Pesticidal composition containing silicon-rich ring and its application in prevention and treatment of cucumber bacterial angular spot disease
By preparing a pesticide composition containing silicon-rich rings and combining it with grape seed and jasmine extract, the problem of poor efficacy in controlling bacterial angular leaf spot of cucumber in existing technologies was solved, achieving a highly efficient disease control effect with a control rate of 80.29%.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-16
- Publication Date
- 2026-05-29
AI Technical Summary
In existing technologies, the effect of quinoline copper in controlling bacterial angular leaf spot of cucumber is limited, and for a long time there has been no effective method to use quinoline copper, silicone ring, grape seed and fennel together to control this disease.
A pesticide composition containing silicon-rich rings, including silicone, copper quinoline, and a synergistic extract composed of grape seed extract and angelica extract, is used for the control of bacterial angular leaf spot of cucumber by preparing suspensions, emulsions, or powders.
In indoor and field trials, the composition significantly inhibited the growth of *Pseudomonas amygdalinus* lesions, and its control effect was superior to that of using silanol or quinoline copper alone, achieving a relative control efficacy of 80.29%, effectively controlling bacterial angular leaf spot in cucumbers.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of biocidal pesticide technology, specifically to a silicon-containing ring-rich pesticide composition and its application in the control of bacterial angular leaf spot in cucumber. Background Technology
[0002] cucumber( Cucumber Cucumber (Cucumis melo) is an annual climbing herbaceous plant belonging to the genus Cucumis in the family Cucurbitaceae. Originally from the southern foothills of the Himalayas, cucumbers are now distributed worldwide. Cucumbers have a unique flavor, crisp texture, high water content, rich nutrients, and are easily digestible, primarily used as a fresh, cooked, pickled, and juiced food. However, with the continuous expansion of cultivation areas, many problems have gradually emerged. Among them, bacterial angular leaf spot of cucumbers is caused by bacteria of the genus Pseudomonas, such as *Pseudomonas amygdalinus*. Pseudomonas almonds pv. Lachrymander This plant disease, caused by [unspecified pathogen], primarily affects cucurbits (such as cucumbers and melons) and solanaceous crops. Typical symptoms include water-soaked, yellowish-brown spots on leaves, which later dry up and perforate. Infected stems or fruits secrete white bacterial ooze, severely impacting cucumber quality and yield.
[0003] Chunlei quinoline copper is a mixture of kasugamycin and quinoline copper. Kasugamycin is an agricultural antibiotic with strong systemic activity, primarily interfering with the amino acid metabolism esterase system, thus affecting protein synthesis, inhibiting hyphal elongation, and causing cell granulation, but it has no effect on spore germination. Quinoline copper is an organic copper chelate with good preventive and curative effects against fungal and bacterial diseases. It can form a tight protective film on the crop surface, inhibiting pathogen germination and invasion, thereby achieving the purpose of disease prevention and treatment. However, because the scope of action of chunlei quinoline copper is limited when used alone, it is often used in combination in actual production. There are various ways to combine it, such as combining it with other synthetic drugs, natural drugs, and their extracts.
[0004] Chloromethylsilatrane is an organosilicon plant growth regulator, belonging to the heteroazosilane tricyclic compound class, with the chemical name 1-chloromethyltriethoxyhexazosilane tricyclic. Chloromethylsilatrane is an organosilicon compound with a unique molecular structure and significant biological activity. Its coordination bonds possess electronic induction capabilities, and their energy can induce seed cell division, enhancing the mitosis of rooting cells and the biosynthesis of proteins. During seed germination, the number of rooting points increases, allowing the plant to fully absorb water and nutrients from the soil during its early development, laying the material foundation for later growth. After the plant absorbs this regulator, its molecules enter the leaves, gradually releasing their electronic induction function. The energy is used to catalyze photosynthesis, enhancing chlorophyll synthesis and continuously forming carbohydrates through the leaves, serving as reserve nutrients for the plant's survival and ultimately supplying the fruit.
