A compound formulation of active ingredients of Zhuang and Yao medicines and its preparation method

Abstract

This invention discloses a compound Zhuang-Yao medicine active ingredient synergistic formulation and preparation method, relating to the field of ethnic medicine compound preparations and extraction and preparation technology. The invention uses a scientific ratio of each medicinal ingredient, particularly strictly controlling the molar ratio of *Qianjinba* flavonoids from the Zhuang medicine *Qianjinba* to *Lingxiangcao* lactones from the Yao medicine *Lingxiangcao* at 3:2. This precise ratio helps maximize the synergistic effect between the active ingredients. Through this synergistic effect, the compound Zhuang-Yao medicine formulation can more effectively exert its medicinal effects, showing superior efficacy in treating specific diseases or improving health conditions. Furthermore, the addition of other medicinal materials such as *Niudali* and *Zoumatai* further enriches the variety of active ingredients and enhances the overall pharmacological effect.

Description

Technical Field

[0001] This invention relates to the field of compound preparations of ethnic medicines and their extraction and preparation technology, specifically to a compound Zhuang and Yao medicine active ingredient synergistic formulation and preparation method. Background Technology

[0002] In the field of ethnic medicine, Zhuang and Yao medicine, as an important part of traditional Chinese medicine, has a long history and rich practical experience. With their unique medicinal properties and effects, Zhuang and Yao medicines are widely used in folk medicine to treat various diseases and maintain health. With the development of modern science and technology, the research on the active ingredients in Zhuang and Yao medicines has gradually deepened. How to scientifically combine these active ingredients to maximize their efficacy has become a current research hotspot.

[0003] Traditional methods for extracting and compounding Zhuang and Yao medicines rely heavily on experience and manual operation, resulting in low extraction efficiency, significant loss of active ingredients, and unscientific compounding ratios. Specifically, traditional extraction processes often employ simple water decoction or alcohol extraction, which are not only time-consuming but also fail to adequately break down the cell walls of the medicinal materials, leading to incomplete release of active ingredients and low extraction rates. Furthermore, due to a lack of scientific basis for compounding, the proportions of each medicinal material in traditional formulas are often determined empirically, making it difficult to guarantee optimal synergistic effects between active ingredients and thus affecting the overall efficacy. In addition, traditional methods easily cause damage and loss of active ingredients during extraction, further reducing the utilization rate and therapeutic effect of the medicinal materials.

[0004] In view of the problems of low extraction efficiency, large loss of active ingredients, and unscientific compatibility ratio in traditional Zhuang and Yao medicine extraction and compatibility techniques, this invention proposes a compound Zhuang and Yao medicine active ingredient synergistic compatibility formula and preparation method, which is of particular importance. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a compound formulation and preparation method for the synergistic combination of active ingredients of Zhuang and Yao medicines. It can achieve the best synergistic effect between active ingredients by precisely controlling the ratio of each medicinal ingredient, especially the molar ratio of flavonoids in Zhuang medicine Qianjinba and ligustilide in Yao medicine Lingxiangcao. At the same time, the use of enzymatic pretreatment combined with ultrasonic-microwave synergistic extraction technology significantly improves the extraction efficiency of active ingredients and preserves the effective components in the medicinal materials.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a compound formula of active ingredients of Zhuang and Yao medicine, which is made from the following raw materials in parts by weight:

[0007] The ingredients are 15-20 parts of the Zhuang medicine *Qianjinba*, 10-15 parts of the Yao medicine *Lingxiangcao*, 8-12 parts of the Zhuang medicine *Niudali*, and 6-10 parts of the Yao medicine *Zoumatai*; and the molar ratio of *Qianjinba* flavonoids contained in the Zhuang medicine *Qianjinba* to *Lingxiangcao* lactones contained in the Yao medicine *Lingxiangcao* is 3:2.

[0008] Furthermore, the medicinal herb *Qianjinba* is the dried root of *Qianjinba*, a legume.

[0009] Furthermore, the Yao medicine Lingxiangcao is the dried whole herb of Lingxiangcao, a plant of the Primulaceae family.

[0010] Furthermore, the content of *Niu Dali* polysaccharide in the Zhuang medicine *Niu Dali* is ≥10.0%, calculated on a dry basis.

[0011] Furthermore, the medicinal materials need to be pulverized to 80-100 mesh before being mixed.

[0012] On the other hand, a method for preparing a compound Zhuang-Yao medicine active ingredient synergistic formulation is characterized by the following steps:

[0013] S1. Pre-treat each medicinal material in the formula to obtain medicinal powder;

[0014] S2. Add cellulase and pectinase to the medicinal powder for enzymatic pretreatment to obtain enzymatic hydrolysate;

[0015] S3. Extract the enzymatic hydrolysate using a combination of ultrasound and microwave to obtain the initial extract.

[0016] Furthermore, in step S2, the cellulase activity is ≥5000 U / g, and the pectinase activity is ≥3000 U / g.

[0017] Furthermore, in step S2, the mass of deionized water added to the medicinal powder is 10-15 times the mass of the medicinal powder.

[0018] Furthermore, in step S3, the ultrasonic power is 200-250W and the microwave power is 300-350W.

[0019] Furthermore, the method also includes step S4, filtering the initial extract through a 0.22μm microporous membrane to obtain a clarified liquid containing the active ingredients of the compound Zhuang and Yao medicines.

[0020] Compared with existing technologies, this compound Zhuang-Yao medicine active ingredient synergistic formulation and preparation method have the following beneficial effects:

[0021] I. This invention utilizes a scientifically proportioned formula of various medicinal ingredients. In particular, the molar ratio of flavonoids from the Zhuang medicine *Qianjinba* to ligustrol from the Yao medicine *Lingxiangcao* is strictly controlled at 3:2. This precise ratio helps to maximize the synergistic effect between the active ingredients. Through this synergistic effect, the compound Zhuang and Yao medicine formula can more effectively exert its medicinal effects and show superior results in treating specific diseases or improving health conditions. In addition, the addition of other medicinal materials such as *Niudali* and *Zoumatai* further enriches the variety of active ingredients and enhances the overall pharmacological effect.

[0022] II. The preparation method of the present invention significantly improves the extraction efficiency of active ingredients in Zhuang and Yao medicines by combining enzymatic pretreatment with ultrasonic-microwave synergistic extraction technology. Specifically, the enzymatic hydrolysis process can destroy the cell wall of the medicinal material and release more active ingredients; while ultrasonic-microwave synergistic extraction further accelerates the dissolution of active ingredients through physical action, thereby obtaining a high-concentration initial extract in a short time. Compared with traditional extraction processes, this method greatly improves the extraction efficiency and helps to retain the effective components in the medicinal materials.

[0023] Other advantages, objectives and features of the invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination or study, or may be learned from the practice of the invention. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0025] Figure 1 A flowchart illustrating the synergistic formulation and preparation method of a compound Zhuang-Yao medicine active ingredient. Detailed Implementation

[0026] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with the accompanying drawings and preferred embodiments, is provided below.

[0027] Examples one to three only changed the content of the herb *Smilax glabra*.

[0028] Example 1

[0029] By weight, the formula of this embodiment is made from the following raw materials: 15 parts of Zhuang medicine Qianjinba, 12 parts of Yao medicine Lingxiangcao, 10 parts of Zhuang medicine Niudali, and 8 parts of Yao medicine Zoumatai; wherein, the molar ratio of Qianjinba flavonoids contained in Zhuang medicine Qianjinba to Lingxiangcao lactone contained in Yao medicine Lingxiangcao is precisely controlled to be 3:2.

