Herbicide-resistant rice OsACC gene promoter mutant and application thereof

By deleting a portion of the nucleic acid sequence from the promoter of the rice OsACC gene and using the CRISPR/Cas12i3 system, a rice OsACC gene mutant was constructed. This solved the problems of growth and development defects and weed resistance evolution caused by ACCase inhibitor herbicides in rice in the existing technology, and achieved resistance to quizalofop-p-ethyl and clethodim, thus improving the efficiency of herbicide use and the safety of agricultural production.

CN121950855APending Publication Date: 2026-05-01INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Patent Information

Application Number
CN202610432585.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-03
Publication Date
2026-05-01

AI Technical Summary

Technical Problem

In the existing technology, the frequent use of a single herbicide leads to the evolution of weed resistance and phytotoxicity problems. Furthermore, the mutations of existing rice ACCase inhibitor herbicides often cause growth and development defects, limiting their application in agricultural production.

Method used

By deleting a portion of the nucleic acid sequence from the promoter of the rice OsACC gene, and using the CRISPR/Cas12i3 system or other gene editing technologies, rice OsACC gene mutants can be constructed to achieve resistance to ACCase inhibitor herbicides. Specific methods include constructing the pHZLib-Cas12i3 vector and infecting rice callus tissue to screen for resistant plants.

Benefits of technology

Rice varieties resistant to quizalofop-P-ethyl and clethodim were obtained, ensuring agricultural production safety, improving herbicide application efficiency, and avoiding growth and development defects.

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Abstract

The invention relates to a herbicide-resistant rice OsACC gene promoter mutant and application thereof. The rice OsACC gene mutant is a deletion mutant; wherein the deletion mutation occurs in the promoter of the rice OsACC gene, and the sequence of the nucleic acid before the deletion mutation is shown as SEQ ID No.1; the length of the sequence of the deleted nucleic acid is 14 bp to 30 bp, A in the initiation codon ATG of the OsACC gene is 0 site, the deleted nucleic acid occurs in 964 to 935 sites on the upstream of the initiation codon ATG, and at least bases on 958 to 945 sites on the upstream of the initiation codon ATG are deleted. The mutant can enable rice to obtain resistance to herbicide fluazifop-p-butyl and / or clethodim.
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