Rose essential oil microencapsulated composition for improving sleep as well as preparation method and application of rose essential oil microencapsulated composition

Abstract

The invention discloses a rose essential oil microencapsulated composition for improving sleep as well as a preparation method and application of the rose essential oil microencapsulated composition, and belongs to the technical field of pharmaceutical preparations. The composition is prepared from the following components in parts by weight: 10 to 15 parts of rose essential oil, 2 to 6 parts of peony seed oil, 12 to 20 parts of octenyl succinic acid dextrin, 1 to 5 parts of freshly squeezed olive oil, 0.3 to 1 part of acer truncatum seed oil, 8 to 15 parts of beta-cyclodextrine, 1 to 5 parts of sodium ascorbate, 0.5 to 1 part of sleep-aiding raw materials, 5 to 15 parts of Arabic gum and 100 to 150 parts of water. Compared with the prior art, the problems that the rose essential oil is easy to volatilize and unstable and the bioavailability of various active ingredients is low are effectively solved, targeted efficient release of the active ingredients in the intestinal tract is achieved, the sleep improving effect is remarkably improved, the product safety is high, and the rose essential oil can be applied to preparation of drugs for improving sleep.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical preparation technology, and in particular to a microencapsulated composition of rose essential oil for improving sleep, its preparation method and application. Background Technology

[0002] Rose essential oil, as a natural active ingredient, is rich in various functional substances such as alcohols and esters, demonstrating unique value in mood soothing and sleep improvement, and related research and applications have continued to expand in recent years. However, the volatility and poor thermal stability of rose essential oil components lead to significant loss of activity during processing and storage, and its bioavailability is low when taken directly. These issues greatly limit its large-scale application in sleep aid products.

[0003] Microencapsulation technology offers an effective solution to the stability issues of essential oil components. By selecting appropriate wall materials to encapsulate the core material, the loss of active ingredients can be significantly reduced, and the sustained-release and controlled-release of active substances can be achieved. Currently, various microencapsulated compositions for essential oils have been developed, covering multiple fields such as food and health products. However, most studies focus only on the encapsulation of single essential oils, and research on the synergistic encapsulation and efficacy enhancement of essential oils with other sleep-aiding active ingredients is still relatively lacking.

[0004] While existing patented technologies have explored related areas, they still have significant limitations. Patent CN113730299A proposes a microcapsule preparation process for essential oils, which can achieve targeted sustained release of active ingredients, but it does not optimize the formulation for sleep-aiding effects, limiting the sleep-aiding effect of a single essential oil. Patent CN115812939A discloses a microcapsule for Sichuan pepper oil, focusing on improving the high-temperature resistance and storage stability of the essential oil, but it does not involve the compounding of sleep-aiding active ingredients. Patent CN115428930A mentions the application of sleep-aiding plant extracts, but it does not combine microencapsulation technology to address the issues of ingredient stability and release efficiency.

[0005] In summary, current technologies either focus solely on improving the stability of essential oils or emphasize the application of a single sleep-aiding ingredient, failing to achieve precise blending and synergistic encapsulation of rose essential oil with multiple sleep-aiding ingredients. Furthermore, existing microencapsulation processes often suffer from insufficient encapsulation efficiency and mismatches between active ingredient release and human sleep regulation patterns, making it difficult to fully leverage the synergistic effects of rose essential oil and sleep-aiding ingredients. Therefore, developing a microencapsulated composition that simultaneously addresses the issues of poor rose essential oil stability, weak synergy of sleep-aiding components, and low precision in active ingredient release has become a crucial technological breakthrough in the current field of sleep-aiding products. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention aims to provide a microencapsulated rose essential oil composition for improving sleep, its preparation method, and its application.

[0007] To achieve the above-mentioned objectives, the present invention adopts the following technical solution:

[0008] A microencapsulated composition of rose essential oil for improving sleep, comprising the following components in parts by weight:

[0009] 10-15 parts rose essential oil, 2-6 parts peony seed oil, 12-20 parts octenyl succinic dextrin, 1-5 parts freshly pressed olive oil, 0.3-1 part Acer truncatum seed oil, 8-15 parts β-cyclodextrin, 1-5 parts sodium ascorbate, 0.5-1 part sleep aid ingredient, 5-15 parts gum arabic, 100-150 parts water.

