Composition, essence and shampoo for preventing hair loss and densification

Abstract

The invention discloses a hair loss prevention and hair densification composition, relates to the technical field of washing and care formulas, and particularly relates to a hair loss prevention and hair densification auxiliary hair loss prevention composition, essence and shampoo. The composition comprises the following components in percentage by weight: 0.01%-50% of XVII type collagen; 0.1%-6% of a yeast extract; 0.01% to 4% of III type collagen; 1%-50% of a polypeptide compound; the polypeptide compound comprises oligopeptide 1, palmitoyl tripeptide 1, acetyl tetrapeptide 3 and acetyl heptapeptide 4; the anti-hair loss and dense hair essence and shampoo containing the composition also respectively comprise a water-based excipient and a corresponding functional auxiliary material. According to the composition for preventing hair loss and densification, through synergistic compatibility of the four components, multi-element synergistic intervention on hair follicle stem cell homeostasis, hair papilla cell energy metabolism, signal channels and extracellular matrixes is achieved, hair follicle microminiaturization is remarkably improved, the growth period is prolonged, the hair density is increased, and the composition has the excellent hair loss and densification preventing effect.

Description

Technical Field

[0001] This application relates to the field of hair care formulation technology, and in particular to a composition, serum and shampoo that prevents hair loss and promotes thicker hair. Background Technology

[0002] Hair loss is a common skin appendage disease, with androgenetic alopecia (AGA) being the most prevalent. Epidemiological data shows that the prevalence of AGA in Chinese men is approximately 21.3%, and in women approximately 6.0%, with a trend towards affecting younger people. Although hair loss does not directly threaten life, it severely impacts patients' appearance and mental health, often leading to anxiety, low self-esteem, and other emotional disorders. It has become a significant problem urgently needing to be addressed in the fields of dermatology and cosmetic medicine.

[0003] The pathological mechanisms of androgenetic alopecia involve multiple factors, including genetic susceptibility, abnormal androgen metabolism, and accelerated hair follicle miniaturization. Currently, globally approved anti-hair loss drugs mainly include topical minoxidil and oral finasteride. Minoxidil promotes hair growth by opening potassium channels, promoting vasodilation, and cell proliferation, but its onset of action is slow (usually requiring continuous use for 3-6 months), and newly grown hair gradually falls out after discontinuation. Finasteride reduces serum and scalp dihydrotestosterone levels by inhibiting type II 5α-reductase, but carries the risk of systemic adverse reactions such as decreased libido and erectile dysfunction, and some individuals do not respond well. These limitations have prompted researchers to continuously explore alternative or complementary treatments with novel mechanisms of action and higher safety profiles.

[0004] In recent years, the functional upgrading of scalp care products has become an important development direction for the cosmetics industry. However, how to achieve multi-dimensional synergistic intervention on hair follicle stem cell homeostasis, dermal papilla cell metabolic function, extracellular matrix integrity, and hair follicle cycle regulation through the rational combination of various active ingredients remains a technical bottleneck that urgently needs to be overcome in this field. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a multi-dimensional synergistic intervention technology for preventing hair loss and promoting hair growth by regulating the homeostasis of hair follicle stem cells, the metabolic function of hair papilla cells, the integrity of the extracellular matrix and the hair follicle cycle.

[0006] The objective of this invention is achieved through the following technical solution: According to one aspect of the present invention, a hair loss prevention and hair growth composition is provided, comprising, by weight of 100%, the following components: 0.01% to 50% type XVII collagen; 0.1% to 6% yeast extract; 0.01% to 4% type III collagen; and 1% to 50% polypeptide complex; wherein the polypeptide complex comprises oligopeptide-1, palmitoyl tripeptide-1, acetyl tetrapeptide-3, and acetyl heptapeptide-4.

[0007] Specifically, based on the total weight of the polypeptide complex as 100%, the contents of each active peptide in the polypeptide complex are as follows: Oligopeptide-1 0.03%~0.05%; Palmitoyl Tripeptide-1 0.015%~0.030%; Acetyl Tetrapeptide-3 0.0001%~0.0006%; Acetyl Heptapeptide-4 0.0001%~0.0005%.

[0008] When the above composition is formulated into a shampoo and conditioner product, based on the total weight of the shampoo and conditioner product as 100% of the total weight: the amount of type XVII collagen added is 0.001% to 5%; the amount of yeast extract added is 0.01% to 0.6%; the amount of type III collagen added is 0.001% to 0.4%; and the amount of polypeptide complex added is 0.1% to 5%.

[0009] Specifically, based on the total weight of the shampoo and conditioner product as 100%, the amount of type XVII collagen added is 4%; the amount of yeast extract added is 0.3%; the amount of type III collagen added is 0.2%; and the amount of polypeptide complex added is 4%. The polypeptide complex, based on its total weight as 100%, comprises: oligopeptide-1 0.043%; palmitoyl tripeptide-1 0.02%; acetyl tetrapeptide-3 0.0002%; and acetyl heptapeptide-4 0.0001%.

[0010] More specifically, peptide complexes also include water, polyols, organic acids, and surfactants.

[0011] More specifically, the polyols include at least one of butanediol, propylene glycol, pentanediol, hexanediol, and glycerol; and the organic acids include at least one of lactic acid, malic acid, and citric acid.

