Hair dyeing composition based on hydrogen peroxide and preparation method thereof

Abstract

The invention relates to the technical field of hair dyes, in particular to a hair dyeing composition based on hydrogen peroxide and a preparation method thereof.The hair dyeing composition is composed of a first component and a second component, in the hair dyeing process, hair cuticles on the outermost layer of hair are opened firstly, and then the hair cuticles on the outermost layer of the hair are opened; the first component and the second component enter the hair through small holes opened by hair cuticles, the hydrogen peroxide in the second component bleaches pigments in the hair, and then the hydrogen peroxide and the 5, 6-dihydroxyindole entering the first component are subjected to an oxidation reaction, and dyeing is completed; wherein an anti-damage composition in the second component is obtained by mixing sodium hyaluronate, a Japanese callicarpa fruit fermentation extract, a phaeodactylum tricornutum extract and a microbial composition, and the microbial composition subjected to thermal inactivation treatment has anti-damage and repairing effects on hair, is high in stability, is not liable to lose efficacy due to the influence of a hydrogen peroxide oxidizing agent, and has a good application prospect. Wide application prospects are realized.

Description

Technical Field

[0001] This invention relates to the field of hair dye technology, and more particularly to a hydrogen peroxide-based hair dye composition and its preparation method. Background Technology

[0002] Hair dyeing is a beauty process, whether for the elderly covering gray hair or for young people seeking a trendy look. Hair dye products are a crucial category within the cosmetics and hair care industry. The quality of hair dyes directly impacts consumer safety, and the research and development of safe and effective new hair dyes is booming in the cosmetics industry.

[0003] Hair dye is a special-purpose cosmetic. Based on the source of the dye, hair dyes are classified into three categories: plant-based dyes, metal salt dyes, and synthetic organic dyes. Based on the dyeing principle and fastness, they are classified into temporary hair dyes, semi-permanent hair dyes, and permanent (oxidative) hair dyes. Permanent (oxidative) hair dyes typically use a mixture of dyeing agents and oxidizing agents. The dye formulation contains dye intermediates and coupling agents. These intermediates and coupling agents penetrate the scalp and hair, undergoing four chemical reactions under the action of the oxidizing agent: neutralization, redox, polymerization, and condensation. The small dye molecules polymerize into large molecules that encapsulate and fix in the hair cortex and medulla, resulting in relatively long-lasting coloring. Therefore, poor-quality hair dyes can be highly irritating to the hair and scalp.

[0004] Hydrogen peroxide's oxidizing properties can remove the natural melanin pigment inside the hair and help artificial pigments oxidize and combine in the cortex to produce new colors. During hair dyeing, the alkalinity of the dye causes the hair cuticles to open, while hydrogen peroxide helps them close, making it a crucial factor affecting hair quality and color presentation. However, hydrogen peroxide is a strong oxidizing agent, and improper use can severely damage hair, causing it to become dry, dull, and lacking elasticity after dyeing.

[0005] Therefore, based on the relevant technologies mentioned above, there is an urgent need to develop a hair dye composition based on hydrogen peroxide and its preparation method. Summary of the Invention

[0006] In view of this, the purpose of this invention is to provide a hydrogen peroxide-based hair dyeing composition and its preparation method, so as to solve the problem that hydrogen peroxide hair dyeing compositions in the prior art are prone to causing hair damage.

[0007] To achieve the above objectives, the present invention provides a hydrogen peroxide-based hair dye composition and a method for preparing the same.

[0008] A hydrogen peroxide-based hair dye composition, the hydrogen peroxide-based hair dye composition comprising a first component and a second component; The mass ratio of the first component to the second component is 1:1.2-1.3; The first component comprises the following parts by weight of raw materials: 5,6-Dihydroxyindole 6-9 parts; C16-18 alcohol 9-17.5 parts; cetyl PEG 3-5 parts; olive oil 15-20 parts; hexadecyltrimethylammonium chloride 1-3 parts; cocamidobetaine 2-3 parts; Tween 80 1-2 parts; cysteine ​​3.3-6.8 parts; threonine 1-2 parts; xanthan gum 1-2 parts; hydrolyzed protein 2-3 parts; water-soluble lanolin 2-3 parts; deionized water 88-97 parts; 10% citric acid aqueous solution; The second component comprises the following parts by weight of raw materials: Hydrogen peroxide 1.5-3.2 parts; xanthan gum 1-2 parts; glycerin 5-7 parts; oleyl alcohol 5-7 parts; hydrogen peroxide stabilizer 0.01-0.03 parts; anti-damage composition 5.5-7.8 parts; deionized water 93-110 parts; The anti-damage composition is obtained by mixing sodium hyaluronate, fermented extract of Japanese purple palm fruit, extract of brown finger algae, and a microbial composition. The microbial composition was prepared from Lactobacillus plantarum L30 and Bifidobacterium 070310.

