly inhibit the growth of Fusarium after 3 days, and the inhibitory effect is more obvious after 6 days.

[0028] Example 2 PDA medium with pyridine-2,6-dicarboxylic acid content of 1 mg / L was configured as the experimental group, and PDA medium without pyridine-2,6-dicarboxylic acid was configured as the control group (CK). Equal amounts of Fusarium were inoculated in the experimental group and the control group.

[0029] The results are shown in Figure 1As shown in the figure, it was found that pyridine-2,6-dicarboxylic acid could significantly inhibit the growth of Fusarium after 3d, and the inhibitory effect was more obvious after 6d.

[0030] Example 3 Pyridine-2,6-dicarboxylic acid was weighed, and sterile water was used as the solvent to prepare pyridine-2,6-dicarboxylic acid aqueous solutions with concentrations of 50 mg / L and 500 mg / L, respectively.

[0031] The pot experiment was designed as follows: The soil infected with Fusarium was treated with pyridine-2,6-dicarboxylic acid aqueous solutions with concentrations of 50 mg / L and 500 mg / L, respectively, and the inoculation amount of Fusarium in the soil was 1×10 4 CFU of Fusarium spores per gram of dry soil. Five milliliters were used for each pot, and the treatment was continuously applied for three days, while the control group was irrigated with the same amount of sterile water. After two weeks of treatment, the plant growth indicators were measured. The results showed that compared with the control group, the plant height, root length and fresh weight of the Paulownia plant in the 50 mg / L and 500 mg / L pyridine-2,6-dicarboxylic acid aqueous solution treatment groups were significantly improved, and the plant biomass increased by 96.63% and 350%, respectively. Further analysis of the abundance of Fusarium in the rhizosphere soil using absolute quantitative high-throughput sequencing technology showed that the number of Fusarium in the rhizosphere soil of the treatment groups treated with 50 mg / L and 500 mg / L pyridine-2,6-dicarboxylic acid aqueous solution decreased by 20% and 80% respectively compared with the control. The results are shown in Figures 2-4 .

[0032] Example 4 A soil treatment method for inhibiting soil-borne diseases and promoting the growth of Paulownia, specifically comprising the following steps: (1) Prepare a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 50 mg / L.

[0033] (2) Before planting the Paulownia, uniformly irrigate the above-mentioned pyridine-2,6-dicarboxylic acid aqueous solution into the soil, and the application amount is 6 liters of pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0034] Example 5 A soil treatment method for inhibiting soil-borne diseases and promoting the growth of Paulownia, specifically comprising the following steps: (1) Prepare a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 50 mg / L.

[0035] (2) Before planting the Paulownia, uniformly irrigate the above-mentioned pyridine-2,6-dicarboxylic acid aqueous solution into the soil, and the application amount is 30 liters of pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0036] Example 6 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) preparing a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 50 mg / L.

[0037] (2) before planting the Paulownia, evenly irrigating the pyridine-2,6-dicarboxylic acid aqueous solution into the soil, with a use amount of 60 liters of the pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0038] Example 7 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) preparing a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 50 mg / L.

[0039] (2) during the growth period of the Paulownia, evenly irrigating the pyridine-2,6-dicarboxylic acid aqueous solution into the soil, with a use amount of 6 liters of the pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0040] Example 8 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) preparing a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 50 mg / L.

[0041] (2) during the growth period of the Paulownia, evenly irrigating the pyridine-2,6-dicarboxylic acid aqueous solution into the soil, with a use amount of 30 liters of the pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0042] Example 9 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) preparing a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 50 mg / L.

[0043] (2) during the growth period of the Paulownia, evenly irrigating the pyridine-2,6-dicarboxylic acid aqueous solution into the soil, with a use amount of 60 liters of the pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0044] Example 10 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) preparing a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 300 mg / L.

[0045] (2) before planting the Paulownia, evenly irrigating the pyridine-2,6-dicarboxylic acid aqueous solution into the soil, with a use amount of 6 liters of the pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0046] Example 11 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) Prepare a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 300 mg / L.

[0047] (2) Before planting Paulownia, evenly irrigate the above pyridine-2,6-dicarboxylic acid aqueous solution into the soil, and the application amount is 30 liters of pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0048] Example 12 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) Prepare a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 300 mg / L.

[0049] (2) Before planting Paulownia, evenly irrigate the above pyridine-2,6-dicarboxylic acid aqueous solution into the soil, and the application amount is 60 liters of pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0050] Example 13 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) Prepare a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 300 mg / L.

[0051] (2) During the growth period of Paulownia, evenly irrigate the above pyridine-2,6-dicarboxylic acid aqueous solution into the soil, and the application amount is 6 liters of pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0052] Example 14 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) Prepare a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 300 mg / L.

[0053] (2) During the growth period of Paulownia, evenly irrigate the above pyridine-2,6-dicarboxylic acid aqueous solution into the soil, and the application amount is 30 liters of pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0054] Example 15 A soil treatment method for inhibiting soil-borne diseases and promoting growth of Paulownia, comprising the following steps: (1) Prepare a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 300 mg / L.

