Moisturizing, soothing and anti-allergy composition as well as preparation method and application thereof

Abstract

The invention provides a moisturizing, soothing and anti-allergy composition as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The composition comprises the following raw materials: a gentiana macrophylla extract, a veronica polita extract, a polygonatum odoratum extract, a cynanchum atratum extract and a solvent. The preparation method of the gentiana macrophylla extract comprises the following steps: crushing gentiana macrophylla roots, mixing the crushed gentiana macrophylla roots with water, and carrying out enzymolysis, alcohol extraction and purification, thereby obtaining the gentiana macrophylla extract. The preparation method of the veronica didyma extract comprises the following steps: crushing the medicinal veronica didyma whole herb, carrying out gradient alcohol extraction, combining extracting solutions, and drying to obtain the veronica didyma extract. The skin care composition provided by the invention takes natural extracts, namely the gentiana macrophylla extract, the veronica polita extract, the polygonatum odoratum extract and the cynanchum atratum extract, as main active ingredients, and all the components are in synergistic cooperation, so that the skin care composition has excellent moisturizing, soothing and anti-allergy effects, can improve dry and sensitive states of skin, and remarkably improves a skin barrier function.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a moisturizing, soothing, and anti-allergic composition, its preparation method, and its application. Background Technology

[0002] External factors such as ultraviolet radiation, environmental pollution, and even seasonal changes, as well as internal factors such as mental and psychological stress, all contribute to increasingly fragile skin. Therefore, anti-allergy ingredients have become indispensable in cosmetic formulations. Meanwhile, in the cosmetics industry, with the concept of precision skincare gaining popularity, sensitive skin can be further categorized into dry sensitive skin, normal sensitive skin, and oily sensitive skin. Different skin types require different targeted solutions. Addressing common skin problems requires focusing resources effectively to achieve precise and efficient solutions for sensitive skin issues. Existing technologies for sensitive skin solutions primarily address four mechanisms: impaired skin barrier function, epidermal inflammation, abnormal skin nervous system, and microecological imbalance. Their characteristic is comprehensive coverage of all pathways. However, existing technologies suffer from insufficient precision, failing to meet the current market demand for more precise and efficient skincare. Furthermore, the contribution of each pathway to addressing different skin types varies. For example, individuals with dry, sensitive skin are characterized by year-round dryness, a damaged skin barrier, persistent micro-inflammatory reactions, and are prone to peeling, itching, redness, and stinging. Their approach should focus on addressing skin barrier issues through moisturizing and hydration, supplemented by anti-inflammatory measures to eliminate adverse reactions, reduce micro-inflammatory levels, create a healthy inner skin environment, and enhance the skin's own anti-sensitivity capabilities.

[0003] Chinese patent application CN112206181A discloses a moisturizing composition. By weight, the active components of this moisturizing composition include: 0.02-0.08 parts of high-molecular-weight sodium hyaluronate; 0.2-0.8 parts of a saccharide isomer; 0.5-2 parts of biosaccharide gum-1; 0.3-0.7 parts of panthenol; and 1-3 parts of PEG / PPG-17 / 6 copolymer. This invention selects high-molecular-weight sodium hyaluronate, saccharide isomer, biosaccharide gum-1, panthenol, and PEG / PPG-17 / 6 copolymer as raw materials. The various active components work together to provide a synergistic moisturizing effect on the skin. Chinese patent application CN119950384A discloses a soothing composition and its use, wherein the soothing composition comprises Scutellaria baicalensis root extract, Ophiopogon japonicus root extract, and Cornus officinalis extract, or Scutellaria baicalensis root extract, Ophiopogon japonicus root extract, and Tribulus terrestris fruit extract, or Scutellaria baicalensis root extract, Ophiopogon japonicus root extract, Cornus officinalis extract, and Tribulus terrestris fruit extract; the combination of the above three or four plant extracts can synergistically enhance the soothing effect of the soothing composition. Chinese patent application CN110833581A discloses an anti-allergic composition, which, by weight, comprises 1-5 parts of Opuntia ficus-indica stem extract, 1-5 parts of Houttuynia cordata extract, 1-5 parts of Chamomile extract, 1-5 parts of Paeonia suffruticosa root bark extract, 1-5 parts of Portulaca oleracea extract, 1-5 parts of Scutellaria baicalensis root extract, and 0.1-2 parts of ectoine. However, most of the aforementioned patents or currently available skincare products suffer from problems such as insignificant effects, limited efficacy, and adverse reactions due to excessive use of a single ingredient.

