Sphingobacterium, microbial inoculum, method and application thereof

Abstract

The invention discloses a sphingobacterium bacterium, a bacterium agent, a method and application of the sphingobacterium bacterium. The invention provides a sphingobacterium sp. QY5 strain, the preservation number of the sphingobacterium sp. QY5 strain is CCTCC (China Center for Type Culture Collection) NO: M 2026053, and the preservation number of the sphingobacterium sp. QY5 strain is CCTCC NO: M 2026053. Also provided are cultures or compositions of the strain. Experiments prove that the strain QY5 shows remarkable degradation potential on the polypropylene plastic: the strain QY5 can grow by taking polypropylene as a unique carbon source, the strain can tolerate the concentration of the polypropylene plastic as high as 1000 mg / L, after the strain acts on the strain QY5, infrared spectrum characteristic functional groups of the polypropylene and characteristic products of a differential scanning calorimetry are remarkably reduced, and the degradation potential of the polypropylene plastic is remarkably improved. And the thermodynamic property of the material is changed. The strain can be used for degrading polypropylene plastic residues and environmental loads in various environments, enriches a polypropylene degrading strain library, and has wide application potential and market value.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology and relates to Sphingosporobacter spp., bacterial agents, methods and their applications. Background Technology

[0002] Polypropylene (PP) is a semi-crystalline thermoplastic. It possesses high impact resistance, strong mechanical properties, and resistance to various organic solvents and acid / alkali corrosion. It has wide applications in industry and is one of the most common polymer materials. In daily life, it is widely used in food packaging, such as microwaveable disposable tableware containers, elastic or rigid food bags, as well as pipes and parts.

[0003] Microorganisms play a crucial role in decomposing foreign substances, and microbial degradation is considered an environmentally friendly treatment method. However, due to the presence of methyl groups on the side chains of polypropylene (PP), it exhibits strong hydrophobicity and low bioavailability, making it more difficult to degrade than other plastics. Currently, few bacteria have been found to degrade polypropylene, and further screening is needed to identify more PP-degrading bacteria. Therefore, screening for more PP plastic-degrading bacteria holds broad application prospects. Summary of the Invention

[0004] The technical problem solved by this invention is to obtain new polypropylene-degrading bacteria and identify their functions.

[0005] To solve the above-mentioned technical problems, the first aspect of the present invention provides *Sphingomonas* spp. ( Sphingobacterium The strain QY5 (sp.) has the accession number CCTCC NO: M 2026053.

[0006] In the above text, the genus *Sphingosporobacter* ( Sphingobacterium The QY5 strain (sp.) or its variants or descendants are also within the scope of protection of this invention.

[0007] The above-mentioned *Sphingosporobacter* genus ( Sphingobacterium (sp.) variants of strain QY5 or sphingomonas genus ( Sphingobacterium The progeny of strain QY5 (sp.) and the genus *Sphingosporobacter* (sp.) Sphingobacterium The QY5 strain (sp.) has similar or identical functions.

[0008] In the above text, the similar or identical function is the degradation of polypropylene.

[0009] In a second aspect, the present invention provides a culture of the QY5 strain described in the first aspect, which is a substance obtained by culturing the QY5 strain described in the first aspect in a microbial culture medium.

[0010] In the cultures described above, the microbial culture medium can be a solid culture medium or a liquid culture medium.

[0011] The term "culture" refers to a liquid or solid product (all substances within the culture vessel are fermentation products) that has grown a microbial community after artificial inoculation and cultivation. It is a product obtained by growing and / or amplifying microorganisms; it can be a biologically pure culture of microorganisms, or it can contain a certain amount of culture medium, metabolites, and / or other components produced during the cultivation process. The term "culture" also includes passaged cultures obtained by subculturing microorganisms; these can be cultures of a single generation or mixtures of several generations.

[0012] In the above text, the substances in the culture include metabolites.

[0013] The metabolites can be obtained from the fermentation broth of strain QY5. The metabolites of strain QY5 can be either sterile metabolites of strain QY5 or microbial metabolites of strain QY5. Specifically, sterile metabolites of strain QY5 (sterile fermentation filtrate) can be prepared by culturing strain QY5 in a liquid culture medium and filtering to remove strain QY5 from the liquid culture (fermentation broth). Microbial metabolites of strain QY5 can be prepared by culturing strain QY5 in a liquid fermentation medium and collecting the fermentation broth (containing strain QY5 and substances secreted into the liquid culture medium); this fermentation broth is the microbial metabolite of strain QY5.

