An automated light-shielding reaction device and method

Abstract

This invention relates to the fields of analytical chemistry and laboratory equipment technology, and discloses an automated light-shielding reaction device and method. The automated light-shielding reaction device includes a light-shielding housing with a cavity, into which a reaction vessel is placed; a liquid addition assembly including a storage tank, an inlet pipe, and a timed and metered peristaltic pump, with one end of the inlet pipe connected to the storage tank and the other end passing through the light-shielding housing and connected to the reaction vessel; the timed and metered peristaltic pump being located in the middle section of the inlet pipe; a stirring assembly placed within the cavity; a time relay with its input end connected to the timed and metered peristaltic pump and its output end connected to an alarm; and a relay switching assembly including a normally open relay and a normally closed relay, both with their coil ends connected to the output end of the time relay, and their contacts connected to the alarm and the stirring assembly, respectively. This invention achieves automated reaction through the linkage of various components, effectively improving reaction efficiency and the accuracy of experimental results.

Description

Technical Field

[0001] This invention relates to the fields of analytical chemistry and laboratory equipment technology, and in particular to an automated light-shielding reaction apparatus and method. Background Technology

[0002] In food testing, many reactions require light-protected conditions, such as peroxide value and vitamin C detection. Current techniques typically involve placing samples in a drawer or dark box, manually timing, adding solutions, and rinsing. However, this method relies on manual operation, making it prone to errors such as forgetting or timing mistakes that could lead to reaction times that are too long or too short, affecting the accuracy of the results. Furthermore, the solution addition and rinsing processes are cumbersome and inefficient, failing to meet the demands of modern laboratories for high automation and precise control.

[0003] Therefore, there is an urgent need to provide a light-shielding reaction device and method that can improve the accuracy of detection results and has a high degree of automation. Summary of the Invention

[0004] In view of this, the present invention provides an automated light-shielding reaction device and a light-shielding reaction method to solve the problems of poor accuracy and low efficiency of light-shielding reaction detection results in existing food testing processes.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: On one hand, the present invention provides an automated light-shielding reaction device, including a light-shielding chamber, a liquid addition assembly, a stirring assembly, a time relay, and a relay switching assembly; wherein, the light-shielding chamber has a cavity, and a reaction vessel is placed inside the cavity; the liquid addition assembly includes a storage tank, a liquid inlet pipe, and a timed and metered peristaltic pump, one end of the liquid inlet pipe is connected to the storage tank, and the other end passes through the light-shielding chamber and connects to the reaction vessel, and the timed and metered peristaltic pump is located in the middle section of the liquid inlet pipe; the stirring assembly is placed inside the cavity and is used to mix the reactants in the reaction vessel; the input terminal of the time relay is connected to the timed and metered peristaltic pump, and the output terminal is connected to an alarm; the relay switching assembly includes a normally open relay and a normally closed relay, the coil terminal of the normally open relay is connected to the output terminal of the time relay, and the contact terminal is connected to the alarm, the coil terminal of the normally closed relay is connected to the output terminal of the time relay, and the contact terminal is connected to the stirring assembly.

[0006] Preferably, the side wall of the light-proof box is provided with a door, and the outer periphery of the door is provided with a sealing strip.

[0007] Preferably, the inner wall of the light-shielding box is provided with an anti-corrosion coating.

[0008] Preferably, at least one liquid storage tank is provided.

[0009] Preferably, the liquid addition assembly further includes a liquid addition connector, which is detachably connected to one end of the liquid inlet pipe located in the reaction vessel, and the liquid addition connector extends into the reaction vessel for adding liquid to the reaction vessel or rinsing the reaction vessel.

[0010] Preferably, the timed and metered peristaltic pump has at least one channel.

[0011] Preferably, the stirring assembly includes a magnetic stirrer and a stir bar, the reaction vessel is placed on top of the magnetic stirrer, the stir bar is placed inside the reaction vessel, and the stir bar is driven by the drive unit of the magnetic stirrer to move.

[0012] Preferably, the interior of the light-shielding box is provided with a limiting member for fixing the reaction vessel inside the light-shielding box.