[0009] Preferably, the mass ratio of sodium hyaluronate, Japanese purple palm fruit fermentation extract, brown finger algae extract and microbial composition is 1-1.5:7.2-9.6:3-6:3.5-4.8.

[0010] Preferably, the preparation method of the microbial composition is as follows: Lactobacillus plantarum L30 and Bifidobacterium 070310 were activated in sterile MRS culture medium at 37℃ for 22-24 h, centrifuged at 4000-5000 rpm for 15-20 min, washed three times with sterile water, and resuspended in phosphate buffer solution to a concentration of 1×10⁻⁶. 10 -3.5×10 10 The concentration of CFU / mL was then subjected to heat inactivation to obtain the microbial composition.

[0011] Preferably, the MRS culture medium formula is as follows: 10g peptone, 10g beef extract, 5g yeast extract, 20g glucose, 1mL Tween-80, 2g diammonium hydrogen citrate, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 0.58g magnesium sulfate heptahydrate, 0.25g manganese sulfate tetrahydrate, 1000mL distilled water, and pH 6.8.

[0012] Preferably, the temperature during the heat inactivation treatment is 90-100℃ and the time is 10 minutes.

[0013] Preferably, the preparation method of the *Phaeodactylum tricornutum* extract is as follows: Add *Phaeodactylum tricornutum* to a 95% ethanol aqueous solution, add 30% sodium hydroxide solution to adjust the pH of the system to 13, and reflux extract at 75-80℃ for 20-30 min under a nitrogen atmosphere. Cool to 18-20℃, filter, add deionized water and stir to form an aqueous-ethanol phase. Perform two-phase extraction, elute the aqueous-ethanol phase with n-heptane, collect the lower layer to obtain fatty acid salts, extract twice, acidify the lower layer to pH 2.5 with sulfuric acid, elute with n-heptane, and rotary evaporate to obtain *Phaeodactylum tricornutum* extract.

[0014] Preferably, the ratio of the amount of *Phaeodactylum tricornutum*, 95% ethanol aqueous solution, and deionized water is 10-20g:280-500mL:1500mL.

[0015] Preferably, the fermented extract of Japanese purple pearl fruit is obtained by enzymatic hydrolysis of Japanese purple pearl fruit with cellulase, fermentation with Lactobacillus plantarum and active dry yeast, and subsequent ripening treatment. The number-average molecular weight of the sodium hyaluronate is 10-40 kDa.

[0016] Preferably, the hydrogen peroxide stabilizer is at least one of phenacetin, disodium hydrogen phosphate, and disodium dihydrogen pyrophosphate.

[0017] A method for preparing a hydrogen peroxide-based hair dye composition includes the following steps: Step S1. Mix C16-18 alcohol, cetyl PEG, cocamidocyanate, olive oil and hexadecyltrimethylammonium chloride and heat to 70-80℃ to obtain the oil phase; mix cysteine, Tween 80, threonine, xanthan gum and deionized water at 70-75℃ until homogeneous, then cool to 35-40℃ and add hydrolyzed protein and water-soluble lanolin to obtain the aqueous phase; Step S2. Add the oil phase to the aqueous phase and stir homogenously at 40-50℃ and keep warm for 10-20 min. Add 10% citric acid aqueous solution to adjust the pH of the system to 4, then add 5,6-dihydroxyindole and mix well to obtain the first component. Step B3. Mix glycerin, oleyl alcohol, xanthan gum, hydrogen peroxide stabilizer, anti-damage composition and deionized water at 60-70℃ until homogeneous. After the system cools down to 30-35℃, add hydrogen peroxide to obtain the second component, which is the hydrogen peroxide-based hair dye composition.