[0005] Grape( Wine grapevine Grapes (Vitis vinifera) are tall, twining vines belonging to the genus Vitis in the family Vitaceae. Their berries are oval to oblong-ovate, turning purplish-black or reddish-green when ripe. As a traditional food, grapes are eaten fresh and also used to make dried fruit, preserves, and candied fruit. They are also used as raw materials in the food industry. Although grapes are widely used, grape seeds are generally not used as food and are discarded.
[0006] Fragrant Blue Orchid ( Moldavian dragonfly L.) is a plant belonging to the genus L. in the Lamiaceae family. It is an annual herb distributed in China, Siberia in Russia, Eastern Europe, and Central Europe. It grows in dry mountainous areas, valleys, and rocky riverbanks. It prefers warm, sunny conditions, is drought-tolerant, and highly adaptable. L. can be used as a spice in confectionery and cosmetics. The whole plant can be used medicinally; it is bitter, cool, and has the effects of clearing heat and drying dampness, cooling the liver, and stopping bleeding.
[0007] Although there is some understanding of the efficacy of quinoline copper, silicone ring, grape seed extract, and fennel extract, the efficacy of using these substances together to prevent and control bacterial angular leaf spot in cucumbers has long been unknown. Summary of the Invention
[0008] In view of the above-mentioned prior art, the purpose of this invention is to provide a pesticide composition containing silicon-rich rings and its application in the control of bacterial angular leaf spot of cucumber.
[0009] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides a pesticide composition containing a silicon-rich ring, wherein the pesticide composition uses a silicon-rich ring, copper quinoline, and a synergistic extract as active ingredients; the synergistic extract includes at least one of grape seed extract and jasmine extract.
[0010] Furthermore, the grape seed extract is prepared by the following method: Grape seeds were collected, washed, dried in an oven at 60℃, and pulverized to pass through a 300-mesh sieve to obtain grape seed powder. The grape seed powder was extracted in 75% v / v ethanol at a mass-to-volume ratio of 1g:3ml at room temperature for 12 hours to obtain an extract. The solids after filtering to remove the extract were added to pure water at a mass-to-volume ratio of 1g:10ml and heated. Initial heating was rapid, and the mixture was kept at a gentle boil for 2 hours. After heating was stopped, the mixture was allowed to stand for 40 minutes to remove the solids and aqueous phase, obtaining a secondary extract. The obtained extract and the secondary extract were combined and concentrated under reduced pressure at 60℃ to obtain the grape seed extract.
[0011] Furthermore, the *Cymbidium ensifolium* extract is prepared by the following method: Take the whole flower of *Cymbidium faberi*, remove the calyx, wash and dry in an oven at 70℃, then pulverize to 300 mesh and sieve to obtain *Cymbidium faberi* powder; extract the *Cymbidium faberi* powder in 75% v / v ethanol at room temperature at a mass-to-volume ratio of 1g:2ml, each extraction time is 12h, and a total of 3 extractions are performed; combine the extracts obtained each time, concentrate under reduced pressure at 55℃, and then dry in an oven at 75℃ to obtain *Cymbidium faberi* extract.
[0012] Furthermore, the synergistic extract is a mixture of grape seed extract and jasmine extract, wherein the ratio of grape seed extract to jasmine extract is (1-2)g:(1-2)g.
[0013] Furthermore, the formulation of the pesticide composition includes suspension concentrate, emulsion, microemulsion, or powder.
[0014] Furthermore, the pesticide composition comprises the following components in parts by weight: 20-60 parts of silicone soluble powder, 20-60 parts of quinoline copper, and 2-10 parts of synergistic extract.
[0015] Furthermore, in the silanol soluble powder, the content of silanol is not less than 50 wt%.
[0016] Furthermore, in the kasugamycin and quinoline copper, the sum of the contents of kasugamycin and quinoline copper is not less than 30 wt%.
[0017] A second aspect of the present invention provides the application of the aforementioned silicon-containing cyclic pesticide composition in the control of plant diseases, including bacterial angular leaf spot of cucumber.
