Fabric cleaning agent with anti-HPV (human papillomavirus) effect and preparation method thereof

Abstract

The invention provides a fabric cleaning agent with an anti-HPV effect and a preparation method thereof, and relates to the technical field of cleaning agents. According to the Gemini type quaternary ammonium salt surfactant composition disclosed by the invention, through the effects of the Gemini type quaternary ammonium salt surfactants with two specific structures, high synergy of washing and disinfecting functions is realized, excellent dirt-removing power can be achieved only by virtue of an optimized and compounded surfactant system, and biological enzymes which are easy to inactivate and difficult to stabilize do not need to be introduced; in a high-content surfactant system and in a weak acid environment, the compound still has an efficient and reliable antiviral effect on the human papilloma virus, and meets the dual requirements of public health and personal care; and the formula is mild and weakly acidic and fits the physiological environment of the skin, so that the use experience and the effect exertion are favorably improved.

Description

Technical Field

[0001] This invention relates to the technical field of cleaning agents, and in particular to a fabric cleaning agent with anti-HPV activity and its preparation method, specifically to a Gemini-type quaternary ammonium salt surfactant composition and a fabric cleaning agent with anti-HPV virus activity. Background Technology

[0002] Cervical cancer has become a significant threat to women's health worldwide, ranking as the second leading cause of cancer death among women. High-risk human papillomavirus (HR-HPV) is considered the primary factor in the development of cervical cancer lesions. Human papillomavirus (HPV) is not a single, specific virus, but rather comprises over 200 subtypes, 14 of which are associated with cancer development. HPV can be divided into low-risk and high-risk types. Common low-risk types include HPV6 and HPV11, while common high-risk types include HPV16, HPV18, HPV31, HPV33, HPV52, and HPV58. In my country, the HPV infection rate among women is approximately 14%, with an overall high-risk HPV infection rate of 23.8%. The top five most common HPV subtypes are HPV16, 58, 52, 18, and 33.

[0003] As living standards improve, women are paying more attention to the effectiveness of underwear detergents. They not only want it to effectively remove blood stains and other contaminants, but also demand antibacterial and antiviral properties. Most underwear detergents on the market claim antibacterial effects, but few claim to inactivate viruses. One brand claims to be anti-HPV, but it only lists the virus removal rate, not the virus inactivation rate. Most disinfectant products on the market that claim to inactivate HPV are sprays or low-concentration surfactant systems.

[0004] (1) Hypochlorous acid disinfectant uses hypochlorous acid as a disinfectant with an effective chlorine content of 100-130 mg / L. The undiluted solution has a 99.9% inactivation rate against HPV16 virus after 1 minute of action. However, this is a disinfectant spray and cannot be used as a fabric cleaner. Moreover, hypochlorous acid is unstable and its content usually decreases within 3-6 months. Furthermore, the strong oxidizing properties of hypochlorous acid make it incompatible with fragrances, and the chlorine odor released from the decomposition of hypochlorous acid is unfriendly to consumers.

[0005] (2) Disinfectants containing phenolic bactericides, such as those using 4.3%~5.3% PCMX as a bactericide, diluted 1:5 and left to act for 3 minutes, have an inactivation rate of 99.99% against HPV16 virus. Although the virus inactivation effect is excellent, this product has a low surfactant content and is a disinfectant, not a laundry detergent.

[0006] (3) Disinfectants containing compound quaternary ammonium salts, for example, using 4.5%~5.5% compound quaternary ammonium salts as bactericides (dimethylbenzylalkylammonium chloride and dilauryl dimethylammonium chloride) as bactericides, the stock solution, after 10 minutes of action, has an inactivation rate of 99.99% against HPV16 virus. However, the bactericide content is as high as 4.5%~5.5%, and the pH value is neutral. Among them, the quaternary ammonium salt bactericide has a viscosity-reducing effect, so the product viscosity is <500 CP. The bactericide content is as high as 4.5%~5.5%, indicating that other surfactants have a significant reducing effect on the bactericidal effect of the quaternary ammonium salt bactericide, so it is necessary to increase the bactericide to 4.5%~5.5% to ensure the bactericidal effect. Moreover, in order to achieve the decontamination effect, the total active matter is as high as 25% or more. It should be noted that the cost of the product is directly proportional to the total active matter content, so the cost of this technical solution is very high.

[0007] Furthermore, according to published literature, ordinary quaternary ammonium salt bactericides show better bactericidal effects under neutral or slightly alkaline conditions, but their bactericidal effect decreases when the pH is adjusted to slightly acidic. Moreover, compound quaternary ammonium salt bactericides generally require application in nonionic surfactant systems; if applied in anionic surfactant systems, the strong interaction between the cations and anions will significantly reduce the bactericidal effect.

[0008] Maintaining a high level of anti-HPV efficacy in high-surfactant systems is difficult, meaning it's challenging to inactivate HPV while simultaneously cleaning clothing. According to the "Disinfection Technical Specifications" (2002 edition), the order of microbial sensitivity to disinfectants from high to low is generally considered to be: (1) lipophilic viruses (viruses with lipid membranes, also known as enveloped viruses), such as hepatitis B virus and influenza virus; (2) vegetative bacteria; (3) fungi; and (4) hydrophilic viruses, such as hepatitis A virus and poliovirus. Among these, HPV virus is a hydrophilic virus. If a disinfectant exhibits good killing ability against lipophilic viruses, bacteria, and fungi, it does not necessarily mean that it has good inactivation ability against hydrophilic viruses. Moreover, high levels of surfactants often significantly reduce the bactericidal effect of quaternary ammonium salt bactericides. Using quaternary ammonium salt bactericides alone is not enough to effectively kill hydrophilic HPV viruses, let alone in high-surfactant systems where the microbial inactivation effect of quaternary ammonium salt bactericides is weakened.

[0009] Furthermore, introducing other quaternary ammonium salts (cationic surfactants) into anionic laundry detergent formulations often leads to a decrease in cleaning or antibacterial effects. Quaternary ammonium salts, as cationic surfactants, have a positively charged core; while the main components of anionic laundry detergents (such as LAS and AES) are negatively charged. When they meet, a strong electrostatic adsorption occurs, disrupting the micelle structure and rendering the quaternary ammonium salt inactive. For example, quaternary ammonium salts need to adsorb onto the surface of bacteria to function, but anionic surfactants preemptively occupy these sites, thus "neutralizing" their bactericidal ability.

