Insecticidal composition containing entomopathogenic virus and methyl jasmonate or jasmonic acid

Abstract

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

Description

Technical Field

[0001] This invention relates to the field of biological insecticide technology, and more particularly to an insecticidal composition comprising an insect pathogen virus and methyl jasmonate or jasmonic acid. Background Technology

[0002] Lepidopteran pests, such as the cotton bollworm (Helicoverpa armigera), beet armyworm (Spodopteraexigua), and fall armyworm (Spodoptera frugiperda), are significant biological threats to global agricultural production. The larvae of these pests directly feed on plant leaves, disrupting the photosynthetic system and hindering crop biomass accumulation; studies show that the fresh weight of affected plants can decrease by 37%–62%. Furthermore, insect holes and frass contamination cause a 15%–40% loss in the marketability of agricultural products, and feeding wounds can increase the probability of pathogen infection by 2.8 times, easily triggering complex disasters. Therefore, their control has become a key issue in ensuring sustainable agricultural development.

[0003] Currently, while the control of lepidopteran pests is showing a diversified trend, chemical pesticides still dominate, accounting for approximately 65% ​​of the global market share. Under long-term, high-intensity selective pressure, pesticide resistance in lepidopteran pests is increasing exponentially—for example, the diamondback moth's resistance to pyrethroid insecticides has exceeded 5000 times. More seriously, in 2022, random sampling of agricultural product residues in my country showed that the exceedance rate of standards in vegetables reached 8.7%, an increase of 3.2 percentage points compared to 2015, highlighting the ecological risks of chemical control. Therefore, the development of novel, highly targeted, and environmentally friendly biological agents has become an urgent need for the industry.

[0004] Baculoviruses, as obligate arthropod parasites, are widely considered an ideal alternative to chemical pesticides. Baculoviruses are double-stranded DNA viruses that specifically parasitize arthropods, named for their rod-shaped viral particles. These viruses possess advantages such as strong host specificity, high pathogenicity, safety to non-target organisms, good environmental compatibility, recyclability, and low likelihood of inducing pesticide resistance. They have been recommended by the Food and Agriculture Organization of the United Nations as biopesticides with significant application potential. Baculoviruses can not only suppress insect populations by reducing their reproductive capacity and lifespan and through vertical transmission within the population; they can also spread through the liquefaction of dead insect bodies, spreading the virus in the environment and creating a cycle of infection when ingested by the next generation of pests, achieving a continuous control effect of "one application, multiple generations of control." Furthermore, baculoviruses require live insects or cell culture for propagation, and their production does not require chemically synthesized raw materials, avoiding the "three wastes" problems of chemical pesticide production and meeting the requirements of clean and low-carbon production. Therefore, baculoviruses have become one of the hot topics in global biopesticide research and development. For example, the United States has registered 32 baculovirus insecticide products (EPA, www.epa.gov, 2024), and Canada has registered 16 (PMRA, www.canada.ca, 2024). In China, baculovirus insecticides have also become a key development direction due to their high alignment with the needs of green agriculture. Currently, the number of registered products is nearly 80 (China Pesticide Information Network, http: / / www.icama.org.cn / ), and it is showing a year-on-year growth trend. Among them, the registration growth rate in China in the past five years reached 18.7%, significantly higher than the global average (9.4%). However, compared with chemical insecticides, the relatively low insecticidal activity and slow insecticidal speed have limited the development of baculovirus insecticides. For example, in 2023, my country's production of viral insecticide formulations was about 2,600 tons, accounting for only about 2.3% of the insecticide market share, which is significantly low.

[0005] Jasmonic acid (JA) and its derivative methyl jasmonate (MeJA) are endogenous plant hormones that can induce plants to initiate both direct and indirect defenses. When plants are attacked by adverse environmental factors such as pests, the content of JA (MeJA) in the plant increases significantly, thereby inducing the plant to defend itself. Studies have shown that exogenous MeJA can induce insect-resistant activities in different plants. Therefore, MeJA (JA) is recognized as a novel endogenous plant growth regulator that plays an important role in plant growth and resistance to pests and diseases. To date, no publicly reported technologies or products have been developed for the combined use of jasmonic acid or methyl jasmonate with insect baculoviruses to synergistically control lepidopteran pests. Summary of the Invention

[0006] To overcome the above shortcomings, the present invention aims to provide an insecticidal composition comprising an insect pathogen virus and methyl jasmonate or jasmonic acid, which has a synergistic effect on lepidopteran pests and can enhance the virulence and field control efficacy of the insect pathogen virus.

