Balancing liquid for regulating gastric acid of ruminant and preparation method of balancing liquid

Abstract

The invention relates to the technical field of feed additives for ruminants, and particularly discloses equilibrium liquid for regulating gastric acid of ruminants and a preparation method of the equilibrium liquid. The equilibrium liquid is prepared by taking sodium humate as a core raw material through the processes of dissolution, ceramic membrane filtration, activated carbon decoloration, plate and frame filtration and the like. The prepared equilibrium liquid can be added into a ruminant concentrate or daily ration in proportion, the pH value of the rumen of the ruminant can be effectively adjusted, and the neutral steady state of the environment in the rumen is achieved. The equilibrium liquid solves the technical problems that ruminant acidosis or excessive alkalinity in the stomach is easily caused and the intestines and stomach of the ruminant are injured when sodium bicarbonate is used for regulating gastric acid in the prior art, has a pH buffering characteristic, plays a role in regulating pH in an acidic environment, does not take effect when no acidity exists, and is low in addition amount, wide in regulation range and low in cost. The method has no adverse effect on the growth of ruminants, and has practical application value.

Description

Technical Field

[0001] This invention belongs to the field of ruminant feed additive technology, and relates to a balanced liquid for regulating gastric acid in ruminants and its preparation method. Background Technology

[0002] A stable rumen pH level is crucial for the digestion, absorption, growth, and development of ruminants (such as cattle). Excessive rumen acid can easily lead to acidosis, causing decreased feed intake, digestive disorders, and severely impacting farming efficiency. Currently, the mainstream method in the industry for regulating rumen acid is to use baking soda (sodium bicarbonate). This is achieved by adding it to concentrates or allowing ruminants free access to neutralize the rumen pH level, thus preventing acidosis.

[0003] Using baking soda to regulate stomach acid has significant technical drawbacks: First, baking soda is quite harmful to the gastrointestinal tract of ruminants, and long-term or improper use can affect their normal growth. Second, the feeding method is difficult to control. If ruminants are not allowed to eat freely, their stomach acid cannot be neutralized in time when it is high, which can easily lead to acidosis. If they eat too much freely, it will cause the stomach of the ruminants to become too alkaline, which will also disrupt their digestive function and have an adverse effect on their growth.

[0004] To completely overcome the technical bottlenecks of baking soda in practical applications, such as gastrointestinal damage caused by its strong alkalinity and feeding difficulties due to its delayed response, this invention introduces sodium humate as a novel buffer. Unlike the simple acid-base neutralization of baking soda, sodium humate utilizes four core mechanisms—physical adsorption, chemical buffering, microbial regulation, and mucosal protection—to synergistically block the vicious cycle of rumen acidosis and stabilize rumen pH and function. Its unique chemical buffering mechanism is key to precise regulation: since humic acid is a weak organic acid, and sodium humate is its sodium salt, when the rumen pH is too low, it balances HA according to the reaction. H + +A The system will spontaneously move to the left to consume excess H. + This directly neutralizes excess lactic acid and volatile acids (VFA); furthermore, the large number of carboxyl groups (-COOH) and phenolic hydroxyl groups (-OH) in sodium humate molecules can be neutralized by highly efficient H+ ions. + Na + The ability to exchange gases allows for bidirectional pH stability. Therefore, developing a gastric acid regulating product that can precisely adjust the rumen pH of ruminants without causing gastrointestinal damage and with easily controllable dosage has become an urgent technical problem to be solved in the field of ruminant farming.

