Method for promoting celery growth and improving stress tolerance of celery through light regulation and CO2 treatment
By regulating photoperiod and enriching CO2, the problem of poor resistance of celery to high temperature stress was solved, promoting celery growth and improving its resistance to high temperature, as well as enhancing photosynthetic capacity and antioxidant enzyme activity.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-02-12
- Publication Date
- 2026-03-31
AI Technical Summary
Celery has poor resistance to high temperature stress, which affects its growth and yield.
The treatment involved photoperiod regulation and CO2 enrichment. Specifically, during the rapid growth period of celery (45-85 days), CO2 enrichment was carried out for 25-30 days at a concentration of 800 ppm. The photoperiod was 12h/12h, the light intensity was 15400 lx, and the CO2 enrichment time was 4 hours after the start of the daily photoperiod.
It significantly promotes celery growth, enhances its resistance to high temperature stress, strengthens photosynthetic capacity and antioxidant enzyme activity, reduces stomatal conductance, and improves celery's resistance to abiotic stress.
Smart Images
Figure CN121753665A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of high-efficiency plant cultivation technology, and in particular to a method for promoting celery growth and improving celery stress resistance through light regulation and CO2 treatment. Background Technology
[0002] Celery (Apium graveolens L.) is an annual or perennial herbaceous plant belonging to the Apiaceae family. It is a very important vegetable crop, originally from the marshy areas of the Mediterranean coast, and is widely cultivated and consumed worldwide. Celery is a semi-hardy vegetable, intolerant of heat. The optimal temperature for seed germination is 15–20℃, and the optimal temperature for plant growth is 15–25℃. It will not grow well in environments above 26℃, and is best cultivated and planted in spring and autumn.
[0003] Many biotic and abiotic stresses affect plant growth and development, thus impacting yield, such as drought, low temperature, high temperature, soil salinization, and pests and diseases. High temperature stress is a significant factor affecting celery growth and reducing yield. Therefore, promoting celery growth and improving its resistance to high temperature stress to increase yield is a key research focus for those skilled in the art. Summary of the Invention
[0004] The purpose of this invention is to provide a method for promoting celery growth and improving celery stress resistance through light regulation and CO2 treatment, so as to solve the problems existing in the prior art.
[0005] To achieve the above objectives, the present invention provides the following solution:
[0006] The technical solution of this invention: A method for promoting celery growth and improving celery stress resistance through light regulation and CO2 treatment, comprising the following steps:
[0007] When the celery grows to the "four leaves and one heart" stage, the photoperiod of the celery is regulated until the celery is harvested. The photoperiod is controlled to be 12h / 12h (i.e., 12h light period and 12h dark period). During the rapid growth period of the celery, the celery is treated with CO2 enrichment for 25-30 days (d).
[0008] A 12h / 12h (D / N) photoperiod can significantly improve chlorophyll content, photosynthetic electron transport efficiency, and gas exchange capacity in celery by balancing the metabolic rhythms of photosynthesis and the transport and accumulation of substances during the dark period. This, in turn, promotes plant height, biomass, leaf area, and root development, while optimizing nutrient quality. This photoperiod setting avoids photoinhibition, reactive oxygen species accumulation, and damage to photosynthetic structures caused by excessively long light exposure, and reduces the risk of bolting induced by long days. By adapting to the endogenous biological clock of celery, stabilizing physiological metabolism, and optimizing photosynthesis and substance distribution, it enhances plant stress resistance by protecting the photosynthetic system, reducing physiological disorders, and delaying the transition to reproductive growth, thus achieving a synergistic improvement in growth and stress resistance.
[0009] CO2 is a core substrate for celery photosynthesis and enhances celery's tolerance to stress through multiple physiological and biochemical pathways. Regarding celery growth promotion, CO2 binds to RuBisCO enzymes in chloroplasts and enters the Calvin cycle, synthesizing carbohydrates to provide energy for root, stem, and leaf growth. Appropriately increasing its concentration can improve photosynthetic rate and biomass, thereby promoting celery growth. Regarding stress resistance, CO2 can reduce stomatal conductance and transpiration, increase antioxidant enzyme activity and scavenge reactive oxygen species, thus improving celery's resistance to abiotic stress (high temperature stress). This invention achieves a synergistic effect of optimizing celery growth and development and enhancing stress resistance by subjecting celery to 25-30 days of CO2 enrichment treatment during its rapid growth period.