[0010] While some existing technologies have disclosed that compound quaternary ammonium salt disinfectants have a certain inactivation effect on poliovirus type I vaccine strains, these require the addition of a certain amount of synergist. Without synergist, even increasing the concentration of the disinfectant and extending the contact time cannot achieve the corresponding inactivation effect. Using the compound quaternary ammonium salt alone is also insufficient for effective inactivation. Other studies have shown that disinfectants formulated with specific mono- and di-fatty acid long-chain quaternary ammonium salts have poor inactivation effects on this virus. Furthermore, pH value significantly affects the disinfection efficacy of quaternary ammonium salt disinfectants. These disinfectants are more effective in alkaline environments; the higher the pH, the stronger the bactericidal effect. In acidic environments, their bactericidal effect decreases significantly, or even fails to achieve effective inactivation.

[0011] CN117965247A discloses a highly effective antibacterial laundry detergent and its preparation method. The laundry detergent comprises 5%–25% nonionic surfactant, 0.1%–25% cationic Gemini surfactant, 1%–4% viscosity modifier, 0%–1% enzyme, 0%–0.1% stabilizer, 0%–0.3% preservative, 0%–0.05% colorant, and 0.1%–1.5% fragrance, with the balance being deionized water. The cationic Gemini surfactant is a long-chain alkyl quaternary ammonium salt containing a five-membered heterocycle, synthesized through a multi-step reaction. While the laundry detergent prepared by this invention possesses excellent antibacterial and detergency properties, it has no inactivating effect on hydrophilic viruses.

[0012] CN114403144A discloses a quaternary ammonium salt composition for killing microorganisms, its preparation method, and its application. The quaternary ammonium salt composition of this invention may or may not contain sodium free ions; when the quaternary ammonium salt composition contains sodium free ions, the molar number of sodium free ions in the quaternary ammonium salt composition is <0.1 mol / 100g; when the quaternary ammonium salt composition contains sodium free ions, and the molar number of sodium free ions is ≥0.001 mol / 100g, the quaternary ammonium salt composition also simultaneously contains potassium free ions and chelating ions / ligands. This invention employs a quaternary ammonium salt synergistic system, selectively adding ion types, and can also be compounded with non-quaternary ammonium salt surfactants, thereby making the quaternary ammonium salt composition highly effective in inactivating hydrophilic viruses, such as poliovirus. However, the quaternary ammonium salt system used (either alone or in combination with nonionic surfactants) is less effective at inactivating non-enveloped hydrophilic viruses; moreover, quaternary ammonium salts need to be used in combination with a large amount of low flash point raw materials (such as ethanol), which can easily cause combustion and explosion, posing a safety risk.

[0013] In summary, existing technologies show that the inactivation effect of quaternary ammonium salts or complex quaternary ammonium salts on hydrophilic viruses is relatively weak. Highly efficient inactivation can only be achieved by adding synergists, optimizing ion types, and under high pH conditions. While existing detergents based on Gemini-type surfactants possess strong antibacterial properties, research and data on their effectiveness in inactivating hydrophilic viruses are still relatively lacking.

[0014] Therefore, there is an urgent need to provide a solution for a weakly acidic fabric cleaning agent that can exist in a high surfactant system and has a good HPV16 virus inactivation effect.

[0015] In view of this, the present invention is hereby proposed. Summary of the Invention

[0016] The purpose of this invention is to provide a fabric cleaning agent with anti-HPV virus activity and its preparation method, aiming to solve the technical problem that cationic surfactant components are easily inhibited by nonionic or anionic surfactants and lose their activity under conditions of high surfactant content and weak acidity, resulting in extremely poor inactivation effect on hydrophilic human papillomavirus; at the same time, it also breaks through the technical bottleneck of traditional quaternary ammonium salt systems that rely on alkaline environment, require additional synergists or high-risk adjuvants, and are difficult to achieve efficient decontamination and reliable virus inactivation simultaneously.

[0017] In order to achieve the above-mentioned objectives of the present invention, the following technical solution is adopted: In a first aspect, the present invention provides a Gemini-type quaternary ammonium salt surfactant composition, comprising the Gemini-type quaternary ammonium salt shown in Formula I and the Gemini-type quaternary ammonium salt shown in Formula II; The structural formula of the Gemini-type quaternary ammonium salt represented by Formula I is shown below:

[0018] Formula I; In Formula I, R1 is selected from any one of C1 to C3 spar groups, R2 is selected from any one of C12 to C18 alkyl groups, and X is selected from halogen atoms; The structural formula of the Gemini-type quaternary ammonium salt represented by Formula II is shown below:

[0019] Formula II; In Formula II, R1' and R3' are each independently selected from any one of the C1 to C3 spar groups, R2' is selected from any one of the C12 to C18 alkyl groups, and Y is selected from a halogen atom.

[0020] Furthermore, in Formula I, R1 is selected from ethylene-based or propylene-based, preferably propylene-based.

[0021] Furthermore, in Formula I, R2 is selected from C16 to C18 alkyl groups, preferably C18 alkyl groups.

[0022] Furthermore, in Formula I, the halogen atom is selected from any one of F, Cl, Br, and I, preferably Cl.

[0023] Furthermore, in Formula II, R1' is selected from ethylene-based or propylene-based, preferably propylene-based.

[0024] Furthermore, in Formula II, R3' is selected from ethylene-based or propylene-based, preferably propylene-based.

[0025] Furthermore, in Formula II, R2' is selected from C16 to C18 alkyl groups, preferably C18 alkyl groups.

[0026] Furthermore, in Formula II, the halogen atom is selected from any one of F, Cl, Br, and I, preferably Cl.

[0027] Furthermore, the Gemini-type quaternary ammonium salt surfactant composition is composed of propylene bis(octadecyl dimethyl ammonium chloride) and propylene bis(octadecamidopropyl dimethyl ammonium chloride).

[0028] Furthermore, the mass ratio of the Gemini-type quaternary ammonium salt shown in Formula I to the Gemini-type quaternary ammonium salt shown in Formula II is 1:(0.5~5), preferably 1:(1~3), and even more preferably 1:2.

[0029] In a second aspect, the present invention provides the use of the Gemini-type quaternary ammonium salt surfactant composition as described in the first aspect in the preparation of a cleaning product for use against HPV viruses.

[0030] Thirdly, the present invention provides a fabric cleaning agent with anti-HPV virus activity, wherein the fabric cleaning agent with anti-HPV virus activity comprises the Gemini-type quaternary ammonium salt surfactant composition as described in the first aspect.

[0031] Furthermore, the amount of the Gemini-type quaternary ammonium salt surfactant composition added accounts for 0.01% to 5% of the total mass of the fabric cleaning agent, preferably 0.5% to 2%, and more preferably 1%.

