Microelement composite additive for improving reproductive performance of pets

Abstract

The invention belongs to the technical field of pet nutrition, and discloses a trace element composite additive for improving the reproductive performance of pets, which comprises the following raw materials: zinc sulfate, sodium selenite, copper sulfate, manganese sulfate, vitamin E acetate, L-lysine hydrochloride, taurine, methionine, selenium yeast and zeolite powder. The zeolite powder is subjected to a specific acid treatment activation process, so that the adsorbability and the stability are improved. The invention also discloses a preparation method of the additive. A three-step mixing process is adopted to ensure the uniformity of the product. The additive is suitable for being added into feed in the breeding period of canine or feline pets, the adding amount of the additive is 0.1-0.5% of the total weight of the feed, and the feeding time is 1 month before breeding and 1 month after breeding. Through scientific proportioning of organic trace elements and inorganic trace elements, in combination with specific amino acids and vitamins, the components have a synergistic effect, so that the pregnancy rate, the litter size and the survival rate of newborn animals of pets are remarkably increased, and meanwhile, the pet feed has good storage stability and use safety.

Description

Technical Field

[0001] This invention belongs to the field of pet nutrition technology, specifically relating to a micronutrient compound additive for improving pet reproductive performance, its preparation method, and its application. Background Technology

[0002] Optimizing pet reproductive performance has always been a crucial research direction in the field of pet nutrition and reproduction. With the increasing status of pets in households and the growing demand for more refined feeding practices, the market has placed higher demands on nutritional supplements that can scientifically, safely, and effectively enhance pet reproductive performance. Trace elements, as key nutrients involved in various physiological metabolisms, play an irreplaceable role in pet reproduction; however, current technologies in this area still have significant limitations.

[0003] Currently available pet reproductive nutritional supplements suffer from the following technical deficiencies: First, their formulations are simplistic and crude, often employing single trace element supplements or basic vitamin-mineral premixes. There is a lack of scientific research on the compatibility and synergistic mechanisms between the various nutrients, resulting in low bioavailability and limited effectiveness. Second, raw material selection and processing are outdated, with inorganic trace elements still dominating, such as common zinc sulfate and copper sulfate. These have low absorption rates and are prone to antagonistic reactions with other components. The application of organic trace elements is often limited to simple addition, failing to fully utilize their biological efficacy through scientific preparation processes. Third, existing products generally exhibit poor stability, easily leading to trace element oxidation, clumping, and decreased solubility during storage, directly impacting product shelf life and efficacy. Fourth, application plans lack specificity, failing to design precise timing, dosage, and cycles for addition based on the physiological characteristics of pet reproduction, significantly diminishing the effectiveness of nutritional intervention.

[0004] It is particularly important to note that current technologies lack sufficient research on the synergistic and antagonistic relationships between trace elements, failing to effectively address the balance issues between key trace elements such as selenium and vitamin E, and zinc and copper. In terms of preparation processes, traditional simple mixing methods are insufficient to guarantee the uniform distribution of trace components and provide inadequate protection for heat-sensitive nutrients (such as vitamin E). Furthermore, existing products often neglect the optimized selection and treatment of carrier materials; the impact of ordinary carriers on the adsorption and stability of trace elements is not adequately considered, leading to significant attenuation of product efficacy during storage and use. Summary of the Invention

[0005] To address the aforementioned problems, this invention discloses a trace element compound additive for improving pet reproductive performance.

[0006] The objective of this invention is achieved through the following technical solution.

[0007] A micronutrient compound additive for improving pet reproductive performance, comprising the following ingredients by weight: 10-20 parts zinc sulfate; Sodium selenite 0.1-0.5 parts; 1-5 parts copper sulfate; 2-8 parts of manganese sulfate; Vitamin E acetate 5-15 parts; 10-20 parts of L-lysine hydrochloride; Taurine 3-8 parts; Methionine 2-6 parts; 1-3 parts of yeast selenium; 30-60 parts zeolite powder; The zeolite powder is activated by acid treatment. The activation method is as follows: the zeolite powder is treated with 1-2 mol / L hydrochloric acid solution at 50-60℃ and stirred for 1-2 hours. After washing until neutral, it is dried at 105-110℃ until the moisture content is ≤5%.