[0010] Celery exhibits extremely high photosynthetic activity and carbon requirements during its rapid growth phase. Providing additional CO2 at this time maximizes its use in carbon assimilation, thereby maximizing biomass accumulation. The rapid growth phase of celery typically occurs between 45 and 85 days of physiological seedling age, influenced by variety, temperature, light, and water and fertilizer management. During this stage, celery plants are no longer limited to single-leaf growth but enter a synergistic developmental period characterized by rapid petiole elongation, a continuous increase in the number of leaves, and synchronous expansion of the underground root system—the core stage for yield formation. Enriching celery with CO2 at this time not only maximizes its high photosynthetic potential but also enhances its resistance to abiotic stresses by regulating endogenous hormone balance and the antioxidant system.
[0011] Furthermore, the rapid growth period of celery specifically refers to celery seedlings aged 45-85 days (during which CO2 enrichment treatment is carried out for 25-30 consecutive days). This varies slightly depending on the celery variety.
[0012] Furthermore, the CO2 concentration in the CO2 enrichment treatment is 800 ppm.
[0013] The inventors discovered that this concentration falls within the optimal range for celery's photosynthetic response. On the one hand, this concentration is close to or reaches the CO2 saturation point of celery, effectively increasing the photosynthetic rate without wasting resources; on the other hand, it avoids problems such as excessive decrease in stomatal conductance, limited transpiration, or carbon metabolism feedback inhibition that may occur with higher concentrations (e.g., >1200 ppm).
[0014] Furthermore, the CO2 enrichment treatment is performed once a day (for 25-30 consecutive days, i.e., 25-30 consecutive times), starting 1 hour after the daily photocycle and lasting for 4 hours (i.e., CO2 is applied 1 hour after the light exposure to bring the CO2 concentration in the incubator environment to 800 ppm and maintain this concentration for 4 hours).
[0015] Furthermore, the illuminance during the photoperiod is 15400 lx.
[0016] Furthermore, the celery in question is Ventura celery.
[0017] Preferably, when the Ventura celery grows to the "four leaves and one heart" stage, the photoperiod of the celery is regulated until the celery is harvested, and the photoperiod is controlled to be 12h / 12h. The Ventura celery is also subjected to CO2 enrichment treatment for 25 days when the seedlings are 61-85 days old (rapid growth period).
[0018] Furthermore, the improvement of celery stress resistance specifically refers to improving celery's resistance to high-temperature stress.
[0019] The present invention discloses the following technical effects:
[0020] This invention sets the photoperiod of celery growth to 12h / 12h, which can effectively promote celery growth.
[0021] This invention utilizes a CO2 enrichment environment of 800 ppm for 25-30 days during the rapid growth period of celery, which can effectively promote celery growth and improve celery's resistance to high temperature stress.
[0022] This invention tested the changes in the physiological and biochemical characteristics of celery after CO2 enrichment treatment and under high-temperature stress, demonstrating that the method has a positive effect on promoting celery growth and enhancing its resistance to abiotic stress (high-temperature stress). This proves that the method of this invention has broad application prospects. Attached Figure Description
[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0024] Figure 1 The results of growth observation and physiological index testing of celery seedlings under different photoperiods after photoperiod screening are shown. Among them, a is CAT enzyme activity, b is SOD enzyme activity, c is net photosynthetic rate, d is chlorophyll b content in leaves, e is chlorophyll a content in leaves, and f is plant height.
[0025] Figure 2 These are phenotypic images of the EC and CK groups before and after high-temperature treatment.
[0026] Figure 3 The results validate the plant growth characteristics of the EC and CK groups.
[0027] Figure 4 The results show the photosynthetic characteristics of the EC and CK groups before and after high-temperature treatment.
[0028] Figure 5 The results show the chlorophyll fluorescence characteristics of the EC and CK groups before and after high-temperature treatment.
[0029] Figure 6 The results show the chlorophyll content of the EC and CK groups before and after high-temperature treatment.
[0030] Figure 7 The results of antioxidant enzyme activity, osmotic regulation capacity, and cell membrane damage tests in the EC and CK groups before and after high-temperature treatment are shown. Among them, a is proline content, b is MDA content, c is CAT enzyme activity, d is POD enzyme activity, and e is SOD enzyme activity. Detailed Implementation
[0031] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.
[0032] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.
[0033] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.
[0034] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be obvious to those skilled in the art. This specification and embodiments are merely exemplary.
[0035] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.
[0036] It should be noted that any aspects not described in detail in this invention are conventional practices in the field and are not the focus of this invention.