[0032] Furthermore, the fabric cleaning agent also includes any one or a combination of at least two of the following: nonionic surfactants, anionic surfactants, and zwitterionic surfactants.

[0033] Furthermore, the nonionic surfactant includes any one or a combination of at least two of alkyl glycosides, fatty alcohol polyoxyethylene ethers, and alkanolamides.

[0034] Furthermore, the amount of the nonionic surfactant added accounts for 5% to 40% of the total mass of the fabric cleaning agent.

[0035] Furthermore, the anionic surfactant includes any one or a combination of at least two of alkyl ether carboxylates and alkyl ether sulfates.

[0036] Furthermore, the amount of the anionic surfactant added accounts for 5% to 25% of the total mass of the fabric cleaning agent.

[0037] Furthermore, the zwitterionic surfactant includes any one or a combination of at least two of cocamidopropyl dimethyl betaine and alkylamidopropyl amine oxides; Furthermore, the amount of the zwitterionic surfactant added accounts for 1% to 25% of the total mass of the fabric cleaning agent.

[0038] Furthermore, the fabric cleaning agent also includes a thickener.

[0039] Furthermore, the thickener comprises any one or a combination of at least two of PEG-150 distearate, PEG-120 methyl glucoside dioleate, PEG-120 methyl glucoside trioleate, and PEG-150 pentaerythritol tetrastearate.

[0040] Furthermore, the amount of the thickener added accounts for 0.5% to 5% of the total mass of the fabric cleaning agent.

[0041] Furthermore, the fabric cleaning agent also includes a pH adjuster.

[0042] Furthermore, the pH adjuster is any one or a combination of at least two of citric acid, lactic acid, sodium citrate, and sodium lactate.

[0043] Furthermore, the pH of the fabric cleaning agent is 4.5 to 5.5.

[0044] Fourthly, the present invention provides a method for preparing a fabric cleaning agent with anti-HPV virus activity as described in the third aspect, the preparation method comprising: The Gemini-type quaternary ammonium salt surfactant composition was dissolved in water to obtain the fabric cleaning agent with anti-HPV virus activity.

[0045] Further, the preparation method includes: A nonionic surfactant, a Gemini-type quaternary ammonium salt surfactant composition, and a thickener are dissolved in water at 60-80°C, and then a pH adjuster is added to adjust the pH to a slightly acidic state to obtain the fabric cleaning agent with anti-HPV virus activity.

[0046] Further, the preparation method includes: An anionic surfactant, amphoteric surfactant, nonionic surfactant, a Gemini-type quaternary ammonium salt surfactant composition and a thickener are dissolved in water at 60-80°C, and then a pH adjuster is added to adjust the pH to weakly acidic to obtain the fabric cleaning agent with anti-HPV virus activity.

[0047] Compared with the prior art, the present invention has the following beneficial effects: (1) The surfactant composition of the present invention, through the synergistic cooperation of the Gemini-type quaternary ammonium salt of Formula I and the Gemini-type quaternary ammonium salt of Formula II with specific structures, breaks through the technical inertia that quaternary ammonium salt disinfectant components generally rely on alkaline environment to exert their activity. It has a stable and efficient anti-HPV virus effect in a weakly acidic environment, makes up for the defect of weakened electrostatic adsorption under acidic conditions, and makes the inactivation effect independent of pH increase, significantly improving product safety and human affinity.

[0048] (2) The surfactant composition of the present invention overcomes the bactericidal inhibition caused by high surfactant content by synergistic assembly of Gemini-type quaternary ammonium salt of Formula I and Gemini-type quaternary ammonium salt of Formula II with specific structure. In the coexistence environment of high concentration of nonionic or anionic surfactants, it forms an active aggregate with interfacial selectivity, effectively avoids the electrostatic neutralization and micelle encapsulation effect of anionic surfactants, optimizes the interaction ability between molecules and virus surface, and makes the anti-HPV virus ability still stably expressed when the main cleaning ingredient has a high proportion, truly realizing the integration of cleaning and antiviral functions.

[0049] (3) The surfactant composition of the present invention uses the Gemini-type quaternary ammonium salt of Formula I and the Gemini-type quaternary ammonium salt of Formula II to synergistically combine, so that the formulation completely eliminates easily inactivated biological components such as proteases and lipases and strong oxidizing disinfectants such as hypochlorous acid. It relies solely on the surfactant itself to achieve the dual functions of detergency and inactivation, avoiding the performance degradation caused by enzyme denaturation and failure. At the same time, it avoids the risk of toxic byproducts generated by the reaction of oxidants with organic matter. The system has good compatibility and high stability, and the activity remains consistent during long-term storage and washing. It has excellent cleaning power, low irritation and environmental friendliness, and takes into account both cleaning performance and biological safety. Detailed Implementation

[0050] Unless otherwise defined herein, the scientific and technical terms used in conjunction with this invention shall have the meanings commonly understood by one of ordinary skill in the art. The meaning and scope of terms shall be clear; however, in any case of potential ambiguity, the definitions provided herein shall prevail over any dictionary or foreign definitions. In this application, unless otherwise stated, the use of "or" means "and / or". Furthermore, the use of the term "comprising" and other forms is non-limiting.

[0051] Generally, the nomenclature and techniques used in cell and tissue culture, molecular biology, immunology, microbiology, genetics, and protein and nucleic acid chemistry and hybridization, together with those described herein, are those well-known and commonly used in the art. Unless otherwise stated, the methods and techniques of the present invention are generally carried out according to conventional methods well-known in the art and described in various general and more specific references, which are cited and discussed throughout this specification. Enzymatic reactions and purification techniques are carried out according to the manufacturer's instructions, as commonly practiced in the art, or as described herein. The nomenclature, laboratory procedures, and techniques used in analytical chemistry, synthetic organic chemistry, and medical and medicinal chemistry, together with those described herein, are those well-known and commonly used in the art.

[0052] [Terminology Explanation] Unless otherwise stated, the following terms have the following meanings: The term "independently" means that at least two groups (or ring systems) in a structure with the same or similar value ranges can have the same or different meanings under certain circumstances.

[0053] The terms “contain” and “include” are used in their open, non-restrictive sense.

[0054] The term "alkyl" refers to a monovalent straight-chain or branched alkane group composed only of carbon and hydrogen atoms, without unsaturation, and connected to other segments by a single bond, including but not limited to butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecanyl, octadecyl, etc. For example, the term "C12-C18 alkyl" in this invention refers to a saturated monovalent straight-chain or branched alkane group containing 12 to 18 carbon atoms, specifically a C12, C13, C14, C15, C16, C17, or C18 straight-chain or branched alkyl group.

