Cryopreservation device and method for infectious disease cell culture

Abstract

The invention relates to the technical field of cell cryopreservation, in particular to a cryopreservation device for infectious disease cell culture, which comprises a cryopreservation refrigerator and a cryopreservation box arranged in the cryopreservation refrigerator, the cryopreservation box comprises a box body, a bearing ring, a cold gathering base and an air supply mechanism, the bearing ring is arranged in the box body and used for allowing the lower portion of a test tube containing an infectious disease cell sample to penetrate through and bearing the upper portion of the test tube, the cold gathering base is arranged in the box body and located under the bearing ring, and a cryopreservation cavity allowing the lower portion of the test tube to stretch into is formed in the upper portion of the cold gathering base. The box body is provided with a temperature sensor arranged in the cryopreservation cavity, the air supply mechanism is installed on the box body and used for blowing air in the cryopreservation refrigerator to the lower portion of the cold gathering base, and the cryopreservation refrigerator is provided with a controller used for being in communication connection with the temperature sensor and the air supply mechanism. The invention further discloses a cryopreservation method for infectious disease cell culture. By controlling the working state of the air supply mechanism, the cryopreservation effect of infectious disease cell culture can be ensured.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell cryopreservation, and particularly relates to a freezing device and method for infectious disease cell culture. BACKGROUND

[0002] Infectious disease cell culture plays a crucial role in medical research, disease diagnosis, vaccine development and treatment strategy formulation. Based on the requirements of long-term preservation of infectious disease cells, prevention of contamination, backup, sharing of transport boxes, continuity of research, reduction of variation, optimization of resources, biological safety and the like, the freezing of infectious disease cells is an important link in cell biology and virology research. Freezing cells is a process that requires precise operation to ensure that the cells can recover activity after thawing.

[0003] In a laboratory, in order to preserve cells for a long time, a step freezing box (also known as a freezing box or cell freezing box) is usually used to store freezing tubes. First, the cell suspension is mixed with an appropriate amount of cryoprotectant (such as glycerol, dimethyl sulfoxide or propylene glycol), and the mixed cell suspension is dispensed into freezing tubes; the freezing tubes containing the cell suspension are placed into the step freezing box; the step freezing box is placed into the freezing refrigerator and the freezing tubes containing the cell suspension are placed into the step freezing box; until the temperature drops to the required temperature, the step freezing box is transferred to an ultra-low temperature refrigerator for preservation.

[0004] In the prior art, when using a step freezing box, the step freezing generally has a cold ring, which is usually made of a noble metal alloy material such as gold, silver, copper, platinum and the like, which has excellent heat conduction capacity and high heat capacity. Generally, the cold ring will be placed at a specific position of the freezing box, and the freezing tubes will be placed around the cold ring to take advantage of its rapid heat conduction characteristics. Before placing the freezing tubes into the cold ring, it is ensured that the cold ring has been sufficiently pre-cooled to achieve the best freezing effect.

[0005] However, since only the temperature in the freezing refrigerator can be controlled to change according to the set temperature-time curve during freezing, the temperature of the freezing space in the freezing box for freezing the freezing tubes cannot be fed back and the freezing speed of the freezing space cannot be changed, resulting in a large difference between the temperature-time curve in the freezing space and the set temperature-time curve, which affects the freezing effect of the infectious disease cell culture. SUMMARY

[0006] To solve the above technical problems, the present application provides a freezing device and method for infectious disease cell culture.

[0007] In a first aspect, the present application provides a freezing device and method for infectious disease cell culture, comprising a freezing refrigerator and a freezing box used in the freezing refrigerator; the freezing box comprises a box body, a supporting ring, a cold seat and a blowing mechanism, the supporting ring is arranged in the box body and used for allowing the lower part of a test tube containing an infectious disease cell sample to pass through and supporting the upper part of the test tube, the cold seat is arranged in the box body and located directly below the supporting ring, the upper part of the cold seat is provided with a freezing cavity for the lower part of the test tube to extend into, the box body is provided with a temperature sensor arranged in the freezing cavity, and the blowing mechanism is installed on the box body and used for blowing gas in the freezing refrigerator to the lower part of the cold seat; and the freezing refrigerator is provided with a controller used for communication connection with the temperature sensor and the blowing mechanism.

