Quantitative-change-controllable intermittent inoculation track control method for liquid strains

Abstract

The invention discloses a controllable quantitative change intermittent inoculation track control method for a liquid strain. The method comprises the following steps: in a liquid strain supplementing state, introducing the liquid strain in a liquid storage bin into a liquid storage pipe; in the liquid strain inoculation state, the liquid strains in the liquid storage pipe are introduced into all the track control valves, the corresponding track control valves are opened according to inoculation track setting, and the liquid strains continue to be supplemented into the liquid storage pipe through the liquid storage bin; in the inoculation state of the liquid strain, when the liquid strain is introduced into the liquid storage pipe, the track control valve can complete intermittent work, the pressure of the liquid storage pipe can be adjusted according to a spacing set value, and the track spacing distance of the liquid strain is set according to the actual parameter requirement of the strain bag breeding time. The tracks of the liquid strains at least comprise equidistant arrangement in a shape like a Chinese character'pin ', parallel intermittent arrangement and asymmetric arrangement. According to the method, the inoculation amount and the inoculation track of the liquid strain can be controlled, too large inoculation amount is avoided, the comprehensive input cost of a fungus bag is effectively reduced, and the quality standard of the fungus bag is increased.

Description

Technical Field

[0001] This invention relates to the field of inoculation trajectory control technology for liquid spawn bulk technology of edible fungi spawn bags (the substrate is sterilized before bagging, and inoculation is carried out simultaneously during bagging), and in particular to a method for intermittent inoculation trajectory control with controllable quantitative changes in liquid spawn. Background Technology

[0002] There are currently two methods for inoculating traditional edible mushroom spawn bags with liquid spawn: 1. Single-sided inoculation: Traditional edible mushroom bags use a production process of first packing the substrate, then sterilizing, cooling, and inoculating. Regardless of whether it is solid or liquid spawn, the spawn can only be inoculated on the surface or top of the material (such as the ring-type bag making method).

[0003] Disadvantages: The inoculation point is singular, and can only be inoculated on the surface of the substrate inside the bag or in the pores of the substrate. There are few contact points for the spawn, and the mycelium is in a unidirectional growth trajectory, which leads to a long inoculation period, inconsistent mycelial age inside the substrate, and a high contamination rate. The inoculation period of the spawn bag is generally 25-40 days for most varieties, while for some rare varieties such as red-topped bamboo fungus, the inoculation period can be as long as 90-120 days for the mycelium to fully colonize the bag.

[0004] 2. Bulk material double-sided inoculation: The existing bulk material briquette manufacturing process adopts the production process of first sterilizing the substrate in a fully enclosed sterilization chamber, then cooling it in a closed cooling chamber, and finally packaging and inoculating simultaneously. The existing bulk material process currently uses liquid inoculum inoculation. Taking shiitake mushroom briquette as an example, the inoculation trajectory can only be in the center of the substrate inside the briquette bag and the surface of the substrate.

[0005] Advantages: The inoculation surface has one more surface than single-sided inoculation, and the mycelium is in a bidirectional growth state, which can greatly shorten the cultivation cycle of the spawn bag. It has low contamination. Generally, the mycelium cultivation of spawn bags of general varieties only takes 7-12 days to fill the bag using the bulk material sterilization production process.

[0006] The two inoculation methods mentioned above share a common characteristic: the liquid inoculation process of a single spawn bag is completed in one go. In particular, during the inoculation of spawn bags made from existing bulk materials, the output liquid inoculum leaves complete and unbroken inoculation tracks in the center and outer surface of the substrate inside the spawn bag. After the inoculation program is set, the inoculation amount of each spawn bag is basically consistent and fixed. It lacks the ability to control the inoculation program without variables, inoculation track deformation, and equivalent variables. Summary of the Invention

[0007] The purpose of this invention is to provide a method for controlling the intermittent inoculation trajectory of liquid spawn with controllable quantitative changes, so as to solve the problems existing in the prior art, make the inoculation amount and inoculation trajectory morphology of liquid spawn controllable, avoid excessive inoculation amount, effectively reduce the overall input cost of spawn bags and increase the quality standards of spawn bags.

[0008] To achieve the above objectives, the present invention provides the following solution: This invention provides a method for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative changes. The method utilizes a device for controlling the intermittent inoculation trajectory of liquid bacterial strains, including a liquid bacterial strain replenishment state and a liquid bacterial strain inoculation state. The liquid culture replenishment process involves introducing the liquid culture from the storage tank into the storage pipe. The liquid inoculation state is as follows: the liquid inoculation in the storage tube is introduced into each trajectory control valve, and the corresponding trajectory control valve is opened according to the inoculation trajectory setting. At the same time, the liquid inoculation continues to be replenished into the storage tube through the storage tank. When liquid culture is introduced into the storage tube during liquid culture inoculation, the trajectory control valve can operate intermittently, and the pressure of the storage tube can be adjusted according to the spacing setting value. The trajectory interval of the liquid culture can be set according to the actual parameters of the culture bag incubation time. The trajectory of the liquid culture includes at least three-dimensional equidistant arrangement, parallel intermittent arrangement, and asymmetrical arrangement.

