Implant with honeycomb micro-nano topological structure on surface as well as preparation method and application of implant
By preparing a honeycomb-like micro/nano topological structure on the surface of a titanium-based implant and then subjecting it to hydrogenation treatment, the limitations of antibacterial coatings on the implant surface were overcome, achieving a non-invasive therapeutic effect that rapidly eliminates bacterial infection and promotes osseointegration.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- THE UNIVERSITY OF HONG KONG SHENZHEN HOSPITAL
- Filing Date
- 2024-10-31
- Publication Date
- 2026-05-01
AI Technical Summary
Existing antibacterial coatings on implant surfaces have problems such as limited capacity to carry antibacterial substances, difficulty in controlling antibacterial effects, and the risk of bacterial infection recurrence due to coating failure. Furthermore, traditional methods may have potential toxicity to normal cells.
A honeycomb-like micro/nano topological structure layer was prepared on the surface of a titanium-based implant using a template method, and then hydrogenated using a radio frequency plasma system to form a hydrogenated titanium dioxide surface with near-infrared light response. Near-infrared photocatalysis was used to generate reactive oxygen species to eliminate bacterial infection and regulate macrophage polarization.
It achieves rapid elimination of bacterial infection without secondary surgery, promotes bone integration, reduces inflammatory response, and provides a non-invasive and efficient implant surface modification strategy with the ability to eliminate bacterial infection and regulate bone integration through near-infrared light response.
Smart Images
Figure CN121944237A_ABST
Abstract
Description
An implant with a honeycomb-like micro / nano topological structure on its surface, its preparation method and application Technical Field
[0001] This invention belongs to the field of medical devices, specifically relating to an implant with a honeycomb-like micro-nano topological structure on its surface, its preparation method, and its application. Background Technology
[0002] Post-implantation, bacterial infections on the implant surface can form bacterial biofilms that are difficult to clear with antibiotics and the body's immune system. This can lead to revision surgeries, including debridement, prolonged antibiotic exposure, and implant removal, prolonging the treatment period and causing significant patient suffering. More seriously, the rapid evolution of drug-resistant bacteria further exacerbates this problem. To address this, one possible approach is to add an antimicrobial coating to the implant surface, loading it with antimicrobial substances such as antibiotics, metal ions, and antimicrobial peptides, which can inhibit bacterial adhesion or kill bacteria. Research on antimicrobial coatings has made some progress, for example, in the preparation of coatings with improved antimicrobial properties.
[0003] However, these existing coating surface modification methods still have certain limitations, including: 1) the total amount of antibacterial substances that the coating can carry is limited; 2) the antibacterial effect of the coating and its potential toxicity to normal cells depend on the release of the load, and achieving controlled on-demand release in the complex physiological environment in vivo remains challenging; 3) as the load is released or degraded, the coating will eventually fail, which may lead to recurrence of bacterial infection. Summary of the Invention
[0004] In view of this, the purpose of the present invention is to provide an implant with a honeycomb micro-nano topological structure on its surface, which solves the problem that patients currently need to undergo a second revision surgery.
[0005] Another objective of this invention is to provide a method for preparing an implant with a honeycomb-like micro / nano topological structure on its surface.
[0006] Another objective of this invention is to provide an application of the above-mentioned implant having a honeycomb micro / nano topological structure on its surface.
[0007] To achieve the above objectives, the first technical solution of the present invention is as follows: an implant with a honeycomb-like micro-nano topological structure on its surface is prepared on the surface of a titanium substrate by a template method to form a honeycomb-like micro-nano topological structure layer; the micro-nano topological structure layer has a photocatalytic effect on near-infrared light in the range of 805nm to 810nm.
