Herpetospermum seed core-satellite nanostructure composition as well as preparation method and application thereof

Abstract

The invention discloses a herpetospermum seed-satellite nanostructure composition as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. According to the herpetospermum seed core-satellite nanostructure composition, herpetospermum seeds are used as raw materials, and the uniform and stable herpetospermum seed core-satellite nanostructure composition is formed by adopting a micro-precipitation technology and then adopting a Top-Down technology. The application refers to the application in preparation of drugs for treating and / or preventing autoimmune hepatitis. The preparation method has the advantages that particle size distribution is uniform, and a system is stable; the average particle size is 200-300 nm, the PDI is 0.05-0.30, the absolute value of the potential is 40-60 mV, and TEM shows that the form is a core-satellite structure; the NLRP3 / Caspase-1 / IL-1beta pathway can be regulated and controlled, so that the inflammatory response can be alleviated, and the liver injury can be improved. The NLRP3 / Caspase-1 / IL-1beta pathway can be regulated and controlled.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a wavy melon seed kernel-satellite nanostructure composition, its preparation method and application, and particularly to a wavy melon seed kernel-satellite nanostructure composition for the prevention and treatment of autoimmune hepatitis, its preparation method and application. Background Technology

[0002] Immune-mediated liver injury is a disease of hepatocellular damage mediated by abnormal immune responses. It is characterized by inflammatory cell infiltration, degeneration, and inflammatory necrosis in liver tissue, ultimately leading to liver fibrosis, cirrhosis, and even liver cancer. Current clinical treatment mainly relies on glucocorticoids and immunosuppressants, but long-term use easily causes serious side effects and drug resistance, necessitating the development of novel, highly effective, and low-toxicity treatment strategies.

[0003] As a core ingredient in Tibetan medicine for treating liver diseases, *Pleurotus ostreatus* seeds are rich in various active components, including lignans and fatty oils, which exhibit synergistic effects across multiple targets. Studies have confirmed that *Pleurotus ostreatus* seed extract demonstrates a clear therapeutic advantage in an immune-mediated liver injury model. Among these, lignans, in particular, exhibit significant anti-inflammatory and hepatoprotective activities, while the fatty oils also show significant hepatoprotective effects.

[0004] For a long time, the clinical use of sunflower seeds has been severely limited due to the poor solubility and low permeability of many of their active ingredients. Studies have found that self-assembled nanoparticles formed during the decoction process of sunflower seeds can effectively improve the drug delivery performance of active ingredients, enhance absorption, and reduce adverse reactions. However, traditional water decoction methods suffer from low extraction rates of active ingredients and unstable self-assembled structures. While upgrades based on micro-precipitation technology have improved the transfer rate, micro-precipitated nanoparticles still suffer from problems affecting system stability, such as unstable particle size and fatty acid floating. Summary of the Invention

[0005] To address the problems existing in the prior art, the first objective of this invention is to disclose a wave-shaped sunflower seed kernel-satellite nanostructure composition; this wave-shaped sunflower seed kernel-satellite nanostructure composition is based on "micro-precipitation" and "Top-Down" technology, with lignans and fatty oils as the main components.

[0006] The second objective of this invention is to disclose a method for preparing the above-mentioned wave-shaped melon seed core-satellite nanostructure composition; this preparation method is based on "micro-precipitation" and "Top-Down" techniques.

[0007] The third objective of this invention is to disclose the application of the above-mentioned core-satellite nanostructure composition of wavy melon seeds; specifically, it discloses the application of the above-mentioned core-satellite nanostructure composition in drugs for the prevention and treatment of autoimmune hepatitis, especially its good therapeutic effect on Con A-induced autoimmune hepatitis.

[0008] The objective of this invention is achieved through the following technical solution: A wavy melon seed kernel-satellite nanostructure composition, wherein: the wavy melon seed kernel-satellite nanostructure composition uses wavy melon seeds as raw material, and first adopts micro-precipitation technology and then top-down technology to form a uniform and stable wavy melon seed kernel-satellite nanostructure composition; wherein the micro-precipitation technology refers to adding an appropriate concentration of traditional Chinese medicine alcohol extract solution to the water decoction; wherein the top-down technology refers to uniformly dispersing the upper fatty acids through high pressure homogenization technology.