[0009] Preferably, the liquid culture replenishment state is the initial state, and the liquid culture inoculation state includes an extended state and a retracted state, wherein, The extended state is as follows: the liquid inoculum in the storage tube is introduced into each trajectory control valve through the first route, the corresponding trajectory control valve is opened according to the inoculation trajectory setting, and the liquid inoculum continues to be replenished into the storage tube through the storage chamber; The retracted state is as follows: the liquid inoculum in the storage tube is introduced into each trajectory control valve through the second route, the corresponding trajectory control valve is opened according to the inoculation trajectory setting, and the liquid inoculum continues to be replenished into the storage tube through the storage chamber. In both the extended and retracted states, when the intermittent inoculation trajectory control device for controllable quantitative change of liquid microorganisms includes a drive structure, and both the inoculation structure and the drive structure are a set, the drive structure is controlled to complete the intermittent working state, and / or each trajectory control valve is controlled to complete the intermittent working state. When both the inoculation structure and the drive structure are two sets, the drive structure is controlled to complete the alternating working state, and / or each trajectory control valve is controlled to complete the intermittent working state.

[0010] Preferably, in the initial state, the method for introducing the liquid bacteria in the storage tank into the storage pipe is as follows: the liquid bacteria are introduced into the storage tank through the inlet pipe, the second valve is opened, and at the same time the drive structure drags the liquid control plate to move towards the second fixed plate and return to the zero position. The liquid bacteria pass through the storage tank, the inlet pipe and the second valve in sequence to the storage pipe.

[0011] Preferably, when in the extended state, the method for ensuring that the liquid inoculum reaches each trajectory control valve via the first route is as follows: close the second valve, open the first valve, drive the structure to move the liquid control plate toward the second fixed plate, and simultaneously open the fourth valve. The liquid inoculum then passes through the storage tube sequentially via the fourth valve, the second outlet tube, the third outlet tube, and the fifth valve to reach each trajectory control valve. The corresponding trajectory control valve is then opened according to the inoculation trajectory setting.

[0012] Preferably, when in the extended state, the method for replenishing the liquid bacterial culture into the storage tube via the storage tank is as follows: the liquid bacterial culture is sequentially delivered from the storage tank through the supply tube, the first valve, and the first delivery tube into the storage tube, thereby replenishing the liquid bacterial culture into the storage tube.

[0013] Preferably, when in the retracted state, the method for ensuring that the liquid inoculum reaches each trajectory control valve via the second route is as follows: close the first valve, open the second valve, drive the liquid control plate to move towards the first fixed plate, and simultaneously open the third valve. The liquid inoculum then passes through the storage tube sequentially through the first outlet tube, the third valve, the second outlet tube, the third outlet tube, and the fifth valve to reach each trajectory control valve. The corresponding trajectory control valve is then opened according to the inoculation trajectory setting.

[0014] Preferably, when in the retracted state, the method for replenishing the liquid bacterial culture to the storage tube via the storage tank is as follows: the liquid bacterial culture is sequentially delivered from the storage tank to the storage tube via the supply tube, the second valve, and the second delivery tube, thereby replenishing the liquid bacterial culture to the storage tube.

[0015] Preferably, it also includes an auxiliary control state, which is as follows: By using shut-off valves or overflow valves to limit the pressure values ​​at various points of the intermittent inoculation trajectory control device that controls the amount of liquid bacteria, the inoculation trajectory can be set and the safety of the components can be protected.