[0018] The beneficial effects of this invention are: (1) This invention extracts grape seeds and angelica to obtain grape seed extract and angelica extract. In indoor experiments, the diameter of the colony of Pseudomonas amygdalinus causal disease was reduced in petri dishes with grape seed extract or angelica extract added, indicating that both grape seed extract and angelica extract can inhibit the growth of Pseudomonas amygdalinus causal disease and can be used alone or in combination to prevent and control bacterial angular leaf spot of cucumber.
[0019] (2) This invention uses grape seed extract and *Pseudomonas aeruginosa* extract as synergistic extracts, combined with silicone-containing rings and copper oxychloride, to prepare a pesticide composition containing silicone-containing rings. Field trials showed that, compared to the control group, the disease index of each experimental group was reduced, indicating that the *Pseudomonas aeruginosa* lacrimation-causing pathogen was inhibited. The complex group showed the highest relative efficacy, reaching 80.29%, which was superior to the groups using silicone-containing rings and copper oxychloride, grape seed extract, or *Pseudomonas aeruginosa* alone, as well as the grape seed group and the *Pseudomonas aeruginosa* group. Therefore, the pesticide composition containing silicone-containing rings can better inhibit *Pseudomonas aeruginosa* lacrimation-causing pathogens and is suitable for pesticide development to control bacterial angular leaf spot of cucumber. Detailed Implementation
[0020] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.
[0021] To enable those skilled in the art to better understand the technical solution of this application, the technical solution of this application will be described in detail below with reference to specific embodiments.
[0022] The experimental materials used in the embodiments of this invention, unless otherwise specified, are all conventional experimental materials in the art and can be purchased through commercial channels. The kasugamycin-quinoline copper used in this invention (commercially known as 33% kasugamycin·quinoline copper, commonly available in 100g / bottle) is a suspension concentrate, with active ingredients of 3wt% kasugamycin and 30wt% quinoline copper. The silanol used is a silanol soluble powder, with an active ingredient content of 50wt%, and the CAS number of the silanol is 42003-39-4.
[0023] Example 1: Preparation of Synergistic Extract Preparation of grape seed extract Grape seeds were collected, washed, dried in an oven at 60℃, and pulverized to pass through a 300-mesh sieve to obtain grape seed powder. The grape seed powder was extracted in 75% v / v ethanol at a mass-to-volume ratio of 1g:3ml at room temperature for 12 hours to obtain an extract. The solids after filtering to remove the extract were added to pure water at a mass-to-volume ratio of 1g:10ml and heated. Initial heating was rapid, and the mixture was kept at a gentle boil for 2 hours. After heating was stopped, the mixture was allowed to stand for 40 minutes to remove the solids and aqueous phase, obtaining a secondary extract. The obtained extract and the secondary extract were combined and concentrated under reduced pressure at 60℃ to obtain the grape seed extract.
[0024] Preparation of Orchid Extract Take the whole flower of *Cymbidium faberi*, remove the calyx, wash and dry in an oven at 70℃, then pulverize to 300 mesh and sieve to obtain *Cymbidium faberi* powder; extract the *Cymbidium faberi* powder in 75% v / v ethanol at room temperature at a mass-to-volume ratio of 1g:2ml, each extraction time is 12h, and a total of 3 extractions are performed; combine the extracts obtained each time, concentrate under reduced pressure at 55℃, and then dry in an oven at 75℃ to obtain *Cymbidium faberi* extract.
[0025] Example 2: Indoor Test The bacterial angular leaf spot pathogen used in this invention was collected from Tai'an, Shandong Province. It was taken from leaves infected with bacterial angular leaf spot, and the pathogen was isolated from the diseased tissue and purified. Through Koch's postulates and morphological and molecular biological identification, it was determined to be *Pseudomonas amygdalinus*, the main pathogen causing bacterial angular leaf spot in cucumber. Pseudomonas almonds pv. Lachrymander The obtained pathogenic strain of *Pseudomonas amygdalina* causing lacrimal discharge was inoculated and preserved on LB medium for subsequent experiments.
