Fluorescence detection module

Abstract

The invention relates to a fluorescence detection module which comprises a rotating mechanism, an optical filter turntable, a light source assembly, an optical signal acquisition assembly and an optical fiber, the light source assembly is used for emitting excitation fluorescence to the sample cell; the rotating mechanism comprises a central shaft and a driving motor, the optical filter turntable is rotatably connected to the central shaft, and a plurality of optical filters with different filtering wave bands are arranged on the optical filter turntable; the optical fiber is connected among the light source assembly, the sample pool and the light signal acquisition assembly and used for conducting fluorescence signals; the driving motor drives the central shaft to rotate and drives the optical filter turntable to synchronously rotate, so that excitation fluorescence emitted by the light source assembly and / or receiving fluorescence passing through the sample pool are / is received by the optical signal acquisition assembly after passing through the optical filters with different filtering wavelengths. According to the invention, a standardized rapid detection structure can be formed, the modules can be stacked and combined according to market demands, a diversified complete machine is formed for use, and the detection speed and the detection efficiency are improved.

Description

Technical Field

[0001] This application relates to the technical fields of biology, environmental science, food safety, and immunofluorescence, and specifically to a fluorescence detection module. Background Technology

[0002] Fluorescence is a photoluminescence phenomenon. Fluorescence refers to the phenomenon that certain chemical substances emit light after absorbing external light. Microscopically, it is the process by which ground-state electrons absorb photons, transition to an excited state, and then return to the ground state to release photons.

[0003] During detection, the sample is irradiated with excitation light of a specific wavelength. The induced fluorescent substances absorb the energy of the excitation light and transition to an excited state. Then, during the process of returning from the excited state to the ground state, they release fluorescence with a longer wavelength. By detecting parameters such as fluorescence intensity, wavelength, polarization state, and lifetime, information about the presence, concentration, activity, and biological processes of biomolecules can be obtained.

[0004] Fluorescence detection technology is a major detection method in the field of sensing, characterized by its speed, simplicity, convenience, and high sensitivity. It typically requires labeling target molecules with fluorescent dyes, and then performing qualitative or quantitative analysis of the labeled substances by detecting their fluorescence properties. Organic dye-based fluorescence detection methods are simple, diverse, real-time, and non-destructive. Many organic dyes, fluorescent proteins, and luminescent metal complexes have been used in fluorescence detection, promoting advancements in optical sensing, optical imaging, and other fields.

[0005] Excitation source: Use ultraviolet or visible light as the excitation source, with a wavelength range typically between 300 nm and 700 nm, depending on the absorption spectrum of the target fluorescent molecule.

[0006] Fluorescence emission: When fluorescent molecules in a biological sample are irradiated by an excitation light source, they absorb the energy of photons and transition from the ground state to an excited state. Then, these molecules release some energy through non-radiative pathways (such as vibration), returning to a lower energy state. When they finally return to the ground state, they emit photons of a longer wavelength, i.e., fluorescence.

[0007] Fluorescence collection and detection: Fluorescence signals are collected using optical systems (such as microscopes, optical fibers, lenses, etc.), and filters are used to remove excitation light and other non-fluorescent background, allowing only fluorescence within a specific wavelength range to pass through. Finally, the fluorescence signals are received by photodetectors (such as photomultiplier tubes, CCD cameras) and converted into electrical signals.

[0008] Fluorescence detection technology has been widely used in medical, scientific research and industrial applications, such as clinical disease diagnosis, animal disease detection, food safety and quantitative molecular biology research related to medicine, agriculture and animal husbandry, and biology. It is also widely used in gene expression research, transgenic research, drug efficacy evaluation, pathogen detection and many other fields.

[0009] Currently, multi-channel optical detection systems are commonly used to detect the fluorescence signal intensity of a sample cell. Specifically, the sample cell is illuminated with light of a specific wavelength, and optical detection methods such as photodetectors are used to detect information such as the fluorescence signal intensity and distribution pattern of the sample cell, thereby enabling qualitative and quantitative analysis of the sample in the sample cell.

