Algae testing apparatus with pre-treatment function and testing method

Abstract

The application discloses an alga testing device with pretreatment function and a testing method, and relates to the technical field of alga testing, which comprises a testing host and a pretreatment device. The application sequentially completes feeding, oscillation wall breaking extraction, centrifugal separation, flow guiding filtration and detection output in the same testing host, reduces manual transfer and multi-device switching, and improves batch detection efficiency and result consistency. Displacement control is performed on the two-way clutch mechanism through the blending mechanism, so that the same pretreatment device realizes quick and reliable switching between the oscillation mode and the centrifugal mode without changing the power source and the bearing, thereby connecting the wall breaking extraction and the centrifugal separation into a continuous controllable process. In the oscillation mode, the end assembly drives the two-way clutch mechanism to reciprocate, drives the bearing and the mixed solution in the double-cavity centrifugal tube to reciprocate and oscillate, breaks the alga cell wall through the glass grinding beads, promotes the release of fucoxanthin and fully mixes the solution, and improves the extraction efficiency.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of algal testing, in particular to an algal testing device with a pre-treatment function and a testing method. BACKGROUND

[0002] An algal testing device is a special instrument for qualitative and quantitative analysis of target components in marine or freshwater algal samples. The algal sample system often has the characteristics of complex components, multiple background interference and large target content difference. The testing device usually combines standard substances and calibration procedures to stably collect sample signals and process data output to obtain reliable test results.

[0003] The existing testing devices for fucoidan and other algal components generally lack a front-end pre-treatment device integrated with the detection host. The processing flow has low integration, and the sample still needs to rely on external oscillators and centrifuges and other dispersed equipment to complete the cell wall breaking and solid-liquid separation before detection, resulting in long operation process, frequent equipment switching, multiple sample transfer times, easy cross contamination and batch differences. Even if some devices integrate oscillation or centrifugation units, they are mostly simple combinations of single functions, lack reliable switching mechanisms for cell wall breaking and centrifugation conditions, and lack synchronous control of flow path opening and closing, still relying on manual cooperation, with low automation level, and difficult to meet the continuous and standardized rapid detection needs of algal samples. SUMMARY

[0004] The purpose of the present application is to provide an algal testing device with a pre-treatment function and a testing method to solve the problem of low integration of the pre-treatment process of the existing testing device.

[0005] To achieve the above purpose, the present application provides the following technical scheme: an algal testing device with a pre-treatment function, comprising a testing host and a pre-treatment device, a detection module is arranged in the testing host, a cleaning module is arranged in the testing host, and an electric lifting device is arranged on the testing host.

[0006] The pre-treatment device comprises a double-drive mechanism, a supporting member and two groups of magnetic attraction swing mechanisms. The double-drive mechanism is installed on the testing host. A blending mechanism is installed at the bottom end of the supporting member. A bidirectional clutch mechanism is symmetrically and slidingly installed at the bottom end of the supporting member. The bidirectional clutch mechanism is movably connected with the blending mechanism. A plurality of double-cavity centrifuge tubes are installed on the supporting member. The magnetic attraction swing mechanisms are symmetrically installed on the testing host. The blending mechanism is used to control and adjust the disconnection of the bidirectional clutch mechanism and the magnetic attraction swing mechanism. The blending mechanism is also used to control and adjust the disconnection of the bidirectional clutch mechanism and the double-drive mechanism.

[0007] A detection module is arranged in the testing host to determine the fucoxanthin content in the extracted liquid. A control system is arranged in the testing host to control the entire testing device.

[0008] Further, the double-cavity centrifugal tube comprises a tube body, the tube body is installed on a support, an end cover assembly is installed on the tube body, a connecting piece is installed on the end cover assembly, and a switch assembly is arranged on the connecting piece;

[0009] The double-drive mechanism comprises a motor, the motor is installed on the test host, and an end head assembly is installed on the output shaft of the motor;

[0010] The magnetic attraction swing mechanism comprises a sliding seat, the sliding seat is installed on the test host, a magnetic attraction sliding head is slidingly installed on the sliding seat, and a sliding assembly is installed between the magnetic attraction sliding head and the sliding seat.

[0011] Further, the bidirectional clutch mechanism comprises a moving assembly, the moving assembly is slidingly installed at the bottom end of the support, and a clutch assembly is installed on the moving assembly;

[0012] The sliding assembly comprises a sliding rod, the sliding rod is connected with the magnetic attraction sliding head, the sliding rod is slidingly connected with the sliding seat, and an elastic piece is installed between the magnetic attraction sliding head and the sliding seat.

[0013] Further, a plurality of limiting sliding grooves are arranged on the support, the bidirectional clutch mechanism is slidingly installed in the limiting sliding grooves, a plurality of collection grooves are arranged on the support in a ring shape, a plurality of cleaning grooves are arranged on the support in a ring shape, the cleaning grooves are located on the inner side of the collection grooves, and the number of the collection grooves is consistent with that of the cleaning grooves;

[0014] The tube body is internally provided with a wall-breaking cavity, a centrifugal cavity and an overflow channel, the wall-breaking cavity and the centrifugal cavity are communicated through the overflow channel, the horizontal height of one end of the overflow channel communicated with the wall-breaking cavity is lower than that of the other end communicated with the centrifugal cavity, a filter plate is arranged in the overflow channel, a first discharge port is arranged at the bottom end of the wall-breaking cavity, a first valve is arranged at the first discharge port, a filter screen is further arranged at the first discharge port, a second discharge port is arranged at the bottom end of the centrifugal cavity, a second valve is arranged at the second discharge port, the first discharge port is aligned with the cleaning groove, and the second discharge port is aligned with the collection groove.

