Multi-wavelength continuous scanning circular dichroism spectrum testing system and method

Abstract

The invention provides a multi-wavelength continuous scanning circular dichroism spectrum testing system. The multi-wavelength continuous scanning circular dichroism spectrum testing system comprises a plurality of synchrotron radiation light sources, a polarizer, a photoelectric modulator, a micro-fluidic device and a light path channel, the synchrotron radiation light source is fixed in the fixed hollow cavity, a plurality of mirror surfaces are correspondingly arranged on one side of the light source, and the mirror surfaces are used for separating emitted waves of the synchrotron radiation light source in the light path channel; the synchrotron radiation light source is connected with and can rotate the polarizer, the photoelectric modulator and the micro-fluidic device, and emergent waves are sequentially sent to the polarizer, the photoelectric modulator and the micro-fluidic device through the light path channel; the synchrotron radiation light source is also connected with and can rotate a polarizer and a microfluidic device, and emergent waves are sequentially sent to the polarizer and the microfluidic device through a light path channel; the system further comprises a plurality of detectors and a multi-wavelength monochromatic light source, wherein the detectors are used for detecting emitted waves passing through the micro-fluidic device. The system can realize continuous flow and composite monochromatic light scanning and qualitative and quantitative analysis at the same time.

Description

Technical Field

[0001] This invention relates to the field of polarization spectroscopy, specifically to a multi-wavelength continuous scanning circular dichroism spectroscopy testing system and method. Background Technology

[0002] Circular dichroism (CD) spectroscopy is defined as the difference in absorption of left-handed and right-handed circularly polarized light by a sample. This spectroscopic technique is widely used in the study of chiral molecules. In existing technologies, synchrotron radiation circular dichroism (CD) differs from conventional CD in that it extends into the vacuum ultraviolet band and utilizes the high-intensity ultraviolet and vacuum ultraviolet light sources provided by synchrotron radiation. It is suitable for characterizing the chiral features of sugars, proteins, and nucleic acids below 200 nm wavelengths, such as resolving the short-wavelength Cotton peaks corresponding to structures like α-helices, β-sheets, and γ-turns. Conventional CD, on the other hand, is limited to wavelengths above 200 nm and cannot scan and resolve Cotton peaks below 200 nm. However, synchrotron radiation circular dichroism (CDD) is mainly used to resolve the chiral characteristics of sugars, proteins, and nucleic acids below 200 nm wavelength, and to identify the optical rotation properties of qualitative chiral structures by comparing with a database. In contrast, conventional CDD can be widely used to generate characteristic peaks in specific wavelength ranges, such as 500-800 nm, based on the chiral characteristics of sugars, proteins, and nucleic acids, to create sensors for sugars, proteins, and nucleic acids. These sensors have higher sensitivity and repeatability and can be used for quantitative analysis of sugars, proteins, and nucleic acids.

[0003] When characterizing the structure of a sample using circular dichroism spectroscopy, a single-scan, short-wavelength monochromatogram is still used, with the sample employing a stop-flow technique. However, this technique is limited to single-wavelength testing and has a relatively long stop time. Continuous flow detection, on the other hand, refers to the different spatial locations corresponding to different moments in the reaction during the continuous flow of the solution after the reaction. Therefore, time-resolved detection is converted into spatial-resolved detection, which has a shorter stop time and can utilize steady-state detection methods to detect the dynamic spectrum across the entire wavelength range. However, traditional continuous flow requires a large amount of sample consumption. Summary of the Invention

[0004] To address the technical problems encountered in existing circular dichroism spectroscopy detection methods, this invention provides a multi-wavelength continuous scanning circular dichroism spectroscopy testing system and method.

[0005] In a first aspect, the present invention provides a multi-wavelength continuous scanning circular dichroism spectroscopy testing system, including a synchrotron radiation source, a polarizer, a photoelectric modulator, a microfluidic device, and an optical path channel;

[0006] The synchrotron radiation source is fixed in a fixed hollow cavity, and there are several mirrors on one side of the source. The mirrors are used to separate the emitted waves of the synchrotron radiation source in the optical path channel.

[0007] The synchrotron radiation source is connected to the polarizer, photoelectric modulator, and microfluidic device. The emitted wave is sent sequentially to the polarizer, photoelectric modulator, and microfluidic device through the optical path channel. The angle between the polarizer, photoelectric modulator, and microfluidic device and the emitted wave is rotated to continuously scan and detect the sample in the microfluidic device and obtain CD signals for qualitative analysis of chiral structure.

[0008] The synchrotron radiation source is connected to the polarizer and the microfluidic device. The emitted wave is sent to the polarizer and the microfluidic device in sequence through the optical path channel. The angle between the polarizer, the microfluidic device and the emitted wave is rotated to continuously scan and detect the sample in the microfluidic device, and to obtain CD signals for quantitative analysis of sample content indicators.

[0009] Furthermore, the microfluidic device includes a microfluidic chip and a surface ion polaron resonant cavity;

[0010] The synchrotron radiation source sends the emitted wave through the optical path channel to the polarizer, photoelectric modulator and microfluidic chip in sequence, for continuous scanning and detection of the sample in the microfluidic device, and to obtain CD signal for qualitative analysis of chiral structure.