[0010] Currently, related fluorescence detection structures and instruments are slow and inefficient, and the integration of related products in the market is not ideal in terms of generalization, serialization, and combination. Summary of the Invention

[0011] The purpose of this application is to provide a fluorescence detection module that can form a standardized and rapid detection structure. The number of modules can be stacked and combined according to market demand to form a variety of complete machines for use, thereby increasing detection speed and efficiency.

[0012] To achieve the above objectives, the present invention provides a fluorescence detection module, including a rotating mechanism, a filter turntable, a light source assembly, a light signal acquisition assembly, and an optical fiber; The light source assembly is used to emit excitation fluorescence into the sample cell; The rotating mechanism includes a central shaft and a drive motor. The filter turntable is rotatably connected to the central shaft, and the filter turntable is provided with multiple filters of different filtering bands. The optical fiber connects the light source assembly, the sample cell, and the optical signal acquisition assembly to conduct fluorescence signals. The drive motor drives the central shaft to rotate, which in turn drives the filter turntable to rotate synchronously. This is used to ensure that the excitation fluorescence emitted by the light source assembly and / or the received fluorescence after passing through the sample cell are received by the optical signal acquisition assembly after passing through filters of different wavelengths.

[0013] In an optional embodiment, the filter turntable includes at least two, and the two filter turntables are mounted at a distance from each other on the central axis; The two filter turntables each include an excitation filter turntable and a receiving filter turntable. The optical fiber is connected between the excitation filter turntable, the sample cell, and the receiving filter turntable, so that the laser fluorescence and the received fluorescence can both pass through filters of different filtering bands.

[0014] In an optional embodiment, filter covers are respectively arranged in an overlapping manner on both sides of the excitation filter turntable and on both sides of the receiving filter turntable, and the filters are respectively connected between the filter covers and the filter turntable. The filters on each of the filter turntables are axially aligned along the central axis, and the filters on the excitation filter turntable and the receiving filter turntable are axially aligned along the central axis.

[0015] In an optional embodiment, lenses are fixedly arranged on both sides of the filter turntable, and the light source assembly includes multiple LEDs. The number of lenses, LEDs and filters are corresponding to each other, and in the initial state, the positions of the LEDs, lenses and filters are opposite to each other.

[0016] In an optional embodiment, two lens mounts are provided between the excitation filter turntable and the receiving filter turntable. The light source assembly includes a light source mounting base, and the optical signal acquisition assembly includes an acquisition assembly mounting base. Lens slots are respectively provided on the lens mounts, the light source mounting base, and the acquisition assembly mounting base. The lens is pressed and fixed in the lens slot by a lens clamping block.

[0017] In an optional embodiment, a limiting step is provided on the central shaft, and the filter turntable includes a through hole located at the center. The central shaft passes through the through hole and is locked to the limiting step by a nut. One end of the central shaft is connected to the drive motor, and the other end passes through the receiving filter turntable and extends into the interior of the acquisition component mounting base. The acquisition component mounting base and the end of the central shaft are provided with end bearings, and the acquisition component mounting base is provided with bearing grooves for installing the end bearings.

[0018] In an optional embodiment, the light source mounting base includes an LED lamp aluminum substrate base, and the light source assembly further includes a light source mounting bracket fixedly installed inside the LED lamp aluminum substrate base. Rotary bearings are respectively provided between the central shaft and the lens mount, and between the central shaft and the LED lamp aluminum substrate base. Bearing grooves for mounting the rotary bearings are respectively provided on the lens mount and the LED lamp aluminum substrate base.

[0019] In an optional embodiment, a motor adapter is provided between the drive motor and the LED lamp aluminum substrate base. A calibration block is fixedly installed inside the motor adapter. The calibration block has a notch, and a photoelectric sensor for calibration is installed at the notch. A photoelectric baffle that can rotate synchronously and is used for calibration is clamped on the central shaft. The position of the photoelectric baffle blocking the photoelectric sensor corresponds to the initial position of the filter turntable.