[0012] According to another aspect of this application, an anti-hair loss and hair growth serum is provided, comprising the above-described composition and an aqueous excipient; the aqueous excipient comprises water, a thickener, a chelating agent, a nonionic surfactant, a polyol, a penetration enhancer, and a preservative.

[0013] Specifically, the thickener is selected from at least one of sodium polyacrylamide dimethyl taurate, acrylate / C10-30 alkanol acrylate crosspolymer, xanthan gum, and carbomer; the nonionic surfactant is selected from at least one of PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, polysorbate-20, and polysorbate-80; the polyol is selected from at least one of 1,2-pentanediol, 1,2-hexanediol, glycerol, butylene glycol, and propylene glycol; the penetration enhancer is selected from at least one of laurocapram, menthol, propylene glycol, and ethanol; and the preservative is selected from at least one of ethylhexylglycerin, phenoxyethanol, octyl glycol, and p-hydroxyacetophenone.

[0014] According to another aspect of this application, an anti-hair loss and hair growth shampoo is provided, comprising the above-described composition and an aqueous excipient; the aqueous excipient comprises water, anionic surfactant, amphoteric surfactant, nonionic surfactant, thickener, chelating agent and preservative.

[0015] Specifically, the anionic surfactant is selected from at least one of sodium lauryl ether sulfate, sodium lauryl sulfate, sodium α-alkenyl sulfonate, and sodium lauroyl sarcosinate; the amphoteric surfactant is selected from at least one of cocamidopropyl betaine, sodium lauroyl sarcosinate, and sodium cocoamphoacetate; the nonionic surfactant is selected from at least one of cocamide MEA, cocamide DEA, lauryl glucoside, and decyl glucoside; the thickener is selected from at least one of guar hydroxypropyltrimethylammonium chloride, sodium chloride, polyquaternium-10, polyquaternium-7, and carbomer; the chelating agent is disodium EDTA or its salt; and the preservative is selected from at least one of phenoxyethanol, sodium benzoate, potassium sorbate, caprylyl glycol, and p-hydroxyacetophenone.

[0016] The beneficial effects achieved by this invention are as follows: This application scientifically combines type XVII collagen, type III collagen, yeast extract, and a polypeptide complex containing oligopeptide-1, palmitoyl tripeptide-1, acetyl tetrapeptide-3, and acetyl heptapeptide-4, achieving for the first time a four-dimensional synergistic anti-hair loss mechanism of "stabilizing hair follicle stem cells, enhancing energy metabolism, unlocking the Wnt signaling pathway, and strengthening the extracellular matrix." Each component complements and enhances the effects on targets such as stem cell niche protection, mitochondrial ATP production, DKK-1 inhibition, microcirculation improvement, and strengthening of hair follicle anchoring structures, which is significantly superior to single components or simple combinations. It can effectively delay hair follicle miniaturization, prolong the hair follicle growth phase, increase hair density and hair strength, and improve the scalp microenvironment. This composition can be flexibly adapted into various scalp care formulations such as serums and shampoos, with good formulation compatibility and significant social and commercial value. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a statistical table of hair loss counts at various time points before and after product use, and a statistical table of the difference in hair loss counts at each time point, based on an anti-hair loss and hair thickening essence in Embodiment 2 of this application. Figure 2 This is a statistical table of local hair density and local hair density difference between different time points before and after product use in an anti-hair loss and hair thickening essence test according to Embodiment 2 of this application. Figure 3 This is a statistical table of the overall hair density at various time points before and after product use, and a statistical table of the overall hair density difference at each time point, in an anti-hair loss and hair thickening essence test according to Embodiment 2 of this application. Figure 4 This is a summary table of the parameters of the test substance before and after use, and a summary table of the difference results of each parameter of the test substance, in the anti-hair loss and hair thickening shampoo of Embodiment 3 of this application. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this application clearer, the technical solutions of this application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of this application. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0020] Example 1 One method of implementing the hair loss prevention and hair growth composition of this application, based on 100% of the total weight of the composition, includes the following components: Type XVII collagen: 0.01%–50%; Yeast extract 0.1%–6%; Type III collagen 0.01%–4%; Peptide complex 1%–50%; The active peptide components of the polypeptide complex include oligopeptide-1, palmitoyl tripeptide-1, acetyl tetrapeptide-3, and acetyl heptapeptide-4. Based on the total weight of the polypeptide complex (100%), the content of each active peptide in the polypeptide complex is as follows: Oligopeptide-1 0.03%~0.05%; Palmitoyl tripeptide-1 0.015%~0.030%; Acetyl tetrapeptide-3 0.0001%~0.0006%; Acetyl heptapeptide-4 0.0001%~0.0005%.

[0021] Specifically, polypeptide complexes also include water, polyols, organic acids, and surfactants.

[0022] More specifically, the polyols include at least one of butanediol, propylene glycol, pentanediol, hexanediol, and glycerol; and the organic acids include at least one of lactic acid, malic acid, and citric acid.

[0023] In this embodiment, type XVII collagen is a key transmembrane protein of hair follicle stem cells (HFSCs), comprising collagen: 5.0-6.0% and mannitol: 94-95%.