[0030] The Zhuang medicine *Qianjinba* is the dried root of *Qianjinba*, a legume. Thin-layer chromatography analysis showed that its *Qianjinba* flavonoid content met the molar ratio requirements. The Yao medicine *Lingxiangcao* is the dried whole herb of *Lingxiangcao*, a plant in the Primulaceae family. High-performance liquid chromatography analysis showed that its *Lingxiangcao* lactone content met the aforementioned molar ratio. The Zhuang medicine *Niudali* is a dried tuberous root conforming to pharmacopoeia standards. On a dry basis, its *Niudali* polysaccharide content is 10.5%, ≥ the 10.0% limit specified in the claims, ensuring the basic content of the active ingredients in the raw materials.

[0031] This embodiment employs a preparation process combining enzymatic pretreatment with ultrasonic-microwave synergistic extraction. The specific steps are as follows:

[0032] The above-mentioned proportions of Zhuang medicinal herbs *Qianjinba*, Yao medicinal herbs *Lingxiangcao*, Zhuang medicinal herbs *Niudali*, and Yao medicinal herbs *Zoumatai* were each purified to remove impurities, mud, and non-medicinal parts. They were then dried in a 60℃ hot air drying oven until constant weight. After drying, each herb was separately fed into a universal pulverizer and pulverized, then passed through a 90-mesh standard sieve to obtain herb powder. The herb powders were then thoroughly mixed according to the formula ratio to obtain a mixed herb powder. This step of pulverizing the herbs to 90 mesh (within the 80-100 mesh range) increases the contact area between the herbs and the extraction solvent, improving subsequent extraction efficiency, while avoiding problems such as difficulty in filtration of the extract and excessive dissolution of impurities caused by excessive pulverization.

[0033] Take 1 kg of the above mixed medicinal powder, add 12 times its weight of deionized water, stir well, and adjust the pH of the system to 5.0. Then add cellulase and pectinase, where the cellulase activity is 5000 U / g and the amount added is 0.5% of the weight of the mixed medicinal powder, and the pectinase activity is 3000 U / g and the amount added is 0.3% of the weight of the mixed medicinal powder. Place the mixture in a 45℃ constant temperature water bath shaker and shake at 150 r / min for 2 hours for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, place the system in a 95℃ water bath for 10 minutes to inactivate the enzyme. After cooling to room temperature, the enzymatic hydrolysate is obtained. Cellulase can break down cellulose in the cell walls of medicinal materials, while pectinase can break down pectin. The synergistic effect of the two can effectively destroy the cell wall structure of medicinal materials, breaking the binding state between active ingredients and the cell wall, thus creating conditions for the dissolution of active ingredients in subsequent extraction steps. Compared with the process without enzymatic pretreatment, the dissolution rate of active ingredients can be increased by more than 20%. Enzyme inactivation treatment can avoid the continuous action of enzymes that would damage the structure of active ingredients, ensuring the stability of active ingredients.

[0034] The enzymatic hydrolysate was transferred to an ultrasonic-microwave synergistic extraction device. Extraction parameters were set as follows: ultrasonic power 220W, microwave power 320W, extraction temperature 60℃, and extraction time 30min. Stirring was continuous during extraction. After extraction, ultrasonic and microwave action was stopped. The system was allowed to cool to room temperature, and the residue was removed by filtration to obtain the initial extract. Ultrasonic-microwave synergistic extraction combines the cavitation effect of ultrasound with the thermal effect of microwaves. Ultrasonic cavitation creates tiny bubbles in the solvent that burst instantly, generating localized high pressure and accelerating the dissolution of active ingredients. Microwave thermal effect causes rapid vibration of solvent molecules, achieving uniform heating of the system and shortening the extraction time. In this embodiment, the ultrasonic power (200-250W) and microwave power (300-350W) ranged from 220W to 320W, ensuring extraction efficiency while avoiding problems such as rapid solvent evaporation and thermal degradation of active ingredients caused by excessively high power. Compared with traditional reflux extraction, the extraction time was shortened by 60%, and the retention rate of active ingredients was increased by 15%.

[0035] The initial extract was pressure filtered through a 0.22μm microporous membrane at a controlled pressure of 0.1MPa. After filtration, the filtrate was collected, yielding a clear liquid containing the active ingredients of the Zhuang and Yao medicinal herbs. Using a 0.22μm microporous membrane effectively removes tiny suspended particles, colloidal impurities, and microorganisms from the initial extract, resulting in a clear and transparent filtrate. Simultaneously, it does not retain small-molecule active ingredients from the Zhuang and Yao herbs, ensuring the content of these active ingredients. Pressure filtration further improves filtration efficiency, making it suitable for industrial production applications.

[0036] Results: Dissolution rate of active ingredients: The dissolution rate of flavonoids from *Millettia dielsiana* reached 90.1%, ginsenosides reached 91.5%, and polysaccharides from *Millettia dielsiana* reached 88.9%, which were improved by 21.9%, 21.0%, and 23.9% respectively compared with the traditional ethanol reflux extraction process;

[0037] Retention rate of active ingredients: After enzymatic hydrolysis and ultrasonic-microwave synergistic extraction, the retention rates of *Millettia dielsiana* flavonoids, *Gynostemma pentaphyllum* lactone, and *Achyranthes bidentata* polysaccharide were 98.1%, 97.5%, and 96.7%, respectively; after microfiltration, the retention rates were 97.8%, 97.1%, and 96.4%, respectively, with a decrease of less than 0.5%.

[0038] Impurity removal and clarification effects: The removal rate of suspended particles reached 99.9%, and the transmittance of the clarified liquid reached 99.0%; the microbial removal rate reached 100%, with bacterial, mold, and yeast counts all <10 CFU / mL, and Escherichia coli not detected; the removal rate of macromolecular impurities reached 85.5%.

[0039] Synergistic biological effects: The clarified solution with a mass concentration of 1 mg / mL achieved a DPPH free radical scavenging rate of 85.3% and a half-maximal inhibitory concentration (IC50) of 1 mg / mL. 50The concentration was 0.41 mg / mL; the clarified solution with a mass concentration of 0.5 mg / mL inhibited the NO release of LPS-induced RAW264.7 macrophages by 72.4%, and reduced the NO release to 34.8 μmol / L.

[0040] Process efficiency: Extraction time is 30 min, which is 93.75% shorter than the traditional process; solvent consumption is reduced by 60% and unit energy consumption is reduced by 45.2%.

[0041] Example 2

[0042] By weight, the formula of this embodiment is made from the following raw materials: 17 parts of Zhuang medicine Qianjinba, 12 parts of Yao medicine Lingxiangcao, 10 parts of Zhuang medicine Niudali, and 8 parts of Yao medicine Zoumatai; wherein, the molar ratio of Qianjinba flavonoids contained in Zhuang medicine Qianjinba to Lingxiangcao lactone contained in Yao medicine Lingxiangcao is precisely controlled at 3:2.

[0043] The Zhuang medicine *Qianjinba* is the dried root of *Qianjinba*, a legume. Thin-layer chromatography analysis showed that its *Qianjinba* flavonoid content met the molar ratio requirements. The Yao medicine *Lingxiangcao* is the dried whole herb of *Lingxiangcao*, a plant in the Primulaceae family. High-performance liquid chromatography analysis showed that its *Lingxiangcao* lactone content met the aforementioned molar ratio. The Zhuang medicine *Niudali* is a dried tuberous root conforming to pharmacopoeia standards. On a dry basis, its *Niudali* polysaccharide content is 10.5%, ≥ the 10.0% limit specified in the claims, ensuring the basic content of the active ingredients in the raw materials.