[0020] In an optional embodiment, the light source mounting bracket includes an LED lamp aluminum substrate and a light source plate arranged axially back and forth along the central axis, with the central axis gap passing through the light source mounting bracket.

[0021] In an optional embodiment, the light source mounting bracket includes an LED lamp aluminum substrate, which is connected to the central shaft via an adapter clamp. A slip ring is mounted on the central shaft and installed inside a slip ring seat.

[0022] The fluorescence detection module in this application mainly constitutes a standardized rapid detection structure in the fluorescence detection process. The number of modules can be stacked and combined according to market demand to form a variety of complete machines for use, increasing detection speed and efficiency. It can meet diverse needs with limited specifications, thereby shortening the development cycle, reducing costs, improving quality and reliability, and simplifying maintenance and product support.

[0023] Other features and advantages of this application will be described in detail in the following detailed description section. Attached Figure Description

[0024] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 This is a schematic diagram of the fluorescence detection module of the fixed light source assembly in this application; Figure 2 for Figure 1 Internal structure diagram; Figure 3 This is a schematic diagram of the fluorescence detection module of the rotating light source assembly in this application; Figure 4 for Figure 3 Internal structure diagram; Figure 5 This is a schematic diagram of the filter turntable. Figure 6 This is a schematic diagram illustrating the detection principle. Figure 7 This is a schematic diagram of the scanning status of the four channels.

[0026] icon: 1-Light source acquisition board cover; 2-ADC board; 3-Light source acquisition board; 4-Light source acquisition board base; 5a-Rotating bearing; 5b-End bearing; 6-Lens clamping block; 7-Lens; 8-Filter turntable; 8a-Excitation filter turntable; 8b-Receiver filter turntable; 9-Filter clamping cover; 10-Fiber optic cable; 11-Lens connector; 12-Central shaft; 13-LED lamp aluminum substrate base; 14-LED lamp aluminum substrate; 15-Light source board; 16-Photoelectric baffle; 17-Photoelectric sensor; 18-Motor adapter; 19-Drive motor; 20-Filter; 21-Adapter; 22-Copper stud; 23-Slip ring; 24-Slip ring seat; 25-Sample cell; 26-Calibration block; 27-Notch. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0028] In the description of this application, it should be noted that the terms "inner" and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of describing this application and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0029] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "setup" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0030] The fluorescence detection module in this application is suitable for high-throughput fluorescence detection with multiple channels and multiple wells. Through the cooperation of a rotating mechanism, a filter turntable 8, a light source assembly, an optical signal acquisition assembly, and an optical fiber 10, the fluorescence detection module achieves rapid, multi-channel, and high-sensitivity fluorescence signal acquisition from the sample cell 25.

[0031] See Figure 1 and combined Figures 2-7The fluorescence detection module of the present invention includes: a rotating mechanism, a filter turntable 8, a light source assembly, an optical signal acquisition assembly, and an optical fiber 10.

[0032] The light source assembly is used to emit excitation fluorescence into the sample cell 25. The rotating mechanism includes a central shaft 12 and a drive motor 19. The filter turntable 8 is rotatably connected to the central shaft 12, and multiple filters 20 with different filtering bands are provided on the filter turntable 8.

[0033] The filter 20 can be used to filter the excitation fluorescence or the fluorescence received after passing through the sample cell 25, or it can be used to filter both the excitation fluorescence and the fluorescence received after passing through the sample cell 25. The filter can be specifically set by setting different filter turntables 8 before and after passing through the sample cell 25.

[0034] The filter 20 avoids stray light interference and improves the signal-to-noise ratio. At the same time, combined with the synchronous rotation of the filter turntable 8 following the central axis 12, it enables rapid, multi-channel, and high-sensitivity fluorescence signal acquisition from the sample cell 25.

[0035] Fiber 10 connects the light source assembly, sample cell 25 and optical signal acquisition assembly to conduct fluorescence signals.

