A hair-darkening essence containing active peptides and its preparation method

Abstract

This invention discloses a hair-darkening essence containing active peptides and its preparation method. The essence comprises a hair-darkening biomimetic peptide composition, a hair-darkening herbal extract, a softener, water, glycerin, a thickener, and a surfactant. The present invention first prepares a softener by copolymerizing N-vinylpyrrolidone, grafted monomers, and modified monomers, and then blends the softener with other basic raw materials to prepare the hair-darkening essence containing active peptides. The hair-darkening essence containing active peptides prepared by this invention has advantages such as good hair-darkening effect and effective improvement of hair smoothness and shine.

Description

Technical Field

[0001] This invention relates to the field of daily chemical product technology, and in particular to a hair-darkening essence containing active peptides and its preparation method. Background Technology

[0002] In today's pursuit of health and beauty, people's demands for hair care products are no longer limited to simple cleansing and moisturizing, but rather focus more on the scientific nature and effectiveness of their ingredients. Among these, a hair-darkening essence containing active peptides is an innovative hair care product that combines technology and natural essences.

[0003] Bioactive peptides, as an important class of bioactive substances in living organisms, are compounds composed of two or more amino acids linked by peptide bonds. They play a crucial role in human growth and development, metabolism, disease prevention and treatment, and delaying aging. Due to their unique biological activity and high permeability, bioactive peptides are widely used in health foods, cosmetics, and the medical field. In hair care products, bioactive peptides can penetrate deep into the scalp and hair shaft, providing rich nutrients to hair follicles and promoting healthy hair growth.

[0004] CN114209636A discloses a composition and its application in the preparation of daily chemical products with hair growth and / or hair darkening effects. The composition comprises Sargassum fusiforme extract and Alpinia galanga extract; wherein the weight ratio of Sargassum fusiforme extract to Alpinia galanga extract is 1-3:1; the composition also contains Sipunculus nudus tripeptide metal chelate; the composition has tyrosinase activating activity; therefore, this composition can be used as an active ingredient to develop products with hair darkening effects. By combining Sipunculus nudus tripeptide metal chelate, Sargassum fusiforme extract, and Alpinia galanga extract, it not only has excellent tyrosinase activating activity, thus achieving hair darkening, but also promotes hair growth. However, the hair darkening composition prepared by this method, using only extracts, is easily affected by environmental temperature and other factors, making it unstable and prone to affecting its effectiveness after prolonged storage.

[0005] CN109498469A discloses a walnut green husk hair dyeing and sunscreen spray, its preparation method, and its application. The formula of this walnut green husk hair dyeing and sunscreen spray is made entirely of natural plant ingredients, resulting in a mild and safe product. It causes no adverse irritation to hair and scalp, making it especially suitable for people who have dyed their hair black. It is primarily for hair that may have been exposed to sunlight for extended periods, enhancing the dyeing effect and providing sun protection, color fixing, and itch relief. It also makes hair soft and shiny, demonstrating good practical application value. However, this invention requires enzymatic hydrolysis and other processes, making the operation complex and susceptible to temperature and environmental factors. Summary of the Invention

[0006] In view of the above-mentioned deficiencies of the prior art, the technical problem to be solved by the present invention is to improve the hair darkening effect and make the hair shiny and smooth.

[0007] To achieve the above objectives, the present invention provides a hair-darkening essence containing active peptides, comprising, by weight, the following components: 5-10 parts of a hair-darkening biomimetic peptide composition, 2-8 parts of a hair-darkening traditional Chinese medicine extract, 5-10 parts of a softening agent, 20-40 parts of water, 1-5 parts of glycerin, 0.5-3 parts of a thickener, and 0.5-2 parts of a surfactant, wherein the softening agent is prepared from N-vinylpyrrolidone, a grafted monomer, a modified monomer, an initiator, and a crosslinking agent.

[0008] Preferably, the method for preparing the fabric softener includes the following steps: S1. Dissolve N-vinylpyrrolidone, graft monomer and modified monomer in solvent, heat to 50-90℃ in nitrogen atmosphere, add initiator, and then continuously add graft monomer over 3-4 hours, and stir for 1-3 hours to obtain copolymer. S2. Vacuum dry the copolymer for 20-30 hours, then grind it into powder to obtain the fabric softener.

