Comamonas, compound microbial agent and application thereof

Abstract

The invention relates to the technical field of agricultural microorganisms. The invention discloses a strain of Comamonas odontotermes GZL 91, and the collection number of the Comamonas odontotermes GZL 91 is CGMCC (China General Microbiological Culture Collection Center) NO.36852. The invention further discloses a preparation method of the Comamonas odontotermes GZL 91. The strain GZL 91 is preserved in the China General Microbiological Culture Collection Center on November 27th, 2025, the Chinese name of the strain GZL 91 is called comamonas, the Latin name of the strain GZL 91 is Comamonas odontobacteria, the preservation number of the strain GZL 91 is CGMCC NO.36852, the preservation center is called CGMCC for short, and the address of the preservation center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of the strain GZL 91 is as shown in SEQ ID NO.1 in a sequence table. The composite microbial agent containing the strain is used for straw in-situ decomposition, the early-stage decomposition effect is remarkable, straw is powdery, the physicochemical property of soil can be remarkably improved, and the content of organic matter in the soil is increased by 15%; and the crop yield is increased by about 10%.

Description

Technical Field

[0001] This invention belongs to the field of agricultural microbiology technology. Background Technology

[0002] The black soil in Northeast my country is characterized by high productivity and high natural fertility. However, due to over-cultivation and unreasonable utilization in recent years, coupled with adverse environmental factors such as wind erosion, water erosion, and freeze-thaw erosion, the black soil in Northeast my country has not only experienced a decline in soil organic matter content, but the black soil layer has also gradually thinned.

[0003] Conservation tillage primarily reduces soil disturbance by returning crop straw to the field, optimizes soil structure, promotes the formation of organic matter, and ensures its long-term stability, thereby guaranteeing good physical and chemical properties and high productivity. On the other hand, implementing conservation tillage techniques in the Northeast black soil region can also effectively utilize crop straw, addressing the problem of straw surplus and environmental pollution caused by straw burning in Northeast China, while simultaneously enriching the soil.

[0004] However, the complex structure of straw and the consistently low temperatures in Northeast China limit the ability of soil microorganisms to degrade the lignocellulose in straw. Currently, most commercially available microbial agents suffer from limited functionality. Therefore, developing a composite functional microbial agent with clearly defined microbial members, capable of efficiently and in-situ decomposing straw under low-temperature conditions, and possessing both biocontrol and environmental remediation capabilities, is a pressing issue in protecting black soil resources and promoting in-situ straw return to fields in Northeast China. Summary of the Invention

[0005] In view of this, and addressing the problems of low straw decomposition efficiency, inefficient utilization of straw resources, and soil degradation under low-temperature conditions, this invention aims to provide a strain of *Comamonas odontotermitis* GZL 91, with the accession number CGMCC NO.36852. This strain GZL 91 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC). The Chinese name of strain GZL 91 is *Comamonas odontotermitis*, and its Latin name is *Comamonas odontotermitis*. The accession number for strain GZL 91 is CGMCC NO.36852. The CGMCC is located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of strain GZL 91 is shown in SEQ ID NO.1 of the sequence listing.

[0006] This invention also provides a compound microbial agent for promoting in-situ decomposition of straw, containing *Comamonas odontotermitis* GZL91, *Pedobacter rhizosphaerae* GZL93, *Paenibacillus illinoisensis* ARQ82, and *Paenibacillus xylanexedens* ARQ86-1. Strain GZL93 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC). The Chinese name of strain GZL93 is *Pedobacter rhizosphaerae*, and its Latin name is *Pedobacter rhizosphaerae*. The accession number for strain GZL93 is CGMCC NO.36851. The abbreviation of the collection center is CGMCC, and its address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of strain GZL93 is shown in SEQ ID NO.2 of the sequence listing. Strain ARQ82 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC). The Chinese name of strain ARQ82 is *Paenibacillus illinoisensis*, and its Latin name is CGMCC NO.36790. The CGMCC is located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of strain ARQ82 is shown in SEQ ID NO.3 of the sequence listing. Strain ARQ86-1 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC). The Chinese name of strain ARQ86-1 is *Paenibacillus xylanexedens*, and its Latin name is CGMCC NO.36853. The abbreviation of the collection center is CGMCC, and its address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of strain ARQ86-1 is shown in SEQ ID NO.4 of the sequence listing.

