Ginseng extract processing method suitable for industrial production and capable of selectively increasing content of Rg3 and Rg5

Abstract

The invention relates to a ginseng extract processing method suitable for industrial production and capable of selectively increasing the content of Rg3 and Rg5, and the method comprises the following steps: step 1, taking a ginseng extract, and diluting the ginseng extract by selecting purified water or 70% alcohol water or 30% alcohol water according to the required ratio of Rg3 to Rg5 to prepare a ginseng extract diluted solution; step 2, adding the ginseng extract diluted solution into a sealed glass bottle with a cover, adding freshly squeezed lemon juice, putting into a magnetic stirring rotor, fixing the glass bottle into a constant-temperature water bath kettle, and reacting while stirring; and step 3, after the reaction is finished, extracting with water-saturated n-butyl alcohol, concentrating and drying to obtain a fixed-proportion product rich in ginsenoside Rg3 and Rg5. According to the method, the content of a certain saponin can be selectively increased while the content of ginsenoside Rg3 and ginsenoside Rg5 is increased, so that the ratio of ginsenoside Rg3 to ginsenoside Rg5 is controlled within a reasonable range, customized products can be provided for different people, and the curative effect of the product is improved.

Description

Technical Field

[0001] This invention belongs to the field of natural product processing and transformation technology, specifically relating to a ginseng extract processing method suitable for industrial production that can selectively increase the content of Rg3 and Rg5. Background Technology

[0002] Ginsenosides Rg3 and Rg5 are important active secondary saponins in ginseng, possessing various pharmacological effects such as anti-tumor activity, immune enhancement, and antioxidant activity. However, the content of these two saponins in natural ginseng is extremely low. Currently, industrially, they are mainly prepared by acid-catalyzed hydrolysis of total ginsenosides or protopanaxadiol group saponins. However, existing methods generally suffer from problems such as low selectivity of target products, difficulty in controlling the Rg3 to Rg5 ratio, harsh reaction conditions (potentially involving strong acids and high temperatures), or the need for complex post-processing. These methods also have high equipment requirements or pose safety hazards, hindering industrial application. For example, Chinese patent CN 110302225 A discloses a method for processing red ginseng to increase the content of ginsenosides Rg3, Rg5 and RK1, including the following steps: washing the ginseng and soaking it in a mixture of apple pear juice, lemon juice and water, with a pH of 3.0 to 5.5, for 2 to 3 hours; draining the surface water of the ginseng; steaming at a temperature of 105℃ to 140℃ for 3 to 5 hours; removing the steamed ginseng and drying it at a temperature of 45℃ to 65℃ for 8 to 24 hours, thus obtaining red ginseng rich in ginsenosides Rg3, Rg5 and RK1. Although the ginsenosides Rg3, Rg5, and RK1 content of red ginseng obtained by this method is 20-50 times, 20-30 times, and 75-86 times higher than that of ordinary red ginseng, respectively, it uses red ginseng as raw material, involves a large amount of juice in the processing, has a long processing cycle, and high preparation costs, making it unsuitable for industrial production. Furthermore, the strong acid and high temperature reaction conditions easily lead to side reactions, and the safety of the product cannot be effectively guaranteed. In addition, this method cannot reasonably control the ratio of Rg3 to Rg5, resulting in ambiguous product efficacy and applicability only to a small group of people. Therefore, it is necessary to develop a green, efficient, selective method to increase the content of Rg3 and Rg5, and a mild processing method suitable for industrial scale-up. This is of great significance for the development of functional foods, health products, and pharmaceuticals. Summary of the Invention