[0014] Thirdly, the present invention provides a composition containing the QY5 strain or a culture thereof as described in the first aspect.

[0015] The composition described above may also include other substances for degrading polypropylene (such as chemicals) or other strains for degrading polypropylene (such as biocontrol agents composed of other strains that degrade polypropylene).

[0016] Fourthly, the present invention provides the QY5 strain described in the first aspect, the culture described in the second aspect, or the composition described in the third aspect to have at least one of the following functions: 1) Degradation of polypropylene; 2) Preparation of degradable polypropylene products.

[0017] In the applications described above, the product may be a microbial agent or a microecological preparation.

[0018] Fifthly, the present invention provides a product having the function of degrading polypropylene, wherein the active ingredient comprises the QY5 strain described in the first aspect, the culture described in the second aspect, or the composition described in the third aspect.

[0019] The products mentioned above are microbial agents or microecological preparations.

[0020] The above-mentioned microbial agents may also contain other biological or non-biological components. Other active ingredients of the above-mentioned microbial agents can be determined by those skilled in the art based on the effects of the microbial agents.

[0021] The aforementioned microbial agent may further include a carrier. The carrier may be a solid carrier or a liquid carrier.

[0022] The solid carrier may be a mineral material or a biological material; the mineral material may be at least one of peat moss, clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth; the biological material may be at least one of various crop straws, pine shells, rice straw, peanut shells, corn flour, soybean flour, starch, peat moss, and animal excrement; the liquid carrier may be water.

[0023] In the microbial agent, the metabolites of strain QY5 may exist in the form of cultured live cells, fermentation broth of live cells, filtrate of cell culture, or a mixture of cells and filtrate. The microbial agent can be in various formulations, such as liquid, emulsion, suspension, powder, granules, wettable powder, or water-dispersible granules.

[0024] Depending on the requirements, surfactants (such as Tween 20, Tween 80, etc.), binders, stabilizers (such as antioxidants), pH adjusters, etc. may also be added to the bacterial agent.

[0025] In a sixth aspect, the present invention provides a method for degrading polypropylene, comprising the following steps: contacting a sample containing polypropylene with the QY5 strain described in the first aspect, the culture described in the second aspect, the composition described in the third aspect, or the product described in the fifth aspect to achieve degradation of polypropylene.

[0026] In the method described above, the polypropylene exists in the form of polypropylene plastic powder or polypropylene plastic granules.

[0027] The aforementioned plastics may be aged plastics.

[0028] In the method described above, the contact can be co-culture.

[0029] In the above text, the degradation is described as biodegradation.

[0030] In the above text, the sample containing polypropylene may exist in the form of polypropylene plastic powder, polypropylene plastic granules or polypropylene film, or it may be soil from a plastic-contaminated site, sewage sample, or plastic waste in the environment soil, etc.

[0031] The polypropylene mentioned above can exist in the form of polypropylene plastic powder, polypropylene plastic granules, or polypropylene film. It can exist in different water bodies (such as sewage, lakes, or oceans), soil (such as polluted soil), and plastics of various forms and specifications.

[0032] The present invention has the following beneficial effects: 1) This invention enriches and purifies the Sphingomonas spp. QY5 strain in contaminated soil; 2) The *Sphingomonas* strain QY5 of this invention can grow using polypropylene as the sole carbon source and exhibits tolerance to polypropylene concentrations up to 1000 mg / L. Further analysis revealed that after treatment with this strain, the infrared signature functional groups and differential scanning calorimetry (DSC) characteristics of polypropylene were significantly weakened, and the thermodynamic properties of the material also changed. These physicochemical changes may indicate that strain QY5 possesses potential bioactivity for degrading and transforming polypropylene plastics. It can be used to degrade polypropylene plastics in various environments, enriching the polypropylene-degrading bacterial strain library.

[0033] 3) The Sphingosporobacter spp. QY5 strain of the present invention can be used to prepare polypropylene degrading agents, which can reduce microplastic residues and environmental load, and has broad application potential and market value.

