Cosmetic composition for improving skin condition comprising nicotinamide mononucleotide (NMN)

Abstract

The present invention relates to a cosmetic composition for improving the skin condition comprising nicotinamide mononucleotide (NMN). Specifically, the present invention provides a composition comprising a sponge microneedle, a lactic acid bacteria exosome, and ceramide in a weight ratio of 0.6: 3.0: 0.01, the lactic acid bacteria exosome being obtained by culturing a Lactobacillus curvatus GFC-AM5 strain (Concentration Number: KCCM12245P) at 37 DEG C for 20 hours and then concentrating the strain by 40 times or more, and the ceramide being a mixture of the sponge microneedle, the lactic acid bacteria exosome, and the ceramide. Wherein the nicotinamide mononucleotide (NMN) is contained in an amount of 0.001 to 3% by weight relative to the total weight of the composition. The sponge microneedle promotes transmission of effective components by forming a microchannel on the skin surface, the exosome provides a cell signal transduction function, the NMN promotes cell energy metabolism by activating an NADbiosynthetic pathway in skin cells, so that cell proliferation and regeneration are enhanced, and the ceramide provides a function of strengthening a skin barrier. Accordingly, the composition of the present invention can simultaneously achieve the effects of improving skin barrier, activating cells and promoting wound healing, and can be applied to various cosmetic formulations.

Description

TECHNICAL FIELD

[0001] The present application relates to a cosmetic composition for improving skin condition comprising nicotinamide mononucleotide (NMN). BACKGROUND

[0002] Skin is a biological barrier that protects the human body from the external environment, and maintains homeostasis through structural stability centered on the stratum corneum and cell regeneration capacity. However, due to ultraviolet rays, fine particulate matter, oxidative stress, and metabolic function decline with aging, the skin barrier function can be weakened, and thus problems such as increased water loss, decreased elasticity, wrinkle formation, and decreased regenerative capacity can occur. Accordingly, various functional cosmetic compositions for improving skin barrier and promoting cell activity are being continuously developed.

[0003] Recently, a technology of forming microchannels on the skin using spicules to promote the delivery of active ingredients has been proposed, and exosomes derived from lactic acid bacteria are attracting attention as ingredients that promote skin cell activation and regeneration through intercellular signaling. In addition, ceramide, which is a major lipid component of the stratum corneum, plays an important role in strengthening the skin barrier and maintaining moisture. A composition containing these ingredients and having a specific weight ratio can exhibit a skin condition improvement effect.

[0004] On the other hand, nicotinamide mononucleotide (NMN) is attracting attention in terms of cellular energy metabolism and aging regulation. NMN plays a role as a precursor of NAD⁺ in the body, activates intracellular energy metabolism, and is known to be involved in the activation of SIRT series proteins. The NAD⁺ level of skin cells has a tendency to decrease with aging, and as a result, cell regeneration capacity and damage repair capacity also decrease. In this regard, NMN has been proposed as a possibility for application as an ingredient related to improving skin aging and promoting cell activity.

[0005] However, in the prior art, sufficient technical consideration has not been made regarding the interaction or synergistic effect when using a skin improvement composition containing spicule, lactic acid bacteria exosome, and ceramide together with NMN that activates cellular energy metabolism. In particular, a technical approach in which NMN is simultaneously delivered to skin cells with exosomes through the microchannels formed by spicules to simultaneously induce cell signaling and energy metabolism activation has not been clearly proposed.

[0006] Therefore, there is a need for a composition that can provide a further improved skin condition improvement effect by additionally providing a cell energy metabolism activation effect while maintaining skin barrier improvement function and cell regeneration signaling function. SUMMARY

[0007] The present application is directed to a skin condition-improving composition comprising a sponge microneedle, a lactic acid bacteria exosome, and a ceramide in a specific weight ratio, and aims to provide a cosmetic composition that provides a further improved skin improvement effect by activating cell energy metabolism while maintaining skin barrier strengthening and cell signaling function promotion.

[0008] Specifically, although the existing skin improvement composition can exhibit skin barrier restoration and cell activation effects, it can have limitations in sufficiently restoring the cell functions that decrease due to aging because it does not include a component that directly increases the intracellular energy metabolism level itself. In particular, the NAD⁺ level of skin cells has a tendency to decrease due to aging and oxidative stress, and as a result, the cell regeneration ability and damage repair ability also decrease.

[0009] Accordingly, the present application aims to provide a cosmetic composition for improving skin condition that activates the intracellular NAD⁺ biosynthesis pathway by including a specific content range of nicotinamide mononucleotide (NMN) and effectively delivers NMN to skin cells together with a lactic acid bacteria exosome through a microchannel formed by a sponge microneedle, thereby simultaneously achieving cell signaling function and energy metabolism activation function.