[0055] The term "alkyl group" refers to a divalent straight-chain or branched alkane group, also known as an alkylene group, which consists only of carbon and hydrogen atoms, contains no unsaturation, and is connected to other segments through two single bonds, including but not limited to methyl alkyl, ethylene alkyl, propylene alkyl, butyl alkyl, pentyl alkyl, and hexyl alkyl. For example, the term "C1~C3 alkyl group" in this invention refers to a saturated monovalent straight-chain or branched alkyl group containing 1 to 3 carbon atoms, specifically a straight-chain or branched alkyl group of methyl alkyl, ethylene alkyl, or propylene alkyl.

[0056] The term "halogen atom" refers to a nonmetallic element in Group VIIA of the periodic table, which is a single-atom nonmetallic bonding group composed only of halogen single atoms and can be connected to other segments through covalent bonds, including but not limited to fluorine, chlorine, bromine, and iodine. For example, the term "halogen" in this invention can be independently selected from one or more of fluorine, chlorine, bromine, and iodine, which are anions that form quaternary ammonium salts.

[0057] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0058] In a first aspect, the present invention provides a Gemini-type quaternary ammonium salt surfactant composition, comprising the Gemini-type quaternary ammonium salt shown in Formula I and the Gemini-type quaternary ammonium salt shown in Formula II; The structural formula of the Gemini-type quaternary ammonium salt represented by Formula I is shown below:

[0059] Formula I; In Formula I, R1 is selected from any one of C1 to C3 spar groups, R2 is selected from any one of C12 to C18 alkyl groups, and X is selected from halogen atoms; The structural formula of the Gemini-type quaternary ammonium salt represented by Formula II is shown below:

[0060] Formula II; In Formula II, R1' and R3' are each independently selected from any one of the C1 to C3 spar groups, R2' is selected from any one of the C12 to C18 alkyl groups, and Y is selected from a halogen atom.

[0061] It should be noted that the Gemini-type quaternary ammonium salt surfactant composition of this invention utilizes a unique molecular-level synergistic mechanism between two structurally different Gemini surfactants to achieve highly efficient targeted intervention against recalcitrant hydrophilic viruses (HPV) in a weakly acidic, highly surfactant environment that is traditionally considered mutually exclusive, exhibiting stable and efficient anti-HPV virus activity. Structurally, both use a propylene group as a bis-quaternary ammonium salt core for bridging. The positively charged quaternary ammonium group can target and bind to the negative potential point of the HPV hydrophilic capsid. In a weakly acidic environment, the positive charge is more stable, significantly improving the electrostatic binding efficiency, breaking the traditional inhibition of quaternary ammonium salt activity by weak acids, and inducing viral aggregation and inactivation. The former has a strong hydrophobicity of the octadecyl chain, which easily forms micelles to encapsulate virus particles in a highly surfactant environment, and the bis-octadecyl group forms steric hindrance, blocking the binding of the virus to the cell; the latter's amamidopropyl group introduces hydrogen bonding sites, which can precisely bind to the hydrophilic region of the viral protein, compensating for the former's weakness in hydrophilicity. The combination of the two achieves a synergistic effect of hydrophobic encapsulation and hydrophilic targeting. The highly active environment does not destroy the core function of the quaternary ammonium salt. Instead, it enhances the targeted adsorption and intervention of hydrophilic HPV through micellar solubilization, thus achieving efficient inactivation.

[0062] As an optional implementation, in Formula I, R1 is selected from ethylene-based or propylene-based.

[0063] In a preferred embodiment, R1 in Formula I is a propylene group.

[0064] As an optional implementation, in Formula I, R2 is selected from C16-C18 alkyl groups.

[0065] In a preferred embodiment, R2 in Formula I is a C18 alkyl group.

[0066] As an optional implementation, in Formula I, the halogen atom is selected from any one of F, Cl, Br, and I.

[0067] In a preferred embodiment, the halogen atom in Formula I is Cl.

[0068] As an optional implementation, in Formula II, R1' is selected from ethylene-based or propylene-based.

[0069] In a preferred embodiment, R1' in Formula II is a propylene group.

[0070] As an optional implementation, in Formula II, R3' is selected from ethylene-based or propylene-based.

[0071] In a preferred embodiment, R3' in Formula II is a propylene group.

[0072] As an optional implementation, in Formula II, R2' is selected from C16-C18 alkyl groups.

[0073] In a preferred embodiment, R2' in Formula II is a C18 alkyl group.

[0074] As an optional implementation, in Formula II, the halogen atom is selected from any one of F, Cl, Br, and I.

[0075] As an optional implementation, in Formula II, the halogen atom is Cl.

[0076] In a preferred embodiment, the Gemini-type quaternary ammonium salt surfactant composition is composed of propylene bis(octadecyl dimethyl ammonium chloride) and propylene bis(octadecylamidopropyl dimethyl ammonium chloride).

[0077] Propylene bis(octadecyldimethylammonium chloride) is a Gemini quaternary ammonium salt cationic surfactant. Its molecule contains two hydrophobic groups and two hydrophilic groups, linked by chemical bonds through connecting groups. This results in a very tight bond between the two surfactant monomers. This structure enhances the hydrophobic effect of the hydrocarbon chains while significantly weakening the repulsive effect between the hydrophilic groups due to the chemical bonds. Therefore, it possesses superior physicochemical properties, such as a more outstanding ability and efficiency in reducing the surface tension of aqueous solutions, and exhibits high surface activity.

[0078] Among them, propylene-based bis(octadecamidopropyl dimethylammonium chloride) is a Gemini quaternary ammonium salt cationic surfactant. Due to the introduction of amide bonds, its molecular arrangement becomes irregular, thereby lowering the CMC (critical micelle concentration). This results in a more pronounced ability and efficiency in reducing the surface tension of aqueous solutions. It exhibits high surface activity, a very low Krafft point, and good Ca soap dispersing power, wetting ability, foaming and foam stability, solubilizing ability, antibacterial ability, and detergency. It is currently one of the most rapidly developing surfactant series in the world. Furthermore, compared to traditional surfactants, it is more easily adsorbed at the interface between two phases, with an adsorption capacity 10 to 1000 times that of traditional surfactants. Therefore, it has excellent efficiency and ability in reducing surface tension, foaming, stabilizing foam, and emulsifying. Moreover, it has a low critical micelle concentration (CMC), which is only 1 / 10 to 1 / 100 of that of traditional surfactants. This means that it has low irritation and has a strong solubilizing effect and cost advantage. In particular, it can be compounded with almost all anionic surfactants without precipitation, and adding a small amount (1% to 25%) has a significant synergistic effect.

