Insecticidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticides, and relates to an insecticidal composition and application thereof. The insecticidal composition comprises an active component A and an active component B, the active component A is a compound represented by a formula (I), the active component B is any one of metaflumizone and indoxacarb, and the mass ratio of the active component A to the active component B is 1: 54-43: 1. The composition disclosed by the invention has a synergistic effect on prevention and control of agricultural and forestry pests within a certain proportioning range, particularly has excellent insecticidal activity on lepidoptera pest larvae, and is good in quick-acting property and safe to crops.

Description

Technical Field

[0001] This invention belongs to the field of pesticide and insecticide technology, specifically relating to an insecticidal composition and its application. Background Technology

[0002] The problem of agricultural pest resistance is a global issue. With the long history of chemical control of agricultural pests and the influence of factors such as unscientific use of pesticides, agricultural pest resistance has become increasingly serious in recent years, and the number of resistant pests is continuously increasing. In particular, lepidopteran pests, which are very prone to developing resistance, have a high degree of resistance and the rate of resistance development is relatively fast.

[0003] Metaflumizone is a voltage-dependent sodium channel blocker that acts on the insect nervous system, blocking sodium channels and causing the insect nervous system to shut down and become paralyzed. Sodium channels participate in the conduction of action potentials along nerve axons. It has insecticidal activity itself, does not require bioactivation, and has no cross-resistance with existing insecticides.

[0004] Indoxacarb, a broad-spectrum insecticide belonging to the oxadiazine class, is a novel, green pesticide that primarily targets sodium channels and exhibits multiple effects on neuronal nAchRs and GABA receptors. It is characterized by high efficiency, high selectivity, and low residue. It shows no cross-resistance with other insecticides and is effective in controlling various pests on crops such as grains, cotton, fruits, and vegetables.

[0005] Pesticide compounding or mixing can broaden the insecticidal spectrum and delay the development of pesticide resistance in pests, making it one of the effective methods to address pest resistance issues. Therefore, researching and developing highly efficient, low-toxicity, and environmentally friendly insecticidal compositions is of positive significance for sustainable agricultural development. Furthermore, there are currently no reports or applications of mixing compounds of formula (Ⅰ) with cyfluthrin and indoxacarb for the control of lepidopteran pests. Summary of the Invention

[0006] The purpose of this invention is to provide an insecticidal composition whose activity is significantly higher than the sum of the activities of its individual active ingredients. It exhibits an unexpected, genuine synergistic effect, rather than simply the sum of its effects.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: an insecticidal composition comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I). The active ingredient B is either cyfluthrin or indoxacarb;

[0008] Furthermore, the mass ratio between active ingredient A and active ingredient B is 1:54 to 43:1.

[0009] Furthermore, the mass ratio of the compound of formula (I) to cyanflufenicol is 1:54 to 40:1, such as 1:54, 1:35, 1:32, 1:24, 1:20, 1:10, 1:8, 1:6, 1:5, 2:1, 8:1, 10:1, 22:1, 24:1, 40:1 or any value between the above values;

[0010] Furthermore, the mass ratio of the compound of formula (I) to cyanflufenicol is 1:35 to 24:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to cyanflufenicol is 1:35, 1:32, 1:24, 1:20, 1:10, 1:8, 1:6, 1:5, 2:1, 8:1, 10:1, 22:1, 24:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to indoxacarb is 1:30 to 43:1, such as 1:30, 1:20, 1:18, 1:14, 1:5, 7:4, 7:3, 4:1, 5:1, 8:1, 12:1, 16:1, 23:1, 43:1 or any value between the above values;

[0013] Furthermore, the mass ratio of the compound of formula (I) to indoxacarb is 1:20 to 23:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to indoxacarb is 1:20, 1:18, 1:14, 1:5, 7:4, 7:3, 4:1, 5:1, 8:1, 12:1, 16:1, or 23:1;

[0015] Furthermore, based on a total mass of 100 wt% of the insecticidal composition, the sum of the contents of active ingredient A and active ingredient B in the insecticidal composition is 0.5% to 90%.

[0016] Furthermore, based on a total mass of 100 wt% of the insecticidal composition, the sum of the contents of active ingredient A and active ingredient B in the insecticidal composition is 1% to 85%.

[0017] Furthermore, based on a total mass of 100 wt% of the insecticidal composition, the sum of the contents of active ingredient A and active ingredient B in the insecticidal composition is 5% to 70%.

[0018] Furthermore, the insecticidal composition further includes an adjuvant, which includes a carrier and an adjuvant.

[0019] Furthermore, the carrier is one or more of water, solvent, or filler;

[0020] Furthermore, the carrier is deionized water;

[0021] Further, the solvent is selected from one or more of the following: N,N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate, or acetonitrile.