[0013] On the other hand, the present invention also provides an automated light-avoidance reaction method using the above-mentioned device, comprising the following steps: (1) Place the reaction container containing the sample into the cavity of the light-proof box, set the target delivery value of the timed and quantitative peristaltic pump, and set the protection time threshold of the time relay at the same time. (2) Start the timed and metered peristaltic pump. The liquid enters the reaction vessel from the storage tank through the inlet pipe. The timed and metered peristaltic pump outputs a metering signal to the time relay in real time. (3) The time relay receives the quantitative signal and continuously monitors whether the target delivery value has been reached. When the target delivery value is reached, the timed quantitative peristaltic pump stops running. (4) The time relay starts timing, and the stirring component starts running at the same time. When the protection time threshold is reached, the time relay outputs a time signal to the normally closed relay, normally open relay and alarm. The stirring component stops running, and the alarm issues an alarm signal, and the reaction terminates.

[0014] Preferably, the method further includes the following steps: replacing the storage tank containing the cleaning solution, and cleaning the inlet component by starting a timed and metered peristaltic pump.

[0015] This invention provides an automated light-shielding reaction device and a light-shielding reaction method, which have the following advantages compared with the prior art: The automated light-shielding reaction device of the present invention uses a light-shielding chamber to ensure that the reaction process is carried out under light-shielding conditions, and achieves automated reaction through the linkage of liquid addition component, stirring component, time relay and relay switching component, realizing precise liquid addition and reminder functions. It can not only avoid the error caused by manual operation and improve reaction efficiency, but also prevent reaction timeout and improve the accuracy of experimental results, thus meeting the needs of modern laboratories for high automation and precise control. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the automated light-avoiding reaction device of the present invention.

[0018] In the diagram: 1-Light-proof enclosure, 2-Liquid storage tank, 3-Liquid inlet pipe, 4-Timed and metered peristaltic pump, 5-Liquid addition connector, 6-Magnetic stirrer, 7-Stirring element, 8-Time relay, 9-Normally open relay, 10-Normally closed relay, 11-Alarm, 12-Reaction vessel. Detailed Implementation

[0019] The present invention will be described below through specific embodiments. Those skilled in the art will understand that the specific embodiments below are merely illustrative and do not limit the scope of the invention in any way. Furthermore, in the following embodiments, unless otherwise specified, the reagents and equipment used are commercially available. If specific processing conditions and methods are not explicitly described in the later embodiments, conditions and methods known in the art can be used for processing.

[0020] In one aspect of the invention, such as Figure 1 As shown, this invention provides an automated light-shielding reaction device, including a light-shielding chamber 1, a liquid addition assembly, a stirring assembly, a time relay 8, and a relay switching assembly. By integrating the light-shielding chamber 1, the liquid addition assembly, the stirring assembly, the time relay 8, and the relay switching assembly, this invention achieves automated sample addition, light-shielding reaction, and alerts, reducing manual intervention and improving experimental efficiency and accuracy.

[0021] In some embodiments of the present invention, the light-shielding chamber 1 has a cavity, and the reaction vessel 12 is placed inside the cavity to provide light-shielding conditions for the reaction, avoid the influence of light on the reaction, and improve the accuracy of the experiment. The reaction vessel 12 can be selected according to the actual situation and is not particularly limited thereto; for example, it can be a 250mL conical flask.

[0022] In some embodiments of the present invention, in order to facilitate the placement and removal of the reaction vessel 12, the side wall of the light-proof box 1 is provided with a door, and the outer periphery of the door is provided with a sealing strip to ensure that the light-proof box 1 is completely light-proof, creating an ideal light-proof environment for subsequent reactions.

[0023] In some embodiments of the present invention, the inner wall of the light-shielding box 1 is provided with an anti-corrosion coating. Since liquid may splash out during the liquid addition process, the anti-corrosion coating can improve the corrosion resistance of the light-shielding box 1 and further extend its service life. The choice of anti-corrosion coating is not particularly limited; conventional coatings are sufficient.

