Organic fertilizer beneficial to seedling cultivation and preparation method

Abstract

The invention discloses an organic fertilizer beneficial to seedling cultivation, and belongs to the technical field of organic fertilizers, the organic fertilizer is a regular particle with a one-core four-layer structure, the one-core four-layer structure comprises a multifunctional composite core, the biological competition and activation layer, the moisture / stress sensing layer, the pH buffer release layer and the signal response layer sequentially wrap the multifunctional composite core from inside to outside; the multifunctional composite core comprises a humified organic carrier of an endophytic hypha network formed by pre-fermentation and colonization of trichoderma harzianum, and nutrient slow-release microcapsules dispersed in the humified organic carrier; the preparation method of the organic fertilizer beneficial to seedling cultivation comprises the steps of deep pretreatment of raw materials, construction of an endophytic hypha network core, preparation of a coating liquid, gradient coating, post-curing and finished product treatment. Through a one-core four-layer structure, five core functions of germination promotion and colonization, balanced fertilizer supply, root environment adjustment, stress protection and biological control required in a seedling growth period form a sensing-response-effect closed loop.

Description

Technical Field

[0001] This invention relates to the field of organic fertilizer technology, specifically to organic fertilizers that are beneficial for seedling cultivation and their preparation methods. Background Technology

[0002] Seedling cultivation is a crucial link in agricultural production, and strong seedlings are the foundation of a bountiful harvest. Currently, organic fertilizers used for seedling cultivation are mainly divided into several categories. The first category is commercial seedling substrates, which are stable and safe, but costly and have a fixed nutrient release pattern, making it difficult to meet the differentiated needs of different seedlings. The second category is traditional well-rotted farmyard manure (such as compost and oilseed cake), which, although low in cost and rich in nutrients, has inherent drawbacks such as difficulty in controlling the degree of decomposition, the potential to carry pathogens, excessively rapid or slow nutrient release, and uneven physicochemical properties. The third category is single-function additives (such as earthworm castings and microbial agents), which have significant effects but often have a single function and need to be compounded with other materials, increasing the complexity of their use.

[0003] Existing technologies mostly focus on static nutrient slow release or single-function addition, facing common bottlenecks such as rigid release mechanisms, inefficient functional synergy, one-sided substrate improvement, and extensive raw material processing. Specifically: 1. Rigid release mechanisms rely on water diffusion or simple time control, failing to respond to chemical signals secreted by roots (such as sugars and organic acids) and dynamic changes in rhizosphere pH and Eh (oxidation-reduction potential), leading to a disconnect between nutrient supply and physiological needs; 2. Inefficient functional synergy exists, with functional modules such as nutrient supply, microbial inoculation, disease inhibition, and stress mitigation often involving physical mixing, lacking spatiotemporal synergy based on mechanisms of action. The same design; for example, the release of growth-promoting bacteria may be earlier than their suitable rhizosphere colonization window, while the introduction of biocontrol bacteria may be delayed until the early stage of pathogen infection; 3. The substrate improvement is one-sided. Existing products are mostly used as additives, which are not capable of systematically constructing a three-dimensional healthy rhizosphere microenvironment of "physical looseness-chemical buffering-biological disease suppression", especially lacking proactive plans to cope with transplanting stress (drought, low temperature, salt damage); 4. The raw material processing is extensive. The modification of agricultural waste such as straw and seaweed residue is insufficient, and its structural and functional potential is not maximized; the composting process has a long cycle and poor stability, which affects product efficiency and consistency.

[0004] Therefore, developing a new type of organic fertilizer that integrates intelligent response release, multi-level structural support, deep modification of raw materials, and multi-functional spatiotemporal synergy, and can actively adapt to and optimize the microenvironment of the seedling rhizosphere throughout its entire growth cycle, has become a technical challenge that urgently needs to be overcome in this field. Summary of the Invention

[0005] The purpose of this invention is to provide an organic fertilizer and its preparation method that are beneficial to seedling cultivation, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] An organic fertilizer that is beneficial to seedling cultivation is a regular granule with a one-core and four-layer structure. The one-core and four-layer structure includes a multifunctional composite core and a fourth, third, second and first layer that wrap around the multifunctional composite core from the inside out.

[0008] The multifunctional composite core includes a humified organic carrier with an endogenous hyphal network formed by pre-fermentation and colonization of Trichoderma harzianum, and nutrient slow-release microcapsules dispersed therein; the humified organic carrier includes at least alkali-microwave synergistically modified straw fiber and ultrasonically pretreated seaweed residue.

[0009] The first layer is a signal response layer, which is composed of a polysaccharide-based gel material that is sensitive to plant root exudates, and contains growth-promoting microorganisms and seed germination stimulants.

[0010] The second layer is a pH buffer release layer, which is composed of pH-responsive polymer materials and loaded with humic acid-chelated trace elements and phosphorus- and potassium-solubilizing microbial agents.

[0011] The third layer is a moisture / stress sensing layer, which is composed of a smart gel material that is sensitive to changes in environmental moisture content or salt concentration, and is loaded with osmotic regulating substances and stress-resistant microbial agents.

[0012] The fourth layer is a biological competition and activation layer, which is composed of a gel material containing microbial quorum sensing inhibitors and specific beneficial microbial carbon sources.

[0013] As a further aspect of the present invention: the method for preparing the alkali-microwave synergistic modified straw fiber includes:

[0014] A. Soak the straw in a 2%-3% alkaline solution at 80-85℃ for 1.5-2.5 hours, then rinse until neutral;

[0015] B. Microwave treatment with a power of 300-500 W for 5-8 minutes;

[0016] C. Loading with humic acid and drying and pulverizing, the resulting modified straw fiber has a specific surface area ≥15 m². 2 / g.

[0017] As a further aspect of the present invention: the pretreatment parameters for the ultrasonically pretreated seaweed residue are as follows:

[0018] Frequency 25-40kHz;

[0019] Power 150-200W;

[0020] The processing time is 15-30 min, and the dissolution rate of seaweed polysaccharides after processing is not less than 60%.

[0021] As a further aspect of the present invention: the first layer is a signal response layer, the polysaccharide-based gel material is a complex of sodium alginate and pectin; the growth-promoting microorganism is a gelatinous Bacillus; and the germination stimulant is alginate oligosaccharide.

[0022] The second layer is a pH buffer release layer, and the pH-responsive polymer material is a complex of chitosan and humic acid; the trace elements include at least calcium, magnesium, iron, and zinc humic acid chelates; the phosphorus-solubilizing and potassium-solubilizing microbial agents include Pseudomonas fluorescens;

[0023] The third layer is a moisture / stress sensing layer. The smart gel material contains a thermosensitive polymer, poly(N-isopropylacrylamide), and / or a salt-sensitive polymer. The osmotic regulator includes proline or betaine. The stress-resistant microbial agent is a Bacillus subtilis strain with drought-resistant or salt-tolerant functions.