[0011] The synchrotron radiation source sends the emitted wave sequentially to the polarizer and the surface ion polariton resonant cavity through the optical path channel for continuous scanning and detection of the sample in the microfluidic device, and obtains CD signal to quantitatively analyze the sample content index.

[0012] Furthermore, the microfluidic device also includes a lower component and an upper component, both of which are provided with a serpentine microfluidic channel region and a surface ion polariton resonant cavity region. The lower component and the upper component are pressed and fixed together, and the microfluidic chip and the surface ion polariton resonant cavity are simultaneously formed in the serpentine microfluidic channel region and the surface ion polariton resonant cavity region.

[0013] Furthermore, the microfluidic device also includes: an inlet channel and an outlet channel, the inlet channel connecting the microfluidic chip and the surface ion polariton resonant cavity, and the diameter of the inlet channel of the surface ion polariton resonant cavity being more than 5 times that of the inlet channel of the microfluidic chip;

[0014] Different liquids are mixed through the inlet pipe, and the mixing area is connected to the inlet pipe of the microfluidic chip.

[0015] Furthermore, the microfluidic chip forms a three-sided serpentine channel through at least two upper component bumps arranged at adjacent intervals and a lower component bump embedded in the upper component groove, namely the bottom of the upper component bump and the three-sided serpentine channel on the left and right sides.

[0016] The three serpentine channels simultaneously converge and connect the liquid outlet channel and the synchronous radiation scanning chamber formed by pressing the upper side wall and the lower side wall of the scanning chamber together;

[0017] The synchrotron radiation scanning chamber has narrow regions on the left and right, and a wide region in the middle. The diameter gradually converges from the wide region in the middle to the narrow regions on both sides. The liquid outlet on the left side is connected to the three-sided serpentine channel, and the liquid outlet channel is connected to the right side.

[0018] Furthermore, the surface ion exciton resonant cavity includes an upper resonant cavity and a lower resonant cavity, wherein the upper resonant cavity is provided with a metal bump for the upper resonant cavity component and a groove for the upper resonant cavity component, and wherein the lower resonant cavity is provided with a metal bump for the lower resonant cavity component and a groove for the lower resonant cavity component.

[0019] The upper component metal bumps of the resonant cavity are evenly spaced and distributed in the upper resonant cavity, forming grooves in the spaced areas; the lower component metal bumps of the resonant cavity are evenly spaced and distributed in the lower resonant cavity, forming grooves in the spaced areas.

[0020] The metal bumps of the upper component of the resonant cavity correspond to the grooves of the lower component of the resonant cavity in the vertical direction, and do not abut against each other; the metal bumps of the lower component of the resonant cavity correspond to the grooves of the upper component of the resonant cavity in the vertical direction, and do not abut against each other.

[0021] In the surface ion polariton resonant cavity, the upper component metal bump and the lower component metal bump of the resonant cavity are integrally etched with the upper component bump and the lower component bump in the microfluidic chip through positive etching or negative etching.

[0022] Furthermore, the lower and upper components are tightly pressed together and sealed and bonded in the first dielectric layer in the consolidation groove by the consolidation protrusions in the consolidation region of the lower and upper components.

[0023] Furthermore, the microfluidic device is encapsulated by a second dielectric layer, and an optical waveguide region is provided on the sidewall of the surface ionotropic resonant cavity region. The surface ionotropic resonant cavity is connected to the first and second optical waveguides of the optical waveguide region, exposing the surface ionotropic resonant cavity and forming an incident window for the linearly polarized light emitted from the polarizer.

[0024] The first optical waveguide is distributed in the lower component, and the second optical waveguide is distributed in the upper component. The first and second optical waveguides are encapsulated by the second dielectric layer to form a structure of optical waveguide / dielectric layer / upper and lower component substrate layers. The upper and lower component substrate layers can be made of silicon dioxide. The optical waveguide, dielectric layer and the upper and lower component substrate layers have a large difference in refractive index, which couples the incident wave into the surface ion polaron resonant cavity to excite surface plasmon waves.

[0025] Furthermore, the multi-wavelength continuous scanning circular dichroism spectroscopy testing system also includes a switch control component and several detectors.

[0026] Furthermore, in some embodiments, the multi-wavelength continuous scanning circular dichroism spectroscopy testing system further includes a multi-wavelength monochromatic light source, which is a long-wavelength monochromatic light source. An optical path intersection area is set, and the composite monochromatic light is mixed with the synchrotron radiation light source through the mirror and sent to the microfluidic device to detect and output a chiral CD signal; the synchrotron radiation light source sends short-wavelength monochromatic light to the microfluidic device to detect and output a chiral CD signal.

[0027] Furthermore, in some other embodiments, the multi-wavelength continuous scanning circular dichroism spectroscopy testing system further includes a multi-wavelength monochromatic light source, a first synchrotron radiation light source, and a second synchrotron radiation light source. The multi-wavelength monochromatic light source emits long-wavelength monochromatic light, which is mixed with short-wavelength monochromatic light emitted by the first synchrotron radiation light source through a mirror and then applied to the surface ion polaron resonant cavity of the microfluidic device for quantitative detection.

[0028] The second synchrotron radiation source emits short-wavelength monochromatic light, which is mixed with the short-wavelength monochromatic light emitted by the first synchrotron radiation source through the mirror and then applied to the microfluidic chip of the microfluidic device for continuous flow scanning to qualitatively detect structures such as α-helices and β-parallels with short wavelengths.

