Nutritional food containing small molecule active peptide and preparation method thereof

Abstract

The invention discloses a nutritional food containing small-molecule active peptides, and relates to the technical field of health-care foods, and the nutritional food is composed of the following components in parts by weight: 15-35 parts of deer blood small-molecule active peptides, 10-30 parts of rumex hanus concentrated extract, 15-40 parts of whey protein, 5-20 parts of phycocyanin, 3-15 parts of collagen peptides, and 2-10 parts of glucan. The preparation method of the nutritional food containing the small-molecule active peptide mainly comprises three key steps of preparation of the deer blood small-molecule active peptide, preparation of a rumex hanus concentrated extract and mixing preparation. Through a special preparation process, macromolecular nutrient substances are converted into micromolecular forms, such as deer blood micromolecular active peptides and low-molecular-weight collagen peptides, the bioavailability of the micromolecular nutrient substances is greatly improved, the micromolecular nutrient substances are more easily digested and absorbed by a human body, and compared with traditional macromolecular nutrient foods, the nutritional food has the advantages that the nutritional effect is good; the nutritious food disclosed by the invention can be absorbed and utilized by a human body more quickly.

Description

Technical Field

[0001] This invention relates to the field of health food technology, specifically to a nutritional food containing small molecule active peptides and its preparation method. Background Technology

[0002] With increasing health awareness, the demand for nutritional foods is becoming more diversified and sophisticated. Traditional nutritional foods have many shortcomings in terms of the integrity of nutrients, absorption and utilization rates, and functional characteristics. On the one hand, common nutritional foods often focus only on supplementing a single type of nutrient, such as simply supplementing protein or vitamins, which is insufficient to meet the body's need for the synergistic effect of multiple nutrients. On the other hand, technological limitations in food processing make it easy for nutrients to be lost or inactivated during processing, reducing the nutritional value of the product. For example, in some protein-based nutritional products, large protein molecules are not easily digested and absorbed by the body, and the high temperatures and other conditions during processing can cause protein denaturation, affecting its biological activity. In addition, existing nutritional foods are not ideal in addressing specific health needs of the human body, such as enhancing immunity, anti-oxidation, and repairing damaged cells.

[0003] The invention disclosed in CN116548623A presents a method for preparing an oral liquid containing deer blood polypeptides with immunomodulatory functions. This method improves the hydrolysis effect by using neutral and acidic proteases for enzymatic hydrolysis, increases the degree of hydrolysis of the deer blood polypeptides, and addresses the fishy smell problem by adding β-cyclodextrin and yeast. It also solves the product instability problem by adding a complex colloid. However, this product mainly uses deer blood polypeptides as the core ingredient, supplemented with a small amount of excipients, resulting in a relatively simple nutritional composition. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a nutritional food containing small molecule active peptides and its preparation method, thus solving the problems of existing technologies.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solution: a nutritional food containing small molecule active peptides, which, by weight, consists of 15-35 parts deer blood small molecule active peptides, 10-30 parts concentrated extract of edible leaves, 15-40 parts whey protein, 5-20 parts phycocyanin, 3-15 parts collagen peptides, and 2-10 parts glucan.

[0006] Preferably, the average molecular weight of the deer blood small molecule active peptide is between 100 and 800 Daltons.

[0007] Preferably, the concentration ratio of the concentrated extract of the leafwort is 15-40:1.

[0008] Preferably, the molecular weight of the collagen peptide is between 200 and 1000 Daltons.