[0024] The fourth layer is a biological competition and activation layer, with furanone or its derivatives serving as the microbial quorum sensing inhibitor; and chitin oligosaccharides or chitosan oligosaccharides serving as the carbon source for specific beneficial microorganisms.

[0025] Another object of the present invention is to provide a method for preparing the above-mentioned organic fertilizer that is beneficial to seedling cultivation, comprising the following steps:

[0026] Step 1: Deep pretreatment of raw materials

[0027] Preparation of alkali-microwave synergistic modified straw fiber, ultrasonically pretreated seaweed residue and fully decomposed high-nitrogen organic material.

[0028] Step 2: Constructing the core of the endophytic hyphal network

[0029] The modified straw fiber, seaweed residue, well-rotted high-nitrogen organic material, mineral skeleton material, and humic acid obtained in step one are mixed and inoculated with Trichoderma harzianum for solid-state fermentation, allowing the mycelium to grow and colonize fully inside the carrier to form an endogenous network. After fermentation, the mixture is mixed with nutrient slow-release microcapsules and granulated to obtain multifunctional composite core particles. The solid-state fermentation conditions are a temperature of 25-30℃, a humidity of 55-65%, and a fermentation time of 5-7 days.

[0030] Step 3: Preparation of coating solution

[0031] Four functional coating solutions were prepared for forming the fourth, third, second, and first layers, respectively.

[0032] Step 4: Gradient Coating

[0033] In the fluidized bed coating equipment, the multifunctional composite core particles obtained in step two are sequentially coated with the fourth, third, second and first layers of spray coating, and each layer is dried and cured at a suitable temperature after coating.

[0034] Step 5: Post-ripening and Finished Product Processing

[0035] The coated granules are matured under controlled temperature and humidity conditions, then dried to the specified moisture content, and sieved to obtain the finished product.

[0036] Compared with the prior art, the beneficial effects of the present invention are:

[0037] 1. This invention is the first to integrate the response mechanisms of root exudates, pH, water / osmotic pressure and microbial community sensing to four key environmental factors (chemical, physical and biological) in a single fertilizer granule, achieving a revolutionary leap from passive slow release to multidimensional intelligent response. The release logic is highly consistent with the physiological state of seedlings and environmental stress.

[0038] This invention uses a four-layer structure to precisely sequence and coordinate the five core functions required in the seedling growth cycle—promoting germination and colonization, balancing fertilization, regulating root environment, protecting against stress, and biological control—forming a closed loop of perception-response-effectiveness and solving the problem of functional modules operating independently.

[0039] 2. The multifunctional composite core consists of a humified organic carrier and an endophytic mycelial network. It provides straw fiber with high specific surface area and water-holding porosity through alkali-microwave synergistic modification, and seaweed residue with ultrasonic crushing that provides natural biostimulants and colloids. The dual carriers and decomposed livestock and poultry manure are rapidly humified under the action of the composite microbial agent. During this process, Trichoderma harzianum is inoculated, and its mycelium pre-grows inside the granules to form an endophytic mycelial network. This achieves the provision of long-lasting basic fertility, excellent physical structure, and pre-established biocontrol advantages.

[0040] 3. The loaded plant signaling molecules will mimic the root environment and guide the Trichoderma harzianum hyphae to differentiate into rhizosphere-like behavior in the early stage of fermentation, inducing them to secrete large amounts of defensive enzymes such as chitinase and glucanase and secondary metabolites in advance; this makes the hyphal network in a pre-activated state of high disease resistance and growth promotion after colonization, rather than a dormant state.

[0041] The initial addition of fungal quorum sensing inhibitors will temporarily inhibit the excessive aggregation growth of hyphae, prompting hyphae to explore and colonize the carrier pores more evenly and deeply, thereby forming a three-dimensional network structure with better spatial distribution and stronger binding to the carrier, rather than surface hardening.

[0042] 4. Superparamagnetic iron oxide nanoparticles can be absorbed or attached to Trichoderma harzianum hyphae. Under the influence of a weak static magnetic field, the growth of hyphae is guided by magnetic field lines, thus growing more orderly and densely along the preset magnetic field direction, forming a magnetically responsive hyphal network with a specific orientation; this not only strengthens the network structure, but may also enhance the extension and exploration capabilities of the hyphae.

[0043] Magnetic nutrient slow-release microcapsules can be fixed or generate micro-movement in a magnetic field; in later applications, a remote, non-contact preliminary guidance of nutrient release in specific areas such as dense root zones can be achieved through an external controllable magnetic field; magnetic mycelial networks can serve as anchor points for the positioning and fixation of nutrient microcapsules in the soil.

[0044] 5. The g-C3N4 heterojunction can be activated under visible light. When fertilizer particles are exposed to light in the seedling greenhouse, the coating generates photogenerated electrons and holes, which can gently and continuously oxidize and decompose the complex organic matter on the surface and near the surface of the carrier, such as the recalcitrant humic acid macromolecules and lignin fragments, and convert them into small molecule organic acids and soluble nutrients, thus achieving on-demand activation and slow release driven by light energy.

[0045] The trace amounts of active oxygen (such as -OH) generated by photocatalysis can form a protective shell on the particle surface that is not conducive to the attachment of pathogenic microorganisms, reducing the risk of external pathogen infection; because the coating is extremely thin and its effect is limited to the surface layer, and the internal hyphal network is protected by the carrier, it will not harm the predetermined beneficial live bacteria such as Trichoderma harzianum inside.

[0046] The photocatalytic process can further decompose the residual seaweed macromolecules after ultrasonic pretreatment, releasing more active oligosaccharides and auxin-like substances, forming a synergistic stimulation effect with the small molecule nutrients produced by photolysis.

[0047] 6. The core technology adopts a multi-technology combination of physical, humic acid-loaded chemical, pre-fermentation colonization and biological deep modification process using alkali, microwave and ultrasound. This not only greatly enhances the added value of agricultural waste, but also creates a living functional carrier with a highly active endogenous mycelial network, making the biological function of fertilizer more durable and powerful.

[0048] By systematically integrating and optimizing refined raw material pretreatment, two-stage rapid humification, and gradient coating processes, an integrated preparation method capable of industrialization and stable production of complex structured particles has been formed.

[0049] 7. The oxygen produced by cyanobacteria can alleviate the hypoxia that may occur inside the core and promote the aerobic metabolism and activity of Trichoderma harzianum; the small molecule organic carbon secreted by it can directly provide high-quality carbon source for Trichoderma harzianum and outer functional microorganisms, forming endogenous nutrient supply.

[0050] Cyanobacteria photosynthesis alters the pH of the microenvironment, inducing the precipitation of minerals such as calcium carbonate. These minerals, deposited in the hyphal network and carrier pores, significantly enhance the mechanical strength of the core particles, preventing premature disintegration in the soil. This portion of carbon, sequestered in mineral form, is extremely stable, making each fertilizer granule a miniature carbon sink while fulfilling its function.

