Agrochemical-free and harmless indoor plant maintenance spray for pets and preparation method of agricultural-chemical-free and harmless indoor plant maintenance spray

Abstract

The invention relates to the technical field of indoor plant maintenance, and provides an agricultural-chemical-free pet-harmless indoor plant maintenance spray and a preparation method thereof.The spray is prepared from composite plant fermentation broth, plant raw materials are selected from dandelion, chrysanthemum, mint, honeysuckle, purple perilla and other varieties harmless to pets, and the plant raw materials are selected from natural plant raw materials. The composite strains comprise disease-resistant and insect-proof strains, nutrition conversion strains and growth promotion strains, and through the synergistic effect of a reasonable mass ratio, triple effects of expelling insects, killing pathogenic bacteria and applying fertilizer are achieved. The preparation method comprises the steps of plant raw material treatment, strain activation, fermentation treatment and post-treatment, and the spraying agent meeting the requirements is prepared by fermenting a single plant or respectively fermenting mixed plants and then mixing according to a proportion under the conditions of proper temperature and pH. The problems that a traditional product is single in function, contains agrochemical components and is harmful to pets are solved, and the pet food is suitable for the indoor environment with pets and children and has high safety and practicability.

Description

Technical Field

[0001] This invention relates to the field of indoor plant care technology, specifically to an agrochemical-free indoor plant care spray that is harmless to pets and its preparation method. Background Technology

[0002] The current indoor plant care industry faces numerous technical challenges: traditional methods require the separate use of insecticides, fungicides, and fertilizers, which are cumbersome and prone to causing chemical residues, harming the indoor environment and the health of pets and young children. Many products contain chemical pesticides or use ingredients harmful to pets, such as garlic, onions, and toxic herbs, posing safety hazards.

[0003] Meanwhile, existing fermentation products suffer from unreasonable strain combinations and limited functionality, making it difficult to achieve synergistic effects of insect repellency, sterilization, and fertilization. Furthermore, they exhibit poor adaptability to specific indoor environments; for example, insufficient light leads to low photosynthetic efficiency in plants, pet excrement easily causes substrate decay and odor, and the activity of microorganisms is unstable in environments with large temperature fluctuations. In addition, mixed plant fermentation often suffers from imbalanced efficacy due to raw material compatibility issues, and crude preparation processes result in poor product stability.

[0004] Therefore, an agricultural-free indoor plant care spray and its preparation method are proposed, and a plant care solution that is agricultural-free, harmless to pets, multifunctional, and suitable for indoor environments is developed. Summary of the Invention

[0005] Technical problems to be solved The current indoor plant care industry faces numerous technical challenges: traditional methods require the separate use of insect repellents, fungicides, and fertilizers, which are cumbersome and prone to causing chemical residues, harming the indoor environment and the health of pets and young children. Most products contain chemical pesticides or use ingredients harmful to pets, such as garlic, onions, and toxic herbs, posing safety hazards. Furthermore, existing fermented products often have unbalanced microbial strains and limited functionality, failing to synergistically achieve insect repellency, sterilization, and fertilization. They also exhibit poor adaptability to specific indoor environments; for example, insufficient light leads to low photosynthetic efficiency, pet excrement can cause substrate decay and odor, and microbial activity is unstable in environments with large temperature fluctuations. In addition, mixed plant fermentation often suffers from incompatibility issues with raw materials, resulting in unbalanced efficacy, and crude manufacturing processes lead to poor product stability.

[0006] Technical solution To achieve the above-mentioned objectives, the present invention provides the following technical solution: an indoor plant care spray that is pesticide-free and harmless to pets, wherein the spray is made from a compound plant fermentation liquid, the compound plant fermentation liquid comprising plant raw materials and compound microbial fermentation products, the specific composition of which is as follows: The plant material is selected from at least one of dandelion, chrysanthemum, mint, honeysuckle, and perilla; The compound microbial strain includes disease-resistant and insect-repellent strains, nutrient-converting strains, and growth-promoting strains; wherein, the disease-resistant and insect-repellent strains are selected from at least two of Bacillus licheniformis, Trichoderma harzianum, Paecilomyces lilacinus, Bacillus thuringiensis, and actinomycetes; the nutrient-converting strains are selected from at least two of Bacillus subtilis, Bacillus mucilaginosus, Bacillus megaterium, azotobacter brownii, and azotobacter chroococcus; and the growth-promoting strains are selected from at least one of yeast, photosynthetic bacteria, Bacillus laterosporus, and Bacillus amyloliquefaciens. The mass ratio of the plant raw materials to the compound microbial strain is 10-20:1; The mass percentage of each strain in the compound microbial strain is as follows: 30%–40% for disease-resistant and insect-resistant strains, 40%–50% for nutrient conversion strains, and 10%–30% for growth-promoting strains.