[0015] Further, the end cover assembly comprises a first end cover and a second end cover, the first end cover is installed on the wall-breaking cavity, the second end cover is installed on the centrifugal cavity, a first connecting port is arranged on the first end cover, a second connecting port is arranged on the second end cover, and the connecting piece is connected with the first end cover and the second end cover at two ends respectively;

[0016] The switch assembly comprises an electric telescopic rod, the electric telescopic rod is installed on the connecting piece, an intercepting plate is installed on the output shaft of the electric telescopic rod, and the intercepting plate is slidingly connected with the overflow channel.

[0017] The test host is internally provided with a feeding module, before testing, the first valve and the second valve are in closed state, the intercepting plate intercepts the overflow channel as a whole, so that the wall breaking cavity and the centrifugal cavity are separated. The control system respectively inputs the algal sample, anhydrous ethanol, glass grinding beads and other medicines and reagents into the first connecting port by the feeding module in proportion, and the mixed solution enters the wall breaking cavity from the first connecting port.

[0018] The test host is internally provided with a cleaning module, after the extract is discharged, the control system injects cleaning liquid into the first connecting port and the second connecting port through the cleaning module, then adjusts the pretreatment device to the oscillation mode, and the cleaning liquid cleans the inside of the double-cavity centrifugal tube under the action of vibration, after cleaning, the control system opens the first valve and the second valve, and the cleaning liquid falls from the first discharge port and the second discharge port, respectively flows into the waste liquid collection box in the cleaning module through the collection tank and the cleaning tank, so that the cleaning of the double-cavity centrifugal tube is completed; at the same time, the filter screen arranged on the first discharge port prevents the glass grinding beads from falling, so that the glass grinding beads are retained, and the cleaning liquid cleans the glass grinding beads at the same time of cleaning the double-cavity centrifugal tube, so that the glass grinding beads can be recycled without being taken out for cleaning, and the batch test efficiency is improved.

[0019] Further, the moving assembly includes a sliding block slidingly installed in a limiting sliding groove, and a sliding column installed on the sliding block and movably connected with the blending mechanism;

[0020] The clutch assembly includes a clutch block, the clutch block is provided with an embedded strip, the clutch block is connected with the sliding column, and a clutch wheel is rotatably installed on the clutch block and provided with a convex ring.

[0021] After the mixed solution enters the wall breaking cavity, the control system makes the pretreatment device enter the oscillation mode through the blending mechanism, then the control system starts the motor, the output shaft of the motor drives the end head assembly to rotate, when the driving wheel on the end head assembly rotates to the position of the clutch wheel, the clutch wheel is extruded, the clutch wheel drives the magnetically attracted sliding head locked therewith to slide on the sliding seat after being extruded, the magnetically attracted sliding head extrudes the spring and drives the sliding rod to stably slide on the sliding seat at the same time, when the driving wheel rotates away from the position of the clutch wheel, the pressure on the clutch wheel disappears, the spring drives the bidirectional clutch mechanism to slide reversely through the magnetically attracted sliding head, so as to make the bidirectional clutch device drive the supporting piece thereon to reciprocate, the supporting piece drives the mixed solution in the double-cavity centrifugal tubes thereon to reciprocate and oscillate, in the oscillation process, the glass grinding beads break the cell wall of the algal sample, so that the fucoxanthin is released from the cell wall, the released fucoxanthin is mixed with the solution, so that the preliminary extraction of the fucoxanthin is realized.

[0022] After the preliminary extraction of fucoxanthin is completed, the control system switches the pretreatment device to the centrifugal mode, and starts the motor. The motor output shaft drives the end head assembly, and the end head assembly drives the two-way clutch mechanism to rotate quickly. The two-way clutch mechanism drives the supporting piece to rotate, and the supporting piece drives several double-cavity centrifugal tubes to rotate. Under the action of centrifugation, the fucoxanthin extract is thrown from the broken wall cavity into the overflow channel, and then enters the centrifugal cavity through the filter plate. The solid materials such as glass grinding beads with heavier weight are retained in the broken wall cavity. A small part of the solid materials enters the overflow channel with the extract, and then falls back into the broken wall cavity after centrifugation under the cooperation of the upward structure design of the overflow channel and the blocking effect of the filter plate, so as to complete the centrifugal separation of the extract and avoid the solid materials in the mixed solution entering the centrifugal cavity.

[0023] After centrifugation, the control system opens the second discharge port, and the extract passes through the second discharge port and the collection tank into the flow guide mechanism in the test host. The flow guide mechanism filters the extract through the organic filter membrane and then delivers it to the detection module for testing, thereby completing the automatic extraction and testing of fucoxanthin.

[0024] Further, the magnetic sliding head is provided with an electromagnet, and the magnetic sliding head is provided with an embedded groove. The embedded groove is used for mutual embedding with the embedded strip. The embedded strip is made of an adsorbable material. The bottom end of the magnetic sliding head is provided with a bottom support.