[0016] The present invention achieves the following technical effects compared to the prior art: This invention provides a method for controlling the intermittent inoculation trajectory of liquid microorganisms. In the liquid microorganism replenishment state, liquid microorganisms from the storage tank are introduced into the storage tube. In the liquid microorganism inoculation state, liquid microorganisms from the storage tube are introduced into each trajectory control valve, and the corresponding trajectory control valves are opened according to the inoculation trajectory settings. Simultaneously, liquid microorganisms continue to be replenished into the storage tube via the storage tank. During the liquid microorganism inoculation state, when liquid microorganisms are introduced into the storage tube, the pressure in the storage tube is intermittent and adjustable according to the interval setting. Therefore, inoculation within the microbial bag can be achieved without a drive structure; that is, liquid microorganisms are injected into the storage tube, and then the storage tube is pressurized. When intermittent injection of liquid microorganisms into the microbial bag is required, only the opening and closing frequency of each trajectory control valve needs to be controlled to achieve intermittent operation of the trajectory control valves. Simultaneously, when continuously supplying bacteria into the spawn bag, maintaining pressure balance within the storage tube is sufficient, simplifying the operation. During this process, the interval distance of the liquid spawn trajectory, the density of the inoculation trajectory, and the density of the inoculation dimension are set according to the actual parameters of the spawn bag's growth time, thereby determining the spawn bag's mycelium growth cycle. The liquid spawn trajectory includes at least a triangular equidistant arrangement, a parallel discontinuous arrangement, and an asymmetrical arrangement. This directly changes the continuous, uninterrupted liquid inoculation trajectory within the spawn bag of the existing bulk inoculation device, forming a novel, controllable quantitative change and discontinuous special inoculation trajectory of the liquid spawn within the edible mushroom spawn bag. This effectively avoids excessive inoculation volume, reduces input costs, and significantly increases the number of robust mycelial colonies within the spawn bag (more germination points and more weak mycelia). Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strain in Example 1 at one angle (the bag surface inoculation structure is omitted). Figure 2 This is a schematic diagram of the intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strain in Example 1 from another angle (the bag surface inoculation structure is omitted). Figure 3 for Figure 1 Top view; Figure 4 for Figure 1 The right view; Figure 5 This is a schematic diagram of the bag surface inoculation structure in Example 1; Figure 6 for Figure 5 The main view; Figure 7 for Figure 6 AA section view; Figure 8 for Figure 5 Side view; Figure 9 To establish a continuous inoculation trajectory for shiitake mushroom spawn using existing bulk spawn production technology. Figure 1 ; Figure 10 To generate a parallel discontinuous trajectory diagram for shiitake mushroom logs using the intermittent inoculation trajectory control method with controllable quantitative change of liquid inoculum in Example 2; Figure 11 To establish a continuous inoculation trajectory for black fungus spawn bags using existing bulk spawn production technology. Figure 1 ; Figure 12 To generate a parallel discontinuous trajectory diagram for the black fungus spawn using the intermittent inoculation trajectory control method with controllable quantitative change of liquid spawn in Example 2; Figure 13 To implement continuous inoculation of shiitake mushroom spawn using existing bulk spawn production technology. Figure 2 ; Figure 14 To create a triangular intermittent trajectory diagram for shiitake mushroom logs using the intermittent inoculation trajectory control method with controllable quantitative change of liquid inoculum in Example 2; Figure 15 To establish a continuous inoculation trajectory for black fungus spawn bags using existing bulk spawn production technology. Figure 2 ; Figure 16 To generate a triangular intermittent trajectory diagram for the black fungus spawn bags using the intermittent inoculation trajectory control method with controllable quantitative change of liquid strains in Example 2; In the diagram: 1-Controllable intermittent inoculation trajectory control device for liquid inoculum, 2-Inoculation structure, 3-Bag surface inoculation structure, 4-Inoculum supply tube, 5-Storage tube, 6-First inoculum delivery tube, 7-Second inoculum delivery tube, 8-First valve, 9-Second valve, 10-First outlet tube, 11-Second outlet tube, 12-Third outlet tube, 13-Spare outlet tube, 14-Third valve, 15-Fourth valve, 16-Fifth valve, 17-Sixth valve, 18-First pneumatic valve body. 9-First valve body actuator, 20-Second pneumatic valve body, 21-Second valve body actuator, 22-Servo motor, 23-Transmission screw, 24-Liquid control board, 25-First fixed plate, 26-Second fixed plate, 27-Fixing rod, 28-Inoculum conveying shaft, 29-Material cylinder, 30-Spiral blade, 31-Control unit, 32-Bag surface inlet tube, 33-Trajectory control valve, 34-Flange, 35-Inoculum conveying channel, 36-Storage tank, 37-Inlet tube, 38-Main valve. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] The purpose of this invention is to provide a method for controlling the intermittent inoculation trajectory of liquid spawn with controllable quantitative changes, so as to solve the problems existing in the prior art, make the inoculation amount and inoculation trajectory of liquid spawn controllable, avoid excessive inoculation amount, effectively reduce the overall input cost of spawn bags and increase the quality standards of spawn bags.

[0021] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0022] Example 1 like Figures 1-8As shown, this embodiment provides a discontinuous inoculation trajectory control device 1 for controllable quantitative change of liquid microbial culture, including an inoculation structure 2, a bag surface inoculation structure 3, and at least one injection structure. When there is more than one injection structure, multiple injection structures are arranged in parallel. The inlet of the inoculation structure 2 is used for the introduction of liquid microbial culture, and the outlet of the inoculation structure 2 can be connected to each injection structure respectively. The injection structure includes a supply pipe 4, a storage pipe 5, a delivery pipe assembly, and an outlet pipe assembly. The supply pipe 4 is used for the introduction of liquid microbial culture, and a main valve 38 is provided on the supply pipe 4. The delivery pipe assembly is connected to the supply pipe 4 and the storage pipe 5 respectively. The inoculation tube assembly is connected to both ends of the inoculator, the bag surface inoculation structure 3, and the storage tube 5. The pressure of the storage tube 5 is intermittent and can be adjusted according to the interval setting value. This allows inoculation within the culture bag to be achieved without a drive structure; that is, liquid inoculum is injected into the storage tube 5, and then pressure is applied to the storage tube 5. When intermittent injection of liquid inoculum into the culture bag is required, only the opening and closing frequency of each control unit 31 needs to be controlled. When continuously inoculating into the culture bag, the pressure balance within the storage tube 5 needs to be maintained, making the operation simpler. When there is only one inoculation structure, the injection... The six rows of tubes in the inoculum structure can operate in a direct-injection intermittent manner. When there is more than one inoculum injection structure, the left and right inoculum injection structures can be interchanged. This ensures that the pressure value inside the liquid storage tube 5 remains relatively stable during continuous high-speed inoculum injection. Liquid inoculum is delivered to the inoculator and the bag surface inoculation structure 3 through the inoculum outlet tube assembly. The bag surface inoculation structure 3 includes multiple parallel control units 31. These control units 31 can be individually opened and closed according to the inoculation parameters (such as inoculation pressure, inoculation volume, and inoculation time) and the inoculation trajectory pattern, and are used to control the inoculation trajectory of the inner and outer layers of the substrate bag inside the inoculum bag. By changing the inoculation propulsion state of the drive structure from the original continuous spraying stroke to a controllable intermittent propulsion inoculation state, and cooperating with the intermittent working state of multiple control units 31, the continuous and uninterrupted liquid inoculation trajectory in the mushroom bag after inoculation of the existing bulk material inoculation device is directly changed, forming a new intermittent special inoculation trajectory with controllable quantitative change and inoculation trajectory change in the liquid inoculation in the edible mushroom bag. This effectively avoids excessive inoculation, reduces input costs, and significantly increases the number of robust mycelial colonies in the mushroom bag (more germination points and more weak mycelia).