[0010] The sleep-aiding ingredients are at least one of the following: jujube seed extract, longan pulp extract, wolfberry polysaccharide, rosemary extract, γ-aminobutyric acid, valerian extract, poria cocos polysaccharide extract, blueberry extract, and hops extract.

[0011] Preferably, the sleep-aiding ingredients are composed of valerian extract and hops extract in a mass ratio of 2-4:1-3.

[0012] The method for preparing the sleep-improving rose essential oil microencapsulated composition is as follows:

[0013] Step 1: Add rose essential oil and peony seed oil to a sealed container and stir in a constant temperature water bath to obtain an oil phase mixture;

[0014] Step 2: Add octenyl succinic dextrin, freshly pressed olive oil, Acer truncatum seed oil, β-cyclodextrin, and sodium ascorbate to water, heat and stir, then add the sleep aid ingredients and continue stirring for 5-15 minutes to obtain an aqueous mixture;

[0015] Step 3: High-speed shearing. Slowly pump the aqueous phase mixture into the oil phase mixture at a rate of 1-1.5 mL / s. After emulsification, add gum arabic and reduce the speed to 100-150 r / min. Continue stirring until the gum arabic is completely dissolved to form a rose essential oil complex emulsion.

[0016] Step 4: Homogenize the rose essential oil complex emulsion under high pressure, spray dry the homogenized emulsion, and collect the dried product to obtain the rose essential oil microencapsulated composition for improving sleep.

[0017] Preferably, the method for preparing the sleep-improving rose essential oil microencapsulated composition is as follows:

[0018] Step 1: Add rose essential oil, peony seed oil and sleep aid ingredients to a sealed container, place it in a constant temperature water bath and stir to obtain an oil phase mixture;

[0019] Step 2: Add octenyl succinic dextrin, freshly pressed olive oil, Acer truncatum seed oil, β-cyclodextrin, and sodium ascorbate to water, heat and stir, then continue stirring for 5-15 minutes to obtain an aqueous mixture;

[0020] Step 3: High-speed shearing. Slowly pump the aqueous phase mixture into the oil phase mixture at a rate of 1-1.5 mL / s. After emulsification, add gum arabic and reduce the speed to 100-150 r / min. Continue stirring until the gum arabic is completely dissolved to form a rose essential oil complex emulsion.

[0021] Step 4: Homogenize the rose essential oil complex emulsion under high pressure, spray dry the homogenized emulsion, and collect the dried product to obtain the rose essential oil microencapsulated composition for improving sleep.

[0022] A further preferred embodiment is the preparation method of the sleep-improving rose essential oil microencapsulated composition as follows:

[0023] Step 1: Add rose essential oil, peony seed oil, sleep aid ingredients and β-cyclodextrin to a sealed container, place it in a constant temperature water bath and stir to obtain an oil phase mixture;

[0024] Step 2: Add octenyl succinic dextrin, freshly pressed olive oil, Acer truncatum seed oil, and sodium ascorbate to water, heat and stir, then continue stirring for 5-15 minutes to obtain an aqueous mixture;

[0025] Step 3: High-speed shearing. Slowly pump the aqueous phase mixture into the oil phase mixture at a rate of 1-1.5 mL / s. After emulsification, add gum arabic and reduce the speed to 100-150 r / min. Continue stirring until the gum arabic is completely dissolved to form a rose essential oil complex emulsion.

[0026] Step 4: Homogenize the rose essential oil complex emulsion under high pressure, spray dry the homogenized emulsion, and collect the dried product to obtain the rose essential oil microencapsulated composition for improving sleep.

[0027] In step 1, the stirring in the constant temperature water bath environment is carried out by placing the mixture in a constant temperature water bath environment of 40-60℃ and stirring at a speed of 80-150r / min for 10-20 minutes.

[0028] In step 2, the heating and stirring process involves heating the temperature to 50-70°C and then stirring at a speed of 100-400 r / min for 20-60 minutes to dissolve the material.

[0029] In step 3, the high-speed shearing speed is 6000-8000 r / min, and the emulsification time is 2-8 minutes.

[0030] In step 4, high-pressure homogenization involves performing 1-3 cycles of homogenization at a pressure of 40-60 MPa.