[0009] More preferably, the method for preparing the fabric softener includes the following steps, in parts by weight: S1. Dissolve 20-40 parts of N-vinylpyrrolidone, 3-7 parts of graft monomer and 1-5 parts of modified monomer in a solvent, heat to 50-90℃ in a nitrogen atmosphere, add 0.1-1 parts of initiator, and then continuously add 2-4 parts of graft monomer within 3-4 hours, and stir for 1-3 hours to obtain the copolymer. S2. Vacuum dry the copolymer for 20-30 hours, then grind it into powder to obtain the fabric softener.

[0010] Preferably, the grafting monomer is any one of N-methyl-N-(1-oxo-2-propenyl)-β-alanine, N-(3-dimethylamino-2,2-dimethylpropyl)-2-methyl, and N-[3-[acryloyl-[3-(acryloylamino)propyl]amino]propyl]acrylamide.

[0011] Preferably, the modified monomer is any one of 1,3-dimethyl-1,1,3,3-tetraethylenedisiloxane, hexavinyldisiloxane, and 2-[methyl-bis(trimethylsiloxy)silyl]ethyl acrylate.

[0012] Preferably, the solvent is any one of ethanol, isopropanol, and ethylene glycol.

[0013] Preferably, the initiator is any one of azobisisobutyronitrile, benzoyl peroxide, acetyl peroxide, and dodecanoyl peroxide.

[0014] Preferably, the black hair biomimetic peptide composition is a mixture of palmitoyl dipeptide and palmitoyl tetrapeptide, with a mass ratio of palmitoyl dipeptide to palmitoyl tetrapeptide of (1-8):(1-10).

[0015] Preferably, the preparation method of the hair-darkening herbal extract includes the following steps, in parts by weight: Sift 1-4 parts of Platycladus orientalis leaves, 1-4 parts of Polygonum multiflorum, 1-3 parts of dried ginger, 1-2 parts of dandelion, 1-2 parts of loquat leaves, and 1-2 parts of licorice. Add 200-400 parts of 50-70wt% ethanol aqueous solution and soak at room temperature for 10-15 hours. Extract with ultrasound-assisted extraction for 10-20 minutes, filter, and reflux the filtrate in a water bath at 70-80℃ for 1-3 hours. Extract 1-3 times in total. Finally, concentrate under reduced pressure at 50-70℃ for 10-30 minutes to obtain the hair-darkening herbal extract.

[0016] A method for preparing a hair-darkening essence containing active peptides includes the following steps, in parts by weight: Step 1: Mix 20-40 parts water, 1-5 parts glycerin, and 0.5-2 parts surfactant, heat to 50-65℃, then add 5-10 parts hair-darkening biomimetic peptide composition, 2-8 parts hair-darkening herbal extract, and 5-10 parts conditioner, and stir at 1000-2500 rpm for 5-15 minutes to obtain a mixture. Step 2: Add 0.5-3 parts of thickener to the mixture, stir at 1000-2500 rpm for 10-20 minutes at 30-65℃, and cool to room temperature to obtain hair darkening essence containing active peptides.

[0017] In this formula, the ingredients and their functions are as follows: Hair-darkening biomimetic peptide composition: This composition mimics naturally occurring peptide signaling molecules related to melanin synthesis. By activating key enzymes such as tyrosinase in scalp cells, it promotes the proliferation of melanocytes and the synthesis of melanin, effectively improving gray hair caused by aging, stress, and environmental factors. Furthermore, these biomimetic peptides possess excellent biocompatibility and permeability, deeply nourishing the scalp and hair roots, providing essential nutritional support, promoting healthy hair growth, and resulting in darker, shinier hair with a natural shine.

[0018] Hair-darkening herbal extracts: Rich in various active ingredients such as alkaloids, flavonoids, polysaccharides, vitamins, and minerals. These ingredients deeply nourish the scalp, promote scalp blood circulation, and provide ample nutrition to hair follicles, thereby enhancing hair growth and health. Simultaneously, they possess antioxidant, anti-inflammatory, and antibacterial bioactivities, helping to improve the scalp environment, reduce dandruff, scalp itching, and other problems, while also contributing to hair darkening.