[0008] The freezing device for infectious disease cell culture can control the cooling speed of the cold seat by controlling the working state of the blowing mechanism, so that the temperature-time curve in the freezing cavity is more close to the preset temperature-time curve, and the freezing effect of the infectious disease cell culture is ensured.

[0009] As an improvement of the first aspect, the lower part of the cold seat is provided with a wind cooling cavity, a wind cooling inlet in communication with the wind cooling cavity and a wind cooling outlet in communication with the wind cooling cavity, the box body is provided with an air inlet pipeline in communication with the outside of the box body and the wind cooling inlet and an air outlet pipeline in communication with the outside of the box body and the wind cooling outlet, and the blowing mechanism is used for driving the gas in the freezing refrigerator to sequentially pass through the air inlet pipeline, the wind cooling cavity and the air outlet pipeline, so as to ensure that the gas blown by the blowing mechanism contacts the lower part of the cold seat and the heat exchange effect is ensured, and the temperature of the cold seat is better controlled.

[0010] As an improvement of the first aspect, the wind cooling inlet is in communication with the lower left part of the wind cooling cavity, the wind cooling outlet is in communication with the lower right part of the wind cooling cavity, two partitions are arranged in the wind cooling cavity and are arranged in parallel and downwardly inclined from left to right, the connection between the lower end of the partition close to the wind cooling inlet and the wind cooling cavity and the connection between the upper end of the partition close to the wind cooling outlet and the wind cooling cavity are both provided with air vents, and the residence time of the gas in the wind cooling cavity is prolonged, so as to ensure the heat transfer effect of the gas and the cold seat.

[0011] As an improvement of the first aspect, the freezing box further comprises a freezing rack arranged in the box body, the freezing rack is provided with a plurality of the freezing rings, a plurality of the cold seats are arranged one by one below the freezing rings, and a plurality of the temperature sensors are arranged one by one in the freezing cavities, so that a plurality of test tubes can be simultaneously frozen, and the freezing efficiency of the infectious disease cells is improved.

[0012] As an improvement of the first aspect, the air inlet pipeline comprises an air inlet duct and a plurality of air inlet branch pipes in communication with the air inlet duct and each provided with an air inlet solenoid valve rod, the plurality of air inlet branch pipes are in communication with the air cooling inlets of the plurality of cold storage seats; the air outlet pipeline comprises an air outlet duct and a plurality of air outlet branch pipes in communication with the air outlet duct and each provided with an air outlet solenoid valve rod, the plurality of air outlet branch pipes are in communication with the air cooling inlets of the plurality of cold storage seats; the air inlet solenoid valve rod and the air outlet solenoid valve rod are electrically connected with the controller to control the cooling speed of the plurality of cold storage seats respectively, so as to control the temperature of the plurality of cold storage chambers respectively.

[0013] As an improvement of the first aspect, the air supply mechanism comprises a first pressing air bag and a first driving component for driving the first pressing air bag to expand or shrink, the first pressing air bag is provided with a first air inlet pipe arranged in the freezing refrigerator and a first air outlet pipe in communication with the air inlet duct, and the first driving component is connected with the controller to realize the air supply function of the air supply mechanism.

[0014] As an improvement of the first aspect, the air supply mechanism comprises a second pressing air bag and a second driving component for driving the second pressing air bag to expand or shrink, the second pressing air bag is provided with a second air inlet pipe arranged in the air outlet duct and a second air outlet pipe arranged in the freezing refrigerator, and the second driving component is connected with the controller, so that the air supply capacity range of the air supply mechanism can be adjusted, and a more suitable and controllable cooling speed is provided for the cold storage seat.

