A constant temperature cell culture device

Abstract

This invention relates to the field of cell culture technology and provides a constant-temperature cell culture device, including a shell, a door, a slide, a support, and a turntable. The door is installed at the opening of the shell, the slide is installed inside the shell, the support is installed on the slide, and the turntable is installed in the middle of the support. It also includes a lifting assembly, mounted on the support, for lifting and lowering culture dishes inside the turntable. The beneficial effects of this invention are: by designing the sliding grooves of multiple hollow toothed rings with reverse angles, the hollow toothed ring near the door can drive its corresponding loading rack upwards during the movement of the slide, allowing operators to retrieve culture dishes without reaching inside the shell. By sealing other culture dishes that do not require operation inside the shell, the impact of external contamination and temperature on the internal cell culture is reduced, enabling operators to handle individual culture dishes even when multiple culture dishes are being cultured simultaneously.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, and in particular relates to a constant temperature cell culture device. Background Technology

[0002] Isothermal cell culture is a core foundational technology in fields such as biomedical research, drug development, and cell engineering. Its core requirements are to provide a precise isothermal environment, a sterile operating space, a convenient way to handle culture dishes, and conditions to ensure uniform cell growth in the culture medium and avoid cell deposition and adhesion.

[0003] Most existing isothermal cell culture equipment uses fixed or mobile culture dish carriers. Retrieving and placing these dishes requires fully opening the equipment door, and staff must insert their hands or tools into the chamber. This allows microorganisms and dust from the outside air to easily enter the chamber and contaminate other culture dishes that have not yet been retrieved. During the opening of the door and movement of the carrier, the isothermal air inside the chamber exchanges rapidly with the ambient air outside, causing short-term temperature fluctuations. Since most human cells are sensitive to temperature, these fluctuations can easily lead to decreased cell activity or even apoptosis.

[0004] Existing equipment only allows surface cells in the culture dish to effectively absorb nutrients from the culture medium. Substrate cells grow slowly due to nutrient deficiency, affecting the progress of experiments. Furthermore, long-term deposition can cause cells to adhere tightly to the culture dish wall, making them difficult to digest and passage. Adhesion may also lead to abnormal cell differentiation, resulting in inefficient cell culture. Summary of the Invention

[0005] The purpose of this invention is to provide a constant temperature cell culture device, which aims to solve the technical problems existing in the prior art mentioned in the background.

[0006] This invention is implemented as follows: a constant-temperature cell culture device includes a shell, a door, a carriage, a support column, and a turntable. The door is installed at the opening of the shell, the carriage is installed inside the shell, the support column is installed on the carriage, and the turntable is installed in the middle of the support column. The device also includes: A lifting assembly, mounted on a support column, is used to lift and lower the culture dish inside the turntable. The lifting assembly includes an annular plate fixedly connected to the support column. Multiple first supports are mounted on the wall of the annular plate. Hollow toothed rings are rotatably connected to the bottom of each first support. Second supports are fixedly connected to the wall of the annular plate. A toothed ring plate is slidably connected inside each of the multiple first supports. A first transmission gear is rotatably connected to the bottom of the second support, meshing with the inner wall of the toothed ring plate. A rack is fixedly connected to the bottom of the inner cavity of the outer shell. A third transmission gear is rotatably connected inside the support column, meshing with the rack. A second transmission gear is connected between the first and third transmission gears. A clamping assembly, mounted on a turntable, is used to limit and clamp the culture dish. A cell oscillation component, located inside the outer shell, is used to prevent cell deposition in the culture dish; The air curtain assembly is located inside the housing to prevent external environmental contamination of the interior.

[0007] As a preferred technical solution of the present invention: the interior of each hollow toothed ring is provided with a sliding groove adapted to the convex toothed ring plate, and the sliding groove direction of one hollow toothed ring near the opening of the outer shell is opposite to that of the other hollow toothed rings.

[0008] As another preferred technical solution of the present invention: a plurality of loading racks are slidably connected inside the turntable, a first spring is fixedly connected between the bottom of the loading rack and the lower surface of the turntable, and a first baffle is slidably connected to the side of the loading rack near the culture dish.