[0031] In step 4, the spray drying process involves setting the inlet air temperature to 160-175℃, the outlet air temperature to 70-85℃, and controlling the feed flow rate to 5-10mL / min.

[0032] The application of the microencapsulated rose essential oil composition for improving sleep in the preparation of pharmaceuticals for improving sleep.

[0033] The design of this invention focuses on improving sleep quality. It first examines the compatibility of single sleep-aiding ingredients. By comparing the effects of different ingredients with the core essential oil components, it initially identifies the types of ingredients that can play an auxiliary role, laying a solid foundation for subsequent formula optimization.

[0034] Building upon the screening of individual ingredients, the R&D team further explored the synergistic effects between ingredients. By compounding different types of sleep-aiding ingredients and leveraging their complementary mechanisms, they enhanced the overall sleep improvement capabilities, overcoming the efficacy bottleneck of single ingredients and making the sleep-aiding performance of the composition more stable and efficient.

[0035] To further unleash the synergistic potential of the raw materials, the R&D team began optimizing the preparation process and adjusting the addition steps of the sleep-aiding ingredients to allow the active ingredients to more fully integrate with the core components and reduce efficacy loss during the preparation process. Subsequently, they continued to optimize the timing of adding related raw materials to further improve the encapsulation effect of the system, reduce the activity loss of the core components, and make the release of active ingredients more in line with human needs, ultimately achieving a precise match between the preparation process and the formulation.

[0036] Compared with the prior art, the present invention has the following beneficial technical effects:

[0037] 1) This invention, through the compounding of suitable sleep-aiding ingredients, can synergistically regulate the central nervous system pathways through multiple pathways. Compared with a single sleep-aiding ingredient, it can more effectively shorten the time to fall asleep and prolong the total sleep time, resulting in a more prominent sleep-aiding effect.

[0038] 2) This invention optimizes the preparation process in multiple stages. By adjusting the timing of adding active raw materials and constructing a multi-layer encapsulation structure, the stability and intestinal release efficiency of rose essential oil and sleep-aiding components are greatly improved, and component loss is effectively reduced.

[0039] 3) The microencapsulated composition of the present invention has been verified by safety testing and has no obvious toxic side effects. It can be widely used in various food and health product carriers, and has both sleep-aiding effects and food safety. Detailed Implementation

[0040] Some material parameters and their sources:

[0041] Octenyl succinic dextrin: degree of substitution 0.03, dextrin DE value 12.

[0042] Peony seed oil: α-linolenic acid content 42.5%, acid value 0.4 mg KOH / g.

[0043] Acer truncatum seed oil: nervonic acid content 5.8%, acid value 0.7mgKOH / g.

[0044] Freshly pressed olive oil: acidity 0.5%, peroxide value 12 meq / kg.

[0045] β-Cyclodextrin: Water solubility 18 g / L (25℃).

[0046] Gum arabic: 9% moisture.

[0047] Jujube seed extract: Jujube seed saponin A content 3.2%.

[0048] Longan pulp extract: extract ratio 10:1.

[0049] Goji berry polysaccharides: Polysaccharide content 42%.

[0050] Rosemary extract: 11% rosmarinic acid.

[0051] Valerian extract: Valerian acid content 0.9%.

[0052] Poria cocos polysaccharide extract: Poria cocos polysaccharide content 16%, moisture 11%.

[0053] Blueberry extract: anthocyanin content 26%.

[0054] Hops extract: Humus content 11%.

[0055] Table 1. Analysis of the main components of rose essential oil

[0056]

[0057] In the embodiments and comparative examples of this invention, all raw materials are commercially available products.

[0058] Example 1

[0059] A method for preparing a microencapsulated composition of rose essential oil for improving sleep is as follows, in parts by weight:

[0060] Step 1: Add 12 parts of rose essential oil and 4 parts of peony seed oil to a sealed container, place it in a 50°C constant temperature water bath, and stir and mix at 120r / min for 15 minutes to obtain an oil phase mixture.