[0029] Secondly, this invention provides a multi-wavelength continuous scanning circular dichroism spectroscopy testing method:

[0030] S100: A shortwave band interval is established. The first synchrotron radiation source generates multi-wavelength monochromatic shortwaves in the predetermined band interval. These shortwaves are then fed into the microfluidic chip of the microfluidic device via a polarizer and a photoelectric modulator. The focusing area C spot is focused. The microfluidic device mixes liquids. The polarizer and photoelectric modulator are rotated to obtain the intensity of the corresponding polarization component of the output beam on the multi-wavelength monochromatic shortwave.

[0031] Based on the continuous scanning results, determine whether to turn on the second synchrotron radiation source. Continuously generate multi-wavelength monochromatic short waves in a predetermined band range according to the short wavelength band range. The first synchrotron radiation source and the second synchrotron radiation source alternately output, continuously capturing until the characteristic wavelength shows positive and negative peaks of Cotton.

[0032] S200: Based on the continuous scanning results, determine whether to adjust the tilt angle of the microfluidic device, and then adjust the incident scanning angle between the microfluidic chip in the microfluidic device and the multi-wavelength monochromatic short wave, continuously capturing until the characteristic wavelength shows positive and negative peaks of Cotton.

[0033] S300: Establish a long-wavelength band range, analyze the characteristic peak distribution range of the sample to be tested, determine whether the multi-wavelength monochromatic light source is combined with the multi-wavelength monochromatic short-wave scanning analysis of the characteristic peaks of the sample to be tested by the first synchrotron radiation light source, generate long waves in the predetermined band range of the multi-wavelength monochromatic light source, and enter the surface ion exciton resonant cavity of the microfluidic device through the polarizer. According to the continuous scanning results, adjust the tilt angle of the microfluidic device through the first synchrotron radiation light source and output the CD signal.

[0034] The beneficial effects achieved by this invention are as follows:

[0035] 1) By connecting the polarizer, photoelectric modulator, and microfluidic device through a synchrotron radiation source, the emitted wave is sequentially sent to the polarizer, photoelectric modulator, and microfluidic device through the optical path channel. By rotating the angle between the polarizer, photoelectric modulator, and emitted wave, continuous scanning and detection of the sample in the microfluidic device can be achieved.

[0036] 2) By setting up a short-wave band interval, the second synchrotron radiation source is activated based on the first continuous scan result. The composite monochromatic short waves of the predetermined band interval are continuously generated according to the short-wave band interval. The first synchrotron radiation source and the second synchrotron radiation source are output alternately and continuously captured until the characteristic wavelength shows the Cotton positive and negative peaks. This enables continuous scanning and detection of samples in microfluidic devices.

[0037] 3) Based on the continuous scanning results, determine whether to adjust the tilt angle of the microfluidic device, and then adjust the incident scanning angle between the microfluidic chip and the composite monochromatic short wave, continuously capture until the characteristic wavelength shows the Cotton positive and negative peaks, and realize multi-angle recognition of the left and right elliptically polarized light generated by the specific angle folding of chiral molecules;

[0038] 4) By combining synchrotron radiation light sources and multi-wavelength monochromatic light sources, the characteristic peak distribution range of the sample under test can be rapidly analyzed across the entire spectrum without consuming a large number of samples and conducting multiple tests. This allows for both qualitative and quantitative rapid analysis of the structure with a single sample.

[0039] 5) By combining synchrotron radiation sources and multi-wavelength monochromatic sources, microfluidic devices are configured with microfluidic chips and surface ion polariton resonant cavities. The synchrotron radiation source emits short waves that enter the microfluidic chip, and trace continuous testing through the microfluidic chip helps to dynamically test the dynamic folding process of macromolecules. The synchrotron radiation source and multi-wavelength monochromatic source emit composite wavelengths, and the plasma resonance generated by the micro-nano structure of the surface ion polariton resonant cavity produces photoactivity and improves the intensity of the CD signal.

[0040] 6) The tilt angle of the microfluidic device is adjusted by a synchrotron radiation source, while a polarizer generates linearly polarized light with symmetrical positive and negative angle values. The changes in the CD signal of chirality analysis are tested from multiple angles to quantitatively analyze the content index.

[0041] 7) The three-sided serpentine structure of the mixer microfluidic chip can fully mix trace amounts of the sample to be tested, and can form laminar flow in the synchrotron radiation scanning chamber to meet the requirements of spectral detection of highly viscous mixtures. Attached Figure Description

[0042] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0043] Figure 1 This is a schematic diagram of the multi-wavelength continuous scanning circular dichroism spectroscopy testing system of this embodiment;

[0044] Figure 2 This is a schematic diagram of another embodiment of a multi-wavelength continuous scanning circular dichroism spectroscopy testing system;

[0045] Figure 3 This is a schematic diagram of another embodiment of a multi-wavelength continuous scanning circular dichroism spectroscopy testing system;

[0046] Figure 4 This is a schematic diagram of another embodiment of a multi-wavelength continuous scanning circular dichroism spectroscopy testing system;

[0047] Figure 5 This is a cross-sectional view of the microfluidic device in this embodiment;

[0048] Figure 6 This is a top cross-sectional view of the microfluidic chip of the microfluidic device in this embodiment;

[0049] Figure 7 This is a front view of the resonant cavity of the microfluidic device in this embodiment;

[0050] Figure 8 This is another schematic diagram of the microfluidic device in this embodiment;

[0051] Figure 9 This is a schematic diagram of the microfluidic device assembly connected to the optical waveguide in this embodiment;

[0052] Figure 10 This is a schematic diagram of the upper and lower components of the microfluidic device in this embodiment.