[0007] The strain of this invention has good low-temperature resistance and regional adaptability, and also improves the physical and chemical properties of soil, with the organic matter content increasing by more than 15% year-on-year. Attached Figure Description

[0008] Figure 1 The cellulase activity of a single bacterium after 7 days of low-temperature culture.

[0009] Figure 2The activity of single-strain hemicellulase after 7 days of low-temperature culture.

[0010] Figure 3 The activity of laccase in a single bacterium after 7 days of culture at medium and low temperatures.

[0011] Figure 4 The decomposition rate of single-strain straw after 7 days of medium-low temperature culture.

[0012] Figure 5 These are morphological diagrams of the various strains in the compound microbial inoculant.

[0013] Figure 6 The assay is a determination of lignocellulase activity after 15 days of liquid culture with a compound microbial agent.

[0014] Figure 7 The determination of straw decomposition rate by compound microbial inoculants cultured in liquid at different temperatures for 15 days.

[0015] Figure 8 These are scanning electron microscope images of various structures in straw cultured in liquid for 15 days.

[0016] Among them, A, B, and C correspond to the straw lignin outer shell, longitudinal section, and cross section of the blank control group, respectively.

[0017] A', B', and C' correspond to the outer shell, longitudinal section, and cross section of the straw lignin inoculant, respectively.

[0018] Figure 9 The determination of straw decomposition rate in a pot experiment at different temperatures for 30 days using compound microbial inoculants.

[0019] Figure 10 This is the result of measuring the decomposition rate of rice straw.

[0020] Figure 11 This is the result of measuring the decomposition rate of corn stalks.

[0021] Figure 12 The results show the changes in straw morphology after 45 days of quantitative field testing with compound microbial agents.

[0022] Wherein, a represents the morphology of rice straw in the blank control group; a' represents the morphology of rice straw in the CN experimental group; b represents the morphology of corn straw in the blank control group; and b' represents the morphology of corn straw in the CN experimental group.

[0023] Figure 13 This study investigated the effect of in-situ straw return to the field on soil organic matter content using a quantitative experiment. Detailed Implementation

[0024] The present invention will be further described below with reference to embodiments. These embodiments are illustrative and not limiting, and should not be construed as limiting the scope of protection of the present invention. Unless otherwise specified, the methods used in this invention are conventional methods in the art.

[0025] Example 1

[0026] Construction of in-situ decomposition functional microbiome and morphological identification of core degrading strains

[0027] 1. Soil sample collection and bacterial strain isolation and screening

[0028] Soil samples for this invention were collected from Arong Banner and Gongzhuling areas. 10g of each sample was weighed and placed in conical flasks containing glass beads and an appropriate amount of sterile physiological saline. After shaking and resuscitation for 3 hours, the samples were allowed to stand overnight. The soil suspension was then added to an enrichment medium and cultured at 25℃ and 100rpm with shaking. Each generation lasted 5 days, and the culture was repeated up to 20 generations. This yielded a relatively stable natural decomposing microbial community under low-temperature conditions.

[0029] Soil microbial communities were diluted to 10 using a combination of gradient dilution and extinction dilution. -8 10 -9 10 -10 Take 100 μL of diluted bacterial solution and spread it onto the screening medium. Incubate upside down at room temperature for 3-5 days until single colonies grow in the medium.

[0030] The bacteria were picked and streaked for purification until single colonies with consistent morphology grew. Finally, 12 strains were screened from the natural decomposing bacteria.

[0031] 2. Screening of single bacteria with in situ decomposition function

[0032] The enzyme activities of lignocellulose-related degrading enzymes and the straw decomposition rate of the 12 selected single strains were determined. Figure 1-3 As shown, the dominant strains that play a key role in degradation function were identified.

[0033] All strains had cellulase activity above 0.03 U / mL, with strain GZL 91 having the highest cellulase activity at 0.22 U / mL, making it the dominant strain for cellulose degradation.