[0008] The above-mentioned scheme constructs a complete reproductive nutrition supplementation system by scientifically proportioning essential trace elements such as zinc, selenium, copper, and manganese, and combining them with vitamin E, amino acids, and functional carriers. Organic and inorganic selenium work synergistically, amino acids enhance metabolic pathways, and activated zeolite powder ensures stability; all components work together to effectively improve reproductive performance.

[0009] Furthermore, in the aforementioned trace element compound additive, the organic selenium content of the yeast selenium is ≥2000 mg / kg, and the particle size is 150-200 mesh. This scheme limits the organic selenium content and particle size range of the yeast selenium to ensure that selenium exists in a highly bioavailable organic form. The fine particle size facilitates uniform dispersion and absorption, resulting in better synergistic effects with other trace elements.

[0010] This invention also discloses a method for preparing the above-mentioned trace element composite additive, comprising the following steps: (1) Pretreatment: The zeolite powder is treated with 1-2 mol / L hydrochloric acid solution at 50-60℃ and stirred for 1-2 hours. After washing until neutral, it is dried at 105-110℃ until the moisture content is ≤5% to obtain activated zeolite powder. (2) First mixing: Zinc sulfate, copper sulfate, manganese sulfate, sodium selenite and yeast selenium are mixed and pulverized through a 100-120 mesh sieve using an air jet mill; (3) Second mixing: Add the mixture obtained in step (2) to a high-speed mixer along with vitamin E acetate, L-lysine hydrochloride, taurine and methionine, and mix for 10-20 minutes at a speed of 200-300 r / min. (4) Third mixing: Add the mixture obtained in step (3) and the activated zeolite powder into a three-dimensional motion mixer and mix for 30-60 minutes to obtain a uniform powder, thus obtaining the trace element composite additive.

[0011] The above scheme adopts a three-step mixing process, which improves the activity of trace elements through air jet pulverization pretreatment, ensures the stability of heat-sensitive components through stepwise mixing, and guarantees uniformity through three-dimensional motion mixing. This process effectively ensures the consistency and stability of the final product quality.

[0012] Furthermore, in the above preparation method, the pulverizing pressure of the air jet mill in step (2) is 0.6-0.8 MPa, and the particle size distribution D90 ≤ 50 μm is controlled. Controlling the air jet pulverizing pressure and particle size distribution ensures that the trace elements reach the optimal particle size range, which not only guarantees the uniformity of mixing but also avoids the dust and agglomeration problems caused by excessively fine powder, thus improving the controllability of the process.

[0013] Furthermore, in the above preparation method, the mixing temperature in step (3) is controlled at 25-35℃, and a double-layer spiral ribbon impeller is used. By controlling the mixing temperature and using a specific impeller, the activity of heat-sensitive components such as vitamin E acetate is protected, while ensuring that all components are fully mixed without dead zones, thus improving the quality stability of the product.

[0014] This invention also discloses the application of the above-mentioned trace element compound additive in the preparation of feed to improve the reproductive performance of pets, for use in the breeding season feed of canine or feline pets, with an addition amount of 0.1-0.5% of the total feed weight. This scheme limits the addition amount and applicable objects, ensuring that the trace element supplementation dosage can meet the special needs of the breeding season while not exceeding the safe range, thus achieving scientific and precise nutritional intervention.

[0015] Furthermore, in the above application, the addition method is to premix it into dry pet food, and the feeding period is from one month before breeding to one month after breeding, feeding twice a day. This plan clearly defines the feeding time and method, covers the critical breeding period, and improves the mother's physical condition, promotes fetal development, and ensures the quality of milk production through continuous nutritional support, forming a complete nutritional management plan for the breeding cycle.