[0008] Another technical solution of the present invention is achieved as follows: a method for preparing an implant with a honeycomb-like micro / nano topological structure on its surface, the preparation method specifically includes the following steps:
[0009] S1. Monodisperse polystyrene microspheres are used to form a monolayer polystyrene film on the surface of Ti(SO4)2 solution;
[0010] S2. The formed monolayer polystyrene film is heated at a temperature of 40℃~60℃ for 2.5h~3.5h to obtain a polystyrene microsphere film with titanium dioxide deposited at the bottom;
[0011] S3. The polystyrene microsphere film with titanium dioxide deposited at the bottom is transferred to a medical titanium substrate and calcined at high temperature for 1.5h to 2.5h, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0012] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0013] Preferably, in step S1, the size of the monodisperse polystyrene microspheres is 0.09 μm to 5 μm.
[0014] Preferably, in S1, the mass percentage of Ti(SO4)2 in the Ti(SO4)2 solution is 5.0%wt to 10.0%wt.
[0015] Preferably, in step S3, the high-temperature calcination temperature is 400℃~600℃.
[0016] Preferably, the heating rate during high-temperature calcination is 8–12 °C / min.
[0017] Preferably, in step S4, the conditions for hydrogenation using the radio frequency plasma system are: adjusting the hydrogen flow rate to 0 sccm to 20 sccm, the plasma power to 50 W to 100 W, the reaction temperature to 400 °C to 600 °C, and the reaction time to 1 h to 2 h.
[0018] The third technical solution of the present invention is implemented as follows: the application of an implant with a honeycomb micro-nano topological structure on the surface in the treatment of bone defect diseases.
[0019] Compared with existing technologies, this invention, through template method and plasma hydrogenation treatment, designs a unique hydrogenated titanium dioxide honeycomb surface micro-nano topological structure on the surface of titanium-based implants without introducing antibacterial coatings or complex chemical components. This special surface treatment not only possesses excellent 800-815nm near-infrared light-responsive photocatalytic performance, capable of generating a large amount of reactive oxygen species to rapidly eliminate bacterial infection, but its unique surface morphology can also regulate macrophage polarization, effectively inhibiting inflammatory responses and modulating the bone immune microenvironment to promote osteoogenesis. This effectively eliminates the need for secondary or multiple invasive surgeries, resulting in better postoperative recovery. Furthermore, this invention proposes for the first time a non-invasive and efficient surface modification strategy, enabling medical metal implants to possess the ability to eliminate bacterial infection and regulate bone integration through near-infrared light response. This novel method will provide a new treatment strategy for solving implant-related bacterial infections and insufficient bone integration, and is expected to bring significant clinical application value in the field of implants. Attached Figure Description
[0020] Figure 1 shows a microscope image of the titanium substrate with a honeycomb micro-nano topological structure on the surface in Example 1 of the present invention, and a microscope image of the implant with a honeycomb micro-nano topological structure on the surface after hydrogenation.
[0021] Figure 2 is a schematic diagram of the antibacterial efficiency of the implant with a honeycomb-like micro-nano topological structure on the surface obtained in Example 1 of the present invention under 808nm irradiation.
[0022] Figure 3 is a schematic diagram of the regulatory effect of the implant with a honeycomb-like micro-nano topological structure on macrophage gene expression obtained in Example 1 of the present invention. Detailed Implementation
[0023] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.
[0024] This invention provides an implant with a honeycomb-like micro / nano topological structure on its surface. A honeycomb-like micro / nano topological structure layer is prepared on the surface of a titanium substrate using a template method. The micro / nano topological structure layer exhibits photocatalytic activity in the near-infrared range of 800 nm to 815 nm.
[0025] This invention provides a method for preparing an implant with a honeycomb-like micro / nano topological structure on its surface. The method specifically includes the following steps:
[0026] S1. A monolayer polystyrene film is formed on the surface of a Ti(SO4)2 solution with a mass percentage of 5.0%wt to 10.0%wt by monodisperse polystyrene microspheres; the size of the monodisperse polystyrene microspheres is 0.09μm to 5μm; specifically 90nm, 100nm, 200nm, 500nm, 1μm or 5μm.