[0007] Preferably, the plant material is a combination of two or more of the following: dandelion, chrysanthemum, mint, honeysuckle, and perilla.

[0008] Preferably, the disease-resistant and insect-resistant bacterial strain is one of the following combinations: Bacillus licheniformis and Trichoderma harzianum, Paecilomyces lilacinus and Bacillus thuringiensis, or Actinomycetes and Bacillus licheniformis.

[0009] Preferably, the nutrient conversion bacteria are one of the following combinations: Bacillus subtilis and Bacillus mucilaginosus, Bacillus megaterium and Azotobacter chrysozoans, or Azotobacter spp. and Bacillus subtilis.

[0010] Preferably, the growth-promoting bacteria are one of the following: yeast, a combination of photosynthetic bacteria and Bacillus laterosporus, or Bacillus amyloliquefaciens.

[0011] A method for preparing an agrochemical-free and pet-friendly indoor plant care spray, the method being applied to the aforementioned agrochemical-free and pet-friendly indoor plant care spray, the method comprising the following steps: S1. Plant raw material processing: Select at least one of dandelion, chrysanthemum, mint, honeysuckle and perilla as plant raw materials, wash them with deionized water and then crush them. The particle size of the crushed particles is controlled at 0.5-1mm. Then add deionized water to make a plant slurry with a mass concentration of 10%-20%. S2. Strain Activation: Each strain from the disease-resistant, insect-repellent, nutrient-converting, and growth-promoting strains is inoculated into its corresponding sterile culture medium and incubated at 25-30℃ for 24-48 hours to activate the strain, obtaining a single-strain bacterial suspension. The viable count of the single-strain bacterial suspension should reach 10-1. 9 -10 10 CFU / mL; S3. Fermentation treatment: There are two methods of fermentation treatment: single plant fermentation and mixed plant fermentation; During the single-plant fermentation, the compound microbial strain is inoculated into the plant slurry at a mass ratio of plant raw material to compound microbial strain of 10-20:1. Fermentation is carried out for 5-7 days at a temperature of 28-32℃ and a pH value of 6.0-7.0. The mixture is stirred 1-2 times a day during the fermentation process to obtain the fermentation liquid. During the mixed plant fermentation, multiple plants are made into slurries, and then inoculated with compound strains or single strains for fermentation. The fermentation broths of each single plant are then mixed in a volume ratio of 1-3:1-3:1-3 to obtain a mixed plant fermentation broth. S4. Post-processing: Filter the fermentation broth after fermentation, and dilute the filtrate with deionized water until the viable cell count is 10⁻⁶. 8 -10 9 The prepared spray (CFU / mL) is placed in a spray bottle and sealed for storage at a temperature below 25°C and a humidity below 60%.

[0012] Preferably, in the plant material processing, the slurry made from dandelion has a mass concentration of 12%, and the slurry made from mint has a mass concentration of 18%.

[0013] Preferably, in the strain activation step, Bacillus licheniformis is cultured on beef extract peptone medium containing 3 g / L beef extract, 10 g / L peptone, and 5 g / L NaCl; Trichoderma harzianum is cultured on PDA medium containing 200 g / L potato starch, 20 g / L glucose, and 15 g / L agar.

[0014] Preferably, in the fermentation process, when using single-plant fermentation, the fermentation tank capacity is 1.5-2 times the volume of the plant slurry; when using mixed-plant fermentation, the plants are fermented separately and then mixed: the various plants are made into slurries and fermented separately for 5-7 days, and then the fermentation liquids of each single plant are mixed in a volume ratio of 1-3:1-3:1-3.

[0015] Preferably, in the post-processing step, the filtration is carried out by either gauze filtration or centrifugal filtration; when filtering with gauze, 80-100 mesh gauze is selected, and the filtration is repeated 2 to 3 times; when centrifuging, the centrifugation speed is 3000-4000 r / min, the centrifugation time is 10 to 15 min, and the supernatant is taken.

