Exogenous regulation and control reagent for enhancing high-temperature stress tolerance of sea grass and slow release device

Abstract

The invention relates to the technical field of biology, in particular to an exogenous regulation and control reagent and a slow release device for enhancing high-temperature stress tolerance of sea grass, and the reagent comprises 0.5-2 parts of salicylic acid, 5-15 parts of trehalose, 1-5 parts of betaine and 0.05-0.3 part of ethylenediamine disuccinic acid. The slow-release device comprises a shell, a driving part is arranged in the shell, and a stirring assembly is arranged on an output shaft of the driving part; a driving rod is arranged below the driving part, an output shaft of the driving part is coaxially and fixedly connected with the driving rod, hinge rods are arranged at the two ends of the driving rod, supporting frames are symmetrically arranged in the shell, extension rods are arranged at the bottoms of the supporting frames, the hinge rods are hinged to the extension rods, a gas storage box is arranged on the shell, the gas storage box is filled with inert gas, and gas supply assemblies are arranged on the extension rods. According to the method, the activity of an antioxidant enzyme system of the aquatic plants is enhanced after salicylic acid is absorbed by the aquatic plants, air holes are kept open in cooperation with osmotic regulation of betaine, absorption of CO2 is promoted, the diffusion rate of CO2 is increased, and a sufficient substrate is provided for photosynthesis.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, specifically to an exogenous regulatory agent and a sustained-release device for enhancing the high-temperature stress tolerance of seaweed. Background Technology

[0002] Exogenous regulatory agents that enhance the heat stress tolerance of seagrass are chemical or biological agents that improve the heat resistance of seagrass by regulating its physiological metabolic processes. Through mechanisms such as activating the antioxidant defense system, optimizing photosynthetic performance, reprogramming metabolic pathways, and maintaining cell structural stability, they can alleviate problems such as seagrass membrane lipid peroxidation, decreased photosynthetic efficiency, and inhibited growth caused by high temperatures, thereby enhancing the survival ability of seagrass under high temperature stress and providing a scientific basis for marine ecological restoration and algal stress-resistant breeding.

[0003] Existing exogenous regulatory agents, such as salicylic acid, can be absorbed by seagrass leaves or roots after being applied to the growth area of ​​seagrass. They can also act as a stress warning signal to activate the systemic acquired resistance pathways of seagrass in advance. At the same time, they can enhance the activity of antioxidant enzyme systems, thereby clearing the excessive accumulation of reactive oxygen species under high temperature stress, reducing oxidative damage, protecting the functional integrity of photosynthetic organs, and maintaining photosynthetic efficiency.

[0004] While the aforementioned reagents can maintain photosynthetic efficiency, salicylic acid can induce stomatal closure in a concentration-dependent manner under high temperatures, potentially leading to insufficient CO2 supply and thus limiting the photosynthetic rate. Therefore, it is necessary to propose an exogenous regulatory agent and slow-release device that can maintain CO2 supply and enhance the high-temperature stress tolerance of seagrass. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides an exogenous regulatory reagent and a slow-release device for enhancing the high-temperature stress tolerance of seagrass. By adding salicylic acid and betaine to the exogenous regulatory reagent to create a synergistic effect, the salicylic acid is absorbed by the seagrass, enhancing the activity of the seagrass' antioxidant enzyme system. In conjunction with the osmotic regulation of betaine, stomatal opening is maintained, promoting CO2 absorption and increasing the CO2 diffusion rate, thus providing sufficient substrate for photosynthesis.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: an exogenous regulatory agent for enhancing the tolerance of seaweed to high temperature stress, comprising 0.5-2 parts of salicylic acid, 5-15 parts of trehalose, 1-5 parts of betaine and 0.05-0.3 parts of ethylenediaminedisuccinic acid.

[0007] Furthermore, an exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass comprises 1 part salicylic acid, 10 parts trehalose, 2 parts betaine and 0.1 part ethylenediamine disuccinic acid.

[0008] Furthermore, an exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass comprises 0.5 parts salicylic acid, 5 parts trehalose, 1 part betaine, and 0.25 parts ethylenediaminedisuccinic acid.

[0009] Furthermore, an exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass comprises 2 parts salicylic acid, 15 parts trehalose, 5 parts betaine, and 0.3 parts ethylenediamine disuccinic acid.