[0034] Preservation Instructions Strain name: QY5 Latin name: Sphingobacterium sp. Preservation Institution: China Center for Type Culture Collection Address: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province Deposit date: January 8, 2026 Collection Center Registration Number: CCTCC NO: M 2026053 Classified and named as: Sphingosine Bacillus Sphingobacterium sp. Attached Figure Description Figure 1 The growth of the isolated and purified *Sphingosporobacter* strain QY5 at different time points on purified culture plates supplemented with polypropylene.

[0035] Figure 2 The colony counts of Sphingosporobacter spp. QY5 at different growth times on polypropylene degradation culture plates.

[0036] Figure 3 The infrared spectra of the control and the treatment with added Sphingosporobacter spp. QY5 strain in Example 2 are shown.

[0037] Figure 4 The images show the differential scanning calorimetry (DSC) spectra of the control and the treatment with Sphingosporobacter spp. QY5 in Example 2. Detailed Implementation

[0038] The present invention will now be described in further detail with reference to specific embodiments. The given embodiments are merely illustrative of the invention and not intended to limit its scope. The embodiments provided below can serve as a guide for further improvements by those skilled in the art and do not constitute a limitation on the invention in any way.

[0039] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials and reagents used in the following examples are commercially available.

[0040] Unless otherwise specified, the quantitative experiments in the following examples are all repeated three times, and the results are averaged.

[0041] The reagents and materials used in the following examples are as follows: KH₂PO₄, K₂HPO₄·3H₂O, MgSO₄, NaCl, CaCl₂, Na₂SeO₃·5H₂O, Na₂WO₄·2H₂O, EDTA, FeSO₄·7H₂O, ZnSO₄·7H₂O, MnCl₂·4H₂O, H₃BO₃, CoCl₂·6H₂O, CuCl₂·2H₂O, NiCl₂·6H₂O, NaMoO₄·2H₂O, etc. All culture medium formulations and reagents were of analytical grade. The above reagents, along with BG11 culture medium and agar, were purchased from Sinopharm Group, Qingdao Haibo Biotechnology, Maclean's, Aladdin, Sigma, etc. The microplastics were polypropylene powder, purchased from Shanghai Boer Chemical Reagent Co., Ltd., Lot P234137.

[0042] The instruments in the following embodiments are as follows: Routine culture equipment includes a high-temperature autoclave, a clean bench, a shaker, and a constant-temperature incubator. A 395 nm wavelength LED ultraviolet lamp is used to irradiate the aged microplastics. Testing instruments include an infrared spectrometer-microscope (Thermoelectric (Shanghai) Instruments Co., Ltd., Nicolet 6700FTIR) and a differential scanning calorimeter (TA INSTRUMENTS, Q5000IR).

[0043] The culture medium formulation and preparation method are as follows in the examples below: Liquid plastic-degrading bacteria enrichment medium (BG11 medium) was prepared using commercially available BG11 medium powder. Solid purification medium with plastic mother liquor as the sole carbon source and solid degradation medium with microplastic powder as the sole carbon source were also prepared.

[0044] When preparing BG11 medium, the medium powder is thoroughly mixed and dissolved in ultrapure water, sterilized by high temperature and high pressure, and then used directly after cooling.

[0045] The purification medium was prepared as follows: Weigh the following solutes: 1 g KH₂PO₄, 3 g K₂HPO₄·3H₂O, 0.2 g MgSO₄, 1 g NaCl, 0.01 g CaCl₂, 0.006 g Na₂SeO₃·5H₂O, 0.008 g Na₂WO₄·2H₂O, 0.0005 g EDTA, 0.0002 g FeSO₄·7H₂O, 0.00001 g ZnSO₄·7H₂O, 0.000003 g MnCl₂·4H₂O, 0.00003 g H₃BO₃, 0.00002 g CoCl₂·6H₂O, 0.000001 g CuCl₂·2H₂O, 0.000002 g NiCl₂·6H₂O, 0.000003 g NaMoO₄·2H₂O, 15 g Add agar, mix thoroughly, and bring the volume to 1 L with ultrapure water. After high temperature and high pressure sterilization, wait for the temperature to cool to about 60 °C, add polypropylene stock solution (polypropylene aqueous solution) to make the polypropylene concentration 1000 mg / L, mix well, pour into the wells of a microplate, cool and solidify for later use, and obtain a purification plate containing 1000 mg / L polypropylene.