[0025] The end head assembly comprises an end head block. The two sides of the end head block are provided with semicircular grooves. The semicircular grooves are provided with recesses. A driving wheel is rotatably installed on the end head block. The driving wheel is provided with a concave ring. The concave ring can be mutually embedded with the convex ring. The semicircular groove can be mutually embedded with the clutch wheel. The recess can be mutually embedded with the convex ring.

[0026] Further, the blending mechanism comprises a cylinder and a blending disc. One end of the cylinder is rotatably installed at the bottom end of the supporting piece. The blending disc is rotatably installed at the bottom end of the supporting piece. The output shaft of the cylinder is rotatably connected with the blending disc. The blending disc is provided with a curved groove. The sliding column is movably connected with the curved groove.

[0027] The control system starts the cylinder. The cylinder drives the blending disc to deflect at the bottom end of the supporting piece through the output shaft. When the blending disc deflects, the sliding column is driven to move through the curved groove. Since the sliding block connected with the sliding column can only slide linearly in the limiting sliding groove, the entire two-way clutch mechanism can only reciprocate along the direction of the limiting sliding groove under the driving action of the blending disc. The two two-way clutch mechanisms are synchronously close or far away.

[0028] The control system starts the electromagnet in the magnetic sliding head and drives the two two-way clutch mechanisms to synchronously move away, so that the clutch block is embedded with the magnetic sliding head. At the same time, the electromagnet in the magnetic sliding head adsorbs the embedded strip. The bottom support supports the clutch block. The two-way clutch mechanism and the magnetic swing mechanism complete the locking. The control system starts the electric telescopic rod. The output shaft of the electric telescopic rod drives the intercepting plate to descend, so that the intercepting plate blocks the overflow channel. Thus, the pretreatment device enters the oscillation mode.

[0029] The control system drives the two bidirectional clutches to synchronously approach, so that the two clutch wheels are embedded in the semicircular grooves on the end block; synchronously, the convex ring is embedded in the groove, avoiding the up-down misplacement sliding of the clutch wheel and the end block, and the bidirectional clutch and the end block assembly are locked; the control system starts the electric telescopic rod, and the output shaft of the electric telescopic rod drives the intercepting plate to rise, so that the overflow channel is completely opened; so that the pre-treatment device enters the centrifugal mode.

[0030] When the pre-treatment device is switched, the electric telescopic column arranged on the host computer is used to temporarily support the supporting piece, and after the mode switching is completed, the control system drives the electric telescopic column to retract.

[0031] Further, the elastic member is a spring, one end of the spring is connected with the magnetic suction sliding head, and the other end of the spring is connected with the sliding seat.

[0032] An algal test method with a pre-treatment function, comprising the following steps:

[0033] S1, the algal sample, glass grinding beads and reagent are put into the double-cavity centrifugal tube;

[0034] S2, the position of the bidirectional clutch is adjusted by using the adjusting mechanism, so that the bidirectional clutch is locked with the magnetic suction swing mechanism, the double-drive mechanism is started, the double-drive mechanism drives the bidirectional clutch to oscillate on the magnetic suction swing mechanism, the bidirectional clutch drives the double-cavity centrifugal tube on the supporting piece to oscillate, so that the glass grinding beads break the wall of the algal sample to obtain a fucoxanthin extract;

[0035] S3, the position of the bidirectional clutch is adjusted by using the adjusting mechanism, so that the bidirectional clutch is locked with the double-drive mechanism, the double-drive mechanism is started, and the double-drive mechanism drives the supporting piece to rotate through the bidirectional clutch, and the supporting piece centrifugally separates the extract in the double-cavity centrifugal tube;

[0036] S4, the separated extract is filtered and then delivered to the detection module in the test host, and the detection module measures the content of fucoxanthin in the extract;

[0037] S5, the double-cavity centrifugal tube is injected with a cleaning liquid, the bidirectional clutch is locked with the magnetic suction swing mechanism again by using the adjusting mechanism, and the double-cavity centrifugal tube on the supporting piece is driven to oscillate by the double-drive structure, so that the cleaning of the double-cavity centrifugal tube is completed.

[0038] Compared with the prior art, the beneficial effects of the present application are:

[0039] 1. The feeding, oscillation breaking extraction, centrifugal separation, flow guiding filtration and detection output are sequentially completed in the same test host, manual transfer and multi-device switching are reduced, the production steps are reduced, the batch detection efficiency and result consistency are improved, and the integration of the processing process is improved.

[0040] 2. The displacement control of the two-way clutch mechanism by the blending mechanism enables the same pre-treatment device to realize quick and reliable switching between the oscillation mode and the centrifugal mode without changing the power source or the carrier, thereby connecting the cell wall breaking and extraction with the centrifugal separation into a continuous controllable process.

[0041] 3. In the oscillation mode, the end assembly drives the two-way clutch mechanism to reciprocate, thereby driving the carrier and the mixed solution in the double-cavity centrifugal tube to reciprocate and oscillate, so that the glass grinding beads break the cell wall of algae and promote the release of fucoxanthin and the mixing of the solution, thereby improving the extraction efficiency.