[0019] N-Vinylpyrrolidone: N-Vinylpyrrolidone belongs to the lactam class of substances and has properties similar to amides, exhibiting good compatibility. Its main function is to construct copolymer network structures and provide specific physicochemical properties. N-Vinylpyrrolidone is a vinyl monomer with excellent reactivity and film-forming properties. It can copolymerize with grafted and modified monomers under the action of an initiator to form high-molecular-weight copolymers. These copolymers not only possess good flexibility and elasticity but also form a uniform and durable protective film on the hair surface, helping to enhance hair styling and moisture resistance.

[0020] Grafted monomers: These molecules contain both tertiary amine groups and unsaturated double bonds, making them amides. They are highly reactive and can undergo copolymerization and quaternization reactions. These monomers can be used as comonomers with N-vinylpyrrolidone and modified monomers to prepare highly transparent and uniform fabric softeners with excellent softening and high-gloss properties.

[0021] Modifying monomers: The modifier is an organosiloxane compound. Organosiloxanes have excellent lubricity and film-forming properties, which can form a smooth and breathable protective film on the hair surface, reducing friction and static electricity between hair strands, making the hair smoother and easier to comb. At the same time, it can react with N-vinylpyrrolidone, grafted monomers, etc. during the copolymerization process to form a smoothing agent with excellent smoothing effect and high gloss properties.

[0022] Solvent: The solvent mainly plays the role of dissolving and dispersing the reactants, and is an indispensable part of the preparation process. First, the solvent can effectively dissolve reactants such as N-vinylpyrrolidone, grafted monomers, and modified monomers to form a homogeneous solution, providing the necessary reaction environment for subsequent polymerization reactions.

[0023] Water: Water provides a stable medium for the entire formula, allowing other ingredients such as the hair-darkening biomimetic peptide composition, hair-darkening herbal extracts, conditioning agents, glycerin, thickeners, and surfactants to disperse and dissolve evenly, forming a stable mixture. In addition, water has a moisturizing effect, helping hair maintain its moisture balance and reducing dryness and split ends.

[0024] Glycerin: First, glycerin has strong hygroscopic properties, absorbing moisture from the air to help keep hair hydrated and reduce frizz and split ends caused by dryness. Second, glycerin can penetrate the hair cuticle, forming a protective film that locks in moisture and prevents evaporation, thus enhancing the hair's moisturizing effect. In addition, glycerin has a certain lubricating property, improving the application of the serum and making it easier for the hair to absorb, while reducing damage caused by friction.

[0025] Thickeners: The main function of thickeners is to regulate and control the viscosity and flowability of the serum. By increasing the content of high molecular weight substances in the serum, thickeners form a three-dimensional network structure or associated structure, thereby effectively increasing the viscosity of the serum, making it easier to adhere to the hair, and providing long-lasting moisturizing and conditioning effects during use.

[0026] Surfactants: Surfactants can significantly reduce the surface tension between components, allowing active ingredients such as the hair-darkening biomimetic peptide composition, hair-darkening herbal extracts, and softeners to be uniformly and stably dispersed in the aqueous phase, avoiding aggregation and stratification between components, and ensuring the uniformity and stability of the serum.

[0027] The beneficial effects of this invention are: Compared with the prior art, the present invention modifies the polyamide polymer formed by copolymerizing N-vinylpyrrolidone and graft monomers with organosiloxane to prepare a smoothing agent with good film-forming feel, elasticity and luster. After the smoothing agent is blended with other hair darkening raw materials, the hair darkening essence containing active peptides can not only darken hair, but also make the hair have a natural texture, natural smoothness and luster. Detailed Implementation

[0028] The parameters and sources of the specific chemical substances used.

[0029] N-Methyl-N-(1-oxo-2-propenyl)-β-alanine, beta-Alanine, N-methyl-N-(1-oxo-2-propenyl)- (9CI), CAS No.: 658687-97-9.