[0009] As another preferred technical solution of the present invention: the clamping assembly includes a support plate fixedly connected to the bottom of the loading frame, a gear shaft rotatably connected to the top of the support plate, a second spring fixedly connected between the gear shaft and the support plate, clamping plates symmetrically slidably connected to the bottom of the loading frame, both clamping plates meshing with the gear shaft, a gear plate slidably connected inside the loading frame and below the clamping plates, a third spring fixedly connected between the gear plate and the inner wall of the loading frame, and a stop rod fixedly connected to the bottom of the turntable, the bottom of the stop rod being inclined and fitting against the side of the gear plate away from the gear shaft.

[0010] As another preferred technical solution of the present invention: the cell oscillation assembly includes an electrically driven shaft installed at the bottom of the inner cavity of the outer shell, a second shaft rotatably connected to the bottom of the inner cavity of the outer shell, a transmission belt connecting the second shaft and the electrically driven shaft, an ultrasonic generator installed at the top of the second shaft, arc-shaped baffles symmetrically fixedly connected to the side wall of the ultrasonic generator, a sleeve is fitted at the bottom of the inner cavity of the outer shell and outside the second shaft, a slide cylinder is slidably connected inside the sleeve, cylinders are symmetrically fixedly connected to the inner wall of the slide cylinder, a fourth spring is fixedly connected between the bottom of the slide cylinder and the bottom of the inner cavity of the outer shell, a cover is rotatably connected to the top of the slide cylinder, the cover is slidably fitted on the ultrasonic generator, and the cylinder is attached to the surface of the arc-shaped baffle.

[0011] As another preferred technical solution of the present invention: the ultrasonic generator generates ultrasonic waves vertically upwards, and when the cylinder is not squeezed by the arc-shaped baffle, the fourth spring extends and pushes the cover to contact the bottom of the loading frame.

[0012] As another preferred technical solution of the present invention: the air curtain assembly includes an air outlet chamber fixedly connected to the top of the inner cavity of the outer shell, an air chamber communicating with the inside of the air outlet chamber fixedly connected to the top of the inner cavity of the outer shell, a sliding plate slidably connected inside the air chamber, a fifth spring fixedly connected between the sliding plate and the inner wall of the air chamber, a round rod slidably connected to the slide, a sixth spring fixedly connected between the end of the round rod and the slide, and the bottom opening of the air outlet chamber facing the opening of the outer shell at a 45-degree angle.

[0013] As another preferred technical solution of the present invention: the side wall of the turntable is rotatably connected to a square plate, the square plate is slidably connected to the inner cavity of the outer shell, the bottom of the square plate is fixedly connected to a partition curtain, the side of the partition curtain away from the square plate is fixedly connected to the inner wall of the outer shell, and the lower half of the inner cavity of the outer shell is formed into a closed space by the turntable, the square plate and the partition curtain.

[0014] As another preferred technical solution of the present invention: the lower surface of the first baffle and the upper surface of the turntable are provided with magnetic plates with opposite magnetic properties.

[0015] The beneficial effects of the embodiments of the present invention are as follows: 1. By using the reverse angle design of the sliding grooves of multiple hollow toothed rings, the hollow toothed rings near the hatch can drive the corresponding loading rack to rise upwards during the movement of the slide, so that the staff can pick up the culture dishes without reaching into the shell, improving the convenience of operation. By sinking and sealing other culture dishes that do not need to be operated inside the shell, the impact of external contamination and temperature on the internal cell culture is reduced, allowing the staff to pick up and put down a single culture dish when multiple culture dishes are being cultured at the same time.