[0018] The beneficial effects of this invention are: This invention provides a hydrogen peroxide-based hair dyeing composition and its preparation method. The hair dyeing composition consists of a first component and a second component. During the dyeing process, the outermost hair cuticles open first, and the first and second components enter the hair through the pores opened by the cuticles. The hydrogen peroxide in the second component bleaches the pigment inside the hair and then reacts with the 5,6-dihydroxyindole in the first component, which enters at the same time, to complete the dyeing process. The hydrogen peroxide stabilizer in the second component effectively prevents the decomposition of hydrogen peroxide and improves the storage stability of the hair dyeing composition. The anti-damage composition is obtained by mixing sodium hyaluronate, fermented extract of Japanese purple palm fruit, extract of brown finger algae, and a microbial composition. The microbial composition, after heat inactivation treatment, has anti-damage and repair effects on hair, while also exhibiting high stability and being less susceptible to failure due to the influence of hydrogen peroxide and hydrogen peroxide. The hydrogen peroxide-based hair dye composition prepared in this invention not only meets the technical requirements of special cosmetics such as hair dyes, but also possesses excellent formulation compatibility, gentleness, and efficacy extensibility. It can be directly adapted to various common cosmetic categories, such as shampoo coloring, conditioner, hair conditioning agents, hair care products, hair masks, leave-in conditioners, scalp serums, hair oils, styling gels, scalp care gels, hair waxes, and 2-in-1 shampoos and conditioners, etc. This enables the cross-scenario reuse of active ingredients in both special and common cosmetic fields, and has broad application prospects compared to existing technologies. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments.

[0020] Example 1: A method for preparing a hydrogen peroxide-based hair dye composition, comprising the following steps: S1. Weigh 10g peptone, 10g beef extract, 5g yeast extract, 20g glucose, 2g diammonium citrate, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 0.58g magnesium sulfate heptahydrate and 0.25g manganese sulfate tetrahydrate, add 1000mL distilled water and 1mL Tween-80 and stir until completely dissolved, adjust pH to 6.8, filter and sterilize at 121℃ for 15min to obtain MRS culture medium; S2. Activate *Lactobacillus plantarum* L30 and *Bifidobacterium* 070310 in sterile MRS culture medium at 37℃ for 22 h, centrifuge at 4000 rpm for 15 min, wash three times with sterile water, and resuspend in phosphate buffer solution to a concentration of 1×10⁻⁶. 10 The concentration of CFU / mL was further reduced by heat inactivation at 90°C for 10 min to obtain the microbial composition. S3. Add 10g of *Phaeodactylum tricornutum* to 280mL of 95% ethanol aqueous solution, add 30% sodium hydroxide solution to adjust the pH of the system to 13, reflux extract at 75℃ for 20min under nitrogen atmosphere, cool to 18℃, filter, add 1500ml of deionized water and stir to form water-ethanol phase, extract by two-phase extraction, elute the water-ethanol phase with n-heptane, collect the lower layer to obtain fatty acid salt, extract twice, acidify the lower layer to pH 2.5 with sulfuric acid, elute with n-heptane and rotary evaporate to obtain *Phaeodactylum tricornutum* extract; S4. The Japanese purple fruit was hydrolyzed by cellulase, fermented with Lactobacillus plantarum and active dry yeast, and then matured after fermentation to obtain a fermented extract of Japanese purple fruit. 1g of sodium hyaluronate, 7.2g of fermented extract of Japanese purple fruit, 3g of brown finger algae extract and 3.5g of microbial composition were mixed to obtain an anti-damage composition. S5. Mix 9g C16-18 alcohol, 3g cetyl PEG, 2g cocamidocyanate, 15g olive oil and 1g hexadecyltrimethylammonium chloride and heat to 70℃ to obtain the oil phase; mix 3.3g cysteine, 1g Tween 80, 1g threonine, 1g xanthan gum and 88g deionized water at 70℃ until homogeneous, then cool to 35℃ and add 2g hydrolyzed protein and 2g water-soluble lanolin to obtain the aqueous phase; S6. Add the oil phase to the aqueous phase and stir homogenously at 40℃ and keep warm for 10 min. Add a 10% citric acid aqueous solution to adjust the pH of the system to 4, then add 6g of 5,6-dihydroxyindole and mix well to obtain the first component. S7. Mix 5g glycerin, 5g oleyl alcohol, 1g xanthan gum, 0.01g hydrogen peroxide stabilizer, 5.5g anti-damage composition and 93g deionized water at 60℃ until homogeneous. After the system cools down to 30℃, add 1.5g hydrogen peroxide to obtain the second component, which is the hydrogen peroxide-based hair dye composition.