[0037] In the following embodiments of the present invention, room temperature specifically refers to 20-30 ℃.
[0038] All raw materials used in the following embodiments of the present invention are commercially available products.
[0039] Example 1
[0040] Celery cultivation experiment
[0041] 1. Test site and varieties
[0042] This example was conducted from August to November 2025 in the greenhouse and artificial climate chamber of the College of Horticulture, Sichuan Agricultural University, where the 'Ventura' celery variety was cultivated. Celery seeds were soaked in 50℃ warm water for 30 minutes, then removed and soaked in cool water (room temperature water) for 24 hours. They were then placed in a seed culture box for germination (temperature 18℃, humidity 80%, keeping the gauze moist) for 7 days. After 7 days, the germinated seeds were sown into seedling trays (50 cells each) and placed in the culture box for seedling cultivation.
[0043] 2. Experimental Design and Methods
[0044] Photoperiod selection: When seedlings reached the "four leaves and one bud" stage, healthy seedlings with uniform growth were transferred to nutrient pots (14cm×16cm) (the substrate was a mixture of nutrient soil, perlite, and vermiculite in a 3:1:1 mass ratio) and placed in an artificial climate chamber to better control the seedling growth environment. Under conditions of 23 / 15℃ (D / N, i.e., 23℃ during the photoperiod and 15℃ during the dark period) and 80% humidity, six different photoperiod treatments were established, with the photoperiod / dark period (D / N) ratios for each group as follows: 6h / 18h (PP1), 8h / 16h (PP2), 10h / 14h (PP3), 12h / 12h (PP4), 14h / 10h (PP5), and 16h / 8h (CK0, i.e., control group). The light intensity during the photoperiod was 15400 lx for each group. When the seedlings reach 60 days old, samples are taken immediately for growth observation and physiological index measurement to screen out the most suitable photoperiod conditions for celery growth.
[0045] CO2 enrichment and high-temperature treatment: After selecting the treatment group with the best growth and various indicators by comparing growth and other indicators, subsequent treatments were immediately carried out. The celery seedlings in this group (later designated PP4 group) were divided into two groups of 20 seedlings each. One group received CO2 enrichment treatment (designated EC group), while the other group did not receive CO2 enrichment treatment and grew entirely under atmospheric CO2 concentration (tested to be approximately 400 ppm), serving as the CK control group. During the CO2 enrichment treatment phase, all treatment conditions were identical except for the CO2 concentration. The photoperiod was maintained at 12h / 12h for both groups, and temperature, humidity, and light intensity remained consistent with the photoperiod selection period. The EC group underwent a 4-hour CO2 enrichment treatment (introducing CO2 to bring the CO2 concentration in the environment of this group of celery seedlings to 800 ppm) 1 hour after the start of the daily photoperiod. After 25 consecutive days of CO2 enrichment treatment (i.e., seedling age 61-85 days), samples were taken from both the CK and EC groups for growth and physiological index testing. Ten celery plants from each group were then subjected to high-temperature treatment in an artificial climate chamber at 39 / 31℃, with all other growth conditions remaining the same as before the high-temperature treatment. After 36 hours of high-temperature treatment, samples were taken from both groups (denoted as CKH and ECH) for growth and physiological index measurement.
[0046] 3. Measurement Items and Methods
[0047] Three healthy plants were randomly selected from each group for indicator measurement.
[0048] (1) Measurement of growth indicators:
[0049] Plant height: Measure the height from the base of the stem to the seedling growth point using a measuring tape.
[0050] Spread width: Measure the width of the leaf cluster at its widest point using a soft measuring tape.
[0051] Fresh weight of above-ground / underground parts: After rinsing the plant with deionized water, blot dry with absorbent paper and weigh the fresh weight using a balance.
[0052] Aboveground / underground dry weight: Wrap the fresh sample in newspaper and place it in an oven at 60°C until constant weight is reached (the standard for drying is that the relative error of the mass of the last two weighings is <0.5%), and then weigh the dry weight.