[0051] Integrating active photosynthetic carbon fixation and microbial-induced mineralization into fertilizer granules not only offsets part of the carbon footprint in production and transportation, but also may achieve net negative carbon emissions in the seedling stage, and form a virtuous cycle of energy and matter with the existing structure, which is a micro-practice of the concept of carbon-neutral agriculture.

[0052] 8. Trichoderma harzianum strains that successfully colonize and grow in a non-sterile, micro-competitive environment exhibit enhanced environmental adaptability, stress resistance, and resource competitiveness. The resulting core mycelial network is a superior microbial community honed through practical experience, demonstrating a greater colonization advantage in complex soil environments compared to strains cultured purely in the laboratory. Utilizing the amino acid mixture produced from the acidolysis of diseased or dead livestock and poultry to adjust the pH of the fermentation material eliminates the need for expensive high-temperature sterilization equipment and aseptic workshops, reducing the production cost of Trichoderma solid culture by an order of magnitude. This makes such a complex intelligent fertilizer economically feasible for large-scale industrialization and benefiting ordinary farmers. This shifts from pure culture that eliminates all competition to directional adaptive culture that "utilizes competition and eliminates the inferior," injecting stronger vitality into the multifunctional composite core. Detailed Implementation

[0053] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0054] Example 1

[0055] An organic fertilizer that is beneficial to seedling cultivation is a regular granule with a one-core and four-layer structure. The one-core and four-layer structure includes a multifunctional composite core and a fourth, third, second and first layer that wrap around the multifunctional composite core from the inside out.

[0056] The multifunctional composite core includes a humified organic carrier with an endogenous hyphal network formed by pre-fermentation and colonization of Trichoderma harzianum, and nutrient slow-release microcapsules dispersed therein; the humified organic carrier includes at least alkali-microwave synergistically modified straw fiber and ultrasonically pretreated seaweed residue.

[0057] The multifunctional composite core consists of a humified organic carrier and an endophytic mycelial network. It provides alkali-microwave synergistic modification of straw fiber with high specific surface area and water-holding porosity, and ultrasonically crushed seaweed residue that provides natural biostimulants and colloids. The dual carriers and decomposed livestock and poultry manure are rapidly humified under the action of the composite microbial agent. During this process, Trichoderma harzianum is inoculated, and its mycelium pre-grows inside the granules to form an endophytic mycelial network. This achieves the provision of long-lasting basic fertility, excellent physical structure, and pre-established biocontrol advantages.

[0058] The first layer is a signal response layer, which is composed of a polysaccharide-based gel material that is sensitive to plant root exudates, and contains growth-promoting microorganisms and seed germination stimulants.

[0059] The second layer is a pH buffer release layer, which is composed of pH-responsive polymer materials and loaded with humic acid-chelated trace elements and phosphorus- and potassium-solubilizing microbial agents.

[0060] The third layer is a moisture / stress sensing layer, which is composed of a smart gel material that is sensitive to changes in environmental moisture content or salt concentration, and is loaded with osmotic regulating substances and stress-resistant microbial agents.

[0061] The fourth layer is a biological competition and activation layer, which is composed of a gel material containing microbial quorum sensing inhibitors and specific beneficial microbial carbon sources.

[0062] This invention is the first to integrate the response mechanisms of root exudates, pH, water / osmotic pressure and microbial community sensing to four key environmental factors (chemical, physical and biological) in a single fertilizer granule, achieving a revolutionary leap from passive slow release to multidimensional intelligent response. The release logic is highly consistent with the physiological state of seedlings and environmental stress.

[0063] This invention uses a four-layer structure to precisely sequence and coordinate the five core functions required in the seedling growth cycle—promoting germination and colonization, balancing fertilization, regulating root environment, protecting against stress, and biological control—forming a closed loop of perception-response-effectiveness and solving the problem of functional modules operating independently.

[0064] Further:

[0065] The method for preparing the alkali-microwave synergistic modified straw fiber includes:

[0066] A. Soak the straw in a 2%-3% alkaline solution at 80-85℃ for 1.5-2.5 hours, then rinse until neutral;

[0067] B. Microwave treatment with a power of 300-500 W for 5-8 minutes;

[0068] C. Loading with humic acid and drying and pulverizing, the resulting modified straw fiber has a specific surface area ≥15 m². 2 / g.

[0069] Furthermore, in the step of loading humic acid in the preparation of alkali-microwave modified straw fiber, trace amounts of plant root-specific signaling molecules such as salicylic acid precursors, methyl jasmonate analogs, or chitin oligosaccharides are simultaneously loaded; and in the initial stage of Trichoderma harzianum pre-fermentation colonization, low concentrations of fungal quorum sensing inhibitors such as farnesol are added.

[0070] The loaded plant signaling molecules mimic the root environment, guiding the Trichoderma harzianum hyphae to differentiate into rhizosphere-like behaviors in the early stages of fermentation, inducing them to secrete large amounts of defensive enzymes such as chitinase and glucanase, as well as secondary metabolites in advance; this makes the hyphal network in a pre-activated state of high disease resistance and growth promotion after colonization, rather than a dormant state.

[0071] The initial addition of fungal quorum sensing inhibitors will temporarily inhibit the excessive aggregation growth of hyphae, prompting the hyphae to explore and colonize more evenly and deeply into the pores of the carrier, thereby forming a three-dimensional network structure with better spatial distribution and stronger binding to the carrier, rather than surface hardening.

[0072] Furthermore, the pretreatment parameters for the ultrasonically pretreated seaweed residue are as follows:

[0073] Frequency 25-40kHz;

[0074] Power 150-200W;

[0075] The processing time is 15-30 min, and the dissolution rate of seaweed polysaccharides after processing is not less than 60%.

[0076] Furthermore, the first layer is a signal response layer, the polysaccharide-based gel material is a complex of sodium alginate and pectin; the growth-promoting microorganism is a gelatinous Bacillus; and the germination stimulant is alginate oligosaccharide.

[0077] The second layer is a pH buffer release layer, and the pH-responsive polymer material is a complex of chitosan and humic acid; the trace elements include at least calcium, magnesium, iron, and zinc humic acid chelates; the phosphorus-solubilizing and potassium-solubilizing microbial agents include Pseudomonas fluorescens;

[0078] The third layer is a moisture / stress sensing layer. The smart gel material contains a thermosensitive polymer, poly(N-isopropylacrylamide), and / or a salt-sensitive polymer. The osmotic regulator includes proline or betaine. The stress-resistant microbial agent is a Bacillus subtilis strain with drought-resistant or salt-tolerant functions.

[0079] The fourth layer is a biological competition and activation layer, with furanone or its derivatives serving as the microbial quorum sensing inhibitor; and chitin oligosaccharides or chitosan oligosaccharides serving as the carbon source for specific beneficial microorganisms.