[0023] The inoculation tube assembly includes a first inoculation tube 6 and a second inoculation tube 7. A first valve 8 is provided on the first inoculation tube 6, and a second valve 9 is provided on the second inoculation tube 7. One end of both the first and second inoculation tubes is connected to the inoculation tube 4. The other end of the first inoculation tube 6 is connected to one end of the storage tube 5, and the other end of the second inoculation tube 7 is connected to the other end of the storage tube 5. In this embodiment, while the liquid bacteria in the cavity on one side of the actuating element in the storage tube 5 are discharged into the inoculator for inoculation, the liquid bacteria in the storage chamber 36 enter the cavity on the other side of the actuating element in the storage tube 5, thereby replenishing the liquid bacteria.

[0024] The colony discharge tube assembly includes a first colony discharge tube 10, a second colony discharge tube 11, and a third colony discharge tube 12; or, the colony discharge tube assembly includes a first colony discharge tube 10, a second colony discharge tube 11, a third colony discharge tube 12, and at least one spare colony discharge tube 13. A third valve 14 is provided on the first colony discharge tube 10, a fourth valve 15 is provided on the second colony discharge tube 11, a fifth valve 16 is provided on the third colony discharge tube 12, and a sixth valve 17 is provided on the spare colony discharge tube 13. Both the fifth valve 16 and the sixth valve 17 are manual ball valves. The valve has the following configuration: one end of the first inoculum outlet tube 10 is connected to one end of the storage tube 5; one end of the second inoculum outlet tube 11 is connected to the other end of the storage tube 5; the other ends of both the first and second inoculum outlet tubes 10 and 11 are connected to one end of the third inoculum outlet tube 12; the other ends of both the first and second inoculum outlet tubes 10 and 11 are connected to one end of the spare inoculum outlet tube 13; and the other ends of the third inoculum outlet tube 12 and each spare inoculum outlet tube 13 are connected to different bag surface inoculation structures 3. In this embodiment, when a certain inoculum inoculation structure malfunctions, the spare inoculum outlet tube 13 can connect the normal inoculum inoculation structure to the inoculator, ensuring the inoculation efficiency of the inoculator and preventing the inoculation work of the inoculator and the inoculum bag surface from being affected by the malfunction of one inoculum inoculation structure.

[0025] In this embodiment, at least one driving structure is also included. The driving structure is used to drive the movement of the actuating element inside the liquid storage tube 5. The driving structure operates in an intermittent and / or alternating manner. At the same time, by changing the inoculation propulsion state of the driving structure from the original one-spray-to-the-end working stroke to a controllable intermittent propulsion inoculation working state, and in conjunction with the intermittent working state of multiple control units, the trajectory of the liquid inoculation in the inoculation bag, which is the continuous and uninterrupted characteristic of the existing bulk material inoculation device, can be directly changed. The driving structure includes a servo motor 22, a transmission screw 23, and a liquid control plate 24. The output shaft of the servo motor 22 is connected to the transmission screw 23. The transmission screw 23 is arranged parallel to the liquid storage tube 5. The liquid control plate 24 is sleeved on the outer periphery of the transmission screw 23 and threadedly connected to the transmission screw 23. The liquid control plate 24 is magnetically coupled to the actuating element. As the liquid control plate 24 moves outside the liquid storage tube 5, the actuating element moves inside the liquid storage tube 5, thereby acting on the liquid bacteria inside the liquid storage tube 5. Meanwhile, due to the magnetic coupling between the liquid control plate 24 and the actuating elements in each liquid storage tube 5, multiple actuating elements can be linked together, enabling the coordinated operation of each inoculation structure.

[0026] In this embodiment, when the trajectory of the liquid bacteria needs to be arranged in a triangular equidistant pattern, two driving structures can be used to drive two sets of inoculation structures to output the liquid bacteria, and the two driving structures can work alternately. At this time, the trajectory of the liquid bacteria can be arranged in a triangular equidistant pattern.

[0027] In this embodiment, a first pneumatic valve body 18 is provided at one end of the liquid storage pipe 5, and a first valve body actuator 19 is provided at the first pneumatic valve body 18. A second pneumatic valve body 20 is provided at the other end of the liquid storage pipe 5, and a second valve body actuator 21 is provided at the second pneumatic valve body 20. Both the first valve body actuator 19 and the second valve body actuator 21 are solenoid valves.