[0005] To address the above problems, this invention proposes a balanced solution for regulating gastric acid in ruminants and its preparation method. Summary of the Invention

[0006] To address the aforementioned deficiencies in the existing technology, the present invention aims to provide a balanced solution for regulating gastric acid in ruminants and its preparation method, thereby solving the problems that sodium bicarbonate regulation of gastric acid can easily cause acidosis / excessive alkalinity in the stomach and damage the gastrointestinal tract of ruminants, and achieving stable regulation of rumen pH in ruminants.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A method for preparing a balanced solution for regulating gastric acid in ruminants includes modification with sodium humate, filtration using ceramic and nanofiltration membranes, and gradient temperature decolorization treatment, specifically: S1. Prepare an alkaline solution and a sodium humate solution for later use; S2. Modification of sodium humate: Sodium humate is modified with an alkaline solution to obtain sodium humate solution A; the pH of sodium humate solution A is 12-13. S3. Sodium humate solution A is filtered through a ceramic membrane and a nanofiltration membrane to remove insoluble matter, resulting in black filtrate B. S4. Perform 1-2 decolorization treatments on the above black filtrate B, and then filter it through a plate and frame filter to obtain a colorless and transparent equilibrium solution.

[0008] Preferably, the mass fraction of the alkaline solution in step S1 is 35-55%, and the mass fraction of the sodium humate solution A is 20-30%.

[0009] Preferably, the raw material used for the alkaline solution is at least one of KOH, Na2CO3 and NaOH.

[0010] Preferably, the specific steps of filtering in step S3 are as follows: S301. The sodium humate solution A described in claim 1 is first filtered through a ceramic membrane with a pore size of 0.1~0.5μm to obtain a black liquid to be treated; S302. The above-mentioned black liquid to be treated is filtered through a 100~500Da nanofiltration membrane to obtain black filtrate B; Preferably, the specific steps of the decolorization process in step S4 are as follows: S401. Add adsorbent to the black filtrate B as described in claim 1, stir evenly, and obtain a light yellow solution C1. S402. The above pale yellow solution C1 is filtered through a plate and frame filter to obtain pale yellow solution C2; S403. Repeat steps S401 to S402 to obtain a colorless and transparent equilibrium liquid.

[0011] Preferably, in step S401, the stirring speed is 80-120 rpm, the stirring temperature is 80-90℃, and the stirring time is 30-45 min; in step S403, the stirring speed is 80-120 rpm, the stirring temperature is 40-50℃, and the stirring time is 10-20 min.

[0012] Preferably, the adsorbent has a mass fraction of 1.5-5%; wherein, The mass fraction of the adsorbent mentioned in step S401 is 3-5%; The mass fraction of the adsorbent mentioned in step S403 is 1.5~3%.

[0013] Preferably, the adsorbent is at least one of activated carbon, attapulgite, and activated clay.

[0014] A balanced solution for regulating gastric acid in ruminants, said balanced solution being prepared by the preparation method according to any one of claims 1 to 8.

[0015] Preferably, the balancing solution is one of the components of a gastric buffer, and the mass ratio of the balancing solution to the gastric buffer is 1:115~135.

[0016] Compared with the prior art, the present invention has the following beneficial effects: (1) The balancing solution of the present invention uses sodium humate as the core and has unique pH buffering properties. It only plays a role in raising pH when the rumen of ruminants is acidic, and does not play a role when there is no acidity. It can accurately stabilize the rumen pH in the normal range of 6.5~7.0. It achieves bidirectional pH stabilization through the ion exchange of carboxyl and phenolic hydroxyl groups, which can effectively prevent and alleviate acidosis. It is not a simple acid-base neutralization.

[0017] (2) The preparation process of the present invention only includes conventional steps such as dissolution, modification, filtration, and gradient temperature decolorization. The process parameters are clear and highly controllable. The "ceramic membrane + nanofiltration membrane" dual filtration process can effectively remove insoluble substances, and the gradient temperature secondary decolorization process can improve the utilization rate of effective components, ensure the stability of product adjustment effect, and meet the needs of large-scale industrial production.

[0018] (3) The amount of balanced liquid added in this invention is low, and the ratio of compounding with gastric buffer is only 1:115~135; it can stabilize the rumen environment, ensure the activity of rumen microorganisms, significantly increase the daily feed intake of ruminants, and maintain the fecal pH in the appropriate range of 7.0~8.0, effectively improving their growth performance; it can be used as a preferred feed additive for regulating gastric acid in ruminants, and has broad prospects for promotion in the field of ruminant breeding. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic flowchart of a method for preparing a balancing solution for regulating gastric acid in ruminants, provided in an embodiment of the present invention. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0022] To achieve the above-mentioned objectives of the present invention, the technical solution of the present invention will be further described in detail below, but the scope of protection of the present invention is not limited to the following description.