[0004] Therefore, there is an urgent need to find a product with multiple functions, safety, no adverse reactions, and clear effects that moisturizes and soothes the skin. Developing more precise and efficient anti-allergic, moisturizing, and soothing compositions and applying them to cosmetics is a pressing need with broad market prospects. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides a moisturizing, soothing, and anti-allergic composition, its preparation method, and its application. By combining natural plant extracts, it achieves a synergistic improvement in moisturizing, soothing, and anti-allergic effects.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] On one hand, the present invention provides a moisturizing, soothing, and anti-allergic composition comprising the following raw materials: Gentiana macrophylla extract, Veronica persica extract, Polygonatum odoratum extract, Cynanchum paniculatum extract, and solvent; the preparation method of the Gentiana macrophylla extract is as follows: Gentiana macrophylla root is pulverized and mixed with water, enzymatically hydrolyzed, extracted with alcohol, and purified to obtain the extract; the preparation method of the Veronica persica extract is as follows: the whole herb of medicinal Veronica persica is pulverized, subjected to gradient alcohol extraction, the extracts are combined, and dried to obtain the extract.

[0008] Preferably, the solvent is selected from at least one of butanediol, 1,2-hexanediol, 1,2-pentanediol, water, glycerol, and propylene glycol.

[0009] More preferably, the solvent is butanediol, 1,2-hexanediol, 1,2-pentanediol, and water.

[0010] Preferably, the mass ratio of butanediol, 1,2-hexanediol, 1,2-pentanediol and water is 25-32:1-2:0.1-0.8:60-65.

[0011] Any point value or any subrange value within the range of 25-32:1-2:0.1-0.8:60-65 is applicable to this invention, including but not limited to 25:2:0.1:65, 32:1:0.8:60, 30:1.5:0.5:63, 26:1.1:0.2:61, 27:1.2:0.3:62, 28:1.3:0.4:63, 29:1.4:0.5:64, and 30:1.5:0.6:65.

[0012] More preferably, the mass ratio of butanediol, 1,2-hexanediol, 1,2-pentanediol and water is 30:1.5:0.5:63.

[0013] Preferably, the preparation method of the Gentiana macrophylla extract is as follows: Gentiana macrophylla root is pulverized and mixed with water, cellulase, papain and polygalacturonase are added for enzymatic hydrolysis, and then 70wt%-75wt% ethanol aqueous solution is added to the enzymatically hydrolyzed system for reflux extraction to obtain crude extract; the crude extract is enriched and purified by D101 macroporous resin column, dried, and then obtained.

[0014] More preferably, the preparation method of the Gentiana macrophylla extract is as follows: Gentiana macrophylla root is washed, dried, pulverized, and passed through an 80-mesh sieve. Water is added at a material-to-liquid ratio of 1g:10-20mL, and the pH of the system is adjusted to 4.5-5.0 using a citrate-sodium citrate buffer solution. The temperature is 40-55℃. Cellulase, papain, and polygalacturonase are added at a mass ratio of 1-3:0.5-0.9:1 for enzymatic hydrolysis. Then, 70wt%-75wt% of the extract is added to the hydrolyzed system. Extract the crude extract three times by reflux with t% ethanol aqueous solution, combine the extracts to obtain a crude extract; purify the crude extract by enrichment using a D101 macroporous resin column at a loading rate of 1-3 BV / h; after loading, elute with deionized water at a rate of 2-4 BV / h to remove impurities until the eluent is colorless and transparent; then elute with 55-65% (v / v) ethanol solution at a rate of 1-3 BV / h, collect the eluent, concentrate under reduced pressure to a relative density of 1.10-1.15 (60℃), and dry to obtain the final product.