[0016] 2. The ultrasonic generator at the top of the second rotating shaft releases ultrasonic waves vertically upwards, causing the cells in the culture dish to float upwards in the culture medium due to the ultrasonic waves, thus preventing the cells from settling at the bottom of the culture dish. The rotation of the ultrasonic generator driven by the second rotating shaft allows the ultrasonic waves to act on the cells in the culture dish at different angles, reducing cell adhesion to the culture dish wall. When the slide moves downwards, the fourth spring elastically contracts and stores force. After the arc-shaped baffle rotates to disengage from the cylinder, the fourth spring rebounds and causes the slide and the cover to vibrate against the bottom of the loading rack. The combination of vibration and ultrasonic waves keeps the cells in the culture dish in a suspended state during the culture process, improving culture efficiency. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure provided in an embodiment of the present invention; Figure 2 This is a three-dimensional schematic diagram of the overall structure provided in an embodiment of the present invention; Figure 3 This is a partial schematic diagram of the internal structure provided in an embodiment of the present invention; Figure 4 This is a partial schematic diagram of the lifting component structure provided in an embodiment of the present invention; Figure 5 This is an exploded view of the lifting assembly structure provided in an embodiment of the present invention; Figure 6 This is a partial cross-sectional view of the lifting component structure provided in an embodiment of the present invention; Figure 7 This is a cross-sectional view of the internal structure provided in an embodiment of the present invention; Figure 8 This is a schematic diagram of the clamping assembly structure provided in an embodiment of the present invention; Figure 9 This is an exploded view of the clamping assembly structure provided in an embodiment of the present invention; Figure 10 This is a schematic diagram of the internal structure provided in an embodiment of the present invention; Figure 11 This is a schematic diagram of the cell oscillation component structure explosion provided in an embodiment of the present invention; Figure 12 This is a schematic internal cross-sectional view of the cell oscillation component structure provided in an embodiment of the present invention; Figure 13 This is a schematic internal cross-sectional view of the air curtain assembly structure provided in an embodiment of the present invention.

[0018] In the diagram: 1. Outer shell; 2. Door; 3. Skid; 4. Support column; 5. Turntable; 6. Lifting assembly; 7. Clamping assembly; 8. Cellular oscillation assembly; 9. Air curtain assembly; 501. Square panel; 502. Dividing screen; 61. Annular plate; 62. First support; 63. Hollow toothed ring; 64. Second support; 65. First transmission rack; 66. Convex toothed ring plate; 67. Second transmission rack; 68. Third transmission rack; 69. Rack; 610. Loading frame; 611. First baffle; 612. First spring; 71. Support plate; 72. Gear shaft; 73. Second spring; 74. Clamping plate; 75. Gear plate; 76. Third spring; 77. Support rod; 81. Electric drive shaft; 82. Transmission belt; 83. Housing; 84. Slide cylinder; 85. Fourth spring; 86. Second shaft; 87. Ultrasonic generator; 88. Arc-shaped baffle; 89. Cylinder; 810. Cover; 91. Air outlet; 92. Air chamber; 93. Slide plate; 94. Fifth spring; 95. Round rod; 96. Sixth spring. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0020] It is understood that the terms “first,” “second,” etc., used in this application may be used herein to describe various elements, but unless otherwise stated, these elements are not limited by these terms. These terms are used only to distinguish one element from another.

[0021] like Figures 1 to 7 As shown, in one embodiment, a constant-temperature cell culture device is proposed, including a shell 1, a door 2, a carriage 3, a support 4, and a turntable 5. The door 2 is installed at the opening of the shell 1, the carriage 3 is installed inside the shell 1, the support 4 is installed on the carriage 3, and the turntable 5 is installed in the middle of the support 4. The device also includes: The lifting assembly 6, mounted on the support column 4, is used to lift and lower the culture dish inside the turntable 5. The lifting assembly 6 includes an annular plate 61 fixedly connected to the support column 4. Multiple first supports 62 are installed on the wall of the annular plate 61. Hollow toothed rings 63 are rotatably connected to the bottom of each first support 62. Second supports 64 are fixedly connected to the wall of the annular plate 61. A toothed ring plate 66 is slidably connected inside each of the multiple first supports 62. A first transmission gear 65 that meshes with the inner wall of the toothed ring plate 66 is rotatably connected to the bottom of the second support 64. A rack 69 is fixedly connected to the bottom of the inner cavity of the outer shell 1. A third transmission gear 68 that meshes with the rack 69 is rotatably connected inside the support column 4. A second transmission gear 67 is connected between the first transmission gear 65 and the third transmission gear 68. Clamping component 7, mounted on turntable 5, is used to limit and clamp the culture dish; The cell oscillation component 8 is located inside the outer shell 1 to prevent cell deposition in the culture dish; The air curtain assembly 9 is located inside the housing 1 to prevent external environmental pollution from affecting the interior of the housing 1.