[0024] In some embodiments of the present invention, the side wall of the light-shielding box 1 is provided with a through hole, and the liquid addition pipe passes through the through hole to connect to the reaction vessel 12. In order to ensure the light-shielding performance of the light-shielding box 1, there is no gap between the outer periphery of the liquid addition pipe and the through hole. If there is a gap, it needs to be sealed. The sealing method can be a sealing strip or a sealant.

[0025] In some embodiments of the present invention, a limiting member is provided inside the light-shielding box 1 for fixing the reaction container 12 inside the light-shielding box 1, so that the reaction container 12 can always be on the magnetic stirrer 6 and avoid significant displacement. The structure of the limiting member is not particularly limited, as long as it can fix the reaction container 12 to a certain extent, such as by means of clamps.

[0026] In some embodiments of the present invention, the liquid addition assembly includes a storage tank 2, an inlet pipe 3, and a timed and metered peristaltic pump 4. One end of the inlet pipe 3 is connected to the storage tank 2, and the other end passes through a light-proof enclosure 1 and connects to the reaction vessel 12. The timed and metered peristaltic pump 4 is located in the middle section of the inlet pipe 3. Specifically, the liquid in the storage tank 2 is transported to the reaction vessel 12 via the inlet pipe 3 and the timed and metered peristaltic pump 4 for subsequent reaction. The flow rate of the timed and metered peristaltic pump 4 can be adjusted within the range of 0.1-10 mL / min.

[0027] In some embodiments of the present invention, the liquid addition assembly further includes a liquid addition connector 5, which is detachably connected to one end of the liquid inlet pipe 3 located in the reaction vessel 12, and extends into the reaction vessel 12 for adding liquid to or rinsing the reaction vessel 12. The liquid addition connector 5 can be replaced according to the purpose of the liquid addition assembly. For example, when the liquid addition assembly is used to add reaction liquid to the reaction vessel 12, the liquid addition connector 5 can be a dropper nozzle to avoid splashing of the reaction liquid; while when the liquid addition assembly is used to add cleaning liquid to the reaction vessel 12, the liquid addition connector 5 can be a spray nozzle to achieve thorough cleaning of the reaction vessel 12.

[0028] In some embodiments of the present invention, at least one liquid storage tank 2 is provided, and the number of channels of the timed and metered peristaltic pump 4 is at least one. The specific number of liquid storage tanks 2 can be increased or decreased according to the liquid addition situation, or timed and metered peristaltic pumps 4 with different numbers of channels can be selected according to the liquid addition situation. For example, when it is necessary to add two kinds of liquids to the reaction vessel 12, two liquid storage tanks 2 and a dual-channel timed and metered peristaltic pump 4 are used, and the parameters of the fluid in each channel of the timed and metered peristaltic pump 4 can be set individually to achieve independent control of different liquids.

[0029] In some embodiments of the present invention, the stirring assembly is placed inside a cavity for mixing the reactants in the reaction vessel 12. The stirring assembly includes a magnetic stirrer 6 and a stir bar 7. The reaction vessel 12 is placed on top of the magnetic stirrer 6, and the stir bar 7 is placed inside the reaction vessel 12. The stir bar 7 is driven by the drive unit of the magnetic stirrer 6 to move, achieving uniform mixing and reaction of the sample through non-contact transmission. The rotation speed range can be set to 50-1500 rpm.

[0030] In some embodiments of the present invention, the input terminal of the time relay 8 is connected to the timed and metered peristaltic pump 4, and the output terminal is connected to an alarm 11. The time relay 8 is used to receive the metering signal output by the timed and metered peristaltic pump 4 in real time to determine whether the liquid addition process has ended and to proceed to the next process.

[0031] In some embodiments of the present invention, the relay switching assembly includes a normally open relay 9 and a normally closed relay 10. The coil terminal of the normally open relay 9 is connected to the output terminal of the time relay 8, and its contact terminal is connected to the alarm 11. The coil terminal of the normally closed relay 10 is connected to the output terminal of the time relay 8, and its contact terminal is connected to the stirring assembly. Through the coordinated operation of the time relay 8, the normally open relay 9, the normally closed relay 10, the stirring assembly, and the alarm 11, the power can be automatically cut off and a reminder can be given after the reaction is completed, preventing the reaction from exceeding the time limit and ensuring the accuracy of the experimental results.