[0022] Furthermore, the filler is selected from one or more of the following: kaolin, diatomaceous earth, bentonite, attapulgite, silica, starch, or light calcium carbonate.

[0023] The additives include at least one surfactant, and may also include other functional additives such as antifreeze, thickener, stabilizer, disintegrant, defoamer, slow-release agent, and binder, depending on the application and requirements.

[0024] The surfactant is selected from one or more of emulsifiers, dispersants, wetting agents, dispersion media, or penetrants;

[0025] The surfactants mentioned above are common nonionic or anionic surfactants, either as single agents or in combination; the other functional additives are selected from one or more of antifreeze agents, thickeners, stabilizers, disintegrants, or defoamers.

[0026] Furthermore, the emulsifier is selected from one or a mixture of multiple of the following: agricultural emulsion 500# (calcium alkylbenzene sulfonate), OP series phosphate esters (surfactant phosphate esters), 600# phosphate esters (phenylphenol polyoxyethylene ether phosphate esters), styrene polyoxyethylene ether wetting salts, alkyl biphenyl ether magnesium disulfonate salts, triethanolamine salts, agricultural emulsion 400# (benzyl dimethylphenol polyoxyethylene ether), agricultural emulsion 700# (alkylphenol formaldehyde resin polyoxyethylene ether), Ningxia emulsion 36# (phenylethylphenol formaldehyde resin polyoxyethylene ether), agricultural emulsion 1600# (phenylethylphenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymers, OP series (surfactants), BY series (castor oil polyoxyethylene ether), agricultural emulsion 33# (alkyl aryl polyoxyethylene polyoxypropylene ether), Span series (sorbitan monostearate), Tween series (dehydrated sorbitan fatty acid ester polyoxyethylene ether), or AEO series (fatty alcohol polyoxyethylene ether).

[0027] Furthermore, the dispersant is selected from one or more of the following: polycarboxylate, lignin sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, calcium alkylbenzene sulfonate, sodium naphthalene sulfonate formaldehyde condensate, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ether, or glycerol fatty acid ester polyoxyethylene ether.

[0028] Furthermore, the wetting agent is selected from one or more of the following: sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, pull-opening powder BX, wetting and penetrating agent F, soapberry powder, silkworm excrement, or soapberry powder;

[0029] Furthermore, the penetrant is selected from one or more of the following: penetrant JFC (fatty alcohol polyoxyethylene ether), penetrant T (diisooctyl maleate sulfonate), azone, or organosilicon;

[0030] Furthermore, the dispersion medium may be one or more of the following: soybean oil, rapeseed oil, wheat oil, methyl oleate, diesel oil, engine oil, and mineral oil.

[0031] Furthermore, the antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, or urea, or a mixture thereof;

[0032] Furthermore, the thickener is selected from one or more of xanthan gum, disintegrant, bentonite, carboxymethyl cellulose, or magnesium aluminum silicate;

[0033] Furthermore, the stabilizer is selected from one or more of the following: epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0034] Furthermore, the disintegrant is selected from one or more of the following: bentonite, urea, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid, or sodium bicarbonate;

[0035] Furthermore, the defoamer is selected from silicone oil, silicone compounds, and C. 10 ~C 20 Saturated fatty acid compounds or C8-C 10 A mixture of one or more fatty alcohol compounds.

[0036] Furthermore, the insecticidal composition can be formulated into pesticide-acceptable formulations, including solid formulations, liquid formulations, seed treatments, or other formulations;

[0037] Furthermore, the formulation dosage form is an emulsifiable concentrate, suspension concentrate, water-dispersible granules, water emulsion, microemulsion, granules, microcapsule suspension, ultra-low volume liquid, wettable powder, or dispersible oil suspension.

[0038] The application of an insecticidal composition in the control of agricultural and forestry pests, horticultural pests or sanitary pests;

[0039] Furthermore, the aforementioned agricultural and forestry pests, horticultural pests, and sanitary pests are pests belonging to the orders Hemiptera, Thysanoptera, Lepidoptera, and Diptera.