[0026] Under aseptic conditions, 0.05-0.2 g (1 ml is recorded as 1 g) of grape seed extract or 0.05-0.2 g of *Illicium verum* extract prepared in Example 1 were added to 100 ml of water as test solutions. *Cucumber angular leaf spot* bacteria were evenly spread on LB agar plates with a diameter of 9 cm. Sterile filter paper discs with a diameter of 6 mm were placed at the center of the plate and at four equidistant points 2.5 cm from the center. 20 μL of the above test solution was added to the filter paper disc using a pipette, allowing the disc to be moistened and spread flat on the culture medium. Sterile water was used as a control (CK) at the center point. Each treatment was repeated three times. The petri dishes were placed in an incubator at (27±2)℃. After 48 h, the diameter of the inhibition zone was measured using the cross-hatching method, and the inhibition rate was calculated to compare the antibacterial effects of different treatments.
[0027] Average diameter of inhibition zone = ∑(total diameter of inhibition zone - diameter of filter paper) ÷ 3 Inhibition rate (%) = Average diameter of inhibition zone / Diameter of petri dish × 100 The inhibition rate of bacterial growth is shown in Table 1.
[0028] Table 1. Growth inhibition rate of strains As shown in Table 1, compared with the control group, the colony diameter of *Pseudomonas amygdalinus* causal agent was reduced in the petri dishes with added grape seed extract or *Cymbidium ensifolium* extract. Therefore, both grape seed extract and *Cymbidium ensifolium* extract can inhibit the growth of *Pseudomonas amygdalinus* causal agent and can be used in the development of pesticides for the prevention and control of bacterial angular leaf spot in cucumber.
[0029] Example 2: Preparation of a silicon-containing ring-rich pesticide composition The pesticide composition containing silicon-rich rings includes the following components: 45g of silicon-rich ring soluble powder, 50g of quinoline copper kasugamycin, and 5g of synergistic extract.
[0030] The synergistic extract used was a mixture of grape seed extract and jasmine extract prepared in Example 1, wherein the ratio of grape seed extract to jasmine extract was 1g:1g.
[0031] The preparation method is as follows: Take the proportions of each component according to the formula, add the well-mixed synergistic extract to quinoline copper, mix well, then add the silicon-rich ring soluble powder, mix well, and obtain a pesticide composition containing silicon-rich rings.
[0032] Example 3: Preparation of a silicon-containing ring-rich pesticide composition The pesticide composition containing silicon-rich rings includes the following components: 45g of silicon-rich ring soluble powder, 50g of quinoline copper kasugamycin, and 5g of synergistic extract.
[0033] The synergistic extract used was a mixture of grape seed extract and jasmine extract prepared in Example 1, wherein the ratio of grape seed extract to jasmine extract was 2g:1g.
[0034] The preparation method is as follows: Take the proportions of each component according to the formula, add the well-mixed synergistic extract to quinoline copper, mix well, then add the silicon-rich ring soluble powder, mix well, and obtain a pesticide composition containing silicon-rich rings.
[0035] Example 4: Preparation of a silicon-containing ring-rich pesticide composition The pesticide composition containing silicon-rich rings includes the following components: 45g of silicon-rich ring soluble powder, 50g of quinoline copper kasugamycin, and 5g of synergistic extract.
[0036] The synergistic extract used was a mixture of grape seed extract and jasmine extract prepared in Example 1, wherein the ratio of grape seed extract to jasmine extract was 1g:2g.
[0037] The preparation method is as follows: Take the proportions of each component according to the formula, add the well-mixed synergistic extract to quinoline copper, mix well, then add the silicon-rich ring soluble powder, mix well, and obtain a pesticide composition containing silicon-rich rings.
[0038] Comparative Example 1: Preparation of Pesticide Compositions The difference between Comparative Example 1 and Example 2 is that no synergistic extract was added. Specifically: The pesticide composition containing silicon-rich rings includes the following components: 45g of silicon-rich ring soluble powder and 50g of quinoline copper kasugamycin.