[0079] It should be noted that propylene-based bis(octadecyldimethylammonium chloride) provides strong hydrophobic insertion capability and stable dication configuration, while propylene-based bis(octadecylamidopropyldimethylammonium chloride) introduces polar recognition sites and hydrogen bonding due to the amide bond. The two achieve rigid-flexible complementarity and polar synergy under the unified configuration of the propylene linker. The octadecyl chain length takes into account both viral capsid compatibility and micellar integration stability, enabling the composite system to form an active microstructure with both targeted binding and physical destruction functions during interfacial self-assembly.

[0080] As an optional implementation, the mass ratio of the Gemini-type quaternary ammonium salt shown in Formula I to the Gemini-type quaternary ammonium salt shown in Formula II is 1:(0.5~5), for example, it can be 1:0.5, 1:0.6, 1:0.8, 1:1, 1:1.2, 1:1.4, 1:1.6, 1:1.8, 1:2, 1:2.2, 1:2.4, 1:2.6, 1:2.8, 1:3, 1:3.2, 1:3.4, 1:3.6, 1:3.8, 1:4, 1:4.2, 1:4.4, 1:4.6, 1:4.8, 1:5, etc.

[0081] In a preferred embodiment, the mass ratio of the Gemini-type quaternary ammonium salt shown in Formula I to the Gemini-type quaternary ammonium salt shown in Formula II is 1:(1~3).

[0082] As a more preferred embodiment, the mass ratio of the Gemini-type quaternary ammonium salt shown in Formula I to the Gemini-type quaternary ammonium salt shown in Formula II is 1:2.

[0083] In a second aspect, the present invention provides the use of the Gemini-type quaternary ammonium salt surfactant composition as described in the first aspect in the preparation of a cleaning product for use against HPV viruses.

[0084] Thirdly, the present invention provides a fabric cleaning agent with anti-HPV virus activity, wherein the fabric cleaning agent with anti-HPV virus activity comprises the Gemini-type quaternary ammonium salt surfactant composition as described in the first aspect.

[0085] As an optional implementation, the amount of the Gemini-type quaternary ammonium salt surfactant composition added accounts for 0.01% to 5% of the total mass of the fabric cleaning agent, for example, it can be 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc.

[0086] In a preferred embodiment, the amount of the Gemini-type quaternary ammonium salt surfactant composition added accounts for 0.5% to 2% of the total mass of the fabric cleaning agent.

[0087] As a more preferred embodiment, the amount of the Gemini-type quaternary ammonium salt surfactant composition added accounts for 1% of the total mass of the fabric cleaning agent.

[0088] As an optional implementation, the fabric cleaning agent may further include any one or a combination of at least two of nonionic surfactants, anionic surfactants, and zwitterionic surfactants.

[0089] As an optional implementation, the nonionic surfactant includes any one or a combination of at least two of alkyl glycosides, fatty alcohol polyoxyethylene ethers, and alkanolamides.

[0090] As an optional implementation, the amount of the nonionic surfactant added accounts for 5% to 40% of the total mass of the fabric cleaning agent, for example, it can be 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, etc.

[0091] As an optional embodiment, the anionic surfactant includes any one or a combination of at least two of alkyl ether carboxylates and alkyl ether sulfates.

[0092] As an optional implementation, the amount of the anionic surfactant added accounts for 5% to 25% of the total mass of the fabric cleaning agent, for example, it can be 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, etc.

[0093] As an optional implementation, the zwitterionic surfactant includes any one or a combination of at least two of cocamidopropyl dimethyl betaine and alkylamidopropyl amine oxides.

[0094] As an optional implementation, the amount of the amphoteric surfactant added accounts for 1% to 25% of the total mass of the fabric cleaning agent, for example, it can be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, etc.

[0095] As an optional implementation, the fabric cleaning agent also includes a thickener.

[0096] As an optional embodiment, the thickener includes any one or a combination of at least two of PEG-150 distearate, PEG-120 methylglucose dioleate, PEG-120 methylglucose trioleate, and PEG-150 pentaerythritol tetrastearate.

[0097] As an optional implementation, the amount of the thickener added accounts for 0.5% to 5% of the total mass of the fabric cleaning agent, for example, it can be 0.5%, 1%, 2%, 3%, 4%, 5%, etc.

[0098] As an optional implementation, the fabric cleaning agent also includes a pH adjuster.

[0099] As an optional implementation, the pH adjuster is any one or a combination of at least two of citric acid, lactic acid, sodium citrate, and sodium lactate.

[0100] As an optional implementation, the pH of the fabric cleaning agent is 4.5 to 5.5, for example, it can be 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, etc.

[0101] As an optional embodiment, when the fabric cleaning agent is a nonionic surfactant fabric cleaning agent, the fabric cleaning agent comprises, by mass percentage: 0.01%~5% of the Gemini-type quaternary ammonium salt surfactant composition described in the first aspect, 5%~40% of the nonionic surfactant, and the balance being water; and the pH of the fabric cleaning agent is 4.5~5.5.

[0102] As an optional implementation, when the fabric cleaning agent is an anionic surfactant fabric cleaning agent, the fabric cleaning agent comprises, by mass percentage: 0.01%~5% of the Gemini-type quaternary ammonium salt surfactant composition described in the first aspect, 5%~25% of anionic surfactant, and the balance being water; and the pH of the fabric cleaning agent is 4.5~5.5.

[0103] As an optional embodiment, when the fabric cleaning agent is an anionic surfactant fabric cleaning agent, the fabric cleaning agent comprises, by mass percentage: 0.01%~5% of the Gemini-type quaternary ammonium salt surfactant composition described in the first aspect, 5%~25% of anionic surfactant, 1%~25% of amphoteric surfactant, and the balance being water; and the pH of the fabric cleaning agent is 4.5~5.5.

[0104] Fourthly, the present invention provides a method for preparing a fabric cleaning agent with anti-HPV virus activity as described in the third aspect, the preparation method comprising: The Gemini-type quaternary ammonium salt surfactant composition was dissolved in water to obtain the fabric cleaning agent with anti-HPV virus activity.

[0105] As an optional implementation, the preparation method includes: A nonionic surfactant, a Gemini-type quaternary ammonium salt surfactant composition, and a thickener are dissolved in water at 60-80°C, and then a pH adjuster is added to adjust the pH to a slightly acidic state to obtain the fabric cleaning agent with anti-HPV virus activity.