[0022] In practical application, when an operator needs to pick up or place a culture dish on the turntable 5, the turntable 5 is rotated on the surface of the support column 4, moving the culture dish to be picked up or placed closer to the door 2. After the turntable 5 rotates the culture dish to the designated position, the operator manipulates the slide 3 to move closer to the door 2. When the slide 3 moves, it will drive the support column 4 and the third transmission rack 68 inside the support column 4 to move together. During the movement, the third transmission rack 68 will mesh with and rotate with the rack 69. When the third transmission rack 68 rotates, it will interact with the second transmission rack 67. The meshing of the second transmission rack 67 and the first transmission rack 65 causes the toothed ring plate 66 meshing with the first transmission rack 65 to rotate circumferentially below the first support 62. During the rotation, the toothed ring plate 66 slides inside multiple hollow toothed rings 63, causing multiple hollow toothed rings 63 to rotate simultaneously. At this time, the hollow toothed ring 63 near the hatch 2 rotates clockwise under the sliding action of the toothed ring plate 66. During the rotation, the meshing drives the loading rack 610 near the hatch 2 to move upward, so that the loading rack 610, carrying the loaded culture dish, rises together to the level of the turntable 5.

[0023] At this time, because the internal grooves of the other hollow toothed rings 63 are oriented in opposite directions, during the rotation of the toothed ring plate 66, the other hollow toothed rings 63 will rotate counterclockwise, thereby causing the other loading racks 610, except for the one near the hatch 2, to sink together. When the loading racks 610 sink to the point where the first baffle 611 contacts the upper surface of the turntable 5, the magnetic plate on the first baffle 611 will attract the magnetic plate on the turntable 5, so that the multiple culture dishes that do not need to be picked up or put down can be protected from external contamination under the enclosure of the first baffle 611 and the turntable 5.

[0024] When the loading rack 610 moves downward, the first spring 612 extends elastically; when the loading rack 610 moves upward, the first spring 612 stretches elastically. The tension of the first spring 612 makes the loading rack 610 and the hollow toothed ring 63 mesh tightly, so that the loading rack 610 will not shake during user operation.

[0025] like Figure 5 As shown, in a preferred embodiment of the present invention, the hollow toothed ring 63 is provided with a sliding groove that matches the convex toothed ring plate 66. Among the multiple hollow toothed rings 63, the sliding groove of the hollow toothed ring 63 closest to the opening of the outer shell 1 is opposite to that of the other hollow toothed rings 63.

[0026] In practical applications, the embodiments of the present invention utilize a reverse-angle design of the sliding grooves of multiple hollow toothed rings 63. This allows the hollow toothed ring 63 near the door 2 to lift its corresponding loading rack 610 upwards during the movement of the slide 3. This enables staff to retrieve the culture dishes without reaching into the outer shell 1, improving operational convenience. By sealing other culture dishes that do not require operation inside the outer shell 1, the impact of external contamination and temperature on internal cell culture is reduced. This allows staff to retrieve and place individual culture dishes even when multiple culture dishes are being cultured simultaneously.

[0027] like Figure 7 and Figure 8 As shown, in another preferred embodiment of the present invention, a plurality of loading racks 610 are slidably connected inside the turntable 5, a first spring 612 is fixedly connected between the bottom of the loading rack 610 and the lower surface of the turntable 5, and a first baffle 611 is slidably connected to the side of the loading rack 610 near the culture dish.

[0028] like Figures 7 to 9 As shown, in another preferred embodiment of the present invention, the clamping assembly 7 includes a support plate 71 fixedly connected to the bottom of the loading frame 610, a gear shaft 72 rotatably connected to the top of the support plate 71, a second spring 73 fixedly connected between the gear shaft 72 and the support plate 71, clamping plates 74 symmetrically slidably connected to the bottom of the loading frame 610, both clamping plates 74 meshing with the gear shaft 72, a gear plate 75 slidably connected inside the loading frame 610 and below the clamping plates 74, a third spring 76 fixedly connected between the gear plate 75 and the inner wall of the loading frame 610, and a stop rod 77 fixedly connected to the bottom of the turntable 5, the bottom of the stop rod 77 being inclined and fitting against the side of the gear plate 75 away from the gear shaft 72.