[0039] The preparation method is as follows: Take the proportions of each component according to the formula, add quinoline copper to the silicon-rich ring soluble powder, mix evenly, and obtain a pesticide composition containing silicon-rich rings.
[0040] Comparative Example 2: Preparation of Pesticide Compositions The difference between Comparative Example 2 and Example 2 is that the synergistic extract used is grape seed extract.
[0041] The pesticide composition containing silicon-rich rings includes the following components: 45g of silicon-rich ring soluble powder, 50g of quinoline copper kasugamycin, and 5g of synergistic extract.
[0042] The synergistic extract used was the grape seed extract prepared in Example 1.
[0043] The preparation method is as follows: Take the proportions of each component according to the formula, add the synergistic extract to quinoline copper, mix evenly, then add the silicon-rich ring soluble powder, mix evenly, and obtain a pesticide composition containing silicon-rich rings.
[0044] Comparative Example 3: Preparation of Pesticide Compositions The difference between Comparative Example 3 and Example 2 is that the synergistic extract used is *Cymbidium ensifolium* extract.
[0045] The pesticide composition containing silicon-rich rings includes the following components: 45g of silicon-rich ring soluble powder, 50g of quinoline copper kasugamycin, and 5g of synergistic extract.
[0046] The synergistic extract used was the mixture of orchid extracts prepared in Example 1.
[0047] The preparation method is as follows: Take the proportions of each component according to the formula, add the synergistic extract to quinoline copper, mix evenly, then add the silicon-rich ring soluble powder, mix evenly, and obtain a pesticide composition containing silicon-rich rings.
[0048] Experimental example: Field trial The experiment was conducted in a vegetable garden in Tai'an City, Shandong Province, using the cucumber variety "Qiuguan 55". Each treatment was replicated in four places, with randomly assigned plots of 30 m². 2 The cucumbers were in the early flowering stage when the first application of the pesticide was made, and the second application was made a week later.
[0049] Experimental Methods: Field control trials were conducted using the silicon-containing ring-rich pesticide composition prepared in Example 2 and the pesticide compositions prepared in Comparative Examples 1-3. The groups were set as follows: Complex group: the pesticide used was from Example 2, with the added synergistic extracts being grape seed extract and *Illicium verum* extract prepared in Example 1; Silicon-containing ring-rich group: the pesticide used was from Comparative Example 1, without the added synergistic extract; Grape seed group: the pesticide used was from Comparative Example 2, with the added synergistic extract being grape seed extract prepared in Example 1; *Illicium verum* group: the pesticide used was from Comparative Example 3, with the added synergistic extract being *Illicium verum* extract prepared in Example 1. These four groups were the experimental groups. A control group was also set up, using an equal volume of sterile pure water instead of pesticides. The pesticide solutions used in the field trials were diluted in pure water at a ratio of 1g:1000ml (1ml is considered 1g) for each group, and sprayed evenly using a sprayer. The dosage of pesticide solution per acre was 45kg.
[0050] Investigation Method: Fourteen days after the second application of pesticide, a five-point diagonal sampling method was used in each plot, with three plants at each point, and all leaves were investigated. The pesticides were graded according to the national standard "GB / T17980.110-2004 Field Efficacy Testing Guidelines for Pesticides (II) Part 110: Control of Cucumber Bacterial Angular Leaf Spot by Fungicides" for field efficacy testing of cucumber bacterial angular leaf spot. The grading standards were: Grade 0: No lesions; Grade 1: Lesion area less than 5% of the total leaf area; Grade 3: Lesion area 6%-10% of the total leaf area; Grade 5: Lesion area 11%-20% of the total leaf area; Grade 7: Lesion area 21%-50% of the total leaf area; Grade 9: Lesion area more than 51% of the total leaf area. The severity of disease on the leaves of each plot was investigated, and the disease index and control effect were calculated.