[0080] The first layer is the outermost layer, which is composed of a pectin-sodium alginate composite gel that is sensitive to specific root exudates (such as fructose and citric acid), and contains gel-like Bacillus spores and alginate oligosaccharides. After seed germination, the initial root exudates can trigger the rapid dissolution of this layer, releasing root-promoting bacteria and germination stimulants within 24-48 hours to seize the rhizosphere ecological niche.

[0081] The second layer is composed of a chitosan-humic acid composite membrane, which is loaded with humic acid-chelated micronutrients and phosphorus- and potassium-solubilizing functional bacteria. When the seedling root growth leads to acidification of the rhizosphere microzone (pH decrease), this layer dissolves more quickly and is precisely released on the 3rd to 15th day of growth to meet the needs of rapid growth and regulate the rhizosphere pH.

[0082] The third layer is composed of a composite gel of temperature-sensitive (such as poly(N-isopropylacrylamide) and salt-sensitive materials, and contains osmotic regulators such as proline and betaine, as well as drought-resistant / salt-resistant microorganisms (such as some Bacillus species). When the substrate moisture decreases or the salt concentration increases, the permeability of this layer changes or ruptures, releasing stress-resistant substances to help seedlings cope with environmental stress.

[0083] The fourth layer is the innermost layer, closely attached to the core, and is composed of a gel containing quorum sensing inhibitors and specific carbon sources. When the population of harmful rhizosphere bacteria reaches a certain density, the signaling molecules they release can accelerate the degradation of this layer, release substances that inhibit the sensing of harmful bacteria, and provide exclusive nutrition for beneficial bacteria such as Trichoderma harzianum in the core, thereby strengthening their biological control advantages.

[0084] Example 2

[0085] The only difference between this embodiment and Embodiment 1 is that:

[0086] During the preparation of the nutrient sustained-release microcapsules, superparamagnetic iron oxide nanoparticles are embedded in the wall material. During the pre-fermentation and colonization process of Trichoderma harzianum, a weak static magnetic field of 5-15 mT with a constant direction is applied to the reaction system.

[0087] Superparamagnetic iron oxide nanoparticles can be absorbed or attached to Trichoderma harzianum hyphae. Under the influence of a weak static magnetic field, the growth of hyphae is guided by magnetic field lines, thus growing more orderly and densely along the preset magnetic field direction, forming a magnetically responsive hyphal network with a specific orientation. This not only strengthens the network structure but may also enhance the extension and exploration capabilities of the hyphae.

[0088] Magnetic nutrient slow-release microcapsules can be fixed or generate micro-movement in a magnetic field; in later applications, a remote, non-contact preliminary guidance of nutrient release in specific areas such as densely rooted areas can be achieved through an external controllable magnetic field; magnetic hyphae networks can serve as anchor points for the positioning and fixation of nutrient microcapsules in the soil.

[0089] As a further aspect of the present invention: during the preparation of the humified organic carrier, an additional 0.5%-2% of a composite photosensitive material of graphitic carbon nitride and ferric humate is incorporated into the humified organic carrier; after the pre-fermentation of Trichoderma harzianum is completed and before the outer coating is applied, a photocatalytic interface construction step is added; the photocatalytic interface is constructed by vapor deposition or spraying onto the surface of the multifunctional composite core particles to form an ultrathin titanium dioxide or TiO2 / g-C3N4 heterojunction nanocoating.

[0090] The g-C3N4 heterojunction can be activated under visible light. When fertilizer particles are exposed to light in a seedling greenhouse, the coating generates photogenerated electrons and holes, which can gently and continuously oxidize and decompose complex organic matter such as recalcitrant humic acid macromolecules and lignin fragments on the surface and near the surface of the carrier, converting them into small molecule organic acids and soluble nutrients, thus achieving on-demand activation and slow release driven by light energy.

[0091] The trace amounts of active oxygen (such as -OH) generated by photocatalysis can form a protective shell on the particle surface that is not conducive to the attachment of pathogenic microorganisms, reducing the risk of external pathogen infection; because the coating is extremely thin and its effect is limited to the surface layer, and the internal hyphal network is protected by the carrier, it will not harm the predetermined beneficial live bacteria such as Trichoderma harzianum inside.

[0092] The photocatalytic process can further decompose the residual seaweed macromolecules after ultrasonic pretreatment, releasing more active oligosaccharides and auxin-like substances, forming a synergistic stimulation effect with the small molecule nutrients produced by photolysis.

[0093] Example 3

[0094] The only difference between this embodiment and Embodiment 1 is that:

[0095] In the preparation of the humified organic carrier, photosynthetic cyanobacteria such as Synechococcus are co-inoculated with Trichoderma harzianum, and a special hydrogel such as a printable gel is selected as the common substrate for the hyphae and cyanobacteria. The cyanobacteria use light, water and CO2 in the air to perform photosynthesis, producing oxygen and organic carbon.

[0096] The oxygen produced by cyanobacteria can alleviate the hypoxia that may occur inside the core and promote the aerobic metabolism and activity of Trichoderma harzianum; the small molecule organic carbon secreted by it can directly provide high-quality carbon source for Trichoderma harzianum and outer functional microorganisms, forming endogenous nutrient supply.

[0097] Cyanobacteria photosynthesis alters the pH of the microenvironment, inducing the precipitation of minerals such as calcium carbonate. These minerals, deposited in the hyphal network and carrier pores, significantly enhance the mechanical strength of the core particles, preventing premature disintegration in the soil. This portion of carbon, sequestered in mineral form, is extremely stable, making each fertilizer granule a miniature carbon sink while fulfilling its function.

[0098] Integrating active photosynthetic carbon fixation and microbial-induced mineralization into fertilizer granules not only offsets part of the carbon footprint in production and transportation, but also may achieve net negative carbon emissions during the seedling stage, and form a virtuous cycle of energy and matter with the existing structure, which is a micro-practice of the concept of carbon-neutral agriculture.

[0099] Example 4

[0100] The only difference between this embodiment and Embodiment 1 is that:

[0101] In the aforementioned Trichoderma harzianum pre-fermentation colonization, the mixture of amino acids produced by the acid hydrolysis of diseased and dead livestock and poultry is used to adjust the pH value of the fermentation material, creating a selective fermentation environment that is only beneficial to Trichoderma harzianum and inhibits most other bacteria.

[0102] Trichoderma harzianum strains that successfully colonize and grow in a non-sterile, micro-competitive environment exhibit enhanced environmental adaptability, stress resistance, and resource competitiveness. The resulting core mycelial network, a superior microbial community honed through practical experience, demonstrates greater colonization advantages in complex soil environments compared to strains cultured purely in the laboratory. Utilizing the amino acid mixture produced from the acidolysis of diseased or dead livestock to adjust the pH of the fermentation material eliminates the need for expensive high-temperature sterilization equipment and sterile workshops, reducing the production cost of solid Trichoderma harzianum strains by an order of magnitude. This makes such a complex intelligent fertilizer economically feasible for large-scale industrialization and benefit for ordinary farmers. This shifts from pure cultivation that eliminates all competition to directional adaptive cultivation that "utilizes competition and eliminates the inferior," injecting stronger vitality into the multifunctional composite core.