[0106] As an optional implementation, the preparation method includes: An anionic surfactant, amphoteric surfactant, nonionic surfactant, a Gemini-type quaternary ammonium salt surfactant composition and a thickener are dissolved in water at 60-80°C, and then a pH adjuster is added to adjust the pH to weakly acidic to obtain the fabric cleaning agent with anti-HPV virus activity.

[0107] The present invention will be further illustrated by the following examples. Unless otherwise specified, the materials in the examples are prepared according to existing methods or purchased directly from the market.

[0108] Example 1 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method.

[0109] The fabric cleaning agent with anti-HPV virus activity is a non-ionic fabric cleaning agent, and is composed of the following components by mass percentage:

[0110] The Gemini-type quaternary ammonium salt surfactant composition is composed of propylene bis(octadecanoammonium chloride) and propylene bis(octadecyl dimethyl ammonium chloride) in a mass ratio of 2:1; and the pH of the fabric detergent with anti-HPV virus activity is 5.0.

[0111] The preparation method of the fabric cleaning agent with anti-HPV virus effect described in this embodiment includes: The composition of lauryl glucoside, AEO-9, PEG-150 distearate, and the Gemini-type quaternary ammonium salt surfactant was dissolved in water at 70±10℃, and an appropriate amount of citric acid was added to adjust the pH to weakly acidic to obtain the fabric cleaning agent with anti-HPV virus activity.

[0112] Example 2 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method; the only difference from Example 1 is that the mass ratio of propylene bis(octadecylamidopropyl dimethylammonium chloride) and propylene bis(octadecyl dimethylammonium chloride) is 1:1, and the other settings are the same as in Example 1.

[0113] Example 3 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method; the only difference from Example 1 is that the mass ratio of propylene bis(octadecylamidopropyl dimethylammonium chloride) to propylene bis(octadecyl dimethylammonium chloride) is 1:2, and the other settings are the same as in Example 1.

[0114] Example 4 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method; the only difference from Example 1 is that the mass ratio of propylene bis(octadecylamidopropyl dimethylammonium chloride) to propylene bis(octadecyl dimethylammonium chloride) is 3:1, and the other settings are the same as in Example 1.

[0115] Example 5 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method; the only difference from Example 1 is that the amount of the Gemini-type quaternary ammonium salt surfactant composition added is 0.5 wt%, and the other settings are the same as in Example 1.

[0116] Example 6 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method; the only difference from Example 1 is that the amount of the Gemini-type quaternary ammonium salt surfactant composition added is 2 wt%, and the other settings are the same as in Example 1.

[0117] Example 7 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method; the only difference from Example 1 is that propylene bis(octadecanoammonium dimethyl chloride) is replaced with an equal mass of propylene bis(hexadecanoammonium dimethyl chloride); and propylene bis(octadecyl dimethyl chloride) is replaced with an equal mass of propylene bis(hexadecyl dimethyl chloride), and the other settings are the same as in Example 1.

[0118] Example 8 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method; the only difference from Example 1 is that propylene bis(octadecanoammonium dimethyl chloride) is replaced with an equal mass of ethylene bis(octadecanoammonium dimethyl chloride); and propylene bis(octadecyl dimethyl chloride) is replaced with an equal mass of ethylene bis(octadecyl dimethyl chloride), with other settings being the same as in Example 1.

[0119] Example 9 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method.

[0120] The fabric cleaning agent with anti-HPV virus activity is an anionic fabric cleaning agent, and is composed of the following components by mass percentage:

[0121] The Gemini-type quaternary ammonium salt surfactant composition is composed of propylene bis(octadecanoammonium chloride) and propylene bis(octadecyl dimethyl ammonium chloride) in a mass ratio of 2:1; and the pH of the fabric detergent with anti-HPV virus activity is 5.0.

[0122] The preparation method of the fabric cleaning agent with anti-HPV virus effect described in this embodiment includes: The composition of AEC-5Na, CAB-35, AEO-9, PEG-150 distearate and the Gemini-type quaternary ammonium salt surfactant was dissolved in water. At 70±10℃, an appropriate amount of citric acid was added to adjust the pH to weakly acidic, thus obtaining the fabric cleaning agent with anti-HPV virus activity.

[0123] Example 10 This embodiment provides a fabric cleaning agent with anti-HPV virus activity and its preparation method; the only difference from Example 9 is that AEC-5Na is replaced with an equal mass of AES, and the other settings are the same as in Example 9.

[0124] Comparative Example 1 This comparative example provides a fabric cleaning agent, which differs from Example 1 only in that bis(octadecanoammonium chloride) is no longer added, and the content of propylene bis(octadecyl dimethyl ammonium chloride) is made up to 1 wt%, while the other settings are the same as in Example 1.

[0125] Comparative Example 2 This comparative example provides a fabric cleaning agent, which differs from Example 1 only in that propylene bis(octadecyl dimethyl ammonium chloride) is no longer added, but is supplemented to a content of bis(octadecylamidopropyl dimethyl ammonium chloride) to make up to 1 wt%, while other settings are the same as in Example 1.

[0126] Comparative Example 3 This comparative example provides a fabric cleaning agent, which differs from Example 1 only in that the Gemini-type quaternary ammonium salt surfactant composition is replaced with an equal mass of 1 wt% of a composition of benzalkonium chloride and disacyldimethylammonium chloride; wherein the mass ratio of benzalkonium chloride to disacyldimethylammonium chloride is 2:3, and other settings are the same as in Example 1.

[0127] Comparative Example 4 This comparative example provides a fabric cleaning agent, which differs from Example 1 only in that bis(octadecanoammonium dimethyl chloride) is replaced with an equal mass of single-chain octadecanoammonium trimethyl chloride; and propylene bis(octadecanoammonium dimethyl chloride) is replaced with an equal mass of single-chain octadecanoammonium trimethyl chloride, while other settings are the same as in Example 1.

[0128] Comparative Example 5 This comparative example provides a fabric cleaning agent; the only difference from Example 9 is that the Gemini-type quaternary ammonium salt surfactant composition is replaced with an equal mass of 1 wt% of a composition of benzalkonium chloride and disacyldimethylammonium chloride; wherein the mass ratio of benzalkonium chloride to disacyldimethylammonium chloride is 2:3, and other settings are the same as in Example 9.

[0129] Test Example 1 Test samples: Fabric cleaning agents with anti-HPV virus activity provided in Examples 1-8, and fabric cleaning agents provided in Comparative Examples 1-4.