[0015] In the second aspect, the present application provides a freezing method for infectious disease cell culture, which adopts the freezing device for infectious disease cell culture in the first aspect, and the freezing method comprises the following steps:

[0016] S1, setting the freezing temperature time curve of the freezing refrigerator in the controller;

[0017] S2, placing the test tube containing the infectious disease cell sample into the freezing box, so that the lower part of the test tube penetrates through the supporting ring and extends into the freezing chamber;

[0018] S3, placing the freezing box containing the test tube into the freezing refrigerator, and connecting the temperature sensor and the air supply mechanism with the controller in communication;

[0019] S4, the controller controls the air supply mechanism to work according to the detection value of the temperature sensor.

[0020] As an improvement of the second aspect, in step S4,

[0021] if the actual temperature value of the temperature sensor read by the controller is less than the preset temperature value corresponding to the time on the freezing temperature time curve, the controller controls the air supply mechanism to stop working;

[0022] If the controller reads the actual temperature value of the temperature sensor is greater than or equal to the preset temperature value corresponding to the time on the freeze temperature-time curve, the controller controls the air supply mechanism to keep working.

[0023] As a second face technical solution improvement, in step S4:

[0024] If the controller reads the actual temperature value of the temperature sensor is less than the preset temperature value corresponding to the time on the freeze temperature-time curve, the controller controls the air supply mechanism to reduce the working frequency;

[0025] If the controller reads the actual temperature value of the temperature sensor is greater than or equal to the preset temperature value corresponding to the time on the freeze temperature-time curve, the controller controls the air supply mechanism to increase the working frequency. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 It is a structure schematic view of a freeze device for infectious disease cell culture in the embodiment of the present application;

[0027] Figure 2 It is a structure schematic view of a freeze box in the embodiment of the present application;

[0028] Figure 3 It is a working principle diagram of a cold collection seat in the embodiment of the present application;

[0029] Figure 4 It is a working principle diagram of a controller in the embodiment of the present application.

[0030] In the figure:

[0031] 100, freeze refrigerator; 110, controller;

[0032] 200, freeze box; 210, box body; 211, temperature sensor; 220, support ring; 230, cold collection seat; 231, freeze cavity; 232, air cooling cavity; 233, cold air inlet; 234, cold air outlet; 235, partition plate; 236, ventilation hole; 240, air supply mechanism; 241, first pressing air bag; 242, first driving component; 243, first air suction pipe; 244, first air exhaust pipe; 245, second pressing air bag; 246, second driving component; 247, second air suction pipe; 248, second air exhaust pipe; 250, air inlet pipeline; 251, air inlet guide pipe; 252, air inlet branch pipe; 253, air outlet solenoid valve rod; 260, air outlet pipeline; 261, air outlet guide pipe; 262, air outlet branch pipe; 263, air outlet solenoid valve rod; 270, freeze rack. DETAILED DESCRIPTION

[0033] Clearly, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0034] As shown in Figures 1 to 4 The present application provides a freeze storage device for infectious disease cell culture, which comprises a freeze storage refrigerator 100 and a freeze storage box 200 used in the freeze storage refrigerator 100. In fact, the freeze storage refrigerator 100 is a low-temperature refrigerator commonly used in laboratories for cell freezing, which can cool the test tube containing the infectious disease cell sample to -80℃ according to the set cooling sequence; and the present application further comprises a freeze storage box 200. Figure 1 In the actual application, the freeze storage volume in the freeze storage refrigerator 100 can be increased according to the requirement to place more freeze storage boxes 200.