[0061] Step 2: Add 16 parts octenyl succinic dextrin, 3 parts freshly pressed olive oil, 0.8 parts Acer truncatum seed oil, 11 parts β-cyclodextrin, and 2.5 parts sodium ascorbate to 120 parts water. Heat to 60°C and stir at 200 r / min for 40 minutes to dissolve. Then add 0.8 parts sleep aid ingredients and continue stirring for 10 minutes to obtain an aqueous mixture.

[0062] Step 3: Start the high-speed shear machine and adjust the speed to 6800 r / min. Slowly pump the aqueous phase mixture into the oil phase mixture at a rate of 1.2 mL / s. After emulsifying for 5 minutes, add 10 parts of gum arabic and reduce the speed to 140 r / min. Continue stirring until the gum arabic is completely dissolved to form a rose essential oil complex emulsion.

[0063] Step 4: Transfer the rose essential oil complex emulsion into a high-pressure homogenizer and perform two cycles of homogenization under a pressure of 50 MPa. Then, send the homogenized emulsion into a centrifugal spray dryer, set the inlet air temperature to 168°C, the outlet air temperature to 78°C, and the feed flow rate to 8 mL / min. Collect the dried product to obtain the rose essential oil microencapsulated composition for improving sleep.

[0064] The sleep-aiding ingredient is jujube seed extract.

[0065] Example 2

[0066] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is longan pulp extract.

[0067] Example 3

[0068] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is wolfberry polysaccharide.

[0069] Example 4

[0070] The preparation method of a microencapsulated composition of rose essential oil for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is rosemary extract.

[0071] Example 5

[0072] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is γ-aminobutyric acid.

[0073] Example 6

[0074] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is valerian extract.

[0075] Example 7

[0076] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is a Poria cocos polysaccharide extract.

[0077] Example 8

[0078] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is blueberry extract.

[0079] Example 9

[0080] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is hop extract.

[0081] Example 10

[0082] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw materials are composed of valerian extract and hop extract in a mass ratio of 3:2.

[0083] Example 11

[0084] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is composed of Poria cocos polysaccharide extract and Lycium barbarum polysaccharide in a mass ratio of 3:2.

[0085] Example 12

[0086] A method for preparing a microencapsulated composition of rose essential oil for improving sleep is as follows, in parts by weight:

[0087] Step 1: Add 12 parts rose essential oil, 4 parts peony seed oil and 0.8 parts sleep aid ingredients to a sealed container, place it in a 50℃ constant temperature water bath, and stir and mix at 120r / min for 15 minutes to obtain an oil phase mixture.

[0088] Step 2: Add 16 parts of octenyl succinic dextrin, 3 parts of freshly pressed olive oil, 0.8 parts of Acer truncatum seed oil, 11 parts of β-cyclodextrin, and 2.5 parts of sodium ascorbate to 120 parts of water. Heat to 60°C and stir at 200 r / min for 40 minutes to dissolve. Then continue stirring for 10 minutes to obtain an aqueous mixture.

[0089] Step 3: Start the high-speed shear machine and adjust the speed to 6800 r / min. Slowly pump the aqueous phase mixture into the oil phase mixture at a rate of 1.2 mL / s. After emulsifying for 5 minutes, add 10 parts of gum arabic and reduce the speed to 140 r / min. Continue stirring until the gum arabic is completely dissolved to form a rose essential oil complex emulsion.

[0090] Step 4: Transfer the rose essential oil complex emulsion into a high-pressure homogenizer and perform two cycles of homogenization under a pressure of 50 MPa. Then, send the homogenized emulsion into a centrifugal spray dryer, set the inlet air temperature to 168°C, the outlet air temperature to 78°C, and the feed flow rate to 8 mL / min. Collect the dried product to obtain the rose essential oil microencapsulated composition for improving sleep.

[0091] The sleep aid ingredients are the same as those in Example 10.

[0092] Example 13

[0093] A method for preparing a microencapsulated composition of rose essential oil for improving sleep is as follows, in parts by weight:

[0094] Step 1: Add 12 parts of rose essential oil, 4 parts of peony seed oil, 0.8 parts of sleep aid ingredients and 11 parts of β-cyclodextrin to a sealed container, place it in a 50°C constant temperature water bath environment, and stir and mix at 120r / min for 15 minutes to obtain an oil phase mixture.