[0044] This embodiment employs a preparation process combining enzymatic pretreatment with ultrasonic-microwave synergistic extraction. The specific steps are as follows:

[0045] The above-mentioned proportions of Zhuang medicinal herbs *Qianjinba*, Yao medicinal herbs *Lingxiangcao*, Zhuang medicinal herbs *Niudali*, and Yao medicinal herbs *Zoumatai* were each purified to remove impurities, mud, and non-medicinal parts. They were then dried in a 60℃ hot air drying oven until constant weight. After drying, each herb was separately fed into a universal pulverizer and pulverized, then passed through a 90-mesh standard sieve to obtain herb powder. The herb powders were then thoroughly mixed according to the formula ratio to obtain a mixed herb powder. This step of pulverizing the herbs to 90 mesh (within the 80-100 mesh range) increases the contact area between the herbs and the extraction solvent, improving subsequent extraction efficiency, while avoiding problems such as difficulty in filtration of the extract and excessive dissolution of impurities caused by excessive pulverization.

[0046] Take 1 kg of the above mixed medicinal powder, add 12 times its weight of deionized water, stir well, and adjust the pH of the system to 5.0. Then add cellulase and pectinase, where the cellulase activity is 5000 U / g and the amount added is 0.5% of the weight of the mixed medicinal powder, and the pectinase activity is 3000 U / g and the amount added is 0.3% of the weight of the mixed medicinal powder. Place the mixture in a 45℃ constant temperature water bath shaker and shake at 150 r / min for 2 hours for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, place the system in a 95℃ water bath for 10 minutes to inactivate the enzyme. After cooling to room temperature, the enzymatic hydrolysate is obtained. Cellulase can break down cellulose in the cell walls of medicinal materials, while pectinase can break down pectin. The synergistic effect of the two can effectively destroy the cell wall structure of medicinal materials, breaking the binding state between active ingredients and the cell wall, thus creating conditions for the dissolution of active ingredients in subsequent extraction steps. Compared with the process without enzymatic pretreatment, the dissolution rate of active ingredients can be increased by more than 20%. Enzyme inactivation treatment can avoid the continuous action of enzymes that would damage the structure of active ingredients, ensuring the stability of active ingredients.

[0047] The enzymatic hydrolysate was transferred to an ultrasonic-microwave synergistic extraction device. Extraction parameters were set as follows: ultrasonic power 220W, microwave power 320W, extraction temperature 60℃, and extraction time 30min. Stirring was continuous during extraction. After extraction, ultrasonic and microwave action was stopped. The system was allowed to cool to room temperature, and the residue was removed by filtration to obtain the initial extract. Ultrasonic-microwave synergistic extraction combines the cavitation effect of ultrasound with the thermal effect of microwaves. Ultrasonic cavitation creates tiny bubbles in the solvent that burst instantly, generating localized high pressure and accelerating the dissolution of active ingredients. Microwave thermal effect causes rapid vibration of solvent molecules, achieving uniform heating of the system and shortening the extraction time. In this embodiment, the ultrasonic power (200-250W) and microwave power (300-350W) ranged from 220W to 320W, ensuring extraction efficiency while avoiding problems such as rapid solvent evaporation and thermal degradation of active ingredients caused by excessively high power. Compared with traditional reflux extraction, the extraction time was shortened by 60%, and the retention rate of active ingredients was increased by 15%.

[0048] The initial extract was pressure filtered through a 0.22μm microporous membrane at a controlled pressure of 0.1MPa. After filtration, the filtrate was collected, yielding a clear liquid containing the active ingredients of the Zhuang and Yao medicinal herbs. Using a 0.22μm microporous membrane effectively removes tiny suspended particles, colloidal impurities, and microorganisms from the initial extract, resulting in a clear and transparent filtrate. Simultaneously, it does not retain small-molecule active ingredients from the Zhuang and Yao herbs, ensuring the content of these active ingredients. Pressure filtration further improves filtration efficiency, making it suitable for industrial production applications.

[0049] Results: Dissolution rate of active ingredients: The dissolution rate of flavonoids from *Millettia dielsiana* reached 91.8%, ginsenosides reached 91.7%, and polysaccharides from *Millettia dielsiana* reached 89.0%, which were improved by 23.6%, 21.2%, and 24.0% respectively compared with the traditional ethanol reflux extraction process;

[0050] Retention rate of active ingredients: After enzymatic hydrolysis and ultrasonic-microwave synergistic extraction, the retention rates of *Millettia dielsiana* flavonoids, *Gynostemma pentaphyllum* lactone, and *Achyranthes bidentata* polysaccharide were 98.2%, 97.6%, and 96.8%, respectively; after microfiltration, the retention rates were 97.9%, 97.2%, and 96.5%, respectively, with a decrease of less than 0.5%.

[0051] Impurity removal and clarification effects: The removal rate of suspended particles reached 99.9%, and the transmittance of the clarified liquid reached 99.1%; the microbial removal rate reached 100%, with bacterial, mold, and yeast counts all <10 CFU / mL, and Escherichia coli not detected; the removal rate of macromolecular impurities reached 85.6%;

[0052] Synergistic biological effects: The clarified solution with a mass concentration of 1 mg / mL achieved a DPPH free radical scavenging rate of 88.7% and a half-maximal inhibitory concentration (IC50) of 1 mg / mL. 50 The concentration was 0.36 mg / mL; the clarified solution with a mass concentration of 0.5 mg / mL inhibited the NO release of LPS-induced RAW264.7 macrophages by 76.2%, and reduced the NO release to 29.5 μmol / L.

[0053] Process efficiency: Extraction time is 30 min, which is 93.75% shorter than the traditional process; solvent consumption is reduced by 60% and unit energy consumption is reduced by 45.2%.

[0054] Example 3

[0055] By weight, the formula of this embodiment is made from the following raw materials: 20 parts of Zhuang medicine Qianjinba, 12 parts of Yao medicine Lingxiangcao, 10 parts of Zhuang medicine Niudali, and 8 parts of Yao medicine Zoumatai; wherein, the molar ratio of Qianjinba flavonoids contained in Zhuang medicine Qianjinba to Lingxiangcao lactone contained in Yao medicine Lingxiangcao is precisely controlled at 3:2.

[0056] The Zhuang medicine *Qianjinba* is the dried root of *Qianjinba*, a legume. Thin-layer chromatography analysis showed that its *Qianjinba* flavonoid content met the molar ratio requirements. The Yao medicine *Lingxiangcao* is the dried whole herb of *Lingxiangcao*, a plant in the Primulaceae family. High-performance liquid chromatography analysis showed that its *Lingxiangcao* lactone content met the aforementioned molar ratio. The Zhuang medicine *Niudali* is a dried tuberous root conforming to pharmacopoeia standards. On a dry basis, its *Niudali* polysaccharide content is 10.5%, ≥ the 10.0% limit specified in the claims, ensuring the basic content of the active ingredients in the raw materials.

[0057] This embodiment employs a preparation process combining enzymatic pretreatment with ultrasonic-microwave synergistic extraction. The specific steps are as follows:

[0058] The above-mentioned proportions of Zhuang medicinal herbs *Qianjinba*, Yao medicinal herbs *Lingxiangcao*, Zhuang medicinal herbs *Niudali*, and Yao medicinal herbs *Zoumatai* were each purified to remove impurities, mud, and non-medicinal parts. They were then dried in a 60℃ hot air drying oven until constant weight. After drying, each herb was separately fed into a universal pulverizer and pulverized, then passed through a 90-mesh standard sieve to obtain herb powder. The herb powders were then thoroughly mixed according to the formula ratio to obtain a mixed herb powder. This step of pulverizing the herbs to 90 mesh (within the 80-100 mesh range) increases the contact area between the herbs and the extraction solvent, improving subsequent extraction efficiency, while avoiding problems such as difficulty in filtration of the extract and excessive dissolution of impurities caused by excessive pulverization.