[0053] Figure 11 This is a schematic diagram of the bonding and assembly of the upper and lower components of the microfluidic device in this embodiment;

[0054] Figure 12 This is a side view of the optical waveguide structure in the microfluidic device in this embodiment;

[0055] Icons: Lower component 10a, Upper component 10b, First liquid inlet channel 11, Second liquid inlet channel 12, Third liquid inlet channel 13, Fourth liquid inlet channel 14, First liquid outlet channel 15, Second liquid outlet channel 16, Upper sidewall of scanning chamber 17a, Lower sidewall of scanning chamber 17b, Synchrotron radiation scanning chamber 18, Third liquid outlet channel 19, Microfluidic device 20, Upper component bump 201a, Upper component groove 201b, Lower component bump 202a, Lower component groove 202b, Upper resonant cavity 203a, Lower resonant cavity 203b, Upper component metal bump 204a, Upper component groove 204b, Lower component metal bump 205a, Lower component groove 205b, Consolidation groove 206a, Consolidation bump 206b, First dielectric layer 207, Second dielectric layer 208, First optical waveguide 209a, Second optical waveguide 209b, ... A test system 100 includes a synchrotron radiation source 101, a polarizer 102, a photoelectric modulator 103, a switch control assembly 104, a mirror 105, a fixed hollow cavity 1001, a first optical path channel 1002, a second optical path channel 1003, a third optical path channel 1004, a fourth optical path channel 1005, a first output optical path channel 1006, and a second output optical path channel 1007. A second test system 200 includes a third test system 300, a synchrotron radiation source 301, a multi-wavelength monochromatic light source 302, a first detector 303, a second detector 304, and a mirror 305. A fourth test system 400 includes a first synchrotron radiation source 401, a multi-wavelength monochromatic light source 402, a second synchrotron radiation source 403, a switch control assembly 404, a mirror 405, an A resonant cavity region, a B serpentine microfluidic channel, a detection focusing region C, a consolidation region D, and an optical waveguide region E. Detailed Implementation

[0056] To better understand the above-mentioned objectives, features, and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0057] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0058] like Figure 1-4 This is a schematic diagram of several structures of a multi-wavelength continuous scanning circular dichroism spectroscopy testing system.

[0059] like Figure 5-12 This is a schematic diagram of the component structure of a multi-wavelength continuous scanning circular dichroism spectroscopy testing system.

[0060] Firstly, a microfluidic chip is provided, such as... Figure 5 and Figure 6 The assembly includes: an upper component protrusion 201a, an upper component groove 201b, and a lower component protrusion 202a and a lower component groove 202b. The upper component protrusion 201a and the upper component groove 201b are arranged alternately to form a serrated arrangement, and the lower component protrusion 202a and the lower component groove 202b are arranged alternately to form a serrated arrangement. The upper component protrusion 201a is perpendicular to the lower component groove 202b and is embedded in the corresponding lower component groove 202b, and the lower component protrusion 202a is perpendicular to the upper component groove 201b and is embedded in the corresponding upper component groove 201b.

[0061] The upper component protrusion 201a is embedded but does not abut against the corresponding lower component groove 202b, and the lower component protrusion 202a is embedded but does not abut against the corresponding upper component groove 201b;

[0062] At least two upper component protrusions 201a arranged at intervals and lower component protrusions 202a embedded in upper component grooves 201b form a three-sided serpentine channel, namely the bottom of upper component protrusions 201a and the left and right sides of the three-sided serpentine channel.

[0063] The three serpentine channels converge and connect the second liquid outlet channel 16 and the synchrotron radiation scanning chamber 18 formed by pressing the upper side wall 17a and the lower side wall 17b of the scanning chamber together.

[0064] The synchrotron radiation scanning chamber 18 is a narrow region around the perimeter and a wide region in the middle. The diameter gradually converges from the wide region in the middle to the narrow regions on both sides. The liquid outlet on the left side is a three-sided serpentine channel, and the third liquid outlet channel 19 is a three-sided serpentine channel on the right side.

[0065] like Figure 11 The first dielectric layer 207 is bonded to the consolidation groove 206a by the consolidation protrusion 206b in the consolidation region D, as shown in the figure. Figure 10 The upper component bump 201a is tightly embedded in the corresponding lower component groove 202b, and the lower component bump 202a is tightly embedded in the corresponding upper component groove 201b, thus obtaining a microfluidic chip.

[0066] In some embodiments, the upper component bump 201a and the lower component bump 202a are made of quartz material, and the upper component bump 201a and the lower component bump 202a of corresponding size specifications are obtained by photolithography. More specifically, by using positive film process, the pattern of the photolithography exposure process is controlled, and after exposure and development, the upper component bump 201a and the lower component bump 202a of corresponding length and width specifications are obtained. By controlling the etching parameters of the photolithography etching process, the upper component bump 201a and the lower component bump 202a of corresponding height specifications are obtained by depth etching. When the upper component bump 201a and the lower component bump 202a are pressed into the upper component groove 201b and the lower component groove 202b, the size of the three-sided serpentine channel is controlled by positive film photolithography process.