[0016] Beneficial effects Compared with existing technologies, this invention provides an agrochemical-free and pet-friendly indoor plant care spray and its preparation method, which has the following beneficial effects: 1. This solution achieves multifunctional synergistic effects through the scientific combination of compound microbial strains, breaking through the limitations of existing single-function maintenance products. Disease-resistant, insect-repellent, nutrient-converting, and growth-promoting microbial strains are combined in specific proportions and work synergistically with plant extracts. This not only inhibits pathogens and pests but also activates soil nutrients, enhances the plant's ability to absorb nutrients, and promotes root growth and photosynthesis, forming a complete maintenance loop of "insect control—energy supply—growth promotion," enabling plant maintenance to achieve comprehensive and highly efficient results.

[0017] 2. This solution fundamentally avoids the toxic risks that existing plant care products may pose to pets by selecting plant-based ingredients that are harmless to pets. The plants used are not only safe, but their extracts also work synergistically with the compound microbial strains, solving the problem of balancing effective ingredients and pet safety in traditional care products. This allows pet-owning families to care for indoor plants without worrying about their pets' health.

[0018] 3. This solution utilizes plant-based materials and natural microorganisms for fermentation, without adding any chemical pesticides or synthetic ingredients, thus avoiding pesticide residue pollution and soil compaction problems associated with traditional chemical plant care products. The plant-based materials are biodegradable, and the compound microbial strains can optimize the soil's micro-ecological environment, maintaining indoor ecological balance. This aligns with the trend of green and sustainable development, providing an environmentally friendly new option for indoor plant care.

[0019] 4. This solution effectively solves the problem of unstable effects caused by component conflicts in existing mixed plant fermentation technologies by using single-plant fermentation or mixed plant fermentation followed by compounding, tailored to the characteristics of different plants. This flexible preparation method allows for formula adjustments based on the needs of different plants, ensuring stable fermentation efficiency and effects, improving the product's versatility for various indoor plants, and enhancing its operability in practical applications. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the spray components of the present invention; Figure 2 This is a schematic diagram of the preparation process of the present invention. Detailed Implementation

[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] Please see Figures 1-2This invention proposes an agrochemical-free, pet-friendly indoor plant care spray and its preparation method, comprising the following: An agricultural-free and pet-friendly indoor plant care spray is made from a compound plant fermentation liquid. The compound plant fermentation liquid includes plant raw materials and compound microbial fermentation products. The specific selection and formulation of each component are as follows: 1. Selection of plant raw materials: The ingredients are selected from pet-friendly plant sources, specifically at least one of dandelion, chrysanthemum, mint, honeysuckle, and perilla. These plants are not only safe for pets themselves, but their extracts also have certain insect-repellent and antibacterial effects, which can work synergistically with the compound microbial strains.

[0023] 2. Selection of compound microbial strains: Compound microbial strains include disease-resistant and insect-resistant strains, nutrient-converting strains, and growth-promoting strains, as detailed below: 3. Disease- and insect-resistant fungal strains: selected from at least two of the following: Bacillus licheniformis, Trichoderma harzianum, Paecilomyces lilacinus, Bacillus thuringiensis, and actinomycetes. Among them, Bacillus licheniformis can inhibit the growth of pathogens and alleviate soil-borne diseases; Trichoderma harzianum can form a protective barrier and decompose the cell walls of pathogenic fungi; Paecilomyces lilacinus can control root-knot nematodes; Bacillus thuringiensis has a specific toxic effect on lepidopteran pests; and actinomycetes can secrete natural fungicides to inhibit a variety of pathogens.

[0024] 4. Nutrient conversion microorganisms: Selected from at least two of Bacillus subtilis, Bacillus mucilaginosus, Bacillus megaterium, Azotobacter browniformis, and Azotobacter chroococcus. Bacillus subtilis can activate soil nutrients, solubilize phosphorus and potassium, and fix nitrogen; Bacillus mucilaginosus can release soluble phosphorus, potassium, and various trace elements; Bacillus megaterium can degrade organic phosphorus and improve phosphorus utilization; Azotobacter browniformis and Azotobacter chroococcus can fix nitrogen from the air and increase soil nitrogen content.

[0025] 5. Growth-promoting microorganisms: selected from at least one of yeast, photosynthetic bacteria, Bacillus laterosporus, and Bacillus amyloliquefaciens. Yeast can ferment organic matter, loosen the soil, and enhance water retention; photosynthetic bacteria can promote photosynthesis and activate plant cells when light is insufficient; Bacillus laterosporus can promote root growth and enhance nutrient absorption capacity; Bacillus amyloliquefaciens can secrete plant growth hormones, promoting cell division and root expansion.

[0026] 6. Proportioning relationship: In the compound plant fermentation broth, the mass ratio of plant raw materials to compound microbial strains is 10-20:1.