[0015] Preferably, the preparation method of the Veronica persica extract is as follows: the whole herb of Veronica persica is pulverized, and extracted once for 10-30 min at 10-20℃ with 40wt%-50wt% ethanol solution at a material-to-liquid ratio of 1g:10-20mL; then ethanol is added to the final solution with an ethanol concentration of 60wt%-65wt% for a second extraction for 20-40 min; finally, ethanol is added to the final solution with an ethanol concentration of 70wt%-80wt% for a second extraction for 10-30 min, the extracts are combined, dried, and the extract is obtained.

[0016] More preferably, the preparation method of the Veronica persica extract is as follows: the dried whole herb of Veronica persica is pulverized, and extracted once for 20 min at 15℃ with 45wt% ethanol solution at a material-to-liquid ratio of 1g:150mL; then ethanol is added to the final solution with an ethanol concentration of 62wt% for a second extraction of 30 min; finally, ethanol is added to the final solution with an ethanol concentration of 75wt% for a second extraction of 25 min, the extracts are combined, and dried to obtain the extract.

[0017] Preferably, the mass ratio of the Gentiana macrophylla extract, Veronica persica extract, Polygonatum odoratum extract and Cynanchum paniculatum extract is 1-3:0.5-1.2:2-3:0.1-0.8.

[0018] Any point value or any subrange value within the range of 1-3:0.5-1.2:2-3:0.1-0.8 is applicable to this invention, including but not limited to 1:1.2:2:0.8, 3:0.5:3:0.1, 2:1:2.5:0.5, 2:1.2:2:0.8, 2:0.5:3:0.1, 1:0.6:2.1:0.2, 1:0.7:2.2:0.3, 1:0.8:2.3:0.4, 1:0.9:2.4:0.5, 1:1:2.5:0.6, 1:1.1:2.6:0.7, and 1:1.2:2.7:0.8.

[0019] More preferably, the mass ratio of the Gentiana macrophylla extract, Veronica persica extract, Polygonatum odoratum extract and Cynanchum paniculatum extract is 2:1:2.5:0.5.

[0020] Preferably, the mass of the solvent is 75%-85% of the total mass of the raw materials in the composition.

[0021] In a second aspect, the present invention provides a method for preparing the composition, comprising the following steps: mixing Gentiana macrophylla extract, Veronica persica extract, Polygonatum odoratum extract, Cynanchum paniculatum extract and solvent.

[0022] Thirdly, the present invention provides the use of the described composition in the preparation of cosmetics.

[0023] Preferably, the cosmetics include toners, serums, lotions, creams, gels, masks, eye creams, neck creams, hand masks, foot masks, or makeup removers.

[0024] Compared with the prior art, the present invention has the following beneficial effects: 1. This invention scientifically combines extracts of Gentiana macrophylla, Veronica persica, Polygonatum odoratum, and Cynanchum paniculatum. These four extracts work synergistically and complement each other, exhibiting significant skincare effects in moisturizing, soothing, and anti-allergy. The composition deeply moisturizes the skin, increases the moisture content of the stratum corneum, improves dryness, roughness, and flaking, enhances the skin's own water-locking ability, and maintains the skin's hydrated state. Simultaneously, it effectively soothes discomfort symptoms such as redness, burning, stinging, and itching caused by external environmental stimuli, physical irritation, or barrier damage, inhibits skin sensitivity reactions, reduces skin sensitivity, enhances skin tolerance, and repairs and strengthens the skin barrier function. Compared with single extracts, the compound composition of this invention significantly improves moisturizing performance, soothing effects, and anti-allergy capabilities. It is gentle, safe, and stable, suitable for daily care and repair of dry skin, sensitive skin, and skin with damaged barriers.

