Automatic gram dyeing machine of 3D printing frame structure

Abstract

The invention belongs to the technical field of medical examination technology auxiliary equipment, and particularly relates to an automatic Gram dyeing machine with a 3D printing frame structure, the main structure is customized through 3D modeling software and printed through a fused deposition modeling (FDM) 3D printer, and the automatic Gram dyeing machine comprises a main body frame, a glass slide fixing frame, a silicone tube fixing plate and a switch cover plate; a steering engine fixing clamping position is formed in the main body frame; the steering engine fixing clamping position is used for installing a steering engine; a glass slide fixing frame is mounted on the steering engine; a dyeing tank is arranged in the main body frame; a waste liquid hole communicated with the dyeing tank is formed in one side of the main body frame; according to the invention, the rapid customized production of the equipment framework is realized, the research and development and manufacturing cost is reduced, the assembly and maintenance convenience of the functional module is optimized, the manpower is saved by the automatic process of gram dyeing, the potential health risk of gram dyeing liquor to human bodies is reduced, and the consistency and accuracy of dyeing results are improved.

Description

Technical Field

[0001] This invention belongs to the field of medical testing technology auxiliary equipment, specifically an automatic Gram staining machine with a 3D printed frame structure. Background Technology

[0002] Gram staining is a core method for identifying bacteria in medical microbiology laboratories. Traditional manual procedures are cumbersome and have poor repeatability. Gram staining reagents also pose potential health risks to operators. Existing automated Gram staining equipment mostly uses metal or injection-molded frame structures, which suffer from long processing cycles, high costs, high energy consumption, and low flexibility in structural modifications. Some small and medium-sized laboratories find it difficult to adapt to their personalized testing needs due to the high cost of customized equipment. At the same time, metal frames are heavy and have complex assembly processes, while injection-molded frame molds have long development cycles, making it impossible to quickly respond to the needs of equipment structural optimization and iteration. In addition, the connection between the functional modules and the frame of existing equipment is mostly rigid and fixed, making disassembly and maintenance inconvenient and further increasing the cost of use.

[0003] Therefore, the present invention provides an automated Gram staining machine with a 3D printed frame structure. Summary of the Invention

[0004] In order to overcome the shortcomings of the prior art, at least one technical problem raised in the background art is solved.

[0005] The technical solution adopted by this invention to solve its technical problem is as follows: An automatic Gram staining machine with a 3D printed frame structure, comprising a main frame customized using 3D modeling software and printed using a fused deposition modeling (FDM) 3D printer, a glass slide holder, a silicone tube fixing plate, and a switch cover plate. The printing material used is polyethylene terephthalate copolymer (PETG), which has high structural strength and good chemical resistance. It is printed using slicing parameters of 0.2mm layer height, 3 layers, and 30% infill density. The main frame contains a servo motor fixing slot. The servo motor mounting bracket is used to install the servo motor; the slide holder is mounted on the servo motor, allowing the servo motor to rotate the slide holder 180 degrees; the slide holder is used to hold the slides to be stained; the main frame has a set of motor pump slots, which are interconnected at the bottom, for installing five motor pumps. The inlets of the five motor pumps are connected via silicone tubing for drawing Gram stain and rinsing solution; the silicone tubing fixing plate is mounted on the main frame with screws, positioned above the servo motor mounting bracket, for securing the silicone tubing connected to the outlets of the five motor pumps, and for drawing the solution from the five motor pumps. Gram staining solution and rinsing solution are dropped onto a glass slide on the slide holder below. The main frame has a circuit control compartment and a fan slot for housing the circuit control board and fan. The circuit control compartment is connected to the bottom of the motor pump slot and the servo motor mounting slot. The power cables of the five motor pumps and the servo motor power signal cables can extend through the connected space to the circuit control compartment and connect to the circuit control board. The circuit control board is powered via a USB power cable using a 5V 2A output adapter. The circuit control board receives switch signals to control the five motor pumps, servo motors, and fan to perform the automatic Gram staining process. The fan's function is to dissipate heat from the circuit control board while simultaneously drying the stained slides on the slide holder. The switch cover is located above the circuit control compartment and is fixed to the main frame with screws. The switch cover has a switch fixing position for fixing the switch, which is connected to the circuit control board. Its function is to send a command to the circuit control board when the switch is pressed to execute the automatic Gram staining program. A staining pool is provided inside the main frame to collect the waste liquid rinsed during the staining process. A waste liquid hole is provided on one side of the main frame to drain the waste liquid from the staining pool by gravity.