[0016] Furthermore, the aforementioned trace element compound additive, according to animal experiments, increased the conception rate of female dogs by 15-20% and increased the number of puppies by 10-15% after feeding.

[0017] Furthermore, in the above-mentioned trace element composite additive, the trace element dissolution retention rate is ≥95% after being stored at 40°C and 75% relative humidity for 90 days in an accelerated storage test.

[0018] Compared with existing technologies, the present invention has the following advantages and beneficial effects: First, by scientifically proportioning organic and inorganic selenium, and combining it with the synergistic effects of trace elements such as zinc, copper, and manganese, the bioavailability of trace elements is significantly improved. Second, using acid-treated activated zeolite powder as a carrier enhances the stability and uniformity of the product, effectively preventing clumping and nutrient loss. Third, by adding amino acids such as taurine and methionine, a synergistic effect is achieved with trace elements, better meeting the special nutritional needs of pets during the breeding season. Finally, the product of this invention is safe to use, with no adverse reactions even with long-term use. It can effectively increase the pet's conception rate by 15-20%, increase the number of litters by 10-15%, improve the survival rate and birth weight of offspring, and improve the quality of maternal lactation, demonstrating significant economic benefits and application value. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below. However, it should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of the invention. Furthermore, descriptions of well-known structures and technologies are omitted in the following description to avoid unnecessarily obscuring the concept of the invention. All raw materials used in the embodiments of this invention are commercially available.

[0020] The basic diet formula used in the test of this invention Basic dog diet formula: The raw material composition includes 35.0% corn, 25.0% soybean meal, 8.0% fish meal, 10.0% wheat bran, 4.0% vegetable oil, 3.0% premix, 1.5% limestone powder, 1.0% dicalcium phosphate, and 0.5% salt. Nutritional indicators guarantee crude protein ≥22.0%, crude fat ≥8.0%, crude fiber ≤5.0%, calcium 1.0-1.5%, phosphorus 0.8-1.2%, moisture ≤12.0%, lysine ≥1.2%, methionine ≥0.6%, and a metabolizable energy of 3200 kcal / kg. The premix provides vitamins A, D, E, K, and B vitamins, as well as basic trace elements such as iron and iodine, but intentionally excludes the specific proportions of trace elements involved in this invention.

[0021] Basic cat food formula: The raw material composition includes 30.0% chicken meal, 25.0% corn, 15.0% rice, 8.0% fish meal, 6.0% soybean meal, 4.0% vegetable oil, 3.0% premix, 2.5% brewer's yeast, 1.2% limestone powder, 0.8% dicalcium phosphate, and 0.5% salt. Nutritional indicators guarantee crude protein ≥30.0%, crude fat ≥10.0%, crude fiber ≤3.0%, calcium 1.0-1.5%, phosphorus 0.8-1.2%, taurine ≥0.1%, moisture ≤10.0%, and metabolizable energy of 3500 kcal / kg.

[0022] Example 1 A micronutrient compound additive for improving pet reproductive performance, made from the following raw materials in parts by weight: Zinc sulfate 10 parts, sodium selenite 0.1 parts, copper sulfate 1 part, manganese sulfate 2 parts, vitamin E acetate 5 parts, L-lysine hydrochloride 10 parts, taurine 3 parts, methionine 2 parts, yeast selenium 1 part, zeolite powder 30 parts; The zeolite powder is activated by acid treatment. The activation method is as follows: the zeolite powder is treated with 1 mol / L hydrochloric acid solution at 50°C and stirred for 1 hour. After washing until neutral, it is dried at 105°C until the moisture content is ≤5%. The yeast selenium has an organic selenium content of ≥2000 mg / kg and a particle size of 150 mesh.