[0042] 4. In the centrifugal mode, the overflow channel is opened, and the extract is introduced into the centrifugal cavity under the centrifugal action; the glass grinding beads and other solid objects are preferentially retained in the cell wall breaking cavity; a small amount of solid objects entering the overflow channel with the liquid fall back to the cell wall breaking cavity after the centrifugation is completed under the blocking action of the overflow channel inclined structure and the filter plate, thereby reducing the probability of the solid objects entering the centrifugal cavity and the downstream filtration and detection module, and avoiding blockage and pollution.

[0043] 5. After the extraction is completed, the cleaning module injects cleaning liquid into the first and second connecting ports, and adjusts the device to the oscillation mode to clean the inside of the double-cavity centrifugal tube; the cleaning liquid is discharged into the waste liquid collection tank through the first and second discharge ports, and the filter screen of the first discharge port intercepts the cleaned glass grinding beads, so that the glass grinding beads can be recycled without being taken out, thereby reducing the consumption and maintenance costs and improving the continuous operation capacity. BRIEF DESCRIPTION OF DRAWINGS

[0044] Figure 1 It is the overall elevation view of the test equipment of the present application;

[0045] Figure 2 It is the elevation view of the pre-treatment device of the present application Figure One ;

[0046] Figure 3 It is the cross-sectional view of the pre-treatment device of the present application;

[0047] Figure 4 It is the elevation view of the pre-treatment device of the present application Figure Two ;

[0048] Figure 5 It is the elevation view of the magnetic swing mechanism and the two-way clutch mechanism of the present application;

[0049] Figure 6 It is the elevation view of the blending mechanism of the present application;

[0050] Figure 7 It is the elevation view of the carrier of the present application;

[0051] Figure 8This is an elevation view of the dual-chamber centrifuge tube of the present invention;

[0052] Figure 9 For the present invention Figure 8 A magnified view of a portion of region A in the middle;

[0053] Figure 10 This is a cross-sectional view of the support component of the present invention;

[0054] Figure 11 This is an elevation view of the end assembly and bidirectional clutch mechanism of the present invention.

[0055] In the diagram: 1. Test host; 2. Pre-treatment device; 21. Support component; 22. Dual-chamber centrifuge tube; 23. Magnetic swing mechanism; 24. Dual-drive mechanism; 25. Mixing mechanism; 26. Bidirectional clutch mechanism; 211. Limiting slide groove; 212. Collection tank; 213. Cleaning tank; 221. Breaking chamber; 222. Centrifuge chamber; 223. First end cap; 224. Connecting component; 225. Electric telescopic rod; 226. Overflow channel; 227. Interception plate; 228. Filter plate; 229. Second end cap; 2211. First discharge port; 2221. Second discharge port; 2 231. First connection port; 2291. Second connection port; 231. Magnetic slider; 232. Slide rod; 233. Slide base; 234. Spring; 2311. Fitting groove; 2312. Base support; 241. Motor; 242. End assembly; 2421. End block; 2422. Drive wheel; 2423. Concave ring; 2424. Semicircular groove; 2425. Groove; 251. Cylinder; 252. Adjustment plate; 253. Bend groove; 261. Slider; 262. Slide column; 263. Clutch block; 264. Fitting strip; 265. Clutch wheel; 266. Protruding ring. Detailed Implementation

[0056] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0057] Example: Figures 1-11 As shown, the present invention provides a technical solution: an algae testing device with pretreatment function includes a testing host 1 and a pretreatment device 2. The testing host 1 is equipped with a detection module, a cleaning module, and an electric lifting device.

[0058] The front treatment device 2 comprises a double drive mechanism 24, a supporting piece 21 and two sets of magnetic attraction swing mechanisms 23, the double drive mechanism 24 is installed on the test host 1, the bottom end of the supporting piece 21 is provided with a blending mechanism 25, the bottom end of the supporting piece 21 is symmetrically and slidingly provided with a two-way clutch mechanism 26, the two-way clutch mechanism 26 is movably connected with the blending mechanism 25, a plurality of double-cavity centrifugal tubes 22 are installed on the supporting piece 21, and the magnetic attraction swing mechanisms 23 are symmetrically installed on the test host 1.

[0059] The test host 1 is provided with a detection module for detecting the content of fucoxanthin in the extraction liquid.

[0060] The double-cavity centrifugal tube 22 comprises a tube body itself, the tube body itself is installed on the supporting piece 21, an end cover assembly is installed on the tube body itself, a connecting piece 224 is installed on the end cover assembly, and a switch assembly is arranged on the connecting piece 224; the double drive mechanism 24 comprises a motor 241, the motor 241 is installed on the test host 1, an end head assembly 242 is installed on the output shaft of the motor 241; the magnetic attraction swing mechanism 23 comprises a sliding seat 233, the sliding seat 233 is installed on the test host 1, a magnetic attraction sliding head 231 is slidingly installed on the sliding seat 233, and a sliding assembly is installed between the magnetic attraction sliding head 231 and the sliding seat 233.

[0061] The two-way clutch mechanism 26 comprises a moving assembly, the moving assembly is slidingly installed at the bottom end of the supporting piece 21, and a clutch assembly is installed on the moving assembly; the sliding assembly comprises a sliding rod 232, the sliding rod 232 is connected with the magnetic attraction sliding head 231, the sliding rod 232 is slidingly connected with the sliding seat 233, and an elastic piece is installed between the magnetic attraction sliding head 231 and the sliding seat 233.