[0024] The main methods to prevent hair loss and increase hair density are as follows: Stabilizing hair follicle stem cells: By regulating signaling pathways such as Notch and c-MYC, it delays the aging of hair follicle stem cells, prevents hair follicle miniaturization and disappearance, and maintains the cycle of hair regeneration from the source.

[0025] Antioxidant stress: Significantly reduces the content of reactive oxygen species (ROS) and reduces the damage of oxidative stress to hair follicle cells.

[0026] Regulates inflammatory response: Inhibits the secretion of pro-inflammatory factors IL-6 and IL-1β, reducing the damage of scalp inflammation to hair follicles.

[0027] Regulating hair loss-related pathways: Inhibiting the negative regulator of the Wnt pathway, DKK 1. The expression of SFRP2 and PEDF upregulates β-catenin gene expression, promoting dermal papilla cell proliferation; simultaneously, it inhibits TGF. Apoptosis-related genes such as β2 and EGLN1 / 3 reduce hair follicle cell apoptosis.

[0028] Promotes hair follicle regeneration: Animal experiments have shown that it can promote the regeneration of hair follicles in the hair removal area, and the effect is more significant when used in combination with type III collagen.

[0029] Specifically, the XVII type collagen uses ARHCXVIIPowder, with the following composition and ratio: Collagen: 5%; Mannitol: 95%.

[0030] Type III collagen is a core structural protein that builds the hair follicle microenvironment. It includes collagen (CAS9007-34-5): 4.0-6.0%; mannitol (CAS69-65-8): 94-95%.

[0031] The main methods to prevent hair loss and increase hair density are as follows: Constructing a supportive environment for hair follicles: It can self-assemble into triple-helix collagen fibers that are homologous to those in the human body, forming a structure with a diameter of hundreds of nanometers. It is easily absorbed through the skin and reaches the dermis, providing a natural extracellular matrix support space for hair follicle stem cells.

[0032] Promotes the production of multiple types of collagen: It can promote the production of various types of collagen, such as I, III, IV, V, VII, and XVII, to enrich the extracellular matrix around hair follicles, strengthen hair follicle anchoring, and reduce hair loss.

[0033] Repairing the hair follicle microenvironment: Enhancing cell migration and vitality, repairing the scalp barrier, improving microcirculation around hair follicles, and providing a good nutritional environment for hair growth.

[0034] Antioxidant and anti-degradation: Inhibits the activity of elastase and collagenase, reduces collagen fiber degradation, reduces ROS damage, and delays scalp aging-related hair loss.

[0035] Specifically, type III collagen uses micoreCol.ⅢSMART, with the following composition and ratio: collagen (CAS9007-34-5): 5%; mannitol (CAS69-65-8): 95%.

[0036] Yeast extract is a core energy-supplying factor for hair follicles, comprising yeast extract (YEASTEXTRACT): 46.00-51.00%; maltodextrin (MALTODEXTRIN): 44.00-49.00%; and water (WATER): 0.01-10.00%.

[0037] The main methods to prevent hair loss and increase hair density are as follows: Activates mitochondrial activity and supplies energy for hair follicle growth: Promotes the synthesis of mitochondrial fusion protein 1 in human dermal papillary fibroblasts (0.04% concentration can increase it by 52%), regulates the balance of mitochondrial fission and fusion, increases ATP production, provides sufficient energy for hair follicle growth, and improves the problem of insufficient energy supply to hair follicles in androgenetic alopecia.

[0038] Regulating hair loss-related signaling molecules and combating dihydrotestosterone damage: Significantly inhibiting the abnormal expression of key signaling molecules in androgenetic alopecia, a 0.04% concentration can limit the secretion of pro-inflammatory factor IL-6 by 97%, inhibit the expression of Wnt pathway inhibitor DKK-1 by 91%, and the expression of dermal papillary fibroblast senescence marker P16 by 71%, thereby relieving the inhibition of hair follicle growth and delaying hair follicle miniaturization.

[0039] Regulating epigenetic modification and repairing hair follicle growth pathway: restoring the expression of uncontrolled microRNAs induced by dihydrotestosterone; a 0.04% concentration can restore the expression of miR-3663-3p and let-7a-3p by 50% and 89%, respectively; targeting and regulating the Wnt / β-catenin pathway and repairing the normal hair follicle growth cycle.

[0040] Activate dermal papillary cell activity and maintain hair follicle growth phase: Promote the synthesis of pluripotent proteoglycans in the globular bodies of dermal papillary cells, restore the activity of dermal papillary fibroblasts, maintain the characteristics of hair follicle growth phase, reduce the number of hair follicles entering the regression and resting phases, and reduce the probability of hair loss.

[0041] Promotes hair follicle growth and hair density: In in vitro experiments, a 0.04% concentration can increase the growth of D5 and D7 hair follicles by 31% and 37% respectively, and maintain the proliferative capacity of keratinocytes; In vivo experiments show that continuous use of a 0.3% concentration for 8 months can increase hair density by 24.9%, increase the proportion of hair in the growth phase by 49.1%, and reduce the proportion of hair in the resting phase by 26.1%.

[0042] Limits abnormal hair loss and stabilizes scalp condition: Washing experiments have verified that using a 0.3% concentration for 8 months can reduce hair loss by 34%, balance the scalp microecology, and replenish hair follicles with active substances such as amino acids and peptides, strengthening hair root resilience and reducing hair breakage and shedding.