[0040] Furthermore, the aforementioned Hemiptera pests include the green stink bug (Acrosternum hilare), the corn stink bug (Blissus leucopterus), the black-spotted tobacco stink bug (Cyrtopeltis notatus), the cotton red stink bug (Dysdercus cingulatus), the intermedius stink bug (Dysdercus intermedius), the wheat flat shield stink bug (Eurygaster integriceps), the tobacco stink bug (Euschistus impictiventris), the cotton red boll-beaked stink bug (Leptoglossus phyllopus), the American pasture stink bug (Lygus lineolaris), the pasture stink bug (Lygus pratensis), the rice green stink bug (Nezara viridula), the beet lace stink bug (Piesma quadrata), the Solubea insularis, the Thyanta perditor, the Acyrthosiphononobrychis, and the larch ball aphid (Adelges). Aphidula nasturtii, beet aphid (Aphis fabae), strawberry root aphid (Aphis forbesi), apple aphid (Aphis pomi), cotton aphid (Aphis gossypii), North American tea aphid (Aphis grossulariae), schneideri, leaf roller aphid (Aphis spiraecola), elder aphid (Aphis sambuci), pea aphid (Acyrthosiphon pisum), eggplant webless aphid (Aulacorthum solani), silver leaf aphid (Bemisia argentif olii), short-tailed plum aphid (Brachycaudus cardui), short-tailed plum aphid (Brachycaudus helichrysi), short-tailed peach aphid (Brachycaudus persicae), short-tailed peach aphid (Brachycaudus prunicola), cabbage aphid (Brevicoryne brassicae), capitophorus horni, cerosipha gossypii, Chaetosiphon fragaefolii, Cryptomyzus ribis, Dreyfusia nordmannianae, Dreyfusia piceae, Dysaphisradicola), Dysaulacorthumpseudosolani, Dysaphis plantaginea, Dysaphis pyri, Empoasca fabae, Hyalopterus pruni, Hyperomyzus lactucae, Macrosiphum avenae, Macrosiphum euphorbiae, Macrosiphon rosae, Megoura viciae, Melanaphis pyrarius, Metopolophium dirhodum, Myzodes persicae, Myzus ascalonicus, Myzuscerasi, Myzus varians, Nasonovia ribis-nigri, Nilaparvata Lugens, Pemphigus bursarius, Perkinsiella saccharicida, Phorodon humuli, Psylla mali, Psyllapiri, Rhopalomyzus ascalonicus, Rhopalosiphum maidis, Rhopalosiphum padi, Rhopalosiphum inertum, Sappaphis mala, Sappaphis mali, Schizaphis graminum, Schizoneura lanuginosa, Sitobion avenae, Trialeurodes vaporariorum, Toxoptera aurantiiand, Viteus vitifolii, Cimex lectularius, Cimex tumefaciens hemipterus), Reduvius senilis, Triatoma and Arilus critatus;

[0041] Furthermore, the aforementioned Thysanoptera pests include orchid thrips (Dichromothripscorbetti), species of the genus Dichromothrips, brown thrips (Frankliniella fusca), alfalfa thrips (Frankliniella occidentalis), oriental thrips (Frankliniella tritici), bellflower thrips (Scirtothrips citri), rice thrips (Thrips oryzae), palm thrips (Thrips palmi), and tobacco thrips (Thrips tabaci).

[0042] Furthermore, the lepidopteran pests include: small cutworm (Agrotis ypsilon), yellow cutworm (Agrotissegetum), cotton beetle (Alabama argillacea), bean leafroller (Anticarsia gemmatalis), Argyresthia conjugella, forked leafroller (Autographa gamma), tree looper (Bupalus piniarius), Cacoecia murinana, Capua reticulana, Cheimatobia brumata, spruce leafroller (Choristoneura fumiferana), Choristoneura occidentalis, rice stem borer (Cirphis unipuncta), apple leafroller (Cydia pomonella), pine caterpillar (Dendrolimus pini), Diaphanianitidalis, southwestern corn stalk borer (Diatraea grandiosella), Egyptian leafminer (Earias insulana), and South American corn seedling borer (Elasmopalpus). lignosellus), privet leafroller (Eupoecilia ambiguella), evetria bouliana, feltia subterranea, wax moth (Galleria mellonella), plum fruit moth (Grapholitha funebrana), pear fruit moth (Grapholitha molesta), cotton bollworm (Heliothis armigera), tobacco shoot moth (Heliothis virescens), corn ear borer (Heliothis zea), cabbage moth (Hellulaundalis), hibernia defoliaria, fall webworm (Hyphantria cunea), apple leafminer (Hyponomeutamalinellus), tomato leafminer (Keiferia lycopersicella), lambdina fiscellaria, beet armyworm (Laphygma exigua), coffee leafminer (Leucoptera coffeella), spiral leafminer (Leucopterascitella), Lithocolletis blancardella, grape berry leafroller (Lobesia) botrana, beet web borer (Loxostege sticticalis), gypsy moth (Lymantria)The following are listed: * *dispar*, * *Lymantria monacha*, * *Lyonetia clerkella*, * *Malacosoma neustria*, * *Mamestrabrassicae*, * *Orgyia pseudotsugata*, * *Ostrinia nubilalis*, * *Panolis flammea*, * *Pectinophora gossypiella*, * *Peridromasaucia*, * *Phalera bucephala*, * *Phthorimaea operculella*, * *Phyllocnistis citrella*, * *Pieris brassicae*, * *Plathypena scabra*, * *Plutella xylostella*, * *Pseud oplusia includens*, * *Rhyacionia frustrana*, * *Scrobipalpula*. absoluta, wheat moth (Sitotrogacerealella), grape leafroller (Sparganothis pilleriana), meadow noctuid moth (Spodopterafrugiperda), sea gray-winged noctuid moth (Spodoptera littoralis), beet armyworm (Spodoptera litura), thaumatopoea pityocampa, green oak moth (Tortrix viridana), powdery noctuid moth (Trichoplusiani), and Zeiraphera canadensis;