[0036] The drive motor 19 drives the central shaft 12 to rotate, which in turn drives the filter turntable 8 to rotate synchronously. This is used to ensure that the excitation fluorescence emitted by the light source assembly and / or the received fluorescence after passing through the sample cell 25 are received by the optical signal acquisition assembly after passing through the filter 20 with different filter wavelengths.

[0037] The optical signal acquisition component specifically includes a light source acquisition board cover 1, an ADC board 2, and a light source acquisition board 3. The light source acquisition board 3 is installed in the light source acquisition board base 4, and the light source acquisition board cover 1 and the light source acquisition board base 4 are connected and aligned.

[0038] The scanning time for a single aperture four-channel in the prior art is about 1.6s. This application can realize 8 apertures in a single module by means of a rotating mechanism, and use up to 8 optical paths for rapid scanning. The time can be controlled within 2s. Compared with the prior art, the scanning time and acquisition efficiency have achieved a qualitative leap.

[0039] The fluorescence detection module in this application has an 8-channel optical path for its light source component, and a single-module 8-hole filter disk 8. During operation, after the drive motor 19 is powered on, the central shaft 12 rotates, simultaneously driving the filter disks 8 on both sides to rotate synchronously. The light source component and the optical signal acquisition component work together to intermittently emit and receive optical signals. The fluorescence signal emitted by the light source component is filtered by the filter 20 and then illuminates the sample cell 25 through the lens 7. Simultaneously, the fluorescence signal after passing through the sample cell 25 is filtered by the filter 20 and then received by the optical signal acquisition component through the lens 7. Each hole in the 8-hole structure can use up to 8 channels of light source for optical detection, and all detections can be completed in one rotation. See [link to relevant documentation]. Figure 7 The diagram illustrates the detection process using a 4-channel scan.

[0040] The filter turntable 8 includes at least two, which are mounted at intervals on the central shaft 12, and are capable of filtering the excitation fluorescence and the reception fluorescence simultaneously.

[0041] The two filter turntables 8 include an excitation filter turntable 8a and a receiving filter turntable 8b, respectively. An optical fiber 10 is connected between the excitation filter turntable 8a, the sample cell 25 and the receiving filter turntable 8b, so that the laser fluorescence and the received fluorescence can both pass through the filters 20 of different filtering bands, realizing independent filtering of the excitation optical path and the receiving optical path.

[0042] Overlapping filter covers 9 are provided on both sides of the excitation filter turntable 8a and both sides of the receiving filter turntable 8b, with filters 20 connected between the filter covers 9 and the filter turntables 8. The filters 20 on each filter turntable 8 are axially aligned along the central axis 12, and the filters 20 on the excitation filter turntable 8a and the receiving filter turntable 8b are also axially aligned along the central axis 12. This structure ensures the consistency between the excitation and receiving optical paths, facilitating mass assembly and maintenance.

[0043] To further improve light efficiency and signal strength, lenses 7 are fixedly installed on both sides of the filter turntable 8. The light source assembly includes multiple LEDs. The number of lenses 7, LEDs and filters 20 are corresponding to each other, and in the initial state, the positions of LEDs, lenses 7 and filters 20 are opposite to each other.

[0044] Specifically, from the perspective of lens 7 installation, two lens mounts 11 are provided between the excitation filter turntable 8a and the receiving filter turntable 8b, the light source assembly includes a light source mounting base, and the optical signal acquisition assembly includes an acquisition assembly mounting base.

[0045] Lens slots are provided on the lens mount 11, the light source mount, and the acquisition component mount, and the lens 7 is pressed and fixed in the lens slot by the lens clamping block 6. This arrangement ensures that each optical signal is focused by an independent lens 7 and filtered by a filter 20, which greatly improves the overall efficiency and consistency of the fluorescence detection module.