[0032] In another aspect, the present invention also provides an automated light-avoidance reaction method using the above-described device, comprising the following steps: (1) Place the reaction container 12 containing the sample into the cavity of the light-proof box 1, set the target delivery value of the timed and quantitative peristaltic pump 4, and set the protection time threshold of the time relay 8 at the same time; the protection time threshold is the light-proof reaction time after the liquid addition is completed, which can be set to different durations such as 3min, 7min, 60min according to the specific reaction requirements. (2) Start the timed and metered peristaltic pump 4. The liquid enters the reaction vessel 12 from the storage tank 2 through the inlet pipe 3. The timed and metered peristaltic pump 4 outputs a metering signal to the time relay 8 in real time. (3) The time relay 8 receives the quantitative signal and continuously monitors whether the target delivery value is reached. When the target delivery value is reached, the timed quantitative peristaltic pump 4 stops running. (4) Time relay 8 starts timing, and at the same time the stirring component starts running. When the protection time threshold is reached, time relay 8 outputs a time signal to normally closed relay 10, normally open relay 9 and alarm 11. The stirring component stops running, and at the same time the alarm 11 issues an alarm signal, and the reaction terminates.

[0033] It should be noted that before the time relay 8 outputs the time signal, the normally closed relay 10 remains closed and the normally open relay 9 remains open. After the time relay 8 outputs the time signal, it triggers the normally open relay 9 to close to control the alarm 11 to issue an alarm, and at the same time triggers the normally closed relay 10 to open so that the stirring component stops running and the reaction terminates.

[0034] In some embodiments of the present invention, the following steps are also included: replacing the storage tank 2 containing the cleaning fluid, and cleaning the inlet component by starting the timed and metered peristaltic pump 4.

[0035] In another aspect, the present invention provides an application of the above-mentioned automated light-shielding reaction device and reaction method in food safety testing, including but not limited to the dark reaction stage required for peroxide value determination in GB 5009.227-2023, or the light-shielding sample treatment required for ascorbic acid determination in GB 5009.86-2025.

[0036] In some embodiments of the present invention, the process of using the device and method to detect the peroxide value in oils is as follows: (1) Place the conical flask containing 2-3g of oil sample into the cavity of the light-proof box 1, set the target delivery value of the timed and quantitative peristaltic pump 4 (30mL of chloroform-glacial acetic acid solution and 1.00mL of potassium iodide saturated solution), and set the protection time threshold of the time relay 8 to 3min; (2) Start the channel timed and quantitative peristaltic pump 4, and the liquid enters the reaction container 12 from the storage tank 2 through the liquid inlet pipe 3. The timed and quantitative peristaltic pump 4 outputs a quantitative signal to the time relay 8 in real time; (3) The time relay 8 receives the quantitative signal and continuously monitors whether the target delivery value is reached. When the target delivery value is reached, the timed and quantitative peristaltic pump 4 stops running; (4) The time relay 8 starts timing, and the stirring component starts running at the same time. When the protection time threshold is reached, the time relay 8 outputs a timeout signal to the normally closed relay 10, the normally open relay 9 and the alarm 11. The stirring component stops running, and the alarm 11 issues an alarm signal, and the reaction terminates. This process ensures that the reaction is carried out under completely dark conditions and effectively prevents the reaction time from affecting the iodine precipitation result.

[0037] In some embodiments of the present invention, the process of using the device and method to detect ascorbic acid in fruits or vegetables is as follows: (1) Place a conical flask containing 20g of fruit or vegetable sample and 2g of acidic activated carbon into the cavity of the light-proof box 1, set the target delivery value of the timed and quantitative peristaltic pump 4, and set the protection time threshold of the time relay 8 to 60min; (2) Start the timed and quantitative peristaltic pump 4, and the reaction reagent enters the reaction container 12 from the storage tank 2 through the inlet pipe 3. The timed and quantitative peristaltic pump 4 outputs a quantitative signal to the time relay 8 in real time; (3) The time relay 8 receives the quantitative signal and continuously monitors whether the target delivery value is reached. When the target delivery value is reached, the timed and quantitative peristaltic pump 4 stops running; (4) The time relay 8 starts timing, and the stirring component starts running at the same time. When the protection time threshold is reached, the time relay 8 outputs a timeout signal to the normally closed relay 10, the normally open relay 9 and the alarm 11. The stirring component stops running, and the alarm 11 issues an alarm signal, and the reaction terminates. This process ensures that the reaction is not affected by light, and the automated liquid addition reduces human contact, making it particularly suitable for high-sensitivity detection with a detection limit of 3.00 mg / 100 g.