[0030] N-(3-dimethylamino-2,2-dimethylpropyl)-2-methyl, N-[3-(dimethylamino)-2,2-dimethylpropyl]methacrylamide, CAS No.: 75150-23-1.

[0031] N-[3-[acryloyl-[3-(acryloylamino)propyl]amino]propyl]acrylamide, N-[3-[Prop-2-enoyl-[3-(prop-2-enoylamino)propyl]amino]propyl]prop-2-enamide, CAS No.: 143785-80-2.

[0032] 1,3-Dimethyl-1,1,3,3-tetraethylenedisiloxane, CAS No.: 16045-78-6.

[0033] 2-propenoic acid 2-[methyl-bis(trimethylsilyloxy)silyl]ethyl ester, CAS No.: 133726-21-3.

[0034] Carbomer 940, CAS No.: 9007-20-9.

[0035] Palmitoyl dipeptide-5, CAS No.: 794590-34-4.

[0036] Palmitoyl tetrapeptide-7, CAS No.: 221227-05-0.

[0037] Stearamide propyl dimethylamine, CAS No.: 20182-63-2.

[0038] Vinyl-terminated dimethyl polysiloxane, CAS No.: 68083-19-2.

[0039] Example 1 A method for preparing a hair-darkening essence containing active peptides includes the following steps: Step 1: Stir 40mL of water, 2g of glycerin, and 0.5g of stearamide propyl dimethylamine at 200rpm for 5min, heat to 60℃, then add 5g of palmitoyl dipeptide, 5g of palmitoyl tetrapeptide, 5g of hair-darkening herbal extract, and 8g of fabric softener, and stir at 1500rpm for 15min to obtain a mixture. Step 2: Add 1g of Carbomer 940 to the mixture, stir at 1500rpm for 20 minutes at 30℃, and cool to 25℃ to obtain the hair darkening essence containing active peptides.

[0040] The preparation method of the hair-darkening herbal extract includes the following steps: 400g of Platycladus orientalis leaves, 400g of Polygonum multiflorum, 200g of dried ginger, 100g of dandelion, 100g of loquat leaves, and 100g of licorice were passed through a 50-mesh sieve and added to 20L of 60wt% ethanol aqueous solution. The mixture was soaked at 25℃ for 15h and then extracted with 45kHz ultrasound-assisted extraction for 15min. The mixture was filtered and the filtrate was refluxed in a 75℃ water bath for 3h. The extraction was repeated twice. Finally, the mixture was concentrated under reduced pressure at 60℃ for 10min to obtain the hair-darkening herbal extract.

[0041] The method for preparing the fabric softener includes the following steps: S1. Dissolve 40g of N-vinylpyrrolidone, 5g of N-methyl-N-(1-oxo-2-propenyl)-β-alanine and 5g of 2-[methyl-bis(trimethylsiloxy)silyl]ethyl acrylate in 55mL of isopropanol, heat to 80℃ in a nitrogen atmosphere, add 1g of benzoyl peroxide, and then continuously add 4g of N-methyl-N-(1-oxo-2-propenyl)-β-alanine over 4h, and stir for another 2h to obtain the copolymer; S2. Dry the copolymer at 50°C for 24 hours, then grind it into powder to obtain the fabric softener.

[0042] Example 2 The difference between Example 2 and Example 1 is that N-methyl-N-(1-oxo-2-propenyl)-β-alanine in Example 1 is replaced with N-(3-dimethylamino-2,2-dimethylpropyl)-2-methyl.

[0043] Example 3 The difference between Example 3 and Example 1 is that N-methyl-N-(1-oxo-2-propenyl)-β-alanine in Example 1 is replaced with N-[3-[acryloyl-[3-(acryloylamino)propyl]amino]propyl]acrylamide.

[0044] Example 4 The difference between Example 4 and Example 1 is that 2-[methyl-bis(trimethylsiloxy)silyl]ethyl acrylate in Example 1 is replaced with 1,3-dimethyl-1,1,3,3-tetraethylenedisiloxane.