[0027] The mass proportions of each microbial species in the compound are as follows: 30%–40% for disease-resistant and insect-repellent species, 40%–50% for nutrient conversion species, and 10%–30% for growth-promoting species. This ratio ensures synergistic effects among the species and fully leverages their triple efficacy.

[0028] A method for preparing an agricultural-free, pet-friendly indoor plant care spray includes the following steps: 1. Plant material processing: When processing plant materials, at least one of the following is selected: dandelion, chrysanthemum, mint, honeysuckle, and perilla. The plant materials are thoroughly cleaned with deionized water to remove surface impurities and dirt. Deionized water, rather than tap water, is used to avoid the chlorine in tap water damaging the active components of the plants, such as menthol and chlorogenic acid. The cleaned plant materials are then pulverized in a grinder. The particle size is controlled between 0.5 and 1 mm. The lower limit of 0.5 mm ensures that plant cells rupture, releasing internal nutrients, such as flavonoids in chrysanthemum, which are beneficial for microbial utilization. The upper limit of 1 mm prevents excessively fine particles from causing sedimentation and affecting fermentation uniformity. The pulverized plant materials are then added to deionized water and stirred evenly to prepare a plant slurry with a mass concentration of 10%–20%. The mass concentration needs to be adjusted according to the characteristics of the plant materials. For example, dandelion has a high fiber content, so a slurry with a mass concentration of 12% can be prepared; mint has a softer texture, so a slurry with a mass concentration of 18% can be prepared.

[0029] 2. Strain activation: During strain activation, each strain from the disease-resistant, insect-resistant, nutrient-converting, and growth-promoting strains is inoculated into its corresponding sterile culture medium. The culture medium formulations differ for different strains. For example, *Bacillus licheniformis* can be cultured on beef extract peptone medium containing 3 g / L beef extract, 10 g / L peptone, and 5 g / L NaCl to provide nitrogen and carbon sources; *Trichoderma harzianum* can be cultured on PDA medium containing 200 g / L potato starch, 20 g / L glucose, and 15 g / L agar to simulate its natural growth environment and promote mycelial growth. After inoculation, the culture medium is placed in a constant temperature incubator at 25-30℃ for 24-48 hours for activation. Bacteria metabolize quickly and can be activated in 24 hours, while fungi grow slowly and require 48 hours to ensure full activation and recovery of activity for each strain, yielding single-strain bacterial suspensions. The viable count of a single-strain bacterial suspension should reach 10-1. 9 -10 10 CFU / mL.

[0030] 3. Fermentation treatment: There are two fermentation methods. If single-plant fermentation is used, the single-plant slurry is placed in a fermentation tank. The tank capacity is selected based on the amount of slurry, generally 1.5-2 times the slurry volume, leaving space for the diffusion of gases such as carbon dioxide produced by the microbial metabolism to avoid excessive pressure. The compound microbial culture is inoculated into the plant slurry at a mass ratio of plant raw material to compound microbial culture of 10-20:1. The mass percentage of each microbial species in the compound microbial culture is as follows: 30%-40% for disease and insect resistance, 40%-50% for nutrient conversion, and 10%-30% for growth promotion. After inoculation, the temperature inside the fermentation tank is adjusted to 28-32℃, which is the logarithmic growth phase temperature for most microbial species, such as Bacillus mucilaginosa, which is optimal at 30℃. The pH value is controlled at 6.0-7.0; a slightly acidic to neutral environment is suitable for the activity of most microbial species, such as actinomycetes, which are optimally at pH 6.5. During the fermentation process, the mixture is stirred 1 to 2 times a day for 10 to 15 minutes each time to ensure that the inoculum and plant slurry are in full contact and to improve the fermentation efficiency. The fermentation time is 5 to 7 days, and a single plant fermentation liquid is obtained after the fermentation is completed.

[0031] If mixed plant fermentation is used, and there are technical difficulties in fermenting multiple plants together, a method of separate fermentation followed by mixing is adopted. Multiple plants are processed into slurries using the same method as single-plant raw materials. Each slurry is then inoculated separately with a compound microbial strain or a single microbial strain. If a single microbial strain is used, it must be ensured that the strain covers three categories: disease resistance and insect control, nutrient conversion, and growth promotion. These slurries are fermented separately for 5–7 days at 28–32℃ and pH 6.0–7.0, with stirring 1–2 times daily to obtain a single-plant fermentation liquid. Then, the single-plant fermentation liquids are mixed in a volume ratio of 1–3:1–3:1–3, for example, dandelion fermentation liquid: chrysanthemum fermentation liquid: mint fermentation liquid = 2:2:1. After thorough mixing, a mixed plant fermentation liquid is obtained. This method allows for flexible adjustment to suit the characteristics of the plants; for example, a ratio of "dandelion: chrysanthemum = 2:1" can enhance antibacterial and nitrogen-fixing effects, while a ratio of "mint: honeysuckle = 3:2" can improve insect repellent and growth-promoting effects.