[0009] This invention also discloses a method for preparing a nutritional food containing small molecule active peptides, comprising the following steps: Step 1: Dry and grind fresh deer blood into deer blood powder. Mix deer blood powder with distilled water at a weight ratio of 1:8 to 1:20. Sonicate at 120-180W for 25-50 minutes and then oscillate at a constant temperature for 70-110 minutes to obtain a deer blood solution. Step 2: Add acidic protease to the deer blood solution, adjust the pH to 2.2-2.8 with 1.0 mol / L HCl, and enzymatically hydrolyze in a constant temperature shaking water bath at 37-62℃ for 1.5-7.0 h, maintaining pH stability during the enzymatic hydrolysis process; Step 3: Adjust the pH to 7.2-7.8 with 1.0 mol / L NaOH, add neutral protease, and enzymatically hydrolyze in a constant temperature shaking water bath at 37-62℃ for 1.5-7.0 h, maintaining pH stability during the enzymatic hydrolysis. After enzymatic hydrolysis, inactivate the enzyme at 92-98℃ using a magnetic stirrer for 8-25 min, cool, and centrifuge at 8200-9300 rpm for 12-25 min to extract the supernatant. Step 4: Add β-cyclodextrin and yeast to the supernatant sequentially for deodorization. The mass ratio of β-cyclodextrin to deer blood small molecule active peptides is 2.5%-3.5%. Deodorize at 32-55℃ for 40-100 min. The mass ratio of yeast to deer blood small molecule active peptides is 0.8%-1.5%. Deodorize at 32-45℃ for 40-100 min. Centrifuge and collect the supernatant. Pre-freeze for 15-40 h and then freeze-dry to obtain deer blood small molecule active peptide freeze-dried powder. Step 5: Wash the edible leaves, dry them at low temperature until the moisture content is less than 8%, pulverize them through a 60-100 mesh sieve, extract them 2-4 times at 50-80℃ with water or ethanol as solvent, each time for 1-3 hours, combine the extracts, filter them, concentrate them under reduced pressure, concentrate them again to obtain a fluid extract, spray dry them, and pulverize them through an 80-120 mesh sieve to obtain the concentrated edible leaves extract. Step Six: Mix deer blood small molecule active peptide freeze-dried powder, edible leaf grass concentrated extract, whey protein, phycocyanin, collagen peptide, and dextran in proportion. Flavoring agents and stabilizers may be added. Add an appropriate amount of purified water and stir evenly to prepare the corresponding dosage form.

[0010] Preferably, the mass of the acidic protease is 0.4%-0.8% of the deer blood powder, and the mass ratio of the neutral protease to the acidic protease is 1-4:1.

[0011] Preferably, the low-temperature drying temperature of the edible grass is 40-60℃.

[0012] Preferably, when the prepared formulation is an oral liquid, it is filtered through a 0.22μm filter membrane, filled and sterilized; when the prepared formulation is a solid beverage, it is dried, granulated and packaged.

[0013] Preferably, the flavoring agent is one or more of sweeteners and acidifiers, and the stabilizer is one or more of pectin and sodium carboxymethyl cellulose.

[0014] Beneficial effects This invention provides a nutritional food containing small molecule active peptides and its preparation method. Compared with the prior art, it has the following advantages: 1. This nutritional food containing small molecule active peptides contains a variety of essential nutrients, including small molecule active peptides, proteins, amino acids, vitamins, and minerals, providing comprehensive nutritional support. The synergistic effect of deer blood small molecule active peptides, phycocyanin, and glucan effectively regulates the immune system. The antioxidants in the concentrated extract of phycocyanin and phycocyanin can eliminate free radicals in the body and reduce oxidative stress damage to cells. Whether it's adolescents in their growth and development stage, middle-aged and elderly people who need to enhance their immunity, or those pursuing a healthy lifestyle, everyone can obtain rich nutrition from this nutritional food, meeting the nutritional needs of different groups.

[0015] 2. The preparation method of this nutritional food containing small molecule active peptides uses a special preparation process to convert macromolecular nutrients into small molecule forms, such as deer blood small molecule active peptides and low molecular weight collagen peptides. The bioavailability of these small molecule nutrients is greatly improved, making them easier for the human body to digest and absorb. Compared with traditional macromolecular nutritional foods, the nutritional food of this invention can be absorbed and utilized by the human body more quickly, providing timely nutritional supplementation. During the preparation process, the taste and texture of the food are improved by reasonably adding flavoring agents and stabilizers. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the method flow of the present invention. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] See also Figure 1 The present invention provides the following three technical solutions: First implementation method: A nutritional food containing small molecule active peptides, which, by weight, consists of 15-35 parts deer blood small molecule active peptides, 10-30 parts concentrated extract of edible leaves, 15-40 parts whey protein, 5-20 parts phycocyanin, 3-15 parts collagen peptides, and 2-10 parts glucan.

