Pseudomonas, bacterial agent containing the same, use thereof, and method for degrading polyamide

Abstract

This invention relates to the field of microbiology, and discloses a *Pseudomonas* bacterium, a bacterial agent containing the *Pseudomonas* bacterium, its applications, and a method for degrading polyamides. The *Pseudomonas* bacterium has the preservation number CCTCC NO: M 2026076. The *Pseudomonas* bacterium ZRZ-3 provided by this invention (… Pseudomonas sp. ZRZ-3 can grow using polyamide products as the sole carbon source, causing significant erosion and damage to the film surface and reducing the content of amorphous regions (referring to the preferential degradation of loosely structured areas in plastics that are easily attacked by microorganisms). This demonstrates that the strain can effectively degrade polyamide polymers, overcoming the shortcomings of existing technologies that can only degrade soluble monomers or oligomeric polyamides. At the same time, the degradation efficiency of this strain is significantly improved compared to existing technologies, and it has a broad-spectrum degradation ability for various types of polyamides and oligomers with different chain lengths, solving the problems of "low degradation efficiency and narrow degradation spectrum" in existing technologies.

Description

Technical Field

[0001] This invention relates to the field of microbiology, specifically to a Pseudomonas bacterium, a bacterial agent containing the Pseudomonas bacterium, its application, and a method for degrading polyamide. Background Technology

[0002] Polyamide (PA, commonly known as nylon), as an important synthetic polymer material, is widely used in textiles, engineering plastics, and packaging films due to its excellent mechanical properties, wear resistance, and chemical stability. However, the stable amide bonds in the polyamide structure make it extremely difficult for microorganisms to degrade it in the natural environment, leading to increasingly serious "white pollution" and microplastic problems caused by waste polyamide products. Traditional methods for treating waste polyamide mainly include landfill and incineration. However, landfill consumes a large amount of land resources, and polyamide can remain undecomposed for decades under landfill conditions; incineration produces harmful gases such as nitrogen oxides and dioxins, causing secondary pollution. Therefore, developing efficient biodegradation technologies that utilize microorganisms or their enzyme systems to decompose polyamide into smaller molecules or ultimately mineralize it into CO2 and H2O is considered an environmentally friendly and promising solution.

[0003] In the existing technology, there are a few research reports on microorganisms that degrade polyamide. For example, journal articles ( Journal of Bacteriology (1992, 174: 7948-7953) reported a strain of the genus Flavobacterium ( Flavobacterium A strain (sp.) was developed that degrades PA6 oligomers by secreting extracellular amidase, but this strain cannot degrade high molecular weight polyamide plastics. Another paper ( Journal of General Microbiology (1993, 139: 787-795) reported a strain of the genus *Pseudomonas* ( Pseudomonas sp. The ZRZ-3 strain degrades PA6 dimers by secreting 6-aminohexanoic acid dimer hydrolase (P-EII), but its degradation spectrum is narrow and it can only act on specific dimers.

[0004] In summary, the existing technology has the following drawbacks: (1) Most reported polyamide-degrading microorganisms can only act on soluble monomers or oligomers, and have virtually no ability to degrade high molecular weight polyamide plastic products (such as PA6, PA66 films or products); (2) Even the few strains that can act on polyamides have extremely low degradation efficiency and a long degradation cycle (weight loss rate <5% over several months), which cannot meet the needs of practical applications; (3) The degradation targets of existing strains are often limited to a specific dimer or oligomer, and cannot act on a broad spectrum of polyamides and their oligomers. Therefore, there is an urgent need in this field to develop microbial strains and their agents that can efficiently and broadly degrade polyamide plastic products. Summary of the Invention

[0005] The purpose of this invention is to overcome the problems of existing technologies, such as the inability of microbial strains to effectively degrade polyamide plastic products, low degradation efficiency, and narrow degradation spectrum. This invention provides a *Pseudomonas* bacterium, a bacterial agent containing this *Pseudomonas* bacterium, its application, and a method for degrading polyamide. The *Pseudomonas* bacterium provided by this invention can grow using polyamide plastic products as the sole carbon source, and exhibits particularly high efficiency and broad-spectrum degradation ability against PA6 films and various chain lengths of PA6 oligomers (including AHA, AHA3, AHA4, AHA5, and AHA6), while also possessing strong acid and alkali resistance.