[0032] 4. Post-processing: In post-processing, the fermented broth is filtered, either using gauze filtration or centrifugal filtration. For gauze filtration, use 80-100 mesh gauze and repeat filtration 2-3 times to remove solid impurities. 80 mesh is suitable for broths with high fiber content, while 100 mesh is suitable for broths containing fine particles, such as chrysanthemum slurry. For centrifugal filtration, the centrifugation speed is 3000-4000 rpm for 10-15 minutes. The supernatant is then collected, and impurities are separated by centrifugation, which is more efficient and suitable for large-scale production. 3000 rpm removes large particles, while 4000 rpm is suitable for fine filtration. After filtration, the filtrate is diluted with deionized water until the viable cell count is 10⁻⁶. 8 -10 9CFU / mL, lower limit 10 8 CFU / mL is to ensure that each 1mL spray provides approximately 10 live bacteria. 8 Each one can exert its effect, with a maximum of 10. 9 The CFU / mL concentration is designed to prevent excessive live bacteria from causing "microbial competition" on plant leaves and generating metabolic waste. The prepared spray is then placed in a spray bottle, sealed, and stored in a cool, dry place at a temperature <25℃ and humidity <60%. This slows down the metabolic rate of the bacteria and extends the shelf life to 6-12 months.

[0033] Example Example 1: Single Plant (Peppermint) Fermented Spray Plant ingredients: 1000g of mint.

[0034] Compound microbial strains: Total weight 100g. Disease and insect resistance 30g (Bacillus licheniformis 15g + Trichoderma harzianum 15g); nutrient conversion 50g (Bacillus subtilis 25g + Bacillus mucilaginosus 25g); growth promotion 20g (yeast 20g).

[0035] Preparation steps: Wash and crush the peppermint to 0.5-1 mm, then add deionized water to make an 18% slurry. Activate the bacterial strain according to the corresponding culture medium until the viable count reaches 10⁻⁶. 9 CFU / mL. The peppermint slurry was transferred to a 2L fermenter, inoculated with a compound microbial culture, and fermented at 30℃ and pH 6.5, stirring twice daily (10 min each time) for 6 days. The mixture was then filtered three times through 100-mesh gauze and diluted to a viable count of 5 × 10⁻⁶. 8 CFU / mL, bottled and sealed for storage.

[0036] Example 2: Mixed plants (dandelion + honeysuckle) were fermented separately and then mixed into a spray. Plant ingredients: 800g dandelion + 600g honeysuckle.

[0037] Compound microbial strains: Total weight 100g. Disease and insect resistance 40g (Paecilomyces lilacinus 20g + Bacillus thuringiensis 20g); nutrient conversion 40g (Bacillus megaterium 20g + Azotobacter chrysotrichum 20g); growth promotion 20g (photosynthetic bacteria 20g).

[0038] Preparation steps: Dandelion and honeysuckle are washed and pulverized separately. Dandelion is made into a 12% slurry, and honeysuckle is made into a 15% slurry. After activation, the viable bacteria count reaches 10. 9 -10 10 CFU / mL. Inoculate each of the two slurries with 50g of the compound bacterial culture and ferment at 30℃ and pH 6.5 for 7 days, stirring once daily (15min). Mix dandelion fermentation broth and honeysuckle fermentation broth at a ratio of 2:1, centrifuge at 3000r / min for 15min, and dilute to a viable count of 8×10⁻⁶.8 CFU / mL, bottled.

[0039] Example 3: Fermented spray containing three plants (chrysanthemum, mint, and perilla) Plant ingredients: 500g chrysanthemum + 500g mint + 500g perilla.

[0040] Compound microbial strains: Total weight 100g. Disease and insect resistance 35g (actinomycetes 15g + Bacillus thuringiensis 20g); nutrient conversion 45g (brown nitrogen-fixing bacteria 20g + Bacillus subtilis 25g); growth promotion 20g (Bacillus laterosporus 10g + Bacillus amyloliquefaciens 10g).