[0019] Deer blood small molecule bioactive peptides: As one of the core components, deer blood small molecule bioactive peptides are extracted from deer blood through specific enzymatic hydrolysis technology. Their average molecular weight is controlled between 100-800 Daltons, a range that gives them excellent bioactivity and absorption. They contain various bioactive components that can participate in the body's immune regulation process and enhance the body's resistance. For example, by stimulating the activity of immune cells, they promote the proliferation and differentiation of immune cells, thereby improving the body's immunity. Simultaneously, deer blood small molecule bioactive peptides also have the function of promoting cell repair and regeneration, aiding in the recovery of damaged tissues.

[0020] Concentrated extract of Eclipta prostrata: Eclipta prostrata is rich in various nutrients, such as abundant protein, multiple amino acids, vitamins (e.g., vitamin E, ascorbic acid), minerals (e.g., calcium, iron, zinc), and bioactive substances (e.g., flavonoids). Through concentrated extraction, its nutrients are highly enriched, with an extraction ratio of 15-40:1. This extract not only provides comprehensive nutritional support for the human body but also possesses antioxidant and anti-inflammatory physiological activities. The antioxidants within it can scavenge free radicals in the body, reduce oxidative stress damage to cells, and help delay aging and prevent chronic diseases.

[0021] Whey protein: Whey protein is a high-quality complete protein containing all eight essential amino acids in a composition ratio close to human needs, making it easily absorbed and utilized by the body. It also contains various bioactive components, such as β-lactoglobulin, α-lactalbumin, and immunoglobulins. These components endow whey protein with multiple health benefits, such as enhancing immunity, promoting muscle growth and repair, and regulating gut microbiota. In the nutritional food of this invention, whey protein can supplement the protein needed by the human body and maintain normal physiological functions.

[0022] Phycocyanin: Phycocyanin possesses a variety of important physiological functions. It can regulate the human immune system, enhance immune function, and improve the body's resistance to disease. Studies have shown that phycocyanin can stimulate the activity of immune cells and promote the secretion of immune factors, thereby enhancing the body's immune response. Furthermore, phycocyanin also has antioxidant and free radical scavenging effects, protecting cells from free radical damage and promoting healthy cell growth. Simultaneously, it also has certain effects on inhibiting cancer cell growth, promoting human cell regeneration, and maintaining ovarian health.

[0023] Collagen peptides: Collagen peptides have a molecular weight between 200-1000 Daltons, making them easier for the body to absorb compared to large collagen molecules. Collagen peptides can replenish collagen in tissues such as skin and joints, enhancing skin elasticity, reducing wrinkles, and also having a positive impact on joint health. They can promote the repair and regeneration of articular cartilage, relieve joint pain, and improve joint flexibility.

[0024] Glucan: Glucan is a polysaccharide with immunomodulatory functions. It can enhance the body's immunity by activating immune cells, promoting their proliferation and activity, and improving the body's resistance. In addition, glucan can regulate the balance of intestinal flora, promoting the growth of beneficial bacteria, inhibiting the proliferation of harmful bacteria, and maintaining a healthy intestinal environment. In food, glucan also has thickening and stabilizing effects, improving the texture and taste of food.

[0025] The second embodiment: This invention also discloses a method for preparing a nutritional food containing small molecule active peptides, mainly including three key steps: preparation of deer blood small molecule active peptides, preparation of concentrated extract of *Gnaphalium affine*, and mixed preparation. Preparation of small molecule bioactive peptides from deer blood: First, fresh deer blood is dried and ground into deer blood powder. The deer blood powder is mixed with distilled water at a weight ratio of 1:8 to 1:20, and sonicated at 120-180W for 25-50 minutes. Then, it is kept at a constant temperature and shaken for 70-110 minutes to obtain a deer blood solution. Ultrasonic treatment can disrupt the cell structure in deer blood, promote protein dissolution, and improve the efficiency of subsequent enzymatic hydrolysis. Constant temperature shaking helps to thoroughly mix the deer blood powder and distilled water, creating favorable conditions for the enzymatic hydrolysis reaction.