[0034] In terms of hemicellulase activity, strains ARQT 81, ARQT 82, ARQ 82, and ARQ86-1 all exhibited enzyme activities exceeding 0.15 U / mL, making them suitable as potential superior strains for hemicellulase degradation. Among them, strain ARQ 86-1 showed the highest hemicellulase activity at 0.70 U / mL, demonstrating absolute dominance in hemicellulase degradation.

[0035] All single strains exhibited laccase activity above 0.14 U / mL, but strain GZL 93 showed stronger laccase activity at 1.19 U / mL, giving it an absolute advantage.

[0036] In the single-strain decomposition rate determination experiment, under 15 ℃ conditions, ARQ 82 and ARQ 86-1 were cultured under oligotrophic conditions for 7 days, and the straw decomposition rate reached 35.67% and 33% respectively. This shows that these two strains have strong decomposition function, which may be related to the high enzyme activities of cellulase and hemicellulase in both strains.

[0037] 3. Construction of in-situ decomposition functional compound microbial agents

[0038] The enzyme activities of lignocellulose-related degrading enzymes and the straw decomposition rate of 12 single-strain members were determined. Four key degrading strains were obtained to form a compound microbial inoculum: strain GZL 91 (dominant in cellulose degradation); strain ARQ 86-1 (dominant in hemicellulose degradation); strain GZL 93 (dominant in lignin degradation); and strain ARQ 82 (dominant in single-strain decomposition under medium- and low-temperature conditions). The morphology of the strains is as follows: Figure 5 As shown.

[0039] A bottom-up construction approach was adopted to recombine strains with different degradation advantages through functional complementarity, resulting in a composite microbial agent containing both strains with high lignocellulose-degrading enzyme activity and strains with optimal single-strain decomposition effects. Molecular biological identification and degradation function verification were performed on the individual strains within the composite microbial agent. The advantageous strains with complementary lignocellulose-related degradation functions were recombinated, and the strains were observed under an electron microscope.

[0040] Strain GZL 91 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC). The Chinese name of strain GZL 91 is *Comamonas odontotermitis*, and its Latin name is CGMCC NO.36852. The CGMCC is located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of strain GZL 91 is shown in SEQ ID NO.1 of the sequence listing.

[0041] Strain GZL93 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC). The Chinese name of strain GZL93 is *Pedobacter rhizosphaerae*, and its Latin name is *Pedobacter rhizosphaerae*. The accession number for strain GZL93 is CGMCC NO. 36851. The abbreviation of the collection center is CGMCC, and its address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of strain GZL93 is shown in SEQ ID NO. 2 of the sequence listing.

[0042] Strain ARQ82 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC). The Chinese name of strain ARQ82 is *Paenibacillus illinoisensis*, and its Latin name is CGMCC NO.36790. The CGMCC is located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of strain ARQ82 is shown in SEQ ID NO.3 of the sequence listing.

[0043] Strain ARQ 86-1 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC). The Chinese name of strain ARQ 86-1 is *Paenibacillus xylanexedens*, and its Latin name is CGMCC NO.36853. The CGMCC is located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of strain ARQ 86-1 is shown in SEQ ID NO.4 of the sequence listing.

[0044] Example 2

[0045] Validation of Lignocellulose Degradation Function

[0046] The compound microbial agent was cultured in liquid shake flasks under room temperature, medium-low temperature, and low temperature conditions. Five ml of the culture solution after 15 days of liquid culture at 4 ℃, 15 ℃, and 25 ℃ was transferred to sterile centrifuge tubes and centrifuged at 12000 rpm for 2 min. The supernatant was the crude enzyme solution. The activities of cellulase, hemicellulase, and laccase in the compound microbial agent were determined. The results are as follows: Figure 6 The results showed that, under medium and low temperature culture conditions, the cellulase and hemicellulase activities of the compound microbial agent CN after 15 days exhibited strong lignin degradation ability.

[0047] Table 1. Determination of lignocellulase activity after 15 days of microbiome liquid culture

[0048]

[0049] Note: CN stands for compound microbial inoculant.