[0010] Furthermore, the method for preparing exogenous regulatory agents to enhance the high-temperature stress tolerance of seagrass includes the following steps:

[0011] Step 1, Preparation of mixed solution: Seawater is desalinated and sterilized to obtain a solvent. Salicylic acid, trehalose, betaine and ethylenediaminedisuccinic acid are added to the solvent as raw materials. The mixture is stirred by the stirring component in the slow-release device until it is completely dissolved to obtain a composite reagent. Then sodium alginate powder is added to the composite reagent and stirred until it is clear and viscous to obtain a mixed solution.

[0012] Step 2, preparation of gel spheres: The mixed solution is added dropwise to a 2%-5% calcium chloride solution using the sharp-hole-coagulation bath method, so that it undergoes a cross-linking reaction with calcium ions to form sodium alginate calcium gel spheres.

[0013] Step 3, Capsule preparation: Immerse sodium calcium alginate gel balls in a chitosan solution with pH < 6.3, so that chitosan molecules form a dense film on the surface of the sodium calcium alginate gel balls through electrostatic interaction, and encapsulate the sodium calcium alginate gel balls inside the dense film to form exogenous regulated capsules.

[0014] Step 4, Washing and Storage: Remove the exogenous regulation capsule and wash it with deionized water. The washing process is completed when the washing solution is neutral, thus obtaining the exogenous regulation reagent.

[0015] Furthermore, in step one, when adding sodium alginate powder, it is added in batches continuously, and the amount added decreases as the viscosity of the composite reagent increases.

[0016] Furthermore, in step two, the diameter of the gel spheres is 2-5 mm.

[0017] Furthermore, a slow-release device for enhancing the high-temperature stress tolerance of seaweed includes a controller and a housing. The housing has an observation window on its side wall, an inlet on the upper part of the side wall with a hinged opening and closing block, and an outlet on the lower part of the side wall with a detachable rubber plug. A drive unit is fixedly connected to the top inner wall of the housing. The controller controls the opening and closing of the drive unit, and the output shaft of the drive unit is equipped with a stirring component for stirring the material inside the housing.

[0018] A drive rod is located below the drive unit. The output shaft of the drive unit is coaxially and fixedly connected to the drive rod. Both ends of the drive rod are hinged with hinge rods. Support frames are symmetrically and fixedly connected to the top wall of the housing. Extension rods are laterally slidably fitted at the bottom of each support frame. Each hinge rod is hinged to one end of its adjacent extension rod. A gas storage box is fixedly connected to the top of the housing. The gas storage box is filled with inert gas. Each extension rod is equipped with a gas supply component for supplying inert gas into the housing at the end away from the hinge rod.

[0019] Furthermore, the stirring assembly includes a stirring rod coaxially fixedly connected to the output shaft of the drive component, with one end of the stirring rod away from the output shaft of the drive component rotatably engaged with the bottom wall of the housing, and stirring blades fixedly connected to the stirring rod circumferentially along its side wall.

[0020] Furthermore, the air supply assembly includes an air supply box fixedly connected to the inner wall of the outer shell. An air supply plate is laterally slidably fitted onto the inner wall of the air supply box. The end of the extension rod away from the hinge rod extends through the side wall of the air supply box and into the air supply box, where it is fixedly connected to the air supply plate. An air inlet cylinder and an air outlet cylinder are connected to the air supply box, and an air inlet check valve and an air outlet check valve are respectively connected at their connection points. The end of the air inlet cylinder away from the air supply box is connected to the interior of the air storage tank, and the end of the air outlet cylinder away from the air supply box is fixedly connected to the bottom wall of the outer shell. Several air outlet holes are opened on the air outlet cylinder, and the air outlet cylinder is connected to the interior of the outer shell through the air outlet holes.

[0021] The technical principles of the above solution are as follows:

[0022] Workers added salicylic acid, trehalose, betaine, and ethylenediaminedisuccinic acid (EDDISA) to a solvent. The solvent was then injected into the casing through the inlet, and the output shaft of the drive unit was controlled by a controller to rotate reciprocally. At this time, the output shaft of the drive unit drove the stirring assembly to operate, mixing the salicylic acid, trehalose, betaine, EDDISA, and solvent.