[0021] Example 2: A method for preparing a hydrogen peroxide-based hair dye composition, comprising the following steps: S1. Weigh 10g peptone, 10g beef extract, 5g yeast extract, 20g glucose, 2g diammonium citrate, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 0.58g magnesium sulfate heptahydrate and 0.25g manganese sulfate tetrahydrate, add 1000mL distilled water and 1mL Tween-80 and stir until completely dissolved, adjust pH to 6.8, filter and sterilize at 121℃ for 15min to obtain MRS culture medium; S2. Lactobacillus plantarum L30 and Bifidobacterium 070310 were activated in sterile MRS culture medium at 37℃ for 23 h, centrifuged at 4000 rpm for 15 min, washed three times with sterile water, and resuspended in phosphate buffer solution to a concentration of 1.5 × 10⁻⁶. 10 The concentration of CFU / mL was further reduced by heat inactivation at 93°C for 10 min to obtain the microbial composition. S3. Add 13g of *Phaeodactylum tricornutum* to 330mL of 95% ethanol aqueous solution, add 30% sodium hydroxide solution to adjust the pH of the system to 13, reflux extract at 75℃ for 23min under nitrogen atmosphere, cool to 18℃, filter, add 1500ml of deionized water and stir to form water-ethanol phase, extract by two-phase extraction, elute the water-ethanol phase with n-heptane, collect the lower layer to obtain fatty acid salt, extract twice, acidify the lower layer to pH 2.5 with sulfuric acid, elute with n-heptane and rotary evaporate to obtain *Phaeodactylum tricornutum* extract; S4. The Japanese purple fruit was hydrolyzed by cellulase, fermented with Lactobacillus plantarum and active dry yeast, and then matured after fermentation to obtain a fermented extract of Japanese purple fruit. 1.1g sodium hyaluronate, 7.7g fermented extract of Japanese purple fruit, 3.5g extract of Brownia triangularis and 3.8g microbial composition were mixed to obtain an anti-damage composition. S5. Mix 10g C16-18 alcohol, 3.4g cetyl PEG, 2.3g cocamidopiclinide, 16g olive oil, and 1.3g hexadecyltrimethylammonium chloride and heat to 73°C to obtain the oil phase; mix 3.8g cysteine, 1.3g Tween 80, 1.3g threonine, 1.3g xanthan gum, and 90g deionized water at 72°C until homogeneous, then cool to 35°C and add 2.3g hydrolyzed protein and 2.3g water-soluble lanolin to obtain the aqueous phase; S6. Add the oil phase to the aqueous phase and stir homogenously at 43℃ for 13 min. Add 10% citric acid aqueous solution to adjust the pH of the system to 4. Then add 6.5g of 5,6-dihydroxyindole and mix well to obtain the first component. S7. Mix 5.4g glycerin, 5.4g oleyl alcohol, 1.2g xanthan gum, 0.015g hydrogen peroxide stabilizer, 6g anti-damage composition and 98g deionized water at 64℃ until homogeneous. After the system cools down to 32℃, add 1.8g hydrogen peroxide to obtain the second component, which is the hydrogen peroxide-based hair dye composition.

[0022] Example 3: A method for preparing a hydrogen peroxide-based hair dye composition, comprising the following steps: S1. Weigh 10g peptone, 10g beef extract, 5g yeast extract, 20g glucose, 2g diammonium citrate, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 0.58g magnesium sulfate heptahydrate and 0.25g manganese sulfate tetrahydrate, add 1000mL distilled water and 1mL Tween-80 and stir until completely dissolved, adjust pH to 6.8, filter and sterilize at 121℃ for 15min to obtain MRS culture medium; S2. Lactobacillus plantarum L30 and Bifidobacterium 070310 were activated in sterile MRS culture medium at 37℃ for 23 h, centrifuged at 4500 rpm for 18 min, washed three times with sterile water, and resuspended in phosphate buffer solution to a concentration of 2 × 10⁻⁶. 10 The concentration of CFU / mL was further reduced by heat inactivation at 95°C for 10 min to obtain the microbial composition. S3. Add 15g of *Phaeodactylum tricornutum* to 400mL of 95% ethanol aqueous solution, add 30% sodium hydroxide solution to adjust the pH of the system to 13, reflux extract at 78℃ for 25min under nitrogen atmosphere, cool to 18℃, filter, add 1500ml of deionized water and stir to form water-ethanol phase, extract by two-phase extraction, elute the water-ethanol phase with n-heptane, collect the lower layer to obtain fatty acid salt, extract twice, acidify the lower layer to pH 2.5 with sulfuric acid, elute with n-heptane and rotary evaporate to obtain *Phaeodactylum tricornutum* extract; S4. The Japanese purple fruit was hydrolyzed by cellulase, fermented with Lactobacillus plantarum and active dry yeast, and then matured after fermentation to obtain a fermented extract of Japanese purple fruit. 1.2g of sodium hyaluronate, 8.3g of fermented extract of Japanese purple fruit, 4g of Brown finger algae extract and 4.1g of microbial composition were mixed to obtain an anti-damage composition. S5. Mix 11.5g C16-18 alcohol, 3.8g cetyl PEG, 2.5g cocamidopiclinide, 17g olive oil and 2g hexadecyltrimethylammonium chloride and heat to 75℃ to obtain the oil phase; mix 4.5g cysteine, 1.5g Tween 80, 1.5g threonine, 1.5g xanthan gum and 92g deionized water at 72℃ until homogeneous, then cool to 38℃ and add 2.5g hydrolyzed protein and 2.5g water-soluble lanolin to obtain the aqueous phase; S6. Add the oil phase to the aqueous phase and stir homogenously at 45°C for 15 min. Add a 10% citric acid aqueous solution to adjust the pH of the system to 4. Then add 7g of 5,6-dihydroxyindole and mix well to obtain the first component. S7. Mix 6g glycerin, 6g oleyl alcohol, 1.6g xanthan gum, 0.02g hydrogen peroxide stabilizer, 6.8g anti-damage composition and 100g deionized water at 66℃ until homogeneous. After the system cools down to 33℃, add 2.5g hydrogen peroxide to obtain the second component, which is the hydrogen peroxide-based hair dye composition.