[0023] The preparation method of the above-mentioned composite additive includes: (1) Pretreatment: The zeolite powder is activated by acid treatment according to the above method to obtain activated zeolite powder; (2) First mixing: Zinc sulfate, copper sulfate, manganese sulfate, sodium selenite and yeast selenium are mixed and pulverized through a 100-mesh sieve using an air jet mill at a pressure of 0.6 MPa, and the particle size distribution D90≤50μm is controlled. (3) Second mixing: Add the mixture obtained in step (2) to a high-speed mixer along with vitamin E acetate, L-lysine hydrochloride, taurine and methionine, and mix at 200 r / min for 10 minutes, controlling the temperature at 25°C; (4) Third mixing: Add the mixture obtained in step (3) and the activated zeolite powder into a three-dimensional motion mixer and mix for a total of 30 minutes to obtain a uniform powder.

[0024] Example 2 A micronutrient compound additive for improving pet reproductive performance, made from the following raw materials in parts by weight: Zinc sulfate 15 parts, sodium selenite 0.3 parts, copper sulfate 3 parts, manganese sulfate 5 parts, vitamin E acetate 10 parts, L-lysine hydrochloride 15 parts, taurine 5 parts, methionine 4 parts, yeast selenium 2 parts, zeolite powder 45 parts. The zeolite powder is activated by acid treatment. The activation method is as follows: the zeolite powder is treated with 1.5 mol / L hydrochloric acid solution at 55°C with stirring for 1.5 hours, washed until neutral, and then dried at 108°C until the moisture content is ≤5%. The yeast selenium has an organic selenium content of ≥2000 mg / kg and a particle size of 180 mesh.

[0025] The preparation method of the above-mentioned composite additive includes: (1) Pretreatment: The zeolite powder is activated by acid treatment according to the above method to obtain activated zeolite powder; (2) First mixing: Zinc sulfate, copper sulfate, manganese sulfate, sodium selenite and yeast selenium are mixed and pulverized through a 110-mesh sieve using an air jet mill at a pressure of 0.7 MPa, and the particle size distribution D90≤50μm is controlled. (3) Second mixing: Add the mixture obtained in step (2) to a high-speed mixer along with vitamin E acetate, L-lysine hydrochloride, taurine and methionine, and mix at 250 r / min for 15 minutes, controlling the temperature at 30°C; (4) Third mixing: Add the mixture obtained in step (3) and the activated zeolite powder into a three-dimensional motion mixer and mix for a total of 45 minutes to obtain a uniform powder.

[0026] Example 3 A micronutrient compound additive for improving pet reproductive performance, made from the following raw materials in parts by weight: 20 parts zinc sulfate, 0.5 parts sodium selenite, 5 parts copper sulfate, 8 parts manganese sulfate, 15 parts vitamin E acetate, 20 parts L-lysine hydrochloride, 8 parts taurine, 6 parts methionine, 3 parts yeast selenium, and 60 parts zeolite powder. The zeolite powder is activated by acid treatment. The activation method is as follows: the zeolite powder is treated with 2 mol / L hydrochloric acid solution at 60°C and stirred for 2 hours. After washing until neutral, it is dried at 110°C until the moisture content is ≤5%. The yeast selenium has an organic selenium content of ≥2000 mg / kg and a particle size of 200 mesh.

[0027] The preparation method of the above-mentioned composite additive includes: (1) Pretreatment: The zeolite powder is activated by acid treatment according to the above method to obtain activated zeolite powder; (2) First mixing: Zinc sulfate, copper sulfate, manganese sulfate, sodium selenite and yeast selenium are mixed and pulverized through a 120-mesh sieve using an air jet mill at a pressure of 0.8 MPa, and the particle size distribution D90≤50μm is controlled. (3) Second mixing: Add the mixture obtained in step (2) to a high-speed mixer along with vitamin E acetate, L-lysine hydrochloride, taurine and methionine, and mix at 300 r / min for 20 minutes, controlling the temperature at 35°C; (4) Third mixing: Add the mixture obtained in step (3) and the activated zeolite powder into a three-dimensional motion mixer and mix for a total of 60 minutes to obtain a uniform powder.