[0010] In addition, another object of the present application is to provide a composition that is improved in cell proliferation, wound healing, and skin condition improvement effects compared to a composition not containing NMN.

[0011] The cosmetic composition for improving skin condition according to an embodiment of the present application includes a core part and a shell layer surrounding the core part, wherein the sponge microneedle: lactic acid bacteria exosome: ceramide is included in a weight ratio of 0.6:3.0:0.01, and the lactic acid bacteria exosome is obtained by culturing Lactobacillus curvatus GFC-AM5 strain (Deposition Number: KCCM12245P) at 37°C for 20 hours and then concentrating it by 40 times or more, and the content of the nicotinamide mononucleotide is 0.001 to 3% by weight with respect to the total weight of the cosmetic composition.

[0012] The content of the nicotinamide mononucleotide is 0.01 to 1% by weight, and the composition activates the NAD⁺ biosynthesis pathway of skin cells through the nicotinamide mononucleotide.

[0013] In addition, the composition is characterized in that the skin cell proliferation or wound healing effect is improved compared to a composition not containing the nicotinamide mononucleotide, and the composition is characterized in that it is formed in a cream, emulsion, lotion, serum, ampoule, or facial mask patch type.

[0014] The cosmetic composition for improving skin condition according to the present invention has the following effects: First, by including nicotinamide mononucleotide (NMN), it can activate the NAD⁺ biosynthetic pathway in skin cells, thereby enhancing cellular energy metabolism and improving cell function. Second, through the microchannels formed by the sponge microneedles, lactic acid bacteria exosomes and NMN can be more effectively delivered to skin cells. The cell signaling function of exosomes and the energy metabolism activation function of NMN complement each other, enhancing cell proliferation and regeneration. Third, by strengthening the skin barrier and improving moisture retention capacity through ceramides, an environment is created that allows the energy metabolism activation effect to be stably expressed. Fourth, compared with compositions without NMN, the composition according to the present invention can increase cell proliferation rate and wound healing speed, thereby comprehensively improving the skin condition. Therefore, the present invention, through multiple mechanisms of action including improving the skin barrier, promoting cell signaling, and activating cellular energy metabolism, can provide a further improved skin improvement effect compared to existing compositions. Attached Figure Description

[0015] Figure 1 This is a diagram that briefly illustrates the mechanism of action of a cosmetic composition containing nicotinamide mononucleotide (NMN) for improving skin condition according to an embodiment of the present invention.

[0016] Figure 2 This is a graph showing the changes in intracellular NAD⁺ levels after skin cells were treated with compositions containing different NMN contents prepared according to embodiments of the present invention.

[0017] Figure 3 The graphs compare the intracellular NAD⁺ levels after treating skin keratinocytes (HaCaT) with the skin-improving cosmetic composition containing NMN according to Example 1 of the present invention and the NMN-free composition of Comparative Example 1.

[0018] Figure 4 The graphs compare the cell proliferation rates of skin fibroblasts after treating skin fibroblasts with the cosmetic composition containing NMN for improving skin condition according to Example 1 of the present invention and the composition without NMN in Comparative Example 1.

[0019] Figure 5 The graphs compare the wound closure rates over time after treating skin fibroblasts with the skin-improving cosmetic composition containing NMN according to Example 1 of the present invention and the composition without NMN according to Comparative Example 1. Detailed Implementation

[0020] Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art can readily implement the invention. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. For clarity of illustration, parts unrelated to the description have been omitted from the drawings, and throughout the specification, the same reference numerals are used for the same or similar constituent elements.

[0021] In this specification, terms such as "comprising" or "having" are intended to specify the presence of features, figures, steps, actions, constituent elements, parts, or combinations thereof described in the specification, and should not be construed as precluding the possibility of the presence or addition of one or more other features, figures, steps, actions, constituent elements, parts, or combinations thereof. Furthermore, when it is said that a portion such as a layer, film, region, plate, etc., is "above" another portion, this includes not only the case where it is "directly above" the other portion, but also the case where the other portion is present in between. Conversely, when it is said that a portion is "below" another portion, this includes not only the case where it is "directly below" the other portion, but also the case where the other portion is present in between.