[0095] Step 2: Add 16 parts of octenyl succinic dextrin, 3 parts of freshly pressed olive oil, 0.8 parts of Acer truncatum seed oil, and 2.5 parts of sodium ascorbate to 120 parts of water. Heat to 60°C and stir at 200 r / min for 40 minutes to dissolve. Then continue stirring for 10 minutes to obtain an aqueous mixture.

[0096] Step 3: Start the high-speed shear machine and adjust the speed to 6800 r / min. Slowly pump the aqueous phase mixture into the oil phase mixture at a rate of 1.2 mL / s. After emulsifying for 5 minutes, add 10 parts of gum arabic and reduce the speed to 140 r / min. Continue stirring until the gum arabic is completely dissolved to form a rose essential oil complex emulsion.

[0097] Step 4: Transfer the rose essential oil complex emulsion into a high-pressure homogenizer and perform two cycles of homogenization under a pressure of 50 MPa. Then, send the homogenized emulsion into a centrifugal spray dryer, set the inlet air temperature to 168°C, the outlet air temperature to 78°C, and the feed flow rate to 8 mL / min. Collect the dried product to obtain the rose essential oil microencapsulated composition for improving sleep.

[0098] The sleep aid ingredients are the same as those in Example 10.

[0099] Comparative Example 1

[0100] The preparation method of a microencapsulated rose essential oil composition for improving sleep is basically the same as that in Example 1, except that the sleep-aiding raw material is replaced with an equal amount of water.

[0101] Test Example 1

[0102] Sleep improvement test:

[0103] Laboratory animal source: SPF-grade male ICR mice, weighing 18-22g, provided by Beijing Vital River Laboratory Animal Technology Co., Ltd.

[0104] Householding environment: Barrier system animal room, housed in individually ventilated cages (IVC).

[0105] Environmental parameters: temperature 22±2℃, relative humidity 55±5%, light cycle 12h / 12h (daytime 08:00-20:00, nighttime 20:00-08:00).

[0106] Husbandry conditions: Mice were allowed free access to standard maintenance diet and water for 7 days to acclimatize. The rose essential oil microencapsulated compositions prepared in the embodiments and comparative examples of this invention were administered to mice by gavage at a dose of 100 mg / kg·d for 14 consecutive days. The blank control group was administered an equal volume of physiological saline by gavage. Eight mice were tested in each group. On the 14th day of administration, each mouse was placed in video monitoring, and its sleep behavior was continuously recorded for 12 hours during the dark period (20:00-08:00 the next day).

[0107] Observation indicator: Total sleep time: The cumulative time spent lying still and in a relaxed posture;

[0108] Sleep latency: The time from the start of the dark phase to the first entry into a sleep state that lasts ≥3 minutes.

[0109] The average value was taken, and the test results are shown in Table 2.

[0110] Table 2

[0111]

[0112] The total sleep time of the single sleep aid ingredient groups (Examples 1-9) increased by 9.1%-19.1% compared with the control group, and the sleep latency was shortened by 20.9%-34.6%. Among them, the groups containing γ-aminobutyric acid, valerian extract, and hops extract had better effects, mainly by regulating the central GABAergic neural pathway and inhibiting nerve excitability.

[0113] The compound group (Example 10) showed significantly better results than the single-ingredient group. In Example 10, the total sleep time increased by 33.1% and the sleep latency was shortened by 52.5% compared with the blank group, demonstrating the synergistic effect of valerian extract and hop extract: valerone from valerian can enhance the affinity of GABA receptors, and hop components can regulate the release of 5-hydroxytryptamine. The two work together to accelerate sleep initiation and prolong sleep time.

[0114] The present invention further enhances the effect through process. Example 12 improves the bioavailability of fat-soluble sleep aid ingredients by oil phase loading. Example 13 further improves the stability of active ingredients and intestinal release efficiency by cyclodextrin pre-encapsulation, thereby significantly prolonging the total sleep time and shortening the sleep latency.

[0115] Test Example 2

[0116] In vitro simulated digestion and release performance test:

[0117] Experimental materials and reagents

[0118] Experimental samples: 1g each of the rose essential oil microencapsulated compositions prepared in Examples 1-13 and Comparative Example 1.

[0119] Reagents: artificial gastric juice (pH 1.2, containing 0.2% pepsin), artificial intestinal juice (pH 7.4, containing 0.6% trypsin + 0.3% bile salts), n-hexane (chromatographic grade), phosphate buffer (0.1 mol / L).