[0046] To improve rotational stability and assembly accuracy, in this embodiment, a limiting step is provided on the central shaft 12, and the filter turntable 8 includes a through hole located in the center. The central shaft 12 passes through the through hole on the filter turntable 8 and is locked to the limiting step by a nut.

[0047] One end of the central shaft 12 is connected to the drive motor 19, and the other end extends through the receiving filter turntable 8b and into the interior of the acquisition component mounting base. An end bearing 5b is provided at the end of the acquisition component mounting base and the central shaft 12, and a bearing groove for mounting the end bearing 5b is provided on the acquisition component mounting base.

[0048] This configuration allows the central shaft 12 to drive the two sets of filter turntables 8 to rotate synchronously.

[0049] The light source mounting base includes an LED lamp aluminum substrate base 13. The light source assembly also includes a light source mounting bracket fixedly installed inside the LED lamp aluminum substrate base 13. Rotary bearings 5a are respectively provided between the central shaft 12 and the lens connector 11, and between the central shaft 12 and the LED lamp aluminum substrate base 13. Bearing grooves for mounting the rotary bearings 5a are respectively provided on the lens connector 11 and the LED lamp aluminum substrate base 13.

[0050] The above bearing layout effectively reduces rotational friction and ensures the positioning accuracy and repeatability of the filter turntable 8 under high-speed rotation.

[0051] To achieve accurate switching of optical paths in each channel, a motor adapter 18 is provided between the drive motor 19 and the LED lamp aluminum substrate base 13. A calibration block 26 is fixedly installed inside the motor adapter 18. A notch 27 is provided on the calibration block 26, and a photoelectric sensor 17 for calibration is installed at the notch 27.

[0052] A photoelectric baffle 16, which rotates synchronously with the central shaft 12 and is used for calibration, is clamped on the central shaft 12. The position of the photoelectric baffle 16 blocking the photoelectric sensor 17 corresponds to the initial position of the filter turntable 8. The calibration of the initial position can automatically identify the initial position of the filter turntable 8 each time it is powered on or reset, ensuring the accuracy of channel switching.

[0053] The light source assembly is specifically designed as an 8-channel optical path. The multiple LEDs in the light source assembly of this application include 8 LEDs. In addition, in order to meet the testing requirements of different scenarios, the light source assembly includes two different structural forms. In one specific setting, the 8 LEDs are fixedly installed. The light source mounting bracket includes an LED aluminum substrate 14 and a light source plate 15 arranged axially along the central axis 12. The central axis 12 is gapped through the light source mounting bracket, which is suitable for conventional power scenarios.

[0054] In this configuration, during the connection process, two rotating bearings 5a are placed into the bearing grooves on both sides of the lens mount 11 and fixed. Sixteen lenses 7 are then placed into the lens grooves on both sides of the lens mount 11, and then pressed and fixed with sixteen lens clamping blocks 6.

[0055] Place the required wavelength and quantity of filters 20 into the slots on both sides of the filter turntable 8, and then press and fix them with the filter cover 9. Insert the central shaft 12 into the bearing hole in the lens mount 11, and then fix the two assembled excitation filter turntables 8a and receiving filter turntables 8b to the limiting steps on both sides of the central shaft 12, and lock them with nuts.

[0056] The rotating bearing 5a is fixed in the bearing groove on the left side of the LED lamp aluminum substrate holder 13. Eight lenses 7 are then placed in the lens grooves on the left side of the LED lamp aluminum substrate holder 13, and then pressed and fixed with eight lens clamping blocks 6. The end bearing 5b is fixed in the bearing groove on the right side of the light source acquisition board holder 4. Eight lenses 7 are then placed in the lens grooves on the right side of the light source acquisition board holder 4, and then pressed and fixed with eight lens clamping blocks 6.

[0057] Fix the LED lamp aluminum substrate holder 13 to the right side of the lens connector 11, and fix the light source acquisition board holder 4 to the left side of the lens connector 11. Assemble the LED lamp aluminum substrate 14 and the light source board 15, and then fix them to the right side of the LED lamp aluminum substrate holder 13.

