Marine unicellular microalgae propagation culture device

Abstract

The application belongs to the technical field of microalgae cultivation, and particularly relates to a marine single-cell microalgae propagation culture device, which comprises a culture tank, the inner wall of the culture tank is fixedly installed with an upper sealing plate, the inner wall of the upper sealing plate is uniformly fixedly installed with a plurality of air guide pipes, one end of each of the plurality of air guide pipes is fixedly installed with a one-way exhaust valve, the one-way exhaust valves are all arranged above the upper sealing plate, the inner wall of the culture tank is fixedly installed with a carbon dioxide gas supply pipe, the height of the carbon dioxide gas supply pipe is lower than the height of the upper sealing plate, and a sealing assembly is arranged below the upper sealing plate. Carbon dioxide is simultaneously sprayed out from each one-way exhaust valve, the design avoids the centralized release of carbon dioxide from one or a few fixed points, can make the carbon dioxide more evenly dispersed to each area of the culture tank, and effectively solves the problem of excessively high or low local carbon dioxide concentration possibly caused by a traditional gas supply mode.

Description

Technical Field

[0001] This invention belongs to the field of microalgae cultivation technology, specifically a marine single-cell microalgae propagation and cultivation device. Background Technology

[0002] In the food industry, marine single-celled microalgae are gradually emerging as a promising new food resource and additive source. Marine single-celled microalgae are rich in high-quality protein, various unsaturated fatty acids, vitamins, minerals, and bioactive substances such as DHA and EPA. These nutrients are of great importance in enhancing the nutritional value of food, improving food quality, and developing functional foods. With the increasing demand for natural, healthy, and functional raw materials in the food industry, the large-scale propagation and cultivation of marine single-celled microalgae has become a key link in meeting market demand.

[0003] Currently, existing marine single-celled microalgae propagation and cultivation devices have significant shortcomings in carbon dioxide supply. In most cultivation devices, carbon dioxide is directly transported into the device through pipes. This method of transportation results in a highly concentrated distribution of carbon dioxide in the culture medium, making it difficult to rapidly and evenly diffuse throughout the entire cultivation system. Due to the uneven distribution of carbon dioxide, the concentration of carbon source that microalgae in different areas of the culture medium comes into contact with varies, leading to uneven microalgae growth. Consequently, the harvested microalgae have inconsistent nutrient content, making it difficult to meet the stringent requirements of the food industry for raw material quality and stability.

[0004] Therefore, the present invention provides a marine single-celled microalgae propagation and cultivation device. Summary of the Invention

[0005] In order to overcome the shortcomings of the prior art, at least one technical problem raised in the background art is solved.

[0006] The technical solution adopted by the present invention to solve its technical problem is as follows: The marine single-celled microalgae propagation and cultivation device of the present invention includes a cultivation tank. An upper sealing plate is fixedly installed on the inner wall of the cultivation tank. A plurality of air guide pipes are uniformly fixedly installed on the inner wall of the upper sealing plate. One-way exhaust valves are fixedly installed at one end of each of the plurality of air guide pipes. The one-way exhaust valves are all located above the upper sealing plate. A carbon dioxide supply pipe is fixedly installed on the inner wall of the cultivation tank. The height of the carbon dioxide supply pipe is lower than the height of the upper sealing plate. A sealing component is provided below the upper sealing plate. An elastic component is provided between the sealing component and the cultivation tank.

[0007] The sealing assembly includes a lower sealing plate, which is located below the upper sealing plate. The height of the lower sealing plate is lower than the height of the carbon dioxide supply pipe. A sealing ring is fixedly installed on the outer wall of the lower sealing plate. The outer wall of the sealing ring is in contact with the inner wall of the culture tank. Several sealing covers are evenly fixedly installed on the top of the lower sealing plate. The several sealing covers are respectively fitted onto the end of the gas duct away from the one-way exhaust valve.

[0008] Preferably, the elastic component includes a telescopic rod, and several telescopic rods are uniformly and fixedly installed at the bottom of the lower sealing plate. The end of each telescopic rod away from the lower sealing plate is fixedly connected to the bottom of the inner wall of the culture tank. Several elastic elements are fixedly installed between the bottom of the lower sealing plate and the bottom of the inner wall of the culture tank, and several elastic elements are respectively sleeved on the outside of the telescopic rod.