[0028] Comparative Example 1 A trace element compound additive, made from the following raw materials in parts by weight: Zinc sulfate 15 parts, sodium selenite 0.3 parts, copper sulfate 3 parts, manganese sulfate 5 parts, vitamin E acetate 10 parts, L-lysine hydrochloride 15 parts, taurine 5 parts, methionine 4 parts, zeolite powder 45 parts; (excluding yeast selenium) The zeolite powder was not activated by acid treatment; ordinary zeolite powder was used directly.

[0029] The preparation method is the same as in Example 2.

[0030] Comparative Example 2 A trace element compound additive, made from the following raw materials in parts by weight: Zinc sulfate 15 parts, sodium selenite 0.3 parts, copper sulfate 3 parts, manganese sulfate 5 parts, vitamin E acetate 10 parts, L-lysine hydrochloride 15 parts, yeast selenium 2 parts, zeolite powder 45 parts; (excluding taurine and methionine) The zeolite powder was activated by acid treatment, the same method as in Example 2.

[0031] The preparation method is the same as in Example 2.

[0032] Comparative Example 3 A trace element compound additive, made from the following raw materials in parts by weight: Zinc sulfate 15 parts, copper sulfate 3 parts, manganese sulfate 5 parts, vitamin E acetate 10 parts, L-lysine hydrochloride 15 parts, taurine 5 parts, methionine 4 parts, yeast selenium 2 parts, zeolite powder 45 parts; (excluding sodium selenite) The zeolite powder was activated by acid treatment, the same method as in Example 2.

[0033] The preparation method is the same as in Example 2.

[0034] Comparative Example 4 A trace element compound additive, made from the following raw materials in parts by weight: Zinc sulfate 15 parts, sodium selenite 0.3 parts, copper sulfate 3 parts, manganese sulfate 5 parts, L-lysine hydrochloride 15 parts, taurine 5 parts, methionine 4 parts, yeast selenium 2 parts, zeolite powder 45 parts; (excluding vitamin E acetate) The zeolite powder was activated by acid treatment, the same method as in Example 2.

[0035] The preparation method is the same as in Example 2.

[0036] Comparative Example 5 A trace element compound additive, made from the following raw materials in parts by weight: Zinc sulfate 15 parts, sodium selenite 0.3 parts, copper sulfate 3 parts, manganese sulfate 5 parts, vitamin E acetate 10 parts, L-lysine hydrochloride 15 parts, taurine 5 parts, methionine 4 parts, yeast selenium 2 parts, zeolite powder 45 parts. The zeolite powder was not activated by acid treatment.

[0037] The preparation method adopts a traditional single-mixing process, in which all raw materials are added to the mixer at once and mixed for 30 minutes.

[0038] Test Example 1 Test on the effect of improving reproductive performance Objective: To systematically verify the effect of the trace element compound additive of the present invention on improving the reproductive performance of dogs, focusing on key indicators such as mating rate, pregnancy maintenance and litter quality.

[0039] method: Experimental Design and Grouping: Seventy-two healthy, 2-3 year old female dogs (breed: Golden Retrievers) with similar breeding histories were selected and randomly divided into 6 groups of 12 each, based on weight and previous breeding records. The groupings are as follows: Group A: Basal diet + Product from Example 1 (0.3% addition) Group B: Basic diet + Product from Example 2 (0.3% addition) Group C: Basic diet + Comparative Example 1 product (0.3% added) Group D: Basic diet + Comparative Example 3 product (0.3% added) Group E: Basic diet + commercially available general trace element premix (0.3% added) Group F: Basal diet (blank control) Husbandry and Management: All experimental dogs were housed individually under identical environmental conditions (temperature 22±2℃, relative humidity 50-60%, 12h light / 12h darkness). They were fed twice daily (08:00 and 17:00) and provided with free access to water. The experimental period was from 30 days before mating to 30 days postpartum.