[0046] The degradation medium was prepared as follows: Weigh the following solutes: 1 g KH₂PO₄, 3 g K₂HPO₄·3H₂O, 0.2 g MgSO₄, 1 g NaCl, 0.01 g CaCl₂, 0.006 g Na₂SeO₃·5H₂O, 0.008 g Na₂WO₄·2H₂O, 0.0005 g EDTA, 0.0002 g FeSO₄·7H₂O, 0.00001 g ZnSO₄·7H₂O, 0.000003 g MnCl₂·4H₂O, 0.00003 g H₃BO₃, 0.00002 g CoCl₂·6H₂O, 0.000001 g CuCl₂·2H₂O, 0.000002 g NiCl₂·6H₂O, 0.000003 g NaMoO₄·2H₂O, 15 g Add agar, mix thoroughly, and then bring the volume to 1 L with ultrapure water. After high-temperature and high-pressure sterilization, add polypropylene powder to make the polypropylene concentration 1000 mg / L, mix well, cool and solidify for later use, and obtain degradation plates containing 1000 mg / L polypropylene.

[0047] Example 1: Screening and Isolation of High-Throughput Plastic-Degrading Bacteria and Polypropylene-Degrading Bacteria This invention employs a high-throughput enrichment method for plastic-degrading bacteria. First, mineral water is added to contaminated soil and shaken for 24 hours to activate the bacteria, creating a bacterial suspension. Then, the suspension is added to a liquid plastic-degrading bacteria enrichment medium (BG11 medium) to activate the bacteria, followed by the addition of pretreated polypropylene solution and shaking for 48 hours. Finally, the polypropylene-degrading bacteria are further isolated and purified in a solid purification medium using polypropylene as the sole carbon source. Specific processing details are as follows: I. Reagents and culture media required for the isolation and identification of polypropylene-degrading bacteria Polypropylene aging pretreatment: Polypropylene powder was added to 100 mL of water to prepare a 10 g / L polypropylene mother liquor; under an LED lamp (395 nm wavelength) simulating natural ultraviolet irradiation, the beaker was placed in a magnetic stirrer and stirred continuously to simulate environmental aging. The mixture was incubated at 25 °C for 7 days to obtain the aged polypropylene mother liquor.

[0048] The purified culture medium powder was thoroughly mixed and dissolved in ultrapure water. After high temperature and high pressure sterilization, the temperature was cooled to about 60 °C and aged polypropylene stock solution was added and mixed (to make the polypropylene concentration 1000 mg / L). The mixture was then poured into the wells of a microplate and cooled to solidify for later use, thus obtaining the purified culture medium.

[0049] II. Isolation and Identification of Polypropylene Degrading Strains QY5 1. Isolation of strain QY5 This invention employs a perforated plate plastic enrichment method to separate plastic-degrading bacteria: 1) Isolation of soil bacteria and preparation of bacterial suspension Add 10 g of soil from a plastic-contaminated site (isolated from a garbage dump in Inner Mongolia) and 100 mL of water to a conical flask, and culture on a shaker at 30 ℃ and 180 rpm / min for 24 h to prepare a soil bacterial suspension. 2) Activation Add 100 μL of the soil bacterial suspension obtained in step 1) above and 800 μL of BG11 medium to each well of a 48-well plate and shake to activate for 24 h; 3) Domestication Add 100 μL of aged polypropylene stock solution (1000 mg / L) to each well after activation in step 2) above, and culture in a shaker at 30 °C and 80 rpm / min for 48 h to obtain domesticated degrading bacteria; 4) Separation According to the formula of the purified culture medium, the solid culture medium prepared without the addition of polypropylene stock solution is recorded as the carbon-nitrogen-free control plate.

[0050] The domesticated degrading bacteria obtained in step 3) were streaked onto a purification plate (purification medium) containing 1000 mg / L polypropylene and a carbon-nitrogen-free control plate. The plates were incubated at 30 °C for 20 days, and the growth of bacterial colonies was observed and photographed.

[0051] Bacteria that can form colonies on purification plates containing 1000 mg / L polypropylene but cannot grow on carbon-nitrogen-free control plates were selected and isolated and purified as single colonies. These were then known as polypropylene-degrading pure bacteria and named strain QY5.