[0067] In other embodiments, the upper component bump 201a and the lower component bump 202a are made of calcium fluoride or metal.

[0068] In other embodiments, the upper component bump 201a and the lower component bump 202a are both fabricated using a negative photolithography process to form a groove structure. The groove is injected with photoresist, exposed and developed, and then ion implanted to form the patterns of the upper component bump 201a and the lower component bump 202a. After the photoresist is washed away, the patterns of the upper component groove 201b and the lower component groove 202b are formed.

[0069] Secondly, a surface ion exciton resonant cavity is provided, such as Figure 5 and Figure 7 The resonant cavity 203a and the resonant cavity 203b are provided, wherein the resonant cavity 203a is provided with a metal protrusion 204a and a groove 204b of the upper component of the resonant cavity, and the resonant cavity 203b is provided with a metal protrusion 205a and a groove 205b of the lower component of the resonant cavity.

[0070] The upper resonant cavity 203a and the lower resonant cavity 203b are the same size. The upper resonant cavity 203a and the lower resonant cavity 203b are tightly pressed and sealed by the first dielectric layer 207 in the solidification groove 206a through the solidification protrusion 206b in the solidification region D.

[0071] Metal bumps 204a of the upper component of the resonant cavity are evenly spaced in the upper resonant cavity 203a, forming grooves 204b of the upper component of the resonant cavity in the spaced area; metal bumps 205a of the lower component of the resonant cavity are evenly spaced in the lower resonant cavity 203b, forming grooves 205b of the lower component of the resonant cavity in the spaced area.

[0072] The metal bump 204a of the upper component of the resonant cavity corresponds to the groove 205b of the lower component of the resonant cavity in the vertical direction, and they do not abut against each other; the metal bump 205a of the lower component of the resonant cavity corresponds to the groove 204b of the upper component of the resonant cavity in the vertical direction, and they do not abut against each other.

[0073] In the surface ion polariton resonant cavity, the upper component metal bump 204a and the lower component metal bump 205a of the resonant cavity are integrally etched with the upper component bump 201a and the lower component bump 202a in the microfluidic chip.

[0074] The surface ion exciton resonant cavity formed by the upper resonant cavity 203a and the lower resonant cavity 203b is connected to the first liquid outlet channel 15 on the left and the fourth liquid inlet channel 14 on the right.

[0075] In some embodiments, the upper component metal bump 204a and the lower component metal bump 205a of the resonant cavity are made of noble metal. The upper component metal bump 204a and the lower component metal bump 205a of the resonant cavity can induce collective oscillation of electrons, forming surface plasmon waves, thereby increasing the absorption intensity of the incident wave.

[0076] In other embodiments, the upper component metal bump 204a and the lower component metal bump 205a of the resonant cavity are made of copper-based, nickel-based, or iron-based materials wrapped with graphene layers. A 10-15 nm graphene layer is coated or chemically vapor-deposited on the outer surface of the copper-based, nickel-based, or iron-based materials. The two-dimensional graphene material can provide a tunable electron concentration, and its surface can also generate electron collective oscillations similar to those of metals, forming surface plasmon resonance waves. It can support highly confined surface plasmon resonances (SPP) and has tunable dispersion. Compared with noble metals, it has low heat loss, low electron concentration (compared to metals), and less loss during SPP propagation.

[0077] In some embodiments, the metal bump 204a of the upper component of the resonant cavity and the groove 205b of the lower component of the resonant cavity have the same length and width. The metal bump 205a of the lower component of the resonant cavity and the groove 204b of the upper component of the resonant cavity have the same length and width, with a width of 50-150nm and a height of 30-120nm.

[0078] In some embodiments, the upper component metal bump 204a and the lower component metal bump 205a of the resonant cavity are both fabricated using a negative photolithography process to form a groove structure. The groove is injected with negative photoresist, exposed and developed, and then ion implanted to form the patterns of the upper component metal bump 204a and the lower component metal bump 205a of the resonant cavity. After the photoresist is washed away, the patterns of the lower component groove 205b and the upper component groove 204b of the resonant cavity are formed.

[0079] Thirdly, a microfluidic device is provided, such as... Figure 5It includes: a lower component 10a, an upper component 10b, and a microfluidic chip and a surface ion polariton resonant cavity distributed in the junction area of ​​the lower component 10a and the upper component 10b. The lower component 10a and the upper component 10b are each provided with a serpentine microfluidic channel area B and a surface ion polariton resonant cavity area A. The lower component 10a and the upper component 10b are bonded to the consolidation groove 206a by consolidation bumps 206b. The microfluidic chip and the surface ion polariton resonant cavity are simultaneously formed in the serpentine microfluidic channel area B and the surface ion polariton resonant cavity area A.

[0080] The lower component 10a is also provided with a first liquid inlet channel 11 and a third liquid inlet channel 13, and the upper component 10b is provided with a second liquid inlet channel 12 and a third liquid inlet channel 13. The third liquid inlet channel 13 connects the first liquid inlet channel 11 and the second liquid inlet channel 12. The third liquid inlet channel 13 separates the liquid and connects the fourth liquid inlet channel 14, the second liquid outlet channel 16, the first liquid outlet channel 15 and the third liquid outlet channel 19.