[0035] The freeze storage box 200 comprises a box body 210, a supporting ring 220, a cold sink 230 and a blowing mechanism 240. The supporting ring 220 is arranged in the box body 210 to allow the lower part of the test tube containing the infectious disease cell sample to pass through and to support the upper part of the test tube. The cold sink 230 is arranged in the box body 210 and located directly below the supporting ring 220. The upper part of the cold sink 230 is provided with a freeze storage cavity 231 for the lower part of the test tube to extend into. In fact, the box body 210 can be made of plastic material and provided with an opening and closing cover. The box body 210 can be opened to place the test tube containing the infectious disease cell sample. After the test tube is placed, the box body 210 can be placed in the freeze storage space of the freeze storage refrigerator 100 together with the test tube. In the actual application, the head of the test tube used to contain the infectious disease cell sample is provided with a rubber seat larger than the outer diameter of the test tube, which facilitates the storage of the test tube and subsequent experiments and detection. In order to realize the supporting function of the supporting ring 220, the inner diameter of the supporting ring 220 is larger than the outer diameter of the test tube and smaller than the outer diameter of the rubber seat, so that the test tube can pass through the supporting ring 220 from top to bottom and the lower side of the rubber seat abuts against the upper side of the supporting ring 220, so that the supporting ring 220 can support the test tube. The cold sink 230 can be made of noble metal alloy material such as gold, silver, copper and platinum. The freeze storage cavity 231 is used for the lower part of the test tube, and the infectious disease cell sample is located in the lower part of the test tube, so that the freeze storage cavity 231 can surround the infectious disease cell sample. In the freeze storage process, the cold sink 230 first absorbs and emits heat, causing its temperature to drop. Subsequently, the heat around the cold sink 230 is gradually emitted and absorbed by the cold sink 230, so that the temperature in the test tube can uniformly decrease, and finally reaches the same temperature as the freeze storage space in the freeze storage refrigerator 100, so as to realize the freeze storage of the infectious disease cell sample in the test tube.

[0036] The box 210 is provided with a temperature sensor 211 placed in the freezing cavity 231, the air supply mechanism 240 is installed on the box 210 and used to blow the gas in the freezing refrigerator 100 to the lower part of the cooling seat 230, and the freezing refrigerator 100 is provided with a controller 110 in communication connection with the temperature sensor 211 and the air supply mechanism 240. In fact, the temperature sensor 211 can be a high-precision PT100 temperature sensor, which can be installed in the freezing cavity 231 or installed on the supporting ring 220 and extended into the freezing cavity 231 to detect the temperature of the freezing cavity 231, and provide reference data for the temperature in the freezing refrigerator 100 and the working state of the air supply mechanism 240; the air supply mechanism 240 can be a fan installed on the box 210, which can suck the gas in the freezing refrigerator 100 into the box 210 and blow it to the lower part of the cooling seat 230, and change the working state of the air supply mechanism 240, so as to change the cooling speed of the cooling seat 230 and further change the cooling temperature in the freezing cavity 231; the controller 110 can be a controller provided with the freezing refrigerator 100 and can be a programmable logic control unit, an industrial computer, a single board computer, a field bus control system, etc., which can read the temperature data of the temperature sensor 211 and the working data of the freezing refrigerator 100, and control the working state of the air supply mechanism 240 and the working state of the freezing refrigerator 100, and improve the automation degree of the freezing device of the application. In practical application, the box 210 can be positioned and connected with the freezing refrigerator 100 through a quick interface, so as to realize the communication connection between the controller 110 and the temperature sensor 211 and the air supply mechanism 240.

[0037] The specific working principle of the air supply mechanism 240 of the application is as follows: first, set the freezing temperature time curve of the freezing refrigerator 100 in the controller 110; if the actual temperature value of the temperature sensor 211 read by the controller 110 is less than the preset temperature value corresponding to the time on the freezing temperature time curve, the controller 110 controls the air supply mechanism 240 to stop working or reduce the air suction speed, and correspondingly the temperature of the freezing refrigerator 100 can also be raised as needed; if the actual temperature value of the temperature sensor 211 read by the controller 110 is greater than or equal to the preset temperature value corresponding to the time on the freezing temperature time curve, the controller 110 controls the air supply mechanism 240 to keep working or increase the air suction speed, and correspondingly the temperature of the freezing refrigerator 100 can also be raised as needed.

[0038] The freezing device for infectious disease cell culture of the application controls the working state of the air supply mechanism 240 to control the cooling speed of the cooling seat 230, so that the temperature time curve in the freezing cavity 231 is closer to the preset temperature time curve, and the freezing effect of the infectious disease cell culture is ensured.