[0053] (2) Measurement of physiological indicators:
[0054] 1) Photosynthetic parameters:
[0055] Select the 3rd and 4th functional leaves of celery. One day before the measurement, place the plants under natural light for 24 hours to acclimatize, avoiding fluctuations in photosynthetic parameters due to sudden environmental changes. Use a LI-6400 portable photosynthesis meter (LI-COR, USA). After instrument calibration, measurements were taken between 9:00 and 11:00 AM, avoiding the celery's "photosynthetic midday rest" period. Lay the leaves flat in the red and blue light source leaf chamber, ensuring the leaf chamber completely covers the marked area, and the leaves are without wrinkles or overlaps. Close the leaf chamber and secure it tightly. Turn on the light source in the leaf chamber and allow the leaves to acclimatize under the set light intensity for 3-5 minutes. Observe the values on the instrument display. When the value fluctuation is ≤5% within 30 seconds, it is considered stable. Read and record the values directly (including transpiration rate, net photosynthetic rate, stomatal conductance, and intercellular CO2 concentration).
[0056] 2) Fluorescence parameters:
[0057] Celery plants with uniform growth were selected, with the upper and middle functional leaves as the measurement subjects. Chlorophyll fluorescence parameters of the celery were measured using a PAM-2500 portable modulated chlorophyll fluorometer (Walz GmbH, Germany). After instrument calibration, the leaves underwent dark adaptation treatment. Using the instrument's matching dark adaptation clip, the leaf was fixed to the test area 15-20 minutes before measurement. The ambient temperature was kept stable to ensure the leaf's physiological state was not affected by external environmental fluctuations. After dark adaptation, weak measurement light was applied first to measure Fo (initial fluorescence / minimum fluorescence), followed by one saturated pulse light, recording Fm (maximum fluorescence). Then, the dark adaptation clip was replaced with a light adaptation leaf clip, and photochemical light was applied to irradiate the leaves for 3-5 minutes until the fluorescence signal reached a steady state, recording data such as Fs (steady-state fluorescence). Saturated pulse light was then applied again, recording the maximum fluorescence (Fm') under light adaptation. Immediately after the photochemical light was turned off, far-red light was applied, recording the minimum fluorescence (Fo') and photosynthetically active radiation (PAR) under light adaptation. Calculate the maximum photochemical efficiency ΦPSⅡ, specifically, ΦPSⅡ (%) = [(Fm - Fo) / Fm]. Calculate the electron transport rate ETR, specifically, ETR (μmol·e·m).-2 ·s -1 =ΦPSⅡ×PAR×0.5×0.84. Calculate the variable fluorescence Fv, specifically, Fv=Fm-Fo. Calculate the photochemical quenching coefficient qP, specifically qP,=[(Fm'-Fs) / (Fm'-Fo')]. Calculate the non-photochemical quenching coefficient NPQ, specifically NPQ=(Fm-Fm') / Fm.
[0058] 3) Photosynthetic pigments:
[0059] The photosynthetic pigment content was extracted using an acetone-ethanol (1:1 volume ratio) mixture method. Fresh celery leaves or petioles were mixed thoroughly, and 0.2 g (depending on chlorophyll content) was immediately and accurately weighed into a 10 mL centrifuge tube. 10 mL of the acetone-ethanol mixture was quickly added, the tube was capped, and the mixture was shaken well and placed in the dark for 24 hours. The supernatant was then measured using a spectrophotometer at wavelengths of 663 nm, 645 nm, and 652 nm to determine the optical density of the pigment solution and calculate the corresponding pigment content.
[0060] 4) Antioxidant enzyme activity:
[0061] a) SOD (superoxide dismutase) was reacted using the nitroblue tetrazolium (NBT) method. Weigh 0.5g of fresh sample, add 1mL of pre-cooled phosphate buffer (0.05mol / L, pH=7.8) and a small amount of quartz sand, grind into a paste on ice, add another 1mL of buffer, pour into a centrifuge tube, rinse the mortar with 2mL of buffer, pour into the centrifuge tube, and bring the volume to 5mL. Centrifuge at low temperature (0-4℃) for 20min (3000rpm), carefully aspirate the supernatant and refrigerate. Take identical test tubes, add 100μL of supernatant to each tube (add 100μL of phosphate buffer to each of the two control tubes), add 3mL of reaction solution to each, place one control tube in the dark, and react the remaining tubes under 4000lx sunlight (artificial climate chamber) for 20-30min (ensuring consistent light exposure for all tubes; higher temperature shortens the reaction time, lower temperature lengthens the reaction time). After the reaction was completed, a blank control tube without light was used, and the absorbance value was measured at 560 nm.
[0062] b) POD (peroxidase) was measured using the guaiacol method. 0.5 g of sample was added to 5 mL of pre-chilled phosphate buffer (0.05 mol / L, pH 7.8) and centrifuged at low temperature (0-4℃) for 20 min (3000 rpm). The supernatant was carefully aspirated and stored under cold conditions. 100 μL of the supernatant was added to a cuvette (100 μL of phosphate buffer was added for the control), followed by 3 mL of reaction solution. The OD value at 470 nm was immediately read and timed, with readings every 30 seconds for 3 min.