[0130] Test method: (1) Active ingredient content: Specifically determined according to GB / T 13173-2021 7.4.1; (2) Detergent power: Specifically measured according to GB / T 13174-2021; (3) HPV virus inactivation rate: Refer to Section 2.1.1.10 of the "Disinfection Technical Specifications" (2002 edition); details are as follows: (3-1) Equipment: Virus name: HPV pseudovirus (HPV16-GFP); Source: Beijing Yunling Biotechnology; Host cell: 293FT; Neutralizing agent formulation: D / E neutralizes broth; Culture medium names: DMEM / 2902899, FBS / 2582168P; Instruments and equipment: Integrated fluorescence microscopy system light source BZ-X800LE microscope BZ-X810 (WPE-TW0098), carbon dioxide incubator BPN-CRH (WPE-RH0274), inverted microscope LWD300-38LT (WPE-RH0164), biosafety cabinet BSC-1604 II B2 (WPE-RH0313); (3-2) Detection method: Inspection basis: Refer to Section 2.1.1.10 of the "Disinfection Technical Specifications" (2002 edition); Testing environment: temperature 22.6℃, humidity 51%RH; Experimental steps: (a) 293FT cells were seeded in 96-well cell culture plates and allowed to adhere before use; (b) Add 10 μL of virus solution to 90 μL of test substance or 90 μL of PBS, vortex until homogeneous, and allow to react for 5 min. Dilute in 0.9 mL of culture medium to obtain the first concentration, and then perform 10-fold serial dilutions. Add 100 μL of the serially diluted virus solution to the cells and determine the residual virus titer. Set up 4 wells for each concentration gradient and incubate in a CO2 incubator at 37°C. After 72 h of incubation, count the number of fluorescent cells using a fluorescence microscope and calculate the FFU (fluorescence forming units) of the virus.

[0131] (4) Viscosity: Specifically measured according to GB / T 15357-2014, using an NJD-1 type rotary viscometer.

[0132] The specific test results are shown in Table 1 below: Table 1

[0133] As shown in Table 1, fabric detergents containing the nonionic / or anionic surfactant system of the Gemini-type quaternary ammonium salt surfactant composition described in this invention can achieve excellent detergency solely by relying on the surfactant system, without the need to add biological enzymes such as proteases or lipases; at the same time, they also have highly efficient HPV virus inactivation capabilities, and the undiluted solution can significantly reduce the viral load in a short period of time; in addition, they have a suitable viscosity and skin affinity with a weakly acidic pH and viscosity, taking into account stability, user experience and human safety.

[0134] The comparison between Examples 1 and Examples 2-4 shows that the relative content of the two Gemini quaternary ammonium salts is highly sensitive to the HPV inactivation effect, with a clear optimal synergistic range, preferably 1:(1-3), and more preferably 1:2. Deviating from this range, even with completely identical total active ingredient content and basic formulation, the antiviral performance significantly decreases, indicating that its mechanism of action is not a simple addition, but relies on the specific co-assembly of the two types of molecules at the interface. The two molecules must be in an appropriate ratio to form a stable, high-density active microstructure with both physical encapsulation and chemical binding capabilities. This phenomenon confirms the core innovative logic of the present invention—structure determines functional synergy—highlighting its essential advancement over conventional compounding technologies.

[0135] The comparison between Examples 1 and Examples 5-6 shows that at lower addition levels, the virus inactivation rate is significantly insufficient, indicating that the concentration of the active component did not reach the critical interfacial density required for effective intervention of virus particles. However, when the addition level is further increased, the inactivation effect jumps significantly, even reaching extremely high levels, reflecting that the composition has good molecular dispersibility and targeted enrichment ability in a weakly acidic, high-surfactant system, and does not suffer from self-aggregation inactivation or activity shielding due to micelle encapsulation caused by excessive concentration. This non-monotonic response characteristic confirms that its mechanism of action relies on the dynamic active aggregates formed at the fabric-water-virus multiphase interface, rather than the simple concentration dependence of free monomers, which is a key advantage that distinguishes it from traditional quaternary ammonium salt systems that are easily affected by concentration fluctuations. However, although Example 6 improves the antiviral effect, the cost increases significantly; the detergency does not increase with the increase of cationic dosage, indicating that its core role is synergistic rather than replacing the main surfactant, and excessive addition is not beneficial to cleaning performance.

[0136] The comparison between Examples 1 and Examples 7-8 shows that changes in molecular structure affect antiviral performance. When the octadecyl alkyl chain is shortened to a hexadecimal chain, although the basic activity is still maintained, the inactivation effect decreases.

[0137] The comparison between Example 1 and Comparative Examples 1-2 shows that neither component alone can achieve a highly efficient anti-HPV effect, demonstrating the functional complementarity of the two Gemini structures. The alkyl type is mainly responsible for hydrophobic insertion and steric hindrance, while the amide type is responsible for hydrogen bond recognition and hydrophilic targeting. Both must coexist and synergistically assemble in a specific ratio to stably bind to the HPV capsid and induce inactivation in a weakly acidic, highly surfactant environment. This result confirms that the effectiveness of this invention stems from a molecular-level synergistic mechanism, rather than a simple additive effect of components, exhibiting outstanding substantive characteristics and significant progress.

[0138] The comparison between Example 1 and Comparative Example 3 shows that the anti-HPV effect of Comparative Example 3, which uses conventional benzalkonium chloride and dicedyldimethylammonium chloride, is much lower than that of Example 1. This indicates that traditional quaternary ammonium salts cannot effectively inactivate HPV in a weakly acidic, high-surfactant system, highlighting the irreplaceable nature of the Gemini structure synergy of the present invention.

[0139] The comparison between Example 1 and Comparative Example 4 shows that Comparative Example 4, which uses a single-chain quaternary ammonium salt, has extremely low anti-HPV effect, proving that the Gemini-type double quaternary ammonium structure is crucial for virus targeting and interface stability, and that the single-chain structure cannot reproduce the synergistic inactivation mechanism.

[0140] The comparison between Example 1 and Example 9 shows that the anti-HPV effect of Example 1 (non-ionic system) is significantly better than that of Example 9 (anionic system). This may be due to the inhibition of Gemini quaternary ammonium salt by the anionic surfactant. This inhibition should be caused by the strong electrostatic interaction between the anion and the cation. This verifies that the present invention has a better synergistic effect and stronger applicability in non-ionic systems.