[0043] Specifically, the yeast extract uses Hairgenyl®, with the following composition and ratio: YEASTEXTRACT: 48.50% (typical); maltodextrin: 46.50% (typical); water: 5.00% (typical).

[0044] The polypeptide complex is a multifunctional regulator of hair follicle structure and microcirculation, and it includes the following components: Based on the total weight of the polypeptide complex as 100%, Active peptide ingredients: Oligopeptide-1 (OLIGOPEPTIDE-1): 0.03-0.05%; Acetyltetrapeptide-3: 0.0001-0.0006%; Palmitoyl tripeptide-1: 0.015-0.030%; Acetylheptapeptide-4: 0.0001-0.0005%; Excipient / solvent composition: 1,2-Hexanediol: 0.5-3.0%; 1,2-Pentyleneglycol: 1.0-5.0%; PPG-13-Decylttradecet-24: 0.5-3.0%; Glycerin: 1.0-40.0%; Citric acid: 0.02-0.05%; WATER: 48.8689-87.9348%.

[0045] Preferably, in this embodiment, based on the total weight of the polypeptide complex as 100%, the individual active peptide components it contains are as follows: Oligopeptide-1 0.043%; Palmitoyl tripeptide-1 0.02%; Acetyl tetrapeptide-3 0.0002%; Acetyl heptapeptide-4 0.0001%.

[0046] The main methods to prevent hair loss and increase hair density are as follows: Stabilizes the scalp environment: Inhibits oxidizing substances such as hydroxyl radicals (·OH) and peroxide radicals (ROO-), balances the scalp flora, and reduces hair loss caused by oxidative stress and flora imbalance.

[0047] Improve microcirculation: Promote the secretion of vascular endothelial growth factor (VEGF), enhance scalp blood circulation, improve the nutrient supply to hair follicles, and improve hair loss related to microcirculation disorders.

[0048] Protects hair follicle structure: Inhibits the activity of matrix metalloproteinases (MMP-2 / 9), reduces extracellular matrix loss, strengthens the connection between hair follicles and scalp, and reduces the risk of hair follicle loss.

[0049] Strengthens hair roots and strands: Promotes the production of type III collagen and laminin, filling hair follicles with volume; at the same time, it promotes keratin synthesis, strengthens hair quality, and reduces breakage and thinning.

[0050] Specifically, the polypeptide complex uses WKPep® MEDorder01, with typical proportions as follows: oligopeptide - 10.043%, acetyl tetrapeptide - 30.0002%, palmitoyl tripeptide - 10.02%, acetyl heptapeptide - 40.0001%, 1,2-hexanediol 1.0%, 1,2-pentanediol 3.0%, PPG-13-decyltetradecyl alcohol polyether - 241.0%, glycerol 3.0%, citric acid 0.04%, and water 64.8967%.

[0051] Furthermore, when the above composition is formulated into a shampoo and conditioner product, the corresponding amount of the additive is 1% to 5% based on the total weight of the shampoo and conditioner product: The addition amount of type XVII collagen is 0.001% to 5%; The amount of yeast extract added is 0.01% to 0.6%; The addition amount of type III collagen is 0.001% to 0.4%; The amount of peptide complex added is 0.1% to 5%.

[0052] Preferably, the amount of this additive is 4%, based on the total weight of the aforementioned shampoo and conditioner products. The addition amount of type XVII collagen is 4%; The amount of yeast extract added was 0.3%; The addition amount of type III collagen is 0.2%; The amount of peptide complex added was 4%; Among them, oligopeptide-1 was 0.00172%; Palmitoyl tripeptide-1 0.0008%; Acetyl tetrapeptide-3 0.000008%; Acetyl heptapeptide-4 0.000004%.

[0053] The core technological contribution of this invention lies in the fact that type XVII collagen, type III collagen, yeast extract, and the aforementioned polypeptide complex are not simply the sum of the functions of their components, but rather form a synergistic system covering the entire life cycle and multidimensional microenvironment of hair follicles through target complementarity, pathway cascading, and hierarchical enhancement. Specifically, the synergistic effect manifests in the following five aspects: 1. Synergy at the extracellular matrix (ECM) level – constructing “anchoring” support "Anti-degradation" integrated structure Type III collagen self-assembles into a triple-helix fiber network, directly providing mechanical support for hair follicle stem cells and inducing the synthesis of various ECM components, including type XVII collagen. Type XVII collagen, as a core protein of the hemidesmosome, strengthens the anchoring connection between hair follicle stem cells and the basement membrane. Simultaneously, type III collagen inhibits elastase / collagenase, and its polypeptide complex inhibits MMPs. 2 / 9, both block ECM degradation through different pathways; type XVII collagen further disrupts the cascade damage of "oxidative stress → protease activation → ECM loss" by reducing ROS. The four work synergistically to create a regenerative microenvironment for hair follicles that is structurally intact, firmly anchored, and significantly enhances its resistance to degradation.