[0063] c) The CAT (catalase) assay was performed using the guaiacol method. 0.5 g of sample was added to 5 mL of pre-cooled phosphate buffer (0.05 mol / L, pH 7.8) and centrifuged at low temperature (0-4℃) for 20 min (3000 rpm). The supernatant was carefully aspirated and stored under cold conditions. 100 μL of the supernatant was added to a cuvette (100 μL of phosphate buffer was added for the control), followed by 3 mL of reaction solution. The OD value at 240 nm was immediately read and timed, with readings taken every 1 min (OD values at 0, 1, 2, and 3 min).
[0064] 5) MDA (malondialdehyde) content:
[0065] Weigh 20g of trichloroacetic acid (TCA), dissolve it initially, transfer it to a volumetric flask, and dilute to 100mL with distilled water to prepare a TCA solution. Add 0.5g of thiobarbituric acid (TAB) to the TCA solution and dilute to 100mL to prepare a TAB solution. Take the third leaf from the growing point downwards, remove the main vein, weigh 0.2g, and grind it in an ice bath. Add 5mL of TAB solution. Stopper the mixture and extract in a boiling water bath, then filter to obtain the extract. Observe the absorbance of the extract at 450nm, 532nm, and 600nm (denoted as A). 450 A 532 and A 600 Calculate the MDA content using the following formula:
[0066] MDA content = [6.452 (A)] 532 -A 600 -0.56A 450 ]×V 总 ÷ (W×V) 测 );
[0067] In the formula, V 总 V represents the total volume of the extract (mL). 测 The volume of extract (mL) is used to determine the volume of extract taken; W is the sample weight (g).
[0068] 6) Proline content (acidic stigmine method):
[0069] Prepare a 200 μg / mL standard proline solution. Dissolve 1.5 g of sulfosalicylic acid in 50 mL of distilled water to prepare a sulfosalicylic acid solution. Dissolve 16 mL of H3PO4 in 50 mL of distilled water to prepare a phosphoric acid solution. Add 1.25 g of acidic phorate to 30 mL of glacial acetic acid and 20 mL of the prepared phosphoric acid solution to prepare an acidic phorate solution. Take the second leaf from the growing point downwards, remove the main vein, weigh 0.2 g, and grind in an ice bath. Add 5 mL of sulfosalicylic acid solution, stopper, extract in a water bath, and filter to obtain the extract. Dilute with standard proline solution to prepare standard curve solutions with concentration gradients of 0, 10, 20, 40, and 80 μg / mL. Take 2 mL of each gradient standard curve solution and the extract, add 2 mL of glacial acetic acid and 3 mL of acidic phorate solution respectively, boil in a water bath for 30 min, and measure the absorbance at 520 nm. Calculate the proline content according to the following formula:
[0070] Proline content = X × V 总 ÷ (W×V) 测 );
[0071] In the formula, X represents the proline content (μg) on the standard curve, and V 总 V represents the total volume of the extract (mL). 测 To determine the volume (mL) of extract taken, W is the sample weight (g).
[0072] Experimental results:
[0073] (1) The results obtained from observing the growth and testing the physiological indicators of celery seedlings under different photoperiods after photoperiod screening are as follows: Figure 1 As shown in the figure, a represents CAT enzyme activity, b represents SOD enzyme activity, c represents net photosynthetic rate, d represents chlorophyll b content in leaves, e represents chlorophyll a content in leaves, and f represents plant height. It can be seen that the PP4 group exhibited the best overall growth and physiological indicators.
[0074] (2) Growth phenotypes of EC group and CK group before and after high temperature treatment (e.g. Figure 2 As shown in the figure, the observations showed that before heat stress, there was no significant difference in the growth status between the CK group (control group plants) and the EC group (treatment group plants); after heat stress, the growth status and phenotype of the EC group plants were significantly better than those of the CK group, specifically, the leaves were less wilted and the overall plant growth was more robust, indicating that the treatment method corresponding to the EC group can effectively improve the growth status of plants under heat stress.
[0075] (3) Figure 3The results validated the growth characteristics (growth indicators) of the EC and CK groups, clearly showing the differences in specific biological characteristics between the CK and EC groups. This intuitively reflects the differences in the potential tolerance-related characteristics of the two groups of plants to high temperature stress, providing phenotypic evidence for subsequent indicator analysis.