[0006] Preferably, a guide rail is fixedly connected to the main frame, and a sliding groove is formed on the side of the guide rail near the slide holder. A slider is slidably connected to the inner wall of the sliding groove, and a lead screw is internally threaded to the slider. The lead screw is rotatably connected to the sliding groove. A motor for driving the lead screw to rotate is provided at the top of the guide rail. A rectangular plate is provided on the side of the slider near the slide holder, and a sponge block is fixedly connected to the side of the rectangular plate away from the slider.

[0007] Preferably, a round rod is fixedly connected to the side of the slider near the rectangular plate, the end of the round rod away from the slider is open, a sliding rod is slidably connected inside the round rod, the end of the sliding rod away from the slider is fixedly connected to the rectangular plate, and a moving component for controlling the movement of the sliding rod is provided on the guide rail.

[0008] Preferably, the moving component includes a hollow groove formed in the guide rail, a first conduit connecting the hollow groove and the round rod, a first control valve being provided on the first conduit, a connecting pipe communicating with the hollow groove being provided on one side of the guide rail, a second control valve being provided in the connecting pipe, a first spring being fixedly connected between the side of the slide rod away from the rectangular plate and the inner wall of the round rod, a magnetic block magnetically attracted to the slider being slidably connected in the hollow groove, and an exhaust component being provided on the guide rail.

[0009] Preferably, the rectangular plate has a cavity inside, and multiple sets of circular holes are opened on the side of the rectangular plate away from the slide rod, so that gas can enter the cavity when the exhaust assembly exhausts gas.

[0010] Preferably, the exhaust assembly includes a second conduit, one end of which is in communication with the cavity, and the other end of which is away from the cavity and in communication with the hollow groove.

[0011] Preferably, a set of rectangular grooves are formed inside the sponge block, and an arc-shaped elastic sheet is fixedly connected inside the rectangular groove. A second spring is fixedly connected between the side of the elastic sheet near the slide rod and the inner wall of the rectangular groove.

[0012] The beneficial effects of this invention are as follows: 1. Compared with manual Gram staining, the present invention has a high degree of automation, improves work efficiency, saves manpower, improves staining quality, effectively reduces human operation errors, and relatively reduces the potential health impact of Gram staining solution on personnel.

[0013] 2. Compared with existing Gram staining machines, the main frame and accessories of the Gram staining machine made by 3D modeling and 3D printing have a simple structure, short manufacturing cycle, low cost, convenient disassembly and maintenance, low manufacturing cost, are lightweight and compact, have low energy consumption, and can be customized according to requirements. Attached Figure Description

[0014] The invention will now be further described with reference to the accompanying drawings.

[0015] Figure 1 This is a schematic diagram of the automatic Gram staining machine of the present invention; Figure 2 This is a schematic diagram of the assembled electrical components in this invention; Figure 3 This is a top view of the automatic Gram staining machine of the present invention; Figure 4This is a schematic diagram of the guide rail structure on the main frame in this invention; Figure 5 This is a schematic diagram of the guide rail structure in this invention; Figure 6 This is a schematic diagram of the internal structure of the guide rail and the round rod in this invention; Figure 7 yes Figure 6 A partial structural diagram.

