Plant composition with emotion healing function and anti-oxidation soothing capability as well as preparation method and application of plant composition

Abstract

The invention belongs to the technical field of application of natural plant extracts in the fields of cosmetics and health-care products, and particularly relates to a plant composition with an emotion healing function and anti-oxidation soothing capacity as well as a preparation method and application of the plant composition. The traditional Chinese medicine composition is characterized by comprising the following components in percentage by weight: 0.003-0.07% of Chinese torreya seed extract, 0.003-0.07% of Chinese torreya seed extract, 0.003-0.07% of Chinese torreya seed extract and the balance 0.003% to 0.09% of a flower extract of crocus sativus (CROCUS SATIVUS); 0.003% to 0.09% of a centella asiatica (CENTTELLA ASIATICA) extract, and 0.003% to 0.09% of an extract of 0.003% to 0.08% of an oat (AVENA SATIVA) extract; 0.01% to 0.8% of 1, 2-hexanediol; 0.01% to 0.8% of p-hydroxyacetophenone; and the balance of deionized water.

Description

Technical Field

[0001] This invention belongs to the field of application technology of natural plant extracts in cosmetics and health products. More specifically, it relates to a plant composition with mood-healing function and antioxidant-soothing ability, as well as its preparation method and application. Background Technology

[0002] With the accelerating pace of modern life and increasing environmental pollution, people face ever-increasing pressure. Prolonged stress and anxiety lead to a growing number of skin problems, such as oxidative stress and sensitive skin. The skin is the largest sensory organ in the human body; its sensations influence emotional changes, which in turn affect the skin. Modern life and work pressures are constantly rising, often resulting in a sub-healthy state of mental tension, insufficient sleep, and negative emotions, leading to endocrine disorders, hormonal imbalances, and a series of problems that manifest on the skin as acne, redness, eye bags, dark circles, and accelerated aging. The skin and brain both originate from the ectoderm and share many hormones, neurotransmitters, and receptors, exhibiting a close connection and rich bidirectional communication mechanisms. Emotional healing typically refers to a skincare philosophy that focuses on the relationship between emotional health and skin health. Skincare products, as important tools for improving skin condition, can alleviate skin and emotional problems from different dimensions.

[0003] In recent years, people have become increasingly knowledgeable about the ingredients and effects of cosmetics, and their awareness of pursuing natural, green, safe, and healthy cosmetics has also grown stronger. Natural plant-based cosmetics, with their emphasis on nature, gentleness, non-irritation, and high safety, are deeply loved by consumers, and their development has become an inevitable trend in the cosmetics industry. Compared with chemically synthesized molecules, natural plants and traditional Chinese medicines exhibit multi-level and multi-target mechanisms of action. Plant raw materials mainly include flavonoids, alkaloids, and volatile oils, as well as polysaccharides, terpenes, and saponins, which respectively possess antioxidant, anti-inflammatory, soothing, and repairing effects.

[0004] The application of antioxidants and anti-sensitivity agents in cosmetics and pharmaceuticals is receiving increasing attention. Natural plants, due to their high safety and minimal side effects, have become a research hotspot. However, current market products with antioxidant and skin-soothing effects often have limited efficacy and single-ingredient formulations, failing to meet consumer needs. Current development of antioxidant and mood-healing cosmetics mostly utilizes chemically synthesized molecules or compounds of chemically synthesized molecules with certain plant extracts, addressing only a single pathway and failing to fundamentally solve skin problems. Therefore, this study uses a combination of multiple natural plant extracts to coordinate multiple pathways, preparing a composition with antioxidant, anti-inflammatory, and skin-soothing effects to improve sensitive skin problems. Summary of the Invention

[0005] The present invention addresses the above-mentioned problems by providing a plant composition with emotional healing function and antioxidant soothing ability, as well as its preparation method and application.