[0038] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. An automated light-protected reaction device, characterized in that, The application relates to a light-proof box body, a liquid adding assembly, a stirring assembly and a time relay. The light-proof box body has a cavity, and a reaction container is arranged in the cavity; the liquid adding assembly comprises a liquid storage tank, a liquid inlet pipeline and a time-quantity peristaltic pump, one end of the liquid inlet pipeline is connected with the liquid storage tank, the other end of the liquid inlet pipeline penetrates through the light-proof box body and is connected with the reaction container, and the time-quantity peristaltic pump is arranged in the middle section of the liquid inlet pipeline; the stirring assembly is arranged in the cavity and is used for mixing reactants in the reaction container; the time relay is connected with the time-quantity peristaltic pump, and an alarm is connected with the output end of the time relay; the relay switching assembly comprises a normally open relay and a normally closed relay, the coil end of the normally open relay is connected with the output end of the time relay, the contact end of the normally open relay is connected with the alarm, the coil end of the normally closed relay is connected with the output end of the time relay, and the contact end of the normally closed relay is connected with the stirring assembly. The side wall of the light-proof box body is provided with a box door, and a sealing strip is arranged on the outer periphery of the box door. The inner wall of the light-proof box body is provided with an anticorrosive coating. The liquid storage tank is provided with at least one. The liquid adding assembly further comprises a liquid adding connector which is detachably connected with the liquid inlet pipeline at the end of the liquid inlet pipeline located in the reaction container, and the liquid adding connector extends into the reaction container and is used for adding liquid into the reaction container or flushing the reaction container.

2. The automated light protected reaction apparatus of claim 1, wherein, The time-quantity peristaltic pump has at least one channel. The stirring assembly comprises a magnetic stirrer and a stirring rod, the reaction container is arranged on the top of the magnetic stirrer, the stirring rod is arranged in the reaction container, and the stirring rod is driven by the driving unit of the magnetic stirrer to move.

3. The automated light protected reaction apparatus of claim 1, wherein, The inside of the light-proof box body is provided with a limiting piece for fixing the reaction container in the light-proof box body.

4. The automated light protected reaction apparatus of claim 1, wherein, The application further comprises the following steps:

5. The automated light protected reaction apparatus of claim 1, wherein, (1) placing a reaction container containing a sample into the cavity of the light-proof box body, setting a target delivery value of the time-quantity peristaltic pump, and setting a protection time threshold value of the time relay; 6. The automated light protected reaction apparatus of claim 1, wherein, (2) starting the time-quantity peristaltic pump, and making liquid in the liquid storage tank enter the reaction container through the liquid inlet pipeline, and the time-quantity peristaltic pump outputs a quantitative signal to the time relay in real time; 7. The automated light protected reaction apparatus of any one of claims 1-6, wherein, (3) the time relay receives the quantitative signal and continuously monitors whether the target delivery value is reached, and when the target delivery value is reached, the time-quantity peristaltic pump stops running; 8. An automated photoreaction method using the apparatus according to any one of claims 1 to 7, characterized in that, (4) the time relay starts timing, and the stirring assembly starts running at the same time, when the protection time threshold value is reached, the time relay outputs a time signal to the normally closed relay, the normally open relay and the alarm, the stirring assembly stops running, the alarm sends an alarm signal, and the reaction is terminated. The application further comprises the following steps: replacing the liquid storage tank containing cleaning liquid, and cleaning the liquid adding assembly by starting the time-quantity peristaltic pump. ​ ​ 9. The automated light-protected reaction method of claim 8, wherein, ​ ​

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