[0003] In view of the shortcomings and deficiencies of the existing technology, the purpose of this invention is to provide a ginseng extract processing method suitable for industrial production that can selectively increase the content of Rg3 and Rg5. This method is simple to operate, has mild conditions, and can efficiently and selectively increase the content of ginsenosides Rg3 and Rg5.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: A method for processing ginseng extract suitable for industrial production and selectively increasing the content of Rg3 and Rg5, the method comprising the following steps: Step 1. Take ginseng extract and dilute it with purified water, 70% alcohol water, or 30% alcohol water according to the required ratio of Rg3 and Rg5 to prepare a diluted ginseng extract solution. Step 2. Add the diluted ginseng extract solution to a sealed glass bottle with a lid, add freshly squeezed lemon juice, put in a magnetic stirrer, fix the glass bottle in a constant temperature water bath, and stir to react. Step 3. After the reaction is complete, the product is extracted with water-saturated n-butanol, concentrated and dried to obtain a product rich in ginsenosides Rg3 and Rg5 in a fixed ratio.

[0005] As a preferred embodiment of the present invention, 1g of ginseng extract is taken, and 25ml of purified water, 70% alcohol water, or 30% alcohol water is added to dilute the ginseng extract according to the required ratio of Rg3 and Rg5 to prepare a diluted ginseng extract solution.

[0006] As a preferred embodiment of the present invention, if purified water is used for dilution, 100 μl of freshly squeezed lemon juice is added to 1 ml of ginseng extract dilution solution in step 2, the reaction temperature is controlled at 85℃-95℃, the reaction time is controlled at 3-4 hours, and the content of ginsenoside Rg3 is higher than that of ginsenoside Rg5 by ≥2 times.

[0007] As a preferred embodiment of the present invention, if 70% alcohol-water dilution is used, then in step 2, 200-300 μl of freshly squeezed lemon juice is added to 1 ml of ginseng extract dilution solution, the reaction temperature is controlled at 75°C, and the reaction time is controlled at 5-6 hours; when the reaction time is 5 hours, the content of ginsenoside Rg3 is higher than the content of ginsenoside Rg5, and is >2 times higher; when the reaction time is 6 hours, the content of ginsenoside Rg3 is lower than the content of ginsenoside Rg5.

[0008] As a preferred embodiment of the present invention, if 30% alcohol-water dilution is used, then in step 2, 300-400 μl of freshly squeezed lemon juice is added to 1 ml of ginseng extract dilution solution, the reaction temperature is controlled at 79°C, the reaction time is controlled at 6 hours, and the content of ginsenoside Rg3 is higher than that of ginsenoside Rg5, controlled at 1.5-1.7 times.

[0009] As a preferred embodiment of the present invention, the method for preparing the ginseng extract is as follows: Step 1. Take the cleaned ginseng root and extract it by hot reflux with 75% ethanol to obtain the ethanol extract; Step 2. After the ethanol extraction is completed, drain the residue, transfer it to the extraction tank, add purified water, and decoct and extract at 100℃ to obtain the aqueous extract. Step 3. Mix the ethanol extract obtained in Step 1 and Step 2 with the water extract, and evaporate to dryness to obtain ginseng extract.

[0010] Advantages and beneficial effects of the present invention: 1. This invention uses commercially available ginseng extract as raw material, which is readily available and suitable for large-scale production.

[0011] 2. Through the summary and analysis of a large number of experiments, this invention found that by using ginseng extract as raw material and by rationally selecting the dilution solvent, controlling the amount of citric acid added, and the reaction temperature and time, it is possible to selectively increase the content of a certain saponin while ensuring the increase of the content of ginsenoside Rg3 and ginsenoside Rg5, thereby controlling the ratio of the two within a reasonable range. This is beneficial for providing customized products for different groups of people, thereby improving the efficacy of the products.

[0012] 3. Compared with traditional high-temperature hydrolysis with strong acids, the conditions of this invention are relatively mild, which helps to reduce side reactions, improve product safety, and avoid the safety risks and equipment corrosion problems caused by the use of strong acids.

[0013] 4. The ginseng extract with high Rg3 and Rg5 content obtained by this invention can be directly applied to health foods, cosmetics, or as a pharmaceutical intermediate, with high added value.