[0009] A method for preparing a wave-shaped melon seed core-satellite nanostructure composition, wherein the preparation method includes the following steps: (1) Weigh out the wavy melon seeds; (2) Soak (1) in 12 times the amount of water for 30 min, reflux and decoct for 1 h, filter, and keep the dregs for later use; (3) Take the remaining residue of the decoction of the pine melon seeds in step (2), add 8 times the amount of 75% ethanol, extract for 1-2 hours, filter, and set aside. (4) Slowly add the alcohol extract of the pine nut seed residue prepared in step (3) to the decoction of the pine nut seed prepared in step (2), at 800 r·min -1 Stir at a certain speed for 5 minutes to obtain the product; (5) Take the product from step (4), remove the ethanol by rotary evaporation under reduced pressure at 45°C, and concentrate it to a concentration of 0.3 g crude drug·mL. -1 ; (6) Take the product from step (5), place it in a high-pressure homogenizer, and homogenize it for 10 cycles at 25°C and a homogenization pressure of 100 MPa to obtain the wave-shaped melon seed core-satellite nanostructure composition.

[0010] The above-described technical solution describes the preparation method for a wave-shaped melon seed core-satellite nanostructure composition.

[0011] The above-described wavy melon seed kernel-satellite nanostructure composition, wherein the content of dehydrobispineol in the wavy melon seed kernel-satellite nanostructure composition is (51.75±3.63) μg·mL. -1 The content of pine methyl methacrylate was (489.11±10.54) μg·mL. -1 .

[0012] The above-described wavy melon seed core-satellite nanostructure composition has the following characteristics: the average particle size of the wavy melon seed core-satellite nanostructure composition is 200-300 nm, the PDI is 0.05-0.30, the absolute potential value is 40-60 mV, and TEM shows that its morphology is a core-satellite structure.

[0013] The above-described bougainvillea seed kernel-satellite nanostructure composition, wherein: the encapsulation rate of dehydrobispineol in the bougainvillea seed kernel-satellite nanostructure composition is 74.31%±4.83%, and the encapsulation rates of bougainvillea methyl ester are 95.62%±3.27%.

[0014] The application of the above-described wavy melon seed core-satellite nanostructure composition in the preparation of drugs for the treatment and / or prevention of autoimmune hepatitis.

[0015] In the application described in the above technical solution, the autoimmune hepatitis refers to Con A-induced autoimmune hepatitis.

[0016] In the application described in the above technical solution, the dosage form of the drug is an oral preparation; preferably, the oral preparation is one of the following: mixture, granules, tablets, pills, or capsules.

[0017] The application described in the above technical solution refers to the use of the bougainvillea seed core-satellite nanostructure composition to reduce inflammatory response and improve liver damage by regulating the NLRP3 / Caspase-1 / IL-1β signaling pathway.

[0018] The present invention has the following beneficial effects: 1. The wave-shaped melon seed core-satellite nanostructure composition of the present invention has a uniform particle size distribution and a stable system; its average particle size is 200-300 nm, PDI is 0.05-0.30, absolute potential value is 40-60 mV, and TEM shows that its morphology is a core-satellite structure. 2. The spinach seed core-satellite nanostructure composition obtained by the present invention has lignans and upper layer fatty oil from spinach as the main active ingredients, and has the characteristics of high stability, significant efficacy, safety and reliability, and low toxicity and side effects. 3. The wave-shaped melon seed core-satellite nanostructure composition of the present invention has good biocompatibility and can reduce inflammatory response and improve liver damage by regulating the NLRP3 / Caspase-1 / IL-1β signaling pathway. Attached Figure Description

[0019] Figure 1 The images show the characterization of the wave-shaped melon seed core-satellite nanostructure composition in Experimental Example 1, where A is the appearance morphology image; B is the particle size distribution image; C is the transmission electron microscope image of MP-SAN; and D is the transmission electron microscope image of SS-SAN. Figure 2 The results show the stability of the wavy seed core-satellite nanostructure composition in Experiment Example 1, where A represents particle size stability at 4℃; B represents DA retention rate; and C represents Her retention rate. Figure 3 The figure shows the results of the CCK8 proliferation experiment in Experiment Example 2, where A represents the cytotoxicity of the composition on Raw264.7 macrophages; and B represents the activity of the composition on LPS-induced Raw264.7 macrophages. Figure 4 This is a diagram illustrating the drug administration method used in the animal experiment of Experiment Example 3; Figure 5 The figure shows the results of the animal experiment in Experiment Example 3, where A is the liver index; B is the serum ALT level; and C is the AST level. Figure 6 Image showing the HE staining results of the liver in Experiment Example 3; Figure 7 The image shows the qRT-PCR results in Experiment 3, where A represents NLRP3; B represents Caspase-1; and C represents IL-1β. Detailed Implementation

[0020] To facilitate understanding of the technical solution of the present invention, the preparation method and application of the wavy melon seed core-satellite nanostructure composition of the present invention will be further explained below with reference to specific embodiments.