[0016] In the diagram: 1. Main frame; 2. Slide holder; 3. Silicone tube holder; 4. Switch cover; 5. Waste liquid outlet; 6. Staining tank; 7. Servo motor mounting position; 8a. Motor pump slot; 8b. Motor pump slot; 8c. Motor pump slot; 8d. Motor pump slot; 8e. Motor pump slot; 9. Fan slot; 10. Circuit control compartment; 11. Power cable tray; 12. Switch mounting position; 13a. Unclosed circular hole; 13b. Unclosed circular hole; 13c. Unclosed circular hole; 13d. Unclosed circular hole; 13e. Unclosed circular hole; 14a. Electric... 14b, Motor pump; 14c, Motor pump; 14d, Motor pump; 14e, Motor pump; 15, Fan; 16, Switch; 17, Servo motor; 18, Circuit control board; 19, USB power cable; 20, Guide rail; 21, Slider; 22, Rectangular plate; 23, Sponge block; 24, Lead screw; 25, Motor; 26, Slide groove; 27, Round rod; 28, Slide rod; 29, Hollow groove; 30, First conduit; 31, Connecting pipe; 32, Second conduit; 33, Magnetic block; 34, Cavity; 35, Round hole; 36, Rectangular groove; 37, Elastic sheet. Detailed Implementation

[0017] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0018] Example 1: As Figure 1-3 As shown in the embodiment of the present invention, an automatic Gram staining machine with a 3D printed frame structure includes a main frame 1 formed by 3D modeling and 3D printing, a glass slide holder 2, a silicone tube fixing plate 3, a switch cover plate 4, and electrical components including motor pumps 14a-14e, a fan 15, a switch 16, a servo motor 17, a circuit control board 18, and a USB power cable 19.

[0019] The 3D modeling and 3D printing process: The main frame 1, slide holder 2, silicone tube holder 3, and switch cover 4 are drawn using Fusion360 modeling software. The main frame 1 contains a staining tank 6, a servo motor mounting slot 7, a fan slot 9, and a circuit control compartment 10. The top of the main frame 1 has a set of motor pump slots 8a-8e and a power cable slot 11, and the circuit control compartment 10 communicates with the servo motor mounting slots 7, motor pump slots 8a-8e, and fan slot 9. One side of the main frame 1 has a waste liquid hole 5 connected to the staining tank 6. The slide holder 2 has slots for placing slides and holes for connecting to the servo motor 17. The silicone tube holder 3 has a set of unclosed circular holes 13a-13e. The switch cover 4 has a switch mounting position 12. The model was exported in STL format using Fusion360 modeling software, imported into the slicing software of the 3D printer, and the slicing parameters were set as follows: layer height 0.2mm, 3-layer walls, 30% infill density, and automatic tree support. The model was then printed using polyethylene terephthalate copolymer (PETG) filament.

[0020] The electrical components are installed as follows: the circuit control board 18 is placed inside the circuit control compartment 10 of the main frame 1; the USB power cable 19 is connected to the circuit control board 18 to supply power, and extends to the outside of the main frame 1 through the power cable groove 11 on the main frame 1; the servo motor 17 is installed on the servo motor fixing slot 7 inside the main frame 1, and the power signal line of the servo motor 17 is connected to the circuit control board 18 through the space communicating with the circuit control compartment 10. The fan 15 is installed in the fan slot 9 within the main frame 1, and the power cord of the fan 15 is connected to the circuit control board 18 within the circuit control compartment 10. The motor pumps 14a, 14b, 14c, 14d, and 14e are respectively installed in the motor pump slots 8a, 8b, 8c, 8d, and 8e on the main frame 1, and their power cords are all connected to the circuit control board 18 through a space at the bottom. The power signal line of the switch 16 is connected to the circuit control board 18, and the switch 16 is installed on the switch cover 4 with screws.

[0021] Installation of the slide holder 2: Power on the circuit control board 18 beforehand, set the initial position of the servo motor 17 to 0 degrees, then fix the slide holder 2 horizontally on the rotating shaft of the servo motor 17 and tighten it with screws.

[0022] Installation of the silicone tube fixing plate 3: The silicone tube fixing plate 3 is installed on the reserved hole in the main frame 1 by screws, and is located above the slide fixing bracket 2. Five 17mm long silicone tubes are fixed at one end to the outlets of motor pumps 14a, 14b, 14c, 14d, and 14e, respectively. The other end of the silicone tube connected to the outlet of motor pump 14a is inserted into the unclosed circular hole 13a on the silicone tube fixing plate 3. The other end of the silicone tube connected to the outlet of motor pump 14b is inserted into the unclosed circular hole 13b on the silicone tube fixing plate 3. The other end of the silicone tube connected to the outlet of motor pump 14c is inserted into the unclosed circular hole 13c on the silicone tube fixing plate 3. The other end of the silicone tube connected to the outlet of motor pump 14d is inserted into the unclosed circular hole 13d on the silicone tube fixing plate 3. The other end of the silicone tube connected to the outlet of motor pump 14e is inserted into the unclosed circular hole 13e on the silicone tube fixing plate 3.