[0028] The inoculation structure also includes a support frame, comprising a first fixing plate 25, a second fixing plate 26, and several fixing rods 27. The first fixing plate 25 and the second fixing plate 26 are arranged opposite to each other. The fixing rods 27 can pass through the first fixing plate 25, the liquid control plate 24, and the second fixing plate 26, and are fixedly connected to the first fixing plate 25 and the second fixing plate 26 respectively. The liquid storage tube 5 is fixedly connected to the first fixing plate 25 and the second fixing plate 26 respectively. The liquid control plate 24 is located between the first fixing plate 25 and the second fixing plate 26, and is slidably connected to the fixing rods 27 and the liquid storage tube 5 respectively. The transmission screw 23 is rotatably connected to the first fixing plate 25 and the second fixing plate 26 respectively. The fixing rods 27 and the liquid storage tube 5 can simultaneously serve as guides when the liquid control plate 24 slides. The transmission screw 23 is rotatably connected to the first fixing plate 25 and the second fixing plate 26 respectively via bearings.

[0029] The inoculator includes a spore delivery structure and an inoculation structure. The inlet of the spore delivery structure is connected to the outlet of the outlet tube assembly, and the spore delivery structure is used to transport liquid inoculum to the inoculation structure. The inoculation structure is used to inoculate the substrate in the center of the substrate bag in various dimensions. In this embodiment, when a single-unit design with six tubes is adopted, the spore delivery structure and the inoculation structure are basically the same as those in the prior art. A spiral blade 30 is provided on the outside of the spore delivery shaft 28 of the spore delivery structure, which can be used to transport the substrate during the inoculation process. A spore delivery channel 35 is provided inside the spore delivery shaft 28. Furthermore, in this embodiment, the inoculation structure is a multi-dimensional inoculation structure, and those skilled in the art can adapt its structure according to actual needs.

[0030] The inoculator also includes a material cylinder 29, which is a hollow cylinder with openings at both ends. The material cylinder 29 is located around the inoculation and feeding structures, with gaps between it and both structures. A flange 34 is provided at one end of the material cylinder 29, allowing it to be fixed to the outlet of the feed hopper. Spiral blades 30 on the feeding shaft 28 are located within the feed hopper and the material cylinder 29. As the feeding shaft 28 rotates, the sterilized and cooled substrate in the feed hopper is conveyed to the material cylinder 29 by the spiral blades 30, and continuously pressed forward into the plastic bag through the opening at the front end of the material cylinder 29. Simultaneously, multiple control units 31 located around the outer periphery of the material cylinder 29 discharge material according to a programmed trajectory.

[0031] Multiple control units 31 are arranged around the outer periphery of the material cylinder 29. Each control unit 31 includes a trajectory control valve 33 and a bag surface inoculum outlet pipe 32. The bag surface inoculum outlet pipe 32 is installed on the outer wall of the material cylinder 29. The first end of the bag surface inoculum outlet pipe 32 is connected to the sterilization port of the inoculum outlet pipe assembly. The trajectory control valve 33 is located near the first end of the bag surface inoculum outlet pipe 32 and is used to control whether the inoculum outlet pipe assembly delivers liquid inoculum into the bag surface inoculum outlet pipe 32. The second end of the bag surface inoculum outlet pipe 32 extends to the outlet of the material cylinder 29 and is used to inoculate the inner and outer layers of the substrate on the surface of the inoculum bag. By controlling the intermittent opening of each trajectory control valve 33, controllable quantitative intermittent inoculation is achieved. In this embodiment, the trajectory control valve 33 is a trajectory control solenoid valve, a trajectory control semi-automatic valve, or a trajectory control manual valve. The inoculation structure 2 includes an inoculation tube 37 and a storage tank 36. One end of the inoculation tube 37 is connected to the storage tank 36, and the bottom of the storage tank 36 is connected to the supply tube 4. When the main valve 38 is opened, the liquid bacteria in the storage tank 36 can fall into the supply tube 4 under the action of gravity.

[0032] In this embodiment, the control unit 31 also includes a shut-off valve, which is used to limit the pressure value at various points of the intermittent inoculation trajectory control device with controllable changes in the amount of liquid bacteria, thereby protecting the entire device when no drive structure is provided. In this embodiment, the shut-off valve is preferably a high-pressure pneumatic shut-off valve, and the trajectory control valve 33 is preferably a high-frequency solenoid valve.