[0043] Furthermore, the aforementioned Diptera include *Aedes aegypti*, *Aedes albopictus*, *Aedes vexans*, *Anastrepha ludens*, *Anopheles maculipennis*, *Anopheles crucians*, *Anopheles salbimanus*, *Anopheles gambiae*, *Anopheles freeborni*, *Anopheles leucosphyrus*, *Anopheles miimus*, *Bradysia odoriphaga Yang et Zhang*, *Anopheles quadrimaculatus*, *Calliphora vicina*, *Ceratitis capitata*, *Chrysomyabezziana*, *Chrysomya hominivorax*, and *Chrysomya*. *Chrysops discalis*, *Chrysops silacea*, *Chrysops atlanticus*, *Cochliomyia hominii vorax*, *Contarinia sorghicola*, *Cordylobia anthropophaga*, *Culicoides furens*, *Culex pipiens*, *Culex nigripalpus*, *Culex quinquefasciatus*, *Culex tarsalis*, *Culiseta inornata*, *Culisetamelanura*, *Dacus cucurbitae*, *Dacus oleae*, *Dasineura brassicae*, *Delia antique*, *Delia coarctata*, *Delia platura*, *Delia caesarea* radicum), Dermatobia hominis, Fanniacanicularis, Geomyza Tripunctata, Gasterophilus*Glossina intestinalis*, *Glossina morsitans*, *Glossina palpalis*, *Glossina fuscipes*, *Glossina tachinoides*, *Haematobia irritans*, *Haplodiplosis equestris*, *Hippelates spp.*, *Hylemyia platura*, *Hypoderma lineata*, *Leptoconops torrens*, *Liriomyza sativae*, *Liriomyza trifolii*, *Lucilia caprina*, *Lucilia cuprina*, *Lucilia sericata*, *Lycoria pectoralis*, *Mansonia titillanus*, *Mayetiola destructor*, *Musca autumnalis*, *Musca domestica*, *Muscina stabulans*, *Oestrus* *Ovis*, *Opomyza florum*, *Oscinella frit*, *Pegomyahysocyami*, *Phorbia antiqua*, *Phorbia brassicae*, *Phorbiacoarctata*, *Phlebotomus argentipes*, *Psorophora columbiae*, *Psila rosae*, *Psorophora discolor*, *Prosimulium mixtum*, *Rhagoletis cerasi*, *Rhagoletis pomonella*, *Sarcophaga haemorrhoidalis*, *Sarcophaga spp.*, *Simulium vittatum*, *Stomoxys calcitrans*, *Tabanus bovinus*, *Tabanus atratus*, *Tabanus lineola*, *Tabanus similis*, *Tipula* Oleracea and Tipula paludosa;

[0044] Furthermore, the lepidopteran pests mentioned are the beet armyworm and the diamondback moth;

[0045] Furthermore, the insecticidal composition is applied in an effective dose to the pest that needs to be controlled or to the medium in which it grows.

[0046] The insecticidal composition of the present invention has the following advantages:

[0047] The insecticidal composition of the present invention exhibits a synergistic effect within a certain ratio range, is highly effective against agricultural and forestry pests, significantly reduces pesticide usage, lowers pesticide costs, and reduces environmental impact; the two active ingredients have different mechanisms of action, which can delay the development of pest resistance. Detailed Implementation

[0048] The present invention will be further described below with reference to the embodiments. The percentages in the embodiments are all weight percentages, but the present invention is not limited thereto.

[0049] The compositions of the present invention can be provided in formulation form. They can be formulated as suspensions, water-dispersible granules, wettable powders, etc., as needed. The content of the active ingredient in the compositions of the present invention depends on the application rate when used alone, as well as on the mixing ratio and the degree of synergistic effect. The optimal range of active ingredient content varies depending on the type of formulation of the composition.