[0029] In practical application of this invention, when it is necessary to place a culture dish on the loading rack 610, the operator inserts the culture dish between the clamps 74. As the distance between the clamps 74 increases, it will drive the gear shaft 72 to rotate. At this time, the second spring 73 at the bottom of the gear shaft 72 twists and generates torque, so that the gear shaft 72 has a reverse rotation torque, thereby clamping and fixing the culture dish by the clamps 74 that mesh with the gear shaft 72.

[0030] When the petri dish extends out of the outer shell 1 and needs to be retrieved by the staff, the loading rack 610 slides upward inside the turntable 5, which causes the inclined surface of the bottom abutment 77 of the turntable 5 to press against the toothed plate 75, causing the toothed plate 75 to slide closer to the toothed shaft 72 and engage with the toothed shaft 72. After the toothed shaft 72 engages with the toothed plate 75, it will rotate. The rotation of the toothed shaft 72 will cause the two clamping plates 74 to engage and slide relatively far apart. After the distance between the clamping plates 74 increases, they no longer clamp the petri dish, so that the staff can easily remove the petri dish from between the clamping plates 74.

[0031] like Figures 10 to 12 As shown, in another preferred embodiment of the present invention, the cell oscillation assembly 8 includes an electrically driven shaft 81 installed at the bottom of the inner cavity of the outer shell 1, a second shaft 86 rotatably connected to the bottom of the inner cavity of the outer shell 1, a transmission belt 82 drivingly connecting the second shaft 86 and the electrically driven shaft 81, an ultrasonic generator 87 installed on the top of the second shaft 86, an arc-shaped baffle 88 symmetrically fixedly connected to the side wall of the ultrasonic generator 87, a housing 83 sleeved at the bottom of the inner cavity of the outer shell 1 and outside the second shaft 86, a sliding cylinder 84 slidably connected inside the housing 83, a cylinder 89 symmetrically fixedly connected to the inner wall of the sliding cylinder 84, a fourth spring 85 fixedly connected between the bottom of the sliding cylinder 84 and the bottom of the inner cavity of the outer shell 1, a cover 810 rotatably connected to the top of the sliding cylinder 84, the cover 810 slidably fitting on the ultrasonic generator 87, and the cylinder 89 abutting the surface of the arc-shaped baffle 88.

[0032] In practical application, when the electrically driven shaft 81 rotates, it drives the second shaft 86 to rotate together via the transmission belt 82. At this time, the ultrasonic generator 87 at the top of the second shaft 86 releases ultrasonic waves vertically upwards, causing the cells in the culture dish to float upwards in the culture medium under the action of ultrasonic waves, thus preventing the cells from settling at the bottom of the culture dish. By driving the ultrasonic generator 87 to rotate via the second shaft 86, ultrasonic waves can act on the cells in the culture dish at different angles, reducing the adhesion of cells to the wall of the culture dish.

[0033] During the rotation of the second rotating shaft 86, the arc-shaped baffle 88 on the surface will rotate together. When the arc-shaped baffle 88 rotates, it will cause the slide cylinder 84 to move downward inside the casing 83 by squeezing the cylinder 89. After the slide cylinder 84 moves downward, the fourth spring 85 elastically contracts and stores force. When the arc-shaped baffle 88 rotates to the point of disengaging from the cylinder 89, the fourth spring 85 rebounds and causes the slide cylinder 84 and the cover 810 to vibrate the bottom of the loading frame 610. By combining vibration and ultrasound, the cells in the culture dish can be kept in a suspended state during the culture process, thereby improving the culture efficiency.

[0034] like Figure 12 As shown, in another preferred embodiment of the present invention, the ultrasonic generator 87 generates ultrasonic waves vertically upwards, and when the cylinder 89 is not squeezed by the arc-shaped baffle 88, the fourth spring 85 extends and pushes the cover 810 to abut against the bottom of the loading frame 610.