[0007] Another object of the present invention is to provide a method for controlling lepidopteran pests.

[0008] Another object of the present invention is to provide the use of insecticidal compositions in the control of lepidopteran pests.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides an insecticidal composition comprising an insect pathogen virus and methyl jasmonate or jasmonic acid as active ingredients.

[0010] As a preferred embodiment, the insect pathogenic virus is a baculoviridae virus.

[0011] As a preferred embodiment, the baculoviridae virus is a nucleopolyhedrovirus.

[0012] As a preferred embodiment, the nucleopolyhedrovirus is selected from bollworm nucleopolyhedrovirus, beet armyworm nucleopolyhedrovirus, or cabbage armyworm nucleopolyhedrovirus.

[0013] As a preferred embodiment, the mass ratio of the insect pathogen virus to methyl jasmonate or jasmonic acid is 1:(0.5-1000).

[0014] As a preferred embodiment, the mass ratio is 1:(5 to 500).

[0015] As a preferred embodiment, the composition further comprises a pesticide-acceptable carrier and adjuvants, and is formulated as a suspension concentrate, emulsion, water-dispersible granule, or wettable powder.

[0016] Secondly, the present invention also provides a method for controlling lepidopteran pests, which involves applying methyl jasmonate or jasmonic acid in combination with an insect baculovirus to the target pest or its living environment. The combined application is to apply the insect pathogen virus and methyl jasmonate or jasmonic acid simultaneously, or to apply the insect pathogen virus first, and then apply the methyl jasmonate or jasmonic acid after an interval of 1 to 7 days.

[0017] As a preferred embodiment, the lepidopteran pest is the cotton bollworm, beet armyworm, or fall armyworm.

[0018] Thirdly, the present invention also provides the use of the above-mentioned insecticidal composition in the control of lepidopteran pests.

[0019] According to the above technical solution, the beneficial effects of the present invention are: 1. The synergistic composition containing entomopathogenic viruses and methyl jasmonate or jasmonic acid provided by the present invention exhibits a clear synergistic effect on lepidopteran pests under specific ratios. Compared with the use of entomopathogenic viruses alone, this composition can significantly reduce the amount of food consumed by pests and accelerate the rate of death, thereby greatly improving the field control effect and having significant economic benefits.

[0020] 2. The active ingredients used in the composition provided by this invention are all biological substances with good environmental compatibility. They are not easy to remain in crops and the environment, which helps to delay the development of pesticide resistance in pests, while ensuring the quality and safety of agricultural products and ecological safety, and also meets the requirements of sustainable development of green agriculture.

[0021] 3. The composition provided by this invention is friendly to non-target organisms, conforms to the concept of green prevention and control, is suitable for promotion and application in modern green prevention and control systems, and has good market prospects and social benefits. Attached Figure Description

[0022] Figure 1 This is a schematic diagram showing the cross-distribution of chili seedlings from different experimental treatment groups.

[0023] Figure 2 This is a comparative image showing the selective feeding of beet armyworm larvae on chili seedlings sprayed with SeMNPV and SeMNPV+MeJA, respectively. In the image, the left side shows chili seedlings treated with SeMNPV, and the right side shows chili seedlings treated with SeMNPV+MeJA. Figure 3 The figure shows the significance of differences in tobacco feeding area after MbMNPV-CHN1 and MeJA mixture treatment. Different letters in the figure indicate significant differences between treatments (P < 0.05), and the same letter indicates no significant differences between treatments (P > 0.05). Detailed Implementation

[0024] The following embodiments further illustrate the content of the present invention, but should not be construed as limiting the present invention. Any modifications or substitutions made to the methods, steps or conditions of the present invention without departing from the spirit and substance of the present invention shall fall within the scope of the present invention.

[0025] The composition containing insect pathogen virus and methyl jasmonate or jasmonic acid described in this invention is illustrated by the following examples, but does not limit the scope of the invention.