[0025] 2. This invention employs a specific preparation process to extract and purify Gentiana macrophylla extract, which efficiently enriches the active ingredients with moisturizing, soothing, and anti-allergic activities, effectively enhancing the extract's potency and stability. Simultaneously, by controlling the specific mass ratio of Gentiana macrophylla extract, Veronica persica extract, Polygonatum odoratum extract, and Cynanchum paniculatum extract in a compound, the four components exhibit a significant synergistic effect, further strengthening the composition's moisturizing and water-locking ability, soothing and repairing ability, and anti-allergic and soothing effects on the skin. Compared to compositions using conventional extraction methods or arbitrary ratios, this invention demonstrates more outstanding and unexpected technical effects in improving dry skin, relieving skin sensitivity and discomfort, reducing skin redness and stinging, and repairing the skin barrier. Detailed Implementation

[0026] To make the technical means, creative features, achieved objectives, and effects of this invention readily understandable, the invention is further illustrated below with specific embodiments. However, these embodiments are merely preferred embodiments and not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments described herein without creative effort are all within the scope of protection of this invention. It is worth noting that the raw materials used in this invention are all common commercially available products, and their sources are not specifically limited. The technical and scientific terms used in the embodiments have the meanings commonly understood by those skilled in the art to which this invention pertains.

[0027] Polygonatum odoratum extract: purchased from Shaanxi Huaze Biotechnology Co., Ltd., product number HZ56; Cynanchi Atrati extract: purchased from Xi'an Haoze Biotechnology Co., Ltd., specification 10:1.

[0028] The preparation method of Gentiana macrophylla extract is as follows: Gentiana macrophylla root is washed, dried, pulverized, and passed through an 80-mesh sieve. Water is added at a material-to-liquid ratio of 1g:15mL and mixed. The pH of the system is adjusted to 5.0 using a citrate-sodium citrate buffer solution at 50℃. Cellulase, papain, and polygalacturonase at a mass ratio of 2:0.6:1 are added for enzymatic hydrolysis. Then, 72wt% ethanol aqueous solution is added to the hydrolyzed system and refluxed three times. The extracts are combined to obtain a crude extract. The crude extract is enriched and purified by a D101 macroporous resin column at a loading rate of 2BV / h. After loading, deionized water is eluted at a rate of 3BV / h to remove impurities until the eluent is colorless and transparent. Then, 60% (v / v) ethanol solution is eluted at a rate of 2BV / h. The eluent is collected, concentrated under reduced pressure to a relative density of 1.10-1.15 (60℃), and dried to obtain Gentiana macrophylla extract, which is used in the embodiments of the present invention.

[0029] The preparation method of Veronica persica extract is as follows: the dried whole herb of Veronica persica is pulverized, passed through an 80-mesh sieve, and extracted once for 20 min at 15℃ with 45wt% ethanol solution at a material-to-liquid ratio of 1g:150mL; then ethanol is added to the final solution with an ethanol concentration of 62wt% for a second extraction of 30 min; finally, ethanol is added to the final solution with an ethanol concentration of 75wt% for a second extraction of 25 min, the extracts are combined, dried, and the Veronica persica extract is obtained, which is used in the embodiments of the present invention.

[0030] Example 1 A moisturizing, soothing, and anti-allergic composition, with the raw material composition shown in Table 1 below by weight: Table 1

[0031] Preparation method: Mix the above raw materials evenly.

[0032] Example 2 A moisturizing, soothing, and anti-allergic composition, with the raw material composition shown in Table 2 below by weight: Table 2

[0033] Preparation method: Mix the above raw materials evenly.