[0006] To achieve the above objectives, the present invention adopts the following technical solution, the composition and weight percentage of which are: Torreya grandis seed extract 0.003%–0.07%; Crocus sativus flower extract 0.003%–0.09%; Centella asiatica extract 0.003%–0.09%; Avena sativa extract 0.003%–0.08%; 1,2-hexanediol 0.01%–0.8%; p-hydroxyacetophenone 0.01%–0.8%; the remainder being deionized water.

[0007] The preferred composition and weight percentages are as follows: Torreya grandis seed extract 0.003%–0.06%; saffron flower extract 0.003%–0.08%; Centella asiatica extract 0.003%–0.08%; oat extract 0.003%–0.07%; 1,2-hexanediol 0.01%–0.8%; p-hydroxyacetophenone 0.01%–0.8%.

[0008] The preferred composition and weight percentages are as follows: Torreya grandis seed extract 0.053%; Crocus sativus flower extract 0.078%; Centella asiatica extract 0.076%; Avena sativa extract 0.064%; 1,2-hexanediol 0.5%; p-hydroxyacetophenone 0.5%; the remainder being deionized water. This formulation can further improve the hyaluronidase inhibition rate, ABTS free radical scavenging rate, and DPPH free radical scavenging rate.

[0009] The specific preparation method is as follows: (1) Disperse the Torreya grandis seed powder in deionized water at a ratio of 1g / 10mL, stir and extract at 40-90℃ for 0.5-10h, centrifuge the resulting extract at 500-9000rpm for 5-60min, filter, and the filtrate is Torreya grandis seed extract. (2) Mix dried saffron petal powder with deionized water at a ratio of 1 g / 10 mL for 5-40 min. Centrifuge the extract at 500-9000 rpm for 5-60 min, collect the supernatant, filter, and the filtrate is saffron flower extract. (3) Mix dried Centella Asiatica powder with deionized water at a ratio of 1 g / 10 mL for 5-40 min. Centrifuge the extract at 500-8000 rpm for 5-60 min, collect the supernatant, filter, and the filtrate is Centella Asiatica extract. (4) Weigh the dried oat powder according to the ratio of 1 g / 10 mL, put it into a beaker, add deionized water, and mix it evenly with a magnetic stirrer. Centrifuge the resulting extract at 500-8000 rpm for 5-60 min, collect the supernatant, filter it, and the filtrate is the oat (AVENA SATIVA) extract. (5) Mix 1,2-hexanediol, p-hydroxyacetophenone, Torreya grandis seed extract, Crocus sativus flower extract, Centella asiatica extract, Avenasatia var. oat extract and deionized water according to the above ratio, and stir until completely dissolved without particles to obtain the final product.

[0010] The plant-based compositions of the present invention can be used as the main components of antioxidant and soothing cosmetics.

[0011] The beneficial effects of this invention are: Torreya grandis, one of my country's ten major woody oilseed tree species, belongs to the genus Torreya in the family Taxaceae. Its seeds are mainly distributed in Zhejiang, southern Anhui, northern Fujian, and northeastern Jiangxi, with a long history of cultivation in Zhuji and Dongyang, Zhejiang. Torreya grandis seeds are rich in various bioactive components, including phenolic compounds, fatty acids, β-sitosterol, and γ-tocopherol. These components endow the seeds with significant antioxidant activity, inhibiting free radicals and lipid peroxidation, thus possessing anti-aging potential. Furthermore, the seeds contain high levels of fatty oils and soluble proteins, giving them broad application prospects in the food and pharmaceutical fields. The potassium content of Torreya grandis seed kernels is as high as 7.0–11.8 g·kg⁻¹, the highest among all dried fruits. Modern medical research has proven that potassium plays a significant role in maintaining heart function, participating in metabolism, and lowering blood pressure; it also helps regulate emotions and stabilize mood.