[0026] The insecticidal composition provided by this invention comprises an insect pathogen virus and methyl jasmonic acid or jasmonic acid as its active ingredients. The insect pathogen virus is preferably a baculoviridae virus, more preferably a nucleopolyhedrovirus (NPV), such as cotton bollworm nucleopolyhedrovirus (HearNPV), beet armyworm nucleopolyhedrovirus (SeMNPV), or cabbage armyworm nucleopolyhedrovirus (MbMNPV). The mass ratio of the insect pathogen virus to methyl jasmonic acid or jasmonic acid can be 1:0.5 to 1000, preferably 1:5 to 500.

[0027] The composition may also contain pesticide-acceptable carriers and adjuvants to formulate a suitable formulation for agricultural use, such as suspension concentrates, emulsions, water-dispersible granules, or wettable powders. The carriers and adjuvants include, but are not limited to: Solvents: such as ethanol, methanol, phosphate buffer, etc.

[0028] Emulsifiers: such as Tween, Emulsifier 656H, Agricultural Emulsion 500, Calcium Dodecylbenzene Sulfonate, Alkylphenol Formaldehyde Resin Polyoxyethylene Ether, Polyoxyethylene Stearyl Ether, NNO, NP, etc.

[0029] Dispersants: such as sodium lignosulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, naphthalene sulfonic acid formaldehyde condensate, arylphenol polyoxyethylene succinate sulfonate, bis(alkyl)naphthalene sulfonate formaldehyde condensate, fatty acid polyoxyethylene ester, octylphenol polyoxyethylene ether sulfate, etc.

[0030] Wetting agents: such as sodium dodecyl sulfate, silkworm excrement, sorbitol fatty acid ester polyoxyethylene ether, sodium dodecylbenzene sulfonate, etc.

[0031] Disintegrants: such as bentonite, citric acid, urea, aluminum chloride, sodium bicarbonate, ammonium sulfate, etc.

[0032] Stabilizers: such as xanthan gum, sodium polyacrylate, magnesium aluminum silicate, etc.

[0033] Those skilled in the art can routinely select and combine the above-mentioned substances according to the target dosage form and application requirements.

[0034] Example 1 Synergistic effect of MeJA and cabbage looper nucleopolyhedrovirus.

[0035] Methyl jasmonate (MeJA) was diluted with anhydrous ethanol and mixed with artificial feed to prepare a feed with a concentration of 5 μg / gMeJA. Early third instar cotton bollworm larvae of uniform size were selected, starved for 12 h, and then fed using the droplet method at a concentration of 3.4 × 10⁻⁶. 6The cabbage looper nucleopolyhedrovirus strain CHN1 (MbMNPV-CHN1) with OBs / mL was then transferred to a feed container containing MeJA feed. The control group consisted of cotton bollworm larvae, each individually transferred to a feed container containing an equal dose of anhydrous ethanol. Each group was replicated in triplicate, with 20 larvae per replicate. The number of larval deaths was recorded daily until all larvae in the treatment groups died or pupated, and the corrected mortality rate was calculated.

[0036] The corrected mortality rates of cotton bollworm after MbMNPV-CHN1 was mixed with MeJA are shown in Table 1. On days 5, 6, and 7 post-treatment, the corrected mortality rates of the MbMNPV-CHN1+MeJA mixed treatment group were significantly higher than those of the MbMNPV-CHN1-only treatment group. The theoretical mortality rate was calculated using the Sakai formula, and the actual mortality rate (P0.05) was calculated as follows: n ) and theoretical mortality rate (P m As shown in Table 2. By comparing P n With P m The effects of the mixture can be determined. The results show that the mixture of 5 μg / g MeJA and MbMNPV-CHN1 has a significant synergistic effect.

[0037] Example 2 Synergistic effect of MeJA with beet armyworm nucleopolyhedrovirus.

[0038] Select uniformly sized late-stage 2nd instar larvae of the beet armyworm, place them in a sterile 24-well plate, and starve them for 24 hours in a constant temperature and light incubator at 28°C. After the larvae naturally molt and enter the 3rd instar, they are ready for use.

[0039] The concentration of beet armyworm nucleopolyhedrovirus (SeMNPV) was determined using a hemocytometer and diluted to a final concentration of 1×10⁻⁶. 6 3×10 5 1×10 5 3×10 4 1×10 4 Five gradients of OBs / mL were used in the experiment. Two treatment groups were set up: the SeMNPV group, containing only virus; and the SeMNPV+MeJA group, in which MeJA was added to the virus suspensions at the above concentrations to achieve a final MeJA concentration of 150 mol / L. A control group consisting only of MeJA without virus was also included.