[0023] A method for preparing a balanced solution for regulating gastric acid in ruminants includes modification with sodium humate, filtration with ceramic and nanofiltration membranes, gradient temperature decolorization treatment, and finished product packaging.

[0024] The modification of the sodium humate involves steps S1-S2 as described in claim 1, specifically: S1. Prepare an alkaline solution for later use. The solvent used is deionized water. In some embodiments, the alkaline solution is a 35-40% NaOH aqueous solution, while in other embodiments, the alkaline solution is a 50-55% KOH aqueous solution.

[0025] S2. Dissolve 8-12g of sodium humate in 240mL of deionized water and stir at 40-60℃ until completely dissolved to obtain sodium humate solution A; adjust the pH of sodium humate solution A to 12-13 with an alkaline solution. The ceramic membrane filtration and nanofiltration involve step S3 as described in claim 1, specifically: S3. The sodium humate solution A is filtered through a ceramic membrane and a nanofiltration membrane to remove insoluble matter, yielding a black filtrate B, specifically: S301. Filter sodium humate solution A through a 0.1~0.5μm ceramic membrane to remove large undissolved particles and obtain a black liquid to be treated. S302. The above black liquid to be treated is filtered through a 100~500Da nanofiltration membrane to remove small insoluble particles and obtain black filtrate B. The gradient temperature decolorization process involves step S4 as described in claim 1, specifically: S4. Perform 1-2 decolorization treatments on the black filtrate B described in step S3 to obtain a colorless and transparent equilibrium solution; the first decolorization treatment involves steps S401-S402 of claim 5, specifically: S401. Add 3-5% adsorbent to black filtrate B, stir at 80-120 rpm and 80-90℃ for 30-45 min to obtain pale yellow solution C1. In some embodiments, the adsorbent is activated carbon or activated clay, while in other embodiments, the adsorbent is a composite adsorbent, wherein the composite adsorbent is attapulgite and activated carbon in a mass ratio of 1:2, or activated clay and activated carbon in a mass ratio of 1:3, or activated clay and attapulgite in a mass ratio of 1:1.

[0026] S402. The above pale yellow solution C is filtered through a plate and frame filter to obtain pale yellow solution C2; The second decolorization process involves step S403 of claim 2, specifically: S403. Repeat steps S401 to S402 above, keeping the stirring rate constant, and change the stirring temperature and time, specifically stirring at 40 to 50°C for 10 to 20 minutes to obtain a colorless and transparent equilibrium liquid. The type of adsorbent is the same as in step S401, and its mass is 25 to 50% of the adsorbent in step S401.

[0027] S5. The above-mentioned colorless and transparent balancing solution and gastric buffer are mixed at a ratio of 1:115~135 and used to feed ruminants (the present invention takes cattle as an example).

[0028] Example 1 S1. Prepare a 40% NaOH solution for later use; S2. Dissolve 10g of sodium humate in 240mL and stir at 50℃ until completely dissolved to obtain sodium humate solution A. Adjust the pH of sodium humate solution A to 13 with 40% NaOH solution. S3. The above sodium humate solution A is filtered through a ceramic membrane and a nanofiltration membrane to obtain black filtrate B, specifically: S301. Sodium humate solution A is filtered through a 0.2μm ceramic membrane to remove large undissolved particles, resulting in a black liquid to be treated. S302. The above black liquid to be treated is filtered through a 200Da nanofiltration membrane to remove small insoluble particles and obtain a black filtrate. S4. Add activated carbon to the above black filtrate B to obtain sodium humate equilibrium solution; specifically: S401. Add 0.4g of activated carbon to the above black filtrate and stir for 40min to obtain a pale yellow solution C1. S402. After filtering the above pale yellow solution C1 through a plate and frame filter, a pale yellow solution C2 is obtained. S403. Add 0.2g of activated carbon to the pale yellow solution C2, stir for 30min, and then filter through a plate and frame filter to obtain a colorless and transparent equilibrium solution. S5. Mix the above colorless and transparent equilibration solution with gastric buffer at a ratio of 1:125.