[0012] Saffron (Crocus sativus L.), also known as crocus, contains crocin, crocinin, and crocin glycosides as its main active components, which endow it with a variety of biological activities. Studies have shown that saffron has significant antioxidant, anti-inflammatory, anti-tumor, and antidepressant effects. For example, crocin can scavenge free radicals and inhibit lipid peroxidation; crocin has a protective effect on the liver and can reduce alcoholic liver damage. In addition, saffron has shown potential application value in the treatment of cardiovascular and cerebrovascular diseases, gynecological diseases, and anti-tumor effects. In recent years, with in-depth research, the pharmacological mechanism of saffron has been gradually revealed, and its application prospects in medicine, food, and cosmetics are broad. Among them, saffron has a unique function in emotional healing: crocin can inhibit apoptosis and inflammatory response of cortical neurons damaged by hypoxia-reoxygenation, and has a protective effect on cortical neurons damaged by hypoxia-reoxygenation. Its mechanism may be related to the inhibition of the TLR4 / NF-κB signaling pathway. Furthermore, experiments have shown that crocin improves depressive-like behavior in depressed rats by activating the cAMP / PKA / CREB signaling pathway, reducing inflammation, and increasing hippocampal neurons. Other studies have shown that crocin and crocin extracted from saffron can effectively increase blood levels of GABA and serotonin, thereby regulating mood and improving sleep. Additionally, anthocyanins and other components in saffron can increase levels of neurotransmitters such as endorphins and serotonin in the brain, alleviating anxiety and depression.

[0013] Oats (Avena sativa L.) are a globally cultivated crop, primarily distributed in temperate regions of the Northern Hemisphere. Oats are classified into hulled (hulled) and naked (naked) varieties. China mainly cultivates naked oats, primarily for food. Oats are rich in various bioactive components, including beta-glucan, protein, phenolic compounds, vitamin E, and unsaturated fatty acids. Beta-glucan is the main functional component of oats, possessing bioactivity that lowers blood lipids, lowers blood sugar, and regulates immune function. Oat protein content is as high as 16%, with a balanced amino acid composition, especially rich in lysine, making its nutritional value higher than that of ordinary grains. Furthermore, phenolic compounds in oats (such as ferulic acid and caffeic acid) and avena anthracene amides have significant antioxidant and anti-inflammatory effects, helping to prevent chronic diseases. In recent years, oats have shown broad application prospects in functional foods and medicine, particularly in research on lowering lipids, lowering blood sugar, and anti-aging. Oat extract is rich in tryptophan, which helps the brain secrete melatonin (a physiological hormone that regulates sleep and circadian rhythms), making it easier to fall asleep.

[0014] Centella asiatica (L.) Urban, a commonly used traditional Chinese medicine and food, has the effects of clearing heat and dampness, detoxifying and reducing swelling. It is rich in chemical components, mainly including triterpenoids, polyacetylenes, volatile oils, and flavonoids, possessing anti-inflammatory, antioxidant, anti-tumor, and antidepressant pharmacological effects. Its extracts have a significant promoting effect on wound and ulcer healing. Studies have shown that asiaticoside can inhibit the activity of monoamine oxidase-A (MAO-A) in the hippocampus, cortex, and hypothalamus, while also inhibiting the activity of monoamine oxidase-B (MAO-B) in the cortex and hypothalamus, reducing MAO activity in the brain and producing antidepressant activity. Literature reports that total asiaticosides can increase the levels of aspartic acid (Asp), glycine (Gly), and taurine (Tau), and decrease the level of alanine (Ala), thus exhibiting an antidepressant effect.

[0015] This invention scientifically studies the proportions of Torreya grandis seed extract, Crocus sativus flower extract, Centella asiatica extract, and Avena sativa extract to obtain a composition with excellent antioxidant and skin-soothing effects.

[0016] This invention combines various natural plant and traditional Chinese medicine extracts to create a composition with antioxidant and skin-soothing effects through multi-pathway coordination, thereby improving sensitive skin problems.