[0051] Disease index = ∑(number of diseased leaves at each level × relative grade value) / (total number of leaves surveyed × 9) × 100 Control efficacy (%) = [1 - (Disease index before treatment in the blank control area × Disease index after treatment in the treated area) / (Disease index after treatment in the blank control area × Disease index before treatment in the treated area)] × 100 Table 2. Field control efficacy of bacterial angular leaf spot in cucumber As shown in Table 2, compared with the control group, the disease index of each experimental group was reduced, indicating that the pathogenic species of *Pseudomonas tonsillar* with lacrimal discharge was inhibited. Among them, the complex group had the highest relative efficacy, reaching 80.29%, which was superior to the silicone ring group, grape seed group, and *Cymbidium ensifolium* group. Therefore, the pesticide composition containing silicone ring, prepared by combining quinoline copper, silicone ring, and synergistic extracts containing grape seed extract and *Cymbidium ensifolium* extract, can better inhibit the pathogenic species of *Pseudomonas tonsillar* with lacrimal discharge and is suitable for pesticide development to control bacterial angular leaf spot of cucumber.
[0052] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.
Claims
1. A silicon-containing ring-rich pesticide composition, characterized in that, The pesticide composition uses silicone ring, copper quinoline, and synergistic extract as active ingredients; the synergistic extract includes at least one of grape seed extract and gentian extract.
2. The silicon-containing ring-rich pesticide composition according to claim 1, characterized in that, The grape seed extract is prepared by the following method: Grape seeds were collected, washed, dried in an oven at 60℃, and pulverized to pass through a 300-mesh sieve to obtain grape seed powder. The grape seed powder was extracted in 75% v / v ethanol at a mass-to-volume ratio of 1g:3ml at room temperature for 12 hours to obtain an extract. The solids after filtering to remove the extract were added to pure water at a mass-to-volume ratio of 1g:10ml and heated rapidly initially, maintaining a gentle boil for 2 hours. After heating was stopped, the mixture was allowed to stand for 40 minutes to remove the solids and aqueous phase, obtaining a secondary extract. The obtained extract and the secondary extract were combined and concentrated under reduced pressure at 60℃ to obtain the grape seed extract.
3. The silicon-containing ring-rich pesticide composition according to claim 1, characterized in that, The *Cymbidium ensifolium* extract is prepared by the following method: Take the whole flower of *Cymbidium faberi*, remove the calyx, wash and dry in an oven at 70℃, then pulverize to 300 mesh and sieve to obtain *Cymbidium faberi* powder; extract the *Cymbidium faberi* powder in 75% v / v ethanol at room temperature at a mass-to-volume ratio of 1g:2ml, each extraction time is 12h, and a total of 3 extractions are performed; combine the extracts obtained each time, concentrate under reduced pressure at 55℃, and then dry in an oven at 75℃ to obtain *Cymbidium faberi* extract.
4. The silicon-containing ring-rich pesticide composition according to claim 1, characterized in that, The synergistic extract is a mixture of grape seed extract and jasmine extract, wherein the ratio of grape seed extract to jasmine extract is (1-2)g:(1-2)g.
5. The silicon-containing ring-rich pesticide composition according to claim 1, characterized in that, The formulations of the pesticide compositions include suspensions, emulsions, microemulsions, or powders.
6. The silicon-containing ring-rich pesticide composition according to claim 1, characterized in that, The pesticide composition comprises the following components in parts by weight: 20-60 parts of silicone soluble powder, 20-60 parts of quinoline copper, and 2-10 parts of synergistic extract.
7. The silicon-containing ring-rich pesticide composition according to claim 6, characterized in that, In the silane-rich ring soluble powder, the content of silane-rich ring is not less than 50 wt%.
8. The silicon-containing ring-rich pesticide composition according to claim 6, characterized in that, In kasugamycin and quinoline copper, the sum of the contents of kasugamycin and quinoline copper is not less than 30 wt%.
9. The application of the silicon-containing ring-rich pesticide composition according to any one of claims 1-8 in the control of plant diseases, characterized in that, The plant diseases mentioned include bacterial angular leaf spot of cucumber.