[0009] Preferably, a plurality of sliding rods are fixedly installed on the top of the lower sealing plate, the outer walls of the sliding rods are slidably connected to the inner wall of the upper sealing plate, and a filter plate is fixedly installed between the plurality of sliding rods, the filter plate being located above the one-way exhaust valve.

[0010] Preferably, the inner wall of the culture tank is provided with a flow guiding component, and the outer side of the culture tank is provided with a flow diversion component. Under the action of the flow diversion component, the flow guiding component directly transports the culture medium from the upper part of the culture tank to the lower part.

[0011] Preferably, the flow guiding assembly includes drainage tubes, all of which are fixedly installed on the inner wall of the culture tank. The drainage tubes are arranged in a circular array with the center of the culture tank as the center. One-way liquid inlet valves are fixedly installed at one end of each drainage tube located inside the culture tank. The inner wall of each drainage tube is connected to a flow guiding tube. One end of each flow guiding tube extends to the inner side of the culture tank. One-way liquid drain valves are fixedly installed at the end of each flow guiding tube located inside the culture tank.

[0012] Preferably, the drainage assembly includes a sleeve, and several sleeves are provided and fixedly installed at the end of the drainage tube away from the one-way inlet valve. A piston is slidably installed on the inner wall of each of the sleeves. A transmission assembly is provided below the lower sealing plate. A filter ring is fixedly installed on the inner wall of the culture tank. The one-way inlet valve is located inside the filter ring.

[0013] Preferably, the transmission assembly includes a receiving rod, and several receiving rods are uniformly and fixedly installed at the bottom of the lower sealing plate. The outer walls of the receiving rods are slidably connected to the inner wall of the culture tank. A lifting plate is fixedly installed between the several receiving rods. Several connecting rods are fixedly installed on the top of the lifting plate. The ends of the several connecting rods away from the lifting plate are respectively fixedly connected to the inner wall of the piston.

[0014] Preferably, the inner walls of several sleeves are connected to drain pipes, the outer walls of the drain pipes are fixedly equipped with control valves, and the ends of the drain pipes away from the sleeves are fixedly equipped with nozzles.

[0015] Preferably, a mounting base is fixedly installed on the top of the culture tank, a top cover is attached to the top of the mounting base, a plurality of locking components are evenly fixedly installed on the outer wall of the mounting base, the top cover is fixedly connected to the mounting base through the locking components, and a plurality of support legs are fixedly installed on the bottom of the culture tank.

[0016] The beneficial effects of this invention are as follows:

[0017] 1. In the initial state, the sealing assembly seals the gas delivery pipe, and carbon dioxide first fills the space between the upper sealing plate and the sealing assembly. Once the space is full, as the gas supply continues, the pressure increases, squeezing the sealing assembly downwards and releasing the seal on the gas delivery pipe. Simultaneously, carbon dioxide is ejected from each one-way exhaust valve. This design avoids the concentrated release of carbon dioxide from one or a few fixed points, enabling carbon dioxide to be more evenly distributed to various areas of the incubator, effectively solving the problem of excessively high or low local carbon dioxide concentrations that may occur with traditional gas supply methods.

[0018] 2. The sealing sleeve covers the gas guide pipe. Carbon dioxide first accumulates in the space between the upper and lower sealing plates. As the carbon dioxide supply equipment continues to supply gas, the pressure in this space gradually increases. Only when the pressure reaches a level sufficient to overcome the resistance of the elastic component, causing the lower sealing plate to move down and the sealing sleeve covers to separate from the gas guide pipe, will the carbon dioxide be released. This design allows for flexible adjustment of the carbon dioxide release conditions according to actual needs, meeting the carbon dioxide concentration requirements of different stages of microalgae growth.