[0017] Experimental results show that the plant composition prepared in this invention has an inhibition rate of 42.45-100% against hyaluronidase, an ABTS free radical scavenging rate of 72.57-90.83%, a hydroxyl free radical scavenging rate of 56.78-69.61%, and a DPPH free radical scavenging rate of 52.52-79.22%. Attached Figure Description

[0018] Figure 1 The antioxidant test results of the single plant extracts (Torreya grandis seed extract: AC; Crocus sativus flower extract: DF; Centella asiatica extract: GI; Avena sativa extract: JK) obtained in Examples 2-5 of this invention are shown. Figure 2The results of the experiments on the inhibitory activity of single plant extracts (A- Torreya grandis seed extract; B- Crocus sativus flower extract; C- Centella asiatica extract; D- Avena sativa extract) obtained in Examples 6-9 of the present invention on the inhibition of hyaluronidase. Figure 3 The results of the antioxidant (AC) and hyaluronidase inhibitory activity (D) experiments of the compositions obtained in Examples 10-11 of the present invention are shown. Figure 4 The results are experimental results of the hyaluronidase inhibitory activity of the compositions obtained in Example 1 and Comparative Examples 1-17 of this invention. Detailed Implementation

[0019] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention. These all fall within the scope of protection of the present invention.

[0020] Although the numerical ranges and parameters illustrating the broad scope of the invention are approximate, the values ​​listed in the specific examples are reported as precisely as possible. However, any numerical value inherently contains some error that is necessarily caused by the standard deviation found in their respective test measurements.

[0021] In the following examples, all raw materials and reagents used are commercially available products or prepared by conventional methods, and the present invention does not impose any particular limitations.

[0022] Example 1 Take 1.06g of Torreya grandis seed extract, 1.56g of Crocus sativus flower extract, 1.52g of Centella asiatica extract, and 1.3g of Avena sativa extract, place them in 1974.58g of deionized water, add 10g of 1,2-hexanediol and 10g of p-hydroxyacetophenone, and stir at room temperature until there are no particles. The resulting mixture is Example 1.

[0023] Example 2 This embodiment evaluated the antioxidant activity of Torreya grandis seed extract against ABTS, hydroxyl, and DPPH free radicals at different concentration gradients, as shown in the figure. Figure 1 As shown in (AC). Its preparation method is the same as in Example 1.

[0024] Example 3 This embodiment evaluated the antioxidant activity of saffron (CROCUS SATIVUS) flower extract against ABTS, hydroxyl, and DPPH free radicals at different concentration gradients, as shown in the figure. Figure 1 As shown in (DF). Its preparation method is the same as that in Example 1.

[0025] Example 4 This embodiment evaluated the antioxidant activity of Centella Asiatica extract against ABTS, hydroxyl, and DPPH free radicals at different concentration gradients, as shown in the figure. Figure 1 As shown in (GI). Its preparation method is the same as in Example 1.

[0026] Example 5 This embodiment evaluated the antioxidant activity of oat (AVENA SATIVA) extract against ABTS, hydroxyl, and DPPH free radicals at different concentration gradients, as shown in the figure. Figure 1 As shown in (JL). Its preparation method is the same as that in Example 1.

[0027] Example 6 This embodiment evaluated the inhibitory effect of Torreya grandis seed extract on hyaluronidase activity at different concentration gradients, such as... Figure 2 As shown in (A). Its preparation method is the same as that in Example 1.

[0028] Example 7 This embodiment evaluated the inhibitory activity of hyaluronidase by crocus sativis flower extract at different concentration gradients, such as... Figure 2 As shown in (B), its preparation method is the same as in Example 1.

[0029] Example 8 This embodiment evaluated the inhibitory activity of Centella Asiatica extract on hyaluronidase at different concentration gradients, such as... Figure 2 As shown in (C), its preparation method is the same as that in Example 1.