[0021] Example 1: Preparation of a composite material of wavy melon seed kernel and satellite nanostructure: The *Pleurotus ostreatus* seed core-satellite nanostructure composition was prepared using "micro-precipitation" and "top-down" techniques. Specifically, an appropriate concentration of traditional Chinese medicine alcohol extract was added to the aqueous decoction, and conditions were controlled to initially form self-assembled nanoparticles. The composition was then prepared using a high-pressure homogenization method. The specific operational steps are as follows: (1) Weigh out the wavy melon seeds; (2) Soak (1) in 12 times the amount of ultrapure water for 30 min, reflux and decoct for 1 h, filter, and keep the dregs for later use; (3) Take the remaining residue of the decoction of the pine melon seeds in step (2), add 8 times the amount of 75% ethanol, extract for 1-2 hours, filter, and set aside. (4) Slowly add the alcohol extract of the pine nut seed residue prepared in step (3) to the decoction of the pine nut seed prepared in step (2), at 800 r·min -1 Stir at a certain speed for 5 minutes to obtain the product; (5) Take the product from step (4), remove the ethanol by rotary evaporation under reduced pressure at 45°C, and concentrate it to a concentration of 0.3 g crude drug·mL. -1 Thus, a novel self-assembled nanoparticle (MP-SAN) of wavy sunflower seeds was obtained. (6) Take the product from step (5) and place it in a high-pressure homogenizer. Homogenize it for 10 cycles at 25°C and a homogenization pressure of 100 MPa to obtain the wavy melon seed core-satellite nanostructure composition (SS-SAN).

[0022] Example 2: Determination of the content of main active ingredients in the wavy melon seed kernel-satellite nanostructure composition: HPLC method for determining the content of dehydrobispineol (DA) and boramine (Her): Instruments and reagents: The instrument used was an LC-20 AD high-performance liquid chromatograph, manufactured by Shimadzu Corporation, Japan; methanol was of chromatographic grade; other reagents were of analytical grade.

[0023] Chromatographic conditions: Inertsil ® ODS-3 column (4.6 × 250 mm, 5 μm); pre-column: GL-CARTRIDGE C18 column; mobile phase: 0.1% phosphoric acid aqueous solution: acetonitrile (77:23); flow rate: 1.0 mL·min -1 Detection wavelength: 280 nm; Column temperature: 40℃; Injection volume: 20 μL.

[0024] Preparation of reference solutions: Accurately weigh appropriate amounts of dehydrobispine alcohol and pinephenanthrene reference standards, and prepare 0.5 mg / mL solutions respectively. -1 DA and 1 mg·mL -1 The Her standard stock solution. Take an appropriate amount of the above standard stock solution, mix, and dilute stepwise to prepare a series of mixed standard solutions of different concentrations.

[0025] Preparation of test solutions: Accurately pipette 1 mL of MP-SAN and SS-SAN solutions into 50 mL volumetric flasks, add an appropriate amount of acetonitrile, and sonicate to dissolve, obtaining MP-SAN test solutions and SS-SAN test solutions. Perform lyophilization on MP-SAN and SS-SAN to obtain lyophilized powders, and further sonicate with acetonitrile to obtain test solutions.

[0026] Accurately pipette 20 μL each of the series of mixed reference standard solutions and the test solution, inject them into the high performance liquid chromatograph, and determine the result.

[0027] The above method demonstrates good repeatability and high accuracy in determining the content of the composition sample, and can be used for the content determination of the composition. The final DA value in MP-SAN was (58.59±2.10) μg·mL. -1 Her was (500.29±10.57) μg·mL -1 The DA content in SS-SAN was (51.75±3.63) μg·mL.-1 Her was (489.11±10.54) μg·mL -1 In MP-SAN, the encapsulation rates of DA and Her are 71.67%±4.92% and 94.13%±3.96%, respectively; in SS-SAN, the encapsulation rates of DA and Her are 74.31%±4.83% and 95.62%±3.27%, respectively.