[0026] Acidic protease was added to the deer blood solution, and the pH was adjusted to 2.2-2.8 with 1.0 mol / L HCl. The enzymatic hydrolysis reaction was carried out in a constant-temperature shaking water bath at 37-62℃ for 1.5-7.0 h. During the hydrolysis process, acidic reagent was continuously added to maintain pH stability. The acidic protease specifically cleaves specific peptide bonds in deer blood proteins, breaking down large protein molecules into smaller peptide fragments.

[0027] After enzymatic hydrolysis, the pH of the deer blood solution was adjusted to 7.2-7.8 with 1.0 mol / L NaOH. Then, neutral protease was added, and enzymatic hydrolysis continued for 1.5-7.0 hours in a constant-temperature shaking water bath at 37-62℃, while an alkaline reagent was added to maintain pH stability. Neutral protease further hydrolyzes the peptides degraded by acidic protease, ensuring the deer blood protein is fully hydrolyzed into small-molecule bioactive peptides. The mass of acidic protease was 0.4%-0.8% of the deer blood powder, and the mass ratio of neutral protease to acidic protease was 1-4:1. This enzyme dosage and ratio ensured efficient enzymatic hydrolysis, yielding deer blood small-molecule bioactive peptides with good biological activity.

[0028] After enzymatic hydrolysis, the hydrolysate is inactivated by high-temperature stirring at 92-98℃ for 8-25 minutes to terminate the enzymatic hydrolysis reaction. After cooling to room temperature, it is centrifuged at 8200-9300 rpm for 12-25 minutes, and the supernatant is extracted to obtain the deer blood small molecule active peptide enzymatic hydrolysate. High-temperature inactivation of the enzyme can deactivate the protease, avoiding adverse effects of the enzyme on product quality. Centrifugation can remove impurities from the hydrolysate and improve the purity of the deer blood small molecule active peptides.

[0029] The enzymatic hydrolysate of deer blood small molecule active peptides was sequentially added to β-cyclodextrin and yeast for deodorization. The mass ratio of β-cyclodextrin to deer blood small molecule active peptides was 2.5%-3.5%, and deodorization was carried out at 32-55℃ for 40-100 min; the mass ratio of yeast to deer blood small molecule active peptides was 0.8%-1.5%, and deodorization was carried out at 32-45℃ for 40-100 min. β-cyclodextrin can remove the fishy smell of deer blood through molecular inclusion, while yeast can further improve the odor of deer blood small molecule active peptides through its own metabolism. The supernatant was collected by centrifugation, pre-frozen for 15-40 h, and then freeze-dried to obtain freeze-dried powder of deer blood small molecule active peptides. Freeze-drying technology can remove water by sublimation at low temperature, maximizing the preservation of the bioactivity of deer blood small molecule active peptides.

[0030] Preparation of concentrated extract from *Hedyotis diffusa*: Fresh *Hedyotis diffusa* is washed to remove surface impurities and contaminants. Low-temperature drying technology is used, drying at 40-60℃ until the moisture content is below 8%. Low-temperature drying avoids the loss of nutrients in the *Hedyotis diffusa* due to high temperatures. The dried *Hedyotis diffusa* is then pulverized and passed through a 60-100 mesh sieve to make the particles more uniform, facilitating subsequent extraction operations.

[0031] The pulverized edible grass is placed in an extraction device and extracted 2-4 times at 50-80℃ using water or ethanol as solvent, with each extraction lasting 1-3 hours. Multiple extractions ensure the full extraction of nutrients from the edible grass. The extracts are combined, filtered to remove impurities, and then concentrated under reduced pressure and further concentrated to obtain a fluid extract. The fluid extract is then spray-dried, pulverized, and passed through an 80-120 mesh sieve to obtain a concentrated edible grass extract. Spray drying rapidly converts the fluid extract into a dry, powdered extract, facilitating storage and use.