[0014] 5. This invention provides a practical processing method for obtaining ginseng extract products with high Rg3 and Rg5 content. The entire process is simple, the equipment requirements are conventional, and it is easy to scale up for industrial production. Detailed Implementation

[0015] The following detailed description, in conjunction with embodiments, illustrates a method for processing ginseng extract suitable for industrial production and selectively increasing the content of Rg3 and Rg5 according to the present invention. However, those skilled in the art will understand that the following embodiments are not intended to limit the scope of protection of the present invention, and any improvements and variations made based on the present invention are within the scope of protection of the present invention.

[0016] I. Experimental Equipment, Raw Materials and Reagents 1. Experimental equipment (1) Magnetic stirring water bath and corresponding stirring rotor; (2) Acid conversion bottle (using sealed glass bottle with cap); (3) Rotary evaporator (for sample concentration preparation); (4) Pipette; (5) Development tank (for TLC plate running); (6) Alcohol lamp; (7) Hair dryer; (8) Large-size petri dish (for immersing TLC plates); (9) Weighing balance; (10) Flat-mouth tweezers; (11) pH meter; (12) Ultrasonic oscillator; (13) Cutting knife and pad (for cutting TLC plates).

[0017] 2. Experimental materials and reagents (1) 5-year-old cultivated ginseng; (2) fruit (lemon); (3) ginsenoside Rg3 and Rg5 standards; (4) TLC plate (model: Merck TLC Silica gel 60 F254); (5) n-Butanol (analytical grade); (6) TLC plate developing solution (chloroform:methanol:water = 65:35:10); (7) color reagent (sulfuric acid: concentration 5%-10%); (8) pipette tips (1ml, 200μl, 10μl, 5μl); (9) wastewater ethanol (food grade); (10) purified water.

[0018] II. Preparation of Experimental Materials and Reagents 1. Preparing the juice (lemon): Squeeze fresh lemons to collect the juice, test and record the pH value of the lemon juice (2.2-2.4), and keep it for later use.

[0019] Preparation of ginseng extract: Step 1. Ethanol extraction stage: Take 5-year-old cultivated ginseng (cleaned) medicinal materials and place them in a multi-functional extraction tank. Add 75% (v / v) food-grade ethanol, equivalent to 5 times the weight of the medicinal materials, as a solvent. Perform hot reflux extraction at 80℃±2℃ for 2 hours. After extraction, collect the first ethanol extract. Repeat the above extraction operation twice, using the same volume of solvent of the same specification each time. Combine the three ethanol extracts, filter, and set aside.

[0020] Step 2. Water extraction stage: After ethanol extraction, the residue is drained and transferred to an extraction tank. Purified water, equivalent to six times the weight of the original medicinal material, is added. The mixture is boiled at 100°C for 2 hours, and the first aqueous extract is collected. The extraction is repeated once, and the two aqueous extracts are combined, filtered, and set aside.

[0021] Step 3. Mix the ethanol extract obtained in Step 1 and Step 2 with the water extract, and evaporate to dryness to obtain ginseng extract.

[0022] It should be noted that ginseng extract is now produced on a large scale, and in actual preparation, commercially available ginseng extract can be purchased directly.

[0023] 3. Dilute ginseng extract Prepare a diluted ginseng extract solution (also known as ginseng concentrate) by diluting 1g of ginseng extract with 25ml of purified water, 70% alcohol-water solution, or 30% alcohol-water solution. The formula is: Solvent volume (ml) = Ginseng extract (g) × 25 ml.

[0024] 4. Preparation of water-saturated n-butanol Mix purified water and n-butanol thoroughly in a container, and take the upper layer of water saturated with n-butanol for later use. Total volume: 500ml. For long-term use.

[0025] 5. Preparation of developing solution for TLC plates Using a graduated cylinder, prepare the developing solution according to the ratio of chloroform:methanol:distilled water of 65:35:10. Pour the solution into a separatory funnel, mix thoroughly, and let stand until the layers are completely separated and clear. Take the lower layer of liquid for later use. Total volume: 200-300ml.

