Oily skin care composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetic functional raw materials, in particular to an oily skin care composition as well as a preparation method and application thereof. On one hand, the oily skin care composition provided by the invention is prepared from the following components in parts by mass: 95 to 99.75 parts of radix puerariae extract and 0.25 to 5 parts of carnosine. The radix puerariae extract and carnosine in a specific proportion are combined, so that the anti-squalene-oxidation skin care composition has an excellent anti-squalene-oxidation effect, an excellent oil control effect can be further realized, and the nursing effect on oily skin is remarkably enhanced. The preparation method of the oily skin care composition is simple in process and suitable for industrial production, and has wide application prospects in cosmetics and / or pharmaceuticals with oil control requirements.

Description

Technical Field

[0001] This invention relates to the field of cosmetic efficacy ingredients, and in particular to an oily skin care composition, its preparation method, and its application. Background Technology

[0002] Abnormal skin lipid metabolism, particularly the combined effects of excessive secretion and squalene oxidation, is a key pathological basis for the development of oily skin and acne. Squalene, a naturally occurring nonpolar lipid in sebum, accounts for approximately 10%–14% of total epidermal lipids. Its chemical structure contains six double bonds, making it highly susceptible to attack from ultraviolet radiation, environmental pollutants, and reactive oxygen species (ROS) produced by skin surface microorganisms (such as Propionibacterium acnes). This leads to lipid peroxidation, generating squalene monohydroperoxide (SQOOH) and other oxidation products. Studies have shown that oxidized squalene has significant inflammatory and comedogenic properties, inducing keratinocytes to produce inflammatory factors such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), and promoting abnormal keratinization of the hair follicle opening, thus clogging pores and inducing or exacerbating acne lesions.

[0003] Current oil-control skincare technologies mostly focus on physical oil absorption, inhibiting sebum synthesis, or regulating sebaceous gland activity. For example, they use ingredients such as zinc salts, niacinamide, and salicylic acid to reduce sebum secretion or accelerate exfoliation. However, these methods generally overlook the secondary damage caused by the subsequent oxidation of squalene already secreted to the skin surface. Even if the total amount of sebum is controlled, residual squalene can still continue to oxidize under external stimuli, leading to increased oxidative stress levels and a chronic low-grade inflammatory state.

[0004] In view of this, the present invention is hereby proposed. Summary of the Invention

[0005] One object of the present invention is to provide an oily skin care composition with excellent anti-squalene oxidation effect, which can further achieve excellent oil control effect.

[0006] Another object of the present invention is to provide a method for preparing the above-mentioned oily skin care composition, which is simple in process and suitable for industrial production.

[0007] A third objective of this invention is to provide an application of the above-mentioned oily skin care composition in the preparation of cosmetics and / or pharmaceuticals, which has excellent anti-squalene oxidation effect and has broad application prospects in cosmetics and / or pharmaceuticals with oil control requirements.

[0008] To achieve the above-mentioned objectives of the present invention, one aspect of the present invention provides an oily skin care composition, comprising, by weight parts, the following components:

[0009] 95-99.75 parts of kudzu root extract and 0.25-5 parts of carnosine.

[0010] The oily skin care composition includes a specific ratio of kudzu root extract and carnosine, which has excellent anti-squalene oxidation effect and can further achieve excellent oil control effect, significantly enhancing the care effect for oily skin.

[0011] Another aspect of the present invention provides a method for preparing an oily skin care composition, comprising: mixing kudzu root extract with carnosine evenly. This method is simple and suitable for industrial production.

[0012] Another aspect of the present invention provides the use of the above-described oily skin care composition in the preparation of cosmetics and / or pharmaceuticals.

[0013] The above-mentioned oily skin care composition has excellent anti-squalene oxidation effect and has broad application prospects in cosmetics and / or pharmaceuticals with oil control requirements.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0015] This invention utilizes a specific ratio of kudzu root extract and carnosine, exhibiting excellent anti-squalene oxidation effects and further enhancing oil control, thus significantly improving the skincare benefits for oily skin. The preparation method of the above composition is simple, suitable for industrial production, and has broad application prospects in cosmetics and / or pharmaceuticals requiring oil control. Attached Figure Description

[0016] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0017] Figure 1 The figure shows the test results for resistance to squalene oxidation in a specific embodiment of the present invention. Detailed Implementation

[0018] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings and specific embodiments. However, those skilled in the art will understand that the embodiments described below are some embodiments of the present invention, but not all embodiments, and are only used to illustrate the present invention, and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0019] In one aspect, the present invention provides an oily skin care composition, comprising, by weight, the following components:

[0020] 95-99.75 parts of kudzu root extract and 0.25-5 parts of carnosine.