[0032] Mixing Preparation: Deer blood small molecule active peptide freeze-dried powder, concentrated extract of *Gnaphalium affine*, whey protein, phycocyanin, collagen peptides, and dextran are mixed according to the above weight proportions. Depending on product requirements, appropriate amounts of sweeteners (such as sucrose, xylitol, etc.), acidulants (such as citric acid, malic acid, etc.), and stabilizers such as pectin and sodium carboxymethyl cellulose can be added. Add an appropriate amount of purified water, stir well, and prepare the corresponding nutritional food dosage form. If prepared as an oral liquid, filter with a 0.22μm filter membrane to remove any possible impurities, then fill and sterilize to ensure product quality and safety. If prepared as a solid beverage, perform subsequent processing such as drying, granulation, and packaging for convenient storage and use by consumers.

[0033] The third implementation method includes three examples. Example 1: Oral liquid dosage form Raw material preparation Accurately weigh out the following components according to weight: 20 parts deer blood small molecule active peptides, 15 parts edible leaf grass concentrated extract, 25 parts whey protein, 10 parts phycocyanin, 8 parts collagen peptides, and 5 parts glucan.

[0034] Prepare appropriate amounts of sucrose and citric acid as flavoring agents, pectin as a stabilizer, and sufficient purified water.

[0035] Preparation of small molecule active peptides from deer blood Fresh deer blood was collected, dried, and ground into deer blood powder. The deer blood powder was mixed with distilled water at a weight ratio of 1:12, and placed in an ultrasonic instrument. The ultrasonic power was set to 150W, and the mixture was ultrasonicated for 35 minutes. The mixture was then transferred to a constant temperature shaker and shaken at 37°C for 90 minutes to obtain a deer blood solution.

[0036] Add acidic protease to the deer blood solution, the mass of which is 0.6% of the deer blood powder. Adjust the pH of the solution to 2.5 with 1.0 mol / L HCl, and place it in a 37℃ constant temperature shaking water bath for 4 hours for enzymatic hydrolysis, during which acidic reagent is continuously added to maintain pH stability.

[0037] After enzymatic hydrolysis, the pH was adjusted to 7.5 with 1.0 mol / L NaOH, and neutral protease was added at a mass ratio of 2:1 to acidic protease. Enzymatic hydrolysis was continued in a 37°C constant temperature shaking water bath for 4 hours, while alkaline reagent was added dropwise to maintain the pH.

[0038] After enzymatic hydrolysis, transfer the hydrolysate to a magnetic stirrer and inactivate the enzyme at 95°C for 15 minutes. After cooling to room temperature, place it in a centrifuge and centrifuge at 8500 rpm for 20 minutes, then collect the supernatant.

[0039] β-Cyclodextrin and yeast were added sequentially to the supernatant for deodorization. The mass ratio of β-cyclodextrin to deer blood small molecule active peptides was 3%, and deodorization was carried out at 40℃ for 60 min; the mass ratio of yeast to deer blood small molecule active peptides was 1.2%, and deodorization was carried out at 35℃ for 60 min. After deodorization, the supernatant was collected by centrifugation, pre-frozen for 24 h, and then freeze-dried to obtain freeze-dried powder of deer blood small molecule active peptides.

[0040] Preparation of concentrated extract of edible leaves Select fresh edible leaves, wash them thoroughly, and dry them in a 45℃ oven until the moisture content is below 8%. After drying, crush the edible leaves using a grinder and pass them through an 80-mesh sieve.

[0041] The pulverized edible leaves were placed in an extraction tank and extracted three times at 60°C, each time for 2 hours, using water as the solvent. The extracts were combined, impurities were removed by filtration, and then concentrated under reduced pressure and further concentrated to obtain a fluid extract.

[0042] The fluid extract was spray-dried, and the dried powder was passed through a 100-mesh sieve to obtain the concentrated extract of *Hedyotis diffusa*.

[0043] Mixed preparation of oral liquid The deer blood small molecule active peptide freeze-dried powder, phycocyanin concentrated extract, whey protein, phycocyanin, collagen peptides, and dextran were added to the mixing tank in sequence.

[0044] Add appropriate amounts of sucrose and citric acid to the mixing bowl for flavoring, add pectin as a stabilizer, and then add sufficient purified water. Turn on the mixer and stir thoroughly until all ingredients are completely dissolved and mixed evenly.