[0027] S2. The formed monolayer polystyrene film is heated at a temperature of 40℃~60℃ for 2.5h~3.5h to obtain a polystyrene microsphere film with titanium dioxide deposited at the bottom;
[0028] S3. The polystyrene microsphere film with titanium dioxide deposited on the bottom is transferred to a medical titanium substrate and calcined at a high temperature of 400℃~600℃ (heating rate of 8~12℃ / min) for 1.5h~3.05h, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0029] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system (PE-CVD) to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0030] The conditions for hydrogenation using the radio frequency plasma system are as follows: hydrogen flow rate is adjusted to 0 sccm to 20 sccm, plasma power is 50 W to 100 W, reaction temperature is 400 °C to 600 °C, and reaction time is 1 h to 2 h.
[0031] The present invention provides an application of an implant with a honeycomb-like micro / nano topological structure on its surface in the repair of bone defects.
[0032] The following are specific embodiments.
[0033] Example 1
[0034] An implant with a honeycomb-like micro / nano topological structure on its surface, as provided in Embodiment 1 of the present invention, is obtained through the following steps:
[0035] S1. A monolayer polystyrene film is formed on the surface of a Ti(SO4)2 solution with a mass percentage of 5.4% wt; the size of the monodisperse polystyrene microspheres is 90 nm.
[0036] S2. The formed monolayer polystyrene film is heated at 50°C for 3 hours to obtain a polystyrene microsphere film with titanium dioxide deposited on the bottom.
[0037] S3. The polystyrene microsphere film with titanium dioxide deposited at the bottom is transferred to a medical titanium substrate and calcined at a high temperature of 500°C (heating rate of 10°C / min) for 2 hours, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0038] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0039] The conditions for hydrogenation using the radio frequency plasma system are as follows: hydrogen flow rate is adjusted to 10 sccm, plasma power is 80 W, reaction temperature is 500 °C (adjusting the heating and cooling rates), and reaction time is 1.5 h.
[0040] Example 2
[0041] An implant with a honeycomb-like micro / nano topological structure on its surface, as provided in Embodiment 2 of the present invention, is obtained through the following steps:
[0042] S1. A monolayer polystyrene film is formed on the surface of a Ti(SO4)2 solution with a mass percentage of 5.4% wt; the size of the monodisperse polystyrene microspheres is 5 μm.
[0043] S2. The formed monolayer polystyrene film is heated at 50°C for 3 hours to obtain a polystyrene microsphere film with titanium dioxide deposited on the bottom.
[0044] S3. The polystyrene microsphere film with titanium dioxide deposited at the bottom is transferred to a medical titanium substrate and calcined at a high temperature of 500°C (heating rate of 10°C / min) for 2 hours, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0045] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0046] The conditions for hydrogenation using the radio frequency plasma system are as follows: hydrogen flow rate is adjusted to 10 sccm, plasma power is 80 W, reaction temperature is 500 °C (adjusting the heating and cooling rates), and reaction time is 1.5 h.
[0047] Example 3
[0048] An implant with a honeycomb-like micro / nano topological structure on its surface, as provided in Embodiment 3 of the present invention, is obtained through the following steps:
[0049] S1. A monolayer polystyrene film is formed on the surface of a Ti(SO4)2 solution with a mass percentage of 5.0% wt; the size of the monodisperse polystyrene microspheres is 200 nm.
[0050] S2. The formed monolayer polystyrene film is heated at 40°C for 3.5 hours to obtain a polystyrene microsphere film with titanium dioxide deposited on the bottom.
[0051] S3. The polystyrene microsphere film with titanium dioxide deposited on the bottom is transferred to a medical titanium substrate and calcined at a high temperature of 400℃ (8.0℃ / min) for 3.0h, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0052] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0053] The conditions for hydrogenation using the radio frequency plasma system are as follows: hydrogen flow rate is adjusted to 5 sccm, plasma power is 50 W, reaction temperature is 400 °C (adjusting the heating and cooling rates), and reaction time is 1 h.