[0041] Preparation steps: The three plants were mixed and pulverized to prepare a 16% mixed slurry. After activation, the inoculum was inoculated into the slurry and fermented at 28℃, pH 7.0, stirring twice daily (12 min each time) for 5 days. The mixture was then centrifuged at 4000 rpm for 10 min, filtered twice through 80-mesh gauze, and diluted to a viable count of 10-1. 9 CFU / mL, bottled.

[0042] Comparative Example Comparative Example 1: Plant-based ingredients contain components harmful to pets Plant ingredients: 500g lily bulbs + 500g mint (total 1000g; lily bulbs are toxic to cats and do not meet the requirement of being harmless to pets).

[0043] Compound microbial strain: Total weight 100g (plant raw materials: compound microbial strain = 10:1).

[0044] Disease- and insect-resistant compound 30g (30% of total weight): Bacillus licheniformis 15g + Trichoderma harzianum 15g; Nutritional conversion supplement 50g (50% of total weight): Bacillus subtilis 25g + Bacillus mucilaginosus 25g; Growth promoter 20g (accounting for 20%): Yeast 20g.

[0045] Preparation steps: Lily bulbs and mint were washed and crushed to 0.5-1 mm. Mint was made into an 18% slurry and lily bulbs into a 15% slurry. Strain activation: Cultured on the corresponding culture medium until the viable count reaches 10. 9 CFU / mL; Mixed fermentation: The two slurries are mixed and put into a 2L fermentation tank (slurry volume 1L), inoculated with compound bacteria, and the temperature is adjusted to 30℃ and pH 6.5. Stir twice a day (10min each time) for 6 days. Post-treatment: Filter three times through 100-mesh gauze, then dilute with deionized water to a viable bacteria count of 5 × 10⁻⁶. 8 CFU / mL, bottled and sealed (store below 25°C).

[0046] Comparative Example 2: Deviation of Compound Microbial Culture Ratio from the Range Plant ingredients: 800g dandelion + 600g honeysuckle (total 1400g).

[0047] Compound microbial strain: Total weight 100g (plant raw materials: compound microbial strain = 14:1).

[0048] Disease- and insect-resistant agent 10g (accounting for 10%, lower than the document's 30% to 40% range): Paecilomyces lilacinus 5g + Bacillus thuringiensis 5g; 80g of nutrient conversion products (80% of the total content, higher than the 40% to 50% range of the document): Bacillus megaterium 40g + Azotobacter chrysophagus 40g; Growth promoter 10g (10% of total weight): Photosynthetic bacteria 10g.

[0049] Preparation steps: Dandelion was made into a 12% slurry, and honeysuckle was made into a 15% slurry; Strain activation: Cultured on the corresponding culture medium until the viable count reaches 10. 9 -10 10 CFU / mL; Separate fermentation: 50g of compound bacteria (same ratio as above) were added to each of the two slurries. The fermentation tank temperature was 30℃ and pH was 6.5. The mixture was stirred once a day (15min) for 7 days to obtain two single fermentation liquids. Mixing and post-treatment: Mix dandelion fermentation broth and honeysuckle fermentation broth at a ratio of 2:1 (volume ratio), centrifuge at 3000 r / min for 15 min, collect the supernatant, and dilute to a viable count of 8 × 10⁻⁶. 8 CFU / mL, bottled.

[0050] Comparative Example 3: Fermentation temperature deviates from the optimal range Plant ingredients: 500g chrysanthemum + 500g mint + 500g perilla (total 1500g).

[0051] Compound microbial strain: Total weight 100g (plant raw materials: compound microbial strain = 15:1).

[0052] Disease-resistant and insect-resistant 35g (35% of total weight): Actinomycetes 15g + Bacillus thuringiensis 20g; Nutritional conversion agent 45g (45% of total weight): 20g of azotobacter brownii + 25g of Bacillus subtilis; Growth promoter 20g (accounting for 20%): Bacillus laterosporus 10g + Bacillus amyloliquefaciens 10g.

[0053] Preparation steps: The three plants were mixed and pulverized to 0.5-1 mm, and then deionized water was added to make a 16% mixed slurry. After the inoculum is activated, it is mixed and inoculated into the slurry. The fermentation temperature is controlled at 20℃ (lower than the document range of 28-32℃), pH 7.0, and stirred twice a day (12 min). Fermentation lasts for 5 days. Centrifuge at 4000 rpm for 10 min, collect the supernatant, filter twice through 80-mesh gauze, and dilute to a viable count of 10. 9 CFU / mL, bottled.