[0006] To achieve the above objectives, the present invention provides, on the one hand, a strain of Pseudomonas ZRZ-3 ( Pseudomonas sp. ZRZ-3), the preservation number of the pseudomonad is CCTCC NO: M 2026076.

[0007] A second aspect of the present invention provides a microbial agent comprising Pseudomonas ZRZ-3 as described above. Pseudomonas sp. ZRZ-3).

[0008] A third aspect of the present invention provides the use of the aforementioned Pseudomonas bacteria and the aforementioned bacterial agents in the degradation of polyamide products.

[0009] A fourth aspect of the present invention provides a method for degrading polyamide, the method comprising contacting polyamide with the aforementioned Pseudomonas bacteria and / or the aforementioned bacterial agent.

[0010] Through the above technical solution, the present invention provides Pseudomonas ZRZ-3 ( Pseudomonas sp. ZRZ-3 can grow using polyamide plastic products as the sole carbon source, causing significant erosion and damage to the film surface and a decrease in the content of amorphous regions (referring to the preferential degradation of loosely structured areas in plastics that are easily attacked by microorganisms). This proves that the strain can effectively degrade high molecular weight polyamide plastic products, overcoming the shortcomings of existing technologies that "can only degrade soluble monomers or oligomers". At the same time, the degradation efficiency of this strain is significantly improved compared with existing technologies, and it has a broad-spectrum degradation ability for various polyamide types and oligomers with different chain lengths, solving the problems of "low degradation efficiency and narrow degradation spectrum" of existing technologies. It also has strong acid and alkali resistance.

[0011] Biological Preservation: The strain provided by this invention is classified and named Pseudomonas ( ). Pseudomonas sp. ZRZ-3 was deposited on January 12, 2026 at the China Center for Type Culture Collection (Address: Room 211, China Center for Type Culture Collection, Wuhan University, Wuchang District, Wuhan, Hubei Province, 430072, China; accession number: CCTCC NO: M2026076). Attached Figure Description

[0012] Figure 1 This is a growth curve of strain ZRZ-3 in Example 2 in a medium with PA6 film as the sole carbon source; Figure 2 These are scanning electron microscope (SEM) images of the PA6 film before and after degradation in Example 2; Figure 3 These are the X-ray diffraction (XRD) patterns of the PA6 film before and after degradation in Example 2; Figure 4 This is a graph showing the degradation effect of strain ZRZ-3 on PA6 pentamer in Example 7; Figure 5 This is a scanning electron microscope (SEM) image of PA6 film treated with *Pseudomonas putida* in Comparative Example 1 for 12 days. Figure 6 This is a graph showing the change in extracellular secretion production over time of strain ZRZ-3 and other candidate strains induced by PA6 oligomers in Example 2. Figure 7 This is a bar chart showing the extracellular enzyme activity of strain ZRZ-3 and other candidate strains 24 hours after the experiment in Example 2. Detailed Implementation

[0013] The endpoints and any values ​​of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.

[0014] The first aspect of this invention provides a strain of Pseudomonas ZRZ-3 ( Pseudomonas sp. ZRZ-3 The preservation number of the pseudomonad is CCTCC NO: M 2026076.

[0015] The Pseudomonas ZRZ-3 strain provided by this invention ( Pseudomonas sp. ZRZ-3 The strain can grow using polyamide plastic products as the sole carbon source, causing significant erosion and damage to the film surface and a decrease in the content of amorphous regions (referring to the preferential degradation of loosely structured areas in plastics that are easily attacked by microorganisms). This proves that the strain can effectively degrade high molecular weight polyamide plastic products, overcoming the shortcomings of existing technologies that "can only degrade soluble monomers or oligomers". At the same time, the degradation efficiency of this strain is significantly improved compared with existing technologies, and it has a broad-spectrum degradation ability for various polyamide types and oligomers with different chain lengths, solving the problems of "low degradation efficiency and narrow degradation spectrum" of existing technologies. It also has strong acid and alkali resistance.

[0016] The strain ZRZ-3 is classified as Pseudomonas ( ). Pseudomonas sp. ZRZ-3 was deposited on January 12, 2026 at the China Center for Type Culture Collection (Address: Room 211, China Center for Type Culture Collection, Wuhan University, Wuchang District, Wuhan, Hubei Province, 430072, China; accession number: CCTCC NO: M2026076).

[0017] A second aspect of the present invention provides a microbial agent comprising Pseudomonas as described above ( Pseudomonas sp. ZRZ-3).