[0026] 6. Preparation of colorimetric reagent Prepare a 5%-10% concentration solution based on the concentration of concentrated sulfuric acid used (it must be emphasized that the concentrated sulfuric acid must be poured into the water slowly, not the other way around), and set aside. Total preparation volume: 50ml.

[0027] 7. Preparation of ethanol and water-based solvents Prepare solvents (70% alcohol-water or 30% alcohol-water) with 70% and 30% ethanol content using anhydrous ethanol and purified water, respectively, for later use.

[0028] III. Experimental Methods Example 1: Processing of Sample 1 Take 1g of ginseng extract, add 25ml of purified water, stir to dissolve, and obtain water-diluted ginseng concentrate.

[0029] In a 4ml sealed glass bottle with a cap, add 1ml of water to dilute the ginseng concentrate, then add 100μl of freshly squeezed lemon juice. Place the bottle in a magnetic stirrer. Secure the bottle in an 85℃ constant temperature water bath, ensuring the liquid is completely submerged while the bottle opening is above the water level. React at 300 rpm for 4 hours.

[0030] After the reaction was complete, 1 ml of water-saturated n-butanol was added to the glass bottle, and the mixture was shaken thoroughly for extraction. After standing and separating the layers, the n-butanol layer was collected for later use.

[0031] High-performance liquid chromatography (HPLC) was used to analyze the n-butanol extract. The chromatographic conditions were as follows: Syncronis C18 column (100 mm × 2.1 mm, 1.7 μm); mobile phase: acetonitrile-water (22:78, v / v); column temperature: 30 °C; detection wavelength: 203 nm; flow rate: 0.2 mL / min. Using the external standard method, the content of ginsenoside Rg3 in the transformed product was determined to be 0.56 mg / mL, and the content of ginsenoside Rg5 was 0.28 mg / mL. Compared with the untreated ginseng concentrate (Rg3: 0.017 mg / mL, Rg5: 0.014 mg / mL), the content of Rg3 increased by approximately 32 times, and the content of Rg5 increased by approximately 19 times.

[0032] Example 2: Processing of Sample 2 Take 1g of ginseng extract, add 25ml of purified water, stir to dissolve, and obtain water-diluted ginseng concentrate.

[0033] In a 4ml sealed glass bottle with a cap, add 1ml of water to dilute the ginseng concentrate, then add 100μl of freshly squeezed lemon juice, and place the bottle into a magnetic stirrer. Secure the bottle in a 95℃ constant temperature water bath, ensuring the liquid is completely submerged and the bottle opening is above the water level. React at 300 rpm for 3 hours.

[0034] After the reaction was complete, 1 ml of water-saturated n-butanol was added to the glass bottle, and the mixture was shaken thoroughly for extraction. After standing and separating the layers, the n-butanol layer was collected for later use.

[0035] The analysis was performed under the same high-performance liquid chromatography (HPLC) conditions as in Example 1. The content of ginsenoside Rg3 in the transformed product was found to be 0.79 mg / ml, and the content of ginsenoside Rg5 was 0.34 mg / ml. These conditions were among the highest combined contents of Rg3 and Rg5 in all examples, indicating high conversion efficiency. Compared to the starting material, the content of Rg3 increased by approximately 45 times, and the content of Rg5 increased by approximately 23 times.