[0023] During this process, the output shaft of the drive unit also drives the drive rod to rotate back and forth, which in turn drives the hinge rod to swing back and forth. The hinge rod drives the extension rod to move back and forth, which in turn drives the gas delivery component to operate, delivering inert gas to the solvent. The flow of gas forms turbulence, which improves the uniformity of mixing and reduces the oxidation of various components in the solvent.

[0024] The above approach has the following beneficial effects:

[0025] 1. This invention adds salicylic acid and betaine to an exogenous regulatory reagent to create a synergistic effect. After the salicylic acid is absorbed by aquatic plants, it enhances the activity of the antioxidant enzyme system of aquatic plants. In conjunction with the osmotic regulation of betaine, it maintains stomatal opening, promotes CO2 absorption, and increases the CO2 diffusion rate, thus providing sufficient substrate for photosynthesis.

[0026] 2. The sodium alginate calcium gel spheres in this invention can exist stably in the seawater environment. When the pH value of seaweed decreases due to high temperature stress, the electrostatic repulsion of the chitosan membrane weakens and the membrane pore size increases, which can trigger the slow release of active ingredients such as salicylic acid and trehalose. This ensures that the active ingredients can be released in a concentrated manner when the high temperature stress is most severe, thereby improving the resource utilization efficiency.

[0027] 3. By injecting inert gas into the shell, the present invention reduces the probability of oxidation of the materials inside the shell when they are stirred and mixed, thereby reducing the degradation rate of easily oxidized components such as salicylic acid and inhibiting the growth of microorganisms.

[0028] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0029] Figure 1 This is a schematic diagram illustrating the preparation steps of an example of the exogenous regulatory reagent for enhancing the high-temperature stress tolerance of seaweed according to the present invention;

[0030] Figure 2 This is an isometric schematic diagram of an embodiment of the sustained-release device for enhancing the high-temperature stress tolerance of seaweed according to the present invention;

[0031] Figure 3 This is an isometric schematic diagram of the internal structure of an embodiment of the sustained-release device for enhancing the high-temperature stress tolerance of seaweed according to the present invention;

[0032] Figure 4 This is a cross-sectional view of the air delivery component in an embodiment of the slow-release device for enhancing the high-temperature stress tolerance of seaweed according to the present invention.

[0033] The reference numerals in the accompanying drawings of the instruction manual include: 1. outer casing; 2. opening and closing block; 3. rubber plug; 4. servo motor; 5. stirring rod; 6. stirring blade; 7. drive rod; 8. hinge rod; 9. support frame; 10. extension rod; 11. air storage tank; 12. air supply tank; 13. air supply plate; 14. air inlet cylinder; 15. air outlet cylinder; 16. air inlet check valve; 17. air outlet check valve. Detailed Implementation

[0034] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0036] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0037] The following detailed description illustrates the specific implementation method:

[0038] Example 1:

[0039] An exogenous regulatory agent for enhancing the high-temperature stress tolerance of seaweed comprises 1 part salicylic acid, 10 parts trehalose, 2 parts betaine and 0.1 part ethylenediaminedisuccinic acid.

[0040] like Figure 1 As shown, its manufacturing method includes the following steps:

[0041] Step 1, Preparation of Mixed Solution: The seawater is first desalinated and sterilized, and then used as a solvent. In this embodiment, the seawater is desalinated and sterilized by distillation, and the solvent quantity is 1000 parts.

[0042] After the solvent was prepared, the staff added salicylic acid, trehalose, betaine and ethylenediaminedisuccinic acid as raw materials into the solvent. Then the solvent was injected into the slow-release device and stirred by the stirring component in the slow-release device. After all the solid components in the solvent were completely dissolved, the composite reagent was obtained.

[0043] Subsequently, the staff added sodium alginate powder to the composite reagent and stirred it until it became clear and viscous, obtaining a mixed solution. The sodium alginate powder was added in batches continuously, with the amount added decreasing as the viscosity of the composite reagent increased.

[0044] Step 2, Gel sphere preparation: After preparing the mixed solution, the operator adds the mixed solution dropwise to a 3% calcium chloride solution using the sharp-hole-coagulation bath method, causing it to cross-link with calcium ions to form sodium alginate calcium gel spheres with a diameter of 2-5 mm. In this embodiment, the sharp-hole-coagulation bath method is existing technology and will not be described in detail.