[0052] Figure 1 The results show the growth of strain QY5 on purification plates containing 1000 mg / L polypropylene for different time periods. It can be seen that colonies can grow on purification plates containing 1000 mg / L polypropylene after 9 days of cultivation. The colonies of this strain typically have neat or slightly wavy edges, a smooth, moist, and glossy surface, are slightly to moderately raised, have a viscous and soft texture, are uniform in shape, and are regularly distributed. On the purification medium, they are relatively light in color and semi-transparent.

[0053] 2. Molecular identification of strain QY5 Genomic DNA was extracted from strain QY5 and used as a template. PCR amplification was performed using 27F: AGAGTTTGATCMTGGCTCAG and 1492R: GGTTACCTTGTTACGACTT as primers to obtain the PCR amplification product.

[0054] The PCR amplification products were sent for 16S rDNA sequencing, and the 16S rDNA sequence is shown in SEQ ID NO:1. After comparison, strain QY5 was identified as belonging to the genus *Sphingomonas*. Sphingobacterium sp.).

[0055] Sphingosporobacter spp. Sphingobacterium strain QY5 was deposited on January 8, 2026, at the China Center for Type Culture Collection (address: No. 299, Bayi Road, Wuchang District, Wuhan, Hubei Province), with accession number CCTCC NO: M2026053. Hereinafter referred to as strain QY5.

[0056] Example 2: Growth of Sphingosporobacter spp. strain QY5 in plastic degradation medium. The growth of Sphingosporobacter spp. QY5 in polypropylene was verified in a degradation medium with added plastic powder as the sole carbon source.

[0057] According to the formulation of the degradation medium, the solid medium prepared without the addition of polypropylene powder is recorded as a degradation plate of 0 mg / L polypropylene.

[0058] Single colonies of the *Sphingosporobacter* strain QY5 obtained in Example 1 were streaked onto degradation plates (degradation medium) containing 1000 mg / L polypropylene and 0 mg / L polypropylene. The plates were then incubated in a constant temperature shaker at 30 ℃ for 10 days. The colony growth was observed and recorded by taking pictures to determine the range of polypropylene concentrations in which colonies could grow.

[0059] The growth results of Sphingomyelin Bacillus strain QY5 are as follows: Figure 2 As shown, "bacterium + carbon-nitrogen-free medium" represents a degradation plate containing 0 mg / L polypropylene inoculated with strain QY5, and "bacterium + carbon-nitrogen-free medium + PP" represents a degradation plate containing 1000 mg / L polypropylene inoculated with strain QY5. The results show that *Sphingosporobacter* strain QY5 cannot grow in degradation plates containing 0 mg / L polypropylene (no colony growth), but colonies are visible to the naked eye after 6 days of culture on degradation plates containing 1000 mg / L polypropylene. This indicates that *Sphingosporobacter* strain QY5 can grow with polypropylene as the sole carbon source and can tolerate 1000 mg / L polypropylene.

[0060] Example 3: Identification of polypropylene degradation by Sphingomyelin Bacillus spp. QY5 strain I. Infrared Spectroscopic Identification The degradation system used in this embodiment is the same as that in Example 2. The degradation system was identified by infrared spectroscopy, as follows: Add QY5 group: Single colony of Sphingosporobacter spp. strain QY5 obtained in Example 1 was streaked on a degradation plate containing 1000 mg / L polypropylene and incubated in a constant temperature shaker at 30 ℃ for 10 days to obtain the culture product.

[0061] Control group: Degradation plates containing 1000 mg / L polypropylene were incubated statically in a shaker at 30 ℃ for 10 days.

[0062] The culture products from each group were freeze-dried and then subjected to infrared spectroscopy.

[0063] The results are as follows Figure 3 As shown in the figure, the upper figure represents the treatment group with added QY5 (denoted as YCG-PPB4-0906A in the figure), and the lower figure represents the control group (denoted as YCG-PPCK-0906A in the figure). It can be seen that, compared with the control sample, the QY5-added group showed better results at 3200–3600 cm⁻¹. -¹ A significant broad O–H peak appears, at 1740–1715 cm⁻¹. -¹ A C=O carbonyl band appears, and it is located between 1167 and 1000 cm⁻¹. -¹ C–O related absorptions were observed, with values ​​ranging from 2955 to 2838 cm⁻¹. -¹The relative decrease in C–H content of hydrocarbons is 998 / 973 / 841 cm⁻¹. -¹ The crystallographic fingerprint bands changed. These characteristics collectively indicate that polypropylene underwent oxidation, introducing oxygen-containing functional groups accompanied by chain cleavage and structural rearrangement, suggesting that polypropylene underwent a biodegradation process under the action of strain QY5.