[0021] The oily skin care composition includes a specific ratio of kudzu root extract and carnosine, which has excellent anti-squalene oxidation effect and can further achieve excellent oil control effect, significantly enhancing the care effect for oily skin.

[0022] The kudzu root extract can be commercially available kudzu root extract and can be prepared using various processes including water extraction and alcohol extraction. Preferably, it is a kudzu root extract rich in flavonoids. More preferably, it can be prepared using the method described in patent ZL202310806931.X.

[0023] In one specific embodiment of the present invention, the method for preparing the kudzu root extract includes:

[0024] The first mixture of kudzu root, solvent, and activated carbon is subjected to thermal reflux extraction, followed by a first solid-liquid separation and collection of the first liquid phase. The pH of the first liquid phase is adjusted using an alkaline substance to obtain a second mixture. The second mixture is then mixed with a first material, which is selected from at least one of glycyrrhizic acid, glycyrrhetinic acid, and glycyrrhizate, and subjected to a second solid-liquid separation to collect the second liquid phase.

[0025] This invention employs a combination of solvent and activated carbon to treat kudzu root. Activated carbon is a loose, porous carbon particle with numerous capillary structures. This structure gives activated carbon a large specific surface area, enabling it to fully contact substances and form intermolecular forces to adsorb impurities in liquids and gases. Due to the complex composition of kudzu root, its extracts are usually dark in color. This invention uses a mixed system of solvent and activated carbon to simultaneously extract and decolorize kudzu root. The two work synergistically to ensure maximum improvement in the extract color and adsorption of impurities in kudzu root, effectively dissolving flavonoids.

[0026] In one specific embodiment of the present invention, the glycyrrhizic acid salt includes at least one of ammonium glycyrrhizate, sodium glycyrrhizate, monopotassium glycyrrhizate, and dipotassium glycyrrhizate.

[0027] The first material of the present invention has a good solubilizing effect on flavonoid components and will cause certain components in the flavonoid extract to precipitate under heating conditions.

[0028] Ammonium glycyrrhizate possesses an amphiphilic structure and surfactant-like effects. Its general increase in the solubility of monomeric components in traditional Chinese medicine is largely related to the hydrophobic cavities it forms in aqueous solutions. Ammonium glycyrrhizate exhibits a strong solubilizing effect on flavonoids. Since many flavonoids contain phenolic hydroxyl structures, the solubilizing and stabilizing effect of ammonium glycyrrhizate on flavonoids may also be related to weak intermolecular interactions such as hydrogen bonds. Ammonium glycyrrhizate can self-assemble into dimers in aqueous solutions, and under certain conditions, aggregate to form micelles or gels with specific spatial structures. The hydrophobic cavities formed by the self-assembly of ammonium glycyrrhizate in aqueous solutions can encapsulate flavonoid compounds, effectively increasing their solubility and preventing precipitation. Furthermore, the addition of ammonium glycyrrhizate can cause certain components in flavonoid extracts to precipitate under heating conditions; these impurities can then be removed from the extract through solid-liquid separation.

[0029] Since both flavonoids and ammonium glycyrrhizate are weakly acidic, directly adding ammonium glycyrrhizate to the extract will lower the pH of the extract, causing the weakly acidic flavonoids to precipitate. This affects the content of the effective components in the extract. Therefore, this invention uses an alkaline substance to adjust the pH of the first liquid phase system to weakly acidic, neutral, or weakly alkaline before adding ammonium glycyrrhizate, avoiding the precipitation of flavonoids and facilitating the formation of a self-assembled system with ammonium glycyrrhizate and flavonoids. In one embodiment, the pH of the first liquid phase system is adjusted to 6.5–8 using an alkaline substance, such as 7, 7.2, 7.3, 7.5, 7.7, 7.8, 7.9, or 8. A pH that is too high will affect the color of the flavonoids and impact the decolorization effect. In one embodiment, the alkaline substance includes sodium hydroxide solution.

[0030] The method of the present invention can improve the extraction efficiency of flavonoids in kudzu root extract, promote their targeted enrichment, increase the relative content of flavonoids in the targeted enrichment, and ensure the effect.

[0031] In one specific embodiment of the present invention, the kudzu root is pre-crushed and sieved.

[0032] In one specific embodiment of the present invention, the pulverization process uses coarse powder with a particle size of 0.1 to 0.3 cm.

[0033] In one specific embodiment of the present invention, the sieving process uses a 10-mesh sieve.

[0034] This invention pre-processes kudzu root by crushing and sieving to obtain kudzu root of suitable particle size, which is more conducive to subsequent extraction.