[0062] The elastic piece is a spring 234, one end of the spring 234 is connected with the magnetic attraction sliding head 231, and the other end of the spring 234 is connected with the sliding seat 233.

[0063] The supporting piece 21 is provided with a plurality of limiting sliding grooves 211, the two-way clutch mechanism 26 is slidingly installed in the limiting sliding grooves 211, a plurality of collection grooves 212 are circumferentially arranged on the supporting piece 21, a plurality of cleaning grooves 213 are circumferentially arranged on the supporting piece 21, the cleaning grooves 213 are located on the inner side of the collection grooves 212, and the number of the collection grooves 212 is consistent with that of the cleaning grooves 213.

[0064] The pipe body is respectively provided with a wall-breaking cavity 221, a centrifugal cavity 222 and an overflow channel 226. The wall-breaking cavity 221 and the centrifugal cavity 222 are communicated through the overflow channel 226. The end of the overflow channel 226 communicated with the wall-breaking cavity 221 is lower than the end communicated with the centrifugal cavity 222 in the horizontal height. The overflow channel 226 is provided with a filter plate 228. The bottom end of the wall-breaking cavity 221 is provided with a first discharge port 2211. The first discharge port 2211 is provided with a first valve and a filter screen. The bottom end of the centrifugal cavity 222 is provided with a second discharge port 2221. The second discharge port 2221 is provided with a second valve. The first discharge port 2211 is aligned with the cleaning groove 213, and the second discharge port 2221 is aligned with the collecting groove 212.

[0065] The end cover assembly comprises a first end cover 223 and a second end cover 229. The first end cover 223 is installed on the wall-breaking cavity 221, and the second end cover 229 is installed on the centrifugal cavity 222. The first end cover 223 is provided with a first connecting port 2231, and the second end cover 229 is provided with a second connecting port 2291. The connecting piece 224 is connected with the first end cover 223 and the second end cover 229 at two ends.

[0066] The switch assembly comprises an electric telescopic rod 225. The electric telescopic rod 225 is installed on the connecting piece 224. The output shaft of the electric telescopic rod 225 is installed with an intercepting plate 227. The intercepting plate 227 is slidably connected with the overflow channel 226.

[0067] The moving assembly comprises a sliding block 261. The sliding block 261 is slidably installed in the limiting sliding groove 211. The sliding block 261 is installed with a sliding column 262. The sliding column 262 is movably connected with the blending mechanism 25.

[0068] The clutch assembly comprises a clutch block 263. The clutch block 263 is provided with an embedded strip 264. The clutch block 263 is connected with the sliding column 262. The clutch block 263 is rotatably installed with a clutch wheel 265. The clutch wheel 265 is provided with a convex ring 266.

[0069] The blending mechanism 25 comprises a cylinder 251 and a blending disc 252. The cylinder 251 is rotatably installed at the bottom end of the supporting piece 21. The blending disc 252 is rotatably installed at the bottom end of the supporting piece 21. The output shaft of the cylinder 251 is rotatably connected with the blending disc 252. The blending disc 252 is provided with a curved groove 253. The sliding column 262 is movably connected with the curved groove 253.

[0070] The magnetic attraction sliding head 231 is provided with an electromagnet. The magnetic attraction sliding head 231 is provided with an embedded groove 2311. The embedded groove 2311 is used for mutually embedding the embedded strip 264. The embedded strip 264 is made of an adsorbable material. The bottom end of the magnetic attraction sliding head 231 is provided with a bottom support 2312.

[0071] The end assembly 242 comprises an end block 2421, and half-circle grooves 2424 are arranged on both sides of the end block 2421, recesses 2425 are arranged in the half-circle grooves 2424, a driving wheel 2422 is rotatably arranged on the end block 2421, a recess ring 2423 is arranged on the driving wheel 2422, the recess ring 2423 can be embedded with the convex ring 266, the half-circle grooves 2424 can be embedded with the clutch wheel 265, and the recesses 2425 can be embedded with the convex ring 266.

[0072] An algal test method with a pre-treatment function, comprising the following steps:

[0073] S1, the algal sample, glass grinding beads and reagents are put into the double-cavity centrifugal tube 22;

[0074] S2, the position of the bidirectional clutch mechanism 26 is adjusted by the blending mechanism 25, the bidirectional clutch mechanism 26 is locked with the magnetic attraction swing mechanism 23, the double-drive mechanism 24 is started, the double-drive mechanism 24 drives the bidirectional clutch mechanism 26 to oscillate on the magnetic attraction swing mechanism 23, the bidirectional clutch mechanism 26 drives the double-cavity centrifugal tube 22 on the support 21 to oscillate, so that the glass grinding beads break the wall of the algal sample, and a fucoxanthin extract is obtained;

[0075] S3, the position of the bidirectional clutch mechanism 26 is adjusted by the blending mechanism 25, the bidirectional clutch mechanism 26 is locked with the double-drive mechanism 24, the double-drive mechanism 24 is started, the double-drive mechanism 24 drives the support 21 to rotate through the bidirectional clutch mechanism 26, and the support 21 centrifugally separates the extract in the double-cavity centrifugal tube 22;

[0076] S4, the separated extract is filtered and then delivered to a detection module in the test host 1, and the detection module measures the content of fucoxanthin in the extract;

[0077] S5, the double-cavity centrifugal tube 22 is injected with a cleaning liquid, the bidirectional clutch mechanism 26 is locked with the magnetic attraction swing mechanism 23 again by the blending mechanism 25, and the double-cavity centrifugal tube 22 on the support 21 is driven to oscillate by the double-drive structure, so that the double-cavity centrifugal tube 22 is cleaned.