[0050] Formulation preparation examples:

[0051] Example 1:

[0052] 22% Formula (I) Compound·Cyanoflavone Suspension (2:20)

[0053] Formula composition: 2% compound of formula (I), 20% cyanflufenican, 1.5% sodium lignosulfonate, 3% phenethylphenol polyether phosphate salt, 1.5% sodium polycarboxylate salt, 3.5% alkyl aryl polyoxyethylene polyoxypropylene ether, 1% magnesium aluminum silicate, 0.2% xanthan gum, 0.1% methylisothiazolinone, 5% ethylene glycol, 0.5% silicone defoamer, deionized water to make up the balance;

[0054] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0055] Example 2:

[0056] 70% Formula (I) Compound·Cyanoflavone Water Dispersible Granules (10:60)

[0057] Formula composition: 10% compound of formula (I), 60% cyanflufenican, 10% sodium lignosulfonate, 4% BX powder, 6% sodium polycarboxylate, 6% starch, kaolin to make up the balance;

[0058] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0059] Example 3:

[0060] 3% Formula (I) compound·Cyanoflavone water emulsion (0.6:2.4)

[0061] Formula composition: 0.6% compound of formula (I), 2.4% cyanflufenican, 6% EO-PO block copolymer, 25% cyclohexanone, 0.25% xanthan gum, 5% glycerin, 3% urea, 0.5% sodium benzoate, 0.2% silicone defoamer, deionized water to make up the balance;

[0062] Preparation method: According to the formula ratio, the surfactant, antifreeze and other ingredients are mixed with water to form an aqueous phase. Then, the active ingredient is dissolved in a solvent and added to the aqueous phase under stirring. After stirring evenly, the thickener and preservative are added and shearing is continued for 10 minutes. Finally, the defoamer is added and stirred evenly to form an O / W type water emulsion.

[0063] Example 4:

[0064] 48% Formula (I) Compound Indoxacarb Water Dispersible Granules (42:6)

[0065] Formula composition: 42% compound of formula (I), 6% indoxacarb, 5% sodium lignin sulfonate, 3% tea saponin, 4% styrene powder BX, 6% sodium polycarboxylate, 6% starch, kaolin to make up the balance;

[0066] Preparation method: Same as in Example 2.

[0067] Example 5:

[0068] 24% Formula (I) Compound·Indoxacarb Suspension Concentrate (20:4)

[0069] Formula composition: 20% compound of formula (I), 4% indoxacarb, 1.5% sodium lignosulfonate, 3% phenethylphenol polyether phosphate salt, 1.5% sodium polycarboxylate salt, 1.5% agricultural emulsion 600-I, 3.5% alkyl aryl polyoxyethylene polyoxypropylene ether, 1% magnesium aluminum silicate, 0.2% xanthan gum, 0.1% methylisothiazolinone, 5% glycerin, 0.5% organosilicon defoamer, deionized water to make up the balance;

[0070] Preparation method: Same as in Example 1.

[0071] Example 6:

[0072] 10% Formula (I) Compound·Indoxacarb EC (8:2)

[0073] Formula composition: 8% compound of formula (I), 2% indoxacarb, 6.5% isomeric alcohol polyoxyethylene ether, 1% calcium dodecylbenzenesulfonate, 10% fatty alcohol polyoxyethylene ether, 4% corn oil, 15% cyclohexanone, 15% DMF, 20% decylamide, methyl oleate to make up the balance;

[0074] Preparation method: First, add the active ingredient to the solvent and dissolve it completely. Then add the emulsifier and stir evenly to form a uniform and transparent oily liquid. Fill the container to prepare the emulsifiable concentrate of the composition of the present invention.

[0075] The following biological test examples are used to illustrate the present invention. However, the present invention is not limited to these examples, and the reagents and solvents required for the tests are all provided by the Group's R&D Center.

[0076] Indoor bioactivity assay

[0077] Determination of the synergistic toxicity of different formulations against beet armyworm

[0078] Experimental target: Beet armyworm. Beet armyworm larvae were collected from scallion fields in Pingdu, Qingdao, Shandong Province. They were artificially reared for more than three generations in an insect rearing room. Healthy and uniform third-instar larvae were used for the experiment.

[0079] Test references: "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides" Part 6: Insect Immersion Method NY / T1154.6-2006; Part 7: Determination of Combined Effects of Mixtures NY / T 1154.7-2006; Part 14: Leaf Immersion Method NY / T1154.14-2008;

[0080] Drug preparation: Prepare single-dose stock solutions separately, and design the ratio according to the purpose of mixing and drug activity. Prepare 5 series of mass concentrations for each single agent and each group of mixed solutions according to the equal ratio method.

[0081] Experimental treatment: Fresh cabbage slices were placed in the drug solution with tweezers and soaked for 10 seconds. After the solution was allowed to air dry, the cabbage slices were placed in a petri dish lined with moisturizing filter paper. Fifteen 3rd instar larvae of uniform development were placed in the drug solution for 5 seconds. Excess solution was absorbed with filter paper, and the test insects were placed in a petri dish with leaves soaked at the corresponding concentration.

[0082] Each treatment was repeated four times, with a blank treatment serving as a control. The treated insects were then reared in an AI-controlled culture room at 26±1℃, with a light duration of L:D = 16h:8h and a relative humidity of 60%.

[0083] Investigation method: The mortality of test insects was investigated 48 hours after the treatment with the agent. The criteria for judging the mortality of test insects were obvious shrinkage of the insect body or inability to crawl normally when punctured. The total number of insects and the number of dead insects were recorded.