[0039] Specifically, as Figure 3As shown, the lower part of the condensing seat 230 is preferably provided with a wind cooling cavity 232, a wind cooling inlet 233 communicating with the wind cooling cavity 232, and a wind cooling outlet 234 communicating with the wind cooling cavity 232. The box body 210 is provided with an air inlet pipeline 250 communicating the outside of the box body 210 with the wind cooling inlet 233, and an air outlet pipeline 260 communicating the outside of the box body 210 with the wind cooling outlet 234. The air supply mechanism 240 is used to drive the gas in the cryogenic refrigerator 100 to pass through the air inlet pipeline 250, the wind cooling cavity 232, and the air outlet pipeline 260 in sequence. In fact, when the air supply mechanism 240 is working, the gas in the cryogenic refrigerator 100 enters the air inlet pipeline 250, then enters the wind cooling cavity 232 to exchange heat with the condensing seat 230, and finally leaves the wind cooling cavity 232 through the air outlet pipeline 260 and returns to the cryogenic refrigerator 100 again, reducing the contact between the gas drawn into the box body 210 by the air supply mechanism 240 and other parts of the box body 210, ensuring that the gas blown by the air supply mechanism 240 contacts the lower part of the condensing seat 230 to ensure the heat exchange effect, and achieving better control of the temperature of the condensing seat 230.

[0040] More specifically, the wind cooling inlet 233 communicates with the lower left part of the wind cooling cavity 232, and the wind cooling outlet 234 communicates with the lower right part of the wind cooling cavity 232. The wind cooling cavity 232 is preferably provided with two parallel partitions 235 inclined downward from left to right. The lower end of the partition 235 near the wind cooling inlet 233 and the upper end of the partition 235 near the wind cooling outlet 234 are both provided with air vents 236. In fact, the partitions 235 and the condensing seat 230 are integrally formed of the same material. Figure 3 As shown, the gas in the cryogenic refrigerator 100 enters the wind cooling inlet 233 from the air inlet pipeline 250 under the action of the air supply mechanism 240, passes through the lower air vents 236 to enter between the two partitions 235, and then passes through the upper air vents 236 to enter the wind cooling outlet 234. The whole process involves multiple turns, which prolongs the residence time of the gas in the wind cooling cavity 232, to ensure the heat transfer effect of the gas and the condensing seat 230.

[0041] Further, the freezing box 200 also preferably comprises a freezing rack 270 placed in the box body 210, the freezing rack 270 is provided with a plurality of freezing rings, a plurality of the cold seats 230 are correspondingly arranged below the freezing rings, and a plurality of the temperature sensors 211 are correspondingly inserted into the freezing cavities 231. In fact, the freezing rings are integrally formed with the freezing rack 270 and are positioned in the box body 210 so that the plurality of cold seats 230 are correspondingly arranged below the freezing rings. By correspondingly arranging the plurality of cold seats 230, the plurality of freezing rings and the plurality of temperature sensors 211, a plurality of test tubes can be simultaneously frozen, thereby improving the freezing efficiency of infectious disease cells. Figure 2 In the drawings, there are two cold seats 230, and in actual application, the number of cold seats can be increased or decreased according to the corresponding requirements.

[0042] Further, the air inlet pipeline 250 preferably comprises an air inlet guide pipe 251 and a plurality of air inlet branch pipes 252 in communication with the air inlet guide pipe 251 and each provided with an air inlet magnetic valve rod 253, and the plurality of air inlet branch pipes 252 are in communication with the air cooling inlets 233 of the plurality of cold seats 230; the air outlet pipeline 260 comprises an air outlet guide pipe 261 and a plurality of air outlet branch pipes 262 in communication with the air outlet guide pipe 261 and each provided with an air outlet magnetic valve rod 263, and the plurality of air outlet branch pipes 262 are in communication with the air cooling inlets 233 of the plurality of cold seats 230; the air inlet magnetic valve rod 253 and the air outlet magnetic valve rod 263 are electrically connected with the controller 110. In fact, the air supply mechanism 240 realizes the diversion of the gas entering the air inlet pipeline 250 to the air cooling cavities 232 of the plurality of cold seats 230 through the air inlet guide pipe 251 and the plurality of air inlet branch pipes 252, and the air supply mechanism 240 realizes the convergence of the gas actually leaving the air cooling cavities 232 of the plurality of cold seats 230 and leaving through the air outlet guide pipe 261 and the plurality of air outlet branch pipes, thereby ensuring that the air supply mechanism 240 can act on the plurality of cold seats 230. It is worth mentioning that by means of the air inlet magnetic valve rod 253 and the air outlet magnetic valve rod 263, it is possible to respectively control whether the corresponding air cooling is connected to the gas drawn by the air supply mechanism 240, so as to respectively control the cooling speed of the plurality of cold seats 230, thereby realizing the temperature control of the plurality of freezing cavities.