[0022] Figure 1 This is a schematic diagram illustrating the mechanism of action of a cosmetic composition comprising nicotinamide mononucleotide (NMN) for improving skin condition according to an embodiment of the present invention. (Reference) Figure 1 The skin is composed of the stratum corneum, epidermis, and dermis. Microchannels can be formed in the stratum corneum using sponge microneedles applied to the skin surface. Through these microchannels, lactic acid bacteria exosomes, ceramides, and nicotinamide mononucleotide (NMN) can be delivered into the skin. The delivered NMN activates the NAD⁺ biosynthesis pathway within skin cells to promote cellular energy metabolism and works in conjunction with the cell signaling function of exosomes to enhance skin cell activity and regeneration. Furthermore, ceramides strengthen the skin barrier, allowing the effects of these active ingredients to be stably maintained.

[0023] The cosmetic composition for improving skin condition according to the present invention comprises sponge microneedles, lactic acid bacteria exosomes, ceramides, and nicotinamide mononucleotide (NMN). Preferably, the sponge microneedles, lactic acid bacteria exosomes, and ceramides are contained in a weight ratio of 0.6:3.0:0.01. This weight ratio is based on a combination ratio proven to have excellent skin barrier improvement and cell activation effects, aiming to balance the physiological activity of exosomes with the barrier-strengthening effect of ceramides. The lactic acid bacteria exosomes can be obtained by concentrating the culture medium of Lactobacillus curvatus GFCAM5 strain (accession number: KCCM12245P) at 37°C for 20 hours by more than 40 times. The exosomes are vesicles containing intercellular signaling substances, which can help promote the activation and regeneration of skin cells. The sponge microneedles can be of hydrolyzed sponge origin, which can promote the delivery of active ingredients by forming microchannels on the skin surface. In addition, the ceramide can be yeast-derived ceramide NP, which can provide functions of strengthening the skin barrier and maintaining moisture.

[0024] In this invention, the content of nicotinamide mononucleotide (NMN) is preferably 0.001 to 3% by weight relative to the total weight of the cosmetic composition, more specifically, it can be in the range of 0.01 to 1% by weight. If the content is below the lower limit (0.001% by weight), the increase in intracellular NAD⁺ levels is limited, resulting in the NMN-induced activation effect on cellular energy metabolism not being fully realized. Conversely, if the NMN content exceeds the upper limit (3% by weight), the increase in NAD⁺ levels will tend to plateau, further improvement in effect will be negligible, and it may become unfavorable in terms of dosage form stability or the possibility of skin irritation. Therefore, this invention ensures a balance between efficacy and dosage form suitability by limiting the NMN content to the range of 0.001 to 3% by weight. This range is designed to effectively activate the NAD⁺ biosynthetic pathway in skin cells while minimizing irritation or instability. NMN functions as a precursor to NAD⁺ in cells, activating energy metabolism and participating in the regulation of cellular functions related to the activity of the SIRT series of proteins.

[0025] Figure 2 This is a graph showing the changes in intracellular NAD⁺ levels after skin cells were treated with compositions containing different NMN contents prepared according to embodiments of the present invention. (Reference) Figure 2Within the NMN content range below 0.001 wt%, the increase in NAD⁺ levels is limited, while in the range of 0.001 to 3 wt%, NAD⁺ levels show a significant increasing trend. Furthermore, in the range above 3 wt%, the increase in NAD⁺ tends to saturate, showing a trend of limited further increase, which supports the critical significance of the NMN content range proposed in this invention.

[0026] In this invention, NMN, together with exosomes, can be delivered to skin cells through microchannels formed by sponge microneedles, thereby simultaneously achieving cell signal transduction and energy metabolism activation functions. After preparing a basic composition by uniformly mixing the sponge microneedles, lactic acid bacteria exosomes, and ceramides at the stated weight ratio, NMN is added to the composition at a predetermined content range and stirred uniformly to prepare the cosmetic composition according to the invention. During the preparation process, NMN can be added in the form of an aqueous phase or an ethanol-water solution, and the pH of the composition is preferably adjusted to the range of 5.0 to 6.5.

[0027] Example 1: Preparation of a composition containing NMN. A basic composition was prepared by mixing sponge microneedles, lactic acid bacteria exosomes, and ceramides in a weight ratio of 0.6:3.0:0.01. The lactic acid bacteria exosomes were obtained by concentrating Lactobacillus curvatus GFC-AM5 strain at 37°C for 20 hours at a concentration greater than 40 times. Subsequently, NMN was added to a content of 0.1% by weight relative to the total composition, and the mixture was stirred uniformly to prepare the composition of the present invention.

[0028] Comparative Example 1: An NMN-free composition was prepared by mixing sponge microneedles, lactic acid bacteria exosomes and ceramides in the same manner as in Example 1, but without adding NMN, and was used as the comparative group.