[0023] Example 4: A method for preparing a hydrogen peroxide-based hair dye composition, comprising the following steps: S1. Weigh 10g peptone, 10g beef extract, 5g yeast extract, 20g glucose, 2g diammonium citrate, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 0.58g magnesium sulfate heptahydrate and 0.25g manganese sulfate tetrahydrate, add 1000mL distilled water and 1mL Tween-80 and stir until completely dissolved, adjust pH to 6.8, filter and sterilize at 121℃ for 15min to obtain MRS culture medium; S2. Activate *Lactobacillus plantarum* L30 and *Bifidobacterium* 070310 in sterile MRS culture medium at 37℃ for 24 h, centrifuge at 5000 rpm for 20 min, wash three times with sterile water, and resuspend in phosphate buffer solution to a concentration of 3 × 10⁻⁶. 10 CFU / mL, then heat-inactivated at 97℃ for 10 min to obtain the microbial composition; S3. Add 17.5g of *Phaeodactylum tricornutum* to 450mL of 95% ethanol aqueous solution, add 30% sodium hydroxide solution to adjust the pH of the system to 13, reflux extract at 80℃ for 28min under nitrogen atmosphere, cool to 20℃, filter, add 1500ml of deionized water and stir to form water-ethanol phase, extract by two-phase extraction, elute the water-ethanol phase with n-heptane, collect the lower layer to obtain fatty acid salt, extract twice, acidify the lower layer to pH 2.5 with sulfuric acid, elute with n-heptane and rotary evaporate to obtain *Phaeodactylum tricornutum* extract; S4. The Japanese purple fruit was hydrolyzed by cellulase, fermented with Lactobacillus plantarum and active dry yeast, and then matured after fermentation to obtain a fermented extract of Japanese purple fruit. 1.3g of sodium hyaluronate, 9g of fermented extract of Japanese purple fruit, 5g of Brown finger algae extract and 4.3g of microbial composition were mixed to obtain an anti-damage composition. S5. Mix 13.5g C16-18 alcohol, 4.5g cetyl PEG, 2.8g cocamidopiclinide, 18g olive oil, and 2.5g hexadecyltrimethylammonium chloride and heat to 78°C to obtain the oil phase; mix 6.1g cysteine, 1.7g Tween 80, 1.8g threonine, 1.8g xanthan gum, and 95g deionized water at 73°C until homogeneous, then cool to 38°C and add 2.7g hydrolyzed protein and 2.7g water-soluble lanolin to obtain the aqueous phase; S6. Add the oil phase to the aqueous phase and stir homogenously at 47°C for 18 min. Add a 10% citric acid aqueous solution to adjust the pH of the system to 4. Then add 8.2 g of 5,6-dihydroxyindole and mix well to obtain the first component. S7. Mix 6.5g glycerin, 6.5g oleyl alcohol, 1.8g xanthan gum, 0.025g hydrogen peroxide stabilizer, 7.2g anti-damage composition and 105g deionized water at 68℃ until homogeneous. After the system cools down to 35℃, add 2.7g hydrogen peroxide to obtain the second component, which is the hydrogen peroxide-based hair dye composition.