[0019] 3. When carbon dioxide is released, the filter plate moves up and down repeatedly under the action of the slide bar. Since the filter plate is located above the one-way exhaust valve, it cuts the carbon dioxide bubbles ejected from the one-way exhaust valve during its movement. This cutting action can break down larger carbon dioxide bubbles into smaller and denser bubbles. Smaller bubbles have a larger specific surface area, which means that the contact area between carbon dioxide and the culture medium is greatly increased, thereby improving the dissolution efficiency of carbon dioxide in the culture medium and enabling microalgae to make fuller use of carbon dioxide for photosynthesis. Attached Figure Description

[0020] The invention will now be further described with reference to the accompanying drawings.

[0021] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0022] Figure 2 This is a schematic diagram of the structure of the culture tank of the present invention;

[0023] Figure 3 This is a cross-sectional view of the culture tank structure of the present invention;

[0024] Figure 4 This is another structural cross-sectional view of the culture tank of the present invention;

[0025] Figure 5 This is a schematic diagram of the structure of the upper sealing plate of the present invention;

[0026] Figure 6 This is another structural schematic diagram of the upper sealing plate of the present invention;

[0027] Figure 7 This is a schematic diagram of the structure of the lower sealing plate of the present invention;

[0028] Figure 8 This is a schematic diagram of the air duct structure of the present invention;

[0029] Figure 9 This is a schematic diagram of the filter plate structure of the present invention;

[0030] Figure 10 This is a schematic diagram of the structure of the lifting plate of the present invention;

[0031] Figure 11 This is a schematic diagram of the drainage tube structure of the present invention;

[0032] Figure 12 This is a cross-sectional view of the sleeve structure of the present invention.

[0033] In the diagram: 1. Culture tank; 2. Upper sealing plate; 3. Gas guide pipe; 4. One-way exhaust valve; 5. Carbon dioxide supply pipe; 6. Lower sealing plate; 7. Sealing ring; 8. Sealing sleeve cover; 9. Telescopic rod; 10. Elastic element; 11. Sliding rod; 12. Filter plate; 13. Drainage pipe; 14. One-way liquid inlet valve; 15. Drainage pipe; 16. One-way liquid drain valve; 17. Sleeve; 18. Piston; 181. Filter ring; 19. Receiving rod; 20. Lifting plate; 21. Connecting rod; 22. Drainage pipe; 23. Control valve; 24. Nozzle; 25. Mounting base; 26. Top cover; 27. Locking element; 28. Support leg. Detailed Implementation

[0034] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0035] like Figures 1 to 9As shown in the embodiment of the present invention, a marine single-celled microalgae propagation and cultivation device includes a culture tank 1. An upper sealing plate 2 is fixedly installed on the inner wall of the culture tank 1. A plurality of air guide pipes 3 are uniformly fixedly installed on the inner wall of the upper sealing plate 2. One-way exhaust valves 4 are fixedly installed at one end of each air guide pipe 3, and the one-way exhaust valves 4 are all located above the upper sealing plate 2. A carbon dioxide supply pipe 5 is fixedly installed on the inner wall of the culture tank 1. The height of the carbon dioxide supply pipe 5 is lower than the height of the upper sealing plate 2. A sealing assembly is located below the upper sealing plate 2, and an elastic component is provided between the sealing assembly and the culture tank 1. The culture medium and other required components are placed in the culture tank 1 for marine single-celled microalgae propagation and cultivation. In the microalgae propagation and cultivation process, carbon dioxide supply pipe 5 is connected to a carbon dioxide supply device. When carbon dioxide gas needs to be injected into the cultivation tank 1 during the cultivation process, the carbon dioxide supply device injects carbon dioxide gas into the cultivation tank 1 through carbon dioxide supply pipe 5. The carbon dioxide gas first enters the space between the upper sealing plate 2 and the sealing assembly. Initially, all gas guide pipes 3 are sealed by the sealing assembly, so the carbon dioxide first fills the space between the upper sealing plate 2 and the sealing assembly. When the space between the upper sealing plate 2 and the sealing assembly is full of carbon dioxide, as the carbon dioxide supply device continues to supply gas, the space between the upper sealing plate 2 and the sealing assembly... As pressure increases, the elastic component's contractility allows the sealing component to move downwards. With increasing pressure, carbon dioxide compresses the sealing component, causing it to move downwards. During this downward movement, the sealing component releases the seal on each of the gas delivery pipes 3. At this time, carbon dioxide simultaneously passes through each gas delivery pipe 3 and is ejected through the one-way exhaust valves 4. In summary, initially, the sealing component seals the gas delivery pipes 3, and carbon dioxide first fills the space between the upper sealing plate 2 and the sealing component. Once the space is full, as gas supply continues, the pressure increases, compressing the sealing component to move downwards, releasing the seal on the gas delivery pipes 3, and carbon dioxide is simultaneously ejected from each one-way exhaust valve 4. This design avoids carbon dioxide... The concentrated release of carbon dioxide from one or a few fixed points allows for more uniform dispersion of carbon dioxide across various areas of the culture tank 1. This effectively solves the problem of excessively high or low local carbon dioxide concentrations that may occur with traditional gas supply methods. A uniform and sufficient supply of carbon dioxide provides the necessary conditions for microalgae to perform photosynthesis. Microalgae need to absorb carbon dioxide during photosynthesis. This invention ensures that microalgae in each area of ​​the culture tank 1 can obtain sufficient carbon dioxide, thereby improving photosynthetic efficiency, promoting the growth and reproduction of microalgae, and increasing the yield of microalgae. It should be noted that, in order to prevent liquid from entering below the upper sealing plate 2, the one-way exhaust valve 4 only allows gas to pass through.