[0054] Example 4
[0055] An implant with a honeycomb-like micro / nano topological structure on its surface, as provided in Embodiment 4 of the present invention, is obtained through the following steps:
[0056] S1. A monolayer polystyrene film is formed on the surface of a Ti(SO4)2 solution with a mass percentage of 10.0% wt; the size of the monodisperse polystyrene microspheres is 5 μm.
[0057] S2. The formed monolayer polystyrene film is heated at 60°C for 2.5 hours to obtain a polystyrene microsphere film with titanium dioxide deposited on the bottom.
[0058] S3. The polystyrene microsphere film with titanium dioxide deposited on the bottom is transferred to a medical titanium substrate and calcined at a high temperature of 600°C (12°C / min) for 1.5 hours, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0059] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0060] The conditions for hydrogenation using the radio frequency plasma system are as follows: hydrogen flow rate is adjusted to 20 sccm, plasma power is 100W, reaction temperature is 600℃ (adjusting the heating and cooling rates), and reaction time is 2h.
[0061] Example 5
[0062] An implant with a honeycomb-like micro / nano topological structure on its surface, as provided in Embodiment 5 of the present invention, is obtained through the following steps:
[0063] S1. A monolayer polystyrene film is formed on the surface of a Ti(SO4)2 solution with a mass percentage of 5.4% wt; the size of the monodisperse polystyrene microspheres is 90 nm.
[0064] S2. The formed monolayer polystyrene film is heated at 50°C for 3 hours to obtain a polystyrene microsphere film with titanium dioxide deposited on the bottom.
[0065] S3. The polystyrene microsphere film with titanium dioxide deposited on the bottom is transferred to a medical titanium substrate and calcined at a high temperature of 400℃ (heating rate of 8.0℃ / min) for 3.0h, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0066] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0067] The conditions for hydrogenation using the radio frequency plasma system are as follows: hydrogen flow rate is adjusted to 5 sccm, plasma power is 50 W, reaction temperature is 400 °C (adjusting the heating and cooling rates), and reaction time is 1 h.
[0068] Example 6
[0069] An implant with a honeycomb-like micro / nano topological structure on its surface, as provided in Embodiment 6 of the present invention, is obtained through the following steps:
[0070] S1. A monolayer polystyrene film is formed on the surface of a Ti(SO4)2 solution with a mass percentage of 5.4% wt; the size of the monodisperse polystyrene microspheres is 90 nm.
[0071] S2. The formed monolayer polystyrene film is heated at 50°C for 3 hours to obtain a polystyrene microsphere film with titanium dioxide deposited on the bottom.
[0072] S3. The polystyrene microsphere film with titanium dioxide deposited at the bottom is transferred to a medical titanium substrate and calcined at a high temperature of 600°C (heating rate of 12°C / min) for 1.5 hours, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0073] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0074] The conditions for hydrogenation using the radio frequency plasma system are as follows: hydrogen flow rate is adjusted to 20 sccm, plasma power is 100W, reaction temperature is 600℃ (adjusting the heating and cooling rates), and reaction time is 2h.
[0075] Example 7
[0076] An implant with a honeycomb-like micro / nano topological structure on its surface, as provided in Embodiment 7 of the present invention, is obtained through the following steps:
[0077] S1. A monolayer polystyrene film is formed on the surface of a Ti(SO4)2 solution with a mass percentage of 5.0% wt; the size of the monodisperse polystyrene microspheres is 200 nm.
[0078] S2. The formed monolayer polystyrene film is heated at 40°C for 3.5 hours to obtain a polystyrene microsphere film with titanium dioxide deposited on the bottom.