[0103] Example 5

[0104] This embodiment provides a method for preparing organic fertilizer that is beneficial to seedling cultivation, including the following steps:

[0105] Step 1: Deep pretreatment of raw materials

[0106] Preparation of alkali-microwave synergistic modified straw fiber, ultrasonically pretreated seaweed residue and fully decomposed high-nitrogen organic material.

[0107] Step 2: Constructing the core of the endophytic hyphal network

[0108] The modified straw fiber, seaweed residue, well-rotted high-nitrogen organic material, mineral skeleton material, and humic acid obtained in step one are mixed and inoculated with Trichoderma harzianum for solid-state fermentation, allowing the mycelium to grow and colonize fully inside the carrier to form an endogenous network. After fermentation, the mixture is mixed with nutrient slow-release microcapsules and granulated to obtain multifunctional composite core particles. The solid-state fermentation conditions are a temperature of 25-30℃, a humidity of 55-65%, and a fermentation time of 5-7 days.

[0109] Step 3: Preparation of coating solution

[0110] Four functional coating solutions were prepared for forming the fourth, third, second, and first layers, respectively.

[0111] Step 4: Gradient Coating

[0112] In the fluidized bed coating equipment, the multifunctional composite core particles obtained in step two are sequentially coated with the fourth, third, second and first layers of spray coating, and each layer is dried and cured at a suitable temperature after coating.

[0113] Step 5: Post-ripening and Finished Product Processing

[0114] The coated granules are matured under controlled temperature and humidity conditions, then dried to the specified moisture content, and sieved to obtain the finished product.

[0115] Example 6

[0116] The only difference between this embodiment and Embodiment 1 is that:

[0117] A method for preparing an organic fertilizer that is beneficial for seedling cultivation includes the following steps:

[0118] Step 1: Deep pretreatment of raw materials

[0119] Preparation of modified straw fiber: Corn straw was crushed to 2-3 cm, soaked in 2.5% NaOH solution at 85 ℃ for 2 h, and rinsed with water until neutral; after draining, it was placed in a microwave device and treated at 450 W power for 6 min to quickly dry and form a porous structure; then 5% humic acid solution was sprayed in and stirred for 2 h for adsorption, dried, crushed and passed through a 60 mesh sieve, and the specific surface area was measured to be ≥18 m². 2 / g;

[0120] Preparation of activated seaweed residue: The dried seaweed residue was crushed, mixed with water at a solid-liquid ratio of 1:10, and placed in an ultrasonic processor. It was treated for 25 min at a frequency of 35 kHz and a power of 180 W. After filtration, the solid part was dried, pulverized, and passed through a 100-mesh sieve. The seaweed polysaccharide dissolution rate was found to be 65%.

[0121] Preparation of decomposed high-nitrogen organic materials: Mix soybean meal and sheep manure at a ratio of 3:7, inoculate with compound microbial agents (cellulase, protease), and ferment aerobically at 55-60 ℃ for 7 days until fully decomposed, with a carbon-nitrogen ratio of approximately 12:1.

[0122] Step 2: Constructing the core of the endophytic hyphal network

[0123] Take 30 parts by weight of the modified straw fiber, 10 parts of activated seaweed residue, 25 parts of decomposed high-nitrogen organic material, 12 parts of mineral skeleton material (calcined oyster shell powder: diatomaceous earth = 1:1), and 2 parts of humic acid, and mix them evenly; inoculate with Trichoderma harzianum T-10 inoculant (1×108 1.2 parts of CFU / g were added, and the moisture content was adjusted to 58%. The mixture was then fermented in a solid state at 28℃ in the dark for 6 days. During the fermentation process, Trichoderma harzianum mycelium penetrated deep into the pores of the straw fibers and the entire material, forming a dense endogenous network. After fermentation, the material was mixed with 5 parts of nutrient slow-release microcapsules (chitosan-sodium alginate wall material, containing humic acid chelated NPK) and granulated into wet particles with a diameter of 3.5 mm. The particles were then dried at 50℃ to a moisture content of 30% to obtain multifunctional composite core particles.

[0124] Step 3: Preparation of coating solution

[0125] Signal response layer solution: Prepare a mixed solution of 3% sodium alginate and 2% citrus pectin, add 1.5% alginate oligosaccharide and 0.8% lyophilized Bacillus lentinans powder (2×10¹). 0 (CFU / g), mix well;

[0126] pH buffer release layer: Mix 2% chitosan (dissolved in 1% acetic acid) and 3% potassium humate solution at a volume ratio of 1:1, add humic acid-chelated trace elements Ca, Mg, Fe, and Zn (total concentration 2%) and 1% lyophilized phosphate-solubilizing bacteria powder, and mix well.

[0127] Moisture / stress-sensing layer: Prepare a composite solution containing thermosensitive materials poly(N-isopropylacrylamide) (PNIPAM, 1.5%) and carboxymethyl cellulose (CMC, 2%), and add 1% proline and 0.5% lyophilized powder of Bacillus subtilis specific stress-resistant strains.

[0128] Biological competition and activation layer solution: Prepare a 2% sodium alginate solution, add 0.1% furanone (a quorum sensing inhibitor analog) and 2% chitin oligosaccharide (a carbon source preferred by Trichoderma harzianum), and mix well.

[0129] Step 4: Gradient Coating

[0130] A fluidized bed coating machine with a precision temperature control and atomization system is used to preheat the core particles obtained in step two to 35°C before feeding them in.

[0131] First layer: Spraying signal response liquid, with an inlet air temperature of 40 ℃, to rapidly gel the surface layer, resulting in a dry film weight gain of approximately 8%;

[0132] Second layer: After the first layer has cured, spray the pH buffer release layer liquid, with an inlet air temperature of 42 ℃, and the dry film weight gain is about 15%;

[0133] Third layer: Spray moisture / stress-sensing liquid, inlet air temperature 38℃ (below the minimum critical dissolution temperature of PNIPAM), dry film weight gain approximately 10%;

[0134] Fourth layer: Spraying biological competition and activation layer liquid, inlet air temperature 40 ℃, dry film weight gain approximately 7%;

[0135] After each layer of spraying, allow the particles to flow and dry for 5-10 minutes until the surface is completely cured.

[0136] Step 5: Post-ripening and Finished Product

[0137] After coating, the particles are placed in a curing room at 32 ℃ and 75% relative humidity for 36 h to stabilize the structure of each membrane layer and ensure tight bonding with the core. Finally, they are dried in a ventilated environment at 40 ℃ until the moisture content is ≤12%, and the finished particles with a particle size of 4.0±0.5 mm are obtained by sieving and sealed packaging.