[0028] The following experimental examples illustrate the beneficial effects of the present invention.

[0029] Experimental Example 1: Characterization of the wavy melon seed core-satellite nanostructure composition: 1. Take MP-SAN and SS-SAN from Example 1, dilute them appropriately with distilled water, and measure their particle size and PDI using a nanoparticle size analyzer. Repeat the measurement 3 times and take the average value.

[0030] 2. TEM observation of MP-SAN and SS-SAN: After diluting the sample appropriately with distilled water, drop it onto a copper grid covered with a carbon film, let it dry naturally, and then observe its morphological characteristics under a transmission electron microscope.

[0031] 3. Take MP-SAN and SS-SAN from Example 1, dilute them appropriately with distilled water, and measure their Zeta potential using a JS94 microelectrophoresis apparatus.

[0032] 4. Take the MP-SAN and SS-SAN from Example 1 and examine their stability.

[0033] 5. Experimental Results: The particle size distribution and morphology of MP-SAN and SS-SAN are as follows: Figure 1 As shown. After concentration, a large amount of fatty acid substances aggregated on the upper layer of MP-SAN. After high-pressure homogenization, the SS-SAN solution exhibited a homogeneous and uniform state, without stratification (as shown). Figure 1 As shown in Figure A). The average particle size of MP-SAN is (495.09 ± 34.37) nm (as shown in Figure A). Figure 1 As shown in B), the PDI is 0.3235 ± 0.0845, the Zeta potential is (-44.04 ± 3.84) mV, and the average particle size of SS-SAN is (262.16 ± 20.91) nm (as shown in B). Figure 1 As shown in Figure B), the PDI was 0.1151 ± 0.0187, and the Zeta potential was (-51.95 ± 0.68) mV. TEM observation revealed that the nanoparticles in MP-SAN exhibited aggregation (as shown in Figure B). Figure 1 As shown in C), the particle size is relatively large; SS-SAN exhibits a core-satellite structure and a uniform morphology (as shown in C). Figure 1 (as shown in D).

[0034] Stability results are as follows Figure 2 As shown, the storage stability at 4℃ indicates that the particle size of MP-SAN gradually increases, while the particle size of SS-SAN shows no significant change (e.g., ...). Figure 2 As shown in A). The retention rates of DA and Her remained uniform and stable over 30 days (as shown in A). Figure 2 B and Figure 2 (as shown in C).

[0035] Experimental Example 2: Cellular compatibility of the wavy melon seed core-satellite nanostructure composition: 1. Cell line: RAW 264.7 macrophages 2. Reagents and equipment: DMEM culture medium, highly inactivated fetal bovine serum, penicillin-streptomycin mixture, CCK-8 kit.

[0036] 3. Experimental methods: (1) Sample preparation: The wave-shaped melon seed core-satellite nanostructure composition prepared in Example 1 was serially diluted with DMEM medium and set aside for later use.

[0037] (2) Determination of RAW264.7 cell viability: RAW 264.7 cells in good growth condition and in the logarithmic growth phase were seeded into 96-well plates, with a cell density of 5 × 10⁶ cells per well. 4 1 cm -2 The 96-well plates were placed in a 37°C, 5% CO2 incubator for adherent culture. Different drugs (SS-SAN: 500, 1000, 2000, 4000 μg crude drug·mL) were used for culture. -1 RAW 264.7 cells were treated with LPS for 24 h. Simultaneously, after cell adhesion, LPS stimulation was applied to different drugs (SS-SAN: 250, 500, and 1000 μg crude drug / mL). -1 After 24 h of treatment, cell viability was detected using the CCK-8 assay.

[0038] 4. Experimental Results: CCK-8 results are as follows Figure 3 As shown, 1000 μg·mL -1 The cellular biocompatibility of the wavy melon seed core-satellite nanostructure composition at certain concentrations is good (e.g., Figure 3 (As shown in A). Simultaneously, SS-SAN can alleviate LPS-induced damage to Raw264.7 cells, reduce apoptosis, and increase cell survival (e.g., as shown in Figure A). Figure 3 As shown in B).

[0039] Experimental Example 3: Pharmacodynamic study of the combination of pine nut kernel and satellite nanostructure: 1. Animals: 48 male C57BL / 6J mice, SPF grade, 4 weeks old, weighing 20±1 g.

[0040] 2. Reagents and equipment: Concanavalin A, prednisone; ALT and AST kits.