[0078] The working principle of the present application is as follows: the test host 1 is provided with a feeding module, before testing, the first valve and the second valve are in a closed state, the overflow channel 226 is intercepted as a whole by the intercepting plate 227, so that the wall-breaking cavity 221 and the centrifugal cavity 222 are separated. The control system respectively inputs algal samples, anhydrous ethanol, glass grinding beads and other medicines and reagents into the first connecting port 2231 in proportion through the feeding module, and the mixed solution enters the wall-breaking cavity 221 from the first connecting port 2231.

[0079] The control system opens the cylinder 251, which drives the mixing disc 252 to deflect at the bottom end of the support 21 through the output shaft. The mixing disc 252 drives the slide column 262 to move through the curved groove 253. Since the slider 261 connected with the slide column 262 can only slide linearly in the limiting sliding groove 211, the entire bidirectional clutch mechanism 26 can only reciprocate along the limiting sliding groove 211 under the driving action of the mixing disc 252, and the two bidirectional clutch mechanisms 26 are synchronized to approach or move away. The two bidirectional clutch mechanisms 26 are synchronized to approach and embed with the end assembly 242, realizing the connection with the double-drive mechanism 24; the two bidirectional clutch mechanisms 26 are synchronized to move away, realizing the connection with the magnetic attraction swing mechanism 23.

[0080] The control system opens the electromagnet in the magnetic attraction slide head 231 and drives the two bidirectional clutch mechanisms 26 to move away synchronously, so that the clutch block 263 is embedded with the magnetic attraction slide head 231, and the electromagnet in the magnetic attraction slide head 231 adsorbs the embedded strip 264, the bottom support 2312 supports the clutch block 263, and the bidirectional clutch mechanism 26 and the magnetic attraction swing mechanism 23 are locked; the control system opens the electric telescopic rod 225, the output shaft of the electric telescopic rod 225 drives the intercepting plate 227 to descend, so that the intercepting plate 227 blocks the overflow channel 226; so that the front treatment device 2 enters the oscillation mode.

[0081] The control system drives the two bidirectional clutch mechanisms 26 to approach synchronously, so that the two clutch wheels 265 are embedded with the semicircular groove 2424 on the end block 2421; synchronously, the convex ring 266 is embedded with the groove 2425, avoiding the up-down misalignment of the clutch wheel 265 and the end block 2421, and the bidirectional clutch mechanism 26 and the end assembly 242 are locked; the control system opens the electric telescopic rod 225, the output shaft of the electric telescopic rod 225 drives the intercepting plate 227 to ascend, so that the overflow channel 226 is completely opened; so that the front treatment device 2 enters the centrifugal mode.

[0082] When the front treatment device 2 switches modes, the electric telescopic column set on the test host 1 temporarily supports the support 21, and after the mode switching is completed, the control system drives the electric telescopic column to retract.

[0083] After the mixed solution enters the broken wall cavity 221, the control system makes the pretreatment device 2 enter the oscillation mode through the mixing mechanism 25, and then the control system starts the motor 241, the output shaft of the motor 241 drives the end head assembly 242 to rotate, and when the driving wheel 2422 on the end head assembly 242 rotates to the position of the clutch wheel 265, the clutch wheel 265 is extruded, and after the clutch wheel 265 is extruded, the magnetic attraction sliding head 231 locked with the clutch wheel 265 slides on the sliding seat 233, the magnetic attraction sliding head 231 extrudes the spring 234 and drives the sliding rod 232 to slide stably on the sliding seat 233, and when the driving wheel 2422 rotates away from the position of the clutch wheel 265, the pressure on the clutch wheel 265 disappears, and the spring 234 drives the bidirectional clutch mechanism 26 to slide reversely through the magnetic attraction sliding head 231, so that the bidirectional clutch mechanism drives the support 21 to slide reciprocatingly, and the support 21 drives the mixed solution in the plurality of double-cavity centrifugal tubes 22 to oscillate reciprocatingly, in the oscillation process, the glass grinding beads break the cell wall of the algal sample, so that the fucoxanthin is released from the cell wall, and the released fucoxanthin is mixed with the solution, so that the preliminary extraction of the fucoxanthin is realized.