[0054] 2. Synergistic regulation of core signaling pathways – Dual unlocking of the Wnt pathway and multidimensional inhibition of inflammation / androgen damage (1) Dual activation of the Wnt / β-catenin pathway Both yeast extract and type XVII collagen can significantly inhibit the potent inhibitor DKK of this pathway. 1. (Inhibition rates reached 91% and were significant, respectively). Type XVII collagen also additionally inhibited negative regulatory factors such as SFRP2 and PEDF, achieving "dual unlocking of pathways." Type III collagen enhanced the sensing efficiency of hair follicle stem cells to Wnt signals by optimizing ECM mechanical properties. The polypeptide complex improved microcirculation by promoting VEGF secretion and enhanced the efficiency of signal molecule delivery. The four components synergistically maximized β-catenin nuclear translocation, strongly activating the proliferation and differentiation of hair follicle stem cells.

[0055] (2) Multi-target inhibition of inflammation and androgenic alopecia pathway Both yeast extract and type XVII collagen can effectively inhibit the pro-inflammatory factor IL. 6. Type XVII collagen simultaneously inhibits IL. 1β; yeast extract can also inhibit DHT-induced DPC aging markers P16 and type XVII collagen, and inhibit TGF. Apoptosis-inducing genes such as β2 and EGLN1 / 3; the polypeptide complex reduces inflammatory triggers by balancing the scalp microbiota. This forms a comprehensive inhibitory network from "inflammatory mediators—cellular senescence—apoptotic signals—microbiota dysbiosis," significantly enhancing the ability to combat androgenetic alopecia.

[0056] 3. Synergy between energy supply and hair follicle vitality – a three-pronged approach combining mitochondrial energy supply, microcirculation energy transport, and cellular anti-aging. Yeast extract acts directly on mitochondria, promoting the synthesis of fusion protein 1 and increasing ATP production, fundamentally addressing the core defect of "energy deficiency" in hair follicle cells in androgenetic alopecia. Type III collagen and peptide complex synergistically promote VEGF secretion and improve microcirculation, ensuring sufficient delivery of energy substrates and oxygen. Type XVII collagen delays the aging of dermal papilla cells (DPCs) through the Notch pathway, maintaining their inducing activity for hair follicle growth. These three components constitute a complete energy metabolism enhancement chain of "energy production—energy transport—energy-consuming cell rejuvenation".

[0057] 4. Synergy between hair follicle stem cell homeostasis and hair regeneration cycle – “Maintaining stability” activation Output "three-level linkage" Level 1 – Stabilization: Type XVII collagen regulates the Notch / c-MYC pathway to delay the aging of hair follicle stem cells, while the ECM niche constructed by Type III collagen provides a protective environment for them. The antioxidant effect of yeast extract reduces ROS damage to stem cells. The three work together to maintain the long-term stability of the hair follicle stem cell pool.

[0058] Level 2 – Activation: Both yeast extract and type XVII collagen have been shown to promote hair follicle regeneration in the hair removal area, and their combined use significantly improves regeneration efficiency.

[0059] The third stage – output: The polypeptide complex promotes the synthesis of keratin and type III collagen, making regenerated hair thicker and more resistant to shedding; type III collagen provides the scaffold needed for regeneration; yeast extract provides regenerative energy. These four elements form a complete regenerative output loop of "sufficient stem cell reserves – efficient regeneration signals – strong new hair".

[0060] 5. Synergistic effect of scalp microenvironment balance – combining antioxidant, anti-inflammatory, and barrier repair effects. Antioxidant: Type XVII collagen reduces ROS, yeast extract inhibits oxidative damage, and peptide complex directly scavenge ·OH and ROO-, resulting in a triple antioxidant mechanism.

[0061] Anti-inflammatory: Yeast extract, type XVII collagen, and peptide complex can all inhibit IL-6. Type XVII collagen additionally inhibits IL-1β, and peptide complex balances the microbiome, controlling inflammation from both cytokine and microbial dimensions.

[0062] Barrier repair: Type III collagen repairs the scalp's physical barrier, reducing the activation of inflammatory pathways by external stimuli.

[0063] The three work together to break the vicious cycle of "oxidative stress → inflammation outbreak → barrier damage → aggravated hair loss" at its source.

[0064] Example 2 One implementation method of the anti-hair loss and hair growth essence of this application is basically the same as that of Embodiment 1. Features not explained in this embodiment are explained using the methods in Embodiment 1, and will not be repeated here. The difference between this embodiment and Embodiment 1 is: A hair loss prevention and hair growth serum is provided, comprising the above-mentioned composition and an aqueous excipient (i.e., a base carrier); wherein the aqueous excipient comprises water, a thickener, a chelating agent, a nonionic surfactant, a polyol, a penetration enhancer, and a preservative.

[0065] Specifically, the thickener is selected from at least one of sodium polyacrylamide dimethyl taurate, acrylate / C10-30 alkanol acrylate crosspolymer, xanthan gum, and carbomer; the nonionic surfactant is selected from at least one of PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, polysorbate-20, and polysorbate-80; the polyol is selected from at least one of 1,2-pentanediol, 1,2-hexanediol, glycerol, butylene glycol, and propylene glycol; the penetration enhancer is selected from at least one of laurocapram, menthol, propylene glycol, and ethanol; and the preservative is selected from at least one of ethylhexylglycerin, phenoxyethanol, octyl glycol, and p-hydroxyacetophenone.