[0033] Example 2 This embodiment provides a method for controlling the intermittent inoculation trajectory of liquid microbial inoculum with controllable quantitative variation. Specifically, it addresses the liquid microbial inoculation stage of existing bulk material briquette manufacturing processes, using the intermittent inoculation trajectory control device 1 with controllable quantitative variation of liquid microbial inoculum from Embodiment 1, including the following scenarios: 1. Liquid culture replenishment state, also the initial state (liquid culture enters the storage chamber 36 through the inlet tube 37 and is maintained at a pressure of 1.5 Bar): Liquid bacteria are introduced into the storage tank 36 through the inlet tube 37. The second valve 9 is opened. At the same time, the servo motor 22 drives the liquid control plate 24 to move towards the second fixed plate 26 and return to the zero position. The liquid bacteria pass through the storage tank 36, the inlet tube 4 and the second valve 9 in sequence to the storage tube 5. 2. Liquid inoculation status: The liquid inoculation in the storage tube 5 is introduced into each trajectory control valve 33, and the corresponding trajectory control valve 33 is opened according to the inoculation trajectory setting. At the same time, the liquid inoculation continues to be replenished into the storage tube 5 through the storage chamber 36. In the liquid inoculation state, when liquid inoculum is introduced into the storage tube 5, and the pressure in the storage tube 5 is intermittent and adjustable according to the interval setting value, inoculation within the culture bag can be achieved without a drive structure. That is, liquid inoculum is injected into the storage tube 5, and then pressure (e.g., 5 MPa) is applied to the storage tube 5. When intermittent injection of liquid inoculum into the culture bag is required, simply control the opening and closing frequency of each trajectory control valve 33 to achieve intermittent operation. Simultaneously, when continuously inoculating into the culture bag, maintaining pressure balance within the storage tube 5 is sufficient, simplifying the operation. It can perform intermittent work, and the trajectory interval of the liquid spawn can be set according to the actual parameters of the incubation time of the spawn bags. The trajectory of the liquid spawn includes at least the following: a triangular equidistant arrangement (as in Application Examples 3 and 4 below, this trajectory has the highest rationality, is the most scientific, and has the best effect), a parallel intermittent arrangement (as in Application Examples 1 and 2 below, the inoculation lines on the bag surface are parallel and corresponding from left to right, or the left and right sides are of different lengths), and an asymmetrical arrangement. However, in this embodiment, the inoculation trajectory of the liquid spawn is not limited to the above limitations. Those skilled in the art can also extend other types of inoculation trajectories based on conventional transformations. In this embodiment, the liquid bacterial inoculation states include an extended state and a retracted state, wherein... (1) Extended state (liquid control panel 24 extended). Close the second valve 9, open the first valve 8, the servo motor 22 drags the liquid control plate 24 toward the second fixed plate 26, at the same time, open the fourth valve 15, the liquid inoculum passes through the storage tube 5 in sequence through the fourth valve 15, the second inoculum outlet tube 11, the third inoculum outlet tube 12 and the fifth valve 16 to reach each trajectory control valve 33, and open the corresponding trajectory control valve 33 according to the inoculation trajectory setting. The liquid bacterial culture is sequentially delivered from the storage tank 36 through the supply tube 4, the first valve 8 and the first delivery tube 6 into the storage tube 5, thereby replenishing the liquid bacterial culture into the storage tube 5. (2) Retracted state (liquid control panel 24 retracts). Close the first valve 8, open the second valve 9, and the servo motor 22 drags the liquid control plate 24 toward the first fixed plate 25. At the same time, open the third valve 14. The liquid inoculum flows from the storage tube 5 through the first outlet tube 10, the third valve 14, the second outlet tube 11, the third outlet tube 12 and the fifth valve 16 to each trajectory control valve 33. Open the corresponding trajectory control valve 33 according to the inoculation trajectory setting. The liquid inoculum is delivered from the storage tank 36 through the inoculum supply pipe 4, the second valve 9, and the second inoculum transfer pipe 7 into the storage pipe 5, thereby replenishing the liquid inoculum into the storage pipe 5. In both the extended and retracted states, when the intermittent inoculation trajectory control device for controllable quantitative change of liquid culture includes a drive structure, and both the inoculation structure and the drive structure are a set, the servo motor 22 is controlled to complete the intermittent working state, and / or each trajectory control valve 33 is controlled to complete the intermittent working state. When both the inoculation structure and the drive structure are two sets, the servo motor 22 is controlled to complete the alternating working state, and / or each trajectory control valve 33 is controlled to complete the intermittent working state. During this process, the trajectory interval distance of the liquid culture, the density of the inoculation trajectory, and the density of the inoculation dimension are set according to the actual parameters of the culture bag incubation time, thereby determining the culture bag incubation cycle. For example, the distance between each culture point is 2cm-3cm, or it can be adjusted according to technical requirements.

[0034] This embodiment also includes an auxiliary control state. The pressure values ​​at various points of the intermittent inoculation trajectory control device 1, which uses a shut-off valve or overflow valve to limit the controllable change in the amount of liquid bacteria, complete the setting of the inoculation trajectory, and protect the safety of the components.