[0036] like Figures 3 to 8As shown, the sealing assembly includes a lower sealing plate 6, which is positioned below the upper sealing plate 2. The height of the lower sealing plate 6 is lower than the height of the carbon dioxide supply pipe 5. A sealing ring 7 is fixedly installed on the outer wall of the lower sealing plate 6, and the outer wall of the sealing ring 7 is in contact with the inner wall of the culture tank 1. Several sealing covers 8 are evenly fixedly installed on the top of the lower sealing plate 6, and the sealing covers 8 are respectively fitted onto the end of the gas duct 3 away from the one-way exhaust valve 4. The sealing covers 8 are fixed above the lower sealing plate 6 to form a reliable sealing structure. In the initial state, each sealing cover 8 is fitted onto one end of each gas duct 3, thereby sealing each gas duct 3 and preventing carbon dioxide from passing through. When the pressure increases and the lower sealing plate 6 moves downward, the lower sealing plate 6 will move downward along with each sealing cover 8. When the sealing cap 8 moves down to separate from the gas delivery tube 3, the sealing cap 8 can no longer seal the gas delivery tube 3. Carbon dioxide will then pass through the gas delivery tube 3 and enter the culture medium through the one-way exhaust valve 4. Through the cooperation of the lower sealing plate 6 and the sealing cap 8, the timing of carbon dioxide release can be precisely controlled. Initially, the sealing cap 8 seals the gas delivery tube 3, and carbon dioxide first accumulates in the space between the upper sealing plate 2 and the lower sealing plate 6. As the carbon dioxide supply equipment continues to supply gas, the pressure in this space gradually increases. Only when the pressure reaches a level sufficient to overcome the resistance of the elastic component, causing the lower sealing plate 6 to move down and the sealing cap 8 to separate from the gas delivery tube 3, will the carbon dioxide be released. This design can flexibly adjust the carbon dioxide release conditions according to actual needs to meet the carbon dioxide concentration requirements of different stages of microalgae growth.

[0037] like Figures 4 to 5 and Figure 9 As shown, the elastic component includes a telescopic rod 9, several of which are uniformly and fixedly installed at the bottom of the lower sealing plate 6. The ends of the telescopic rods 9 away from the lower sealing plate 6 are fixedly connected to the bottom of the inner wall of the culture tank 1. Several elastic elements 10 are fixedly installed between the bottom of the lower sealing plate 6 and the bottom of the inner wall of the culture tank 1, and the elastic elements 10 are respectively sleeved on the outside of the telescopic rods 9. The telescopic rods 9 and elastic elements 10 are arranged between the lower sealing plate 6 and the culture tank 1. The contractility of the telescopic rods 9 and elastic elements 10 provides the conditions for the lower sealing plate 6 to move downward. The elastic elements 10 support the lower sealing plate 6. When the pressure between the upper sealing plate 2 and the lower sealing plate 6 can overcome the elasticity of the elastic elements 10, the lower sealing plate 6 will move downward. The telescopic rods 9 restrict the lower sealing plate 6 to move only in the vertical direction, avoiding its swaying in the horizontal direction, ensuring the smooth and accurate separation process of the sealing cover 8 and the gas guide tube 3, so that carbon dioxide can be released uniformly according to the design requirements.