[0043] It should be noted that, as Figure 2As shown, the air supply mechanism 240 preferably includes a first pressing airbag 241 and a first driving component 242 for driving the first pressing airbag 241 to expand or contract. The first pressing airbag 241 is provided with a first inhalation tube 243 placed inside the freezer 100 and a first exhalation tube 244 connected to the air inlet duct 251. The first driving component 242 is connected to the controller 110. In practice, the first inhalation tube 243 and the first exhalation tube 244 of the first pressing airbag 241 are both one-way air tubes or are provided with one-way valves, so that the first inhalation tube 243 can only introduce gas into the first pressing airbag 241 and the first exhalation tube 244 can only expel gas from the first pressing airbag 241. The first driving component 242 can be an electrically telescopic rod, which drives the first pressing airbag 241 to increase or decrease in volume. During operation, the first driving component 242 retracts to inflate the first pressing airbag 241. The first pressing airbag 241 draws in air from the outside of the housing 210 through the first suction tube 243. The first driving component 242 extends to compress the first pressing airbag 241. The first pressing airbag 241 then passes air into the air inlet duct 251 through the first exhalation tube 244, thus realizing the air delivery function of the air delivery mechanism 240. Furthermore, by changing the inflation and deflation frequency of the first pressing airbag 241 driven by the first driving component 242, the speed at which the air delivery mechanism 240 delivers air into the air inlet duct 251 is changed.

[0044] Furthermore, the air supply mechanism 240 preferably includes a second pressing airbag 245 and a second driving component 246 for driving the second pressing airbag 245 to expand or contract. The second pressing airbag 245 is provided with a second inhalation tube 247 connected to the air outlet duct 261 and a second exhalation tube 248 placed inside the freezer 100. The second driving component 246 is connected to the controller 110. In practice, the second inhalation tube 247 and the second exhalation tube 248 of the second pressing airbag 245 are both one-way air tubes or are equipped with one-way valves, so that the second inhalation tube 247 can only introduce gas into the second pressing airbag 245 and the second exhalation tube 248 can only expel gas from the second pressing airbag 245. The second driving component 246 can be an electrically telescopic rod, which drives the second pressing airbag 245 to increase or decrease in volume. During operation, the second driving component 246 retracts, causing the second pressing airbag 245 to expand. The second pressing airbag 245 draws in gas through the second suction tube 247 from the air outlet duct 261. The second driving component 246 extends, causing the second pressing airbag 245 to compress. The second pressing airbag 245 then discharges gas outwards from the housing 210 through the second exhalation tube 248, thus realizing the air delivery function of the air delivery mechanism 240. Furthermore, by changing the expansion and contraction frequency of the second pressing airbag 245 driven by the second driving component 246, the speed at which the air delivery mechanism 240 introduces gas into the air inlet duct 251 is changed. It is worth mentioning that the first pressing airbag 241 and the second pressing airbag 245 cooperate with each other to improve the air delivery capacity of the air delivery mechanism 240. Furthermore, by changing the time interval between the actions of the first driving component 242 and the second driving component 246, the first pressing airbag 241 and the second pressing airbag 245 can mutually enhance or weaken each other, making the air delivery capacity range that the air delivery mechanism 240 can adjust larger, and providing a more suitable and controllable cooling rate for the cooling seat 230.