[0138] In summary, the core technology of deep modification of raw materials, which combines physical, humic acid-loaded chemical, pre-fermentation and colonization biological processes using alkali, microwave and ultrasound, not only greatly enhances the added value of agricultural waste, but also creates a living functional carrier with a highly active endogenous mycelial network, making the biological function of fertilizer more durable and powerful.

[0139] By systematically integrating and optimizing refined raw material pretreatment, two-stage rapid humification, and gradient coating processes, an integrated preparation method capable of industrialization and stable production of complex structured particles has been formed.

[0140] By adjusting the specific proportions of functional substances in each layer (such as the combination of trace elements and the types of stress-resistant agents), a series of specialized products suitable for different seedling types such as vegetables, flowers, and trees can be developed under the same technical platform, achieving a combination of standardized production and personalized customization.

[0141] To systematically and quantitatively verify the creative breakthrough of the present invention (Examples 1-4) over the existing technical bottlenecks, this experimental design follows the principles of the National Standard for Biological Organic Fertilizer (GB / T 20287-2006) and the Technical Regulations for Field Trials for Fertilizer Effect Identification (NY / T 497-2002), and constructs a rigorous control test scheme from three levels: product performance, mechanism of action, and application effect.

[0142] I. Overall Experimental Design

[0143] 1. Test materials:

[0144] 1.1. Experimental Group

[0145] Experimental group 1 is the "one core, four layers" organic fertilizer prepared in Example 1.

[0146] Experimental group 2 is the organic fertilizer prepared in Example 2.

[0147] Experimental group 3 is the organic fertilizer prepared in Example 3.

[0148] Experimental group 4 is the organic fertilizer prepared in Example 4.

[0149] 1.2. Control Group

[0150] Control group 1: Commercially available ordinary decomposed sheep manure organic fertilizer (representing conventional products with "static nutrient release and extensive raw material processing"); according to GB / T 19524.1-2004, its fecal coliform count did not meet the standard.

[0151] Control group 2: Commercially available general-purpose bio-organic fertilizer (containing only Bacillus subtilis, representing a product with "single-function addition and low efficiency of synergistic function"); its microbial function is singular.

[0152] Control group 3: Blank seedling substrate without any added organic fertilizer (peat:vermiculite = 7:3, volume ratio).

[0153] Test crop: Tomato, which is sensitive to the rhizosphere environment and has a suitable growth cycle, was selected as the model crop.

[0154] 2. Seedling raising and treatment setup

[0155] 72-cell trays were used for seedling cultivation. Each organic fertilizer was mixed evenly with the blank substrate (CK3) at a volume ratio of 10%. Each treatment was replicated 3 times, with 24 seedlings per replicate.

[0156] All treatments were conducted in the same intelligent greenhouse, uniformly irrigated with clean water, and no other fertilizers or pesticides were applied, in order to purely examine the function of each organic fertilizer itself.

[0157] 3. Core Observations and Stress-Induced Time Points

[0158] Routine observations: Day 3 after sowing (germination period), Day 15 (rapid seedling growth period), and Day 30 (mature seedling period).

[0159] Stress-induced observation: On day 30, half of the seedlings were subjected to drought stress (stop watering for 7 days) and low temperature stress (5℃ treatment for 3 days) to simulate transplanting stress.

[0160] II. Experimental Data

[0161] Table 1

[0162] experiment Test methods and standards Data Results and Analysis Chemical signal responsiveness On the third day after sowing, artificial root exudates (a mixture of 0.1% citric acid and 0.05% glucose) were used for irrigation. Two hours later, the number of viable Bacillus jelly-like bacteria in the seedling substrate surged. The selective culture medium plate counting method was used (refer to GB / T20287-2006). <![CDATA[The viable count of the experimental group 1 increased sharply from 10 5 CFU / g to over 10 7 CFU / g, while there were no significant changes in control group 1 and control group 2. This directly proves that the signal response layer of experimental group 1 can be rapidly activated by root chemical signals, enabling the "demand-driven release" of growth-promoting bacteria, and overcoming the rigid mechanism of existing fertilizers that rely on water diffusion for release.]]> pH response and nutrient release in sync Fifteen days after sowing, the pH of the rhizosphere soil was measured, and the concentrations of water-soluble calcium and magnesium ions in the substrate extract were simultaneously detected. The rhizosphere acidification process was simulated by adding dilute acid, and the dynamics of ion release were continuously monitored. pH was measured using the microelectrode method; ion concentration was measured using the ICP-OES method (refer to NY / T 1121.13). When the rhizosphere pH naturally decreased from 7.0 to 6.5, the release rate of calcium and magnesium ions in experimental group 1 increased by 180-220%, and the release curve closely matched the pH decrease curve. Control groups 1 and 2 showed slow release and no response peak. This result confirms that the pH buffer release layer can sense rhizosphere physiological changes, achieving dynamic synchronization between nutrient supply and plant demand. Drought Stress Response and Material Release After 30 days of drought stress treatment, the proline content in the leaves was measured, and the structural integrity of the third layer (water / stress-sensing layer) of the fertilizer granules was observed by dissection. Proline was determined by the sulfosalicylic acid method; particle profiles were observed by scanning electron microscopy. Under drought stress, the proline content in the leaves of experimental group 1 was 70-90% higher than that in control group 2. Electron microscopy showed that the third layer of the particles had a large number of directional cracks, while the control group had an intact structure. This indicates that the third layer can sense water stress and actively break down to release stress-resistant substances, demonstrating an intelligent response to environmental physical signals.

[0163] Table 2

[0164] experiment Test methods and standards Data Results and Analysis Sequential colonization patterns of functional microorganisms Samples were taken on days 3, 15, and 30, and high-throughput sequencing was used to analyze the rhizosphere microbial community structure and track the dynamic changes of key functional bacteria such as Bacillus jellyoidus, Pseudomonas fluorescens, and Trichoderma harzianum. 16S rRNA / ITS gene sequencing analysis. The rhizosphere microbiota in experimental group 1 exhibited a clear temporal succession: on day 3, it was dominated by root-promoting, gelatinous Bacillus; on day 15, the abundance of phosphorus- and potassium-solubilizing Pseudomonas fluorescens reached its peak; and on day 30, the natural defense agent Trichoderma harzianum became dominant. In contrast, the microbiota in control group 2 was chaotic and lacked a temporal pattern. This perfectly verifies that the "one-core, four-layer" structure enables a "relay race" synergy of functional bacteria, solving the problem of mismatch between the release of microbial agents and the demand window in existing technologies. Synergy and Foresight of Biological Control Functions On day 25 (before seedling establishment), the soil-borne pathogen Rhizoctonia solani was actively inoculated. On day 30, the disease incidence was investigated, and the content of pathogen quorum sensing signal molecules in the rhizosphere soil was measured. The disease index was calculated; LC-MS / MS was used to detect AHL-type quorum sensing signal molecules. The disease index in experimental group 1 was reduced by 65-75% compared to control group 2, and the concentration of quorum sensing signal molecules of rhizosphere harmful bacteria was suppressed by more than 55%. This indicates that the fourth layer (biological competition and activation layer) can not only release Trichoderma harzianum, but also proactively intervene in disease occurrence by inhibiting the "communication" of pathogens, achieving a multi-level synergistic defense of antibacterial and pathogenic interference.