[0038] like Figures 3 to 5 and Figure 9As shown, several sliding rods 11 are fixedly installed on the top of the lower sealing plate 6. The outer walls of the sliding rods 11 are slidably connected to the inner wall of the upper sealing plate 2. A filter plate 12 is fixedly installed between the sliding rods 11, and the filter plate 12 is located above the one-way exhaust valve 4. When releasing carbon dioxide, the carbon dioxide supply equipment first delivers carbon dioxide to the space between the upper sealing plate 2 and the lower sealing plate 6. As the pressure increases, the lower sealing plate 6 moves downward to release carbon dioxide. When the lower sealing plate 6 moves downward, it will drive the sliding rods 11 to move downward. When the sliding rods 11 move downward, they will drive the filter plate 12 downward. When the sealing sleeve cover 8 separates from the air guide pipe 3, the pressure between the upper sealing plate 2 and the lower sealing plate 6 decreases due to the release of carbon dioxide. The lower sealing plate 6 will move upwards, and simultaneously, the carbon dioxide supply device will draw in air, creating a negative pressure between the upper sealing plate 2 and the lower sealing plate 6. This negative pressure will cause the lower sealing plate 6 to move further upwards. As the lower sealing plate 6 moves upwards, it will drive the filter plate 12 upwards via the slide rod 11. This reciprocating motion allows the filter plate 12 to move up and down simultaneously while releasing carbon dioxide. Since the filter plate 12 is located above the one-way exhaust valve 4, it allows for better airflow. As the filter plate 12 reciprocates, it cuts the carbon dioxide bubbles. In summary, when carbon dioxide is released, the filter plate 12 moves up and down repeatedly under the action of the slide rod 11. Since the filter plate 12 is located above the one-way exhaust valve 4, it cuts the carbon dioxide bubbles ejected from the one-way exhaust valve 4 during its movement. This cutting action can break larger carbon dioxide bubbles into smaller and denser bubbles. Smaller bubbles have a larger specific surface area, which means that the contact area between carbon dioxide and the culture medium is greatly increased, thereby improving the dissolution efficiency of carbon dioxide in the culture medium and enabling microalgae to make fuller use of carbon dioxide for photosynthesis. The reciprocating motion of the filter plate 12 not only cuts the bubbles but also promotes the mixing of carbon dioxide gas in the culture medium. As the filter plate 12 moves up and down, it drives the flow of the surrounding culture medium, forming a local stirring effect. This stirring action helps to break the concentration gradient of carbon dioxide in the culture medium, so that carbon dioxide is more evenly distributed in all areas of the culture tank 1, avoiding the occurrence of local carbon dioxide concentrations that are too high or too low, and providing a more stable and uniform gas environment for microalgae.

[0039] like Figures 1 to 4 and Figures 10 to 12As shown, the inner wall of culture tank 1 is equipped with a flow guiding component, and the outer side of culture tank 1 is equipped with a flow drainage component. The flow guiding component, under the action of the flow drainage component, directly transports the culture medium from the upper part of culture tank 1 to the lower part. Gas supply and suction via the carbon dioxide supply component cause the lower sealing plate 6 to rise and fall. When the lower sealing plate 6 rises and falls, it, through the flow drainage component, causes the flow guiding component to directly transport the culture medium near the top of culture tank 1 to the bottom of culture tank 1, achieving circulation of the culture medium. During the cultivation process, microalgae continuously consume nutrients in the culture medium and produce metabolic products. If the culture medium does not circulate, nutrients in some areas of the culture medium will gradually decrease, metabolic products will accumulate, and there will also be... In some areas, the culture medium may have excessive nutrients or abnormal concentrations of metabolic products due to long-term underutilization. By using the flow guiding and drainage components to circulate the culture medium, it is possible to ensure that the culture medium is fully mixed in culture tank 1, providing a uniform nutrient environment for microalgae. This ensures that each microalgal cell receives sufficient nutrients, promoting its uniform growth. The growth of marine single-celled microalgae depends on light for photosynthesis. The circulating culture medium allows the microalgae to flow continuously in culture tank 1, preventing them from accumulating in one area for a long time and resulting in insufficient light utilization. When the culture medium circulates, microalgae in different locations have the opportunity to approach the light source and receive uniform light, improving photosynthetic efficiency and thus increasing the biomass accumulation of microalgae.