[0035] like Figure 13As shown, in another preferred embodiment of the present invention, the air curtain assembly 9 includes an air outlet chamber 91 fixedly connected to the top of the inner cavity of the outer shell 1, an air chamber 92 connected to the inside of the air outlet chamber 91 fixedly connected to the top of the inner cavity of the outer shell 1, a sliding plate 93 slidably connected inside the air chamber 92, a fifth spring 94 fixedly connected between the sliding plate 93 and the inner wall of the air chamber 92, a round rod 95 slidably connected to the slide 3, a sixth spring 96 fixedly connected between the end of the round rod 95 and the slide 3, and the bottom opening of the air outlet chamber 91 faces the opening of the outer shell 1 at a 45-degree angle.

[0036] In practical application, when the slide 3 moves towards the side closer to the hatch 2, the slide 3 will move together with the round rod 95 via the sixth spring 96. When the end of the round rod 95 moves to fit against the slide plate 93, the slide 3 continues to move, causing the round rod 95 to squeeze the slide plate 93 into the air chamber 92, and causing the fifth spring 94 to be compressed and elastically contracted. After the slide plate 93 slides inside the air chamber 92, the air inside the air chamber 92 will transfer to the air outlet chamber 91 and be discharged outward through the opening of the air outlet chamber 91. At this time, because the opening of the air outlet chamber 91 is tilted outward at a 45-degree angle, the air curtain formed by the air blown out from the air outlet chamber 91 can form a barrier between the outside air and the inside of the outer shell 1, reducing the impact of the external environment on the inside of the outer shell 1.

[0037] like Figure 7 As shown, in another preferred embodiment of the present invention, a square plate 501 is rotatably connected to the side wall of the turntable 5. The square plate 501 is slidably connected to the inner cavity of the outer shell 1. A partition curtain 502 is fixedly connected to the bottom of the square plate 501. The side of the partition curtain 502 away from the square plate 501 is fixedly connected to the inner wall of the outer shell 1. The lower half of the inner cavity of the outer shell 1 is formed into a closed space by the turntable 5, the square plate 501 and the partition curtain 502.

[0038] In practical application, when the turntable 5 slides outward from the outer shell 1, the separator curtain 502, which is in a rolled-up state, is passively stretched, so that the lower half of the inner cavity of the outer shell 1 remains closed after the turntable 5 moves, thus preventing external pollutants from contaminating other culture dishes inside the outer shell 1; when the turntable 5 slides inward from the outer shell 1, the separator curtain 502 actively rolls up, thereby reducing the influence of the separator curtain 502 on the turntable 5 when it moves inside the outer shell 1.

[0039] like Figure 6 As shown, in another preferred embodiment of the present invention, the lower surface of the first baffle 611 and the upper surface of the turntable 5 are provided with magnetic plates with opposite magnetic properties.

[0040] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0041] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

[0042] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A constant-temperature cell culture device, comprising a shell (1), a door (2), a slide (3), a support (4), and a turntable (5), wherein the door (2) is installed at the opening of the shell (1), the slide (3) is installed inside the shell (1), the support (4) is installed on the slide (3), and the turntable (5) is installed in the middle of the support (4), characterized in that, Also includes: A lifting assembly (6) is mounted on a support column (4) for lifting and lowering the culture dish inside the turntable (5). The lifting assembly (6) includes an annular plate (61) fixedly connected to the support column (4). Multiple first supports (62) are mounted on the wall of the annular plate (61). Hollow toothed rings (63) are rotatably connected to the bottom of each of the first supports (62). A second support (64) is fixedly connected to the wall of the annular plate (61). A toothed ring plate (66) is slidably connected inside each of the multiple first supports (62). A first transmission rack (65) meshes with the inner wall of the toothed ring plate (66) is rotatably connected to the bottom of the second support (64). A rack (69) is fixedly connected to the bottom of the inner cavity of the outer shell (1). A third transmission rack (68) meshes with the rack (69) is rotatably connected inside the support column (4). A second transmission rack (67) is connected between the first transmission rack (65) and the third transmission rack (68). Clamping assembly (7) is set on turntable (5) for limiting and clamping the culture dish; A cell oscillation assembly (8) is disposed inside the outer shell (1) to prevent cell deposition in the culture dish; An air curtain assembly (9) is installed inside the housing (1) to prevent external environmental pollution of the interior of the housing (1).

2. The isothermal cell culture device according to claim 1, characterized in that, The hollow toothed ring (63) is provided with a groove inside that is adapted to the convex toothed ring plate (66). Among the multiple hollow toothed rings (63), the hollow toothed ring (63) closest to the opening of the outer shell (1) has a groove in the opposite direction to the other hollow toothed rings (63).