[0064] II. Differential Scanning Calorimetry Identification Add QY5 group: Single colony of Sphingosporobacter spp. strain QY5 obtained in Example 1 was streaked on a degradation plate containing 1000 mg / L polypropylene and incubated in a constant temperature shaker at 30 ℃ for 10 days to obtain the culture product.

[0065] Control group: Degradation plates containing 1000 mg / L polypropylene were incubated statically in a shaker at 30 ℃ for 10 days.

[0066] The culture products from each group were identified by differential scanning calorimetry (DSC) with temperature ranges from 20 to 180°C. A heating rate of 10 °C / min was used for the first and second cooling and heating cycles in nitrogen.

[0067] The results are as follows Figure 4 As shown in Figure A, the treatment group with added QY5 is represented by Figure B, while the control group is represented by Figure B. It can be seen that the peak changes during heating and cooling scans reveal a significant decrease in melting point (Tm) of approximately 5-10°C and a reduction in enthalpy of fusion (ΔHf). This indicates that the biodegradation process effectively reduced the polymer's molecular weight and disrupted its original crystal structure, resulting in broken molecular chains and imperfect crystals. Furthermore, a new exothermic peak appeared in the QY5-added sample at a high temperature (approximately 200-250°C), indicating that the polymer underwent oxidation, a reaction typically initiated by microorganisms during metabolism. Therefore, the decrease in melting point, change in crystallinity, and exothermic oxidation peak shown in the DSC data collectively demonstrate that a biodegradation process characterized by chain breakage and oxidation occurred under the action of *Sphingomyelinum* strain QY5, indicating that *Sphingomyelinum* strain QY5 has a certain capacity to utilize plastics.

[0068] In summary, this demonstrates that *Sphingosporobacter* spp. QY5 has the ability to degrade polypropylene and can be used for biodegradable plastics.

[0069] The present invention has been described in detail above. For those skilled in the art, the invention can be practiced in a wide range of ways with equivalent parameters, concentrations, and conditions without departing from its spirit and scope, and without requiring unnecessary experiments. Although specific embodiments have been given, it should be understood that further modifications can be made to the invention. In summary, according to the principles of the invention, this application is intended to include any changes, uses, or improvements to the invention, including changes made using conventional techniques known in the art that depart from the scope disclosed herein. Some of the essential features can be applied within the scope of the following appended claims.

Claims

1. Sphingosine bacillus ( Sphingobacterium The strain QY5 (sp.) has the accession number CCTCC NO: M2026053.

2. The culture of the QY5 strain according to claim 1 is a substance obtained by culturing the QY5 strain according to claim 1 in a microbial culture medium.

3. A composition containing the QY5 strain of claim 1 or the culture of claim 2.

4. The QY5 strain of claim 1, the culture of claim 2, or the composition of claim 3 has at least one of the following functions: 1) Degradation of polypropylene; 2) Preparation of degradable polypropylene products.

5. The application according to claim 4, characterized in that: The product is a microbial agent or a microecological preparation.

6. A product having the function of degrading polypropylene, wherein the active ingredient comprises the QY5 strain of claim 1, the culture of claim 2, or the composition of claim 3.

7. The product according to claim 6, characterized in that: The product is a microbial agent or a microecological preparation.

8. A method for degrading polypropylene, comprising the following steps: contacting a sample containing polypropylene with the QY5 strain of claim 1, the culture of claim 2, the composition of claim 3, or the product of claim 6 or 7 to achieve degradation of polypropylene.

9. The method according to claim 8, characterized in that: The polypropylene exists in the form of polypropylene plastic powder or polypropylene plastic granules.

10. The application according to claim 4 or 5, or the product according to claim 6 or 7, or the method according to claim 8 or 9, characterized in that: The degradation is biodegradation.

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