[0045] Step 3, Capsule Preparation: The staff immersed sodium calcium alginate gel balls in a chitosan solution with a pH < 6.3, so that the chitosan molecules formed a dense film on the surface of the sodium calcium alginate gel balls through electrostatic interaction, and the sodium calcium alginate gel balls were wrapped inside the dense film to form exogenous regulatory capsules.

[0046] Step 4, Washing and Storage: The staff takes out the exogenous regulation capsules, washes them with deionized water, and measures the acidity or alkalinity of the washing solution. Washing is stopped when the washing solution is neutral. At this point, the exogenous regulation capsules are the prepared exogenous regulation reagents.

[0047] like Figure 2 As shown, the slow-release device includes a controller and a housing 1. The housing 1 has an observation window on its side wall, a feed inlet on the upper part of its side wall, and an opening / closing block 2 hinged to the feed inlet. The housing 1 also has a discharge outlet on its lower side wall, with a detachable rubber plug 3 attached to the discharge outlet. A drive unit is screwed to the inner top wall of the housing 1. The controller controls the opening and closing of the drive unit. A stirring assembly for stirring the material inside the housing 1 is mounted on the output shaft of the drive unit. In this embodiment, a servo motor 4 is used as the drive unit.

[0048] like Figure 3 As shown, the stirring assembly includes a stirring rod 5 that is coaxially screwed and fixed to the output shaft of the drive component. The end of the stirring rod 5 away from the output shaft of the drive component is rotatably engaged with the bottom wall of the inner shell 1. The stirring rod 5 has stirring blades 6 integrally formed along its side wall circumferentially.

[0049] Specifically, the staff injects a solvent containing salicylic acid, trehalose, betaine, and ethylenediaminedisuccinic acid into the slow-release device through the inlet. The controller then controls the output shaft of the servo motor 4 to reciprocate, which in turn drives the stirring rod 5, which is coaxially screwed to it, to reciprocate. The stirring rod 5, in turn, drives the integrally formed stirring blade 6 to reciprocate, thus stirring the solvent and dissolving its components. During this process, the staff can observe the dissolution of each substance in the solvent in real time through an observation window.

[0050] like Figure 3 and Figure 4As shown, a drive rod 7 is provided below the drive component. The output shaft of the drive component is coaxially screwed and fixedly connected to the drive rod 7. Both ends of the drive rod 7 are hinged with hinge rods 8. Support frames 9 are symmetrically welded on the inner top wall of the outer shell 1. Extension rods 10 are laterally slidably fitted at the bottom of each support frame 9. The hinge rods 8 are all hinged to one end of their adjacent extension rods 10. An air storage box 11 is integrally formed on the top of the outer shell 1. The air storage box 11 is filled with inert gas. The end of the extension rod 10 away from the hinge rod 8 is provided with a gas supply component for supplying inert gas into the outer shell 1.

[0051] like Figure 4 As shown, the air supply assembly includes an air supply box 12 welded to the inner wall of the outer shell 1. An air supply plate 13 is laterally slidably fitted on the inner wall of the air supply box 12. The end of the extension rod 10 away from the hinge rod 8 extends through the side wall of the air supply box 12 and into the air supply box 12, forming an integral part with the air supply plate 13. An air inlet cylinder 14 and an air outlet cylinder 15 are connected to the air supply box 12. An air inlet check valve 16 and an air outlet check valve 17 are respectively connected at their connection points. The end of the air inlet cylinder 14 away from the air supply box 12 is connected to the interior of the air storage tank 11. The end of the air outlet cylinder 15 away from the air supply box 12 is welded to the inner bottom wall of the outer shell 1. Several air outlet holes are opened on the air outlet cylinder 15, and the air outlet cylinder 15 is connected to the interior of the outer shell 1 through the air outlet holes. In this embodiment, the flow direction of the intake check valve 16 is one-way from the intake cylinder 14 to the inside of the air supply box 12, and the flow direction of the outlet check valve 17 is one-way from the inside of the air supply box 12 to the outlet cylinder 15.