[0035] This embodiment, while maintaining the basic structure of the existing bulk material bagging machine inoculation device, adds a novel program control scheme to the bagging machine's spawn dispensing cylinder electrically controlled propulsion spawn injection system. This changes the servo motor 22's spawn injection propulsion state from a single, continuous spray to a controllable, intermittent propulsion spawn injection state. This directly alters the continuous, uninterrupted liquid inoculation trajectory within the spawn bag, as characteristic of existing bulk material inoculation devices, creating a novel, controllable, intermittent, and unique inoculation trajectory of liquid spawn within the edible mushroom spawn bag. Furthermore, it solves the following problems: (1) It solves the problem of excessive inoculation amount in the existing bulk material spawn making process. For example, the inoculation amount of conventional shiitake mushroom spawn (empty bag size 60mm×18mm, full bag size 45mm×12mm) in the existing bulk material process is an average of 220ml / spawn. However, the latest solution reduces the inoculation amount by at least 50% and the amount of inoculated liquid will be as low as 110ml / spawn due to the use of intermittent inoculation control program. (2) Since the solution in this embodiment can save at least 50% of the liquid inoculum volume in the spawn bag compared to the existing bulk material spawn making process, the cost of producing liquid spawn in the latest inoculation control solution is reduced by at least 50%; (3) The inoculation amount and inoculation trajectory can be controlled at will. In this embodiment, the inoculation trajectory travel distance of the bacterial liquid tank can be arbitrarily set by changing the propulsion program and working state of the servo motor 22 according to the process requirements, thereby determining the spacing and inoculation amount of the intermittent inoculation lines in the bacterial bag. The longer the spacing of the inoculation lines, the less the inoculation amount. The inoculation amount is the least when the same inoculation lines are in a triangular intersection trajectory. (4) Reducing quantity without reducing efficiency: The existing bulk material briquette process requires too much liquid inoculation (the traditional liquid single-sided inoculation amount for large mushroom briquettes is generally around 60ml). The existing bulk material process has an average inoculation amount of 220ml / briquette, while the bulk material briquette process in this embodiment has an average liquid inoculation amount of only 110ml / briquette, which is as close as possible to the inoculation amount of the traditional single-sided inoculation, but still maintains the special technological advantage of the bulk material process of fast mycelial growth. (5) Since the liquid inoculation control scheme in this embodiment can reduce the inoculation amount by at least 50%, the volume of the terminal culture tank used for liquid culture is reduced by at least 50%, and the cost of the terminal culture inoculation tank is reduced by at least 60%, thereby directly saving the huge investment cost of the terminal culture tank. (6) This embodiment can achieve multiple inoculation trajectories, including but not limited to parallel intermittent pattern, triangular equidistant arrangement and various asymmetrical patterns; (7) This embodiment can substantially improve the germination quality of the mushroom bags, making the surface environment of the mushroom bags more suitable for the growth conditions of edible fungi. One of the characteristics of bulk inoculation is that while inoculating the liquid spawn inside, the spawn is also inoculated onto the outer surface of the substrate of the mushroom bag. Since the liquid spawn is a liquid culture medium containing sugar with a comprehensive ratio of chemicals and grains, the large amount of liquid spawn inoculated onto the surface of the culture medium inside the mushroom bag will directly lead to an increase in monosaccharides on the surface of the substrate, making the culture environment more acidic and creating the best infection environment for various miscellaneous fungi. At the same time, the mycelial balls and mycelial fragments with germination ability of the liquid spawn will germinate after colonization. The initial mycelium is relatively weak and needs to adapt for 24 hours before gradually becoming robust. Therefore, the more liquid inoculation points on the substrate surface, the more weak microbial communities on the substrate surface, and the worse the mycelial quality. The bag surface is also the initial point of contamination by miscellaneous microorganisms. At the same time, the amount of inoculum introduced by the liquid solution is reduced by several times, which directly reduces the amount of liquid inoculum injected into the outer layer of the substrate inside the bag. This creates more space for the mycelium to move and rejuvenate. While reducing the increase of monosaccharides in the substrate on the bag surface, it effectively increases the robustness of the mycelium, making the overall process more perfect and more in line with the growth law of fungi and the process management requirements of mycotoxin cultivation and fruiting period.

[0036] Application Example 1 like Figures 9-10 As shown, this application embodiment uses the intermittent inoculation trajectory control device 1 with controllable quantitative change of liquid spawn in Embodiment 1 and the intermittent inoculation trajectory control method with controllable quantitative change of liquid spawn in Embodiment 2 to inoculate shiitake mushroom logs with liquid spawn. The inoculation trajectory is parallel and intermittent.

[0037] Application Example 2 like Figures 11-12 As shown, this application embodiment uses the intermittent inoculation trajectory control device 1 with controllable quantitative change of liquid spawn in Embodiment 1 and the intermittent inoculation trajectory control method with controllable quantitative change of liquid spawn in Embodiment 2 to inoculate black fungus spawn bags with liquid spawn. The inoculation trajectory is parallel and intermittent.

[0038] Application Example 3 like Figures 13-14 As shown, this application embodiment uses the intermittent inoculation trajectory control device 1 with controllable quantity change of liquid spawn in Embodiment 1 and the intermittent inoculation trajectory control method with controllable quantity change of liquid spawn in Embodiment 2 to inoculate shiitake mushroom logs with liquid spawn. The inoculation trajectory is a triangular intermittent pattern.

[0039] Application Example 4 like Figures 15-16 As shown, this application embodiment uses the intermittent inoculation trajectory control device 1 with controllable quantity change of liquid spawn in Embodiment 1 and the intermittent inoculation trajectory control method with controllable quantity change of liquid spawn in Embodiment 2 to inoculate black fungus spawn bags with liquid spawn. The inoculation trajectory is a triangular intermittent pattern.