[0030] Example 9 This embodiment evaluated the inhibitory activity of oat (AVENA SATIVA) extract on hyaluronidase at different concentration gradients, such as... Figure 2 As shown in (D). Its preparation method is the same as that in Example 1.

[0031] Example 10 This embodiment evaluated the antioxidant activity of various plant extracts after being diluted tenfold with purified water at different compound volume ratios against ABTS, hydroxyl, and DPPH free radicals. Figure 3 As shown in (A). Its preparation method is the same as that in Example 1.

[0032] Example 11 This embodiment evaluated the hyaluronidase inhibitory activity of various plant extracts after being diluted tenfold with purified water at different compound volume ratios, such as... Figure 3 As shown in (D). Its preparation method is the same as that in Example 1.

[0033] Comparative Example 1 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities. The composition and volume fractions are shown in Table 1. Its preparation method is the same as in Example 1.

[0034] Comparative Example 2 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0035] Comparative Example 3 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0036] Comparative Example 4 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0037] Comparative Example 5 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0038] Comparative Example 6 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0039] Comparative Example 7 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0040] Comparative Example 8 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0041] Comparative Example 9 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0042] Comparative Example 10 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0043] Comparative Example 11 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0044] Comparative Example 12 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0045] Comparative Example 13 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0046] Comparative Example 14 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0047] Comparative Example 15 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0048] Comparative Example 16 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0049] Comparative Example 17 This comparative example provides a plant composition with emotional healing and antioxidant soothing capabilities, and its preparation method. The components and volume parts of the plant composition are shown in Table 1. The preparation method is the same as in Example 1.

[0050] The formulations of Example 1 and Comparative Examples 1-17 are shown in Table 1 below.

[0051] Table 1 Torreya grandis seed extract (g) Saffron (CROCUSSATIVUS) flower extract (g) Centella Asiatica Extract (g) Oat (AVENASATIVA) extract (g) Deionized water (g) 1,2-Hexanediol (g) p-Hydroxyacetophenone (g) Example 1 1.06 1.56 1.52 1.3 1974.58 50 50 Comparative Example 1 1.06 0.71 1.50 1.3 1974.58 50 50 Comparative Example 2 1.06 1.56 1.52 1.3 2100.41 50 50 Comparative Example 3 1.06 1.05 1.52 1.3 1974.58 50 50 Comparative Example 4 1.06 1.56 1.02 1.3 1974.58 50 50 Comparative Example 5 1.06 1.56 1.52 1.0 1974.58 50 50 Comparative Example 6 1.06 1.56 1.02 1.3 2100.41 50 50 Comparative Example 7 1.02 1.06 1.52 1.3 1974.58 50 50 Comparative Example 8 0.56 1.11 1.42 1.15 1975 50 50 Comparative Example 9 0.65 0.65 1.42 1.3 1975 50 50 Comparative Example 10 0.65 1.11 1.5 1.3 1975 50 50 Comparative Example 11 1.00 0.87 1.45 1.00 1975 50 50 Comparative Example 12 1.05 0.98 1.42 0.95 1975 50 50 Comparative Example 13 0.98 1.05 1.00 1.05 1975 50 50 Comparative Example 14 0.95 1.10 1.05 1.10 1975 50 50 Comparative Example 15 0.95 0.65 1.11 1.10 1975 50 50 Comparative Example 16 0.95 1.15 1.24 1.24 1975 50 50 Comparative Example 17 1.06 1.20 1.52 1.3 1975 50 50 Study on the antioxidant effect of plant composition Experimental Example 1: Determination of ABTS Free Radical Scavenging Rate The plant compositions with mood-healing and antioxidant-soothing abilities prepared in each embodiment and comparative example were tested according to the ABTS free radical scavenging rate test method described below.