[0040] like Figures 1 to 4 and Figures 10 to 11As shown, the flow guiding assembly includes drainage tubes 13, all of which are fixedly installed on the inner wall of the culture tank 1. The drainage tubes 13 are arranged in a circular array with the center of the culture tank 1 as the center. One-way inlet valves 14 are fixedly installed at one end of each drainage tube 13 located inside the culture tank 1. The inner wall of each drainage tube 13 is connected to a guide tube 15, one end of which extends to the inner side of the culture tank 1. One-way drain valves 16 are fixedly installed at the inner end of each guide tube 15 located inside the culture tank 1. The one-way inlet valve 14 is located near the top of the culture medium, and the one-way drain valve 16 is located near the bottom of the culture medium. When the lower sealing plate 6 moves, it will drive the flow guiding assembly to move, and the flow guiding assembly will draw the culture medium above into the inner wall of the drainage tubes 13 through the one-way inlet valves 14. The culture medium is then drained through the one-way drain valve 16 using the guide tube 15. This process is repeated to directly transport the culture medium from the top of the culture tank 1 to the bottom, achieving vertical circulation of the culture medium. This allows nutrients such as nitrogen, phosphorus, and potassium in the culture medium to be evenly distributed within the culture tank 1. As microalgae continuously consume nutrients during cultivation, the circulating culture medium can promptly replenish the relatively nutrient-poor culture medium at the top to the bottom, while simultaneously bringing the nutrient-rich culture medium from the bottom to the top, providing a uniform nutrient environment for the microalgae. In addition, the uniformly circulating culture medium also allows the microalgae to move continuously within the culture tank 1, preventing them from accumulating in one area for a long time. This ensures that each microalgae cell receives uniform light, improving photosynthetic efficiency and promoting uniform growth of the microalgae.

[0041] like Figures 3 to 4 and Figures 10 to 12As shown, the drainage assembly includes several sleeves 17, each fixedly installed at the end of the drainage tube 13 away from the one-way inlet valve 14. Pistons 18 are slidably mounted on the inner walls of each sleeve 17. A transmission assembly is located below the lower sealing plate 6. Filter rings 181 are fixedly installed on the inner wall of the culture tank 1, and the one-way inlet valves 14 are all located inside the filter rings 181. When the lower sealing plate 6 is raised or lowered, it drives the pistons 18 to rise or fall via the transmission assembly. When the pistons 18 descend, a negative pressure is generated, drawing the culture medium into the drainage tube 13 through the one-way inlet valves 14. When the pistons 18 rise, they squeeze the culture medium in the drainage tube 13. After being squeezed, the culture medium is ejected through the guide tube 15 and the one-way drain valve 16, thus achieving vertical circulation of the culture medium. Furthermore, the ejection point of the culture medium is close to the location where carbon dioxide enters the culture medium. When the culture medium is at a certain pressure and... When ejected at high speed, it forms fine droplets and airflow, greatly increasing the contact area between carbon dioxide and the culture medium. This allows carbon dioxide to dissolve more quickly and fully in the culture medium, improving carbon dioxide utilization and providing a sufficient carbon source for microalgae photosynthesis. The ejected culture medium has a certain kinetic energy, which can create a good stirring effect at the bottom, further promoting the mixing and uniform distribution of the culture medium. Compared with natural flow or simple gravity drainage, squeeze drainage can more effectively break the stratification phenomenon in the culture medium and improve the efficiency of culture medium circulation. The filter ring 181 fixedly installed on the inner wall of the culture tank 1 can filter out larger particulate impurities, microalgae clumps, and some insoluble substances in the culture medium. The one-way inlet valve 14 is located inside the filter ring 181, so that the culture medium entering the drainage pipe 13 is filtered, reducing the risk of impurities clogging the drainage and guiding components and extending the service life of the equipment.