[0066] In this embodiment, the essence comprises the following components in total:

[0067] I. Materials and Methods for the Anti-Hair Loss and Hair Growth Test 1. Test product: The serum with the above formula was used as the test product.

[0068] 2. Control product: The corresponding test product matrix formula product that does not contain anti-hair loss active ingredients was tested in parallel with the test product.

[0069] 3. Washout period product: Same as the control product, both the experimental group and the control group used the control product during the washout period.

[0070] 4. Subjects: A total of 76 subjects, including 1 male and 75 females, aged 20 to 56 years, with a mean age of 42.46 ± 7.39 years, meeting the subject voluntary inclusion criteria.

[0071] 5. Test Equipment: Professional digital camera: with a resolution of no less than 15 megapixels, maintaining consistent parameters including aperture, ISO, and focal length throughout the test; Image shooting stand: capable of fixing the subject's head position, keeping the top of the head perpendicular to the camera lens, and using the professional digital camera to take photos of all hair centered on the top of the head; Dermatoscope; LED light source, wavelength 450 nm. 750nm, color temperature 6500 8000K, illuminance not less than 540lx, magnification ≥20x, detection diameter not less than 1.0cm or area not less than 0.8cm². 2 Comb: The comb teeth have a moderate density (tooth spacing 0.9). 1.1mm), comb tooth length is 2.0. The comb should be 3.0cm long and at least 10cm long (excluding the handle). The same size and material of comb must be used throughout the test. After each use, disinfection should be carried out in accordance with the relevant requirements in the "Technical Specifications for Disinfection of Medical Institutions" (WS / T367-2012).

[0072] 6. Environmental conditions: Test results should be observed at a temperature of 21±1℃ and a relative humidity of 50±10%RH. Visual evaluation should be conducted under constant lighting conditions (color temperature 5500). 6500K5500 The assessment and testing should be conducted under 6500K fluorescent or LED lighting conditions, and subjects should acclimatize to these conditions for at least 30 minutes before the assessment and testing can proceed.

[0073] 7. Experimental Methods: The experiment was conducted in accordance with the specific requirements of the "Cosmetic Safety Technical Specifications" (2015 edition). Before enrollment, subjects were questioned about their medical history and health status according to the inclusion and exclusion criteria. The number of hairs lost was counted using the 60-comb test and recorded. Eligible subjects underwent a 2-week shampooing period. After the shampooing period, the 60-comb test was performed again. Only those with a hair loss count still greater than 10 strands were eligible for the formal trial. Subjects selected for the formal trial were randomly assigned to the test product group and the control product group using a stratified randomization method to ensure a balance of important factors that could affect the experimental results (gender, age, hair length, severity of hair loss, etc.). Staff distributed the test product and control product according to the randomization table and provided product usage guidance to subjects according to the instructions, ensuring that subjects used the test product correctly during the trial period. The test product was used for at least 12 weeks. During the trial, subjects were required to record the duration of use and any discomfort or adverse reactions during use. Pre-use hair baseline values ​​were assessed for enrolled subjects, including hair loss count, hair density assessment, and image capture, and the results were recorded. The same assessment and testing were repeated at 4, 8, and 12 weeks after product use.

[0074] 8. Data Statistics: Statistical analysis software was used to perform statistical analysis on the data. Quantitative data were expressed as mean ± standard deviation and subjected to a normality test. If the data met the normality requirement, paired t-tests were used for comparisons of the data before and after the same period; otherwise, a two-sample rank-sum test was used. Ordinal data were compared using a two-sample rank-sum test. Comparisons between the experimental product and the control group were performed using independent samples t-tests or rank-sum tests. All statistical analyses were two-tailed tests, with a significance level of α = 0.05.

[0075] 9. Conclusion: Based on the data analysis results, if at any visit time point during the trial period, there is no significant increase in hair loss count before and after using the test product, or the difference in hair loss count before and after (hair loss count at a certain visit time point after product use - hair loss count before product use) is significantly lower than that of the control group (P<0.05); or if there is no significant decrease in hair density (overall hair density or local hair density), or the difference in hair density before and after (hair density at a certain visit time point after product use - hair density before product use) is significantly higher than that of the control group (P<0.05), then the test product is considered to have anti-hair loss effect; otherwise, the test product is considered to have no anti-hair loss effect.

[0076] II. Data Analysis of Experimental Results 1. For specific test results of the measurement data, please refer to [link / reference]. Figure 1 and Figure 2 Tables 1, 2, 3 and 4 are included.

[0077] 2. For detailed inspection results of the graded data, please refer to [link / reference]. Figure 3 Tables 5 and 6 are included.

[0078] III. Experimental Conclusions After 4, 8, and 12 weeks of use of the experimental product, the difference in hair loss count before and after using the experimental product was significantly lower than that in the control group (P<0.05).

[0079] After 4, 8, and 12 weeks of use of the test product, the difference in local hair density before and after using the test product was significantly higher than that in the control group (P<0.05).

[0080] In conclusion, under the experimental conditions, the tested product demonstrated its effectiveness in preventing hair loss.