[0052] 7 mmol / L ABTS + Mix the solution with an equal volume of 2.45 mmol / L potassium persulfate solution and let it stand in the dark at room temperature for 12–16 h. Before use, dilute it with anhydrous ethanol to achieve an absorbance of 0.7 ± 0.02 at 734 nm. + Working solution. After the mixture was incubated in the dark for 6 min, its absorbance was measured at 734 nm using a full-wavelength microplate reader. ABTS was calculated using the following formula. + Free radical scavenging rate: ABTS + Free radical scavenging rate (%) =

[0053] In the formula: A0 is 600 μL of anhydrous ethanol plus 2.4 mL of ABTS + Absorbance of the working solution; A1 is 600 μL of enzyme digest plus 2.4 mL of ABTS + The absorbance of the working solution; A2 is the absorbance of 600 μL of enzyme hydrolysate with 2.4 mL of anhydrous ethanol.

[0054] Experimental Example 2: Determination of Hydroxyl Radical Scavenging Rate The single plant extracts and plant compositions with antioxidant and soothing effects prepared in each example and comparative example were tested according to the hydroxyl radical scavenging test method described below.

[0055] The salicylic acid-ferrous sulfate method was used. 1 mL each of 9 mmol / L salicylic acid ethanol solution, 9 mmol / L FeSO4, sample solutions of different concentrations, and 8.8 mmol / L H2O2 were added sequentially to the reaction system. After reacting in a constant temperature water bath at 37℃ for 15 min, the absorbance of the reaction solution was measured at 510 nm. The hydroxyl radical scavenging rate was calculated using the following formula: Hydroxyl radical scavenging rate (%) =

[0056] In the formula: A0 is the absorbance of the reaction system in which deionized water is used to replace the sample; A1 is the absorbance of the reaction system in which the sample is added; A2 is the absorbance of the reaction system in which deionized water is used to replace the H2O2 solution.

[0057] Experimental Example 3: Determination of DPPH Group Scavenging Rate Accurately weigh 0.004 g DPPH and dilute to 100 mL with anhydrous ethanol. Let stand for no more than 4 hours to obtain the DPPH solution. Incubate the mixture in a 96-well plate in the dark for 30 min, then measure the absorbance at 517 nm using a full-wavelength microplate reader. Calculate the DPPH free radical scavenging rate using the following formula: DPPH free radical scavenging rate (%) =

[0058] In the formula: A0 is the absorbance of 50 μL of anhydrous ethanol plus 100 μL of DPPH working solution; A1 is the absorbance of 50 μL of enzyme digest plus 100 μL of DPPH working solution; A2 is the absorbance of 50 μL of enzyme digest plus 100 μL of anhydrous ethanol.

[0059] Antioxidant test results as follows Figure 1 and Figure 3 As shown in (AC), the single plant extracts of Examples 2-5 ( Figure 1 ) and the plant composition of Example 10 Figure 3Both (AC) exhibited excellent ABTS, hydroxyl, and DPPH radical scavenging abilities, demonstrating strong antioxidant capacity. The scavenging rate of single plant extracts gradually increased with increasing concentration, while the combination of plant compositions maintained a relatively high scavenging rate. In Example 10, the optimal plant composition ratios for ABTS, hydroxyl, and DPPH radical scavenging experiments (Torreya grandis seed extract: Crocus sativus flower extract: Centella asiatica extract: Avena sativa extract) were 3:2:5:4 (90.83%), 3:2:5:4 (69.61%), and 3:5:3:2 (79.22%), respectively.

[0060] Study on the soothing effects of plant-based compositions on skin mood Experiment Example 4: Hyaluronidase Inhibition Experiment The single plant extracts and plant compositions with antioxidant and skin-soothing effects prepared in each embodiment and comparative example were tested according to the hyaluronidase inhibition test method described below. The test results are as follows: Figure 2 , Figure 3 (D) and Figure 4 As shown.