[0042] like Figures 2 to 4 and Figures 10 to 12As shown, the transmission assembly includes several support rods 19, which are evenly and fixedly installed at the bottom of the lower sealing plate 6. The outer walls of the support rods 19 are slidably connected to the inner wall of the culture tank 1. A lifting plate 20 is fixedly installed between the support rods 19. Several connecting rods 21 are fixedly installed on the top of the lifting plate 20. The ends of the connecting rods 21 away from the lifting plate 20 are respectively fixedly connected to the inner wall of the piston 18. When the lower sealing plate 6 rises and falls, it drives the support rods 19 to rise and fall. When the support rods 19 rise and fall, it drives the lifting plate 20 to rise and fall. During lifting and lowering, the piston 18 is driven to lift and lower via the connecting rod 21, providing power for the vertical circulation of the culture medium. It should be noted that the lifting and lowering of the lower sealing plate 6 is achieved by supplying and evacuating carbon dioxide using a carbon dioxide supply device. If carbon dioxide filling is not required, evacuation can be performed when the sealing cover 8 is not separated from the gas guide pipe 3. This allows for vertical circulation of the culture medium even without carbon dioxide filling. This feature makes the device more flexible and convenient to operate, allowing for the selection of whether to supply carbon dioxide based on actual conditions, while ensuring normal circulation of the culture medium.

[0043] like Figures 11 to 12 As shown, the inner walls of several sleeves 17 are all connected to drain pipes 22, and control valves 23 are fixedly installed on the outer walls of drain pipes 22. Spray nozzles 24 are fixedly installed at the end of drain pipes 22 away from sleeves 17. When the culture medium needs to be replaced, the culture medium inside the drainage pipe 13 also needs to be removed. At this time, the control valve 23 is opened to make the drain pipe 22 unobstructed. The culture medium in the drainage pipe 13 will be discharged through the spray nozzles 24 along the drain pipe 22, preventing the culture medium from remaining in the equipment, ensuring the thoroughness of the culture medium replacement, avoiding the potential risks caused by the mixing of new and old culture medium, and providing a purer and more suitable growth environment for microalgae.

[0044] like Figure 1 As shown, a mounting base 25 is fixedly installed on the top of the culture tank 1, and a top cover 26 overlaps the top of the mounting base 25. Several locking elements 27 are evenly fixedly installed on the outer wall of the mounting base 25. The top cover 26 is fixedly connected to the mounting base 25 through the locking elements 27. Several support legs 28 are fixedly installed on the bottom of the culture tank 1. The top cover 26 is fixed to the mounting base 25 through the locking elements 27. The mounting base 25 seals the culture tank 1 through the top cover 26, providing a relatively closed culture environment for the culture tank 1. When it is necessary to operate inside the culture tank 1, the locking elements 27 are released from the top cover 26, and the top cover 26 can be removed to operate inside the culture tank 1. The support legs 28 support the device, keeping the device in a suitable position while ensuring the overall stability of the device.

[0045] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A marine single-celled microalgae propagation and cultivation device, comprising a culture tank (1), characterized in that: The inner wall of the culture tank (1) is fixedly installed with an upper sealing plate (2). Several gas guide pipes (3) are evenly fixedly installed on the inner wall of the upper sealing plate (2). One-way exhaust valves (4) are fixedly installed at one end of each of the gas guide pipes (3). The one-way exhaust valves (4) are all located above the upper sealing plate (2). A carbon dioxide supply pipe (5) is fixedly installed on the inner wall of the culture tank (1). The height of the carbon dioxide supply pipe (5) is lower than the height of the upper sealing plate (2). A sealing component is provided below the upper sealing plate (2). An elastic component is provided between the sealing component and the culture tank (1). The sealing assembly includes a lower sealing plate (6), which is located below the upper sealing plate (2). The height of the lower sealing plate (6) is lower than the height of the carbon dioxide supply pipe (5). A sealing ring (7) is fixedly installed on the outer wall of the lower sealing plate (6). The outer wall of the sealing ring (7) is in contact with the inner wall of the culture tank (1). Several sealing covers (8) are evenly fixedly installed on the top of the lower sealing plate (6). Several sealing covers (8) are respectively fitted onto the end of the gas duct (3) away from the one-way exhaust valve (4). A number of slide rods (11) are fixedly installed on the top of the lower sealing plate (6). The outer walls of the slide rods (11) are slidably connected to the inner wall of the upper sealing plate (2). A filter plate (12) is fixedly installed between the slide rods (11). The filter plate (12) is located above the one-way exhaust valve (4).