[0024] Example 5: A method for preparing a hydrogen peroxide-based hair dye composition, comprising the following steps: S1. Weigh 10g peptone, 10g beef extract, 5g yeast extract, 20g glucose, 2g diammonium citrate, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 0.58g magnesium sulfate heptahydrate and 0.25g manganese sulfate tetrahydrate, add 1000mL distilled water and 1mL Tween-80 and stir until completely dissolved, adjust pH to 6.8, filter and sterilize at 121℃ for 15min to obtain MRS culture medium; S2. Lactobacillus plantarum L30 and Bifidobacterium 070310 were activated in sterile MRS culture medium at 37℃ for 24 h, centrifuged at 5000 rpm for 20 min, washed three times with sterile water, and resuspended in phosphate buffer solution to a concentration of 3.5 × 10⁻⁶. 10 The concentration of CFU / mL was further reduced by heat inactivation at 100°C for 10 min to obtain the microbial composition. S3. Add 20g of *Phaeodactylum tricornutum* to 500mL of 95% ethanol aqueous solution, add 30% sodium hydroxide solution to adjust the pH of the system to 13, reflux extract at 80℃ for 30min under nitrogen atmosphere, cool to 20℃, filter, add 1500ml of deionized water and stir to form water-ethanol phase, extract by two-phase extraction, elute the water-ethanol phase with n-heptane, collect the lower layer to obtain fatty acid salt, extract twice, acidify the lower layer to pH 2.5 with sulfuric acid, elute with n-heptane and rotary evaporate to obtain *Phaeodactylum tricornutum* extract; S4. The Japanese purple fruit was hydrolyzed by cellulase, fermented with Lactobacillus plantarum and active dry yeast, and then matured after fermentation to obtain a fermented extract of Japanese purple fruit. 1.5g sodium hyaluronate, 9.6g fermented extract of Japanese purple fruit, 6g extract of *Phaeodactylum tricornutum*, and 4.8g microbial composition were mixed to obtain an anti-damage composition. S5. Mix 17.5g C16-18 alcohol, 5g cetyl PEG, 3g cocamidocyanate, 20g olive oil and 3g hexadecyltrimethylammonium chloride and heat to 80℃ to obtain the oil phase; mix 6.8g cysteine, 2g Tween 80, 2g threonine, 2g xanthan gum and 97g deionized water at 75℃ until homogeneous, then cool to 40℃ and add 3g hydrolyzed protein and 3g water-soluble lanolin to obtain the aqueous phase; S6. Add the oil phase to the aqueous phase and stir homogenously at 50℃ for 10-20 min. Add a 10% citric acid aqueous solution to adjust the pH of the system to 4. Then add 9g of 5,6-dihydroxyindole and mix well to obtain the first component. S7. Mix 7g glycerin, 7g oleyl alcohol, 2g xanthan gum, 0.03g hydrogen peroxide stabilizer, 7.8g anti-damage composition and 110g deionized water at 70℃ until homogeneous. After the system cools down to 35℃, add 3.2g hydrogen peroxide to obtain the second component, which is the hydrogen peroxide-based hair dye composition.

[0025] Comparative Example 1: Compared with Example 1, this comparative example did not add the anti-damage composition during the preparation of the second component. All other steps and parameters were the same, and will not be repeated here. The final hair dye composition was obtained.

[0026] Comparative Example 2: Compared with Example 1, this comparative example did not add the microbial composition during the preparation of the second component. All other steps and parameters were the same, and will not be repeated here. The final hair dye composition was obtained.

[0027] Comparative Example 3: Compared with Example 1, this comparative example did not add the extract of *Phaeodactylum tricornutum* during the preparation of the second component. All other steps and parameters were the same, and will not be repeated here. The final hair dye composition was obtained.

[0028] Comparative Example 4: Compared with Example 1, this comparative example did not add sodium hyaluronate in the preparation of the second component. All other steps and parameters were the same, and will not be repeated here. The final hair dye composition was obtained.

[0029] Comparative Example 5: Compared with Example 1, this comparative example did not undergo heat inactivation treatment during the preparation of the microbial composition. All other steps and parameters were the same, and will not be repeated here. The final hair dye composition was obtained.

[0030] Comparative Example 6: Compared with Example 1, this comparative example only replaces "heat inactivation treatment at 90°C for 10 min" with "heat inactivation treatment at 121°C for 10 min". All other steps and parameters are the same, and will not be repeated in this comparative example. The final hair dyeing composition is obtained.

[0031] Performance testing: 1. Hair dyeing method: First, component A was applied to white hair with an L value of 66.2 and a color intensity of 10 and left to stand for 30 minutes. Then, component B was applied and left to stand for 20 minutes. The mass ratio of component A to component B was 1:1.2-1.3. Finally, the hair was washed with shampoo and air-dried to obtain black hair from Examples 1-5 and Comparative Examples 1-6, respectively. 2. Hair blackness: The blackness values ​​of black hair from Examples 1-5 and Comparative Examples 1-6 were tested three times using a colorimeter, and the average value was taken. At the same time, the color was obtained by comparing with a color chart. The blackness L value of normal black hair ranges from 16 to 18, and the color ranges from 2 to 3. The smaller the L value or color, the darker the hair color. 3. Wash fastness: After 10 washes, the color fastness to washing was tested. The test method was based on ISO 105-C01-C05-1989 "Textiles - Tests for color fastness to washing: Tests 1-5" with modifications. Specifically, 2g of hair sample was placed in hair dye, rubbed and fixed at 30°C for 10 minutes, mechanically stirred, rinsed and dried, and the color fastness to washing was evaluated using a denier meter.