[0061] Prepare the following solutions separately: pH 5.6 acetate buffer (0.2 mol / L acetic acid solution and 0.2 mol / L sodium acetate solution mixed in a certain ratio), 2.5 mmol / L calcium chloride solution, 600 u / mL hyaluronidase solution, 0.5 mg / mL sodium hyaluronate solution, 0.4 mol / L sodium hydroxide solution, acetylacetone solution, and Ehrlich reagent (0.8 g p-dimethylaminobenzaldehyde dissolved in 15 mL concentrated hydrochloric acid and 15 mL anhydrous ethanol).

[0062] Add reagents according to Table 2, mix the system and develop color at room temperature for 20 min. After the process, measure the absorbance at 530 nm using an ELISA reader and calculate the hyaluronidase activity inhibition capacity of the plant composition. Each group has three parallel experiments.

[0063] Table 2 Hyaluronidase Activity Inhibition Experiment The inhibition rate of the plant composition on hyaluronidase activity was calculated using the following formula: Hyaluronidase inhibition rate (%) = ; In the formula: C is the absorbance of the sample group, D is the absorbance of the sample control group, A is the absorbance of the negative control group, and B is the absorbance of the blank control group.

[0064] The test results for inhibiting hyaluronidase activity are as follows: Figure 2 , Figure 3 (D) and Figure 4 As shown. Single plant extracts from Examples 6-9 ( Figure 2 ) and Example 11 ( Figure 3 (D) and comparative example ( Figure 4 The plant compositions all have excellent hyaluronidase inhibitory activity, which can inhibit the degradation of hyaluronic acid and reduce the release of inflammatory factors to achieve a soothing effect. Figure 3 (D) The plant composition exhibited the highest hyaluronidase inhibitory activity of 99.82%, with a volume ratio of 3:2:5:4 for the composition (Torreya grandis seed extract: Crocus sativus flower extract: Centella asiatica extract: Avena sativa extract). Based on this, to further explore the optimal ratio of plant compositions for hyaluronidase inhibition, comparative experiments of hyaluronidase inhibition were conducted in proportions 1-17. Figure 4 The experimental results showed that all of them exhibited significant hyaluronidase inhibitory activity. The optimal compound ratio of the plant composition (torreya grandis seed extract, crocus sativus flower extract, centella asiatica extract, and avena sativa extract) was 3:5:5:4, and the hyaluronidase inhibition rate could reach up to 100%.

[0065] Experiment Example 5: Sensory evaluation of mood change state within 30 minutes after using the optimally proportioned plant composition.

[0066] This experiment invited 15 randomly selected passersby as subjects, covering different age groups, genders, and occupations. Among them, 8 were male and 7 were female; their occupations included students, office workers, and freelancers. The experiment was conducted in a quiet and comfortable indoor laboratory to minimize external environmental interference with the participants' mood. The experiment recorded the participants' emotional states before and after using the plant composition with skin-soothing effects described in this patent.

[0067] Table 3 serial number Emotional state before use Emotional state within 30 minutes after use 1 calm Pleasure 2 calm Pleasure 3 calm Pleasure 4 calm calm 5 calm Pleasure 6 calm calm 7 Unpleasant calm 8 calm Pleasure 9 calm calm 10 Pleasure Pleasure 11 calm Pleasure 12 calm calm 13 Unpleasant Pleasure 14 calm calm 15 Pleasure Pleasure Based on the above data, after using the plant composition with skin-soothing and mood-enhancing effects described in this patent, users numbered 1, 2, 3, 10, 8, and 11 experienced a shift from calm to pleasant emotions, indicating that the plant composition has a positive effect on improving mood. Users numbered 7 and 13 experienced a shift from unpleasant to calm or pleasant emotions, indicating that the plant composition also has a positive effect on alleviating negative emotions.

[0068] This plant-based composition is suitable for most commercially available cosmetic formulations, such as water, spray, lotion, oil-in-water cream, and water-in-oil cream. The temperature should be below 45℃ when adding the material, and it should be stirred until homogeneous. The recommended addition amount is 0.01–2%. The odor and color of this plant-based composition will not significantly affect the original odor and color of the product. After three months of testing under high and low temperature and light conditions in the laboratory, the odor and skin feel of the product remained similar to the initial state; the viscosity, pH, relative density, and other physicochemical values ​​were all within the normal range.