[0040] Third-instar beet armyworm larvae subjected to starvation were fed with different treatments and concentrations of virus suspension or MeJA solution using the droplet method. Each concentration was replicated three times, with 20 larvae per replicate. After feeding, the larvae were transferred to 24-well plates containing artificial feed and cultured in a 28°C constant temperature and light incubator. Larval mortality was observed and recorded every 24 hours until all larvae died or pupated. Probit analysis was performed on the virus concentration-larval mortality data to compare the median lethal concentration (LC50) between SeMNPV treatment alone and the SeMNPV+MeJA mixture. 50 Calculate the efficiency ratio.

[0041] The toxicity assay results showed that SeMNPV had a low LC50 against 3rd instar larvae of the beet armyworm. 50 1.02×10 5 OBs / mL, while the LC of SeMNPV+MeJA 50 8.09×10 4 OBs / mL. MeJA showed a significant synergistic effect, with a synergistic ratio of 1.26 (see Table 3 for details).

[0042] Example 3 The effects of combining MeJA and SeMNPV on the feeding behavior of beet armyworm.

[0043] Eight chili seedlings of uniform growth were selected and divided into two groups: the first group of four seedlings was sprayed with only SeMNPV solution; the second group of four seedlings was sprayed with MeJA and SeMNPV solutions sequentially. The specific procedures were as follows: 10 mL of distilled water was used as a control, and anhydrous ethanol (final concentration 0.1%) and 100 L of Tween were added to the distilled water. After thorough mixing, the mixture was sprayed onto the four chili seedlings in the first group. Simultaneously, a prepared 300 mol / L MeJA solution was sprayed onto the four chili seedlings in the second group. After 24 h, a prepared 1×10⁻⁶ mol / L MeJA solution was sprayed onto the second group of four seedlings. 6 20 mL of SeMNPV solution with OBs / mL was sprayed evenly onto the eight chili seedlings.

[0044] After spraying with SeMNPV solution, allow the solution to air dry on the chili seedling leaves before transferring all seedlings into a sealed container. Place two seedlings treated differently together, i.e., place eight seedlings alternately so that every two adjacent seedlings represent different treatments. Finally, place a piece of tissue paper between the eight seedlings, and on the tissue paper, place approximately 30 third-instar beet armyworm larvae (see [link to product description]). Figure 1 24 hours after insect inoculation, the leaf area of ​​the tested insects that had been fed on was measured using an intelligent leaf area measurement system.

[0045] The results showed that pepper seedlings treated with SeMNPV alone were significantly more susceptible to damage from the beet armyworm than those treated with SeMNPV+MeJA (see [link to SeMNPV+MeJA treatment]). Figure 2 The feeding area measurement showed that the total feeding area of ​​the four seedlings in the SeMNPV treatment group was 3942.43 mm². 2 The total edible area of ​​the four pepper seedlings in the SeMNPV+MeJA treatment group was 3449.92 mm². 2 The feeding area was 492.51 mm smaller than that of the SeMNPV treatment group. 2 The results indicate that adding MeJA can effectively reduce pest damage and enhance the protective effect of viral insecticides on crops.

[0046] Example 4 The effects of MeJA combined with MbMNPV-CHN1 on the feeding behavior of cotton bollworm.

[0047] This experiment set up 4 treatment groups: Treatment 1, with a concentration of 3.40 × 10⁻⁶. 6 Treatment 2 involved spraying tobacco leaves with a concentration of 3.40 × 10⁻⁶ oz / mL MbMNPV-CHN1 and 10 μg / mL MeJA at a volume ratio of 1:1. 6 MbMNPV-CHN1 at a concentration of OBs / mL was sprayed evenly onto tobacco leaves; Treatment 3, with a concentration of 5.30 × 10⁻⁶. 5 HearNPV (obs / mL) was uniformly sprayed onto tobacco leaves; Treatment 4 involved uniformly spraying tobacco leaves with water (CK). Each treated leaf was inoculated with one early-stage 3rd instar bollworm larva, starved for 12 hours, and isolated under a nylon mesh cover. The larvae's feeding area was measured using a smart leaf area measurement system after 4 days. Each treatment was repeated three times.