[0050] Example 3

[0051] Straw decomposition function verification

[0052] 1. Validation of straw decomposition function in liquid culture

[0053] After determining the activity and growth of lignocellulase in the compound microbial inoculant, the remaining fermentation broth and straw fragments in the conical flask were poured into a gauze bag labeled with sample numbers. The bacterial broth was filtered out, and large, relatively intact, undecomposed straw fragments were picked out. These fragments were rinsed several times with distilled water to remove surface bacteria. After drying at 60 ℃ for 2–3 days, the straw fragments were weighed using the loss-on-weight method, and the straw decomposition rate was calculated: Straw decomposition rate = (W0 - W1) / W0 * 100% (W0: original dry weight of straw; W1: dry weight of decomposed large straw fragments). The straw decomposition status of the microbiome after 15 days of liquid culture under different temperature conditions was obtained. Figure 7 As shown in Table 2, the decomposition effect of the compound microbial inoculant CN was significantly better than that of the blank control group, with a straw decomposition rate of 47.07%. The degradation rates of the compound microbial inoculant CN at 4 ℃ and 15 ℃ reached 25.67% and 41%, respectively, which were 19.00% and 31.70% higher than those of the control group. This demonstrates that the compound microbial inoculant possesses low-temperature resistance and high-efficiency decomposition capabilities. The microstructure of the straw samples after liquid culture was observed using scanning electron microscopy (SEM). Figure 8 As shown, the compound microbial inoculant significantly altered the lignin outer shell, longitudinal section, and cross-sectional morphology of straw compared to the control group. In the control group, the straw structure was dense and regularly arranged, with no obvious microstructural decay. However, the straw treated with the compound microbial inoculant exhibited large areas of irregular decay in its longitudinal and cross-sectional sections, with the arrangement no longer regular and orderly, and obvious decay pores appearing in the recalcitrant lignin outer shell.

[0054] Table 2. Determination of straw decomposition rate in microbial communities after 15 days of liquid culture at different temperatures

[0055]

[0056] Note: CK means no bacterial agent added, CN means compound microbial agent added.

[0057] 2. Simulation test verification of straw turning and returning to the field

[0058] The cultured microbial inoculum was thoroughly mixed with corn stalk fragments, soaked in a self-sealing bag for 30 minutes, and then mixed evenly with pre-prepared garden soil in a potted culture box. The mixture was placed under different temperature conditions: 15 ℃ (temperature incubator) and 4 ℃ (indoor environmental chamber). Each treatment group had three replicates, and the experiment lasted 30 days. During the experiment, sterile water was added regularly to maintain soil moisture. After the experiment, the soil from each treatment group was sieved through a 4-mesh sieve to remove undecomposed straw. The removed straw was then placed on a double layer of 100-mesh (0.15 mm) sieve to ensure no straw was lost. The straw was rinsed with running water to remove adhering soil and microbial residues. This rinsing was repeated several times. The cleaned straw was then dried in a 68 ℃ oven, and the straw decomposition rate was determined using the loss-in-weight method. Figure 9 As shown in Table 3, after a 30-day simulated test of straw incorporation into the field, the decomposition rate of the compound microbial agent reached 40.5% and 56.3% at 4℃ and 15℃, respectively, demonstrating good decomposition effects.

[0059] Table 3. Determination of straw decomposition rate in pot experiments with compound microbial inoculants at different temperatures for 30 days

[0060]

[0061] Note: CK represents no bacterial agent (blank control), CN represents compound microbial agent.

[0062] 3. Soil testing in the experiment of incorporating soil into the field

[0063] The compound microbial inoculant was tested in a pot experiment to simulate a field environment. Soil samples were taken after the compound microbial inoculant was incorporated into the field, and 16S rRNA amplicon sequencing was performed to analyze changes in soil microbial community structure and diversity after inoculation. The results showed that after inoculation with the compound microbial inoculant, the species richness and diversity of the microbial community decreased, while the community homogeneity increased. The community composition was more concentrated in the compound microbial inoculant and certain key degradation species in the soil. The inoculation of the compound microbial inoculant, to some extent, disturbed the composition of the soil microbial community structure.