[0045] Furthermore, the present invention also provides a method for cryopreserving infectious disease cell cultures, using the above-mentioned cryopreservation device for infectious disease cell cultures, the cryopreservation method comprising the following steps:

[0046] S1, Set the freezing temperature-time curve of the freezer 100 in the controller 110;

[0047] S2, Place the test tube containing the infectious disease cell sample into the cryopreservation box 200, so that the lower part of the test tube passes through the support ring 220 and extends into the cryopreservation chamber 231;

[0048] S3, place the cryopreservation box 200 containing test tubes into the cryopreservation refrigerator 100, and make the temperature sensor 211 and the air supply mechanism 240 communicate with the controller 110.

[0049] S4, the controller 110 controls the air supply mechanism 240 to work according to the detection value of the temperature sensor 211.

[0050] The present invention provides a cryopreservation method for infectious disease cell culture. By controlling the working state of the air supply mechanism 240, the cooling rate of the cooling seat 230 is controlled, so that the temperature-time curve in the cryopreservation chamber 231 has a smaller difference from the preset temperature-time curve, thereby ensuring the cryopreservation effect of infectious disease cell culture.

[0051] In step S4, the controller 110 controls the operation of the air supply mechanism preferably in the following two ways.

[0052] The first method, in step S4:

[0053] If the controller 110 reads that the actual temperature value of the temperature sensor 211 is less than the preset temperature value at the corresponding time on the freezing temperature-time curve, the controller 110 controls the air supply mechanism 240 to stop working.

[0054] If the controller 110 reads that the actual temperature value of the temperature sensor 211 is greater than or equal to the preset temperature value at the corresponding time on the freezing temperature-time curve, the controller 110 controls the air supply mechanism 240 to keep working.

[0055] That is, by controlling the start and stop of the working state of the air supply mechanism 240, the temperature of the cooling base 230 can be controlled to determine whether the cooling base 230 continues to cool down.

[0056] The second method, in step S4:

[0057] If the controller 110 reads that the actual temperature value of the temperature sensor 211 is less than the preset temperature value at the corresponding time on the freezing temperature-time curve, the controller 110 controls the air supply mechanism 240 to reduce its operating frequency.

[0058] If the controller 110 reads that the actual temperature value of the temperature sensor 211 is greater than or equal to the preset temperature value at the corresponding time on the freezing temperature-time curve, the controller 110 controls the air supply mechanism 240 to increase its operating frequency.

[0059] That is, by controlling the working frequency of the air supply mechanism 240, the air volume blown towards the cooling base 230 is controlled, thereby controlling the temperature of the cooling base, which is more flexible than the first method.

[0060] The above are merely specific embodiments of the present invention and do not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A cryopreservation device for infectious disease cell culture, characterized in that, The invention includes a cryopreservation refrigerator and a cryopreservation box placed inside the cryopreservation refrigerator. The cryopreservation box includes a box body, a support ring, a cold-collecting base, and an air supply mechanism. The support ring is placed inside the box body to allow the lower part of a test tube containing infectious disease cell samples to pass through and to support the upper part of the test tube. The cold-collecting base is placed inside the box body and located directly below the support ring. The upper part of the cold-collecting base has a cryopreservation cavity for the lower part of the test tube to extend into. The box body has a temperature sensor placed inside the cryopreservation cavity. The air supply mechanism is installed on the box body and is used to blow gas from inside the cryopreservation refrigerator toward the lower part of the cold-collecting base. The cryopreservation refrigerator has a controller for communicating with the temperature sensor and the air supply mechanism.

2. The cryopreservation device for infectious disease cell culture as described in claim 1, characterized in that, The lower part of the cooling base is provided with an air-cooled cavity, an air-cooled inlet connected to the air-cooled cavity, and an air-cooled outlet connected to the air-cooled cavity. The box body is provided with an air inlet pipe connecting the outside of the box body to the air-cooled inlet and an air outlet pipe connecting the outside of the box body to the air-cooled outlet. The air supply mechanism is used to drive the gas inside the freezer to pass through the air inlet pipe, the air-cooled cavity, and the air outlet pipe in sequence.