[0040] Detection indicators and methods: a. Pregnancy was diagnosed 30 days post-mating using a color Doppler ultrasound (Mindray M9) to calculate the conception rate. b. Record the number of live puppies born to each bitch and calculate the average number of puppies per litter. c. Weigh the puppies within 24 hours of birth using an electronic balance (accuracy 0.1g). d. Record the survival rate of puppies within 30 days of birth. e. Collect milk from the mother dog 24 hours after giving birth and determine its composition.

[0041] Results: See Table 2.

[0042] Conclusion: Examples 1 and 2 showed excellent performance in all reproductive performance indicators, with Example 2 (preferred ratio) showing the best results. Compared with the comparative examples, the conception rate of the example groups increased by 15-20%, the average litter size increased by 1.5-2.0 puppies, the birth weight of puppies increased by more than 15%, and milk quality was significantly improved. The effects of Comparative Example 1 (deficient in yeast selenium) and Comparative Example 3 (deficient in sodium selenite) were significantly reduced, proving that the scientific ratio of organic and inorganic selenium and the synergistic effect of multiple trace elements are key to improving reproductive performance. The product of this invention effectively improves the reproductive performance and milk quality of female dogs by optimizing the form and ratio of trace elements.

[0043] Test Example 2 Product stability and uniformity testing Objective: To systematically evaluate the optimization effect of the preparation process of the present invention on product stability and mixing uniformity.

[0044] method: Sample preparation: Samples of Example 1, Example 2 and Comparative Example 5 (traditional one-time mixing process) were prepared according to the standard process.

[0045] Test items and methods: a. Determination of mixing uniformity: Following the method in GB / T 5918-2008, each sample was randomly sampled 10 times, and the copper content was determined using atomic absorption spectrometry. The coefficient of variation was then calculated. b. Accelerated stability test: The samples were placed in a constant temperature and humidity chamber at 40℃ and 75%RH, and samples were taken at 0, 30, 60, and 90 days. The trace element dissolution rate was determined according to the method in the Chinese Veterinary Pharmacopoeia. c. Clumping assessment: After 90 days of storage, the clumping strength was measured using a texture analyzer, and a sensory evaluation was also conducted. d. Flowability measurement: The angle of repose was measured using a Hall effect flowmeter. Results: See Table 3.

[0046] Conclusion: The three-step mixing process (air jet milling pretreatment, high-speed mixing, and three-dimensional motion final mixing) employed in this invention significantly improves the physical properties of the product. The mixing uniformity (coefficient of variation <5%) in Examples 1 and 2 is significantly better than that of the traditional process (>12%). After a 90-day accelerated test, the dissolution rate retention exceeds 96%, and the angle of repose is less than 40°, indicating that the product has good flowability and is not prone to clumping. These characteristics ensure the quality stability of the product during storage and use, providing a reliable guarantee for practical applications.

[0047] Test Example 3 Application timeliness optimization test Objective: To verify the scientific validity and effectiveness of the specific addition period scheme during the reproductive period proposed in this invention.

[0048] method: Experimental design: 36 breeding female cats (breed: British Shorthair) were selected and randomly divided into 3 groups according to age and weight.

[0049] Group A: Complete cycle group, which continuously added the product of Example 2 (addition amount 0.3%) from one month before breeding to one month after breeding according to the method of this invention. Group B: Short-term addition group, added only during the mating period (7 days before mating to 7 days after mating). Group C: Blank control group, no supplements added. Observation indicators: a. Weigh yourself weekly and record any changes in your body condition. b. Pregnancy diagnosis via ultrasound 25 days after mating. c. Record delivery details, including the number of piglets born and the number of live piglets. d. Measure the kittens' birth weight and weight at 21 days of age. e. Collect blood from the mother cat to measure the levels of reproductive-related hormones. Results: See Table 4.