[0045] Example 5 The difference between Example 5 and Example 1 is that 2-[methyl-bis(trimethylsiloxy)silyl]ethyl acrylate in Example 1 is replaced with hexavinyldisiloxane.

[0046] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that N-methyl-N-(1-oxo-2-propenyl)-β-alanine in Example 1 is replaced with methacrylamide propyl dimethylamine.

[0047] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that 2-[methyl-bis(trimethylsiloxy)silyl]ethyl acrylate in Example 1 is replaced with vinyl-terminated dimethylpolysiloxane.

[0048] Test Example 1 Clinical trial results: 70 participants were tested to turn gray hair black, with an average of 10 people per group (healthy men or women), aged 20-45 years, with a total number of gray hairs ≤1000, and a blackening rate ≥70-90% to calculate the effectiveness. One cycle was 28 days, and three cycles constituted one course of treatment. After using the hair-darkening essence containing active peptides of this invention for a period of time, 65 people achieved the effect of turning their hair black, with a cure rate of 92.8%.

[0049] Instructions for use: When treating gray hair, randomly pluck out gray hairs and then apply 10mL of the hair-darkening essence produced in Examples 1-5 and Comparative Examples 1-2 to the plucked area twice a day (once in the morning and once in the evening, with an 8-hour interval). Note: (Plucking out gray hairs allows the hair follicles to be emptied, maximizing the absorption of the applied medication. The targeted action of the medication directly opens the nutrient channels of the hair follicle. External force helps replenish and synthesize melanin granules, allowing the empty hair follicle to regrow black hair.) Traditional Chinese medicine has numerous records of using medication after plucking gray hairs. Physiologically, melanocytes that secrete melanin are located at the bottom of the hair follicle; plucking out gray hairs facilitates the absorption and efficacy of active ingredients. Treatment is conducted in 28-day cycles, with three cycles constituting one course of treatment. Test results are shown in Table 1. Table 1 Results of Hair Darkening

[0050] Test Example 2 Combing performance test: 5g of untreated female hair bundles (30cm in length) were taken from three groups and immersed in the hair-darkening essence containing active peptides prepared in Examples 1-5 and Comparative Examples 1-2, respectively. The hair bundles were then combed to ensure no tangling, and hung vertically until no more hair-darkening essence dripped. They were then placed in a forced-air drying oven at 70℃ for 24 hours, removed, and placed in a constant temperature and humidity chamber at 25℃ and 90% relative humidity for 3 hours. Finally, the hair bundles were fixed to clips and combed using a comb from a tensile testing instrument. The maximum combing force was recorded, and the test was performed three times in parallel. The average value was taken as the combing force F1. The same method was used to measure the required combing force F0 for hair bundles not treated with the styling polymer. The reduction in combing force F1 / F0 was used to represent the quality of the copolymer's combing performance, and the percentage decrease in combing force was expressed as a percentage.

[0051] Gloss performance testing: A gloss tester from ERICHSEN TESTING EQUIPMENT (Germany) was used: Picogloss 20° / 60° / 85°, Model 503, with Picogloss 60° used for testing. All tests were conducted by applying 0.5 mL of product to a 2g strand of bleached hair, rubbing for three minutes, combing, rinsing with 50 mL of water, blow-drying, and then placing the hair in a constant temperature and humidity drying chamber for two hours before testing in the same environment. The gloss of the bleached hair (before shampooing) was defined as 1.0. The ratio of other test values ​​to this value was used as the gloss after use, and the result was expressed as the average of five gloss tests. Test results are shown in Table 2. Table 2 Results of Decreased Combing Power and Gloss