[0034] Example 3 A moisturizing, soothing, and anti-allergic composition, with the raw material composition shown in Table 3 below by weight: Table 3

[0035] Preparation method: Mix the above raw materials evenly.

[0036] Comparative Example 1 A moisturizing, soothing, and anti-allergic composition, compared with Example 1, only differs in the amount of raw materials. The composition of the raw materials is shown in Table 4 below: Table 4

[0037] Preparation method: Mix the above raw materials evenly.

[0038] Comparative Example 2 A moisturizing, soothing, and anti-allergic composition, compared with Example 1, only differs in the preparation method of Gentiana macrophylla extract: Gentiana macrophylla root is washed, dried, pulverized, and passed through an 80-mesh sieve. Then, it is extracted three times by reflux with 72wt% ethanol aqueous solution at a material-liquid ratio of 1g:15mL. The extracts are combined, concentrated under reduced pressure to a relative density of 1.10-1.15 (60℃), and dried to obtain Gentiana macrophylla extract.

[0039] The rest is the same as in Example 1.

[0040] Comparative Example 3 A moisturizing, soothing, and anti-allergic composition, compared to Example 1, differs only in the preparation method of the Veronica persica extract: The dried whole herb of medicinal Veronica was pulverized, passed through an 80-mesh sieve, and extracted at 15°C with 75wt% ethanol solution at a material-to-liquid ratio of 1g:150mL for 30 minutes. This extraction was repeated three times. The extracts were combined and dried to obtain the Veronica extract. The rest of the process was the same as in Example 1.

[0041] Comparative Example 4 A moisturizing, soothing, and anti-allergic composition, which is identical to Example 1 except that the Gentiana macrophylla extract is replaced with Astragalus membranaceus extract.

[0042] Test Example 1 Human patch test Experimental Procedure: A total of 30 subjects were randomly selected based on their gender and age composition (meeting the inclusion and exclusion criteria of the "Diagnostic Criteria and Treatment Principles for Cosmetic Contact Dermatitis"). Qualified patch materials were used. 0.05 mL of the compositions prepared in Examples 1-3 and Comparative Examples 1-4 was added to the patch applicator as the sample group. The control well served as the blank control (without any substance). Both the sample group and the blank control were applied to the flexor side of the forearm of each subject (each subject had 8 application points, corresponding to the compositions prepared in Examples 1-3, Comparative Examples 1-4, and the blank control, respectively). The patches were gently pressed with the palm of the hand to ensure even application to the skin, and left for 24 hours. After removing the patch applicator, a 30-minute interval was observed until the pressure marks disappeared. Skin reactions were observed again at 24 hours and 48 hours after the patch test.

[0043] Judgment criteria: According to the "Diagnostic Criteria and Treatment Principles for Contact Dermatitis Caused by Cosmetics", the following classifications are used: no reaction at the test site (Grade 0), light red spots on the skin (Grade 1), erythema, infiltration or papules on the skin (Grade 2), edematous erythema and papules on the skin (Grade 3), and significant redness and swelling on the skin with papules or bullae (Grade 4).

[0044] If more than two out of 30 cases experience grade 2 adverse skin reactions, or if any one case experiences a grade 3 or higher adverse skin reaction, the test substance is deemed to have an adverse effect on the human body.

[0045] The specific results are shown in Table 5 below: Table 5

[0046] The results show that the compositions prepared in Examples 1-3 and Comparative Examples 1-4 of this invention have no adverse reactions on the human body.

[0047] Test Example 2 In vitro gravimetric method for moisturizing performance testing Weigh 0.2g of the compositions prepared in Examples 1-3 and Comparative Examples 1-4 respectively, and apply them evenly to a 5cm×5cm glass plate with microporous ventilation tape attached. Place the glass plate in a desiccator at a constant temperature (25℃), weigh the glass plate after 2h and 4h respectively, and calculate its moisture retention rate.

[0048] The formula for calculating the moisturizing rate is: Moisturizing rate % = (M2-M0) / (M1-M0)×100%.