[0029] Example 2 S1. Prepare a 35% NaOH solution for later use; S2. Dissolve 8g of sodium humate in 240mL of deionized water and stir at 40℃ until completely dissolved to obtain sodium humate solution A. Adjust the pH of sodium humate solution A to 12.5 with 35% NaOH solution. S3. The above sodium humate solution A is filtered through a ceramic membrane and a nanofiltration membrane to obtain black filtrate B, specifically: S301, sodium humate solution A is filtered through a 0.5μm ceramic membrane to remove large undissolved particles, resulting in a black liquid to be treated; S302. The above black liquid to be treated is filtered through a 500Da nanofiltration membrane to remove small insoluble particles and obtain black filtrate B. S4. Add a composite adsorbent to the black filtrate B to obtain a sodium humate equilibrium solution; specifically: S401. Add 0.2g of activated carbon and 0.1g of attapulgite compound to the above black filtrate B, stir for 30min to obtain a light yellow solution C1. S402. The above pale yellow solution C1 is filtered through a plate and frame filter to obtain pale yellow solution C2; S403. Add 0.75g of activated carbon and 0.25g of attapulgite as a compound adsorbent to the pale yellow solution C2, stir for 30min, and obtain a colorless and transparent equilibrium solution after plate and frame filtration. S5. Mix the above colorless and transparent equilibration solution with gastric buffer at a ratio of 1:115.

[0030] Example 3 S1. Prepare a 55% KOH solution for later use; S2. Dissolve 12g of sodium humate in 240mL of deionized water and stir at 60℃ until completely dissolved to obtain sodium humate solution A. Adjust the pH of sodium humate solution A to 12.8 with 55% KOH solution. S3. The above sodium humate solution A is filtered through a ceramic membrane and a filter membrane to obtain black filtrate B, specifically: S301. Sodium humate solution A is filtered through a 0.2μm ceramic membrane to remove large undissolved particles, resulting in a black liquid to be treated. S302. The above black liquid to be treated is filtered through a 200Da nanofiltration membrane to remove small insoluble particles and obtain black filtrate B. S4. The black filtrate B is subjected to a second decolorization treatment to obtain a sodium humate equilibrium solution; specifically: S401. Add 0.5g of activated clay to the above black filtrate B and stir for 45min to obtain a light yellow solution C1. S402. After filtering the above pale yellow solution C1 through a plate and frame filter, a pale yellow solution C2 is obtained. S403. Add 0.25g of activated clay to the pale yellow solution C2, stir for 40min, and filter through a plate and frame filter to obtain a colorless and transparent equilibrium solution. S5. Mix the above colorless and transparent equilibration solution with gastric buffer at a ratio of 1:135.

[0031] Example 4 S1. Prepare a 50% KOH solution for later use; S2. Dissolve 10g of sodium humate in 240mL of deionized water and stir at 45℃ until completely dissolved to obtain a 4% sodium humate solution A. Adjust the pH of sodium humate solution A to 12 with 50% KOH solution.

[0032] S3. The above sodium humate solution A is filtered through a ceramic membrane and a filter membrane to obtain black filtrate B, specifically: S301. Sodium humate solution A is filtered through a 0.2μm ceramic membrane to remove large undissolved particles, resulting in a black liquid to be treated. S302. The above black liquid to be treated is filtered through a 100Da nanofiltration membrane to remove small insoluble particles and obtain black filtrate B. S4. The black filtrate B is subjected to a second decolorization treatment to obtain a sodium humate equilibrium solution; specifically: S401. Add 0.3g of activated carbon and 0.1g of activated clay as a compound adsorbent to the black filtrate B, stir for 38 minutes, and obtain a light yellow solution C1 by plate and frame filtration. S402. After filtering the above pale yellow solution C1 through a plate and frame filter, a pale yellow solution C2 is obtained. S403. Add 0.1g of activated carbon and 0.2g of activated clay as a compound adsorbent to the pale yellow solution C2, stir for 35min, and obtain a colorless and transparent equilibrium solution by plate and frame filtration. S5. Mix the above colorless and transparent equilibration solution with gastric buffer at a ratio of 1:125.

