One-step RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for detecting porcine getah disease virus infection and application thereof

By designing specific primer pairs and constructing a one-step RT-PCR detection kit, the problems of speed, specificity, sensitivity, and cost in GETV detection have been solved, achieving efficient detection of GETV, which is suitable for clinical sample detection and disease diagnosis of GETV.

CN122105009APending Publication Date: 2026-05-29CHINA INST OF VETERINARY DRUG CONTROL
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Patent Information

Application Number
CN202610352214.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-20
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

There is a lack of existing methods for detecting GETV that are rapid, specific, sensitive, and capable of qualitative detection, and GETV has a serious impact on economically important animals.

Method used

A set of specific primer pairs (GETV-F and GETV-R) was designed for RT-PCR detection. Combined with One Step Reaction Mix and TransScript® OneStep Enzyme Mix, a one-step RT-PCR detection kit was constructed, including positive and negative controls, to detect porcine Geytavirus via RT-PCR reaction.

Benefits of technology

It achieves rapid, specific, sensitive, simple and low-cost detection of GETV, can accurately quantify, and has no cross-reactivity with other swine, cattle and sheep pathogens, filling the gap in domestic GETV detection kits, and is widely used in clinical sample detection and disease diagnosis of GETV.

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Abstract

The invention provides a one-step RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for detecting porcine getah disease virus infection and application of the one-step RT-PCR detection kit, and belongs to the technical field of virus detection. The invention provides a one-step RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for detecting porcine Getah disease virus (GETV) infection and application thereof. The kit contains a pair of specific primers for detecting Getah disease virus. The kit disclosed by the invention is good in detection result specificity, and has no cross reaction with main pig disease members and common pig, cattle and sheep pathogens; the sensitivity is high, and the sensitivity of detecting a plasmid standard substance and the sensitivity of detecting GETV cytotoxicity are 5.9 copies and 0.3 TCID50 respectively; the detection is rapid, sensitive, simple and convenient, the cost is low, reverse transcription and PCR are carried out in one reaction tube, and pollution opportunities are reduced.
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