[0023] Installation of the switch cover plate 4: The switch cover plate 4 is installed on the reserved hole in the main frame 1 by screws, located above the circuit control compartment 10 and the fan slot 9.

[0024] Finally, one end of each of the five 34mm long silicone tubes is fixed to the inlet of motor pumps 14a, 14b, 14c, 14d, and 14e, respectively, and the other ends of the five silicone tubes are inserted into the corresponding Gram staining solution and rinsing solution. Thus, the automatic Gram staining machine with a 3D printed frame structure described in Embodiment 1 of this invention is assembled.

[0025] The present invention discloses an automatic Gram staining machine operation method for a 3D printed frame structure: 1. Installation of Gram staining solution and rinsing solution: Insert the silicone tube connected to the inlet of motor pump 14a into the rinsing solution, insert the silicone tube connected to the inlet of motor pump 14b into Gram staining solution A, insert the silicone tube connected to the inlet of motor pump 14c into Gram staining solution B, insert the silicone tube connected to the inlet of motor pump 14d into Gram staining solution C, and insert the silicone tube connected to the inlet of motor pump 14e into Gram staining solution D.

[0026] 2. When the equipment is powered on and initialized, the circuit control board 18 controls the servo motor 17 to drive the slide holder 2 to rotate 70 degrees counterclockwise from the initial position of 0 degrees, so that the slide to be stained can be loaded into the slide holder 2.

[0027] 3. Press switch 16 to execute the automated Gram staining process controlled by the program.

[0028] The automated Gram staining process is as follows: Procedure 1: When the circuit control board 18 receives the signal from the switch 16, it controls the servo motor 17 to rotate the glass slide on the slide holder 2 clockwise by 70 degrees to the initial position of 0 degrees.

[0029] Procedure 2: The motor pump 14b rotates forward to add Gram stain A through the silicone tube onto the slide on the slide holder 2. After the addition is complete, wait for 10 seconds.

[0030] Procedure 3: The motor pump 14b reverses to return Gram stain solution A in the silicone tube to the Gram stain solution A bottle. At the same time, the motor pump 14a rotates forward to drip the rinsing solution onto the slide on the slide holder 2 through the silicone tube. After waiting for 10 seconds, the servo motor 17 drives the slide on the slide holder 2 to rotate 180 degrees counterclockwise and then 180 degrees clockwise to the initial position 0 degrees. At this time, the motor pump 14a stops working. The servo motor 17 drives the slide on the slide holder 2 to rotate 180 degrees counterclockwise and then 180 degrees clockwise to the initial position 0 degrees to shake off the excess rinsing solution on the surface of the slide.

[0031] Step 4: The motor pump 14c rotates forward to add Gram stain B through the silicone tube onto the slide on the slide holder 2. After the addition is complete, wait 10 seconds.

[0032] Step 5: Motor pump 14c reverses to return Gram stain solution B in the silicone tube to the Gram stain solution B bottle. At the same time, motor pump 14a rotates forward to drip the rinsing solution onto the slide on the slide holder 2 through the silicone tube. After waiting for 10 seconds, servo motor 17 drives the slide on the slide holder 2 to rotate 180 degrees counterclockwise and then 180 degrees clockwise back to the initial position of 0 degrees. At this time, motor pump 14a stops working. Servo motor 17 drives the slide on the slide holder 2 to rotate 180 degrees counterclockwise and then 180 degrees clockwise back to the initial position of 0 degrees to shake off the excess rinsing solution on the surface of the slide.

[0033] Procedure 6: The motor pump rotates forward 14d to add Gram stain C through the silicone tube onto the slide on the slide holder 2. After the addition is complete, wait 20 seconds.