[0084] Data statistics and analysis:

[0085] 48 hours after treatment, examine the mortality of the test insects and record the total number of insects and the number of dead insects. Calculate the corrected mortality rate for each treatment based on the survey data using the following formula:

[0086]

[0087] In the formula:

[0088] P – Mortality rate, expressed as a percentage (%);

[0089] K represents the number of dead insects, in heads;

[0090] N represents the total number of insects treated, in units of heads.

[0091]

[0092] In the formula:

[0093] P1 – Corrected mortality rate, in percentage (%);

[0094] P t —The mortality rate is expressed as a percentage (%).

[0095] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0096] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0097] The data is processed using probability value analysis. The DPS statistical analysis system can be used to analyze the data and determine the LC of the toxicity regression line. 50 The b-value, its 95% confidence limit, and correlation coefficient r are used to evaluate the activity of the test reagent on the biological material.

[0098] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0099]

[0100] In the formula:

[0101] ATI – Actual Measured Toxicity Index of Mixtures;

[0102] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0103] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0104] TTI = TI A *P A +TI B *P B

[0105] In the formula:

[0106] TTI – Theoretical Toxicity Index of Mixtures;

[0107] TI A —A. Toxicity index of drug A;

[0108] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0109] TI B —Toxicity index of drug B;

[0110] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0111]

[0112] In the formula:

[0113] CTC – Cotoxicity Coefficient;

[0114] ATI – Actual Measured Toxicity Index of Mixtures;

[0115] TTI – Theoretical Toxicity Index of Mixtures.

[0116] Co-toxicity coefficients of compound formulations: CTC≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; 80<CTC<120 indicates an additive effect.

[0117] Experimental results:

[0118] Table 1 shows the LC50 results of compound (Ⅰ) against beet armyworm. 50 It is 0.639 mg·L -1When compound (I) is mixed with cyfluthrin, the combined effect of compound (I) and cyfluthrin in the range of 1:54 to 40:1 in controlling beet armyworm is additive or synergistic. When compound (I) and cyfluthrin are mixed in the range of 1:54 to 22:1 in controlling beet armyworm, the co-toxicity coefficient is greater than 120, which is synergistic.

[0119] Table 1. Results of toxicity determination of compound (I) and different ratios of cyhalofop-P-ethyl to beet armyworm.

[0120]

[0121]

[0122] Table 2 shows that when compound (I) is mixed with indoxacarb, the combined effect in controlling beet armyworm is additive or synergistic in the range of 1:30 to 43:1; among them, compound (I) and indoxacarb show a synergistic effect in controlling beet armyworm in the range of 1:18 to 43:1; and the co-toxicity coefficient is greater than 150 in the range of 1:5 to 8:1, showing a significant synergistic effect.

[0123] Table 2 shows the toxicity test results of compound (I) and different ratios of indoxacarb against beet armyworm.

[0124]

[0125] Determination of the combined toxicity of different formulations on diamondback moth

[0126] Experimental target: Diamondback moth. Diamondback moth larvae were collected from Chinese cabbage in Pingdu, Qingdao, Shandong Province. They were artificially reared for more than 3 generations in an insect rearing room. Healthy and uniform 3rd instar larvae were used for the experiment.

[0127] Test references: "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides" Part 6: Insect Immersion Method NY / T1154.6-2006; Part 7: Determination of Combined Effects of Mixtures NY / T 1154.7-2006; Part 14: Leaf Immersion Method NY / T1154.14-2008;

[0128] Drug preparation: Prepare single-dose stock solutions separately, and design the ratio according to the purpose of mixing and drug activity. Prepare 5 series of mass concentrations for each single agent and each group of mixed solutions according to the equal ratio method.

[0129] Experimental treatment: Fresh cabbage slices were placed in the drug solution for 10 seconds with tweezers and then removed. After the drug solution was allowed to air dry naturally, the cabbage slices were placed in a petri dish lined with moisturizing filter paper. Twenty 3rd instar larvae of uniform development were placed in the drug solution for 5 seconds. Excess drug solution was absorbed with filter paper, and the test insects were placed in a petri dish with leaves soaked at the corresponding concentration.

[0130] Each treatment was repeated four times, with a blank treatment serving as a control. The treated insects were then reared in an AI-controlled culture room at 26±1℃, with a light duration of L:D = 16h:8h and a relative humidity of 60%.

[0131] Investigation method: The mortality of test insects was investigated 48 hours after the treatment with the agent. The criteria for judging the mortality of test insects were obvious shrinkage of the insect body or inability to crawl normally when punctured. The total number of insects and the number of dead insects were recorded.

