Primer probe composition, kit, method and use for enterovirus universal and ev71, ca6, ca10, ca16 detection
By designing primer-probe compositions and using qPCR and melting curve technology, a highly sensitive genotyping detection of enteroviruses, including EV71, CA6, CA10, and CA16, was achieved in a single tube. This solved the problems of incomplete genotyping and complex operation in existing technologies, and improved detection efficiency and accuracy.
CN122105010APending Publication Date: 2026-05-29BEIJING BOE TECH DEV CO LTD +1
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- BEIJING BOE TECH DEV CO LTD
- Filing Date
- 2026-03-26
- Publication Date
- 2026-05-29
Smart Images
Figure CN122105010A_ABST
Abstract
The application discloses primer probe composition, kit, method and use for enterovirus universal type and EV71, CA6, CA10, CA16 detection, which comprises the combination of upstream primers, probes and downstream primers for CA16, CA6, CA10, EVA71 and enterovirus universal type detection respectively. The application realizes the typing detection of enterovirus universal type and EV71, CA16, CA6, CA10 in one tube by designing specific probes and primers and combining qPCR+melt curve technology, and the system is provided with an internal standard, so that the extraction and amplification process can be monitored.
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