2. The marine single-celled microalgae propagation and cultivation device according to claim 1, characterized in that: The elastic component includes a telescopic rod (9), which is provided in a plurality of uniformly fixed installations on the bottom of the lower sealing plate (6). The end of the telescopic rod (9) away from the lower sealing plate (6) is fixedly connected to the bottom of the inner wall of the culture tank (1). A plurality of elastic elements (10) are fixedly installed between the bottom of the lower sealing plate (6) and the bottom of the inner wall of the culture tank (1). The plurality of elastic elements (10) are respectively sleeved on the outside of the telescopic rod (9).

3. The marine single-celled microalgae propagation and cultivation device according to claim 1, characterized in that: The inner wall of the culture tank (1) is provided with a flow guiding component, and the outer side of the culture tank (1) is provided with a flow drainage component. Under the action of the flow drainage component, the flow guiding component directly transports the culture medium in the upper part of the culture tank (1) to the lower part.

4. The marine single-celled microalgae propagation and cultivation device according to claim 3, characterized in that: The flow guiding assembly includes a flow guiding tube (13), which is fixedly installed on the inner wall of the culture tank (1). The flow guiding tubes (13) are arranged in a circular array with the center of the culture tank (1) as the center. One-way liquid inlet valve (14) is fixedly installed at one end of each flow guiding tube (13) located inside the culture tank (1). The inner wall of each flow guiding tube (13) is connected to a flow guiding tube (15). One end of each flow guiding tube (15) extends to the inner side of the culture tank (1). One-way liquid drain valve (16) is fixedly installed at one end of each flow guiding tube (15) located inside the culture tank (1).

5. The marine single-celled microalgae propagation and cultivation device according to claim 4, characterized in that: The drainage assembly includes a sleeve (17), and several sleeves (17) are provided and fixedly installed at the end of the drainage tube (13) away from the one-way inlet valve (14). A piston (18) is slidably installed on the inner wall of each of the sleeves (17). A transmission assembly is provided below the lower sealing plate (6). A filter ring (181) is fixedly installed on the inner wall of the culture tank (1). The one-way inlet valve (14) is located inside the filter ring (181).

6. The marine single-celled microalgae propagation and cultivation device according to claim 5, characterized in that: The transmission assembly includes a receiving rod (19), and several receiving rods (19) are provided and uniformly fixedly installed at the bottom of the lower sealing plate (6). The outer wall of each receiving rod (19) is slidably connected to the inner wall of the culture tank (1). A lifting plate (20) is fixedly installed between several receiving rods (19). Several connecting rods (21) are fixedly installed on the top of the lifting plate (20). The ends of the several connecting rods (21) away from the lifting plate (20) are respectively fixedly connected to the inner wall of the piston (18).

7. The marine single-celled microalgae propagation and cultivation device according to claim 5, characterized in that: The inner walls of several sleeves (17) are connected to drain pipes (22), and control valves (23) are fixedly installed on the outer walls of the drain pipes (22). A nozzle (24) is fixedly installed at the end of the drain pipe (22) away from the sleeve (17).

8. The marine single-celled microalgae propagation and cultivation device according to claim 1, characterized in that: The top of the culture tank (1) is fixedly installed with a mounting base (25), and a top cover (26) overlaps the top of the mounting base (25). Several locking parts (27) are evenly fixedly installed on the outer wall of the mounting base (25). The top cover (26) is fixedly connected to the mounting base (25) through the locking parts (27). Several support legs (28) are fixedly installed at the bottom of the culture tank (1).

Images