[0035] In one specific embodiment of the present invention, the mass ratio of kudzu root, solvent, activated carbon and the first material is 100:(1000~2000):(3.2~9.6):(8~64).

[0036] In one specific embodiment of the present invention, the solvent includes at least one of water, monohydric alcohol, and polyhydric alcohol.

[0037] In one specific embodiment of the present invention, the monohydric alcohol includes at least one selected from methanol, ethanol, 1-propanol, 2-propanol, n-butanol, isobutanol, 1-pentanol, 2-pentanol, 3-pentanol, 1-hexanol, 2-hexanol, 3-hexanol, 1-heptanol, 2-heptanol, 3-heptanol, 4-heptanol, 1-octanol, 2-octanol, 3-octanol, 4-octanol, 1-nonanol, 2-nonanol, 3-nonanol, 4-nonanol, 5-nonanol, 1-decanol, 2-decanol, 3-decanol, 4-decanol, and 5-decanol.

[0038] In one specific embodiment of the present invention, the polyol includes at least one selected from ethylene glycol, 1,2-propanediol, 1,3-propanediol, glycerol, 1,2-butanediol, 1,3-butanediol, 1,4-butanediol, 1,2,3-butanetriol, 1,2,4-butanetriol, 1,2-pentanediol, 1,3-pentanediol, 1,4-pentanediol, 1,5-pentanediol, 2,3-pentanediol, 2,4-pentanediol, 1,2,5-pentanetriol, and 1,2-hexanediol.

[0039] In one specific embodiment of the present invention, the solvent is at least one of a monohydric alcohol and a polyhydric alcohol and water, wherein the mass fraction of the monohydric alcohol and / or the polyhydric alcohol is M, 0.1% ≤ M < 100%, for example 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, etc.

[0040] In one specific embodiment of the present invention, the mass ratio of kudzu root, solvent, activated carbon and the first material is 100:1000:3.2:8, 100:1000:4:10, 100:1500:5:15, 100:2000:9.6:30, etc.

[0041] In one specific embodiment of the present invention, by mass parts, the kudzu root comprises 100 parts, the activated carbon comprises 3.2 to 9.6 parts, and the first material comprises 8 to 64 parts, with the mass ratio of kudzu root to solvent being 1:(10 to 20). The present invention uses an appropriate mass ratio of kudzu root, solvent, and activated carbon, which is more conducive to ensuring the extraction, decolorization, and removal of impurities of flavonoids, improving extraction efficiency, and increasing the targeted enrichment. The present invention uses an appropriate amount of the first material, which is more conducive to increasing the relative content of flavonoids in the final targeted enrichment and reducing the impurity content.

[0042] In one specific embodiment of the present invention, the temperature of the hot reflux extraction is 75-85°C, for example 75°C, 76°C, 77°C, 78°C, 79°C, 80°C, 81°C, 82°C, 85°C, etc.; the time of the hot reflux extraction is 110-130 min, for example 110 min, 115 min, 120 min, 125 min, 130 min, etc.

[0043] In one specific embodiment of the present invention, the first solid-liquid separation includes coarse filtration and fine filtration performed sequentially.

[0044] In one specific embodiment of the present invention, the coarse filter uses 200-mesh gauze, YB06 and F100 filter plates.

[0045] In one specific embodiment of the present invention, the coarse filtration sequentially employs 200-mesh gauze, YB06 and F100 filter plates, that is, the extract is first filtered using 200-mesh gauze, the filtrate is then vacuum filtered using YB06 filter paper, and the filtrate is then vacuum filtered again using F100 filter paper.

[0046] In one specific embodiment of the present invention, the fine filter uses H70 paperboard. The present invention effectively separates and removes impurities through coarse and fine filtration.

[0047] In one specific embodiment of the present invention, the heat treatment temperature is 85–90°C, for example, 85°C, 86°C, 87°C, 88°C, 89°C, or 90°C. By using a suitable heat treatment temperature, the precipitation of impurities can be effectively promoted, the purity of flavonoids in the directional enrichment can be improved, and its performance can be guaranteed.

[0048] In one specific embodiment of the present invention, the second solid-liquid separation uses H70 filter paper. Through the second solid-liquid separation, impurities are further removed, improving the purity of the product.

[0049] In one specific embodiment of the present invention, the turbidity of the second liquid phase system is less than 2 FNU.

[0050] In one specific embodiment of the present invention, the method further includes: heating and sterilizing the second liquid phase system to remove contaminants.

[0051] In one specific embodiment of the present invention, the temperature for heat sterilization is 80-85°C, such as 81°C, 82°C, 83°C, 84°C, etc., and the heat sterilization time is 30-45 minutes, such as 35 minutes, 40 minutes, 42 minutes, etc.