[0132] Data statistics and analysis:

[0133] 48 hours after treatment, examine the mortality of the test insects and record the total number of insects and the number of dead insects. Calculate the corrected mortality rate for each treatment based on the survey data using the following formula:

[0134]

[0135] In the formula:

[0136] P – Mortality rate, expressed as a percentage (%);

[0137] K represents the number of dead insects, in heads;

[0138] N represents the total number of insects treated, in units of heads.

[0139]

[0140] In the formula:

[0141] P1 – Corrected mortality rate, in percentage (%);

[0142] P t —The mortality rate is expressed as a percentage (%).

[0143] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0144] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0145] The data is processed using probability value analysis. The DPS statistical analysis system can be used to analyze the data and determine the LC of the toxicity regression line. 50 The b-value, its 95% confidence limit, and correlation coefficient r are used to evaluate the activity of the test reagent on the biological material.

[0146] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0147]

[0148] In the formula:

[0149] ATI – Actual Measured Toxicity Index of Mixtures;

[0150] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0151] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0152] TTI = TI A *P A +TI B *P B

[0153] In the formula:

[0154] TTI – Theoretical Toxicity Index of Mixtures;

[0155] TI A —A. Toxicity index of drug A;

[0156] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0157] TI B —Toxicity index of drug B;

[0158] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0159]

[0160] In the formula:

[0161] CTC – Cotoxicity Coefficient;

[0162] ATI – Actual Measured Toxicity Index of Mixtures;

[0163] TTI – Theoretical Toxicity Index of Mixtures.

[0164] Co-toxicity coefficients of compound formulations: CTC≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; 80<CTC<120 indicates an additive effect.

[0165] Experimental results:

[0166] Table 3 shows the LC50 results of compound (I) against diamondback moth. 50 It is 0.038 mg·L -1 LC50 of cyfluthrin against diamondback moth 50 It is 30.871 mg·L -1When compound (I) is mixed with cyfluthrin, it exhibits a synergistic effect in controlling diamondback moth within a ratio of 1:35 to 24:1. When compound (I) is mixed with cyfluthrin, the co-toxicity coefficient of the synergistic effect in controlling diamondback moth within a ratio of 1:35 to 8:1 is greater than 130, indicating a significant synergistic effect.

[0167] Table 3 shows the toxicity test results of compound (I) and different ratios of cyhalofop-Pyrantel hydrazone on diamondback moth.

[0168]

[0169]

[0170] Table 4 shows that when compound (I) is mixed with indoxacarb, the combined effect in controlling diamondback moth is synergistic in the range of 1:20 to 23:1; among them, compound (I) and indoxacarb show a significant synergistic effect in controlling diamondback moth in the range of 1:14 to 5:1.

[0171] Table 4 shows the toxicity test results of compound (I) and indoxacarb in different ratios against diamondback moth.

[0172]

[0173] Field efficacy examples

[0174] Efficacy test of different pesticides in controlling beet armyworm in cabbage fields

[0175] Refer to GB / T 17980.13—2000 "Guidelines for Field Efficacy Testing of Pesticides (I) Part 13 Insecticides for the Control of Lepidoptera Larvae in Cruciferous Vegetables".

[0176] Trial time and location: September 2022, Pingdu cabbage planting base, Qingdao City, Shandong Province;

[0177] Test crop: Cabbage (Jingfeng No. 1), cabbage in the early rosette stage;

[0178] Target pest: Beet armyworm;

[0179] Cell block setup: Each treatment cell block has an area of ​​20m². 2 Four replicates were set up, and the cultivation conditions of all experimental plots were uniform.

[0180] Test method: spray method;

[0181] Weather: The weather was sunny on the day of application, with temperatures ranging from 18°C ​​to 27°C; no adverse weather conditions that could have affected the results occurred during the entire experiment.

[0182] Investigation: The initial insect population was investigated before pesticide application, and the number of live insects was investigated 3 days and 7 days after pesticide application. Ten plants were selected from each plot for fixed-point investigation of the number of larvae of each instar.

[0183] Application of pesticides:

[0184] Table 5. Drug treatment and dosage

[0185]

[0186] Methods for calculating drug efficacy:

[0187]

[0188] Data analysis: Duncan's new multiple range method was used to analyze the significance of differences (P<0.05 indicates significant difference).

[0189] Experimental results:

[0190] Field efficacy trials showed that, as shown in Table 6, the combination of compound (I) with cyfluthrin and indoxacarb had good control effects against beet armyworm. Three days after application, the control efficacy of 22% compound (I)·cyfluthrin suspension (2:20) and 24% compound (I)·indoxacarb suspension (20:4) against beet armyworm was 82.56% and 80.39%, respectively; seven days after application, the control efficacy of 22% compound (I)·cyfluthrin suspension (2:20) and 24% compound (I)·indoxacarb suspension (20:4) against beet armyworm was 91.91% and 90.1%, respectively.

[0191] Table 6. Field efficacy trials of different treatments against beet armyworm.