[0064] Example 4

[0065] Quantitative test on the in-situ decomposition performance of straw

[0066] 1. Preparation of microbial inoculants

[0067] The glycerol-preserved microbial inoculant, stored at -80℃, was streaked onto fresh LB agar plates for activation. After colony growth, it was transferred to fresh LB liquid medium and cultured at 30℃ and 220 rpm for 24 h using a shaker. The seed culture was then collected. A 10% inoculum was added to a 5 L small fermenter containing fermentation medium. Defoaming and feeding were performed promptly. Once the bacteria reached the plateau phase (OD approximately 30-50), the fermenter was immediately removed from the tank, the fermentation broth was collected, balanced, and dispensed into centrifuge bottles. The bacterial precipitate was collected after centrifugation at 5000 rpm for 25 min using a large centrifuge and stored in an ice box. The precipitate was then transported to a demonstration area for field trials on the same day. Before use, an appropriate amount of water was added to resuspend the bacterial precipitate, and the mixture was thoroughly mixed to obtain the inoculant to be applied. Commercial inoculants were diluted according to the instructions for use.

[0068] 2. Quantitative test of in-situ corrosion of PVC pipe

[0069] Two treatment groups were set up: a blank control group without microbial agent application and an experimental group with compound microbial agent application. Each group had 12 flat-hole PVC pipes. 34 g of rice straw was weighed and mixed thoroughly with 170 mL of microbial agent, then mixed with 3.9 kg of soil sample and placed into the PVC pipes. A mesh bag was then placed over the pipes and positioned in the field. In this experiment, the amount of straw, the microbial content in the mesh bag (1.3 × 10⁶ CFU / g straw), and the soil sample were all quantitative, allowing for more accurate determination of the in-situ decomposition performance of rice straw. Starting in June each year, at least three samplings were required before the rice autumn harvest. Every month, straw from three parallel PVC pipes or mesh bags in each treatment group was collected to determine the decomposition rate. Simultaneously, soil samples were collected from each parallel using a five-point sampling method. Straw and soil samples from the middle layer of the PVC pipes were selected for microbiological analysis and soil physicochemical property testing. Figure 10 As shown in Table 4, the decomposition rate of rice straw treated with the compound microbial agent reached about 50% in the early stage, which was more than 20% higher than that of the blank control group, showing a significant effect in the early stage.

[0070] Table 4. Measurement of rice straw decomposition rate

[0071]

[0072] Note: CK represents no bacterial agent (blank control), CN represents compound microbial agent.

[0073] 3. Quantitative test of in-situ decomposition of mesh bags

[0074] A quantitative experiment on the in-situ decomposition of corn stalks in mesh bags was conducted, with two treatment groups: a blank control group without microbial agents and an experimental group treated with a compound microbial agent. Mesh bags of 40×60 cm were used. 40 g of dry corn stalks were mixed with 200 mL of the experimental microbial agent and soaked for 30 min. This mixture was then thoroughly mixed with a quantitative soil sample and placed in the mesh bag, corresponding to the type of return to the field in the cornfield. Sampling was conducted annually starting in June and completed at least three times before the autumn harvest. Every month, three parallel mesh bags from each treatment group were used to determine the decomposition rate of the stalks. Figure 11 As shown in Table 5. Simultaneously, soil samples were collected at five points in each parallel experiment, and soil samples from the middle layer of the corn stalks in the mesh bags were selected for microbiological analysis and soil physicochemical property testing. In the in-situ quantitative experiment of corn stalks in mesh bags, the decomposition rate of corn stalks in the two experimental groups inoculated with compound microbial agents was more than 20% higher than that in the initial stage of the blank control group. The decomposition rate of corn stalks during the autumn harvest could reach over 85%, without affecting the following year's cultivation.

[0075] Table 5. Measurement of Corn Stalk Decomposition Rate

[0076]

[0077] Note: CK represents no bacterial agent (blank control), CN represents compound microbial agent.