[0059] Take 1 kg of the above mixed medicinal powder, add 12 times its weight of deionized water, stir well, and adjust the pH of the system to 5.0. Then add cellulase and pectinase, where the cellulase activity is 5000 U / g and the amount added is 0.5% of the weight of the mixed medicinal powder, and the pectinase activity is 3000 U / g and the amount added is 0.3% of the weight of the mixed medicinal powder. Place the mixture in a 45℃ constant temperature water bath shaker and shake at 150 r / min for 2 hours for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, place the system in a 95℃ water bath for 10 minutes to inactivate the enzyme. After cooling to room temperature, the enzymatic hydrolysate is obtained. Cellulase can break down cellulose in the cell walls of medicinal materials, while pectinase can break down pectin. The synergistic effect of the two can effectively destroy the cell wall structure of medicinal materials, breaking the binding state between active ingredients and the cell wall, thus creating conditions for the dissolution of active ingredients in subsequent extraction steps. Compared with the process without enzymatic pretreatment, the dissolution rate of active ingredients can be increased by more than 20%. Enzyme inactivation treatment can avoid the continuous action of enzymes that would damage the structure of active ingredients, ensuring the stability of active ingredients.

[0060] The enzymatic hydrolysate was transferred to an ultrasonic-microwave synergistic extraction device. Extraction parameters were set as follows: ultrasonic power 220W, microwave power 320W, extraction temperature 60℃, and extraction time 30min. Stirring was continuous during extraction. After extraction, ultrasonic and microwave action was stopped. The system was allowed to cool to room temperature, and the residue was removed by filtration to obtain the initial extract. Ultrasonic-microwave synergistic extraction combines the cavitation effect of ultrasound with the thermal effect of microwaves. Ultrasonic cavitation creates tiny bubbles in the solvent that burst instantly, generating localized high pressure and accelerating the dissolution of active ingredients. Microwave thermal effect causes rapid vibration of solvent molecules, achieving uniform heating of the system and shortening the extraction time. In this embodiment, the ultrasonic power (200-250W) and microwave power (300-350W) ranged from 220W to 320W, ensuring extraction efficiency while avoiding problems such as rapid solvent evaporation and thermal degradation of active ingredients caused by excessively high power. Compared with traditional reflux extraction, the extraction time was shortened by 60%, and the retention rate of active ingredients was increased by 15%.

[0061] The initial extract was pressure filtered through a 0.22μm microporous membrane at a controlled pressure of 0.1MPa. After filtration, the filtrate was collected, yielding a clear liquid containing the active ingredients of the Zhuang and Yao medicinal herbs. Using a 0.22μm microporous membrane effectively removes tiny suspended particles, colloidal impurities, and microorganisms from the initial extract, resulting in a clear and transparent filtrate. Simultaneously, it does not retain small-molecule active ingredients from the Zhuang and Yao herbs, ensuring the content of these active ingredients. Pressure filtration further improves filtration efficiency, making it suitable for industrial production applications.

[0062] Results: Dissolution rate of active ingredients: The dissolution rate of flavonoids from *Millettia dielsiana* reached 94.2%, ginsenosides reached 92.1%, and polysaccharides from *Millettia dielsiana* reached 89.5%, which were improved by 26.0%, 21.6%, and 24.5% respectively compared with the traditional ethanol reflux extraction process;

[0063] Retention rate of active ingredients: After enzymatic hydrolysis and ultrasonic-microwave synergistic extraction, the retention rates of *Millettia dielsiana* flavonoids, *Gynostemma pentaphyllum* lactone, and *Achyranthes bidentata* polysaccharide were 98.4%, 97.7%, and 96.9%, respectively; after microfiltration, the retention rates were 98.0%, 97.3%, and 96.6%, respectively, with a decrease of less than 0.5% in all cases.

[0064] Impurity removal and clarification effects: The removal rate of suspended particles reached 99.9%, and the transmittance of the clarified liquid reached 99.3%; the microbial removal rate reached 100%, with bacterial, mold, and yeast counts all <10 CFU / mL, and Escherichia coli not detected; the removal rate of macromolecular impurities reached 85.8%.

[0065] Synergistic biological effects: The clarified solution with a mass concentration of 1 mg / mL achieved a DPPH free radical scavenging rate of 93.5% and a half-maximal inhibitory concentration (IC50) of 1 mg / mL. 50 The concentration was 0.28 mg / mL; the clarified solution with a mass concentration of 0.5 mg / mL inhibited the NO release of LPS-induced RAW264.7 macrophages by 82.7%, and reduced the NO release to 22.6 μmol / L.

[0066] Process efficiency: Extraction time is 30 min, which is 93.75% shorter than the traditional process; solvent consumption is reduced by 60% and unit energy consumption is reduced by 45.2%.

[0067] Examples 4 to 6 only changed the content of the Yao herb Lingxiang.

[0068] Example 4

[0069] By weight, the formula of this embodiment is made from the following raw materials: 18 parts of Zhuang medicine Qianjinba, 10 parts of Yao medicine Lingxiangcao, 10 parts of Zhuang medicine Niudali, and 8 parts of Yao medicine Zoumatai; wherein, the molar ratio of Qianjinba flavonoids contained in Zhuang medicine Qianjinba to Lingxiangcao lactone contained in Yao medicine Lingxiangcao is precisely controlled at 3:2.

[0070] The Zhuang medicine *Qianjinba* is the dried root of *Qianjinba*, a legume. Thin-layer chromatography analysis showed that its *Qianjinba* flavonoid content met the molar ratio requirements. The Yao medicine *Lingxiangcao* is the dried whole herb of *Lingxiangcao*, a plant in the Primulaceae family. High-performance liquid chromatography analysis showed that its *Lingxiangcao* lactone content met the aforementioned molar ratio. The Zhuang medicine *Niudali* is a dried tuberous root conforming to pharmacopoeia standards. On a dry basis, its *Niudali* polysaccharide content is 10.5%, ≥ the 10.0% limit specified in the claims, ensuring the basic content of the active ingredients in the raw materials.

[0071] This embodiment employs a preparation process combining enzymatic pretreatment with ultrasonic-microwave synergistic extraction. The specific steps are as follows:

[0072] The above-mentioned proportions of Zhuang medicinal herbs *Qianjinba*, Yao medicinal herbs *Lingxiangcao*, Zhuang medicinal herbs *Niudali*, and Yao medicinal herbs *Zoumatai* were each purified to remove impurities, mud, and non-medicinal parts. They were then dried in a 60℃ hot air drying oven until constant weight. After drying, each herb was separately fed into a universal pulverizer and pulverized, then passed through a 90-mesh standard sieve to obtain herb powder. The herb powders were then thoroughly mixed according to the formula ratio to obtain a mixed herb powder. This step of pulverizing the herbs to 90 mesh (within the 80-100 mesh range) increases the contact area between the herbs and the extraction solvent, improving subsequent extraction efficiency, while avoiding problems such as difficulty in filtration of the extract and excessive dissolution of impurities caused by excessive pulverization.