[0084] After the preliminary extraction of the fucoxanthin is completed, the control system switches the pretreatment device 2 to the centrifugal mode, and starts the motor 241, the output shaft of the motor 241 drives the end head assembly 242, the end head assembly 242 drives the bidirectional clutch mechanism 26 locked therewith to rotate rapidly, the bidirectional clutch mechanism 26 drives the support 21 to rotate, and the support 21 drives the plurality of double-cavity centrifugal tubes 22 to rotate, under the centrifugal action, the fucoxanthin extract is thrown from the broken wall cavity 221 into the overflow channel 226, enters the centrifugal cavity 222 after passing through the filter plate 228; the glass grinding beads and other solid materials with heavy quality are retained in the broken wall cavity 221, and a small part of the solid materials enter the overflow channel 226 together with the extract, and after the centrifugal ends, the solid materials fall back into the broken wall cavity 221 under the cooperation of the upward structure design of the overflow channel 226 and the blocking effect of the filter plate 228, so that the centrifugal separation of the extract is completed, and the solid materials in the mixed solution are prevented from entering the centrifugal cavity 222.

[0085] After the centrifugal ends, the control system starts the second discharge port 2221, the extract passes through the second discharge port 2221 and the collection groove 212, enters the flow guide mechanism in the test host 1, the flow guide mechanism filters the extract through the organic filter membrane, and then delivers the extract to the detection module for testing, so that the full-automatic extraction and testing of the fucoxanthin are completed.

[0086] The test host 1 is internally provided with a cleaning module, when the extract is discharged, the control system injects cleaning liquid into the first connecting port 2231 and the second connecting port 2291 through the cleaning module, then adjusts the pretreatment device 2 to the oscillation mode, and the cleaning liquid is cleaned in the double-cavity centrifugal tube 22 under the action of vibration, after cleaning, the control system opens the first valve and the second valve, the cleaning liquid falls from the first discharge port 2211 and the second discharge port 2221, respectively flows into the waste liquid collecting box in the cleaning module through the collecting groove 212 and the cleaning groove 213, so as to complete the cleaning of the double-cavity centrifugal tube 22; at the same time, the filter screen arranged on the first discharge port 2211 prevents the glass beads from falling, so that the glass beads can be retained, and the cleaning liquid also cleans the glass beads while cleaning the double-cavity centrifugal tube 22, so that the glass beads can be recycled without being taken out for cleaning, and the batch test efficiency is improved.

[0087] It will be apparent to those skilled in the art that the application is not limited to the details of the above-exemplified embodiments and that the application can be implemented in other concrete forms without departing from the spirit or essential characteristics of the application. Therefore, the embodiments should be considered in all respects as illustrative and not restrictive, the scope of the application being defined by the appended claims rather than by the above description, and it is intended that all changes which come within the meaning and range of equivalency of the claims are encompassed therein. Any reference signs in the claims should not be construed as limiting the claims concerned.

Claims

1. An algal test apparatus with a pre-treatment function, characterized by: The test equipment includes a test host (1) and a pretreatment device (2), the test host (1) is internally provided with a detection module, a cleaning module and an electric lifting device, the detection module is used for testing algae, and the cleaning module is used for cleaning the pretreatment device (2); The pretreatment device (2) includes a double-drive mechanism (24), a supporting piece (21) and two groups of magnetic attraction swing mechanisms (23), the double-drive mechanism (24) is installed on the test host (1), the bottom end of the supporting piece (21) is provided with an adjusting mechanism (25), the bottom end of the supporting piece (21) is symmetrically and slidably provided with a two-way clutch mechanism (26), the two-way clutch mechanism (26) is movably connected with the adjusting mechanism (25), a plurality of double-cavity centrifugal tubes (22) are installed on the supporting piece (21), the magnetic attraction swing mechanisms (23) are symmetrically installed on the test host (1), and the adjusting mechanism (25) is used for controlling and adjusting the disconnection of the two groups of two-way clutch mechanisms (26) and the magnetic attraction swing mechanisms (23) and the double-drive mechanism (24); The double-cavity centrifugal tube (22) includes a tube body itself, the tube body itself is installed on the supporting piece (21), an end cover assembly is installed on the tube body itself, a connecting piece (224) is installed on the end cover assembly, and a switch assembly is arranged on the connecting piece (224); The double-drive mechanism (24) includes a motor (241), the motor (241) is installed on the test host (1), and an end head assembly (242) is installed on the output shaft of the motor (241); The magnetic attraction swing mechanism (23) includes a sliding seat (233), the sliding seat (233) is installed on the test host (1), a magnetic attraction sliding head (231) is slidably installed on the sliding seat (233), and a sliding assembly is installed between the magnetic attraction sliding head (231) and the sliding seat (233); The two-way clutch mechanism (26) includes a moving assembly, the moving assembly is slidably installed at the bottom end of the supporting piece (21), and a clutch assembly is installed on the moving assembly; The sliding assembly includes a sliding rod (232), the sliding rod (232) is connected with the magnetic attraction sliding head (231), the sliding rod (232) is slidably connected with the sliding seat (233), and an elastic piece is installed between the magnetic attraction sliding head (231) and the sliding seat (233); A plurality of limiting sliding grooves (211) are arranged on the supporting piece (21), the two-way clutch mechanism (26) is slidably installed in the limiting sliding groove (211), a plurality of collection grooves (212) are annularly arranged on the supporting piece (21), a plurality of cleaning grooves (213) are annularly arranged on the supporting piece (21), the cleaning grooves (213) are located on the inner side of the collection grooves (212), and the number of the collection grooves (212) is consistent with that of the cleaning grooves (213); The pipe body is respectively provided with a wall-breaking cavity (221), a centrifugal cavity (222) and an overflow channel (226) therein, the wall-breaking cavity (221) and the centrifugal cavity (222) are communicated through the overflow channel (226), the end of the overflow channel (226) communicated with the wall-breaking cavity (221) is lower than the end communicated with the centrifugal cavity (222) in horizontal height, the overflow channel (226) is provided with a filter plate (228), the bottom end of the wall-breaking cavity (221) is provided with a first discharge port (2211), the first discharge port (2211) is provided with a first valve, the first discharge port (2211) is also provided with a filter screen, the bottom end of the centrifugal cavity (222) is provided with a second discharge port (2221), the second discharge port (2221) is provided with a second valve, the first discharge port (2211) is aligned with the cleaning groove (213), and the second discharge port (2221) is aligned with the collecting groove (212); The moving assembly comprises a sliding block (261) slidingly installed in a limiting sliding groove (211), and a sliding column (262) is installed on the sliding block (261); and the sliding column (262) is movably connected with the blending mechanism (25). The clutch assembly comprises a clutch block (263), the clutch block (263) is provided with an embedded strip (264), the clutch block (263) is connected with the connecting piece (224), and a clutch wheel (265) is rotatably installed on the clutch block (263) and provided with a convex ring (266). The blending mechanism (25) comprises a gas cylinder (251) and a blending disc (252), one end of the gas cylinder (251) is rotatably installed at the bottom end of the supporting piece (21), and the blending disc (252) is rotatably installed at the bottom end of the supporting disc; the output shaft of the gas cylinder (251) is rotatably connected with the blending disc (252), the blending disc (252) is provided with a curved groove (253), and the sliding column (262) is movably connected with the curved groove (253).