[0036] This invention also attempted to increase or decrease the amount of lemon juice in the water-diluted ginseng concentrate, for example, adding 150 μl of lemon juice, reacting at 90°C for 4 hours. However, TLC analysis showed that under other conditions, the content of ginsenoside Rg3 and ginsenoside Rg5 could not be effectively increased, so HPLC analysis was not performed subsequently. Furthermore, when adjusting process parameters to increase the content of ginsenoside Rg3 and ginsenoside Rg5 based on the water-diluted ginseng concentrate, the content of ginsenoside Rg3 was consistently higher than that of ginsenoside Rg5, and ≥2 times higher. To achieve a selective increase in the content of ginsenoside Rg3 and ginsenoside Rg5, this invention continued with the following experiments: Example 3: Processing method of sample 3 Take 1g of ginseng extract, add 25ml of 70% ethanol aqueous solution, stir to dissolve, and prepare 70% ethanol-water diluted ginseng concentrate.

[0037] Add 1 ml of the above-mentioned 70% alcohol-water diluted ginseng concentrate to a 4 ml sealed glass bottle with a cap, then add 300 μl of freshly squeezed lemon juice, and place the bottle into a magnetic stirrer. Fix the glass bottle in a 75°C constant temperature water bath, ensuring that the liquid surface is completely submerged in the water bath and the bottle opening is above the water level. React at a stirring speed of 300 rpm for 5 hours.

[0038] After the reaction was complete, the reaction solution was transferred and the ethanol was removed by rotary evaporation at 40°C. 1 ml of purified water was added to the evaporated residue to reconstitute it, followed by 1 ml of water-saturated n-butanol. The mixture was then thoroughly shaken and extracted. After standing and separating the layers, the n-butanol layer was collected for later use.

[0039] The analysis was performed under the same high-performance liquid chromatography (HPLC) conditions as in Example 1. The content of ginsenoside Rg3 in the transformed product was determined to be 0.73 mg / ml, and the content of ginsenoside Rg5 was 0.31 mg / ml. Compared with the raw material, the contents of both Rg3 and Rg5 were significantly increased.

[0040] Example 4: Processing method of sample 4 The difference from Example 3 is that the amount of lemon juice added was 300 μl. Finally, the content of ginsenoside Rg3 in the transformed product was measured to be 0.76 mg / ml, and the content of ginsenoside Rg5 was 0.33 mg / ml.

[0041] Example 5: Processing method of sample 5 Take 1g of ginseng extract, add 25ml of 70% ethanol aqueous solution, stir to dissolve, and prepare 70% ethanol-water diluted ginseng concentrate.

[0042] Add 1 ml of the above-mentioned 70% alcohol-water dilution of ginseng concentrate to a 4 ml sealed glass bottle with a cap, then add 300 μl of freshly squeezed lemon juice, and place the bottle into a magnetic stirrer. Fix the glass bottle in a 75°C constant temperature water bath, ensuring that the liquid surface is completely submerged in the water bath and the bottle opening is above the water level. React at a stirring speed of 300 rpm for 6 hours.

[0043] After the reaction was complete, the reaction solution was transferred and the ethanol was removed by rotary evaporation at 40°C. 1 ml of purified water was added to the evaporated residue to reconstitute it, followed by 1 ml of water-saturated n-butanol. The mixture was then thoroughly shaken and extracted. After standing and separating the layers, the n-butanol layer was collected for later use.

[0044] The analysis was performed under the same high-performance liquid chromatography (HPLC) conditions as in Example 1. The content of ginsenoside Rg3 in the transformed product was determined to be 0.314 mg / ml, and the content of ginsenoside Rg5 was 0.483 mg / ml. Notably, based on a 70% alcohol-water dilution of ginseng concentrate, extending the reaction time effectively controlled the contents of Rg3 and Rg5, resulting in a higher yield of Rg5 than Rg3. Compared to the raw material, the Rg5 content increased by approximately 33 times.