3. The cryopreservation device for infectious disease cell culture as described in claim 2, characterized in that, The air-cooling inlet is connected to the lower left part of the air-cooling cavity, and the air-cooling outlet is connected to the lower right part of the air-cooling cavity. The air-cooling cavity is provided with two parallel partitions that are inclined downwards from left to right. Ventilation holes are provided at the connection between the lower end of the partition near the air-cooling inlet and the air-cooling cavity, and at the connection between the upper end of the partition near the air-cooling outlet and the air-cooling cavity.

4. The cryopreservation device for infectious disease cell culture as described in claim 2, characterized in that, The cryopreservation box also includes a cryopreservation rack placed inside the box. The cryopreservation rack is provided with multiple cryopreservation rings, and multiple cold-collecting seats are respectively arranged directly below the multiple cryopreservation rings. Multiple temperature sensors are respectively inserted into multiple cryopreservation chambers.

5. The cryopreservation device for infectious disease cell culture as described in claim 4, characterized in that, The air inlet duct includes an air inlet duct and multiple air inlet branch pipes connected to the air inlet duct, each equipped with an air inlet solenoid valve rod. The multiple air inlet branch pipes are connected to the air-cooled inlets of multiple cooling bases. The air outlet duct includes an air outlet duct and multiple air outlet branch pipes connected to the air outlet duct, each equipped with an air outlet solenoid valve rod. The multiple air outlet branch pipes are connected to the air-cooled inlets of multiple cooling bases. Both the air inlet solenoid valve rod and the air outlet solenoid valve rod are electrically connected to the controller.

6. The cryopreservation device for infectious disease cell culture as described in claim 5, characterized in that, The air supply mechanism includes a first pressing airbag and a first driving component for driving the first pressing airbag to expand or contract. The first pressing airbag is provided with a first inhalation tube placed inside a freezer and a first exhalation tube connected to an air inlet duct. The first driving component is connected to a controller.

7. The cryopreservation device for infectious disease cell culture as described in claim 6, characterized in that, The air supply mechanism includes a second pressing airbag and a second driving component for driving the second pressing airbag to expand or contract. The second pressing airbag is provided with a second inhalation tube that is connected to the air outlet duct and a second exhalation tube that is placed inside the freezer. The second driving component is connected to the controller.

8. A method for cryopreservation of infectious disease cell cultures, characterized in that, The cryopreservation apparatus for infectious disease cell culture as described in any one of claims 1-7, wherein the cryopreservation method comprises the following steps: S1, Set the freezing temperature-time curve of the freezer in the controller; S2, Place the test tube containing the infectious disease cell sample into the cryopreservation box, so that the lower part of the test tube passes through the support ring and extends into the cryopreservation chamber; S3, place the cryopreservation box containing the test tubes into the cryopreservation refrigerator, and connect the temperature sensor and the air supply mechanism to the controller. S4, the controller controls the air supply mechanism to work according to the detection value of the temperature sensor.

9. The cryopreservation method for infectious disease cell culture as described in claim 8, characterized in that, In step S4: If the controller reads that the actual temperature value of the temperature sensor is less than the preset temperature value at the corresponding time on the freezing temperature-time curve, the controller will control the air supply mechanism to stop working. If the controller reads that the actual temperature value of the temperature sensor is greater than or equal to the preset temperature value at the corresponding time on the freezing temperature-time curve, the controller will control the air supply mechanism to continue working.

10. The cryopreservation method for infectious disease cell culture as described in claim 8, characterized in that, In step S4: If the controller reads that the actual temperature value of the temperature sensor is less than the preset temperature value at the corresponding time on the freezing temperature-time curve, the controller will control the air supply mechanism to reduce its operating frequency. If the controller reads that the actual temperature value of the temperature sensor is greater than or equal to the preset temperature value at the corresponding time on the freezing temperature-time curve, the controller will control the air supply mechanism to increase its operating frequency.

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