[0081] The diameter of the third liquid inlet channel 13 is more than 5 times the diameter of the third liquid outlet channel 19, enabling the third liquid outlet channel 19 to extract trace amounts of liquid.

[0082] In some embodiments, such as Figure 9 and Figure 12 The second dielectric layer 208 encapsulates the microfluidic device. An optical waveguide region E is provided on the sidewall of the surface ionotropic resonant cavity region A. The surface ionotropic resonant cavity is connected to the first optical waveguide 209a and the second optical waveguide 209b of the optical waveguide region, exposing the surface ionotropic resonant cavity and forming an incident window.

[0083] The first optical waveguide 209a is distributed in the lower component 10a, and the second optical waveguide 209b is distributed in the upper component 10b. The first optical waveguide 209a and the second optical waveguide 209b are encapsulated by the second dielectric layer 208, forming a structure of optical waveguide / dielectric layer / upper and lower component substrate layers. The upper and lower component substrate layers can be made of silicon dioxide. The optical waveguide, dielectric layer and the upper and lower component substrate layers have a large difference in refractive index, which couples the incident wave into the surface ion polariton resonant cavity to excite surface plasmon waves. Combining the surface ion polariton and the optical waveguide, the system is in a plasma-induced transparent resonant state at the critical waveguide thickness. The bonding and antibonding modes have equal losses, and the electric field is uniformly distributed in the metal and waveguide regions. Using the coupling mode can reduce the high loss of the surface plasmon polariton while maintaining its subwavelength characteristics.

[0084] Fourthly, a multi-wavelength continuous scanning circular dichroism spectroscopy testing system is provided, such as... Figure 1 The first test system 100 includes the following components: synchrotron radiation source 101, polarizer 102, photoelectric modulator 103, switch control component 104, mirror 105, microfluidic device 20 and several optical path channels.

[0085] Synchrotron radiation source 101 is fixed in fixed hollow cavity 1001. The opening direction of fixed hollow cavity 1001 is perpendicular to the third optical path channel 100 and corresponds to mirror 105. Mirror 105 is used to split the emitted wave of synchrotron radiation source 101 in third optical path channel 1004.

[0086] The first optical path channel 1002 is connected to the third optical path channel 1004. A mirror 105 and a polarizer 102 are set according to the optical path direction. The mirror 105 of the first optical path channel 1002 sends the emitted wave of the synchrotron radiation source 101 split from the third optical path channel 1004 to the polarizer 102.

[0087] The second optical path channel 1003 connects to the third optical path channel 1004. A mirror 105, a polarizer 102, and a photoelectric modulator 103 are arranged according to the optical path direction. The mirror 105 of the second optical path channel 1003 will generate the emitted wave of the synchrotron radiation source 101 split from the third optical path channel 1004 to the polarizer 102 and the photoelectric modulator 103 in sequence.

[0088] The fourth optical path channel 1005 is set parallel to the third optical path channel 1004, and sends the linearly polarized light of the first optical path channel 1002 and the left and right elliptic polarized light of the second optical path channel 1003 to the first output optical path channel 1006 and the second output optical path channel 1007, respectively.

[0089] The fourth optical path channel 1005 is equipped with a microfluidic device 20. The surface ion exciton resonance of the microfluidic device 20 is placed in the first optical path channel 1002 to receive the linearly polarized light from the first optical path channel 1002 and convert it into left- or right-handed elliptically polarized light, which is then emitted through the first output optical path channel 1006. The microfluidic chip of the microfluidic device 20 is placed in the second optical path channel 1003 to receive the left- or right-handed elliptically polarized light from the second optical path channel 1003 and convert it into left- or right-handed elliptically polarized light with an angle difference, which is then emitted through the second output optical path channel 1007.

[0090] Synchrotron radiation source 101 conducts polarizer 102, photoelectric modulator 103 and microfluidic device 20 through switch control component 104, and rotates the angle between polarizer 102, photoelectric modulator 103 and microfluidic device 20 and the emitted wave of synchrotron radiation source 101.

[0091] The switch control component 104 controls the angle of the mirror 105.

[0092] The synchrotron radiation source 101 rotates the polarizer 102 and the photoelectric modulator 103 through the switch control component 104, so that the synchrotron radiation source 101 can convert the emitted composite monochromatic wave into continuous left and right elliptically polarized light, continuously scan the microfluidic chip of the microfluidic device 20, realize multi-wavelength continuous scanning, and quickly output the CD signal corresponding to the sample under test in the microfluidic chip with different wavelengths.

[0093] Meanwhile, due to the micro-nano structure of the surface ion polariton resonant cavity in the microfluidic device 20, it can contact the emitted composite monochromatic wave from the synchrotron radiation source 101 to generate surface plasmon waves inside or on the surface of the cavity, thereby exciting the photoactivity of the optical field and enhancing the intensity of the CD signal. At the same time, by adjusting the linearly polarized light with different symmetry angles output by the polarizer 102, the change in the chiral signal CD of the sample to be tested in the microfluidic device 20 can be measured, thereby realizing the circular dichroism detection of the sample to be tested. Then, the change in the concentration of the sample to be tested can be inferred from the change in the intensity of the CD signal, thus realizing quantitative analysis.

[0094] In some embodiments, such as Figure 2 The second test system 200 also includes several detectors, which will output CD signals corresponding to left- and right-handed elliptically polarized light. The synchrotron radiation source 101 adjusts the tilt angle of the microfluidic device 20 through the switch control component 104, thereby adjusting the incident scanning angle between the microfluidic chip and the surface ion exciton resonant cavity and the incident wave.