[0045] This invention also attempted to increase or decrease the amount of lemon juice and adjust the reaction time and temperature using a 70% alcohol-water diluted ginseng concentrate. For example, the lemon juice addition was 400 μl, the reaction temperature was 75℃, and the reaction time was 6 h. However, TLC analysis showed that under other conditions, the content of ginsenoside Rg3 and ginsenoside Rg5 could not be effectively increased, so HPLC analysis was not performed subsequently. Furthermore, when adjusting process parameters to increase the content of ginsenoside Rg3 and ginsenoside Rg5 based on a 70% alcohol-water diluted ginseng concentrate, the content of ginsenoside Rg3 was either higher than the content of ginsenoside Rg5 (>2 times) or lower than the content of ginsenoside Rg5. To achieve a relatively balanced content of ginsenoside Rg3 and ginsenoside Rg5, this invention continued with the following experiments: Example 6: Processing method of sample 6 Take 1g of ginseng extract, add 25ml of 30% ethanol aqueous solution, stir to dissolve, and prepare 30% ethanol-water diluted ginseng concentrate.

[0046] Add 1 ml of the above-mentioned 30% alcohol-water dilution of ginseng concentrate to a 4 ml sealed glass bottle with a cap, then add 300 μl of freshly squeezed lemon juice, and place the bottle into a magnetic stirrer. Fix the glass bottle in a 79°C constant temperature water bath, ensuring that the liquid surface is completely submerged in the water bath and the bottle opening is above the water level. React at a stirring speed of 300 rpm for 6 hours.

[0047] After the reaction was complete, the reaction solution was transferred and the ethanol was removed by rotary evaporation at 40°C. 1 ml of purified water was added to the evaporated residue to reconstitute it, followed by 1 ml of water-saturated n-butanol. The mixture was then thoroughly shaken and extracted. After standing and separating the layers, the n-butanol layer was collected for later use.

[0048] The analysis was performed under the same high-performance liquid chromatography (HPLC) conditions as in Example 1. The content of ginsenoside Rg3 in the transformed product was determined to be 0.42 mg / ml, and the content of ginsenoside Rg5 was 0.26 mg / ml. Compared with the raw material, the contents of Rg3 and Rg5 were significantly increased.

[0049] Example 7: Processing method of sample 7 Take 1g of ginseng extract, add 25ml of 30% ethanol aqueous solution, stir to dissolve, and prepare 30% ethanol-water diluted ginseng concentrate.

[0050] Add 1 ml of the above-mentioned 30% alcohol-water dilution of ginseng concentrate to a 4 ml sealed glass bottle with a cap, then add 400 μl of freshly squeezed lemon juice, and place the bottle into a magnetic stirrer. Fix the glass bottle in a 79°C constant temperature water bath, ensuring that the liquid surface is completely submerged in the water bath and the bottle opening is above the water level. React at a stirring speed of 300 rpm for 6 hours.

[0051] After the reaction was complete, the reaction solution was transferred and the ethanol was removed by rotary evaporation at 40°C. 1 ml of purified water was added to the evaporated residue to reconstitute it, followed by 1 ml of water-saturated n-butanol. The mixture was then thoroughly shaken and extracted. After standing and separating the layers, the n-butanol layer was collected for later use.

[0052] The analysis was performed under the same high-performance liquid chromatography conditions as in Example 1. The content of ginsenoside Rg3 in the transformation product was found to be 0.58 mg / ml, and the content of ginsenoside Rg5 was found to be 0.372 mg / ml.

[0053] This invention also explored increasing or decreasing the amount of lemon juice and adjusting the reaction time and temperature under a 30% alcohol-water dilution of ginseng concentrate. For example, the lemon juice addition was 200 μl, the reaction temperature was 75°C, and the reaction time was 5 h; the lemon juice addition was 300 μl, the reaction temperature was 75°C, and the reaction time was 5 h. However, TLC analysis revealed that under other conditions, the content of ginsenoside Rg3 and ginsenoside Rg5 could not be effectively increased, so HPLC analysis was not performed subsequently. Moreover, when adjusting the process parameters to increase the content of ginsenoside Rg3 and ginsenoside Rg5 based on a 30% alcohol-water dilution of ginseng concentrate, the content of ginsenoside Rg3 was slightly higher than that of ginsenoside Rg5, which could be controlled at approximately 1.5 times.