[0033] Comparative Example 1 Compared with Example 1, the equilibration fluid was removed.

[0034] Comparative Example 2 Compared with Example 1, 10g of sodium humate was dissolved in 240mL of deionized water to obtain sodium humate solution A. Afterwards, no filtration or decolorization was performed; all other operations and steps were the same as in Example 1, specifically: S1. Prepare a 40% NaOH solution for later use; S2. Dissolve 10g of sodium humate in 240mL and stir at 50℃ until completely dissolved to obtain sodium humate solution A. Adjust the pH of sodium humate solution A to 13 with 40% NaOH solution. S3. Mix the above sodium humate solution A with the gastric buffer at a ratio of 1:125.

[0035] Comparative Example 3 Compared with Example 1, the equal amount of baking soda was used to replace the equilibration liquid, and the rest of the operation was exactly the same as in Example 1.

[0036] Comparative Example 4 Compared with Example 1, only one decolorization treatment was performed, specifically: S1~S3 and S5 are completely identical to those in Example 1; S4. Add activated carbon to the above-mentioned black liquid to be treated to obtain a sodium humate equilibrium solution; specifically: S401. Add 0.4g of activated carbon to the above black liquid to be treated, stir for 40min, and obtain a light yellow solution C1. S402. The above pale yellow solution C1 is filtered through a plate and frame filter to obtain the second equilibrium solution.

[0037] Experimental Section: The normal pH range of the bovine rumen is typically between 6.5 and 7.0. This value is a key indicator for maintaining rumen microbial activity, feed digestibility, and ruminant health. When the pH falls below 5.5, rumen acidosis may occur. This experiment first conducted a simulated rumen environment experiment to verify the pH regulation capability of the balanced solution. Based on the results of the simulation experiment, an animal feeding experiment was carried out to examine the actual impact of the balanced solution on bovine growth performance and the digestive tract environment.

[0038] ① Simulated rumen environment experiment Experimental conditions: McDougall's buffer (artificial saliva) was used as the base solution to simulate the anaerobic and isothermal environment of the bovine rumen: the temperature was maintained at 39±0.5℃, CO2 was bubbled into the buffer solution until saturation was achieved to form a carbonate-bicarbonate buffer system, and the basic pH was stabilized at 6.0~6.8; lactic acid was added to adjust the pH of the base solution to 5.0~5.5 to simulate the bovine rumen acidosis state.

[0039] Experimental Methods: A total of 7 groups were set up, including a blank group (acidosis simulation solution only), Examples 1-4, and Comparative Examples 1-3, with 3 replicates in each group. All groups included the acidosis simulation solution and the product prepared according to the corresponding examples or comparative examples. The reagents of each experimental group were thoroughly mixed and allowed to stand at a constant temperature of 39±0.5℃. The pH value of the solution was measured at 0 min, 5 min, 15 min, 30 min, 60 min, 90 min, and 120 min.

[0040] The experimental results are shown in Table 1 below.

[0041] The pH of the acidosis simulation solution in the blank group remained at 5.0-5.2 throughout the experiment without significant changes. In Examples 1-4, after the addition of the equilibration solution, the pH showed a slow upward and stable trend. The pH was 5.1 in the initial stage, slowly rising to 6.4-6.6 within 30 minutes, and stabilizing at 6.5-6.8 after 60 minutes. It remained stable in this range for the next 90 minutes without any excessive alkalinity or drop, and was completely within the normal pH range of bovine rumen (6.5-7.0). Moreover, there was no excessive alkalinity or drop throughout the experiment, demonstrating the core characteristics of precise buffering and gentle regulation, and achieving precise buffering and regulation of rumen pH.