[0050] Conclusion: The complete reproductive cycle supplementation program (Group A) was significantly superior to the short-term supplementation group (Group B) and the control group (Group C) in all observed indicators. Group A showed a 16.7% increase in conception rate, an average increase of 0.7 kittens per litter, and increases in kitten birth weight and 21-day weight by 17% and 24%, respectively, while progesterone levels remained within a more suitable range. This indicates that improved reproductive performance requires a complete nutritional support cycle. The supplementation program proposed in this invention, covering one month before and one month after breeding, can fully meet the nutritional needs of the mother at each stage of reproduction, ensuring optimal reproductive results.

[0051] Test Example 4 Safety and tolerability assessment Objective: To comprehensively evaluate the long-term safety and animal tolerance of the product of this invention.

[0052] method: Experimental animals: Thirty healthy adult beagles, weighing 8-12 kg, were selected and randomly divided into 3 groups according to their weight.

[0053] Dosage design: Group A: High-dose group, with an addition of 0.5% (1.67 times the recommended dose). Group B: Recommended dosage group, addition amount 0.3% Group C: Blank control group Testing indicators: a. Clinical symptom observation: Observe mental state, appetite, and stool characteristics daily. b. Growth and development indicators: Weigh yourself weekly and calculate average daily weight gain. c. Hematological examination: Blood samples will be collected at the beginning and end of the test for routine blood tests. d. Blood biochemical indicators: Measurement of ALT, AST, ALP, CREAM, BUN, etc. e. Histopathological examination: At the end of the experiment, an autopsy was performed, and liver and kidney tissues were collected for pathological examination. Results: During the 90-day trial, all dogs in all groups maintained good mental condition, normal appetite, and showed no abnormal clinical signs. Weight gain was stable, with no significant difference between the dosage groups and the control group. Blood routine and biochemical indicators were all within the normal physiological range, and histopathological examination revealed no specific lesions related to the additives.

[0054] Conclusion: Long-term use of the product of this invention at the recommended dose (0.3%) and 1.67 times the recommended dose did not produce any adverse effects on the test dogs. All physiological and biochemical indicators were normal, and there were no pathological changes in the tissues and organs, proving that the product has good safety and animal tolerability.

[0055] Test Example 5 Multi-variety applicability verification Objective: To verify the applicability and consistency of the product of this invention in dog breeds of different sizes.

[0056] method: Experimental design: 18 small dogs (Teddy dogs), 18 medium dogs (Corgi dogs), and 18 large dogs (Labrador dogs) were selected and randomly divided into experimental and control groups according to weight within each breed.

[0057] Feeding plan: Experimental group: basal diet + product from Example 2 (addition amount 0.3%) Control group: basal diet Trial period: 30 days before mating to 30 days after parturition Observation indicators: a. Reproductive performance indicators: conception rate, litter size, and birth weight of puppies. b. Maternal condition: postpartum weight loss rate, uterine involution time c. Puppy development: Weaning weight at 28 days old, survival rate Results: See Table 5.

[0058] Conclusion: The product of this invention demonstrated excellent reproductive performance improvement in dog breeds of different sizes. The conception rate in the experimental groups increased by 12-16% across all breeds, the average litter size increased by 0.5-1.0 puppies, the birth weight of puppies increased by 10-15%, and the postpartum weight loss of the mother decreased by 40-50%. This indicates that the product of this invention has broad breed applicability, and its formulation and dosage can meet the specific nutritional needs of dogs of different sizes during the breeding season, demonstrating significant potential for widespread application.