[0034] Step 7: Motor pump 14d reverses to return Gram stain solution C in the silicone tube to the Gram stain solution C bottle. At the same time, motor pump 14a rotates forward to drip the rinsing solution onto the slide on the slide holder 2 through the silicone tube. After waiting for 10 seconds, servo motor 17 drives the slide on the slide holder 2 to rotate 180 degrees counterclockwise and then 180 degrees clockwise back to the initial position 0 degrees. At this time, motor pump 14a stops working. Servo motor 17 drives the slide on the slide holder 2 to rotate 180 degrees counterclockwise and then 180 degrees clockwise back to the initial position 0 degrees to remove excess rinsing solution from the surface of the slide.

[0035] Procedure 8: The motor pump 14e rotates forward to add Gram stain solution D through the silicone tube onto the slide on the slide holder 2. After the addition is complete, wait 10 seconds.

[0036] Step 9: Motor pump 14e reverses to return Gram stain solution D in the silicone tube to the Gram stain solution D bottle. At the same time, motor pump 14a rotates forward to drip the rinsing solution onto the slide on the slide holder 2 through the silicone tube. After waiting for 10 seconds, servo motor 17 drives the slide on the slide holder 2 to rotate 180 degrees counterclockwise and then 180 degrees clockwise to the initial position 0 degrees. At this time, motor pump 14a reverses to return the rinsing solution to the rinsing solution bottle. Servo motor 17 again drives the slide on the slide holder 2 to rotate 180 degrees counterclockwise and then 180 degrees clockwise to the initial position 0 degrees to remove excess rinsing solution from the surface of the slide.

[0037] Step 10: Servo motor 17 drives the slide on slide holder 2 to rotate 90 degrees counterclockwise, and fan 15 starts to rotate to help dry the slide for 3 minutes.

[0038] Procedure 11: The servo motor 17 drives the slide on the slide holder 2 to rotate 20 degrees clockwise for easy removal and loading of the slide. After that, the fan 15 is turned off, and the entire Gram staining process is completed. The slide can then be removed and examined under a microscope.

[0039] Example 2: Figures 4 to 7As shown in the comparative embodiment one, another embodiment of the present invention is as follows: a guide rail 20 is fixedly connected to the main frame 1. A groove 26 is formed on the side of the guide rail 20 near the slide holder 2. A slider 21 is slidably connected to the inner wall of the groove 26. A lead screw 24 is internally threaded to the slider 21. The lead screw 24 is rotatably connected to the groove 26. A motor 25 for driving the lead screw 24 is provided at the top of the guide rail 20. A rectangular plate 22 is provided on the side of the slider 21 near the slide holder 2. A sea-shaped plate is fixedly connected to the side of the rectangular plate 22 away from the slider 21. The slider 21 is fixedly connected to a round rod 27 on the side near the rectangular plate 22. The end of the round rod 27 away from the slider 21 is open. A sliding rod 28 is slidably connected inside the round rod 27. The end of the sliding rod 28 away from the slider 21 is fixedly connected to the rectangular plate 22. A moving component for controlling the movement of the sliding rod 28 is provided on the guide rail 20. The moving component includes a hollow groove 29 opened in the guide rail 20. A first conduit 30 communicates between the hollow groove 29 and the round rod 27. A first control valve is provided on the first conduit 30. A connecting pipe 31 communicating with the hollow groove 29 is provided, and a second control valve is provided inside the connecting pipe 31. A first spring is fixedly connected between the side of the slide rod 28 away from the rectangular plate 22 and the inner wall of the round rod 27. A magnetic block 33 that magnetically attracts the slider 21 is slidably connected inside the hollow groove 29. An exhaust assembly is provided on the guide rail 20. A cavity 34 is opened inside the rectangular plate 22, and multiple sets of round holes 35 are opened on the side of the rectangular plate 22 away from the slide rod 28. When the exhaust assembly exhausts, the gas can enter the cavity 34. The exhaust assembly includes a second conduit 32, the second... One end of the conduit 32 is connected to the cavity 34, and the end of the second conduit 32 away from the cavity 34 is connected to the hollow groove 29. When the magnetic block 33 moves downward, the magnetic block 33 will push the gas in the hollow groove 29, allowing the gas to enter the cavity 34 through the second conduit 32. When the magnetic block 33 moves upward, the gas in the cavity 34 can flow back into the hollow groove 29. A set of rectangular grooves 36 are opened in the sponge block 23. An arc-shaped elastic sheet 37 is fixedly connected in the rectangular groove 36. A second spring is fixedly connected between the side of the elastic sheet 37 near the slide rod 28 and the inner wall of the rectangular groove 36.