[0049] Where M0 is the mass of the glass plate in g, M1 is the mass of the glass plate after sample addition in g, and M2 is the mass of the glass plate after being placed in the desiccator for a period of time in g.

[0050] The specific test results are shown in Table 6: Table 6

[0051] The results show that the compositions prepared in Examples 1-3 of this invention have better moisturizing properties compared with Comparative Examples 1-4.

[0052] Test Example 3 External soothing and anti-allergic effects 1. Experimental Principle Hyaluronic acid is a component of the tissue matrix that restricts the diffusion of water and other extracellular substances. When hydrolyzed by hyaluronidase, it makes the intercellular spaces non-adhesive, causing cell degranulation and the release of newly synthesized mediators, exerting biological effects and leading to immediate-type hypersensitivity reactions. Hyaluronidase is a participant in allergic reactions and is strongly correlated with allergies; therefore, the inhibitory effect of a test substance on hyaluronidase is often used to reflect its effect on alleviating and improving type I hypersensitivity reactions.

[0053] 2. Reagent preparation (1) Hyaluronidase: Prepare 500 μ / mL hyaluronidase using acetate buffer solution as solvent. It should be prepared and used immediately and should not be left overnight.

[0054] (2) Potassium hyaluronic acid: Prepare potassium hyaluronic acid with a concentration of 0.5 mg / mL using acetic acid buffer solution as solvent.

[0055] (3) Buffer solution: 4.8 mL of solution A (0.2 mol / L acetic acid, i.e., 11.55 mL of glacial acetic acid dissolved in 1000 mL of distilled water) and 45.2 mL of solution B (0.2 mol / L sodium acetate, i.e., 16.4 g of CH3COONa or 27.2 g of CH3COONa·3H2O dissolved in 1000 mL of distilled water) are mixed and diluted to 100 mL to prepare an acetate buffer solution with pH = 5.6.

[0056] (4) Acetic acid acetone solution: Mix 50 mL of 1.0 mol / L sodium carbonate solution and 3.5 mL of acetylacetone solution evenly (prepare fresh before use).

[0057] (5) Preparation of p-dimethylaminobenzaldehyde (P-DAB) colorimetric reagent: Mix 0.8g of p-dimethylaminobenzaldehyde with 15mL of HCl and anhydrous ethanol evenly before use.

[0058] (6) CaCl2 solution: 2.5 mol / L.

[0059] (7) NaOH solution: 5 mol / L.

[0060] 3. Experimental Procedure: Take 0.1 mL of CaCl2 solution (0.25 mmol / L) and 0.5 mL of hyaluronidase solution (100 μ / mL) and react in a 37℃ water bath for 20 min; add 0.5 mL of sample solution (the compositions prepared in Examples 1-3 and Comparative Examples 1-4), and incubate for 20 min; then add 0.5 mL of sodium hyaluronate solution (0.5 g / L), react for 30 min, and then remove and cool for 5 min; add 0.1 mL of sodium hydroxide solution (0.4 mol / L) and 0.5 mL of sodium hydroxide solution (0.4 mol / L) and 0.5 mL of sodium hydroxide solution (0.4 mol / L) and 0.5 mL of sodium hydroxide solution (0.5 g / L) and 0.5 mL of sodium hydroxide solution (0.4 mol / L) and 0.5 mL of sodium hydroxide solution (0.5 g / L) and 0.5 mL of sodium hydroxide solution (0.4 mol / L) and 0.5 mL of sodium hydroxide solution (0.5 g / L) and 0.5 mL of sodium hydroxide solution (0.25 mmol ... then react in a 37℃ water bath for 20 min. Incubate L of acetylacetone solution (3.5 mL acetylacetone dissolved in 50 mL of 1.0 mol / L sodium carbonate solution) in a boiling water bath for 15 min, then immediately transfer to an ice water bath for 5 min. Add 1 mL of Ehrlich's reagent (0.8 g p-dimethylaminobenzaldehyde dissolved in 15 mL concentrated hydrochloric acid and 15 mL anhydrous ethanol), and dilute with 3 mL of anhydrous ethanol. Incubate at room temperature for 20 min for color development, and measure the absorbance at 540 nm. Use 5% dipotassium glycyrrhizate solution as a positive control. Calculate the inhibition rate against hyaluronidase using the following formula: Hyaluronidase inhibition rate = {[(AB)-(CD)] / (AB)}×100%.