3. The isothermal cell culture device according to claim 1, characterized in that, Multiple loading racks (610) are slidably connected inside the turntable (5). A first spring (612) is fixedly connected between the bottom of the loading rack (610) and the lower surface of the turntable (5). A first baffle (611) is slidably connected to the side of the loading rack (610) near the culture dish.

4. The isothermal cell culture device according to claim 3, characterized in that, The clamping assembly (7) includes a support plate (71) fixedly connected to the bottom of the loading frame (610). A gear shaft (72) is rotatably connected to the top of the support plate (71). A second spring (73) is fixedly connected between the gear shaft (72) and the support plate (71). Clamping plates (74) are symmetrically slidably connected to the bottom of the loading frame (610). Both clamping plates (74) mesh with the gear shaft (72). A toothed plate (75) is slidably connected inside the loading frame (610) and below the clamping plates (74). A third spring (76) is fixedly connected between the toothed plate (75) and the inner wall of the loading frame (610). A push rod (77) is fixedly connected to the bottom of the turntable (5). The bottom of the push rod (77) is inclined and fits against the side of the toothed plate (75) away from the gear shaft (72).

5. The isothermal cell culture device according to claim 1, characterized in that, The cell oscillation assembly (8) includes an electrically driven shaft (81) mounted at the bottom of the inner cavity of the housing (1). A second shaft (86) is rotatably connected to the bottom of the inner cavity of the housing (1). A transmission belt (82) is drivingly connected between the second shaft (86) and the electrically driven shaft (81). An ultrasonic generator (87) is mounted on the top of the second shaft (86). Arc-shaped baffles (88) are symmetrically fixed to the side walls of the ultrasonic generator (87). The bottom of the inner cavity of the housing (1) and located at the second shaft (81) 6) is fitted with a shell (83) on the outside. A slide cylinder (84) is slidably connected inside the shell (83). A cylinder (89) is symmetrically fixedly connected to the inner wall of the slide cylinder (84). A fourth spring (85) is fixedly connected between the bottom of the slide cylinder (84) and the bottom of the inner cavity of the shell (1). A cover (810) is rotatably connected to the top of the slide cylinder (84). The cover (810) is slidably fitted on the ultrasonic generator (87). The cylinder (89) is attached to the surface of the arc-shaped baffle (88).

6. The isothermal cell culture device according to claim 5, characterized in that, The ultrasonic generator (87) generates ultrasonic waves vertically upwards, and when the cylinder (89) is not compressed by the arc-shaped baffle (88), the fourth spring (85) extends and pushes the cover (810) to abut against the bottom of the loading frame (610).

7. The isothermal cell culture device according to claim 1, characterized in that, The air curtain assembly (9) includes an air outlet chamber (91) fixedly connected to the top of the inner cavity of the outer shell (1). An air chamber (92) connected to the inside of the air outlet chamber (91) is fixedly connected to the top of the inner cavity of the outer shell (1). A sliding plate (93) is slidably connected inside the air chamber (92). A fifth spring (94) is fixedly connected between the sliding plate (93) and the inner wall of the air chamber (92). A round rod (95) is slidably connected on the slide (3). A sixth spring (96) is fixedly connected between the end of the round rod (95) and the slide (3). The bottom opening of the air outlet chamber (91) faces the opening of the outer shell (1) at a 45-degree angle.

8. The isothermal cell culture device according to claim 1, characterized in that, The turntable (5) is rotatably connected to a square plate (501) on its side wall. The square plate (501) is slidably connected to the inner cavity of the outer shell (1). A partition curtain (502) is fixedly connected to the bottom of the square plate (501). The side of the partition curtain (502) away from the square plate (501) is fixedly connected to the inner wall of the outer shell (1). The lower half of the inner cavity of the outer shell (1) is blocked by the turntable (5), the square plate (501) and the partition curtain (502) to form a closed space.

9. The isothermal cell culture device according to claim 3, characterized in that, The lower surface of the first baffle (611) and the upper surface of the turntable (5) are provided with magnetic plates with opposite magnetic properties.

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