[0040] Specific examples have been used to illustrate the principles and implementation methods of this invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this invention. Furthermore, those skilled in the art will recognize that, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. A method for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative changes, characterized in that: A discontinuous inoculation trajectory control device with controllable quantitative changes in liquid inoculum is used, including liquid inoculum replenishment and liquid inoculation states, wherein... The liquid culture replenishment status is as follows: the liquid culture in the storage tank (36) is introduced into the storage pipe (5); The liquid inoculation state is as follows: the liquid inoculation in the storage tube (5) is introduced into each trajectory control valve (33), and the corresponding trajectory control valve (33) is opened according to the inoculation trajectory setting. At the same time, the liquid inoculation continues to be replenished into the storage tube (5) through the storage chamber (36). When liquid culture is inoculated, the trajectory control valve (33) can perform intermittent operation when liquid culture is introduced into the storage tube (5), and the pressure of the storage tube (5) can be adjusted according to the spacing setting value. The trajectory interval distance of the liquid culture is set according to the actual parameter requirements of the culture bag incubation time. The trajectory of the liquid culture includes at least a triangular equidistant arrangement, a parallel intermittent arrangement, and an asymmetrical arrangement.

2. The method for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative change according to claim 1, characterized in that: The liquid culture replenishment state is the initial state. The liquid culture inoculation state includes the extended state and the retracted state. The extended state is as follows: the liquid bacteria in the storage tube (5) are introduced into each trajectory control valve (33) through the first route, the corresponding trajectory control valve (33) is opened according to the inoculation trajectory setting, and the liquid bacteria continue to be replenished into the storage tube (5) through the storage chamber (36); The retracted state is as follows: the liquid bacteria in the storage tube (5) are introduced into each trajectory control valve (33) through the second route, the corresponding trajectory control valve (33) is opened according to the inoculation trajectory setting, and the liquid bacteria continue to be replenished into the storage tube (5) through the storage chamber (36); In the extended and retracted states, when the intermittent inoculation trajectory control device for controllable quantitative change of liquid bacteria includes a drive structure, and the inoculation structure and the drive structure are both a set, the drive structure is controlled to complete the intermittent working state, and / or each trajectory control valve (33) is controlled to complete the intermittent working state. When the inoculation structure and the drive structure are both two sets, the drive structure is controlled to complete the alternating working state, and / or each trajectory control valve (33) is controlled to complete the intermittent working state.

3. The method for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative change according to claim 2, characterized in that: In the initial state, the method to introduce the liquid bacteria in the storage tank (36) into the storage pipe (5) is as follows: the liquid bacteria are introduced into the storage tank (36) through the inlet pipe (37), the second valve (9) is opened, and at the same time the drive structure drags the liquid control plate (24) to move back to the zero position in the direction of the second fixed plate (26). The liquid bacteria pass through the storage tank (36), the supply pipe (4) and the second valve (9) in sequence into the storage pipe (5).

4. The method for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative change according to claim 2, characterized in that: When in the extended state, the method to make the liquid inoculum reach each trajectory control valve (33) via the first route is as follows: close the second valve (9), open the first valve (8), drive the liquid control plate (24) to move towards the second fixed plate (26), and at the same time, open the fourth valve (15). The liquid inoculum passes through the storage tube (5) sequentially through the fourth valve (15), the second outlet tube (11), the third outlet tube (12) and the fifth valve (16) to reach each trajectory control valve (33). Open the corresponding trajectory control valve (33) according to the inoculation trajectory setting.

5. The method for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative change according to claim 2, characterized in that: When in the extended state, the method of replenishing the liquid bacteria from the storage tank (36) to the storage tube (5) is as follows: the liquid bacteria are sequentially fed from the storage tank (36) through the supply tube (4), the first valve (8) and the first delivery tube (6) into the storage tube (5), thereby replenishing the liquid bacteria to the storage tube (5).

6. The method for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative change according to claim 2, characterized in that: When in the retracted state, the method to make the liquid inoculum reach each trajectory control valve (33) via the second route is as follows: close the first valve (8), open the second valve (9), drive the liquid control plate (24) to move towards the first fixed plate (25), and at the same time, open the third valve (14). The liquid inoculum passes through the storage tube (5) in sequence through the first outlet tube (10), the third valve (14), the second outlet tube (11), the third outlet tube (12) and the fifth valve (16) to reach each trajectory control valve (33). Open the corresponding trajectory control valve (33) according to the inoculation trajectory setting.

7. The method for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative change according to claim 2, characterized in that: When the liquid culture is in the retracted state, the method of replenishing the liquid culture into the storage tube (5) through the storage tank (36) is as follows: the liquid culture is sequentially fed from the storage tank (36) through the supply tube (4), the second valve (9) and the second delivery tube (7) into the storage tube (5), thereby replenishing the liquid culture into the storage tube (5).

8. The method for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative change according to claim 1, characterized in that: It also includes auxiliary control status, which is as follows: By using shut-off valves or overflow valves to limit the pressure values ​​at various points of the intermittent inoculation trajectory control device that controls the amount of liquid bacteria, the inoculation trajectory can be set and the safety of the components can be protected.

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