[0042] Comparative Example 3 showed a rapid and persistently alkaline pH increase after the addition of baking soda, reaching 7.8 within 5 minutes and stabilizing at 7.8-7.9, far exceeding the normal rumen pH. This demonstrates the lack of buffering effect of baking soda and its tendency to cause excessive alkalinity in the stomach. Comparative Example 2 (without filtration and decolorization) only slightly increased the pH, reaching a maximum of 5.8, which failed to reach the normal rumen range, and subsequently dropped slightly. This proves that the filtration and decolorization process is crucial for the balancing solution to exert its regulatory effect. Comparative Example 4 (one-time decolorization) saw the pH rise to a maximum of 6.1. Although it had a regulatory effect, it did not reach the normal range, demonstrating that the secondary gradient temperature decolorization process can improve the utilization rate of the effective components in the balancing solution and ensure the regulatory effect.

[0043] ② Animal feeding experiment: Taking cattle as an example, 5 calves with a weight range of 110±5kg were selected (5 calves were selected in each example). The colorless and transparent balancing solution prepared in Examples 1 to 4 was compounded with the gastric buffer in a certain proportion to obtain a gastric buffer containing the balancing solution. The gastric buffer was added to the feed for feeding (the feed used in this invention is a compound feed, that is, roughage: concentrate = 7:3). The pH of the feces was measured at four weight ranges: calves (110±5kg), medium cattle (240±5kg), large cattle (420±5kg), and slaughter cattle (600±5kg), and the average pH value was calculated.

[0044] Methods for testing fecal pH: Take a fresh fecal sample 2-4 hours after the cattle have eaten, mix it with distilled water at a ratio of 1:5, let it stand for 10 minutes, and take the supernatant to measure the pH with a pH meter; in addition, record the feed intake of each cow every day and calculate the average daily feed intake.

[0045] The experimental results are shown in Table 2 below.

[0046] As can be seen from the table above, the fecal pH of cattle in Examples 1 to 4 was stable at 7.0 to 8.0, which is neutral to slightly alkaline, indicating that the balanced solution prepared by the present invention can accurately and stably regulate the pH of the digestive tract of ruminants.

[0047] The daily feed intake of groups 1-4 (9.0-9.3 kg / head / day) was significantly higher than that of control group 3. This is because the balanced solution stabilized the rumen environment, ensured the activity of rumen microorganisms, and improved feed digestion and absorption efficiency. The alkaline environment caused by sodium bicarbonate in control group 3 disrupted microbial fermentation, resulting in lower growth performance indicators than those of the groups in the examples.

[0048] The experimental data in Tables 1 and 2 show that the pH-regulating solution of this invention has a significant effect on pH adjustment. Compared with traditional baking soda, it has buffering properties and can play a role in raising the pH in the acidic environment of the rumen, stabilizing the rumen pH in the normal range of 6.5 to 7.0. This solves the problem of acidosis and avoids excessive alkalinity in the stomach, fundamentally overcoming the technical defects of baking soda regulation.

[0049] Secondly, the present invention adopts a dual filtration process of "ceramic membrane + nanofiltration membrane" to effectively remove insoluble substances in sodium humate, and the gradient temperature secondary decolorization process improves the utilization rate of effective components; the process of decolorization without filtration and only one decolorization will greatly reduce the adjustment effect of the equilibrium liquid and cannot meet the needs of practical applications.

[0050] Finally, the balanced solution of this invention has a significant effect on improving the growth performance of ruminants. A low addition amount can stabilize the rumen environment, ensure the activity of rumen microorganisms, improve feed digestion and absorption efficiency, and significantly increase the feed intake of cattle. Moreover, long-term feeding has no adverse effects, and the rumen environment remains stable. In contrast, long-term feeding of sodium bicarbonate will cause the rumen to become too alkaline and disrupt digestive function.

[0051] Conclusion: Baking soda (NaHCO3) has a rapid neutralizing effect, but its effect is short-lived, it easily causes rumen "alkali-acid rebound" and affects salivary buffering; while sodium humate has a slow, long-lasting, bidirectional buffering effect, which is more fundamental and stable. Therefore, sodium humate is not a simple "neutralizer", but a comprehensive regulator of the rumen internal environment, with multiple protections, preventing and alleviating rumen acidosis from the root.