[0052] Test Example 3 Security performance testing: 1. Cytotoxicity Assay: HaCaT cells are a human immortalized epidermal cell line. Cytotoxicity test data for HaCaT cells can serve as a reference for skin safety. Logarithmically growing HaCaT cells are seeded into 96-well plates, 100 μL of cell suspension per well. The degree is 5×10 4 Cells were cultured at 37°C and 5% CO2 for 24 hours to allow them to adhere and grow. The cells in the 96-well plates were divided into 8 groups, corresponding to Examples 1-5 and Comparative Examples 1-2 (experimental groups) and a blank control, with 12 replicates per group. Old culture medium was removed, and the cells were washed with PBS. Hair-darkening essence containing active peptides, as provided in Examples 1-5 and Comparative Examples 1-2, was added to each well at a concentration of 0.1%. After culturing at 37°C and 5% CO2 for 48 hours, 10 μL of MTT (to a final concentration of 5 mg / mL) was added, and culturing continued for another 4 hours. The culture was terminated, the supernatant was discarded, and 50 μL of DMSO was added to each well. The plates were incubated for 10 minutes to allow the crystals to dissolve completely. The optical density (OD) value of each well was measured at 570 nm using a microplate reader. Cell viability was calculated as follows: (OD = ... 实验 –OD0) / (OD 对照 –OD0), where OD0 is the OD value of the culture medium.

[0053] 2. 3D Skin Irritation Test: Experimental Group: The hair-darkening essence containing active peptides provided in Examples 1-5 and Comparative Examples 1-2 were applied topically at a depth of approximately 0.38 cm. 2The skin model was exposed to PBS for 15 min; the negative control group was PBS, and the positive control group was SDS (sodium dodecyl sulfate), treated in the same way as the experimental group. After 15 min of contact, the skin model was rinsed with PBS and immersed in maintenance medium (2 mL) for 42 h under saturated humidity, 37 °C, and 5% CO2 conditions; then immersed in MTT solution (2 mL, 0.3 mg / mL, solvent is test medium) for 3 h under saturated humidity, 37 °C, and 5% CO2 conditions; after the MTT reaction, the skin model was immersed in a centrifuge tube containing 500 μL of acidified isopropanol, mixed well, and incubated overnight at room temperature in the dark. Then, 200 μL of liquid sample was taken from the centrifuge tube and placed in a 96-well plate. The OD value of each well was read at 570 nm using a microplate reader, with acidified isopropanol solution as a blank control; the average of the three test results was used to calculate the relative cell viability percentage = OD 实验 / OD 阴性对照 .

[0054] 3. Skin irritation of human patches: The hair-darkening essences containing active peptides provided in Examples 1-5 and Comparative Examples 1-2 were used for testing the skin irritation of human patches. 25 μL of the hair-darkening essence was dropped onto filter paper attached to the patch applicator and placed inside the applicator. The applicator containing the hair-darkening essence was then applied to the flexor side of the subject's forearm with non-irritating adhesive tape, and the palm was used to gently press it to ensure even application to the skin for 24 hours. Thirty minutes after removing the patch applicator, the skin reaction was observed after the pressure marks disappeared. If the result was negative, the skin reaction was observed again at 24 hours and 48 hours after the patch test. The skin reaction evaluation criteria for the closed patch test are shown in Table 3. Table 3 Skin Evaluation Criteria

[0055] An adverse skin reaction was defined as one in which more than 5 out of 30 subjects experienced a Grade 1 skin reaction, more than 2 out of 30 subjects experienced a Grade 2 skin reaction, or any one subject experienced a Grade 3 or higher skin reaction. 450 healthy volunteers aged 18-50 years were randomly divided into 15 groups and used the hair-darkening essence containing active peptides provided in Examples 1-5 and Comparative Examples 1-2, respectively. The scores of the closed patch test were recorded to obtain the results of the human patch skin irritation experiment.

[0056] The safety of the hair-darkening essences containing active peptides provided in Examples 1-5 and Comparative Examples 1-2 was tested according to the above experimental methods in terms of cytotoxicity, 3D skin irritation, and human patch skin irritation. The results are shown in Table 4. Table 4 Safety Performance Test

[0057] The hair-darkening compositions provided in Examples 1-5 and Comparative Examples 1-2 of this invention do not affect the growth and proliferation of HaCaT cells, are mild and non-irritating to 3D skin models, and exhibit high safety in human patch skin irritation experiments, proving that the hair-darkening compositions provided by this invention are mild and non-irritating to human skin, have no adverse reactions, and have good safety.

[0058] Based on the test data results in Table 2 of Examples 1-5 and Comparative Examples 1-2, it can be found that the hair darkening essence containing active peptides prepared in Example 1 has the best hair darkening effect, hair shine enhancement, and smoothness performance.