[0061] Where: A - absorbance of the control solution (using acetate buffer solution instead of the sample solution); B - absorbance of the control blank solution (using acetate buffer solution instead of the sample solution and enzyme solution); C - absorbance of the sample solution; D - absorbance of the sample blank solution (using acetate buffer solution instead of the enzyme solution).

[0062] The specific test results are shown in Table 7 below: Table 7

[0063] The results show that the compositions prepared in Examples 1-3 of this invention have better soothing and anti-allergic properties compared with Comparative Examples 1-4.

[0064] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A moisturizing, soothing, and anti-allergic composition, characterized in that, The product includes the following raw materials: Gentiana macrophylla extract, Veronica persica extract, Polygonatum odoratum extract, Cynanchum paniculatum extract, and solvent; the preparation method of the Gentiana macrophylla extract is as follows: Gentiana macrophylla root is pulverized, mixed with water, enzymatically hydrolyzed, extracted with alcohol, and purified to obtain the product; the preparation method of the Veronica persica extract is as follows: the whole herb of medicinal Veronica persica is pulverized, subjected to gradient alcohol extraction, the extracts are combined, and dried to obtain the product.

2. The composition according to claim 1, characterized in that, The solvent is selected from at least one of butanediol, 1,2-hexanediol, 1,2-pentanediol, water, glycerol, and propylene glycol.

3. The composition according to claim 2, characterized in that, The solvent is butanediol, 1,2-hexanediol, 1,2-pentanediol and water.

4. The composition according to claim 3, characterized in that, The mass ratio of butanediol, 1,2-hexanediol, 1,2-pentanediol and water is 25-32:1-2:0.1-0.8:60-65.

5. The composition according to claim 1, characterized in that, The preparation method of the Gentiana macrophylla extract is as follows: Gentiana macrophylla root is crushed and mixed with water, and cellulase, papain and polygalacturonase are added for enzymatic hydrolysis. Then, 70wt%-75wt% ethanol aqueous solution is added to the enzymatic hydrolysis system and refluxed for extraction to obtain crude extract. The crude extract is enriched and purified by D101 macroporous resin column and dried to obtain the final product.

6. The composition according to claim 1, characterized in that, The preparation method of the Veronica persica extract is as follows: the whole herb of Veronica persica is pulverized, and extracted once for 10-30 min at 10-20℃ with 40wt%-50wt% ethanol solution at a material-to-liquid ratio of 1g:10-20mL; then ethanol is added to the final solution with an ethanol concentration of 60wt%-65wt% for a second extraction for 20-40 min; finally, ethanol is added to the final solution with an ethanol concentration of 70wt%-80wt% for a second extraction for 10-30 min, the extracts are combined, dried, and the extract is obtained.

7. The composition according to claim 1, characterized in that, The mass ratio of the extracts of Gentiana macrophylla, Veronica persica, Polygonatum odoratum, and Cynanchum paniculatum is 1-3:0.5-1.2:2-3:0.1-0.

8.

8. The composition according to claim 1, characterized in that, The mass of the solvent is 75%-85% of the total mass of the raw materials in the composition.

9. A method for preparing the composition according to any one of claims 1-8, characterized in that, Includes the following steps: Gentian root extract, Veronica root extract, Polygonatum root extract, Cynanchum root extract and solvent were mixed.

10. Use of the composition according to any one of claims 1-8 in the preparation of cosmetics.

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