[0095] In other embodiments, such as Figure 3 The third testing system 300 also includes a multi-wavelength monochromatic light source 302, a first detector 303, and a second detector 304. The multi-wavelength monochromatic light source 302 (long-wavelength monochromatic light) is located in the intersection area of ​​the first optical path channel 1002 and the third optical path channel 1004. It mixes the composite monochromatic light with the synchrotron radiation light source 301 (short-wavelength monochromatic light) through the mirror 305 and sends it to the microfluidic device. The first detector 303 detects and outputs a CD signal. The synchrotron radiation light source 301 sends short-wavelength monochromatic light to the microfluidic device, and the second detector 304 detects and outputs a CD signal.

[0096] In other embodiments, such as Figure 4 The fourth testing system 400 also includes a multi-wavelength monochromatic light source 402, a second synchrotron radiation light source 403, and a switch control component 404. The multi-wavelength monochromatic light source 402 emits long-wavelength monochromatic light, which is mixed with short-wavelength monochromatic light emitted by the first synchrotron radiation light source 401 and then acted on the surface ion exciton resonant cavity of the microfluidic device for quantitative detection.

[0097] The second synchrotron radiation source 403 emits short-wave monochromatic light, which is mixed with the short-wave monochromatic light emitted by the first synchrotron radiation source 401 through the mirror 405 and then applied to the microfluidic chip of the microfluidic device 20 for continuous flow scanning to qualitatively detect structures such as α-helices and β-parallels with short wavelengths.

[0098] Fifthly, a method for multi-wavelength continuous scanning circular dichroism spectroscopy is provided, including the following steps:

[0099] S100: A shortwave band interval is established. Synchrotron radiation source 101 generates a composite monochromatic shortwave in the predetermined band interval. The shortwave passes through polarizer 102 and photoelectric modulator 103 and enters the microfluidic chip of the microfluidic device. The focusing area C spot is focused. The microfluidic device 20 mixes the liquid. The polarizer 102 and photoelectric modulator 103 are rotated to obtain the intensity of the corresponding polarization component of the output beam on the multi-wavelength monochromatic shortwave.

[0100] Based on the continuous scanning results, confirm whether to turn on the second synchrotron radiation source 403. Continuously generate composite monochromatic short waves in a predetermined band range according to the short wavelength band range, and continuously capture until the characteristic wavelength shows positive and negative Cotton peaks.

[0101] S200: Based on the continuous scanning results, determine whether to adjust the tilt angle of the microfluidic device 20, and then adjust the incident scanning angle between the microfluidic chip and the multi-wavelength monochromatic short wave, continuously capturing until the characteristic wavelength shows positive and negative peaks of Cotton.

[0102] S300: Establish a long-wavelength band range, analyze the characteristic peak distribution range of the sample to be tested, determine whether the multi-wavelength monochromatic light source 302 is combined with the synchrotron radiation light source 101 for multi-wavelength monochromatic short-wave scanning analysis of the characteristic peaks of the sample to be tested, the multi-wavelength monochromatic light source 302 generates long waves in the predetermined band range, which enter the surface ion exciton resonant cavity of the microfluidic device through the polarizer 102, and the tilt angle of the microfluidic device 20 is adjusted according to the continuous scanning results to output the CD intensity signal.

[0103] Through the above specific embodiments, those skilled in the art can easily implement the present invention. However, it should be understood that the present invention is not limited to the specific embodiments described above. Based on the disclosed embodiments, those skilled in the art can arbitrarily combine different technical features to achieve different technical solutions.

Claims

1. A multi-wavelength continuous scanning circular dichroism spectroscopy testing system, characterized in that: It includes several synchrotron radiation sources, polarizers, optoelectronic modulators, microfluidic devices, and optical paths; The synchrotron radiation source is fixed in a fixed hollow cavity, and there are several mirrors on one side of the source. The mirrors are used to separate the emitted waves of the synchrotron radiation source in the optical path channel. The synchrotron radiation source is connected to the polarizer, photoelectric modulator and microfluidic device, and is used to rotate the polarizer, photoelectric modulator and microfluidic device. The emitted wave is sent to the polarizer, photoelectric modulator and microfluidic device in sequence through the optical path channel. The synchrotron radiation source is also connected to the polarizer and the microfluidic device, which are used to rotate the polarizer and the microfluidic device, and the emitted wave is sent to the polarizer and the microfluidic device in sequence through the optical path channel; The multi-wavelength continuous scanning circular dichroism spectroscopy testing system also includes several detectors, which are used to detect the emitted waves passing through the microfluidic device.

2. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 1, characterized in that: The microfluidic device includes a microfluidic chip and a surface ion polariton resonant cavity, and the microfluidic chip and the surface ion polariton resonant cavity are connected through a liquid inlet pipe.

3. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 2, characterized in that: The emitted wave from the synchrotron radiation source is sequentially sent to the polarizer, photoelectric modulator, and microfluidic chip through the optical path channel, and the detector detects the emitted wave passing through the microfluidic chip.

4. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 2, characterized in that: The emitted wave from the synchrotron radiation source is sequentially sent to the polarizer and the surface ion resonant cavity through the optical path channel, and the detector detects the emitted wave passing through the surface ion resonant cavity.

5. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 2, characterized in that: The microfluidic device further includes a lower component and an upper component. The lower component and the upper component are respectively provided with a serpentine microfluidic channel region and a surface ion polariton resonant cavity region. The microfluidic chip and the surface ion polariton resonant cavity are respectively fabricated in the serpentine microfluidic channel region and the surface ion polariton resonant cavity region by pressing and fixing the lower component and the upper component. The consolidation bumps in the consolidation areas of the lower and upper components are bonded to the first dielectric layer in the consolidation groove, and the lower and upper components are tightly pressed together and sealed and bonded in the first dielectric layer.

6. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 2, characterized in that: The diameter of the liquid inlet channel of the surface ion polariton resonance cavity is more than 5 times that of the liquid inlet channel of the microfluidic chip.

7. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 2, characterized in that: The microfluidic chip includes at least two upper component bumps arranged at adjacent intervals and a lower component bump embedded in the upper component groove, forming a three-sided serpentine channel; The three-sided serpentine channel is connected to a synchrotron radiation scanning chamber.

8. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 2, characterized in that: The surface ion exciton resonant cavity includes an upper resonant cavity and a lower resonant cavity, wherein the upper resonant cavity is provided with a metal bump of the upper resonant cavity component and a groove of the upper resonant cavity component, and wherein the lower resonant cavity is provided with a metal bump of the lower resonant cavity component and a groove of the lower resonant cavity component. The upper component metal bumps of the resonant cavity are evenly spaced and distributed in the upper resonant cavity, forming grooves in the spaced areas; the lower component metal bumps of the resonant cavity are evenly spaced and distributed in the lower resonant cavity, forming grooves in the spaced areas. The metal bumps of the upper component of the resonant cavity correspond to the grooves of the lower component of the resonant cavity in the vertical direction, and do not abut against each other; the metal bumps of the lower component of the resonant cavity correspond to the grooves of the upper component of the resonant cavity in the vertical direction, and do not abut against each other.

9. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 2, characterized in that: In the surface ion polariton resonant cavity, the upper component metal bump and the lower component metal bump of the resonant cavity are simultaneously etched with the upper component bump and the lower component bump of the microfluidic chip by at least one of the processes of positive photolithography or negative photolithography.

10. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 2, characterized in that: The microfluidic device is encapsulated by a second dielectric layer. An optical waveguide region is provided on the sidewall of the surface ionotropic resonant cavity region. The surface ionotropic resonant cavity is connected to the first and second optical waveguides of the optical waveguide region, exposing the surface ionotropic resonant cavity and forming an incident window for the linearly polarized light emitted from the polarizer.

11. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 1, characterized in that: It also includes a multi-wavelength monochromatic light source, which is a long-wavelength monochromatic light source. An optical path intersection area is set. Through the mirror, the multi-wavelength monochromatic light source and the synchrotron radiation source mix the multi-wavelength monochromatic light and send it to the microfluidic device.

12. The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in claim 1, characterized in that: It also includes a multi-wavelength monochromatic light source, a first synchrotron radiation light source, and a second synchrotron radiation light source. The multi-wavelength monochromatic light source emits long-wavelength monochromatic light, which is mixed with short-wavelength monochromatic light emitted by the first synchrotron radiation light source through a mirror and then applied to the microfluidic device. The second synchrotron radiation source emits short-wave monochromatic light, which, after being mixed with the short-wave monochromatic light emitted by the first synchrotron radiation source through the mirror, acts on the microfluidic device.

13. A method for testing circular dichroism using multi-wavelength continuous scanning, characterized in that: The multi-wavelength continuous scanning circular dichroism spectroscopy testing system as described in any one of claims 1-12 is provided, comprising the following steps: S100: A shortwave band interval is established. A synchrotron radiation source generates multi-wavelength monochromatic shortwaves in a predetermined band interval. These shortwaves pass through a polarizer and a photoelectric modulator and enter the microfluidic chip of the microfluidic device. The light spot in the focusing area is detected and focused. The microfluidic device mixes liquids. The polarizer and the photoelectric modulator are rotated to obtain the intensity of the corresponding polarization component of the output beam on the multi-wavelength monochromatic shortwave. Based on the continuous scanning results, determine whether to turn on another synchrotron radiation source. Continuously generate multi-wavelength monochromatic short waves in a predetermined band range according to the short wavelength band range. The synchrotron radiation source and another synchrotron radiation source alternately output, continuously capturing until the characteristic wavelength shows Cotton positive and negative peaks. S200: Based on the continuous scanning results, determine whether to adjust the tilt angle of the microfluidic device, and then adjust the incident scanning angle between the microfluidic device and the multi-wavelength monochromatic short wave, continuously capturing until the characteristic wavelength shows positive and negative Cotton peaks; S300: Establish a long-wavelength band range, analyze the characteristic peak distribution range of the sample to be tested, determine whether the multi-wavelength monochromatic light source is combined with the multi-wavelength monochromatic short-wave scanning analysis of the characteristic peaks of the sample to be tested by the synchrotron radiation light source, the multi-wavelength monochromatic light source generates long waves in a predetermined band range, which enter the surface ion exciton resonant cavity of the microfluidic device through the polarizer, and adjust the tilt angle of the microfluidic device through the synchrotron radiation light source according to the continuous scanning results, and output CD signal.

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