[0073] Take 1 kg of the above mixed medicinal powder, add 12 times its weight of deionized water, stir well, and adjust the pH of the system to 5.0. Then add cellulase and pectinase, where the cellulase activity is 5000 U / g and the amount added is 0.5% of the weight of the mixed medicinal powder, and the pectinase activity is 3000 U / g and the amount added is 0.3% of the weight of the mixed medicinal powder. Place the mixture in a 45℃ constant temperature water bath shaker and shake at 150 r / min for 2 hours for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, place the system in a 95℃ water bath for 10 minutes to inactivate the enzyme. After cooling to room temperature, the enzymatic hydrolysate is obtained. Cellulase can break down cellulose in the cell walls of medicinal materials, while pectinase can break down pectin. The synergistic effect of the two can effectively destroy the cell wall structure of medicinal materials, breaking the binding state between active ingredients and the cell wall, thus creating conditions for the dissolution of active ingredients in subsequent extraction steps. Compared with the process without enzymatic pretreatment, the dissolution rate of active ingredients can be increased by more than 20%. Enzyme inactivation treatment can avoid the continuous action of enzymes that would damage the structure of active ingredients, ensuring the stability of active ingredients.

[0074] The enzymatic hydrolysate was transferred to an ultrasonic-microwave synergistic extraction device. Extraction parameters were set as follows: ultrasonic power 220W, microwave power 320W, extraction temperature 60℃, and extraction time 30min. Stirring was continuous during extraction. After extraction, ultrasonic and microwave action was stopped. The system was allowed to cool to room temperature, and the residue was removed by filtration to obtain the initial extract. Ultrasonic-microwave synergistic extraction combines the cavitation effect of ultrasound with the thermal effect of microwaves. Ultrasonic cavitation creates tiny bubbles in the solvent that burst instantly, generating localized high pressure and accelerating the dissolution of active ingredients. Microwave thermal effect causes rapid vibration of solvent molecules, achieving uniform heating of the system and shortening the extraction time. In this embodiment, the ultrasonic power (200-250W) and microwave power (300-350W) ranged from 220W to 320W, ensuring extraction efficiency while avoiding problems such as rapid solvent evaporation and thermal degradation of active ingredients caused by excessively high power. Compared with traditional reflux extraction, the extraction time was shortened by 60%, and the retention rate of active ingredients was increased by 15%.

[0075] The initial extract was pressure filtered through a 0.22μm microporous membrane at a controlled pressure of 0.1MPa. After filtration, the filtrate was collected, yielding a clear liquid containing the active ingredients of the Zhuang and Yao medicinal herbs. Using a 0.22μm microporous membrane effectively removes tiny suspended particles, colloidal impurities, and microorganisms from the initial extract, resulting in a clear and transparent filtrate. Simultaneously, it does not retain small-molecule active ingredients from the Zhuang and Yao herbs, ensuring the content of these active ingredients. Pressure filtration further improves filtration efficiency, making it suitable for industrial production applications.

[0076] Results: Dissolution rate of active ingredients: The dissolution rate of flavonoids from *Millettia dielsiana* reached 92.3%, ginsenosides reached 90.2%, and polysaccharides from *Millettia dielsiana* reached 89.0%, which were improved by 24.1%, 19.7%, and 24.0% respectively compared with the traditional ethanol reflux extraction process;

[0077] Retention rate of active ingredients: After enzymatic hydrolysis and ultrasonic-microwave synergistic extraction, the retention rates of *Millettia dielsiana* flavonoids, *Gynostemma pentaphyllum* lactone, and *Achyranthes bidentata* polysaccharide were 98.2%, 97.3%, and 96.7%, respectively; after microfiltration, the retention rates were 97.8%, 96.9%, and 96.4%, respectively, with a decrease of less than 0.5% in all cases.

[0078] Impurity removal and clarification effects: The removal rate of suspended particles reached 99.9%, and the transmittance of the clarified liquid reached 99.0%; the microbial removal rate reached 100%, with bacterial, mold, and yeast counts all <10 CFU / mL, and Escherichia coli not detected; the removal rate of macromolecular impurities reached 85.4%;

[0079] Synergistic biological effects: The clarified solution with a mass concentration of 1 mg / mL achieved a DPPH free radical scavenging rate of 84.6%, and a half-maximal inhibitory concentration (IC50) of 1 mg / mL. 50The concentration was 0.43 mg / mL; the clarified solution with a mass concentration of 0.5 mg / mL inhibited the NO release of LPS-induced RAW264.7 macrophages by 71.8%, and reduced the NO release to 35.7 μmol / L.

[0080] Process efficiency: Extraction time is 30 min, which is 93.75% shorter than the traditional process; solvent consumption is reduced by 60% and unit energy consumption is reduced by 45.2%.

[0081] Example 5

[0082] By weight, the formula of this embodiment is made from the following raw materials: 18 parts of Zhuang medicine Qianjinba, 13 parts of Yao medicine Lingxiangcao, 10 parts of Zhuang medicine Niudali, and 8 parts of Yao medicine Zoumatai; wherein, the molar ratio of Qianjinba flavonoids contained in Zhuang medicine Qianjinba to Lingxiangcao lactone contained in Yao medicine Lingxiangcao is precisely controlled to be 3:2.

[0083] The Zhuang medicine *Qianjinba* is the dried root of *Qianjinba*, a legume. Thin-layer chromatography analysis showed that its *Qianjinba* flavonoid content met the molar ratio requirements. The Yao medicine *Lingxiangcao* is the dried whole herb of *Lingxiangcao*, a plant in the Primulaceae family. High-performance liquid chromatography analysis showed that its *Lingxiangcao* lactone content met the aforementioned molar ratio. The Zhuang medicine *Niudali* is a dried tuberous root conforming to pharmacopoeia standards. On a dry basis, its *Niudali* polysaccharide content is 10.5%, ≥ the 10.0% limit specified in the claims, ensuring the basic content of the active ingredients in the raw materials.

[0084] This embodiment employs a preparation process combining enzymatic pretreatment with ultrasonic-microwave synergistic extraction. The specific steps are as follows:

[0085] The above-mentioned proportions of Zhuang medicinal herbs *Qianjinba*, Yao medicinal herbs *Lingxiangcao*, Zhuang medicinal herbs *Niudali*, and Yao medicinal herbs *Zoumatai* were each purified to remove impurities, mud, and non-medicinal parts. They were then dried in a 60℃ hot air drying oven until constant weight. After drying, each herb was separately fed into a universal pulverizer and pulverized, then passed through a 90-mesh standard sieve to obtain herb powder. The herb powders were then thoroughly mixed according to the formula ratio to obtain a mixed herb powder. This step of pulverizing the herbs to 90 mesh (within the 80-100 mesh range) increases the contact area between the herbs and the extraction solvent, improving subsequent extraction efficiency, while avoiding problems such as difficulty in filtration of the extract and excessive dissolution of impurities caused by excessive pulverization.

[0086] Take 1 kg of the above mixed medicinal powder, add 12 times its weight of deionized water, stir well, and adjust the pH of the system to 5.0. Then add cellulase and pectinase, where the cellulase activity is 5000 U / g and the amount added is 0.5% of the weight of the mixed medicinal powder, and the pectinase activity is 3000 U / g and the amount added is 0.3% of the weight of the mixed medicinal powder. Place the mixture in a 45℃ constant temperature water bath shaker and shake at 150 r / min for 2 hours for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, place the system in a 95℃ water bath for 10 minutes to inactivate the enzyme. After cooling to room temperature, the enzymatic hydrolysate is obtained. Cellulase can break down cellulose in the cell walls of medicinal materials, while pectinase can break down pectin. The synergistic effect of the two can effectively destroy the cell wall structure of medicinal materials, breaking the binding state between active ingredients and the cell wall, thus creating conditions for the dissolution of active ingredients in subsequent extraction steps. Compared with the process without enzymatic pretreatment, the dissolution rate of active ingredients can be increased by more than 20%. Enzyme inactivation treatment can avoid the continuous action of enzymes that would damage the structure of active ingredients, ensuring the stability of active ingredients.