[0078] Example 5

[0079] Straw in-situ return to field plot experiment

[0080] A small-plot experiment on the application of microbial agents was conducted before the growing season of corn / rice. Three treatment groups were set up: a control group without microbial agents, an experimental group with compound microbial agents, and a control group. The area and amount of microbial agents applied in each treatment group were kept consistent. No additional organic fertilizer was applied, and the viable count of the microbial agents was guaranteed to be no less than 2 × 10⁻⁶. 11 CFU / acre. For example... Figure 12 As shown.

[0081] Example 6

[0082] Field performance evaluation of decomposing microbiome

[0083] 1. Determination of straw decomposition rate in field trials

[0084] All the straw in the mesh bags was removed through a sieve, and the surface soil and bacteria were repeatedly washed away with running water. After drying in an oven, the straw was weighed, and the decomposition rate was calculated. The decomposition rate formula is as follows: Straw decomposition rate = (W0 - W1) / W0 * 100% (W0: original dry weight of straw; W1: dry weight of straw after decomposition). Overall, after the compound microbial agent was introduced, the decomposition effect of straw in each demonstration area was better in the early stage of straw decomposition, and the straw decomposition speed was faster. The initial straw decomposition rate could approach 60%, which is more than 15% higher than the decomposition rate of the blank control group.

[0085] 2. Determination of soil organic carbon content

[0086] The organic carbon content in soil samples was determined using the potassium dichromate oxidation-spectrophotometric method. After drying, the soil sample was passed through a 0.25 mm sieve. A certain amount of soil sample was weighed into a test tube, and 10 mL of 0.4 mol / L potassium dichromate-sulfuric acid solution was added and shaken well. A glass funnel was placed at the mouth of the test tube, which was then inserted into an iron wire furnace and placed in oil at 170-180℃. The solution in the test tube was allowed to boil for 5 minutes. After cooling, the liquid and soil sample in the test tube were transferred to an Erlenmeyer flask, along with the washing solution used to clean the test tube and funnel, to a final volume of 50-60 mL. Three drops of o-phenanthroline indicator were added, and the remaining potassium dichromate was titrated with ferrous sulfate solution. Approximately 0.2 g of ignited pumice powder or soil was used as a blank test. The formula for calculating soil organic carbon is as follows:

[0087] OM=[c×(V0-V)×0.003×1.10 / m]*1000

[0088] In the formula, OM is the mass fraction of soil organic carbon (g / kg); c is the concentration of ferrous sulfate standard solution (mol / L); V0 is the volume of ferrous sulfate standard solution consumed in the blank test (mL); V is the volume of ferrous sulfate standard solution consumed in the sample test (mL); 0.003 is the millimolecular mass of 1 / 4 carbon atom (g); 1.10 is the oxidation correction coefficient; m is the mass of the dried sample (g); and 1000 is the coefficient for converting the result to content per kilogram. The results of parallel determinations are expressed as the arithmetic mean, retaining three significant figures. Figure 13 As shown in Table 6, the quantitative experiment on in-situ straw return to the field generally improved the soil's physical and chemical properties to some extent.

[0089] Table 6. Effects of quantitative straw in-situ return experiment on soil organic matter content

[0090]

[0091] Note: CK represents no bacterial agent (blank control), CN represents compound microbial agent.

Claims

1. Commonas odontotermitis GZL 91, with accession number CGMCC NO. 36852.

2. A compound microbial agent containing *Comamonas sodontotermitis* GZL91 as described in claim 1, and also containing *Pedobacter rhizosphaerae* GZL93, *Paenibacillus illinoisensis* ARQ82 and / or *Paenibacillus xylanexedens* ARQ86-1.

3. The application of the *Comamonas odontotermitis* GZL 91 as described in claim 1 or the compound microbial agent as described in claim 2 in the decomposition of high cellulose.

4. The application of the *Comamonas odontotermitis* GZL 91 as described in claim 1 or the compound microbial agent as described in claim 2 in the decomposition of high hemicellulose.

5. The application of the *Comamonas odontotermitis* GZL 91 as described in claim 1 or the compound microbial agent as described in claim 2 in the decomposition of high-lignin compounds.

6. The application of the *Comamonas odontotermitis* GZL 91 as described in claim 1 or the compound microbial agent as described in claim 2 in the in-situ decomposition of straw.

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