[0052] Specifically, during the rotation of the output shaft of the servo motor 4, it also drives the drive rod 7, which is fixedly connected to it by screws, to reciprocate. At this time, since the two ends of the hinge rod 8 are respectively hinged to the drive rod 7 and the adjacent extension rod 10, and the extension rod 10 is in sliding engagement with the adjacent support frame 9, when the drive rod 7 rotates, it can drive the hinge rod 8, which is hinged to it, to swing, and then the hinge rod 8 drives the extension rod 10, which is hinged to it, to move laterally back and forth.

[0053] During this process, the extension rod 10 also drives the integrally formed gas delivery plate 13 to slide back and forth within the gas delivery box 12, drawing the inert gas located in the gas storage box 11 into the gas delivery box 12 through the air inlet cylinder 14 and the air inlet one-way valve 16. Then, the inert gas in the gas delivery box 12 is discharged into the air outlet cylinder 15 through the air outlet one-way valve 17, allowing it to enter the interior of the outer shell 1 through the air outlet hole located on the air outlet cylinder 15. The flow of gas creates turbulence, thereby improving the stirring efficiency of the solvent. At the same time, after entering the solvent, the inert gas can also protect the easily oxidized components in the solvent, thereby reducing the possibility of oxidation of the easily oxidized components in the solvent.

[0054] This invention achieves a synergistic effect by adding salicylic acid and betaine to an exogenous regulatory reagent. After being absorbed by aquatic plants, salicylic acid enhances the activity of the antioxidant enzyme system of aquatic plants, and betaine, in conjunction with its osmotic regulation, maintains stomatal opening, promotes CO2 absorption, and increases the CO2 diffusion rate, thus providing sufficient substrate for photosynthesis.

[0055] Example 2:

[0056] As attached Figure 1 As shown, the difference from Example 1 is that an exogenous regulatory agent for enhancing the high temperature stress tolerance of seaweed includes 0.5 parts salicylic acid, 5 parts trehalose, 1 part betaine and 0.25 parts ethylenediamine disuccinic acid.

[0057] Its preparation method is the same as that in Example 1.

[0058] Example 3:

[0059] As attached Figure 1 As shown, the difference from Example 2 is that an exogenous regulatory agent for enhancing the high temperature stress tolerance of seaweed includes 2 parts salicylic acid, 15 parts trehalose, 5 parts betaine and 0.3 parts ethylenediamine disuccinic acid.

[0060] Its preparation method is the same as that in Example 1.

[0061] The exogenous regulatory reagent from Example 1 was used as experimental group 1, the exogenous regulatory reagent from Example 2 as experimental group 2, and the exogenous regulatory reagent from Example 3 as experimental group 3. An exogenous regulatory reagent using only salicylic acid was used as the control group for comparative experiments. The results are shown in the table below:

[0062] Table 1. Control Experiment Table

[0063] As shown in the table, the malondialdehyde (MDA) content in experimental groups 1, 2, and 3 was lower than that in the control group, indicating that the seaweed cells experienced less oxidative damage after using the regulatory reagents in these groups. Simultaneously, the photosynthetic efficiency and chlorophyll content in experimental groups 1, 2, and 3 were higher than those in the control group, indicating that the photosynthetic function of seaweed was superior to that of the control group. In conclusion, experimental groups 1, 2, and 3 were all superior to the control group, with experimental group 1 being the optimal choice.

[0064] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. An exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass, characterized in that, It includes 0.5-2 parts salicylic acid, 5-15 parts trehalose, 1-5 parts betaine and 0.05-0.3 parts ethylenediamine disuccinic acid.

2. The exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass according to claim 1, characterized in that, It includes 1 part salicylic acid, 10 parts trehalose, 2 parts betaine and 0.1 part ethylenediamine disuccinic acid.

3. The exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass according to claim 2, characterized in that, It includes 0.5 parts salicylic acid, 5 parts trehalose, 1 part betaine and 0.25 parts ethylenediamine disuccinic acid.

4. The exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass according to claim 3, characterized in that, It includes 2 parts salicylic acid, 15 parts trehalose, 5 parts betaine and 0.3 parts ethylenediamine disuccinic acid.