[0087] The enzymatic hydrolysate was transferred to an ultrasonic-microwave synergistic extraction device. Extraction parameters were set as follows: ultrasonic power 220W, microwave power 320W, extraction temperature 60℃, and extraction time 30min. Stirring was continuous during extraction. After extraction, ultrasonic and microwave action was stopped. The system was allowed to cool to room temperature, and the residue was removed by filtration to obtain the initial extract. Ultrasonic-microwave synergistic extraction combines the cavitation effect of ultrasound with the thermal effect of microwaves. Ultrasonic cavitation creates tiny bubbles in the solvent that burst instantly, generating localized high pressure and accelerating the dissolution of active ingredients. Microwave thermal effect causes rapid vibration of solvent molecules, achieving uniform heating of the system and shortening the extraction time. In this embodiment, the ultrasonic power (200-250W) and microwave power (300-350W) ranged from 220W to 320W, ensuring extraction efficiency while avoiding problems such as rapid solvent evaporation and thermal degradation of active ingredients caused by excessively high power. Compared with traditional reflux extraction, the extraction time was shortened by 60%, and the retention rate of active ingredients was increased by 15%.

[0088] The initial extract was pressure filtered through a 0.22μm microporous membrane at a controlled pressure of 0.1MPa. After filtration, the filtrate was collected, yielding a clear liquid containing the active ingredients of the Zhuang and Yao medicinal herbs. Using a 0.22μm microporous membrane effectively removes tiny suspended particles, colloidal impurities, and microorganisms from the initial extract, resulting in a clear and transparent filtrate. Simultaneously, it does not retain small-molecule active ingredients from the Zhuang and Yao herbs, ensuring the content of these active ingredients. Pressure filtration further improves filtration efficiency, making it suitable for industrial production applications.

[0089] Results: Dissolution rate of active ingredients: The dissolution rate of flavonoids from *Millettia dielsiana* reached 92.6%, ginsenosides reached 92.3%, and polysaccharides from *Millettia dielsiana* reached 89.3%, which were improved by 24.4%, 21.8%, and 24.3% respectively compared with the traditional ethanol reflux extraction process;

[0090] Retention rate of active ingredients: After enzymatic hydrolysis and ultrasonic-microwave synergistic extraction, the retention rates of *Millettia dielsiana* flavonoids, *Gynostemma pentaphyllum* lactone, and *Achyranthes bidentata* polysaccharide were 98.4%, 97.7%, and 96.8%, respectively; after microfiltration, the retention rates were 98.0%, 97.3%, and 96.5%, respectively, with a decrease of less than 0.5% in all cases.

[0091] Impurity removal and clarification effects: The removal rate of suspended particles reached 99.9%, and the transmittance of the clarified liquid reached 99.2%; the microbial removal rate reached 100%, with bacterial, mold, and yeast counts all <10 CFU / mL, and Escherichia coli not detected; the removal rate of macromolecular impurities reached 85.7%;

[0092] Synergistic biological effects: The clarified solution with a mass concentration of 1 mg / mL achieved a DPPH free radical scavenging rate of 91.2% and a half-maximal inhibitory concentration (IC50) of 1 mg / mL. 50 The concentration was 0.31 mg / mL; the clarified solution with a mass concentration of 0.5 mg / mL inhibited the NO release of LPS-induced RAW264.7 macrophages by 79.3%, and reduced the NO release to 25.4 μmol / L.

[0093] Process efficiency: Extraction time is 30 min, which is 93.75% shorter than the traditional process; solvent consumption is reduced by 60% and unit energy consumption is reduced by 45.2%.

[0094] Example 6

[0095] By weight, the formula of this embodiment is made from the following raw materials: 18 parts of Zhuang medicine Qianjinba, 15 parts of Yao medicine Lingxiangcao, 10 parts of Zhuang medicine Niudali, and 8 parts of Yao medicine Zoumatai; wherein, the molar ratio of Qianjinba flavonoids contained in Zhuang medicine Qianjinba to Lingxiangcao lactone contained in Yao medicine Lingxiangcao is precisely controlled to be 3:2.

[0096] The Zhuang medicine *Qianjinba* is the dried root of *Qianjinba*, a legume. Thin-layer chromatography analysis showed that its *Qianjinba* flavonoid content met the molar ratio requirements. The Yao medicine *Lingxiangcao* is the dried whole herb of *Lingxiangcao*, a plant in the Primulaceae family. High-performance liquid chromatography analysis showed that its *Lingxiangcao* lactone content met the aforementioned molar ratio. The Zhuang medicine *Niudali* is a dried tuberous root conforming to pharmacopoeia standards. On a dry basis, its *Niudali* polysaccharide content is 10.5%, ≥ the 10.0% limit specified in the claims, ensuring the basic content of the active ingredients in the raw materials.

[0097] This embodiment employs a preparation process combining enzymatic pretreatment with ultrasonic-microwave synergistic extraction. The specific steps are as follows:

[0098] The above-mentioned proportions of Zhuang medicinal herbs *Qianjinba*, Yao medicinal herbs *Lingxiangcao*, Zhuang medicinal herbs *Niudali*, and Yao medicinal herbs *Zoumatai* were each purified to remove impurities, mud, and non-medicinal parts. They were then dried in a 60℃ hot air drying oven until constant weight. After drying, each herb was separately fed into a universal pulverizer and pulverized, then passed through a 90-mesh standard sieve to obtain herb powder. The herb powders were then thoroughly mixed according to the formula ratio to obtain a mixed herb powder. This step of pulverizing the herbs to 90 mesh (within the 80-100 mesh range) increases the contact area between the herbs and the extraction solvent, improving subsequent extraction efficiency, while avoiding problems such as difficulty in filtration of the extract and excessive dissolution of impurities caused by excessive pulverization.

[0099] Take 1 kg of the above mixed medicinal powder, add 12 times its weight of deionized water, stir well, and adjust the pH of the system to 5.0. Then add cellulase and pectinase, where the cellulase activity is 5000 U / g and the amount added is 0.5% of the weight of the mixed medicinal powder, and the pectinase activity is 3000 U / g and the amount added is 0.3% of the weight of the mixed medicinal powder. Place the mixture in a 45℃ constant temperature water bath shaker and shake at 150 r / min for 2 hours for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, place the system in a 95℃ water bath for 10 minutes to inactivate the enzyme. After cooling to room temperature, the enzymatic hydrolysate is obtained. Cellulase can break down cellulose in the cell walls of medicinal materials, while pectinase can break down pectin. The synergistic effect of the two can effectively destroy the cell wall structure of medicinal materials, breaking the binding state between active ingredients and the cell wall, thus creating conditions for the dissolution of active ingredients in subsequent extraction steps. Compared with the process without enzymatic pretreatment, the dissolution rate of active ingredients can be increased by more than 20%. Enzyme inactivation treatment can avoid the continuous action of enzymes that would damage the structure of active ingredients, ensuring the stability of active ingredients.

[0100] The enzymatic hydrolysate was transferred to an ultrasonic-microwave synergistic extraction device. Extraction parameters were set as follows: ultrasonic power 220W, microwave power 320W, extraction temperature 60℃, and extraction time 30min. Stirring was continuous during extraction. After extraction, ultrasonic and microwave action was stopped. The system was allowed to cool to room temperature, and the residue was removed by filtration to obtain the initial extract. Ultrasonic-microwave synergistic extraction combines the cavitation effect of ultrasound with the thermal effect of microwaves. Ultrasonic cavitation creates tiny bubbles in the solvent that burst instantly, generating localized high pressure and accelerating the dissolution of active ingredients. Microwave thermal effect causes rapid vibration of solvent molecules, achieving uniform heating of the system and shortening the extraction time. In this embodiment, the ultrasonic power (200-250W) and microwave power (300-350W) ranged from 220W to 320W, ensuring extraction efficiency while avoiding problems such as rapid solvent evaporation and thermal degradation of active ingredients caused by excessively high power. Compared with traditional reflux extraction, the extraction time was shortened by 60%, and the retention rate of active ingredients was increased by 15%.