[0059] Comparing the tabular data in Examples 1-3 and Comparative Example 1, it was found that the hair-darkening essence containing active peptides in Example 1 showed differences in improving hair shine and combing performance. This difference may be due to the use of different grafting monomers. Grafting monomers are amides, highly reactive, and capable of copolymerization and quaternization reactions. They are used as comonomers with N-vinylpyrrolidone and modified monomers to prepare conditioning agents. The grafting monomers in Example 1 contain tertiary amine groups, unsaturated double bonds, and carboxylic acid functional groups. N-vinylpyrrolidone can copolymerize with grafting monomers containing carboxylic acid functional groups to obtain polymers with good shine, elasticity, and long-lasting smoothness, easy washing, and good water resistance. In contrast, the grafting monomers in Examples 2-3 and Comparative Example 1 only contain double bonds and tertiary amine groups. After quaternization of the tertiary amine groups, they are copolymerized with N-vinylpyrrolidone to form polyquaternary ammonium salts. The surface of hair fibers has an opposite charge to that of polyquaternary ammonium salts, and the modified conditioning agent facilitates deposition and adsorption on the hair, thus having a conditioning effect. Therefore, the hair-darkening essence containing active peptides prepared in Examples 2-3 and Comparative Example 1 had poor effects on improving hair shine and smoothness.

[0060] Comparing the tabular data in Examples 1, 4-5, and Comparative Example 2, it was found that the hair-darkening essences of each group showed differences in improving hair shine and combing performance. This may be due to the use of different modified monomers. The modified monomers are organosiloxane compounds that can react with N-vinylpyrrolidone, graft monomers, etc., during copolymerization to form a smoothing agent with excellent smoothing effect and high shine. The modified monomer in Example 1 contains ester bonds in addition to vinyl and siloxane bonds. The good molecular flexibility of ester groups may increase the chain segment mobility, thereby further improving the flexibility of the copolymer. Another possible reason is that siloxane bonds are longer than carbon-carbon bonds and have flatter bond angles, making the polymer chain very flexible and easy to rotate, which can provide a very low liquid surface tension to the silicone oil polymer. The modified monomers in Examples 4-5 and Comparative Example 2 do not contain ester bonds and cannot form ester-containing polymers with N-vinylpyrrolidone. This results in poor polymer macromolecular chain mobility and makes it less likely for the polymer molecules to rotate, thus leading to poor polymer molecular flexibility. Consequently, the prepared modified styling agents are less effective in improving hair shine and combing performance.

[0061] Observing the data in Table 1, it was found that the hair-darkening essences containing active peptides in each group showed differences in their hair-darkening effects. Comparing Examples 1-5 and Comparative Examples 1-2, Example 1 showed a more significant hair-darkening effect. This may be because the smoothing agent prepared in Example 1 is more effective in smoothing and enhancing hair shine. This protective film of the smoothing agent can smooth the hair surface, reduce frizz and static electricity, and make the hair smoother and easier to manage. Frizzy hair is more susceptible to damage from external factors (such as ultraviolet rays and chemicals), leading to fading or deterioration of hair quality, making it harder to maintain its black color. On the other hand, a smooth and easy-to-manage hair surface is more conducive to the even distribution and penetration of the herbal extracts, allowing the active ingredients to penetrate deeper into the hair shaft and achieve a better hair-darkening effect. The smoothing agent improves hair smoothness and enhances hair shine, making it easier for the hair-darkening herbal extracts and the hair-darkening biomimetic peptide composition to work on the hair; while the hair-darkening herbal extracts, through their unique bioactive components, further enhance hair health and color, resulting in a synergistic effect.

Claims

1. A hair-darkening essence containing active peptides, characterized in that, The product comprises the following components in parts by weight: 5-10 parts of a hair-darkening biomimetic peptide composition, 2-8 parts of a hair-darkening herbal extract, 5-10 parts of a softener, 20-40 parts of water, 1-5 parts of glycerin, 0.5-3 parts of a thickener, and 0.5-2 parts of a surfactant, wherein the softener is prepared from N-vinylpyrrolidone, a grafted monomer, a modified monomer, an initiator, and a crosslinking agent.