[0040] Working principle: The motor 25 is connected to the circuit control board 18. In the first implementation of this application, after the automatic Gram staining process is completed, the circuit control board 18 controls the servo motor 17 to rotate the slide on the slide holder 2 from the initial position of 0 degrees counterclockwise by 90 degrees to the vertical position. At this time, the circuit control board 18 can control the motor 25 to drive the lead screw 24 to rotate. The output end of the motor 25 can rotate in both directions, so that the lead screw 24 can control the slider 21 to move up and down. At this time, the slider 21 can control the sponge block 23 to wipe the residual rinsing solution on the slide on the slide holder 2, which greatly improves the subsequent... The efficiency of the fan in drying the slides on the slide holder 2; the movable component can control the slide bar 28 to move closer to the slide holder 2. At this time, the slide bar 28 will push the rectangular plate 22, so that the rectangular plate 22 drives the sponge block 23 to contact the slides on the slide holder 2. Then, the rectangular plate 22 is controlled to move up and down so that the sponge block 23 can wipe the rinsing liquid on the slides on the slide holder 2. After wiping, the movable component controls the slide bar 28 to return to its original position. At this time, the rectangular plate 22 will move away from the slide holder 2 to prevent the rectangular plate 22 from obstructing the rotation of the slide holder 2. In this embodiment, when the slide bar 28 needs to move closer to the slide holder 2, the second control valve can be closed and the first control valve opened. Then, the slider 21 is controlled to move upward. At this time, the slider 21 will drive the magnetic block 33 to move upward. The magnetic block 33 will push the gas above it into the first conduit 30, and then into the round rod 27 to push the slide bar 28, causing the slide bar 28 to move closer to the slide holder 2. After moving to the appropriate position, the first control valve is closed. At this time, the round rod 27 is in a sealed space, and the slide bar 28 cannot move. Then, the second control valve is opened. At this time, the slider 21 moves up and down, and the magnetic block 33 also moves up and down. At this time, the gas in the hollow groove 29 can circulate through the exhaust assembly and the connecting pipe 31. When the slide bar 28 needs to be reset, only the second control valve needs to be opened, and then the first spring will pull the slide bar 28 to reset. In this application, when the slider 21 moves up and down, the magnetic block 33 will move up and down in the hollow groove 29. The gas below block 33 passes through the exhaust assembly, allowing it to be discharged into cavity 34. This allows the gas to be blown from the round hole 35 onto the sponge block 23, and then onto the slide holder 2, thus drying both the slide holder 2 and the sponge block 23. When the magnetic block 33 moves downwards, it pushes the gas in the hollow groove 29, allowing it to enter cavity 34 through the second conduit 32. When the magnetic block 33 moves upwards, it allows the gas to... The gas in cavity 34 flows back into hollow groove 29; when the gas is blown out through round hole 35, the gas blows the elastic sheet 37, causing the elastic sheet 37 to deform. At this time, the elastic sheet 37 will squeeze the sponge block 23. When the round hole 35 stops blowing air, the second spring will pull down the elastic sheet 37 to reset it. At the same time, the elastic sheet 37 will shake, which will drive the sponge block 23 to shake, thereby shaking off and squeezing off the rinsing liquid wiped on the sponge block 23, improving the drying efficiency of the sponge block 23 itself.

[0041] The terms "front," "back," "left," "right," "top," and "bottom" all refer to the figures in the accompanying drawings. Figure 1 Based on the perspective of the observer, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0042] In the description of this invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the scope of protection of this invention.