[0069] Overall, most testers experienced an improvement in their emotional state after using the product, particularly a significant shift from calm to pleasure, which may indicate that the plant composition has a soothing and mood-enhancing effect. Some testers maintained stable emotions after using the product, suggesting that the plant composition helps maintain emotional balance, a positive effect for those who need emotional stability. The product also showed mood-improving effects for testers who initially experienced unpleasant emotions, potentially indicating a positive role for managing stress and negative emotions. Based on the test data, the soothing plant composition of this invention has a positive impact on improving and stabilizing mood. It not only enhances mood but also helps alleviate negative emotions, making users feel more pleasant and relaxed. These improvements may contribute to improved quality of life, reduced stress, and enhanced overall well-being.

[0070] This invention has many specific applications, and the above description is only a preferred embodiment. It should be noted that the above embodiments are for illustrative purposes only and are not intended to limit the scope of protection of this invention. For those skilled in the art, several improvements can be made without departing from the principle of this invention, and these improvements should also be considered within the scope of protection of this invention.

Claims

1. A plant composition having emotional healing function and antioxidant soothing ability, characterized in that, The composition and weight percentage are as follows: Torreya grandis seed extract 0.003%-0.07%; crocus sativus extract 0.003%-0.09%; Centella asiatica extract 0.003%-0.09%; oat extract 0.003%-0.08%; 1,2-hexanediol 0.01%-0.8%; p-hydroxyacetophenone 0.01%-0.8%; and the rest is deionized water.

2. The plant composition having emotional healing function and antioxidant soothing ability according to claim 1, characterized in that, The composition and weight percentage are as follows: Torreya grandis seed extract 0.003%-0.07%; crocus sativus extract 0.003%-0.09%; Centella asiatica extract 0.003%-0.09%; oat extract 0.003%-0.08%; 1,2-hexanediol 0.01%-0.8%; p-hydroxyacetophenone 0.01%-0.8%.

3. The method for preparing the plant composition with emotional healing function and antioxidant soothing ability as described in claim 1, characterized in that, The specific preparation method is as follows: (1) dispersing Torreya grandis seed powder in deionized water at a material-to-liquid ratio of 1 g / 10 mL, stirring and leaching at 40-90°C for 0.5-10 h, centrifuging the obtained extract at 500-9000 rpm for 5-60 min, filtering, and the filtrate is Torreya grandis seed extract; (2) mixing dry crocus sativus petal powder with deionized water at a material-to-liquid ratio of 1 g / 10 mL for 5-40 min, centrifuging the extract at 500-9000 rpm for 5-60 min, collecting the supernatant, filtering, and the filtrate is crocus sativus extract; (3) mixing dry Centella asiatica powder with deionized water at a material-to-liquid ratio of 1 g / 10 mL for 5-40 min, centrifuging the extract at 500-8000 rpm for 5-60 min, collecting the supernatant, filtering, and the filtrate is Centella asiatica extract; (4) weighing dry oat powder at a material-to-liquid ratio of 1 g / 10 mL, putting it into a beaker, adding deionized water, mixing uniformly with a magnetic stirrer during the process, centrifuging the obtained extract at 500-8000 rpm for 5-60 min, collecting the supernatant, filtering, and the filtrate is oat extract; (5) mixing 1,2-hexanediol, p-hydroxyacetophenone, Torreya grandis seed extract, crocus sativus extract, Centella asiatica extract, oat extract, and deionized water according to the above proportions, stirring to dissolve completely without granules, and the final product is obtained.

4. The use of the plant composition having emotional healing function and antioxidant soothing ability according to claim 1, characterized in that, It can be used as the main component of antioxidant soothing cosmetics.

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