[0045] The mixture is filtered through a 0.22 μm filter membrane to remove any impurities and particles. The filtered solution is then bottled into glass or plastic bottles and sterilized at high temperature to obtain the finished oral liquid product.

[0046] Example 2: Solid Beverage Dosage Form Raw material preparation Accurately weigh 25 parts of deer blood small molecule active peptides, 20 parts of concentrated extract of edible leaves, 30 parts of whey protein, 15 parts of phycocyanin, 10 parts of collagen peptides, and 8 parts of glucan.

[0047] Prepare xylitol and malic acid as flavoring agents, and sodium carboxymethyl cellulose as a stabilizer.

[0048] Preparation of small molecule active peptides from deer blood Deer blood powder and distilled water were mixed at a weight ratio of 1:15, ultrasonicated at 160W for 40 minutes, and then shaken at 37℃ for 100 minutes to obtain a deer blood solution.

[0049] Add acidic protease (0.7% of deer blood powder by mass), adjust the pH to 2.6 with 1.0 mol / L HCl, and enzymatically hydrolyze for 5 h in a constant temperature shaking water bath at 45℃.

[0050] Adjust the pH to 7.6 with 1.0 mol / L NaOH, add neutral protease (mass ratio of 3:1 to acidic protease), and continue enzymatic hydrolysis for 5 hours in a constant temperature shaking water bath at 45℃.

[0051] Inactivate the enzyme at 96℃ for 20 min, then cool and centrifuge at 9000 rpm for 22 min, and collect the supernatant.

[0052] Add β-cyclodextrin (3.2% by mass of deer blood small molecule active peptides) and deodorize at 45℃ for 80 min, then add yeast (1.3% by mass of deer blood small molecule active peptides) and deodorize at 40℃ for 80 min. After pre-freezing for 30 h, freeze-dry to obtain deer blood small molecule active peptide freeze-dried powder.

[0053] Preparation of concentrated extract of edible leaves Fresh edible leaves are washed and dried at 50℃ until the moisture content is below 8%, then crushed and passed through a 90-mesh sieve.

[0054] The extract was extracted three times at 70°C for 2.5 hours each time using ethanol as the solvent. The extract was filtered and concentrated to obtain a fluid extract, which was then spray-dried and passed through a 110-mesh sieve to obtain a concentrated extract of *Hedyotis diffusa*.

[0055] Mixed preparation of solid beverage Pour deer blood small molecule active peptide freeze-dried powder, phycocyanin concentrated extract, whey protein, phycocyanin, collagen peptides, and dextran into a mixer.

[0056] Add xylitol and malic acid for flavoring, and sodium carboxymethyl cellulose as a stabilizer, and mix thoroughly.

[0057] The mixed materials are dried to control the moisture content within a certain range. The dried materials are then granulated using a granulator and packaged to obtain the finished solid beverage product.

[0058] Example 3: Nutritional Jelly Formulation Raw material preparation Weigh out 18 parts of deer blood small molecule active peptides, 12 parts of concentrated extract of edible leaves, 20 parts of whey protein, 8 parts of phycocyanin, 6 parts of collagen peptides, and 4 parts of glucan.

[0059] Prepare honey and sodium citrate as flavoring agents, and pectin and sodium carboxymethyl cellulose as a combination of stabilizers.

[0060] Preparation of small molecule active peptides from deer blood Deer blood powder and distilled water were mixed at a ratio of 1:10, ultrasonicated at 140W for 30 minutes, and then oscillated at 37℃ for 80 minutes to obtain a deer blood solution.

[0061] Add acidic protease (0.5% of the deer blood powder by mass), adjust the pH to 2.4 with 1.0 mol / L HCl, and enzymatically hydrolyze for 3 hours in a constant temperature shaking water bath at 40℃.

[0062] Adjust the pH to 7.4 with 1.0 mol / L NaOH, add neutral protease (mass ratio of 1.5:1 to acidic protease), and continue enzymatic hydrolysis for 3 hours in a constant temperature shaking water bath at 40℃.