[0043] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. An automatic Gram staining machine with a 3D-printed frame structure, comprising a main frame (1), a slide holder (2), a silicone tube fixing plate (3), and a switch cover plate (4); characterized in that: Customized using 3D modeling software and printed using a fused deposition modeling (FDM) 3D printer, the printing material used is polyethylene terephthalate copolymer (PETG); the main frame (1) is provided with a servo motor fixing slot (7), which is used to install a servo motor (17), and a glass slide fixing bracket (2) is installed on the servo motor (17); a staining pool (6) is provided inside the main frame (1); a set of motor pump slots (8a-8e) is provided on the top surface of the main frame (1), and a circuit control compartment (10) and a fan slot (9) are provided inside the main frame (1); a waste liquid hole (5) communicating with the staining pool (6) is provided on one side of the main frame (1); the silicone tube fixing plate (3) is fixed to the main frame (1) with screws; the switch cover plate (4) is fixed to the main frame (1) with screws.

2. The automatic Gram staining machine for a 3D printed frame structure according to claim 1, characterized in that: The switch cover (4) is located above the circuit control compartment (10), and a switch fixing position (12) is provided on the switch cover (4).

3. The automatic Gram staining machine for a 3D printed frame structure according to claim 1, characterized in that: The silicone tube fixing plate (3) has a set of unclosed circular holes (13a-13e) for fixing the silicone tube, located above the slide holder (2).

4. The automatic Gram staining machine for a 3D printed frame structure according to claim 1, characterized in that: The slide holder (2) can rotate 180 degrees under the drive of the servo motor (17).

5. An automatic Gram staining machine for a 3D printed frame structure according to claim 1, characterized in that: A guide rail (20) is fixedly connected to the main frame (1). A groove (26) is provided on the side of the guide rail (20) near the slide holder (2). A slider (21) is slidably connected to the inner wall of the groove (26). A lead screw (24) is threadedly connected to the slider (21). The lead screw (24) is rotatably connected to the groove (26). A motor (25) for driving the lead screw (24) to rotate is provided at the top of the guide rail (20). A rectangular plate (22) is provided on the side of the slider (21) near the slide holder (2). A sponge block (23) is fixedly connected to the side of the rectangular plate (22) away from the slider (21).

6. An automatic Gram staining machine for a 3D printed frame structure according to claim 5, characterized in that: A round rod (27) is fixedly connected to the side of the slider (21) near the rectangular plate (22). The end of the round rod (27) away from the slider (21) is open. A slide rod (28) is slidably connected inside the round rod (27). The end of the slide rod (28) away from the slider (21) is fixedly connected to the rectangular plate (22). A moving component for controlling the movement of the slide rod (28) is provided on the guide rail (20).

7. An automatic Gram staining machine for a 3D printed frame structure according to claim 6, characterized in that: The moving component includes a hollow groove (29) formed in the guide rail (20), a first conduit (30) connecting the hollow groove (29) and the round rod (27), a first control valve being provided on the first conduit (30), a connecting pipe (31) communicating with the hollow groove (29) being provided on one side of the guide rail (20), a second control valve being provided in the connecting pipe (31), a first spring being fixedly connected between the side of the slide rod (28) away from the rectangular plate (22) and the inner wall of the round rod (27), a magnetic block (33) magnetically attracted to the slider (21) being sealed and slidably connected in the hollow groove (29), and an exhaust component being provided on the guide rail (20).

8. An automatic Gram staining machine for a 3D printed frame structure according to claim 7, characterized in that: The rectangular plate (22) has a cavity (34) inside. Multiple sets of round holes (35) are opened on the side of the rectangular plate (22) away from the slide bar (28). When the exhaust assembly exhausts, the gas can enter the cavity (34).

9. An automatic Gram staining machine for a 3D printed frame structure according to claim 8, characterized in that: The exhaust assembly includes a second conduit (32), one end of which is connected to a cavity (34), and the other end of which is connected to a hollow groove (29).

10. An automatic Gram staining machine for a 3D printed frame structure according to claim 8, characterized in that: A set of rectangular grooves (36) are provided in the sponge block (23). An arc-shaped elastic sheet (37) is fixedly connected in the rectangular groove (36). A second spring is fixedly connected between the side of the elastic sheet (37) near the slide rod (28) and the inner wall of the rectangular groove (36).

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