[0032] Hair tensile strength: The diameter of the hair strands was measured using an SN-1200W high-definition camera. Specifically, the average diameter of three locations in the middle section was taken. Then, 30 hair strands with a diameter difference within 10 μm from 11 hair bundles obtained in Examples 1-5 and Comparative Examples 1-6 were used to perform single fiber strength tests using a fiber strength tester to obtain the tensile strength of each group of hair strands. Tensile strength of hair (σ) = F b / S o ; In the formula, σ is the tensile strength, F b The maximum force that the specimen withstands when it breaks, S o This represents the original cross-sectional area of ​​the sample. Anti-frizz performance: Eleven hair bundles obtained from Examples 1-5 and Comparative Examples 1-6 were placed in an environment with a relative humidity of approximately 80% (simulating a rainy day), and the changes in the curliness and frizz of the hair bundles and the volume of the hair bundles were recorded after 4 hours in a high relative humidity environment.

[0033] Table 1. Summary of experimental data from Examples 1-5 and Comparative Examples 1-6

[0034] Data Analysis: As shown in Table 1, the hair dye composition prepared by this invention is easy to color, has high color fastness to washing, and strong resistance to damage. This may be because the 5,6-dihydroxyindole in the first component of this invention acts on the keratin fibers of the hair, easily penetrates into the hair medulla, and is not easy to fade after washing. The hydrogen peroxide stabilizer in the second component can effectively prevent the decomposition of hydrogen peroxide and improve the storage stability of the hair dye composition. The anti-damage composition is obtained by mixing sodium hyaluronate, fermented extract of Japanese purple palm fruit, extract of brown finger algae, and microbial composition. Lactobacillus plantarum L30 and Bifidobacterium 070310 have a certain repairing effect on damaged hair. The microbial composition after heat inactivation treatment has anti-damage and repair effects on hair, while having high stability and not easily ineffective due to the influence of hydrogen peroxide and hydrogen oxidizing agent. When temperatures are too high, the properties of the microbial metabolites will change significantly. Excessive heating will lead to the degradation of heat-sensitive active ingredients and a weakening of their biological efficacy, thus hindering their repair function. Japanese purple pearl fruit fermented extract is rich in effective ingredients that nourish, repair, and have antioxidant effects on hair and scalp, and can effectively repair damaged hair strands. Furthermore, sodium hyaluronate has extremely strong water absorption and hydrogen bond formation capabilities; its low number-average molecular weight allows it to penetrate deep into the hair shaft, effectively repairing damaged protein structures. The extract of *Phaeodactylum tricornutum* promotes hair follicle renewal and hair growth by increasing the expression of various keratins in the outer root sheath cells of hair follicles. The synergistic effect of sodium hyaluronate, Japanese purple pearl fruit fermented extract, *Phaeodactylum tricornutum* extract, and the microbial composition achieves anti-damage and repair effects.

[0035] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of the invention is limited to these examples; within the framework of the invention, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.

[0036] This invention is intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A hair dye composition based on hydrogen peroxide, characterized in that, The hydrogen peroxide-based hair dye composition comprises a first component and a second component; The mass ratio of the first component to the second component is 1:1.2-1.3; The first component comprises the following parts by weight of raw materials: 5,6-Dihydroxyindole 6-9 parts; C16-18 alcohol 9-17.5 parts; cetyl PEG 3-5 parts; olive oil 15-20 parts; hexadecyltrimethylammonium chloride 1-3 parts; cocamidobenzene 2-3 parts; Tween 80 1-2 parts; Cysteine ​​3.3-6.8 parts; threonine 1-2 parts; xanthan gum 1-2 parts; hydrolyzed protein 2-3 parts; water-soluble lanolin 2-3 parts; deionized water 88-97 parts; 10% citric acid aqueous solution; The second component comprises the following parts by weight of raw materials: Hydrogen peroxide 1.5-3.2 parts; xanthan gum 1-2 parts; glycerin 5-7 parts; oleyl alcohol 5-7 parts; Hydrogen peroxide stabilizer 0.01-0.03 parts; anti-damage composition 5.5-7.8 parts; deionized water 93-110 parts; The anti-damage composition is obtained by mixing sodium hyaluronate, fermented extract of Japanese purple palm fruit, extract of brown finger algae, and a microbial composition. The microbial composition was prepared from Lactobacillus plantarum L30 and Bifidobacterium 070310.