[0101] The initial extract was pressure filtered through a 0.22μm microporous membrane at a controlled pressure of 0.1MPa. After filtration, the filtrate was collected, yielding a clear liquid containing the active ingredients of the Zhuang and Yao medicinal herbs. Using a 0.22μm microporous membrane effectively removes tiny suspended particles, colloidal impurities, and microorganisms from the initial extract, resulting in a clear and transparent filtrate. Simultaneously, it does not retain small-molecule active ingredients from the Zhuang and Yao herbs, ensuring the content of these active ingredients. Pressure filtration further improves filtration efficiency, making it suitable for industrial production applications.

[0102] Results: Dissolution rate of active ingredients: The dissolution rate of flavonoids from *Millettia dielsiana* reached 92.8%, ginsenosides reached 93.5%, and polysaccharides from *Millettia dielsiana* reached 89.6%, which were improved by 24.6%, 23.0%, and 24.6% respectively compared with the traditional ethanol reflux extraction process;

[0103] Retention rate of active ingredients: After enzymatic hydrolysis and ultrasonic-microwave synergistic extraction, the retention rates of *Millettia dielsiana* flavonoids, *Gynostemma pentaphyllum* lactone, and *Achyranthes bidentata* polysaccharide were 98.5%, 97.9%, and 96.9%, respectively; after microfiltration, the retention rates were 98.1%, 97.5%, and 96.6%, respectively, with a decrease of less than 0.5%.

[0104] Impurity removal and clarification effects: The removal rate of suspended particles reached 99.9%, and the transmittance of the clarified liquid reached 99.4%; the microbial removal rate reached 100%, with bacterial, mold, and yeast counts all <10 CFU / mL, and Escherichia coli not detected; the removal rate of macromolecular impurities reached 85.9%;

[0105] Synergistic biological effects: The clarified solution with a mass concentration of 1 mg / mL achieved a DPPH free radical scavenging rate of 94.8% and a half-maximal inhibitory concentration (IC50) of 1 mg / mL. 50 The concentration was 0.26 mg / mL; the clarified solution with a mass concentration of 0.5 mg / mL inhibited the NO release of LPS-induced RAW264.7 macrophages by 84.5%, and reduced the NO release to 20.0 μmol / L.

[0106] Process efficiency: Extraction time is 30 min, which is 93.75% shorter than the traditional process; solvent consumption is reduced by 60% and unit energy consumption is reduced by 45.2%.

[0107] Comparative Example

[0108] By weight, the formula of this embodiment is made from the following raw materials: 18 parts of Zhuang medicine Qianjinba, 12 parts of Yao medicine Lingxiangcao, 10 parts of Zhuang medicine Niudali, and 8 parts of Yao medicine Zoumatai; wherein, the molar ratio of Qianjinba flavonoids contained in Zhuang medicine Qianjinba to Lingxiangcao lactone contained in Yao medicine Lingxiangcao is precisely controlled to be 3:2.

[0109] The Zhuang medicine *Qianjinba* is the dried root of *Qianjinba*, a legume. Thin-layer chromatography analysis showed that its *Qianjinba* flavonoid content met the molar ratio requirements. The Yao medicine *Lingxiangcao* is the dried whole herb of *Lingxiangcao*, a plant in the Primulaceae family. High-performance liquid chromatography analysis showed that its *Lingxiangcao* lactone content met the aforementioned molar ratio. The Zhuang medicine *Niudali* is a dried tuberous root conforming to pharmacopoeia standards. On a dry basis, its *Niudali* polysaccharide content is 10.5%, ≥ the 10.0% limit specified in the claims, ensuring the basic content of the active ingredients in the raw materials.

[0110] This embodiment employs a preparation process combining enzymatic pretreatment with ultrasonic-microwave synergistic extraction. The specific steps are as follows:

[0111] The above-mentioned proportions of Zhuang medicinal herbs *Qianjinba*, Yao medicinal herbs *Lingxiangcao*, Zhuang medicinal herbs *Niudali*, and Yao medicinal herbs *Zoumatai* were each purified to remove impurities, mud, and non-medicinal parts. They were then dried in a 60℃ hot air drying oven until constant weight. After drying, each herb was separately fed into a universal pulverizer and pulverized, then passed through a 90-mesh standard sieve to obtain herb powder. The herb powders were then thoroughly mixed according to the formula ratio to obtain a mixed herb powder. This step of pulverizing the herbs to 90 mesh (within the 80-100 mesh range) increases the contact area between the herbs and the extraction solvent, improving subsequent extraction efficiency, while avoiding problems such as difficulty in filtration of the extract and excessive dissolution of impurities caused by excessive pulverization.

[0112] Take 1 kg of the above mixed medicinal powder, add 12 times its weight of deionized water, stir well, and adjust the pH of the system to 5.0. Then add cellulase and pectinase, where the cellulase activity is 5000 U / g and the amount added is 0.5% of the weight of the mixed medicinal powder, and the pectinase activity is 3000 U / g and the amount added is 0.3% of the weight of the mixed medicinal powder. Place the mixture in a 45℃ constant temperature water bath shaker and shake at 150 r / min for 2 hours for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, place the system in a 95℃ water bath for 10 minutes to inactivate the enzyme. After cooling to room temperature, the enzymatic hydrolysate is obtained. Cellulase can break down cellulose in the cell walls of medicinal materials, while pectinase can break down pectin. The synergistic effect of the two can effectively destroy the cell wall structure of medicinal materials, breaking the binding state between active ingredients and the cell wall, thus creating conditions for the dissolution of active ingredients in subsequent extraction steps. Compared with the process without enzymatic pretreatment, the dissolution rate of active ingredients can be increased by more than 20%. Enzyme inactivation treatment can avoid the continuous action of enzymes that would damage the structure of active ingredients, ensuring the stability of active ingredients.

[0113] The enzymatic hydrolysate was transferred to an ultrasonic-microwave synergistic extraction device. Extraction parameters were set as follows: ultrasonic power 220W, microwave power 320W, extraction temperature 60℃, and extraction time 30min. Stirring was continuous during extraction. After extraction, ultrasonic and microwave action was stopped. The system was allowed to cool to room temperature, and the residue was removed by filtration to obtain the initial extract. Ultrasonic-microwave synergistic extraction combines the cavitation effect of ultrasound with the thermal effect of microwaves. Ultrasonic cavitation creates tiny bubbles in the solvent that burst instantly, generating localized high pressure and accelerating the dissolution of active ingredients. Microwave thermal effect causes rapid vibration of solvent molecules, achieving uniform heating of the system and shortening the extraction time. In this embodiment, the ultrasonic power (200-250W) and microwave power (300-350W) ranged from 220W to 320W, ensuring extraction efficiency while avoiding problems such as rapid solvent evaporation and thermal degradation of active ingredients caused by excessively high power. Compared with traditional reflux extraction, the extraction time was shortened by 60%, and the retention rate of active ingredients was increased by 15%.

[0114] The initial extract was pressure filtered through a 0.22μm microporous membrane at a controlled pressure of 0.1MPa. After filtration, the filtrate was collected, yielding a clear liquid containing the active ingredients of the Zhuang and Yao medicinal herbs. Using a 0.22μm microporous membrane effectively removes tiny suspended particles, colloidal impurities, and microorganisms from the initial extract, resulting in a clear and transparent filtrate. Simultaneously, it does not retain small-molecule active ingredients from the Zhuang and Yao herbs, ensuring the content of these active ingredients. Pressure filtration further improves filtration efficiency, making it suitable for industrial production applications.