[0063] Inactivate the enzyme at 93℃ for 10 min, then centrifuge at 8300 rpm for 15 min after cooling, and collect the supernatant.

[0064] Add β-cyclodextrin (2.8% by mass of deer blood small molecule active peptides) and deodorize at 38℃ for 50 min, then add yeast (1% by mass of deer blood small molecule active peptides) and deodorize at 33℃ for 50 min. After pre-freezing for 20 h, freeze-dry to obtain deer blood small molecule active peptide freeze-dried powder.

[0065] Preparation of concentrated extract of edible leaves Fresh edible leaves are washed and dried at 40℃ until the moisture content is below 8%, then crushed and passed through a 70-mesh sieve.

[0066] The extract was extracted twice at 55°C for 2 hours each time using water as a solvent. After treatment, a fluid extract was obtained, which was spray-dried and passed through a 90-mesh sieve to obtain a concentrated extract of *Hedyotis diffusa*.

[0067] Mix and prepare nutritional jelly Place deer blood small molecule active peptide freeze-dried powder, phycocyanin concentrated extract, whey protein, phycocyanin, collagen peptides, and dextran into a container.

[0068] Add honey and sodium citrate for flavoring, add a stabilizer made of pectin and sodium carboxymethyl cellulose, and add an appropriate amount of purified water and stir well.

[0069] Heat the mixture to a suitable temperature to fully dissolve and mix it, then pour it into a jelly mold. After cooling and solidification, package it to obtain the finished nutritious jelly product.

[0070] Effect verification: Fifty volunteers with weakened immune systems were selected and divided into two groups. One group took the nutritional supplement described in this embodiment, while the other group took a placebo. After three months of continuous use, the volunteers taking the nutritional supplement showed an average increase of 20% in white blood cell count, a significant increase in immunoglobulin G levels, a marked reduction in fatigue, and improved sleep quality. Analysis of immune indicators in the volunteers' blood revealed enhanced activity of T lymphocytes and B lymphocytes, indicating that the nutritional supplement of this invention can effectively regulate the immune system and enhance the body's immunity.

[0071] This invention is the first to scientifically combine multiple ingredients with unique nutritional functions, such as deer blood small molecule active peptides and concentrated extracts of edible leaves. These ingredients encompass a variety of nutrients, including proteins, small molecule active peptides, vitamins, minerals, and polysaccharides, which work synergistically to simultaneously meet the body's diverse nutritional needs, such as enhancing immunity, providing antioxidant effects, and repairing cells. This complex formula overcomes the limitations of traditional nutritional foods with their single-ingredient composition, providing consumers with a more comprehensive and efficient nutritional supplementation solution.

[0072] In the preparation of deer blood small molecule active peptides, a two-step enzymatic hydrolysis technique using acidic and neutral proteases, combined with precise pH and temperature control, is employed. This method effectively increases the degree of hydrolysis of deer blood peptides, making the resulting small molecule active peptides more easily absorbed by the human body. Simultaneously, the deodorizing process using β-cyclodextrin and yeast cleverly solves the problem of the fishy smell of deer blood, improving the product's taste and acceptability. The concentrated extract of *Hedyotis diffusa* is prepared using low-temperature drying and a multi-step extraction process, maximizing the preservation of its nutrients and avoiding the loss of nutrients in traditional processing methods.

[0073] The nutritional food of this invention not only supplements the nutrients needed by the human body, but also has significant functions such as immune regulation, anti-oxidation, and cell repair. Animal experiments and clinical studies have verified that it can effectively improve the body's immunity, reduce free radical damage to cells, promote cell regeneration and repair, and has preventive and ameliorative effects on various chronic diseases. For example, animal experiments have shown that after consuming the nutritional food of this invention, experimental animals showed a significant increase in immune indicators such as white blood cell count and immune organ index, enhanced antioxidant enzyme activity, and effective repair of cell damage.

[0074] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0075] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0076] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A nutritional food containing small molecule active peptides, characterized in that: By weight, it consists of 15-35 parts deer blood small molecule active peptides, 10-30 parts concentrated extract of edible leaves, 15-40 parts whey protein, 5-20 parts phycocyanin, 3-15 parts collagen peptides, and 2-10 parts glucan.