[0165] Table 3

[0166] experiment Test methods and standards Data Results and Analysis Improvement of the root physical structure system After the seedling stage (day 30), the total porosity, aeration porosity, and water-holding porosity of the substrate were measured. The root morphology of the seedlings was also compared. Soil physical properties were determined by the ring cutter method (refer to NY / T1121.19); root scanner analysis was performed. The aeration porosity of the substrate in experimental group 1 was 30-40% higher than that in control group 1, and the water-holding porosity was 20-30% higher, resulting in a more balanced water-air ratio. The total root length and root surface area of ​​the seedlings in experimental group 1 increased by more than 50% compared to control group 2. This demonstrates that the dual-carrier core (modified straw fiber + seaweed residue) not only provides nutrients but also fundamentally and systematically constructs an ideal rhizosphere physical environment that is "loose, breathable, water-retentive, and fertile." Chemical environment buffering and stable fertilizer supply Throughout the seedling stage, the fluctuations in substrate pH and conductivity were monitored regularly, and the contents of available nitrogen, phosphorus, and potassium were measured on days 7, 15, and 30 to plot nutrient release kinetic curves. pH and EC were measured using potentiometry; readily available nutrients were measured using a continuous flow analyzer. In experimental group 1, the substrate pH remained consistently within the suitable range of 6.2-6.8, with no drastic fluctuations in EC values. Nutrient release exhibited a stable linear slow-release pattern, without early salt damage peaks or late nutrient deficiency troughs. In contrast, control group 1 experienced excessively high levels of readily available nutrients in the early stages, easily leading to seedling burn. This demonstrates the synergy between the core slow-release mechanism and the pH buffer layer, achieving a stable chemical environment. Overall effect of stress resistance and seedling strengthening Drought / low temperature stress was applied on day 30, and the survival rate, recovery growth rate and final seedling strength index of each treatment were measured 7 days later. Strong seedling index = (stem diameter / plant height + underground dry weight / above-ground dry weight) × total plant dry weight (refer to GB / T23416.2). After stress, the survival rate of seedlings in experimental group 1 was 40% higher than that in control group 2, and the recovery growth rate was 50% faster. The final seedling vigor index in experimental group 1 reached over 0.045, significantly higher than 0.028 in control group 2 and 0.019 in control group 1. This indicates that the present invention, through the synergistic effect of multiple physical, chemical, and biological improvements, endows seedlings with inherent health qualities and strong stress resistance, which cannot be achieved by any single-function additive.

[0167] Table 4

[0168] experiment Test methods and standards Data Results and Analysis Functional enhancement of carrier materials The specific surface area, water holding capacity, and adsorption properties of the modified straw fiber prepared according to this invention were directly measured and compared with those of straw that was only mechanically crushed. The content of seaweed polysaccharides and endogenous auxins in ultrasonically treated seaweed residue was also determined. Specific surface area was determined by BET nitrogen adsorption method; water holding capacity and adsorption amount were determined by physicochemical methods; and auxin was determined by high performance liquid chromatography. This invention achieves a specific surface area of ​​18-22 m² / g and a water holding capacity exceeding 400% in straw modified by alkali-microwave synergistic treatment, which are 9 times and 3 times that of traditionally crushed straw, respectively. Ultrasonic treatment of seaweed residue resulted in a polysaccharide dissolution rate of 65% and an auxin content increase of 8 times. This demonstrates that the pretreatment process of this invention represents a qualitative and profound modification, greatly stimulating the structural and biostimulatory potential of the raw materials, rather than simple crushing. Fermentation process efficiency and product stability Record the time required for Example 1 and conventional compost to reach complete decomposition (odorless, dark brown, C / N < 20). Also, test the safety indicators of the finished product. Observation records; fecal coliform count and ascarid egg mortality were measured according to GB / T 19524.1-2004. The rapid humification process used in Example 1 has a composting cycle of 15-18 days, while traditional composting requires more than 60 days. All experimental groups produced fecal coliform counts <30 CFU / g and a 100% mortality rate for Ascaris eggs, fully meeting safety standards. This demonstrates that the preparation process of this invention has revolutionary advantages in efficiency and product consistency.

[0169] III. Creativity Verification of Features in the Implementation Examples

[0170] For Example 2: An additional experiment was conducted, in which a periodic weak magnetic field was applied to the treatment area of ​​experimental group 2 during the seedling stage. The root development and phosphorus absorption efficiency of the seedlings were compared, and the results showed that the efficiency was increased by 15-20% compared with the case where no magnetic field was applied, thus verifying the enhancing effect of "magnetic guidance" on mycelial network and nutrient targeting.

[0171] For Example 3: Using a closed growth chamber, the net CO2 absorption rate of the matrix in the experimental group 3 under light was monitored, and the amount of calcium carbonate deposition in the core particles was detected to quantitatively verify its dual effect of photosynthetic carbon fixation and mineral strengthening.

[0172] Regarding Example 4: The core bacterial agent prepared in Experimental Group 4 and Experimental Group 1 (laboratory pure culture bacterial agent) were simultaneously inoculated into disease-suppressing soil. The colonization and survival rate of Trichoderma harzianum in the two groups were compared. The survival rate of Trichoderma harzianum in Experimental Group 4 was more than 30% higher, which verifies that the strain cultivated by open fermentation has stronger environmental competitiveness.

[0173] IV. Experimental Conclusions

[0174] Tables 1-4 above irrefutably demonstrate that the present invention (especially Embodiment 1) possesses outstanding inventiveness compared to the prior art from the following four dimensions:

[0175] Revolutionary response mechanism: It leaps from "passive release" that depends on water to "active intelligent response" that senses chemical, physical and biological signals, realizing a dialogue between fertilizer and crops.

[0176] The systematic nature of the functional structure: It has evolved from a chaotic "physical mixture" to a four-dimensional programming collaboration that is precisely executed according to spatiotemporal programs, with each functional module taking over efficiently to form a gain closed loop.

[0177] The holistic nature of soil improvement: evolving from a one-sided exogenous additive to an "ecosystem engineer" that systematically reconstructs the healthy microenvironment of the rhizosphere through physical, chemical, and biological means.

[0178] Advanced technology and raw materials: The process has evolved from extensive composting and simple processing to deep functional modification and rapid stabilization, realizing the high-value utilization of waste resources and the standardization of product quality.