[0120] Test methods

[0121] Simulate the gastric digestion process: Add 1g of sample to 100mL of artificial gastric fluid and shake at 37℃ and 100r / min for 1 hour; immediately remove all samples, centrifuge at 12000r / min for 10 minutes, collect the precipitate (unreleased microcapsules), wash twice with phosphate buffer to remove gastric fluid components adsorbed on the surface.

[0122] The washed precipitate was transferred to 100 mL of artificial intestinal fluid and shaken at 37 °C and 100 r / min for 2 hours. After the extraction, 20 mL of n-hexane was added to extract the released rose essential oil. The extraction was carried out by shaking for 10 minutes and then centrifuged at 12000 r / min for 10 minutes to obtain the n-hexane phase.

[0123] Content determination: The total content of citronellol, a marker component of rose essential oil, in the microencapsulated rose essential oil composition before and after release was determined by GC-MS external standard method, and the release rate was calculated based on the total amount of citronellol added.

[0124] Calculation method: Release rate = (Total content of citronellol extracted from intestinal fluid / Total content of citronellol in microcapsules) × 100%.

[0125] Each experiment was conducted three times, and the average value was taken. The relevant test data are summarized in Table 3.

[0126] Table 3

[0127]

[0128] The release rates of rose essential oil in the conventional process group (Examples 1-11) and Comparative Example 1 were between 78.3% and 81.2%, indicating that the basic microencapsulation process of the present invention can effectively protect rose essential oil from degradation in the stomach and ensure release efficiency in the intestine.

[0129] Example 12 optimized the oil-water interface compatibility by placing the fat-soluble sleep aid ingredient and rose essential oil together in the oil phase, reducing the loss of essential oil volatilization during spray drying and increasing the intestinal fluid release rate to 86.2%.

[0130] Example 13 uses cyclodextrin to pre-encapsulate sleep-aid ingredients and rose essential oil, forming a three-layer encapsulation structure of cyclodextrin, oil phase components, and composite wall material. This further enhances the stability of the essential oil in the stomach and allows for rapid release in the intestinal environment, achieving a release rate of 90.5% and significantly improving the effective utilization rate of the active ingredients in the intestine.

[0131] Test Example 3

[0132] Acute oral toxicity safety test:

[0133] Laboratory animals: SPF-grade ICR mice, half male and half female, weighing 18-22g, 20 mice per group, provided by Beijing Vital River Laboratory Animal Technology Co., Ltd.

[0134] Dosage regimen: 1 g of the microencapsulated compositions of Examples 1-13 and Comparative Example 1 was added to 10 mL of 0.5% CMC-Na aqueous solution and magnetically stirred for 30 minutes to prepare a suspension. The suspension was administered orally at a dose of 5000 mg / kg body weight, while the control group was administered an equal volume of physiological saline by gavage.

[0135] Observation indicators: The mice were observed for 14 consecutive days, and their general condition, weight changes, behavior and mortality were recorded. After the observation period, the surviving mice were dissected to examine the pathological changes of major organs such as the heart, liver, spleen, lungs and kidneys.

[0136] Results explanation:

[0137] No mice in any of the treatment groups died during the 14-day observation period. They were in good general condition, their weight increased normally with the feeding time, and their behavior was normal. No obvious pathological damage was found in any of the major organs after dissection. This indicates that the rose essential oil microencapsulated compositions prepared in all embodiments and comparative examples of this invention are practically non-toxic products with excellent safety.

Claims

1. A microencapsulated composition of rose essential oil for improving sleep, characterized in that, It consists of the following components in parts by weight: 10-15 parts rose essential oil, 2-6 parts peony seed oil, 12-20 parts octenyl succinic dextrin, 1-5 parts freshly pressed olive oil, 0.3-1 part Acer truncatum seed oil, 8-15 parts β-cyclodextrin, 1-5 parts sodium ascorbate, 0.5-1 part sleep aid ingredient, 5-15 parts gum arabic, 100-150 parts water; The sleep-aiding ingredients are composed of valerian extract and hops extract in a mass ratio of 2-4:1-3.