[0081] Example 3 One implementation method of the anti-hair loss and hair growth shampoo of this application is basically the same as that of Embodiment 1. Features not explained in this embodiment are explained using the methods in Embodiment 1, and will not be repeated here. The difference between this embodiment and Embodiment 1 is: A hair loss prevention and hair growth shampoo is provided, comprising the above-described composition and an aqueous excipient (i.e., a base carrier); the aqueous excipient comprises water, anionic surfactant, amphoteric surfactant, nonionic surfactant, thickener, chelating agent, and preservative.

[0082] Specifically, the anionic surfactant is selected from at least one of sodium lauryl ether sulfate, sodium lauryl sulfate, sodium α-alkenyl sulfonate, and sodium lauroyl sarcosinate; the amphoteric surfactant is selected from at least one of cocamidopropyl betaine, sodium lauroyl sarcosinate, and sodium cocoamphoacetate; the nonionic surfactant is selected from at least one of cocamide MEA, cocamide DEA, lauryl glucoside, and decyl glucoside; the thickener is selected from at least one of guar hydroxypropyltrimethylammonium chloride, sodium chloride, polyquaternium-10, polyquaternium-7, and carbomer; the chelating agent is disodium EDTA or its salt; and the preservative is selected from at least one of phenoxyethanol, sodium benzoate, potassium sorbate, caprylyl glycol, and p-hydroxyacetophenone.

[0083] More specifically, based on the total volume of this shampoo, its aqueous excipients include the following components: Sodium lauryl ether sulfate, content 10 25%; Cocamidopropyl betaine, content 3 20%; Sodium lauroyl sarcosinate, content 3 20%; Guar gum hydroxypropyltrimethylammonium chloride, content 0.05 0.5%; Cocamide MEA, content 0.5 2%; Phenoxyethanol, content 0.1 1%; Sodium benzoate, content 0.1 0.5%.

[0084] In this embodiment, the shampoo, in total volume, specifically comprises the following components:

[0085] I. Materials and Methods for the Anti-Hair Loss and Hair Growth Test 1. Test product: The shampoo with the above formula was used as the test product.

[0086] 2. Control product: The corresponding test product matrix formula product that does not contain anti-hair loss active ingredients was tested in parallel with the test product.

[0087] 3. Washout period product: Similar to the control product, both the test product group and the control product group used the control product during the washout period.

[0088] 4. Subjects: The experimental product group consisted of 32 subjects, including 1 male and 31 females, aged 30 to 45 years, with a mean age of 39.1 ± 4.2 years; the control product group consisted of 31 subjects, including 2 males and 29 females, aged 30 to 45 years, with a mean age of 36.5 ± 4.8 years; both groups met the subject voluntary inclusion criteria.

[0089] 5. Experimental Methods: The testing was conducted according to the specific requirements of the "Cosmetic Safety Technical Specifications" (2015 edition). Volunteer subjects were recruited based on inclusion and exclusion criteria. Qualified subjects underwent a 2-week washout period. After the washout period, a 60-times combing test was performed again. Only subjects with a hair loss count still greater than 10 strands were eligible for the formal trial. Subjects were randomly assigned to the experimental product group and the control product group using a stratified randomization method. Pre-product hair basal values ​​were assessed in subjects, including hair loss count, hair density assessment, and image capture, and the data were recorded. The product was used continuously for at least 12 weeks according to the instructions. The same assessment and testing were repeated at 4, 8, and 12 weeks after product use, and every 4 weeks thereafter (if applicable).

[0090] II. Test Results The test results are shown in Figure 4 Tables 1 and 2 are included.

[0091] III. Experimental Conclusions Compared with before use, after 4 weeks of using the tested sample, the local hair density increased significantly (P<0.05); after 8 weeks of using the tested sample, the hair loss count decreased significantly (P<0.05) and the local hair density increased significantly (P<0.05); after 12 weeks of using the tested sample, the hair loss count decreased significantly (P<0.05), the local hair density increased significantly (P<0.05), and the overall hair density increased significantly (P<0.05).

[0092] Compared with the control product, the difference in hair loss count after using the tested sample for 4, 8 and 12 weeks was significantly lower than that in the control group (P<0.05), and the difference in local hair density was significantly higher than that in the control group (P<0.05).

[0093] In conclusion, under the conditions of this experiment, the tested sample has the effect of preventing hair loss.

[0094] During this experiment, none of the 63 subjects experienced any adverse reactions related to the tested samples.

[0095] In summary, this application, through the systematic integration of type XVII collagen, type III collagen, yeast extract, and specific polypeptide complexes, has for the first time constructed a four-dimensional synergistic anti-hair loss technology platform encompassing "hair follicle stem cell stabilization—energy metabolism enhancement—Wnt signaling pathway unlocking—extracellular matrix reinforcement." Example 1 confirmed the complementary synergistic mechanism of the four components at the levels of molecular targets, signaling pathways, cellular function, and tissue microenvironment. Examples 2 and 3, using serum and shampoo as dosage forms respectively, conducted randomized, controlled, 12-week human efficacy trials, demonstrating that the composition of this application was significantly superior to the matrix control group without active ingredients in reducing the amount of hair loss and increasing local and overall hair density (P<0.05), with no adverse reactions related to the test products observed. The technical solution of this application completely solves the technical bottleneck of "difficulty in multi-dimensional synergistic intervention" pointed out in the background art, possessing outstanding substantive features and significant progress.