[0058] Assemble and install the ADC board 2 and the light source acquisition board 3, then fix them to the left side of the light source acquisition board base 4. Fix the photoelectric baffle 16 to the corresponding position on the right side of the central shaft 12. Fix the photoelectric sensor 17 to the corresponding position on the motor adapter 18.

[0059] Fix the motor adapter 18 to the right side of the LED lamp aluminum substrate base 13. Fix the light source acquisition board cover 1 to the left side of the light source acquisition board base 4. Fix the drive motor 19 to the right side of the motor adapter 18, and then use the set screw to tighten the central shaft 12 inserted into the motor. Insert sixteen optical fibers 10 into the holes on both sides of the middle of the lens connector 11, with each corresponding left and right hole forming a group, for a total of eight groups, and then pass them out through the wiring holes.

[0060] In another specific configuration, the eight LEDs are mounted in a way that allows them to rotate synchronously with the central shaft 12 and the filter turntable 8. The eight LEDs use LED beads of different wavelengths, and a slip ring 23 structure is added to achieve synchronous rotation between the LED aluminum substrate 14 and the central shaft 12.

[0061] Specifically, the light source mounting bracket includes an LED lamp aluminum substrate 14, which is connected to the central shaft 12 via an adapter 21. A slip ring 23 is mounted on the central shaft 12 and is installed inside a slip ring seat 24.

[0062] This setup allows the light source components to rotate synchronously with the central axis 12, making it suitable for test scenarios that require dynamic alignment of the light source or special optical path layout.

[0063] Similarly, the LED lamp aluminum substrate 14 and the light source acquisition board 3 maintain intermittent transmission and reception coordination. One rotation can complete multi-channel detection of all holes of different LED lamps, further improving the system integration and optical path flexibility.

[0064] In this configuration, the left half of the attached diagram and the initial position marking structure on the right side remain unchanged during the connection process. The LED light aluminum substrate 14 is fixed to the adapter 21, the copper stud 22 is fixed to the slip ring 23, and the LED light aluminum substrate 14 and the slip ring 23 are wired and soldered.

[0065] Insert the LED light aluminum substrate 14 and slip ring 23 into the corresponding positions of the central shaft 12, and use set screws to lock the adapter 21. Fix the slip ring seat 24 to the left side structure, and then lock the copper stud 22 onto the slip ring seat 24. Fix the motor adapter 18 to the right side of the slip ring seat 24. Fix the drive motor 19 to the right side of the slip ring seat 24, and then use set screws to tighten the central shaft 12 inserted into the drive motor 19.

[0066] After the drive motor 19 is powered on, the central shaft 12 rotates, which in turn drives the excitation filter turntable 8a and the receiving filter turntable 8b to rotate synchronously with the LED lamp aluminum substrate 14. The LED lamp aluminum substrate 14 and the light source acquisition board 3 work together to intermittently emit and receive light signals. Each of the 8 holes can use up to 8 channels of light source for optical detection. All detections can be completed in one rotation.

[0067] It is worth noting that the fluorescence detection module in this application features an optimized rotary motion compared to conventional PCR instruments, photometers, and microplate readers, supporting a higher number of channels and faster movement. Utilizing fiber optic cable for conduction, the detection speed and efficiency are significantly improved. The modular design results in high product quality, high precision, stable performance, wide versatility, and a compact size with minimal space requirements.

[0068] For multi-channel and multi-well detection, the product offers fast detection time and high data acquisition efficiency. Furthermore, its design adheres to principles of versatility, standardization, and modularity, reducing time, lowering investment, and improving quality and reliability, thus ensuring its broad applicability.

[0069] It should be noted that, where there is no conflict, the features in the embodiments of this application can be combined with each other.