2. A method for preparing the rose essential oil microencapsulated composition for improving sleep as described in claim 1, characterized in that, The method is as follows: Step 1: Add rose essential oil and peony seed oil to a sealed container and stir in a constant temperature water bath to obtain an oil phase mixture; Step 2: Add octenyl succinic dextrin, freshly pressed olive oil, Acer truncatum seed oil, β-cyclodextrin, and sodium ascorbate to water, heat and stir, then add the sleep aid ingredients and continue stirring for 5-15 minutes to obtain an aqueous mixture; Step 3: High-speed shearing. Slowly pump the aqueous phase mixture into the oil phase mixture at a rate of 1-1.5 mL / s. After emulsification, add gum arabic and reduce the speed to 100-150 r / min. Continue stirring until the gum arabic is completely dissolved to form a rose essential oil complex emulsion. Step 4: Homogenize the rose essential oil complex emulsion under high pressure, spray dry the homogenized emulsion, and collect the dried product to obtain the rose essential oil microencapsulated composition for improving sleep.

3. A method for preparing the rose essential oil microencapsulated composition for improving sleep as described in claim 1, characterized in that, The method is as follows: Step 1: Add rose essential oil, peony seed oil and sleep aid ingredients to a sealed container, place it in a constant temperature water bath and stir to obtain an oil phase mixture; Step 2: Add octenyl succinic dextrin, freshly pressed olive oil, Acer truncatum seed oil, β-cyclodextrin, and sodium ascorbate to water, heat and stir, then continue stirring for 5-15 minutes to obtain an aqueous mixture; Step 3: High-speed shearing. Slowly pump the aqueous phase mixture into the oil phase mixture at a rate of 1-1.5 mL / s. After emulsification, add gum arabic and reduce the speed to 100-150 r / min. Continue stirring until the gum arabic is completely dissolved to form a rose essential oil complex emulsion. Step 4: Homogenize the rose essential oil complex emulsion under high pressure, spray dry the homogenized emulsion, and collect the dried product to obtain the rose essential oil microencapsulated composition for improving sleep.

4. A method for preparing the rose essential oil microencapsulated composition for improving sleep as described in claim 1, characterized in that, The method is as follows: Step 1: Add rose essential oil, peony seed oil, sleep aid ingredients and β-cyclodextrin to a sealed container, place it in a constant temperature water bath and stir to obtain an oil phase mixture; Step 2: Add octenyl succinic dextrin, freshly pressed olive oil, Acer truncatum seed oil, and sodium ascorbate to water, heat and stir, then continue stirring for 5-15 minutes to obtain an aqueous mixture; Step 3: High-speed shearing. Slowly pump the aqueous phase mixture into the oil phase mixture at a rate of 1-1.5 mL / s. After emulsification, add gum arabic and reduce the speed to 100-150 r / min. Continue stirring until the gum arabic is completely dissolved to form a rose essential oil complex emulsion. Step 4: Homogenize the rose essential oil complex emulsion under high pressure, spray dry the homogenized emulsion, and collect the dried product to obtain the rose essential oil microencapsulated composition for improving sleep.

5. The method according to any one of claims 2-4, characterized in that, In step 1, the stirring in the constant temperature water bath environment is carried out by placing the mixture in a constant temperature water bath environment of 40-60℃ and stirring at a speed of 80-150r / min for 10-20 minutes.

6. The method according to any one of claims 2-4, characterized in that, In step 2, the heating and stirring process involves heating the temperature to 50-70°C and then stirring at a speed of 100-400 r / min for 20-60 minutes to dissolve the material.

7. The method according to any one of claims 2-4, characterized in that, In step 3, the high-speed shearing speed is 6000-8000 r / min, and the emulsification time is 2-8 minutes.

8. The method according to any one of claims 2-4, characterized in that, In step 4, high-pressure homogenization involves performing 1-3 cycles of homogenization at a pressure of 40-60 MPa.

9. The method according to any one of claims 2-4, characterized in that, In step 4, the spray drying process involves setting the inlet air temperature to 160-175℃, the outlet air temperature to 70-85℃, and controlling the feed flow rate to 5-10mL / min.

10. The use of the sleep-improving rose essential oil microencapsulated composition as described in claim 1 in the preparation of a sleep-improving pharmaceutical product.

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