2. The hydrogen peroxide-based hair dye composition according to claim 1, characterized in that, The mass ratio of sodium hyaluronate, Japanese purple palm fruit fermented extract, triangular brown finger algae extract and microbial composition is 1-1.5:7.2-9.6:3-6:3.5-4.

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3. The hydrogen peroxide-based hair dye composition according to claim 1, characterized in that, The preparation method of the microbial composition is as follows: Lactobacillus plantarum L30 and Bifidobacterium 070310 were activated in sterile MRS culture medium at 37℃ for 22-24 h, centrifuged at 4000-5000 rpm for 15-20 min, washed three times with sterile water, and resuspended in phosphate buffer solution to a concentration of 1×10⁻⁶. 10 -3.5×10 10 The concentration of CFU / mL was then subjected to heat inactivation to obtain the microbial composition.

4. The hydrogen peroxide-based hair dye composition according to claim 3, characterized in that, The MRS culture medium formula is as follows: 10g peptone, 10g beef extract, 5g yeast extract, 20g glucose, 1mL Tween-80, 2g diammonium hydrogen citrate, 5g sodium acetate, 2g dipotassium hydrogen phosphate, 0.58g magnesium sulfate heptahydrate, 0.25g manganese sulfate tetrahydrate, 1000mL distilled water, pH 6.

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5. The hydrogen peroxide-based hair dye composition according to claim 3, characterized in that, The heat inactivation treatment is performed at a temperature of 90-100℃ for 10 minutes.

6. The hydrogen peroxide-based hair dye composition according to claim 1, characterized in that, The preparation method of the *Phaeodactylum tricornutum* extract is as follows: Add *Phaeodactylum tricornutum* to a 95% ethanol aqueous solution, add 30% sodium hydroxide solution to adjust the pH of the system to 13, and reflux extract at 75-80℃ for 20-30 min under a nitrogen atmosphere. Cool to 18-20℃, filter, add deionized water and stir to form an aqueous-ethanol phase. Perform two-phase extraction, elute the aqueous-ethanol phase with n-heptane, collect the lower layer to obtain fatty acid salts, extract twice, acidify the lower layer to pH 2.5 with sulfuric acid, elute with n-heptane, and rotary evaporate to obtain *Phaeodactylum tricornutum* extract.

7. The hydrogen peroxide-based hair dye composition according to claim 6, characterized in that, The ratio of the amount of *Phaeodactylum tricornutum*, 95% ethanol aqueous solution, and deionized water used is 10-20g: 280-500mL: 1500mL.

8. The hydrogen peroxide-based hair dye composition according to claim 1, characterized in that, The fermented extract of Japanese purple fruit is obtained by enzymatic hydrolysis of Japanese purple fruit with cellulase, fermentation with Lactobacillus plantarum and active dry yeast, and subsequent ripening treatment. The number-average molecular weight of the sodium hyaluronate is 10-40 kDa.

9. The hydrogen peroxide-based hair dye composition according to claim 1, characterized in that, The hydrogen peroxide stabilizer is at least one of phenacetin, disodium hydrogen phosphate, and disodium dihydrogen pyrophosphate.

10. The method for preparing the hydrogen peroxide-based hair dye composition according to any one of claims 1-9, characterized in that, Includes the following steps: Step S1. Mix C16-18 alcohol, cetyl PEG, cocamidocyanate, olive oil and hexadecyltrimethylammonium chloride and heat to 70-80℃ to obtain the oil phase; mix cysteine, Tween 80, threonine, xanthan gum and deionized water at 70-75℃ until homogeneous, then cool to 35-40℃ and add hydrolyzed protein and water-soluble lanolin to obtain the aqueous phase; Step S2. Add the oil phase to the aqueous phase and stir homogenously at 40-50℃ and keep warm for 10-20 min. Add 10% citric acid aqueous solution to adjust the pH of the system to 4, then add 5,6-dihydroxyindole and mix well to obtain the first component. Step B3. Mix glycerin, oleyl alcohol, xanthan gum, hydrogen peroxide stabilizer, anti-damage composition and deionized water at 60-70℃ until homogeneous. After the system cools down to 30-35℃, add hydrogen peroxide to obtain the second component, which is the hydrogen peroxide-based hair dye composition.

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