[0076] (4) Figure 4 The results of the photosynthetic characteristics analysis of the EC and CK groups before and after high temperature treatment showed that before high temperature treatment, there was no significant difference in net photosynthetic rate and transpiration rate between the CK and EC groups. After high temperature stress (CKH was the control group and ECH was the treatment group), the net photosynthetic rate of the ECH group was significantly higher than that of the CKH group, and the transpiration rate remained at a more stable and reasonable level, indicating that the treatment corresponding to the EC group can effectively alleviate the inhibition of photosynthesis and transpiration of plants by high temperature.
[0077] (5) The results of chlorophyll fluorescence characteristics detection in the EC group and the CK group before and after high temperature treatment are as follows: Figure 5 As shown, under high temperature stress, the ECH group plants had significantly better ETR (electron transfer rate) and Fm (maximum fluorescence) and other key photosynthetic fluorescence indicators than the CKH group, indicating that the ECH group plants had better photosystem II structure integrity, higher photosynthetic electron transfer efficiency, and less damage from high temperature.
[0078] (6) The chlorophyll content of the EC group and the CK group before and after high temperature treatment is as follows: Figure 6 As shown in the figure, a represents the chlorophyll content in the leaf, and b represents the chlorophyll content in the petiole. It can be seen that before high-temperature treatment, there were no significant differences in the total chlorophyll (Chl T), chlorophyll a (Chl a), and chlorophyll b (Chl b) contents between the CK group and the EC group. After high-temperature stress, the contents of all three chlorophyll types in the ECH group were significantly higher than those in the CKH group, indicating that the treatment corresponding to the EC group can reduce chlorophyll degradation caused by high-temperature stress, maintain the material basis for plant photosynthesis, and thus improve the plant's tolerance to high-temperature stress.
[0079] (7) Figure 7The results of antioxidant enzyme activity, osmotic regulation capacity, and cell membrane damage tests before and after high-temperature treatment are shown in the EC and CK groups. In the EC group, a represents changes in proline content, b represents changes in malondialdehyde (MDA) content, c represents changes in CAT enzyme activity, d represents changes in POD enzyme activity, and e represents changes in SOD enzyme activity. It can be seen that compared with the control group (CK), the celery leaves of the CO2-enriched EC group showed significantly increased CAT, POD, and SOD antioxidant enzyme activities, decreased MDA content, and increased proline content. In the CKH group, which was treated with high temperature on top of the CK treatment, enzyme activity reached its peak, and MDA and proline accumulated significantly. In the ECH group, which was treated with high temperature on top of the EC treatment, enzyme activity was higher than CK and close to EC, MDA was lower than CKH, and proline showed no significant difference compared to EC. This indicates that CO2 enrichment treatment can enhance the antioxidant capacity of celery and alleviate the damage caused by high-temperature stress.
[0080] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims
1. A method for promoting celery growth and improving celery stress resistance through light regulation and CO2 treatment, characterized in that, Includes the following steps: When the celery grows to the "four leaves and one heart" stage, the photoperiod of the celery is regulated until the celery is harvested. The photoperiod is controlled to be 12h / 12h. During the rapid growth period of the celery, the celery is treated with CO2 enrichment for 25-30 days.
2. The method for promoting celery growth and improving celery stress resistance through light regulation and CO2 treatment as described in claim 1, characterized in that, The rapid growth period of celery is specifically 45-85 days after the seedling age.
3. The method for promoting celery growth and improving celery stress resistance through light regulation and CO2 treatment as described in claim 1, characterized in that, The CO2 concentration in the CO2 enrichment treatment is 800 ppm.
4. The method for promoting celery growth and improving celery stress resistance through light regulation and CO2 treatment as described in claim 1, characterized in that, The CO2 enrichment treatment is performed once a day, starting 1 hour after the daily photocycle and lasting for 4 hours.
5. The method for promoting celery growth and improving celery stress resistance through light regulation and CO2 treatment as described in claim 1, characterized in that, The illuminance during the photoperiod is 15400 lx.
6. The method for promoting celery growth and improving celery stress resistance by means of light regulation and CO2 treatment as described in claim 1, characterized in that, The celery in question is Ventura celery.
7. The method for promoting celery growth and improving celery stress resistance through light regulation and CO2 treatment as described in claim 1, characterized in that, The improvement of celery's stress resistance specifically refers to improving celery's resistance to high-temperature stress.