[0052] Another aspect of the present invention provides a method for preparing an oily skin care composition, comprising: mixing kudzu root extract with carnosine evenly. This method is simple and suitable for industrial production.

[0053] In one specific embodiment of the present invention, kudzu root extract and carnosine are mixed evenly and then sterilized.

[0054] In one specific embodiment of the present invention, the sterilization temperature is 75-79°C, such as 75°C, 76°C, 77°C, 78°C, 79°C, etc., and the sterilization time is 20-40 minutes, such as 20 minutes, 30 minutes, 40 minutes, etc.

[0055] Another aspect of the present invention provides the use of the above-described oily skin care composition in the preparation of cosmetics and / or pharmaceuticals.

[0056] The above-mentioned oily skin care composition has excellent anti-squalene oxidation effect and has broad application prospects in cosmetics and / or pharmaceuticals with oil control requirements.

[0057] Example 1

[0058] Preparation of an oily skin care composition, comprising:

[0059] Pueraria lobata extract (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Ge Qingyan) and carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) were mixed evenly, with the mass ratio of pueraria lobata extract to carnosine being 97.5:2.5. The mixture was then sterilized at 77°C for 30 minutes.

[0060] Example 2

[0061] Preparation of an oily skin care composition, comprising:

[0062] Pueraria lobata extract (prepared using the method in Example 10 of patent ZL202310806931.X) and carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) were mixed evenly, with the mass ratio of pueraria lobata extract to carnosine being 95:5%. The mixture was sterilized at 79°C for 20 minutes.

[0063] Example 3

[0064] The specific steps for preparing an oily skin care composition are as follows:

[0065] Pueraria lobata extract (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Ge Qingyan) and carnosine (purchased from Changzhou Kaixing Biotechnology Co., Ltd., CAS No. 305-84-0) were mixed evenly, with the mass ratio of pueraria lobata extract to carnosine being 99.75:0.25. The mixture was sterilized at 75℃ for 40 min.

[0066] Comparative Example 1

[0067] Kudzu root extract (provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd., trade name Ge Qingyan).

[0068] Comparative Example 2

[0069] Carnosine (purchased from Changzhou Kaixin Biotechnology Co., Ltd., CAS No. 305-84-0).

[0070] Experimental Example

[0071] The anti-squalene oxidation effect of the oily skin care composition of the present invention was evaluated.

[0072] Squalene was uniformly added to 6-well plates at 600 μL / well. A blank control group, a negative control group, a positive control group (vitamin C), and a sample group were established, with three replicates per group. The sample group was filled with the compositions obtained in Example 1, Comparative Example 1, and Comparative Example 2, respectively. The blank and negative control groups were filled with solvent (ultrapure water), and the positive control group was filled with vitamin C. Vitamin C, the compositions obtained in Example 1, Comparative Example 1, and Comparative Example 2 were dissolved in ultrapure water to achieve concentrations of 1.25 mg / mL, 5% (m / m), 5% (m / m), and 5% (m / m), respectively. 400 μL of each prepared sample solution was added to the corresponding well containing 600 μL of squalene, resulting in sample concentrations of 0.5 mg / mL, 2% (m / m), 2% (m / m), and 2% (m / m), respectively. Except for the blank control group, all other groups were irradiated with UVA at a dose of 360 J / cm². 2 The irradiation time was 6 hours. After the irradiation was completed, the test was immediately performed according to the instructions of the lipid peroxide (LPO) detection kit.

[0073] The relevant conditions are shown in Table 1.

[0074] Table 1 Test conditions for resistance to squalene oxidation

[0075]

[0076] Inhibition rate is calculated as follows: "Inhibition rate (%) = (LPO) 阴性对照组 -LPO 样品组 ) / LPO 阴性对照组 *100% is used for calculation (LPO content (μmol / L) = (A 测定 -A 空白 ) / (A 标准 -A 空白 )*C 标准液 ).