2. An algal test apparatus with a pre-treatment function according to claim 1, characterized in that: The end cover assembly comprises a first end cover (223) and a second end cover (229), the first end cover (223) is installed on the wall-breaking cavity (221), the second end cover (229) is installed on the centrifugal cavity (222), the first end cover (223) is provided with a first connecting port (2231), the second end cover (229) is provided with a second connecting port (2291), and the connecting piece (224) is connected with the first end cover (223) and the second end cover (229) at two ends respectively; The switch assembly comprises an electric telescopic rod (225), the electric telescopic rod (225) is installed on the connecting piece (224), an intercepting plate (227) is installed on the output shaft of the electric telescopic rod (225), and the intercepting plate (227) is slidably connected with the overflow channel (226).

3. The algal test apparatus with a pre-treatment function according to claim 1, characterized by: The magnetic attraction sliding head (231) is internally provided with an electromagnet, the magnetic attraction sliding head (231) is provided with an embedded groove (2311), the embedded groove (2311) is used for being embedded with an embedded strip (264), the embedded strip (264) is made of an adsorbable material, and the magnetic attraction sliding head (231) is provided with a bottom support (2312) at the bottom end. The end head assembly (242) comprises an end head block (2421), both sides of the end head block (2421) are provided with a semicircular groove (2424), the semicircular groove (2424) is internally provided with a groove (2425), the end head block (2421) is rotatably provided with a driving wheel (2422), the driving wheel (2422) is provided with a concave ring (2423), the concave ring (2423) can be embedded with a convex ring (266), the semicircular groove (2424) can be embedded with a clutch wheel (265), and the groove (2425) can be embedded with the convex ring (266).

4. The algal test apparatus with a pre-treatment function according to claim 1, characterized by: The elastic member is a spring (234), one end of the spring (234) is connected with the magnetic attraction sliding head (231), and the other end of the spring (234) is connected with the sliding seat (233).

5. An algal test method with a pre-treatment function, characterized by: The algal test method comprises the following steps: S1, the algal sample, glass grinding beads and extraction reagents are put into the double-cavity centrifugal tube (22); S2, the position of the bidirectional clutch mechanism (26) is adjusted by the adjusting mechanism (25), the bidirectional clutch mechanism (26) is locked with the magnetic attraction swinging mechanism (23), the double-drive mechanism (24) is started, the double-drive mechanism (24) drives the bidirectional clutch mechanism (26) to oscillate on the magnetic attraction swinging mechanism (23), the bidirectional clutch mechanism (26) drives the double-cavity centrifugal tube (22) on the supporting piece (21) to oscillate, and the glass grinding beads break the wall of the algal sample to obtain a fucoxanthin extract; S3, the position of the bidirectional clutch mechanism (26) is adjusted by the adjusting mechanism (25), the bidirectional clutch mechanism (26) is locked with the double-drive mechanism (24), the double-drive mechanism (24) is started, the double-drive mechanism (24) drives the supporting piece (21) to rotate through the bidirectional clutch mechanism (26), and the supporting piece (21) centrifugally separates the extract in the double-cavity centrifugal tube (22); S4, the separated extract is filtered and then conveyed to a detection module in the test host (1), and the detection module measures the content of the fucoxanthin in the extract; S5, the double-cavity centrifugal tube (22) is injected with a cleaning liquid, the bidirectional clutch mechanism (26) is locked with the magnetic attraction swinging mechanism (23) again by the adjusting mechanism (25), the double-cavity centrifugal tube (22) on the supporting piece (21) is oscillated through the double-drive structure, and the double-cavity centrifugal tube (22) is cleaned.

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