[0048] The results showed that the feeding area of ​​cotton bollworms was significantly lower after treatment with HearNPV or MbMNPV-CHN1 alone than that of the control (CK), but there was no significant difference between the two virus treatments alone (see [link to study]). Figure 3 In contrast, the feeding area of ​​the CK and virus-only treatment groups was significantly higher than that of the MbMNPV-CHN1 / MeJA mixture treatment group. These results indicate that the combined use of MeJA and MbMNPV-CHN1 can effectively reduce the feeding damage of cotton bollworms to tobacco.

[0049] Example 5 Preparation of cotton bollworm nucleopolyhedrovirus suspension and its in vitro efficacy test when combined with MeJA.

[0050] Drug solution preparation: Dilute the Hellenella nucleopolyhedrovirus (HearNPV) stock solution, add glycerol, sodium lignosulfonate, and emulsifier, stir well, and add water to prepare a virus suspension. Separately, dissolve MeJA in 0.1% anhydrous ethanol aqueous solution, add Tween-80 to aid dissolution, and prepare a MeJA solution. Mix the virus suspension and MeJA solution in a certain proportion to achieve a final HellNPV concentration of 1×10⁻⁶ in the mixture. 5 The OBs / mL, the final MeJA concentration is 150µmol / L.

[0051] Tobacco seedling treatment: Eighteen tobacco seedlings of uniform growth were selected for each experiment and divided into three groups (see Table 4 for details). The first group of 6 seedlings was sprayed with a concentration of 1×10⁻⁶. 5 The first group of seedlings was treated with a HearNPV suspension containing OBs / mL; the second group of 6 seedlings was sprayed with a MeJA solution at a concentration of 150 µmol / L; the third group of 6 seedlings was sprayed with a mixture of HearNPV and MeJA, with final concentrations of 1×10⁻⁶ and 1×10⁻⁶, respectively. 5 OBs / mL and 150µmol / L.

[0052] Note: The final concentration of HearNPV in each experimental group was 1×10⁻⁶. 5 OBs / mL; the final MeJA concentration in groups two and three was 150 µmol / L. Twelve hours after spraying the pesticide, five leaves were harvested from each of the three groups of tobacco seedlings and cut into squares of uniform shape and size. Leaf discs from different treatments were placed alternately in the same petri dish, and three early-stage fourth-instar bollworm larvae were inoculated into each dish. After 60 hours of continuous feeding, the feeding area was measured. Each treatment was repeated three times, and the average feeding amount was calculated. The results are shown in Table 5. Compared with HearNPV treatment alone, the combined treatment of HearNPV and MeJA significantly reduced the feeding area of ​​tobacco leaves.

[0053] Example 6 In vitro efficacy test of bollworm nucleopolyhedrovirus suspension combined with MeJA against pepper bollworm.

[0054] Preparation of the drug solution: Following the method in Example 5, prepare a solution with a final concentration of 1×10⁻⁶. 5 A mixed suspension of OBs / mL HearNPV and 150µmol / L MeJA was prepared, along with corresponding single-component control solutions.

[0055] Pepper seedling treatment: Eighteen pepper seedlings of uniform growth were selected for each experiment and divided into three groups (see Table 6 for details). The first group was sprayed with a final concentration of 1×10⁻⁶. 5The first group was sprayed with a HearNPV suspension at OBs / mL; the second group was sprayed with a MeJA solution at a final concentration of 150 µmol / L; the third group of 6 seedlings was sprayed with a mixture of HearNPV and MeJA, with final concentrations of 1×10⁻⁶ for each. 5 OBs / mL and 150µmol / L.

[0056] Twelve hours after spraying the pesticide, five leaves were harvested from each of the three groups of pepper seedlings and cut into squares of uniform shape and size. Leaf discs from different treatments were placed alternately in the same petri dish, and three early-stage fourth-instar bollworm larvae were inoculated into each dish. After 60 hours of continuous feeding, the feeding area was measured. Each treatment was repeated three times, and the average feeding amount was calculated. The results, as shown in Table 7, indicate that compared to HearNPV treatment alone, the combined HearNPV and MeJA treatment significantly reduced the feeding area of ​​the pepper leaf discs.

[0057] Example 7 Efficacy of cotton bollworm nucleopolyhedrovirus suspension combined with MeJA against live cotton bollworm in tobacco plants.

[0058] Preparation of the drug solution and treatment of tobacco seedlings: The test drug solution was prepared according to the method in Example 5, and the tobacco seedlings were treated in the same grouping manner.