[0041] 3. Experimental methods: (1) Model establishment and drug administration: SPF-grade C57BL / 6 mice that passed quarantine were randomly divided into 6 groups of 8 mice each: a control group, a model group, and a prednisone group (5.0 mg / kg). -1 Prednisone), a low-potency (1.25 g·kg) combination of pine nut kernel-satellite nanostructures. -1 , SS-SAN-L), medium (2.50 g·kg -1 (SS-SAN-L) and high (5.0 g·kg) -1 , SS-SAN-H) dosage group.

[0042] After two days of acclimatization feeding, mice were administered the prescribed dose of the drug via gavage daily for seven consecutive days. On day 7, except for the control group, the remaining mice were injected intravenously with 10 mg / kg of the drug. -1 An autoimmune hepatitis (AIH) model was successfully established using Con A solution after 12 hours. See [link to specific method] for details. Figure 4 .

[0043] (2) Determination of the liver coefficient in mice: Mice were weighed before sampling, euthanized by cervical dislocation, dissected, and their livers were removed, wiped with filter paper, weighed again, and the liver coefficient was calculated. Liver coefficient (%) = (liver mass mg / body mass g) × 100%.

[0044] (3) Determination of serum biochemical parameters in mice: Blood was collected from the eyeballs of mice, and the blood was kept at room temperature for 30 min, followed by incubation at 4°C and 1500 r·min. -1 Centrifuge for 15 min under the specified conditions, collect the supernatant serum, store at -20℃, and measure the ALT and AST levels in mouse serum according to the kit instructions.

[0045] (4) HE staining to observe pathological changes in liver tissue: After dissection and treatment, mouse livers were fixed with 4% paraformaldehyde, and tissue sections were prepared by paraffin embedding. Hematoxylin-eosin (HE) staining was performed, and the pathological morphological characteristics of mouse liver tissues in each group were observed under an optical microscope.

[0046] (5) Detection of liver tissue mRNA expression level by qRT-PCR: 50 mg of liver tissue was collected, and 1 mL of Trizol reagent was added to isolate and purify tissue RNA. The mRNA concentration in the sample was determined using a Nano Drop 1000 spectrometer. Following the instructions of the Takara reverse transcription kit, the mixture was prepared using the recommended reaction system and transferred to an eight-tube PCR instrument for reverse transcription. The expression of NLRP3, Caspase-1, and IL-1β was detected using real-time quantitative polymerase chain reaction. The GAPDH gene was used as an internal control, and 2... -△△CT The relative expression level of mRNA was calculated using this method.

[0047] 4. Experimental Results: (1) The liver coefficient results in Experiment Example 3 are as follows Figure 5 As shown in Figure A, compared with the control group, the liver coefficient of mice in the model group was increased. Compared with the model group, both the prednisone group and the high-dose combination could reduce the elevated liver coefficient level in Con A-induced autoimmune hepatitis mice. P <0.05).

[0048] (2) The serum ALT and AST results in Experiment Example 3 are as follows: Figure 5 (B~C) Compared with the blank group, the serum ALT and AST levels in the model group mice were significantly increased, reaching 9.548 times and 2.198 times that of the blank control group, respectively. This indicates that the liver tissue of the model group mice was severely damaged. Similarly, the drug-treated groups could effectively reduce ALT and AST levels, especially the high-dose group of the bougainvillea seed core-satellite nanostructure composition, whose ALT and AST levels were comparable to those of the control group. P The concentration of the protein content (<0.05~0.01) indicates that the combination of the pine seed kernel-satellite nanostructure has good hepatoprotective activity.

[0049] (3) The results of HE staining of the liver in Experiment Example 3 are as follows: Figure 6 As shown, the liver tissue of mice in the normal control group had a clear and intact structure, with hepatocytes of uniform size and no punctate necrosis or inflammatory cell infiltration. In the model group, punctate necrosis and focal necrosis were observed in the liver tissue, accompanied by significant inflammatory cell infiltration. Compared with the model group, the liver tissue lesions in both the prednisone group and the group in the *Polygonum multiflorum* seed core-satellite nanostructure composition group showed varying degrees of improvement.

[0050] (4) The qRT-PCR detection results in Experiment Example 3 are as follows: Figure 7 As shown in Figures A-C, the combination of pine needle-satellite nanostructures significantly reduced the mRNA expression of NLRP3, Caspase-1, and IL-1β in mice with autoimmune hepatitis. P <0.05).