[0052] Therefore, the balancing solution of this invention has practical application value. The preparation process uses industry-standard equipment, the steps are easy to operate and highly controllable, and it can achieve large-scale industrial production. At the same time, the dosage is low, the regulating effect is stable, and it does not cause gastrointestinal damage to ruminants. It can replace sodium bicarbonate as the preferred feed additive for regulating gastric acid in ruminants and has broad application prospects in the field of ruminant breeding.

[0053] Finally, it should be noted that although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the present invention.

Claims

1. A method for preparing a balanced solution for regulating gastric acid in ruminants, characterized in that, This includes modification of sodium humate, filtration using ceramic and nanofiltration membranes, and gradient temperature decolorization treatment, specifically: S1. Prepare an alkaline solution and a sodium humate solution for later use; S2. Modification of sodium humate: Sodium humate was modified with an alkaline solution to obtain sodium humate solution A. The pH of the sodium humate solution A is 12-13; S3. Ceramic membrane and nanofiltration membrane filtration: Sodium humate solution A is filtered through a ceramic membrane and a nanofiltration membrane to remove insoluble matter, resulting in black filtrate B; S4. Perform 1-2 decolorization treatments on the above black filtrate B, and then filter it through a plate and frame filter to obtain a colorless and transparent equilibrium solution.

2. The method for preparing a balanced solution for regulating gastric acid in ruminants according to claim 1, characterized in that, The mass fraction of the alkaline solution in step S1 is 35-55%, and the mass fraction of the sodium humate solution A is 20-30%.

3. The method for preparing a balanced solution for regulating gastric acid in ruminants according to claim 2, characterized in that, The raw material used for the alkaline solution is at least one of KOH, Na2CO3 and NaOH.

4. The method for preparing a balanced solution for regulating gastric acid in ruminants according to claim 1, characterized in that, The specific steps for filtering in step S3 are as follows: S301. The sodium humate solution A described in claim 1 is first filtered through a ceramic membrane with a pore size of 0.1~0.5μm to obtain a black liquid to be treated; S302. The above-mentioned black liquid to be treated is filtered through a 100~500Da nanofiltration membrane to obtain black filtrate B.

5. The method for preparing a balanced solution for regulating gastric acid in ruminants according to claim 4, characterized in that, The specific steps of the decolorization process described in step S4 are as follows: S401. Add adsorbent to the black filtrate B as described in claim 1, stir evenly, and obtain a light yellow solution C1. S402. The above pale yellow solution C1 is filtered through a plate and frame filter to obtain pale yellow solution C2; S403. Repeat steps S401 to S402 to obtain a colorless and transparent equilibrium liquid.

6. The method for preparing a balanced solution for regulating gastric acid in ruminants according to claim 5, characterized in that, The stirring speed in step S401 is 80~120 rpm, the stirring temperature is 80~90℃, and the stirring time is 30~45 min; the stirring speed in step S403 is 80~120 rpm, the stirring temperature is 40~50℃, and the stirring time is 10~20 min.

7. The method for preparing a balanced solution for regulating gastric acid in ruminants according to claim 6, characterized in that, The adsorbent has a mass fraction of 1.5-5%; wherein, The mass fraction of the adsorbent mentioned in step S401 is 3-5%; The mass fraction of the adsorbent mentioned in step S403 is 1.5~3%.

8. The method for preparing a balanced solution for regulating gastric acid in ruminants according to claim 7, characterized in that, The adsorbent is at least one of activated carbon, attapulgite, and activated clay.

9. A balancing solution for regulating gastric acid in ruminants, characterized in that, The balanced solution is prepared by the method for preparing a balanced solution for regulating gastric acid in ruminants as described in any one of claims 1 to 8.

10. The balancing solution for regulating gastric acid in ruminants according to claim 9, characterized in that, The balanced solution is one of the components of the gastric buffer, and the mass ratio of the balanced solution to the gastric buffer is 1:115~135.

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