5. The exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass according to claim 4, characterized in that, Its production method includes the following steps: Step 1, Preparation of mixed solution: Seawater is desalinated and sterilized to obtain a solvent. Salicylic acid, trehalose, betaine and ethylenediaminedisuccinic acid are added to the solvent as raw materials. The mixture is stirred by the stirring component in the slow-release device until it is completely dissolved to obtain a composite reagent. Then sodium alginate powder is added to the composite reagent and stirred until it is clear and viscous to obtain a mixed solution. Step 2, preparation of gel spheres: The mixed solution is added dropwise to a 2%-5% calcium chloride solution using the sharp-hole-coagulation bath method, so that it undergoes a cross-linking reaction with calcium ions to form sodium alginate calcium gel spheres; Step 3, Capsule preparation: Sodium calcium alginate gel balls are immersed in a chitosan solution with pH < 6.3, so that chitosan molecules form a dense film on the surface of sodium calcium alginate gel balls through electrostatic interaction, and the sodium calcium alginate gel balls are wrapped inside the dense film to form exogenous regulated capsules. Step 4, Washing and Storage: Remove the exogenous regulation capsule and wash it with deionized water. The washing process is completed when the washing solution is neutral, thus obtaining the exogenous regulation reagent.

6. The exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass according to claim 5, characterized in that, In step one, when adding sodium alginate powder, it is added in batches continuously, and the amount added decreases as the viscosity of the composite reagent increases.

7. The exogenous regulatory agent for enhancing the high-temperature stress tolerance of seagrass according to claim 6, characterized in that, In step two, the diameter of the gel spheres is 2-5 mm.

8. A sustained-release device for enhancing the high-temperature stress tolerance of seagrass, applicable to any one of the exogenous regulatory agents for enhancing the high-temperature stress tolerance of seagrass according to claims 1-7, characterized in that, Includes a controller and a housing (1). The housing (1) has an observation window on its side wall, an inlet on the upper part of the side wall, a hinged opening block (2) at the inlet, and an outlet on the lower part of the side wall, with a detachable rubber plug (3) at the outlet. A drive unit is fixedly connected to the inner top wall of the housing (1). The controller is used to control the opening and closing of the drive unit. A stirring assembly for stirring the material inside the housing (1) is provided on the output shaft of the drive unit. A drive rod (7) is provided below the drive component. The output shaft of the drive component is coaxially fixedly connected to the drive rod (7). Both ends of the drive rod (7) are hinged with hinge rods (8). Support frames (9) are symmetrically fixedly connected to the top wall of the outer shell (1). Extension rods (10) are laterally slidably fitted at the bottom of the support frames (9). The hinge rods (8) are all hinged to one end of their adjacent extension rods (10). A gas storage box (11) is fixedly connected to the top of the outer shell (1). The gas storage box (11) is filled with inert gas. The end of the extension rod (10) away from the hinge rods (8) is provided with a gas supply component for supplying inert gas into the outer shell (1).

9. The sustained-release device for enhancing the high-temperature stress tolerance of seaweed according to claim 8, characterized in that, The stirring assembly includes a stirring rod (5) coaxially fixedly connected to the output shaft of the drive unit. The end of the stirring rod (5) away from the output shaft of the drive unit is rotatably engaged with the bottom wall of the outer casing (1). The stirring rod (5) is circumferentially fixedly connected with stirring blades (6) along its side wall.

10. The sustained-release device for enhancing the high-temperature stress tolerance of seaweed according to claim 9, characterized in that, The air supply assembly includes an air supply box (12) fixedly connected to the inner wall of the outer shell (1). An air supply plate (13) is slidably fitted to the inner wall of the air supply box (12). One end of the extension rod (10) away from the hinge rod (8) extends through the side wall of the air supply box (12) and into the air supply box (12) and is fixedly connected to the air supply plate (13). An air inlet cylinder (14) and an air outlet cylinder (15) are connected to the air supply box (12). An air inlet check valve (16) and an air outlet check valve (17) are connected to the air outlet cylinder (14). One end of the air inlet cylinder (14) away from the air supply box (12) is connected to the inside of the air storage tank (11). One end of the air outlet cylinder (15) away from the air supply box (12) is fixedly connected to the bottom wall of the outer shell (1). Several air outlet holes are opened on the air outlet cylinder (15). The air outlet cylinder (15) is connected to the inside of the outer shell (1) through the air outlet holes.

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