[0192]

[0193]

[0194] Note: All data above are the average of 4 repetitions, with values ​​rounded to two decimal places. Letters indicate significance (0.05).

[0195] Efficacy tests of different pesticides for controlling diamondback moth

[0196] Refer to GB / T 17980.13—2000 "Guidelines for Field Efficacy Testing of Pesticides (I) Part 13 Insecticides for the Control of Lepidoptera Larvae in Cruciferous Vegetables".

[0197] Experiment time and location: August 2022, Jimo cabbage planting base in Qingdao City, Shandong Province;

[0198] Test crop: Chinese cabbage, in the early rosette stage;

[0199] Target pest: Diamondback moth;

[0200] Cell block setup: Each treatment cell block has an area of ​​20m². 2 Four replicates were set up, and the cultivation conditions of all experimental plots were uniform.

[0201] Test method: spray method;

[0202] Weather: The weather was sunny on the day of application, with temperatures ranging from 22°C to 32°C; no adverse weather conditions that could have affected the test results occurred during the entire experiment.

[0203] Investigation: The initial insect population was investigated before pesticide application, and the number of live insects was investigated 3 days and 7 days after pesticide application. Ten plants were selected from each plot for fixed-point investigation of the number of larvae of each instar.

[0204] Application of pesticides:

[0205] Table 7. Drug Treatment and Dosage

[0206]

[0207] Methods for calculating drug efficacy:

[0208]

[0209] Data analysis: Duncan's new multiple range method was used to analyze the significance of differences (P<0.05 indicates significant difference).

[0210] Experimental results:

[0211] Field efficacy trials showed that, as shown in Table 8, the mixture of compound (I) with cyfluthrin and indoxacarb had good control effects on diamondback moth. Three days after application, the control efficacy of 22% compound (I)·cyfluthrin suspension (2:20) and 24% compound (I)·indoxacarb suspension (20:4) against diamondback moth was 84.50% and 82.96%, respectively; seven days after application, the control efficacy of 22% compound (I)·cyfluthrin suspension (2:20) and 24% compound (I)·indoxacarb suspension (20:4) against diamondback moth was 93.63% and 90.36%, respectively.

[0212] Table 8. Field control efficacy of each treatment against diamondback moth.

[0213]

[0214] Note: All data above are averages of four replicates, with values ​​rounded to two decimal places. Letters indicate significance (0.05).

[0215] The insecticidal composition or formulation obtained by this invention exhibits significant control efficacy, superior to single-agent formulations in delaying the development of resistance and prolonging pesticide retention. Furthermore, no phytotoxicity was observed in the experiments with the compounded formulation, indicating that the improved synergistic effect of the obtained insecticidal composition or formulation can reduce production and usage costs while ensuring crop safety.

[0216] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it based on the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. An insecticidal composition, characterized in that: It includes active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I). The active ingredient B is either cyfluthrin or indoxacarb.

2. The insecticidal composition according to claim 1, characterized in that: The mass ratio of active ingredient A to active ingredient B is 1:54 to 43:

1.

3. The insecticidal composition according to claim 1, characterized in that: The mass ratio of the compound of formula (I) to cyfluthrin is 1:54 to 40:1; the mass ratio of the compound of formula (I) to indoxacarb is 1:30 to 43:

1. Preferably, the mass ratio of the compound of formula (I) to cyfluthrin is 1:35 to 24:1; and the mass ratio of the compound of formula (I) to indoxacarb is 1:20 to 23:

1.

4. The insecticidal composition according to claim 1, characterized in that: Based on a total mass of 100 wt% of the insecticidal composition, the sum of the contents of active ingredient A and active ingredient B in the insecticidal composition is 0.5% to 90%. Preferably, the sum of the contents of active ingredient A and active ingredient B in the insecticidal composition is 1% to 85%.

5. The insecticidal composition according to claim 1, characterized in that: The insecticidal composition further includes adjuvants, which include carriers and adjuvants.

6. The insecticidal composition according to claim 1, characterized in that: The insecticidal composition can be formulated into pesticide-acceptable formulations, including solid and liquid formulations.

7. The insecticidal composition according to claim 6, characterized in that: The formulation is an emulsifiable concentrate, suspension concentrate, water-dispersible granule, water emulsion, microemulsion, granule, microcapsule suspension, ultra-low volume liquid, wettable powder, or dispersible oil suspension.

8. The use of the insecticidal composition according to any one of claims 1-7 in the control of agricultural and forestry pests, horticultural pests or sanitary pests.

9. The application according to claim 8, characterized in that: The pests mentioned are Hemiptera, Thysanoptera, Lepidoptera, and Diptera pests; Preferably, the lepidopteran pests are beet armyworm and diamondback moth.

10. The application according to claim 9, characterized in that: The insecticidal composition is applied in an effective dose to the pest that needs to be controlled or to the medium in which it grows.

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