[0059] Test Case Summary: Through the above systematic testing and verification, the product of this invention demonstrates a significant effect in improving pet reproductive performance. Test Example 1 shows that the conception rate of female dogs in the Example 2 group reached 95.8%, with an average litter size of 7.9 puppies, significantly better than the comparative and control groups. Test Example 2 proves that the preparation process of this invention effectively improves the uniformity and stability of the product, with a trace element solubility retention rate of 97.8% after a 90-day accelerated test. Test Example 3 verifies the importance of a complete supplementation regimen during the reproductive period, with the reproductive effect of the complete cycle group being significantly better than that of the short-term supplementation group. Test Example 4 shows that the product still exhibits good safety at 1.67 times the recommended dosage. Test Example 5 confirms that the product is suitable for dog breeds of different sizes, effectively improving reproductive performance in Teddy dogs, Corgis, and Labradors. In summary, the results of these tests demonstrate that this invention, through scientific formulation design and optimized preparation process, achieves synergistic effects among its components, providing an effective technical solution for improving pet reproductive performance.

[0060] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, any changes and modifications made to the embodiments described herein based on the innovative concept of the present invention, or equivalent structural or procedural transformations made using the content of the present invention specification, directly or indirectly applying the above technical solutions to other related technical fields, are all included within the scope of protection of the present invention patent.

Claims

1. A trace element compound additive for improving pet reproductive performance, characterized in that, Raw materials, by weight, include: 10-20 parts zinc sulfate; Sodium selenite 0.1-0.5 parts; 1-5 parts copper sulfate; 2-8 parts of manganese sulfate; Vitamin E acetate 5-15 parts; 10-20 parts of L-lysine hydrochloride; Taurine 3-8 parts; Methionine 2-6 parts; 1-3 parts of yeast selenium; 30-60 parts zeolite powder; The zeolite powder is activated by acid treatment. The activation method is as follows: the zeolite powder is treated with 1-2 mol / L hydrochloric acid solution at 50-60℃ and stirred for 1-2 hours. After washing until neutral, it is dried at 105-110℃ until the moisture content is ≤5%.

2. The trace element composite additive according to claim 1, characterized in that: The yeast selenium has an organic selenium content of ≥2000 mg / kg and a particle size of 150-200 mesh.

3. The method for preparing the trace element composite additive as described in any one of claims 1-2, characterized in that, Includes the following steps: (1) Pretreatment: The zeolite powder is treated with 1-2 mol / L hydrochloric acid solution at 50-60℃ for 1-2 hours by stirring. After washing until neutral, it is dried at 105-110℃ until the moisture content is ≤5% to obtain activated zeolite powder. (2) First mixing: Zinc sulfate, copper sulfate, manganese sulfate, sodium selenite and yeast selenium are mixed and pulverized through a 100-120 mesh sieve using an air jet mill; (3) Second mixing: Add the mixture obtained in step (2) to a high-speed mixer along with vitamin E acetate, L-lysine hydrochloride, taurine and methionine, and mix for 10-20 minutes at a speed of 200-300 r / min. (4) Third mixing: Add the mixture obtained in step (3) and the activated zeolite powder into a three-dimensional motion mixer and mix for 30-60 minutes to obtain a uniform powder, thus obtaining the trace element composite additive.

4. The preparation method according to claim 3, characterized in that: The pulverizing pressure of the air jet mill in step (2) is 0.6-0.8 MPa, and the particle size distribution D90 is controlled to be ≤50 μm.

5. The preparation method according to claim 3, characterized in that: The mixing temperature in step (3) is controlled at 25-35℃, and a double-layer spiral ribbon agitator is used.

6. The application of the trace element compound additive as described in claim 1 in the preparation of feed to improve pet reproductive performance, characterized in that: For use as a supplement in the breeding diet of canine or feline pets, the amount added is 0.1-0.5% of the total weight of the diet.

7. The application according to claim 6, characterized in that: The method of adding is to premix it into dry pet food, and the feeding time is from one month before breeding to one month after breeding, and feeding twice a day.

8. The trace element composite additive according to claim 1, characterized in that: Animal studies have shown that the additive increases the conception rate of female dogs by 15-20% and the number of puppies per litter by 10-15% after feeding.

9. The trace element composite additive according to claim 1, characterized in that: In accelerated storage tests, the additive maintained a trace element dissolution rate of ≥95% after 90 days of storage at 40°C and 75% relative humidity.

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