[0079] S3. The polystyrene microsphere film with titanium dioxide deposited at the bottom is transferred to a medical titanium substrate and calcined at a high temperature of 500°C (heating rate of 10°C / min) for 2 hours, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0080] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0081] The conditions for hydrogenation using the radio frequency plasma system are as follows: hydrogen flow rate is adjusted to 10 sccm, plasma power is 80 W, reaction temperature is 500 °C (adjusting the heating and cooling rates), and reaction time is 1.5 h.
[0082] Example 8
[0083] An implant with a honeycomb-like micro / nano topological structure on its surface, as provided in Embodiment 8 of the present invention, is obtained through the following steps:
[0084] S1. A monolayer polystyrene film is formed on the surface of a Ti(SO4)2 solution with a mass percentage of 10.0% wt; the size of the monodisperse polystyrene microspheres is 5 μm.
[0085] S2. The formed monolayer polystyrene film is heated at 60°C for 2.5 hours to obtain a polystyrene microsphere film with titanium dioxide deposited on the bottom.
[0086] S3. The polystyrene microsphere film with titanium dioxide deposited on the bottom is transferred to a medical titanium substrate and calcined at a high temperature of 400℃ (heating rate of 8.0℃ / min) for 3.0h, and then naturally cooled to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface.
[0087] S4. The titanium substrate with a honeycomb micro-nano topological structure on the surface is hydrogenated using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
[0088] The conditions for hydrogenation using the radio frequency plasma system are as follows: hydrogen flow rate is adjusted to 5 sccm, plasma power is 50 W, reaction temperature is 400 °C (adjusting the heating and cooling rates), and reaction time is 1 h.
[0089] Figure 1 shows a microscope image of the titanium substrate with a honeycomb micro-nano topological structure on the surface in Example 1, and a microscope image of the implant with a honeycomb micro-nano topological structure on the surface after hydrogenation. Analysis of Figure 1 shows that the surface morphology of the prepared titanium dioxide is a relatively uniformly distributed honeycomb pattern, and the average diameter of the honeycomb surface morphology is close to the size of the polystyrene template used in the preparation.
[0090] Figure 2 is a schematic diagram of the antibacterial efficiency of the implant with a honeycomb micro-nano topological structure obtained in Example 1 under 808nm irradiation. As can be seen from Figure 2, after only 15 minutes of light treatment, the implant with hydrogenated honeycomb micro-nano topological structure can quickly eliminate Staphylococcus aureus with an antibacterial rate of up to 99.93%, thus achieving rapid sterilization without introducing other antibacterial components.
[0091] Figure 3 is a schematic diagram of the regulatory effect of the implant with a honeycomb micro-nano topological structure on macrophage gene expression obtained in Example 1. Analysis of Figure 3 shows that these results indicate that the implant with a honeycomb micro-nano topological structure can induce M2 subtype macrophage polarization and inhibit M1 subtype macrophage polarization, which is beneficial to constructing an immune microenvironment that promotes bone integration.
[0092] Furthermore, current clinical antibacterial treatment primarily relies on antibiotics. Although antibiotics are effective in preventing and suppressing infection in the short term after surgery, recurrent infections after discontinuation of medication persist. Moreover, the rapid evolution of drug-resistant bacteria poses a significant challenge to traditional antibiotic therapy. Most antibacterial implants require the introduction of antibacterial agents or complex component coatings, but the instability and potential toxicity of these coatings adversely affect clinical application. This invention utilizes the inherent titanium dioxide component on the surface of titanium implants, eliminating the need for additional chemical components, resulting in implant surfaces with highly efficient near-infrared responsive antibacterial properties. This method not only rapidly eliminates surface bacteria under near-infrared photocatalysis for 15 minutes but also exhibits good stability and biocompatibility consistent with traditional titanium-based implants.
[0093] Furthermore, the preparation method of this invention creates a unique honeycomb-like nanostructure on the implant surface. This structure effectively modulates the immune response on the implant surface, thereby effectively inhibiting inflammation and promoting tissue regeneration. This unique nanostructure helps induce and regulate the immune response in vivo. This regulatory effect can effectively reduce the inflammatory response while promoting bone tissue regeneration and repair.