[0051] In summary, this invention prepares a core-satellite nanostructure composition of pine nuts using "micro-precipitation" and "top-down" techniques. This composition exhibits a core-satellite structure, uniform particle size distribution, and system stability. In vitro studies show that the pine nut core-satellite nanostructure composition has good cellular biocompatibility. In vivo studies show that the pine nut core-satellite nanostructure composition can effectively reduce inflammatory responses and improve immune liver injury in mice by regulating the NLRP3 / Caspase-1 / IL-1β signaling pathway.

[0052] The above description is merely a preferred embodiment of the present invention and does not constitute any limitation on the present invention in any form or substance. Any modifications, alterations, and variations made by those skilled in the art without departing from the scope of the present invention using the disclosed technical content are equivalent embodiments of the present invention. Furthermore, any modifications, alterations, and variations made to the above embodiments based on the essential technology of the present invention are still within the scope of the present invention.

Claims

1. A composite material of a wavy melon seed kernel and a satellite nanostructure, characterized in that: The wavy melon seed core-satellite nanostructure composition uses wavy melon seeds as raw material. First, micro-precipitation technology is used, followed by Top-Down technology to form a uniform and stable wavy melon seed core-satellite nanostructure composition. Microprecipitation technology refers to adding an appropriate concentration of alcoholic extract of traditional Chinese medicine to the decoction; Top-Down technology refers to uniformly dispersing the upper fatty acids through high-pressure homogenization.

2. A method for preparing a wave-shaped melon seed core-satellite nanostructure composition, characterized in that: The preparation method includes the following steps: (1) Weigh out the wavy melon seeds; (2) Soak (1) in 12 times the amount of water for 30 min, reflux and decoct for 1 h, filter, and keep the dregs for later use; (3) Take the remaining residue of the decoction of the pine melon seeds in step (2), add 8 times the amount of 75% ethanol, extract for 1-2 hours, filter, and set aside. (4) slowly add the alcohol extract of the residue of the seed of Trichosanthes kirilowii Maxim prepared in step (3) to the water decoction of the seed of Trichosanthes kirilowii Maxim prepared in step (2), stir at a speed of 800 r·min -1 for 5 min, and obtain the product. (5) The product of step (4) was taken, and ethanol was removed by rotary evaporation at 45°C under reduced pressure, and concentrated to a concentration of 0.3 g crude drug mL -1 ; (6) Take the product from step (5), place it in a high-pressure homogenizer, and homogenize it for 10 cycles at 25°C and a homogenization pressure of 100 MPa to obtain the wave-shaped melon seed core-satellite nanostructure composition.

3. The wave-shaped melon seed core-satellite nanostructure composition prepared by the preparation method of claim 2.

4. The wave-shaped melon seed core-satellite nanostructure composition according to claim 1 or claim 3, characterized in that: The content of dehydrobispine alcohol in the wavy melon seed kernel-satellite nanostructure composition is (51.75±3.63) μg·mL. -1 The content of pine methyl methacrylate was (489.11±10.54) μg·mL. -1 .

5. The wave-shaped melon seed core-satellite nanostructure composition according to claim 1 or claim 3, characterized in that: The average particle size of the wavy melon seed core-satellite nanostructure composition is 200-300 nm, the PDI is 0.05-0.30, the absolute potential value is 40-60 mV, and TEM shows that its morphology is a core-satellite structure.

6. The wave-shaped melon seed core-satellite nanostructure composition according to claim 1 or claim 3, characterized in that: The encapsulation rates of dehydrobispine alcohol and pine methyl ester in the aforementioned pine seed kernel-satellite nanostructure composition were 74.31%±4.83% and 95.62%±3.27%, respectively.

7. The use of the wavy melon seed core-satellite nanostructure composition according to claim 1, 3, 4, 5 or 6 in the preparation of drugs for the treatment and / or prevention of autoimmune hepatitis.

8. The application according to claim 7, characterized in that: The autoimmune hepatitis referred to here is Con A-induced autoimmune hepatitis.

9. The application according to claim 7 or 8, characterized in that, The dosage form of the drug is an oral preparation; the preferred oral preparation is one of the following: mixture, granules, tablets, pills or capsules.

10. The application according to any one of claims 7-9, characterized in that: The application refers to the use of the bougainvillea seed core-satellite nanostructure composition to reduce inflammatory response and improve liver damage by regulating the NLRP3 / Caspase-1 / IL-1β signaling pathway.

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