2. The nutritional food containing small molecule active peptides according to claim 1, characterized in that: The average molecular weight of the deer blood small molecule active peptides is between 100 and 800 Daltons.

3. A nutritional food containing small molecule active peptides according to claim 1, characterized in that: The concentration ratio of the concentrated extract of the herbaceous plant is 15-40:

1.

4. A nutritional food containing small molecule active peptides according to claim 1, characterized in that: The molecular weight of the collagen peptides is between 200 and 1000 Daltons.

5. A method for preparing a nutritional food containing small molecule active peptides, used to prepare the nutritional food containing small molecule active peptides as described in any one of claims 1-4, characterized in that, Includes the following steps: Step 1: Dry and grind fresh deer blood into deer blood powder. Mix deer blood powder with distilled water at a weight ratio of 1:8 to 1:

20. Sonicate at 120-180W for 25-50 minutes and then oscillate at a constant temperature for 70-110 minutes to obtain a deer blood solution. Step 2: Add acidic protease to the deer blood solution, adjust the pH to 2.2-2.8 with 1.0 mol / L HCl, and enzymatically hydrolyze in a constant temperature shaking water bath at 37-62℃ for 1.5-7.0 h, maintaining pH stability during the enzymatic hydrolysis process; Step 3: Adjust the pH to 7.2-7.8 with 1.0 mol / L NaOH, add neutral protease, and enzymatically hydrolyze in a constant temperature shaking water bath at 37-62℃ for 1.5-7.0 h, maintaining pH stability during the enzymatic hydrolysis. After enzymatic hydrolysis, inactivate the enzyme at 92-98℃ using a magnetic stirrer for 8-25 min, cool, and centrifuge at 8200-9300 rpm for 12-25 min to extract the supernatant. Step 4: Add β-cyclodextrin and yeast to the supernatant sequentially for deodorization. The mass ratio of β-cyclodextrin to deer blood small molecule active peptides is 2.5%-3.5%. Deodorize at 32-55℃ for 40-100 min. The mass ratio of yeast to deer blood small molecule active peptides is 0.8%-1.5%. Deodorize at 32-45℃ for 40-100 min. Centrifuge and collect the supernatant. Pre-freeze for 15-40 h and then freeze-dry to obtain deer blood small molecule active peptide freeze-dried powder. Step 5: Wash the edible leaves, dry them at low temperature until the moisture content is less than 8%, pulverize them through a 60-100 mesh sieve, extract them 2-4 times at 50-80℃ with water or ethanol as solvent, each time for 1-3 hours, combine the extracts, filter them, concentrate them under reduced pressure, concentrate them again to obtain a fluid extract, spray dry them, and pulverize them through an 80-120 mesh sieve to obtain the concentrated edible leaves extract. Step Six: Mix deer blood small molecule active peptide freeze-dried powder, edible leaf grass concentrated extract, whey protein, phycocyanin, collagen peptide, and dextran in proportion. Flavoring agents and stabilizers may be added. Add an appropriate amount of purified water and stir evenly to prepare the corresponding dosage form.

6. The method for preparing a nutritional food containing small molecule active peptides according to claim 5, characterized in that: The mass of the acidic protease is 0.4%-0.8% of the deer blood powder, and the mass ratio of the neutral protease to the acidic protease is 1-4:

1.

7. The method for preparing a nutritional food containing small molecule active peptides according to claim 5, characterized in that: The leafy grass is dried at a low temperature of 40-60℃.

8. The method for preparing a nutritional food containing small molecule active peptides according to claim 5, characterized in that: When the prepared formulation is an oral liquid, it is filtered through a 0.22μm filter membrane, filled, and sterilized; when the prepared formulation is a solid beverage, it is dried, granulated, and packaged.

9. The method for preparing a nutritional food containing small molecule active peptides according to claim 5, characterized in that: The flavoring agent is one or more of sweeteners and acidifiers, and the stabilizer is one or more of pectin and sodium carboxymethyl cellulose.

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