[0096] It should be noted that the above embodiments are merely preferred embodiments of this application and are not intended to limit the scope of protection of this application. Any equivalent substitutions, simple adjustments, or adaptive improvements made to the components, proportions, types of excipients, and dosage forms described in this application based on the technical solutions of this application shall be deemed to fall within the scope of protection of the claims of this application.

[0097] Note that the above are merely preferred embodiments and the technical principles employed in this application. Those skilled in the art will understand that this application is not limited to the specific embodiments described herein, and various obvious changes, readjustments, and substitutions can be made without departing from the scope of protection of this application. Therefore, although this application has been described in detail through the above embodiments, this application is not limited to the above embodiments, and may include many other equivalent embodiments without departing from the concept of this application, the scope of which is determined by the scope of the appended claims.

Claims

1. A composition for preventing hair loss and promoting hair growth, characterized in that, The composition comprises the following components based on 100% of its total weight: Type XVII collagen: 0.01%–50%; Yeast extract 0.1%–6%; Type III collagen 0.01%–4%; Peptide complex 1%–50%; The polypeptide complex comprises oligopeptide-1, palmitoyl tripeptide-1, acetyl tetrapeptide-3, and acetyl heptapeptide-4.

2. The composition for preventing hair loss and promoting hair growth according to claim 1, characterized in that... Based on the total weight of the polypeptide complex as 100%, the content of each active peptide in the polypeptide complex is as follows: Oligopeptide-1 0.03%~0.05%; Palmitoyl tripeptide-1 0.015%~0.030%; Acetyl tetrapeptide-3 0.0001%~0.0006%; Acetyl heptapeptide-4 0.0001%~0.0005%.

3. The composition for preventing hair loss and promoting hair growth according to claim 1 or 2, characterized in that: When the composition is formulated into a personal care product, it is based on 100% of the total weight of the personal care product: The addition amount of type XVII collagen is 0.001% to 5%; The amount of yeast extract added is 0.01% to 0.6%; The addition amount of type III collagen is 0.001% to 0.4%; The amount of peptide complex added is 0.1% to 5%.

4. The composition for preventing hair loss and promoting hair growth according to claim 3, characterized in that: Taking the total weight of the aforementioned hair care products as 100% as the time, The amount of type XVII collagen added is 4%; The amount of yeast extract added is 0.3%; The amount of type III collagen added is 0.2%; The amount of the polypeptide complex added is 4%; The polypeptide complex, by weight 100%, comprises: The oligopeptide-1 contains 0.043%; The palmitoyl tripeptide-1 was 0.02%; The acetyl tetrapeptide-3 is 0.0002%; The acetyl heptapeptide-4 is 0.0001%.

5. The composition for preventing hair loss and promoting hair growth according to claim 4, characterized in that: The polypeptide complex also includes water, polyols, organic acids, and surfactants.

6. The composition for preventing hair loss and promoting hair growth according to claim 5, characterized in that: The polyol includes at least one of butanediol, propylene glycol, pentanediol, hexanediol, and glycerol; The organic acid includes at least one of lactic acid, malic acid, and citric acid.

7. A hair loss prevention and hair growth serum, characterized in that, The composition comprises any one of claims 1 to 6, and an aqueous excipient; The aqueous excipients comprise water, thickeners, chelating agents, nonionic surfactants, polyols, penetration enhancers, and preservatives.

8. The anti-hair loss and hair growth serum according to claim 7, characterized in that: The thickener is selected from at least one of sodium polyacrylamide dimethyl taurate, acrylate / C10-30 alkanol acrylate crosspolymer, xanthan gum, and carbomer; The nonionic surfactant is selected from at least one of PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, polysorbate-20, and polysorbate-80; The polyol is selected from at least one of 1,2-pentanediol, 1,2-hexanediol, glycerol, butylene glycol, and propylene glycol; The penetration enhancer is selected from at least one of laurocapram, menthol, propylene glycol, and ethanol; The preservative is selected from at least one of ethylhexylglycerin, phenoxyethanol, octyl glycol, and p-hydroxyacetophenone.

9. A shampoo for preventing hair loss and promoting hair growth, characterized in that, The composition comprising any one of claims 1 to 6, and an aqueous excipient; The aqueous excipients comprise water, anionic surfactants, amphoteric surfactants, nonionic surfactants, thickeners, chelating agents, and preservatives.

10. The anti-hair loss and hair-thickening shampoo according to claim 9, characterized in that: The anionic surfactant is selected from at least one of sodium lauryl ether sulfate, sodium lauryl sulfate, sodium α-alkenyl sulfonate, and sodium lauroyl sarcosinate. The amphoteric surfactant is selected from at least one of cocamidopropyl betaine, sodium lauroyl sarcosinate, and sodium cocoamphoacetate. The nonionic surfactant is selected from at least one of cocamide MEA, cocamide DEA, lauryl glucoside, and decyl glucoside; The thickener is selected from at least one of guar hydroxypropyltrimethylammonium chloride, sodium chloride, polyquaternium-10, polyquaternium-7, and carbomer; The chelating agent is disodium EDTA or a salt thereof; The preservative is selected from at least one of phenoxyethanol, sodium benzoate, potassium sorbate, caprylyl glycol, and p-hydroxyacetophenone.

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