A near-infrared fluorescent probe specifically targeting a beta amyloid 42 and a preparation method and application thereof

By designing a near-infrared fluorescent probe specifically targeting A42, and utilizing conjugated structure and intramolecular charge transfer, highly selective fluorescence imaging of A42 aggregates is achieved, solving the problem of difficulty in distinguishing A40 and A42 in existing technologies, and providing an effective tool for AD pathology research.

CN122079978BActive Publication Date: 2026-07-07THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
Filing Date
2026-04-27
Publication Date
2026-07-07

AI Technical Summary

Technical Problem

Existing technologies struggle to accurately distinguish between A40 and A42, limiting our understanding of the mechanism of A plaque action in the pathological process of Alzheimer's disease. Furthermore, small molecule probes with this ability to differentiate between them are scarce.

Method used

A near-infrared fluorescent probe specifically targeting A42 was designed. Through its extended conjugated structure and strong intramolecular charge transfer, near-infrared fluorescence emission was achieved. Combined with in vitro spectroscopy experiments and tissue section staining, it provides high selectivity and co-localization effect for A42 aggregates.

Benefits of technology

It achieves high selectivity and near-infrared luminescence capability for A42 aggregates, strong microscopic imaging capability, and efficient staining in both in vivo and ex vivo tissue sections, providing a tool for the diagnosis of AD disease. Moreover, the preparation method is simple, low-cost, and easy to control.

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Abstract

The application belongs to the technical field of biochemistry, and relates to a near-infrared fluorescent probe for specifically targeting A42 and a preparation method and application thereof. The preparation method of the near-infrared fluorescent probe for specifically targeting A42 comprises the following steps: adding a compound (I), a compound (II) and piperidine into a solvent, and performing a reaction to obtain the near-infrared fluorescent probe. The preparation method of the fluorescent probe provided in the application adopts raw materials which are easy to obtain, and the target product can be obtained through only one step of reaction. The reaction condition is mild and easy to control, reaction cost is saved, and the yield of the target product is ensured. The fluorescent probe provided in the application has high micro-imaging capacity, can be used for staining and imaging of A42 aggregates in an ex vivo tissue slice, provides a reliable means for detection of A42, and provides a potential tool for diagnosis of AD disease.
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