[0059] After spraying the pesticide, the plant surface was allowed to air dry naturally. Tobacco seedlings from each group were then placed in insect rearing cages, with 20 early-stage third-instar bollworm larvae in each cage. The larvae were allowed to feed freely for 72 hours. The leaf feeding area was then measured afterward. Each treatment was repeated three times, and the average feeding amount was calculated. The results are shown in Table 8. Compared with HearNPV treatment alone, the combined HearNPV and MeJA treatment significantly reduced the leaf feeding area of ​​tobacco plants.

[0060] Example 8 Efficacy of cotton bollworm nucleopolyhedrovirus suspension combined with MeJA against cotton bollworm in live plants.

[0061] Preparation of the drug solution: Refer to Example 5.

[0062] Pepper seedling treatment: Eighteen pepper seedlings of uniform growth were selected for each experiment and divided into three groups. The first group was sprayed with a final concentration of 1×10⁻⁶. 5 The first group was sprayed with a HearNPV suspension at a final concentration of 1000 OBs / mL; the second group was sprayed with a MeJA solution at a final concentration of 250 µmol / L; and the third group was sprayed with a mixture of HearNPV and MeJA at a final concentration of 1×10⁻⁶. 5OBs / mL and 250µmol / L.

[0063] After spraying the pesticide, and allowing the pesticide solution to air dry on the plant surface, each group of pepper seedlings was placed in an insect rearing cage. Each cage contained 20 early-stage third-instar bollworm larvae, who were allowed to feed freely for 72 hours. The pepper seedlings were then removed, and the leaf feeding area was measured. Each treatment was repeated three times, and the average feeding amount was calculated. The results are shown in Table 9. Compared with HearNPV treatment alone, the combined HearNPV and MeJA treatment significantly reduced the leaf feeding area of ​​the pepper plants.

[0064] The examples primarily use methyl jasmonic acid, but this illustrative approach does not constitute any limitation on jasmonic acid. Based on the synergistic novel use of entomopathogenic viruses in combination with jasmonic acid signaling molecules disclosed in this invention, those skilled in the art will understand that jasmonic acid and its methyl ester derivatives can exert equivalent effects based on the same core mechanism in this application scenario. Therefore, those skilled in the art can reasonably expect that jasmonic acid, when used in combination with entomopathogenic viruses, can also achieve the objectives of this invention.

[0065] It should be noted that the above embodiments are only used to illustrate the present invention, but the present invention is not limited to the above embodiments. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention shall fall within the protection scope of the present invention.

Claims

1. An insecticidal composition, characterized in that: It contains insect pathogen viruses and methyl jasmonate or jasmonic acid as active ingredients.

2. The insecticidal composition according to claim 1, characterized in that: The insect pathogenic virus is a baculoviridae virus.

3. The insecticidal composition according to claim 2, characterized in that: The baculoviridae virus is a nucleopolyhedrovirus.

4. The insecticidal composition according to claim 3, characterized in that: The nucleopolyhedrovirus was selected from cotton bollworm nucleopolyhedrovirus, beet armyworm nucleopolyhedrovirus, or cabbage armyworm nucleopolyhedrovirus.

5. The insecticidal composition according to claim 1, characterized in that: The mass ratio of the insect pathogen virus to methyl jasmonate or jasmonic acid is 1:(0.5-1000).

6. The insecticidal composition according to claim 5, characterized in that: The mass ratio is 1:(5-500).

7. The insecticidal composition according to claim 1, characterized in that: The composition also includes pesticide-acceptable carriers and adjuvants, and is formulated as a suspension concentrate, emulsion, water-dispersible granule, or wettable powder.

8. A method for controlling lepidopteran pests, characterized in that: Methyl jasmonate or jasmonic acid is applied in combination with insect baculovirus to the target pest or its living environment. The combined application means that the insect pathogen virus is applied at the same time as methyl jasmonate or jasmonic acid, or the insect pathogen virus is applied first, and then methyl jasmonate or jasmonic acid is applied after an interval of 1 to 7 days.

9. The method according to claim 8, characterized in that: The lepidopteran pests mentioned are cotton bollworm, beet armyworm, or fall armyworm.

10. Use of the insecticidal composition according to any one of claims 1 to 7 in the control of lepidopteran pests.

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