[0029] Experimental Example 1: Intracellular NAD⁺ Level Analysis. Intracellular NAD⁺ levels were measured after treating skin keratinocytes (HaCaT) with the compositions of Example 1 and Comparative Example 1. The results showed that the composition of Example 1, containing NMN, exhibited a trend of increased intracellular NAD⁺ levels compared to Comparative Example 1. Figure 3 This is a graph comparing the intracellular NAD⁺ levels after treating skin keratinocytes (HaCaT) with the NMN-containing cosmetic composition for improving skin condition according to Example 1 of the present invention and the NMN-free composition of Comparative Example 1. (Reference) Figure 3 Compared to the Comparative Example 1 treatment group without NMN, the NMN-containing treatment group (Example 1) showed a significant increase in intracellular NAD⁺ levels. This indicates that the composition of the present invention can promote cellular energy metabolism by activating the NAD⁺ biosynthetic pathway in skin cells through NMN.

[0030] Experimental Example 2: Evaluation of Cell Proliferation Effect. After treating skin fibroblasts with the compositions of Example 1 and Comparative Example 1, the cell proliferation rate was measured. The results confirmed that the cell proliferation rate of Example 1, which contained NMN, was improved compared with Comparative Example 1. Figure 4 This is a graph comparing the cell proliferation rates of skin fibroblasts treated with the NMN-containing cosmetic composition for improving skin condition according to Example 1 of the present invention and the NMN-free composition of Comparative Example 1. (Reference) Figure 4 Compared with the Comparative Example 1 treatment group (without NMN) and the untreated control group, the NMN-containing Example 1 treatment group showed a trend of increased cell proliferation rate. This indicates that the composition of the present invention can promote skin cell proliferation by activating the intracellular NAD⁺ biosynthesis pathway through NMN and interacting with the cell signaling function of exosomes.

[0031] Experimental Example 3: Evaluation of Wound Healing Effect. After creating scratches on skin fibroblasts, Examples 1 and Comparative Example 1 were treated, and the wound closure rate was measured. The results showed that Example 1, containing NMN, exhibited a higher wound healing rate than Comparative Example 1 within the same timeframe. Figure 5 This is a graph comparing the wound closure rate over time after treating skin fibroblasts with the NMN-containing cosmetic composition for improving skin condition according to Example 1 of the present invention and the NMN-free composition of Comparative Example 1. (Reference) Figure 5 Compared with the NMN-free Comparative Example 1 treatment group and the untreated control group, the NMN-containing Example 1 treatment group showed a higher wound closure rate within the same time period. This indicates that the cell energy metabolism activation achieved by NMN in the composition of the present invention and the cell signaling effect of exosomes complement each other, thereby promoting skin cell migration and proliferation, and thus improving wound healing speed.

[0032] The compositions according to the present invention can be prepared into various cosmetic dosage forms such as face creams, lotions, emulsions, serums, ampoules, or sheet masks. For example, the compositions of the present invention can be included in an emulsion dosage form containing an aqueous phase and an oil phase in a certain proportion, and a stable dosage form can be achieved by appropriately combining thickeners and moisturizers. When the dosage form prepared as described above is applied to the skin, the delivery of active ingredients is promoted by the sponge microneedles, and the signal transduction function of exosomes complements the NAD⁺ pathway activation function of NMN, which can further improve the skin condition.

[0033] The embodiments of the present invention have been described above, but the concept of the present invention is not limited to the embodiments presented in this specification. Those skilled in the art can easily propose other embodiments by adding, changing, deleting, or supplementing the constituent elements within the same conceptual scope, and these should also be considered to fall within the scope of the present invention.

Claims

1. A cosmetic composition comprising nicotinamide mononucleotide (NMN) for improving skin condition, characterized in that, include: The composition comprises microneedles, lactic acid bacteria exosomes, ceramide, and nicotinamide mononucleotide (MN); wherein the microneedles, lactic acid bacteria exosomes, and ceramide are contained in a weight ratio of 0.6:3.0:0.01; the lactic acid bacteria exosomes are obtained by culturing Lactobacillus curvatus GFC-AM5 strain (accession number: KCCM12245P) at 37°C for 20 hours and then concentrating it more than 40 times; the nicotinamide mononucleotide content relative to the total weight of the cosmetic composition is 0.001 to 3% by weight.

2. The cosmetic composition for improving skin condition comprising nicotinamide mononucleotide (NMN) as described in claim 1, characterized in that, The content of the nicotinamide mononucleotide is 0.01 to 1% by weight.

3. The cosmetic composition for improving skin condition comprising nicotinamide mononucleotide (NMN) as described in claim 1 or 2, characterized in that, The composition activates the NAD⁺ biosynthesis pathway in skin cells via the nicotinamide mononucleotide.

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