[0077] The results are attached. Figure 1 As shown, from Figure 1 As can be seen, Example 1 exhibits the best anti-squalene oxidation effect. In fact, the anti-squalene oxidation effect of kudzu root extract is generally average (Comparative Example 1), while carnosine has a better anti-squalene oxidation effect (Comparative Example 2). Taking Example 1 as an example, the amount of carnosine used is only 2.5% of the total mass of the composition. If we calculate based on the composition's mass fraction in the sample as 2%, the actual amount of carnosine in the Example 1 sample is only 0.05% of the sample mass, far lower than the generally accepted effective concentration of 1%. This invention combines a very small amount of carnosine with kudzu root extract, which itself has a poor anti-squalene oxidation effect, to achieve a superior anti-squalene oxidation effect that would normally require a large amount of carnosine. The Example 1 sample of this invention contains only 0.05% carnosine by mass, and its anti-squalene oxidation effect is even higher than that of the Comparative Example 2 sample using 2% carnosine by mass. In the oily skin care composition of this invention, kudzu root extract and carnosine exhibit excellent synergistic effects, while avoiding skin sensitivity problems caused by excessive use of carnosine.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An oily skin care composition, characterized in that, Based on parts by mass, it includes the following components: 95-99.75 parts of kudzu root extract and 0.25-5 parts of carnosine.

2. The oily skin care composition according to claim 1, characterized in that, The preparation method of the kudzu root extract includes: The first mixture of kudzu root, solvent, and activated carbon is subjected to thermal reflux extraction, followed by a first solid-liquid separation and collection of the first liquid phase. The pH of the first liquid phase is adjusted using an alkaline substance to obtain a second mixture. The second mixture is then mixed with a first material, which is selected from at least one of glycyrrhizic acid, glycyrrhetinic acid, and glycyrrhizate, and subjected to a second solid-liquid separation to collect the second liquid phase.

3. The oily skin care composition according to claim 2, characterized in that, The kudzu root was pre-crushed; Preferably, the pulverization process uses coarse powder with a particle size of 0.1–0.3 cm; Preferably, the mass ratio of kudzu root, solvent, activated carbon and the first material is 100:(1000~2000):(3.2~9.6):(8~64); Preferably, the glycyrrhizic acid salt includes at least one of ammonium glycyrrhizate, sodium glycyrrhizate, monopotassium glycyrrhizate, and dipotassium glycyrrhizate; Preferably, the solvent includes at least one selected from water, a monohydric alcohol, and a polyhydric alcohol; Preferably, the monohydric alcohol comprises at least one selected from methanol, ethanol, 1-propanol, 2-propanol, n-butanol, isobutanol, 1-pentanol, 2-pentanol, 3-pentanol, 1-hexanol, 2-hexanol, 3-hexanol, 1-heptanol, 2-heptanol, 3-heptanol, 4-heptanol, 1-octanol, 2-octanol, 3-octanol, 4-octanol, 1-nonanol, 2-nonanol, 3-nonanol, 4-nonanol, 5-nonanol, 1-decanol, 2-decanol, 3-decanol, 4-decanol, and 5-decanol. Preferably, the polyol comprises at least one selected from ethylene glycol, 1,2-propanediol, 1,3-propanediol, glycerol, 1,2-butanediol, 1,3-butanediol, 1,4-butanediol, 1,2,3-butanetriol, 1,2,4-butanetriol, 1,2-pentanediol, 1,3-pentanediol, 1,4-pentanediol, 1,5-pentanediol, 2,3-pentanediol, 2,4-pentanediol, 1,2,5-pentanetriol, and 1,2-hexanediol. Preferably, the solvent is at least one of a monohydric alcohol and a polyhydric alcohol and water, wherein the mass fraction of the monohydric alcohol and / or the polyhydric alcohol is M, and 0.1% ≤ M < 100%; Preferably, the temperature of the hot reflux extraction is 75–85°C; and the time of the hot reflux extraction is 110–130 min. Preferably, the first solid-liquid separation includes coarse filtration and fine filtration performed sequentially; Preferably, the coarse filter uses 200-mesh gauze, YB06 and F100 filter plates; Preferably, the fine filter is made of H70 paperboard; Preferably, the pH of the first liquid phase system is adjusted to 6.5-8 using an alkaline substance; Preferably, the temperature of the heat treatment is 85–90°C; Preferably, the second solid-liquid separation uses H70 filter paper. Preferably, the turbidity of the second liquid phase system is less than 2 FNU; Preferably, the method further includes: sterilizing the second liquid phase system by heating; Preferably, the temperature for heat sterilization is 80-85°C, and the heat sterilization time is 30-45 minutes.

4. A method for preparing an oily skin care composition according to any one of claims 1-3, characterized in that, include: Mix kudzu root extract with carnosine until homogeneous.

5. The method for preparing an oily skin care composition according to claim 4, characterized in that, The kudzu root extract and carnosine were mixed evenly and then sterilized. Preferably, the sterilization temperature is 75–79°C, and the sterilization time is 20–40 min.

6. The use of an oily skin care composition as described in any one of claims 1-3 or an oily skin care composition prepared by the method of claim 4 or 5 in the preparation of cosmetics and / or pharmaceuticals.

Images