[0070] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A fluorescence detection module, characterized in that, Includes a rotating mechanism, a filter turntable, a light source assembly, an optical signal acquisition assembly, and optical fibers; The light source assembly is used to emit excitation fluorescence into the sample cell; The rotating mechanism includes a central shaft and a drive motor. The filter turntable is rotatably connected to the central shaft, and the filter turntable is provided with multiple filters of different filtering bands. The optical fiber connects the light source assembly, the sample cell, and the optical signal acquisition assembly to conduct fluorescence signals. The drive motor drives the central shaft to rotate, which in turn drives the filter turntable to rotate synchronously. This is used to ensure that the excitation fluorescence emitted by the light source assembly and / or the received fluorescence after passing through the sample cell are received by the optical signal acquisition assembly after passing through filters of different wavelengths.

2. The fluorescence detection module according to claim 1, characterized in that, The filter turntable includes at least two, and the two filter turntables are mounted at intervals on the central shaft; The two filter turntables each include an excitation filter turntable and a receiving filter turntable. The optical fiber is connected between the excitation filter turntable, the sample cell, and the receiving filter turntable, so that the laser fluorescence and the received fluorescence can both pass through filters of different filtering bands.

3. The fluorescence detection module according to claim 2, characterized in that, The excitation filter turntable and the receiving filter turntable are respectively provided with overlapping filter covers, and the filters are respectively connected between the filter covers and the filter turntable. The filters on each of the filter turntables are axially aligned along the central axis, and the filters on the excitation filter turntable and the receiving filter turntable are axially aligned along the central axis.

4. The fluorescence detection module according to claim 3, characterized in that, Lenses are fixedly arranged on both sides of the filter turntable. The light source assembly includes multiple LEDs. The number of lenses, LEDs and filters are corresponding to each other, and in the initial state, the positions of the LEDs, lenses and filters are opposite to each other.

5. The fluorescence detection module according to claim 4, characterized in that, Two lens mounts are provided between the excitation filter turntable and the receiving filter turntable. The light source assembly includes a light source mounting base, and the optical signal acquisition assembly includes an acquisition assembly mounting base. Lens slots are respectively provided on the lens mounts, the light source mounting base, and the acquisition assembly mounting base. The lens is pressed and fixed in the lens slot by a lens clamping block.

6. The fluorescence detection module according to claim 5, characterized in that, A limiting step is provided on the central shaft, and the filter turntable includes a through hole located in the center. The central shaft passes through the through hole and is locked to the limiting step by a nut. One end of the central shaft is connected to the drive motor, and the other end passes through the receiving filter turntable and extends into the interior of the acquisition component mounting base. The acquisition component mounting base and the end of the central shaft are provided with end bearings, and the acquisition component mounting base is provided with bearing grooves for installing the end bearings.

7. The fluorescence detection module according to claim 5, characterized in that, The light source mounting base includes an LED lamp aluminum substrate base, and the light source assembly also includes a light source mounting bracket fixedly installed inside the LED lamp aluminum substrate base. Rotary bearings are respectively provided between the central shaft and the lens mount, and between the central shaft and the LED lamp aluminum substrate base. Bearing grooves for installing the rotary bearings are respectively provided on the lens mount and the LED lamp aluminum substrate base.

8. The fluorescence detection module according to claim 7, characterized in that, A motor adapter is provided between the drive motor and the LED lamp aluminum substrate base. A calibration block is fixedly installed inside the motor adapter. The calibration block has a notch, and a photoelectric sensor for calibration is installed at the notch. A photoelectric baffle that can rotate synchronously and is used for calibration is clamped on the central shaft. The position of the photoelectric baffle blocking the photoelectric sensor corresponds to the initial position of the filter turntable.

9. The fluorescence detection module according to claim 7, characterized in that, The light source mounting bracket includes an LED lamp aluminum substrate and a light source plate arranged axially back and forth along the central axis, with the central axis gap passing through the light source mounting bracket.

10. The fluorescence detection module according to claim 7, characterized in that, The light source mounting bracket includes an LED lamp aluminum substrate, which is connected to the central shaft via an adapter bracket. A slip ring is mounted on the central shaft and installed inside a slip ring seat.

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