2. The hair-darkening essence containing active peptides as described in claim 1, characterized in that, The method for preparing the fabric softener includes the following steps: S1. Dissolve N-vinylpyrrolidone, graft monomer and modified monomer in solvent, heat to 50-90℃ in nitrogen atmosphere, add initiator, and then continuously add graft monomer over 3-4 hours, and stir for 1-3 hours to obtain copolymer. S2. Vacuum dry the copolymer for 20-30 hours, then grind it into powder to obtain the fabric softener.

3. The hair-darkening essence containing active peptides as described in claim 2, characterized in that, The method for preparing the fabric softener includes the following steps, in parts by weight: S1. Dissolve 20-40 parts of N-vinylpyrrolidone, 3-7 parts of graft monomer and 1-5 parts of modified monomer in a solvent, heat to 50-90℃ in a nitrogen atmosphere, add 0.1-1 parts of initiator, and then continuously add 2-4 parts of graft monomer within 3-4 hours, and stir for 1-3 hours to obtain the copolymer. S2. Vacuum dry the copolymer for 20-30 hours, then grind it into powder to obtain the fabric softener.

4. The hair-darkening essence containing active peptides as described in claim 1, characterized in that, The grafting monomer is any one of N-methyl-N-(1-oxo-2-propenyl)-β-alanine, N-(3-dimethylamino-2,2-dimethylpropyl)-2-methyl, and N-[3-[acryloyl-[3-(acryloylamino)propyl]amino]propyl]acrylamide.

5. The hair-darkening essence containing active peptides as described in claim 1, characterized in that, The modified monomer is any one of 1,3-dimethyl-1,1,3,3-tetraethylenedisiloxane, hexavinyldisiloxane, and 2-[methyl-bis(trimethylsiloxy)silyl]ethyl acrylate.

6. The hair-darkening essence containing active peptides as described in claim 2, characterized in that, The solvent is any one of ethanol, isopropanol, and ethylene glycol.

7. The hair-darkening essence containing active peptides as described in claim 1, characterized in that, The initiator is any one of azobisisobutyronitrile, benzoyl peroxide, acetyl peroxide, and dodecanoyl peroxide.

8. The hair-darkening essence containing active peptides as described in claim 1, characterized in that, The black hair biomimetic peptide composition is a mixture of palmitoyl dipeptide and palmitoyl tetrapeptide, with a mass ratio of palmitoyl dipeptide to palmitoyl tetrapeptide of (1-8):(1-10).

9. The hair-darkening essence containing active peptides as described in claim 1, characterized in that, The preparation method of the hair-darkening herbal extract includes the following steps, in parts by weight: Sift 1-4 parts of Platycladus orientalis leaves, 1-4 parts of Polygonum multiflorum, 1-3 parts of dried ginger, 1-2 parts of dandelion, 1-2 parts of loquat leaves, and 1-2 parts of licorice. Add 200-400 parts of 50-70wt% ethanol aqueous solution and soak at room temperature for 10-15 hours. Extract with ultrasound-assisted extraction for 10-20 minutes, filter, and reflux the filtrate in a water bath at 70-80℃ for 1-3 hours. Extract 1-3 times in total. Finally, concentrate under reduced pressure at 50-70℃ for 10-30 minutes to obtain the hair-darkening herbal extract.

10. A method for preparing a hair-darkening essence containing active peptides as described in any one of claims 1-9, characterized in that, The steps include the following, in parts by weight: Step 1: Mix 20-40 parts water, 1-5 parts glycerin, and 0.5-2 parts surfactant, heat to 50-65℃, then add 5-10 parts hair-darkening biomimetic peptide composition, 2-8 parts hair-darkening herbal extract, and 5-10 parts conditioner, and stir at 1000-2500 rpm for 5-15 minutes to obtain a mixture. Step 2: Add 0.5-3 parts of thickener to the mixture, stir at 1000-2500 rpm for 10-20 minutes at 30-65℃, and cool to room temperature to obtain hair darkening essence containing active peptides.

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