[0054] Comparative Example 4: Deviation in the ratio of plant raw materials to compound microbial strains Plant ingredients: 1000g of mint.

[0055] Compound microbial strain: Total weight 200g (plant raw material: compound microbial strain = 5:1, lower than the document range of 10-20:1, excessive microbial strain).

[0056] Disease- and insect-resistant 60g (30% of total weight): Bacillus licheniformis 30g + Trichoderma harzianum 30g; Nutritional conversion 100g (50% of total weight): Bacillus subtilis 50g + Bacillus mucilaginosus 50g; Growth promoter 40g (accounting for 20%): Yeast 40g.

[0057] Preparation steps: Peppermint was washed and crushed to 0.5-1 mm, and then mixed with deionized water to make an 18% slurry. Strain activation: Cultured on the corresponding culture medium until the viable count reaches 10. 9 CFU / mL; Fermentation: Put the peppermint slurry into a 3L fermentation tank (slurry volume 1L), add 200g of compound bacteria, adjust the temperature to 30℃ and pH to 6.5, stir twice a day (10min each time), and ferment for 6 days; Post-treatment: Filter three times through 100-mesh gauze, then dilute with deionized water to a viable bacteria count of 5 × 10⁻⁶. 8 CFU / mL, bottled and sealed (store below 25°C).

[0058] Expected results: Excessive bacterial strains lead to intense competition among the bacterial community and the accumulation of metabolic waste (such as organic acids), which in turn inhibits plant root growth and reduces the stability of the spray, shortening the shelf life to less than 3 months.

[0059] The comparison results of various indicators between the examples and the comparative examples are shown in Table 1 below: Table 1. Comparison of various indicators between the examples and the comparative examples

[0060] Therefore, it can be seen that although Comparative Example 1 retains some insect-repellent and nutrient conversion effects, the percentage of the compound component poses a toxic risk to pets and does not meet the safety standards for use. Comparative Example 2, due to an insufficient proportion of disease- and insect-resistant strains, shows a significant decrease in its ability to inhibit root-knot nematodes, lepidopteran pests, and pathogens, making plants more susceptible to pests and diseases. Comparative Example 3 suffers from reduced metabolic activity of the strains due to low temperatures (e.g., Bacillus mucilaginosus and Actinomycetes have an optimal temperature of around 30℃), incomplete fermentation, and insufficient viable cell count, resulting in a significant weakening of its plant growth-promoting and disease- and insect-resistant effects. Comparative Example 4, with an excessive amount of strains, leads to intense competition among the microbial community and the accumulation of metabolic waste (such as organic acids), which in turn inhibits plant root growth and reduces the stability of the spray, shortening its shelf life to less than 3 months. Examples 1-3 strictly followed technical parameters such as "selection of plant raw materials that are harmless to pets", "compound strain ratio (30%~40%:40%~50%:10%~30%)", "fermentation temperature 28-32℃", and "plant to strain ratio 10-20:1", achieving a balance of "safety (harmless to pets), efficacy (disease and insect prevention + nutrient conversion + growth promotion)" and "stability (long shelf life)," thus verifying the scientific validity of this scheme.

[0061] Finally, it should be noted that the above embodiments are merely examples for clearly illustrating the present invention and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. An agricultural-free and pet-friendly indoor plant care spray, characterized in that: The spray is made from a compound plant fermentation liquid, which includes plant raw materials and compound microbial fermentation products, with the following specific composition: The plant material is selected from at least one of dandelion, chrysanthemum, mint, honeysuckle, and perilla; The compound microbial strain includes disease-resistant and insect-repellent strains, nutrient-converting strains, and growth-promoting strains; wherein, the disease-resistant and insect-repellent strains are selected from at least two of Bacillus licheniformis, Trichoderma harzianum, Paecilomyces lilacinus, Bacillus thuringiensis, and actinomycetes; the nutrient-converting strains are selected from at least two of Bacillus subtilis, Bacillus mucilaginosus, Bacillus megaterium, azotobacter brownii, and azotobacter chroococcus; and the growth-promoting strains are selected from at least one of yeast, photosynthetic bacteria, Bacillus laterosporus, and Bacillus amyloliquefaciens. The mass ratio of the plant raw materials to the compound microbial strain is 10-20:1; The mass percentage of each strain in the compound microbial strain is as follows: 30%–40% for disease-resistant and insect-resistant strains, 40%–50% for nutrient conversion strains, and 10%–30% for growth-promoting strains.