[0055] (2) In the growing period of Paulownia, the above pyridine-2, 6-dicarboxylic acid aqueous solution is evenly irrigated into the soil, and the application amount is 60 liters of pyridine-2, 6-dicarboxylic acid aqueous solution per square meter of soil.

[0056] Example 16 A soil treatment method for inhibiting soil-borne diseases and promoting the growth of Paulownia, specifically comprising the following steps: (1) Prepare a pyridine-2, 6-dicarboxylic acid aqueous solution with a concentration of 500 mg / L.

[0057] (2) Before planting Paulownia, the above pyridine-2, 6-dicarboxylic acid aqueous solution is evenly irrigated into the soil, and the application amount is 6 liters of pyridine-2, 6-dicarboxylic acid aqueous solution per square meter of soil.

[0058] Example 17 A soil treatment method for inhibiting soil-borne diseases and promoting the growth of Paulownia, specifically comprising the following steps: (1) Prepare a pyridine-2, 6-dicarboxylic acid aqueous solution with a concentration of 500 mg / L.

[0059] (2) In the growing period of Paulownia, the above pyridine-2, 6-dicarboxylic acid aqueous solution is evenly irrigated into the soil, and the application amount is 30 liters of pyridine-2, 6-dicarboxylic acid aqueous solution per square meter of soil.

[0060] Example 18 A soil treatment method for inhibiting soil-borne diseases and promoting the growth of Paulownia, specifically comprising the following steps: (1) Prepare a pyridine-2, 6-dicarboxylic acid aqueous solution with a concentration of 500 mg / L.

[0061] (2) Before planting Paulownia, the above pyridine-2, 6-dicarboxylic acid aqueous solution is evenly irrigated into the soil, and the application amount is 60 liters of pyridine-2, 6-dicarboxylic acid aqueous solution per square meter of soil.

[0062] Example 19 A soil treatment method for inhibiting soil-borne diseases and promoting the growth of Paulownia, specifically comprising the following steps: (1) Prepare a pyridine-2, 6-dicarboxylic acid aqueous solution with a concentration of 500 mg / L.

[0063] (2) In the growing period of Paulownia, the above pyridine-2, 6-dicarboxylic acid aqueous solution is evenly irrigated into the soil, and the application amount is 6 liters of pyridine-2, 6-dicarboxylic acid aqueous solution per square meter of soil.

[0064] Example 20 A soil treatment method for inhibiting soil-borne diseases and promoting the growth of Paulownia, specifically comprising the following steps: (1) prepare a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 500 mg / L.

[0065] (2) during the growth period of Paulownia, evenly irrigate the above pyridine-2,6-dicarboxylic acid aqueous solution into the soil, and the application amount is 30 liters of pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0066] Example 21 A soil treatment method for inhibiting soil-borne diseases and promoting the growth of Paulownia, specifically comprising the following steps: (1) prepare a pyridine-2,6-dicarboxylic acid aqueous solution with a concentration of 500 mg / L.

[0067] (2) during the growth period of Paulownia, evenly irrigate the above pyridine-2,6-dicarboxylic acid aqueous solution into the soil, and the application amount is 30 liters of pyridine-2,6-dicarboxylic acid aqueous solution per square meter of soil.

[0068] The technical features of the above-mentioned embodiments can be combined arbitrarily, and in order to make the description simple, all possible combinations of the technical features in the above-mentioned embodiments are not described, however, as long as the combination of the technical features does not exist contradictory, it should be considered as the scope of the present application.

[0069] The above-mentioned embodiments only express several embodiments of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent. For ordinary skilled in the art, without departing from the concept of the present application, a number of variations and improvements can be made, which are all within the scope of the present application. Therefore, the protection scope of the present application patent should be subject to the appended claims.

Claims

1. The application of pyridine-2,6-dicarboxylic acid aqueous solution in inhibiting soil-borne plant diseases, characterized in that, The concentration of the pyridine-2,6-dicarboxylic acid aqueous solution is 50 mg / L to 500 mg / L.

2. The application according to claim 1, characterized in that, The plant in question is Paulownia tomentosa.

3. The application according to claim 1, characterized in that, The soil-borne disease mentioned is paulownia replant disease caused by Fusarium.

4. The application according to claim 1, characterized in that, The aqueous solution of pyridine-2,6-dicarboxylic acid is prepared by dissolving pyridine-2,6-dicarboxylic acid in water.

5. The application according to claim 4, characterized in that, The water is sterile.

6. The application according to claim 1, characterized in that, The pyridine-2,6-dicarboxylic acid aqueous solution is applied by irrigation into the soil where paulownia trees are planted.

7. The application according to claim 6, characterized in that, The dosage of the pyridine-2,6-dicarboxylic acid aqueous solution is 6 L / m³. 2 ~60L / m 2 soil.

8. The application according to claim 6, characterized in that, The pyridine-2,6-dicarboxylic acid aqueous solution is applied before or during the growth period of the paulownia tree.

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