[0054] In addition to the above-mentioned experiments, this invention also explored other diluents and acids (tartaric acid, hydrochloric acid, etc.). However, it was found that by using ginseng extract as raw material and rationally selecting diluents, controlling the amount of citric acid added, and adjusting the reaction temperature and time, it is possible to selectively increase the content of a certain saponin while ensuring the increase of the content of ginsenoside Rg3 and ginsenoside Rg5, thereby controlling the ratio of the two within a reasonable range. This is beneficial for providing customized products for different groups of people, thereby improving the therapeutic effect.

[0055] The above describes specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A processing method for ginseng extract suitable for industrial-scale production that can selectively increase the content of Rg3 and Rg5, characterized in that, The method includes the following steps: Step 1. Take ginseng extract and dilute it with purified water, 70% alcohol water, or 30% alcohol water according to the required ratio of Rg3 and Rg5 to prepare a diluted ginseng extract solution. Step 2. Add the diluted ginseng extract solution to a sealed glass bottle with a lid, add freshly squeezed lemon juice, put in a magnetic stirrer, fix the glass bottle in a constant temperature water bath, and stir to react. Step 3. After the reaction is complete, the product is extracted with water-saturated n-butanol, concentrated and dried to obtain a product rich in ginsenosides Rg3 and Rg5 in a fixed ratio.

2. The ginseng extract processing method according to claim 1, which is suitable for industrial production and can selectively increase the content of Rg3 and Rg5, is characterized in that, Take 1g of ginseng extract and dilute it with 25ml of purified water, 70% alcohol water, or 30% alcohol water according to the required Rg3 and Rg5 ratio to prepare a diluted ginseng extract solution.

3. The ginseng extract processing method according to claim 2, which is suitable for industrial production and can selectively increase the content of Rg3 and Rg5, is characterized in that... If purified water is used for dilution, 100 μl of freshly squeezed lemon juice is added to 1 ml of ginseng extract dilution solution in step 2. The reaction temperature is controlled at 85℃-95℃, and the reaction time is controlled at 3-4 hours. The content of ginsenoside Rg3 is higher than that of ginsenoside Rg5, and is ≥2 times higher.

4. The ginseng extract processing method according to claim 2, which is suitable for industrial production and can selectively increase the content of Rg3 and Rg5, is characterized in that... If 70% alcohol-water dilution is used, then in step 2, add 200-300 μl of freshly squeezed lemon juice to 1 ml of ginseng extract dilution solution, control the reaction temperature at 75℃, and control the reaction time at 5-6 hours; when the reaction time is 5 hours, the content of ginsenoside Rg3 is higher than the content of ginsenoside Rg5, and is >2 times higher; when the reaction time is 6 hours, the content of ginsenoside Rg3 is lower than the content of ginsenoside Rg5.

5. A method for processing ginseng extract suitable for industrial production and selectively increasing the content of Rg3 and Rg5, as described in claim 2, is characterized in that... If 30% alcohol-water dilution is used, add 300-400μl of freshly squeezed lemon juice to 1ml of ginseng extract dilution solution in step 2. Control the reaction temperature at 79℃ and the reaction time at 6 hours. The content of ginsenoside Rg3 is higher than that of ginsenoside Rg5, and is controlled at 1.5-1.7 times.

6. A method for processing ginseng extract suitable for industrial production and selectively increasing the content of Rg3 and Rg5, as described in claim 2, is characterized in that... The preparation method of the ginseng extract is as follows: Step 1. Take the cleaned ginseng root and extract it by hot reflux with 75% ethanol to obtain the ethanol extract; Step 2. After the ethanol extraction is completed, drain the residue, transfer it to the extraction tank, add purified water, and decoct and extract at 100℃ to obtain the aqueous extract. Step 3. Mix the ethanol extract obtained in Step 1 and Step 2 with the water extract, and evaporate to dryness to obtain ginseng extract.