2. The indoor plant care spray that is pesticide-free and harmless to pets according to claim 1, characterized in that: The plant materials are two or more of the following: dandelion, chrysanthemum, mint, honeysuckle, and perilla.

3. The indoor plant care spray that is pesticide-free and harmless to pets according to claim 1, characterized in that: The disease-resistant and insect-resistant bacterial species are one of the following combinations: Bacillus licheniformis and Trichoderma harzianum, Paecilomyces lilacinus and Bacillus thuringiensis, or Actinomycetes and Bacillus licheniformis.

4. The indoor plant care spray that is pesticide-free and harmless to pets according to claim 1, characterized in that: The nutrient conversion bacteria are one of the following combinations: Bacillus subtilis and Bacillus mucilaginosus, Bacillus megaterium and Azotobacter chrysozoans, or Azotobacter aubergina and Bacillus subtilis.

5. The indoor plant care spray that is pesticide-free and harmless to pets according to claim 1, characterized in that: The growth-promoting bacteria are one of the following: yeast, a combination of photosynthetic bacteria and Bacillus laterosporus, or Bacillus amyloliquefaciens.

6. A method for preparing an agricultural-free, pet-friendly indoor plant care spray, characterized in that: The method is applied to the non-agrochemical, pet-friendly indoor plant care spray according to any one of claims 1 to 5, and the method includes the following steps: S1. Plant raw material processing: Select at least one of dandelion, chrysanthemum, mint, honeysuckle and perilla as plant raw materials, wash them with deionized water and then crush them. The particle size of the crushed particles is controlled at 0.5-1mm. Then add deionized water to make a plant slurry with a mass concentration of 10%-20%. S2. Strain Activation: Each strain from the disease-resistant, insect-repellent, nutrient-converting, and growth-promoting strains is inoculated into its corresponding sterile culture medium and incubated at 25-30℃ for 24-48 hours to activate the strain, obtaining a single-strain bacterial suspension. The viable count of the single-strain bacterial suspension should reach 10-1. 9 -10 10 CFU / mL; S3. Fermentation treatment: There are two methods of fermentation treatment: single plant fermentation and mixed plant fermentation; During the single-plant fermentation, the compound microbial strain is inoculated into the plant slurry at a mass ratio of plant raw material to compound microbial strain of 10-20:

1. Fermentation is carried out for 5-7 days at a temperature of 28-32℃ and a pH value of 6.0-7.

0. The mixture is stirred 1-2 times a day during the fermentation process to obtain the fermentation liquid. During the mixed plant fermentation, multiple plants are made into slurries, and then inoculated with compound strains or single strains for fermentation. The fermentation broths of each single plant are then mixed in a volume ratio of 1-3:1-3:1-3 to obtain a mixed plant fermentation broth. S4. Post-processing: Filter the fermentation broth after fermentation, and dilute the filtrate with deionized water until the viable cell count is 10⁻⁶. 8 -10 9 The prepared spray (CFU / mL) is placed in a spray bottle and sealed for storage at a temperature below 25°C and a humidity below 60%.

7. The method for preparing an agrochemical-free, pet-friendly indoor plant care spray according to claim 6, characterized in that: In the plant material processing, the slurry made from dandelion has a mass concentration of 12%, and the slurry made from mint has a mass concentration of 18%.

8. The method for preparing an agrochemical-free, pet-friendly indoor plant care spray according to claim 6, characterized in that: In the strain activation step, Bacillus licheniformis was cultured on beef extract peptone medium containing 3 g / L beef extract, 10 g / L peptone, and 5 g / L NaCl; Trichoderma harzianum was cultured on PDA medium containing 200 g / L potato starch, 20 g / L glucose, and 15 g / L agar.

9. The method for preparing an agricultural-free, pet-friendly indoor plant care spray according to claim 6, characterized in that: In the fermentation process, when using single-plant fermentation, the fermentation tank capacity is 1.5-2 times the volume of the plant slurry; when using mixed-plant fermentation, the plants are fermented separately and then mixed: the various plants are made into slurries and fermented separately for 5-7 days, and then the fermentation liquids of each single plant are mixed in a volume ratio of 1-3:1-3:1-3.

10. The method for preparing an agrochemical-free, pet-friendly indoor plant care spray according to claim 6, characterized in that: In the post-processing steps, filtration is carried out using either gauze filtration or centrifugal filtration. When using gauze filtration, 80-100 mesh gauze is selected, and filtration is repeated 2-3 times. When using centrifugal filtration, the centrifugation speed is 3000-4000 r / min, and the centrifugation time is 10-15 min to collect the supernatant.

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