[0179] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

[0180] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An organic fertilizer beneficial to seedling cultivation, characterized in that, The organic fertilizer is a regular particle with a core and four layers. The core and four layers include a multifunctional composite core and a fourth, third, second and first layer that wrap around the multifunctional composite core from the inside out. The multifunctional composite core includes a humified organic carrier with an endogenous hyphal network formed by pre-fermentation and colonization of Trichoderma harzianum, and nutrient slow-release microcapsules dispersed therein; the humified organic carrier includes at least alkali-microwave synergistically modified straw fiber and ultrasonically pretreated seaweed residue. The first layer is a signal response layer, which is composed of a polysaccharide-based gel material that is sensitive to plant root exudates, and contains growth-promoting microorganisms and seed germination stimulants. The second layer is a pH buffer release layer, which is composed of pH-responsive polymer materials and loaded with humic acid-chelated trace elements and phosphorus- and potassium-solubilizing microbial agents. The third layer is a moisture / stress sensing layer, which is composed of a smart gel material that is sensitive to changes in environmental moisture content or salt concentration, and is loaded with osmotic regulating substances and stress-resistant microbial agents. The fourth layer is a biological competition and activation layer, which is composed of a gel material containing microbial quorum sensing inhibitors and specific beneficial microbial carbon sources.

2. The organic fertilizer beneficial to seedling cultivation according to claim 1, characterized in that, The method for preparing the alkali-microwave synergistic modified straw fiber includes: A. Soak the straw in a 2%-3% alkaline solution at 80-85℃ for 1.5-2.5 hours, then rinse until neutral; B. Microwave treatment with a power of 300-500 W for 5-8 minutes; C. Loading with humic acid and drying and pulverizing, the resulting modified straw fiber has a specific surface area ≥15 m². 2 / g.

3. The organic fertilizer beneficial to seedling cultivation according to claim 2, characterized in that, In the step of loading humic acid in the preparation of alkali-microwave modified straw fiber, trace amounts of plant root-specific signaling molecules such as salicylic acid precursors, methyl jasmonate analogs, or chitin oligosaccharides are simultaneously loaded; and in the initial stage of Trichoderma harzianum pre-fermentation colonization, low concentrations of fungal quorum sensing inhibitors such as farnesol are added.

4. The organic fertilizer beneficial to seedling cultivation according to claim 1, characterized in that, During the preparation of the nutrient sustained-release microcapsules, superparamagnetic iron oxide nanoparticles are embedded in the wall material. During the pre-fermentation and colonization process of Trichoderma harzianum, a weak static magnetic field of 5-15 mT with a constant direction is applied to the reaction system.

5. The organic fertilizer beneficial to seedling cultivation according to claim 1, characterized in that, During the preparation of the humified organic carrier, an additional 0.5%-2% of a composite photosensitive material of graphitic carbon nitride and ferric humate is incorporated into the entire humified organic carrier. After the pre-fermentation of Trichoderma harzianum is completed and before the outer coating is applied, a photocatalytic interface construction step is added. The photocatalytic interface is constructed by vapor deposition or spraying on the surface of the multifunctional composite core particles to form an ultrathin titanium dioxide or TiO2 / g-C3N4 heterojunction nanocoating.

6. The organic fertilizer beneficial to seedling cultivation according to claim 1, characterized in that, The pretreatment parameters for the ultrasonically pretreated seaweed residue are as follows: Frequency 25-40 kHz; Power 150-200 W; The processing time is 15-30 min, and the dissolution rate of seaweed polysaccharides after processing is not less than 60%.

7. The organic fertilizer beneficial to seedling cultivation according to claim 1, characterized in that, The first layer is a signal response layer, the polysaccharide-based gel material is a complex of sodium alginate and pectin; the growth-promoting microorganism is a gelatinous Bacillus; and the germination stimulant is alginate oligosaccharide. The second layer is a pH buffer release layer, and the pH-responsive polymer material is a complex of chitosan and humic acid; the trace elements include at least calcium, magnesium, iron, and zinc humic acid chelates; the phosphorus-solubilizing and potassium-solubilizing microbial agents include Pseudomonas fluorescens; The third layer is a moisture / stress sensing layer. The smart gel material contains a thermosensitive polymer, poly(N-isopropylacrylamide), and / or a salt-sensitive polymer. The osmotic regulator includes proline or betaine. The stress-resistant microbial agent is a Bacillus subtilis strain with drought-resistant or salt-tolerant functions. The fourth layer is a biological competition and activation layer, with furanone or its derivatives serving as the microbial quorum sensing inhibitor; and chitin oligosaccharides or chitosan oligosaccharides serving as the carbon source for specific beneficial microorganisms.

8. The organic fertilizer beneficial to seedling cultivation according to claim 1, characterized in that, In the preparation of the humified organic carrier, photosynthetic cyanobacteria such as Synechococcus are co-inoculated with Trichoderma harzianum, and a special hydrogel such as a printable gel is selected as the common substrate for the hyphae and cyanobacteria. The cyanobacteria use light, water and CO2 in the air to perform photosynthesis, producing oxygen and organic carbon.

9. The organic fertilizer beneficial to seedling cultivation according to claim 1, characterized in that, In the aforementioned Trichoderma harzianum pre-fermentation colonization, the mixture of amino acids produced by the acid hydrolysis of diseased and dead livestock and poultry is used to adjust the pH value of the fermentation material, creating a selective fermentation environment that is only beneficial to Trichoderma harzianum and inhibits most other bacteria.

10. A method for preparing an organic fertilizer beneficial to seedling cultivation according to any one of claims 1-9, characterized in that, Includes the following steps: Step 1: Deep pretreatment of raw materials Preparation of alkali-microwave synergistic modified straw fiber, ultrasonically pretreated seaweed residue and fully decomposed high-nitrogen organic material. Step 2: Constructing the core of the endophytic hyphal network The modified straw fiber, seaweed residue, well-rotted high-nitrogen organic material, mineral skeleton material, and humic acid obtained in step one are mixed and inoculated with Trichoderma harzianum for solid-state fermentation, allowing the mycelium to grow and colonize fully inside the carrier to form an endogenous network. After fermentation, the mixture is mixed with nutrient slow-release microcapsules and granulated to obtain multifunctional composite core particles. The solid-state fermentation conditions are a temperature of 25-30℃, a humidity of 55-65%, and a fermentation time of 5-7 days. Step 3: Preparation of coating solution Four functional coating solutions were prepared for forming the fourth, third, second, and first layers, respectively. Step 4: Gradient Coating In the fluidized bed coating equipment, the multifunctional composite core particles obtained in step two are sequentially coated with the fourth, third, second and first layers of spray coating, and each layer is dried and cured at a suitable temperature after coating. Step 5: Post-ripening and Finished Product Processing The coated granules are matured under controlled temperature and humidity conditions, then dried to the specified moisture content, and sieved to obtain the finished product.