[0141] A comparison between Examples 9 and 10 shows that both are anionic fabric cleaning agents, differing only in the type of anionic surfactant (AEC-5Na vs AES). However, their anti-HPV effects differ significantly, indicating a fundamental difference in the degree of inhibition of Gemini quaternary ammonium salts by different anionic surfactants: AEC-5Na, due to its superior compatibility and lower charge density of ether bonds and carboxyl groups, has a weaker shielding effect on cationic active centers; while the strongly negatively charged sulfate groups of AES more easily induce electrostatic neutralization and micellar encapsulation, exacerbating the loss of activity. This comparison confirms the high selectivity of the formulation system in this invention and provides a clear technical direction for the optimization of anionic systems, requiring the matching of low-interference, highly compatible anionic components.

[0142] The comparison between Example 9 and Comparative Example 5 shows that replacing the Gemini combination with conventional quaternary ammonium salt significantly reduces the anti-HPV effect, proving that the Gemini two-component of the present invention still has irreplaceable synergistic antiviral ability in anionic systems, breaking through the technical bottleneck of incompatibility between traditional cationic disinfectants and anionic surfactants.

[0143] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Gemini-type quaternary ammonium salt surfactant composition, characterized in that, Including the Gemini-type quaternary ammonium salts shown in Formula I and the Gemini-type quaternary ammonium salts shown in Formula II; The structural formula of the Gemini-type quaternary ammonium salt represented by Formula I is shown below: Formula I; In Formula I, R1 is selected from any one of C1 to C3 spar groups, R2 is selected from any one of C12 to C18 alkyl groups, and X is selected from halogen atoms; The structural formula of the Gemini-type quaternary ammonium salt represented by Formula II is shown below: Formula II; In Formula II, R1' and R3' are each independently selected from any one of the C1 to C3 spar groups, R2' is selected from any one of the C12 to C18 alkyl groups, and Y is selected from a halogen atom.

2. The Gemini-type quaternary ammonium salt surfactant composition according to claim 1, characterized in that, In Formula I, R1 is selected from ethylene-based or propylene-based, preferably propylene-based; Preferably, in Formula I, R2 is selected from C16-C18 alkyl groups, and more preferably C18 alkyl groups; Preferably, in Formula I, the halogen atom is selected from any one of F, Cl, Br, and I, with Cl being the most preferred.

3. The Gemini-type quaternary ammonium salt surfactant composition according to claim 1, characterized in that, In Formula II, R1' is selected from ethylene-based or propylene-based, preferably propylene-based; Preferably, in Formula II, R3' is selected from ethylene-based or propylene-based, and more preferably propylene-based; Preferably, in Formula II, R2' is selected from C16-C18 alkyl groups, and more preferably C18 alkyl groups; Preferably, in Formula II, the halogen atom is selected from any one of F, Cl, Br, and I, with Cl being the most preferred.

4. The Gemini-type quaternary ammonium salt surfactant composition according to claim 1, characterized in that, The Gemini-type quaternary ammonium salt surfactant composition consists of propylene bis(octadecyl dimethyl ammonium chloride) and propylene bis(octadecamidopropyl dimethyl ammonium chloride).

5. The Gemini-type quaternary ammonium salt surfactant composition according to claim 1, characterized in that, The mass ratio of the Gemini-type quaternary ammonium salt shown in Formula I to the Gemini-type quaternary ammonium salt shown in Formula II is 1:(0.5~5), preferably 1:(1~3), and more preferably 1:

2.

6. The use of a Gemini-type quaternary ammonium salt surfactant composition according to any one of claims 1 to 5 in the preparation of a cleaning product for anti-HPV virus.

7. A fabric cleaning agent with anti-HPV virus activity, characterized in that, The fabric cleaning agent with anti-HPV virus activity includes the Gemini-type quaternary ammonium salt surfactant composition as described in any one of claims 1 to 5.

8. The fabric cleaning agent with anti-HPV virus activity according to claim 7, characterized in that, The amount of the Gemini-type quaternary ammonium salt surfactant composition added accounts for 0.01% to 5% of the total mass of the fabric cleaning agent, preferably 0.5% to 2%, and more preferably 1%.

9. The fabric cleaning agent with anti-HPV virus activity according to claim 7, characterized in that, The fabric cleaning agent also includes any one or a combination of at least two of the following: nonionic surfactants, anionic surfactants, and amphoteric surfactants. Preferably, the nonionic surfactant includes any one or a combination of at least two of alkyl glycosides, fatty alcohol polyoxyethylene ethers, and alkanolamides; Preferably, the amount of the nonionic surfactant added accounts for 5% to 40% of the total mass of the fabric cleaning agent; Preferably, the anionic surfactant includes any one or a combination of at least two of alkyl ether carboxylates and alkyl ether sulfates; Preferably, the amount of the anionic surfactant added accounts for 5% to 25% of the total mass of the fabric cleaning agent; Preferably, the zwitterionic surfactant includes any one or a combination of at least two of cocamidopropyl dimethyl betaine and alkylamidopropyl amine oxides; Preferably, the amount of the zwitterionic surfactant added accounts for 1% to 25% of the total mass of the fabric cleaning agent; Preferably, the fabric cleaning agent further includes a thickener; Preferably, the thickener comprises any one or a combination of at least two of PEG-150 distearate, PEG-120 methyl glucoside dioleate, PEG-120 methyl glucoside trioleate, and PEG-150 pentaerythritol tetrastearate. Preferably, the amount of the thickener added is 0.5% to 5% of the total mass of the fabric cleaning agent; Preferably, the fabric cleaning agent further includes a pH adjuster; Preferably, the pH adjuster is any one or a combination of at least two of citric acid, lactic acid, sodium citrate, and sodium lactate; Preferably, the pH of the fabric cleaning agent is 4.5 to 5.

5.

10. A method for preparing a fabric cleaning agent with anti-HPV virus activity according to any one of claims 7 to 9, characterized in that, The preparation method includes: The Gemini-type quaternary ammonium salt surfactant composition was dissolved in water to obtain the fabric cleaning agent with anti-HPV virus activity. Alternatively, the preparation method includes: A nonionic surfactant, a Gemini-type quaternary ammonium salt surfactant composition and a thickener are dissolved in water at 60-80°C, and then a pH adjuster is added to adjust the pH to weakly acidic to obtain the fabric cleaning agent with anti-HPV virus activity. Alternatively, the preparation method includes: An anionic surfactant, amphoteric surfactant, nonionic surfactant, a Gemini-type quaternary ammonium salt surfactant composition and a thickener are dissolved in water at 60-80°C, and then a pH adjuster is added to adjust the pH to weakly acidic to obtain the fabric cleaning agent with anti-HPV virus activity.

Images