[0094] In summary, this invention, through template method and plasma hydrogenation treatment, designs a unique hydrogenated titanium dioxide honeycomb surface micro / nano topological structure on the surface of titanium-based implants without introducing antibacterial coatings or complex chemical components. This special surface treatment possesses excellent 808nm near-infrared photocatalytic performance, generating a large amount of reactive oxygen species to rapidly eliminate bacterial infection. Simultaneously, its unique surface morphology can regulate macrophage polarization, effectively inhibiting inflammatory responses and modulating the bone immune microenvironment to promote osteoogenesis. This effectively eliminates the need for secondary or multiple invasive surgeries, resulting in better postoperative recovery. Furthermore, this invention proposes for the first time a non-invasive and efficient surface modification strategy, enabling medical metal implants to possess near-infrared light-responsive capabilities to eliminate bacterial infection and immune-regulated bone integration. This novel method will provide a new treatment strategy for addressing implant-related bacterial infections and insufficient bone integration, and is expected to bring significant clinical application value in the field of implants.
[0095] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.
Claims
1. An implant with a honeycomb-like micro / nano topological structure on its surface, characterized in that, A honeycomb-like micro / nano topological structure layer was prepared on the surface of a titanium substrate using a template method; the micro / nano topological structure layer exhibits photocatalytic activity in the near-infrared range of 800 nm to 815 nm.
2. A method for preparing an implant with a honeycomb-like micro / nano topological structure on its surface as described in claim 1, characterized in that, The preparation method specifically includes the following steps: S1, forming a monolayer polystyrene film on the surface of a Ti(SO4)2 solution by placing monodisperse polystyrene microspheres; S2, heating the formed monolayer polystyrene film at a temperature of 40℃~60℃ for 2.5h~3.5h to obtain a polystyrene microsphere film with titanium dioxide deposited at the bottom; S3, transferring the polystyrene microsphere film with titanium dioxide deposited at the bottom to a medical titanium substrate, calcining it at high temperature for 1.5h~3.0h, and then naturally cooling it to room temperature to remove the polystyrene microsphere template, thereby obtaining a titanium substrate with a honeycomb micro-nano topological structure on the surface; S4, hydrogenating the titanium substrate with a honeycomb micro-nano topological structure on the surface using a radio frequency plasma system to obtain an implant with a honeycomb micro-nano topological structure on the surface.
3. The method for preparing an implant with a honeycomb-like micro / nano topological structure on its surface according to claim 2, characterized in that, In S1, the size of the monodisperse polystyrene microspheres is 0.09 μm to 5 μm.
4. The method for preparing an implant with a honeycomb-like micro / nano topological structure on its surface according to claim 3, characterized in that, In S1, the mass percentage of Ti(SO4)2 in the Ti(SO4)2 solution is 5.0%wt to 10.0%wt.
5. The method for preparing an implant with a honeycomb-like micro / nano topological structure on its surface according to claim 2, characterized in that, In step S3, the high-temperature calcination temperature is 400℃~600℃.
6. The method for preparing an implant with a honeycomb-like micro / nano topological structure on its surface according to claim 1 or 5, characterized in that, The heating rate during the high-temperature calcination is 8–12 °C / min.
7. The method for preparing an implant with a honeycomb-like micro / nano topological structure on its surface according to claim 2, characterized in that, In step S4, the conditions for hydrogenation using the radio frequency plasma system are as follows: the hydrogen flow rate is adjusted to 0 sccm to 20 sccm, the plasma power is 50 W to 100 W, the reaction temperature is 400 °C to 600 °C, and the reaction time is 1 h to 2 h.
8. The application of an implant with a honeycomb-like micro / nano topological structure on the surface as described in claim 1, or an implant with a honeycomb-like micro / nano topological structure on the surface obtained by any one of claims 2-7, in the treatment of bone defect diseases.