[0115] The specific content is shown in the table below:

[0116] Zhuang medicine Qianjinba Yao medicine and fragrant herbs Example 1 15 12 Example 2 17 12 Example 3 20 12 Example 4 18 10 Example 5 18 13 Example 6 18 15 Comparative Example 18 12

[0117] As shown in the table above, Examples 1 to 3, by changing the weight of the Zhuang herb *Smilax glabra*, while keeping other raw material ratios, preparation process parameters, and the 3:2 molar ratio of *Smilax glabra* flavonoids to *Gynostemma pentaphyllum* constant, systematically verified the influence of *Smilax glabra* content on the product efficacy. The results showed that as the *Smilax glabra* content gradually increased within the range of 15-20 parts, the core effects of the compound Zhuang-Yao medicinal active ingredients exhibited a significant positive correlation: the dissolution rate of *Smilax glabra* flavonoids increased from 90.1% to 94.2%, with the improvement compared to the traditional process increasing from 21.9% to 26.0%; in terms of synergistic biological effects, the DPPH free radical scavenging rate increased from 85.3% to 93.5%, and the half-maximal concentration (IC50) increased. 50It decreased from 0.41 mg / mL to 0.28 mg / mL, the NO release inhibition rate increased from 72.4% to 82.7%, and the NO release amount decreased from 34.8 μmol / L to 22.6 μmol / L, fully demonstrating that as one of the core active ingredients, the synergy between Flemingia philippinensis flavonoids and components such as Lysimachia foenum-graecum Hance lactone and Millettia speciosa Champ polysaccharide is concentration-dependent. At the same time, the retention rate of active ingredients, the impurity removal effect and the process efficiency of the three examples all remained at a stable and excellent level. The removal rate of suspended particles reached 99.9%, the removal rate of microorganisms was 100%, the extraction time was shortened by more than 93.75% compared with the traditional process, and the solvent consumption was reduced by 60%, verifying the stability and reliability of the formula and preparation process. Among them, when there are 15 parts of Flemingia philippinensis, the product can meet the basic application requirements. When there are 17-18 parts, the effect is balanced and the raw material cost is controllable. When there are 20 parts, the synergistic biological effect is optimal, providing sufficient experimental basis for the formula selection under different application scenarios, and also verifying the scientificity and rationality of the weight range limit of 15-20 parts of Flemingia philippinensis in claim 1.

[0118] Examples 4 to 6 adjusted the weight parts of Lysimachia foenum-graecum Hance, a Yao medicine, and systematically explored the influence law of the content of Lysimachia foenum-graecum Hance on the effects of active ingredients of the compound Zhuang and Yao medicine under the condition of keeping the ratio of other raw materials, the preparation process parameters and the molar ratio of Flemingia philippinensis flavonoids to Lysimachia foenum-graecum Hance lactone unchanged at 3:2. The results showed that as the content of Lysimachia foenum-graecum Hance in the range of 10-15 parts gradually increased, the core effect of the product showed a significant positive optimization trend: the dissolution rate of Lysimachia foenum-graecum Hance lactone increased from 90.2% to 93.5%, and the improvement amplitude compared with the traditional process increased from 19.7% to 23.0%; the synergistic biological effect was particularly prominent, the DPPH free radical scavenging rate increased from 84.6% to 94.8%, and the half-maximal inhibitory concentration IC 50Reducing the concentration of ginsenoside from 0.43 mg / mL to 0.26 mg / mL increased the inhibition rate of LPS-induced NO release from RAW264.7 macrophages from 71.8% to 84.5%, and decreased the NO release from 35.7 μmol / L to 20.0 μmol / L. This fully demonstrates that ginsenoside, as a key synergistic component, significantly enhances the synergistic effect with ginseng flavonoids and ginseng polysaccharide when its content is increased. Meanwhile, the retention rate of active ingredients, impurity removal effect, and process efficiency of the three examples remained stable and excellent. The removal rate of suspended particles reached 99.9%, the microbial removal rate was 100%, the extraction time was shortened by 93.75% compared to the traditional process, and the solvent consumption was reduced by 60%, verifying the stability and universality of the formulation and preparation process. Among them, 10 parts of the product with *Lingxiangcao* can meet the efficacy requirements of basic application scenarios, 13 parts achieve the optimal balance between efficacy and raw material cost, and 15 parts achieve the peak of synergistic biological effects. This provides a precise formula basis for the development of products with different efficacy requirements and cost budgets, and further confirms the scientificity and rationality of the weight range of 10-15 parts of *Lingxiangcao* in claim 1, providing multiple options for the industrial application of compound Zhuang and Yao medicines.

[0119] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A compound formula of active ingredients from Zhuang and Yao medicines, characterized in that, Made from the following raw materials in parts by weight: The ingredients are 15-20 parts of the Zhuang medicine *Qianjinba*, 10-15 parts of the Yao medicine *Lingxiangcao*, 8-12 parts of the Zhuang medicine *Niudali*, and 6-10 parts of the Yao medicine *Zoumatai*; and the molar ratio of *Qianjinba* flavonoids contained in the Zhuang medicine *Qianjinba* to *Lingxiangcao* lactones contained in the Yao medicine *Lingxiangcao* is 3:

2.

2. The compound Zhuang-Yao medicine active ingredient synergistic formulation according to claim 1, characterized in that, The medicinal herb *Qianjinba* is the dried root of *Qianjinba*, a legume.

3. The compound Zhuang-Yao medicine active ingredient synergistic formulation according to claim 1, characterized in that, The Yao medicine Lingxiangcao is the dried whole herb of Lingxiangcao, a plant in the Primulaceae family.

4. The compound Zhuang-Yao medicine active ingredient synergistic formulation according to claim 1, characterized in that, The content of *Niu Dali* polysaccharide in the Zhuang medicine is ≥10.0%, calculated on a dry basis.

5. The compound Zhuang-Yao medicine active ingredient synergistic formulation according to claim 1, characterized in that, The medicinal materials must be pulverized to 80-100 mesh before being mixed.

6. A method for preparing a compound Zhuang-Yao medicine active ingredient synergistic formulation, used to prepare the compound Zhuang-Yao medicine active ingredient synergistic formulation according to any one of claims 1-5, characterized in that, Includes the following steps: S1. Pre-treat each medicinal material in the formula to obtain medicinal powder; S2. Add cellulase and pectinase to the medicinal powder for enzymatic pretreatment to obtain enzymatic hydrolysate; S3. Extract the enzymatic hydrolysate using a combination of ultrasound and microwave to obtain the initial extract.

7. The compound Zhuang-Yao medicine active ingredient synergistic formulation according to claim 6, characterized in that, In step S2, the cellulase activity is ≥5000 U / g, and the pectinase activity is ≥3000 U / g.

8. The compound Zhuang-Yao medicine active ingredient synergistic formulation according to claim 6, characterized in that, In step S2, the mass of deionized water added to the medicinal powder is 10-15 times the mass of the medicinal powder.

9. The compound Zhuang-Yao medicine active ingredient synergistic formulation according to claim 6, characterized in that, In step S3, the ultrasonic power is 200-250W and the microwave power is 300-350W.

10. The compound Zhuang-Yao medicine active ingredient synergistic formulation according to claim 6, characterized in that, The process also includes step S4, in which the initial extract is filtered through a 0.22μm microporous membrane to obtain a clarified solution of the compound Zhuang-Yao medicinal active ingredients.

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