[0018] According to the present invention, preferably, the microbial agent is a liquid microbial agent, a solid microbial agent, or a semi-solid microbial agent.

[0019] The microbial agent may contain a microbiologically acceptable carrier, preferably one or more of peat moss, bentonite, and diatomaceous earth. The weight ratio of the active ingredient (pseudomonas strain) to the carrier ranges from 1:1 to 10, preferably from 1:3 to 5. This range ensures a sufficiently high number of effective bacteria in the microbial agent while controlling costs and facilitating storage.

[0020] According to the present invention, preferably, when the bacterial agent is a liquid bacterial agent, the concentration of the Pseudomonas is 1×10⁻⁶. 9 CFU / mL or higher; when the bacterial agent is a solid bacterial agent, the concentration of the Pseudomonas is 1×10⁻⁶. 8 The inventors discovered in their research that using the above-mentioned ratio and concentration of microbial agents can significantly improve the degradation efficiency of polyamide plastic products while ensuring the storage stability and transportation convenience of the microbial agents. It is suitable for different application scenarios (such as liquid microbial agents for water treatment, and solid microbial agents for soil remediation and waste treatment), and has good prospects for industrial application.

[0021] A third aspect of this invention provides the application of the aforementioned Pseudomonas bacteria and the aforementioned bacterial agents in the degradation of polyamide products. The inventors discovered in their research that the strains and their agents of this invention can directly act on waste polyamide films, fibers, microplastics, and oligomers, initiating a biodegradation process at room temperature, thus providing an efficient biological tool for the harmless treatment of polyamide waste and the remediation of microplastic pollution.

[0022] According to the present invention, preferably, the polyamide is selected from at least one of PA6 and PA66; more preferably, PA6. The inventors have discovered that the strain of the present invention exhibits the best degradation effect on PA6 plastic products, can rapidly erode the surface of PA6 films and achieve molecular chain breakage, has a broad-spectrum degradation ability on PA6 oligomers (including AHA, AHA3, AHA4, AHA5, and AHA6), and also shows significant degradation activity on PA66 films. It is suitable for the removal of oligomers from nylon production wastewater and the biological treatment of waste nylon products.

[0023] A fourth aspect of this invention provides a method for degrading polyamide, the method comprising contacting polyamide with the aforementioned Pseudomonas bacteria and / or the aforementioned bacterial agent. The inventors have found that this method is simple to operate, operates under mild conditions, and does not require extreme conditions such as high temperature, high pressure, or strong acids and alkalis. It can effectively degrade polyamide under conventional environmental conditions, offering the technical advantages of low cost and no secondary pollution.

[0024] According to the present invention, preferably, the contact conditions include: a temperature of 4-42°C, a pH of 5-9, and a contact time of 10-15 days. The inventors discovered in their research that under these preferred conditions, the strain exhibits the highest growth activity and optimal degradation efficiency, significantly shortening the degradation cycle and making it suitable for process parameter control in industrial applications.

[0025] The present invention will be described in detail below through examples. In the following examples, PA6 and PA66 plastic films were purchased from Goodfellow Company, UK. All other raw materials and reagents were commercially available products.

[0026] The composition of LB medium is as follows: 10 g / L tryptone, 5 g / L yeast extract, 10 g / L NaCl, pH 7.0-7.2.

[0027] Composition and properties of the inorganic salt liquid culture medium: Na₂HPO₄ 2800 mg / L; KH₂PO₄ 1000 mg / L; (NH₄)₂SO₄ 500 mg / L; MgCl₂ 53 mg / L; Ca(NO₃)₂·4H₂O 50 mg / L; C 10 H 14 N2Na2O80.5 mg / L; FeSO4·7H2O0.2 mg / L; ZnSO4·7H2O 0.01 mg / L; MnCl2·4H2O 0.003 mg / L; H3BO30.03 mg / L; CoCl2·6H2O0.02 mg / L; CuCl2·2H2O 0.001 mg / L; NiCl2·6H2O 0.002 mg / L; H4MoNa2O60.003 mg / L; pH7.25.

[0028] Example 1: Isolation and Screening of Strains ZRZ-3 The target bacterial strain was isolated from polyamide-contaminated sediment samples using a strategy of "oligomer pre-enrichment - real plastic targeted screening". The specific steps are as follows: S1, enrichment culture Take 1 g of polyamide-contaminated sediment sample (oil sludge collected from Yangzi Petrochemical Company, Liuhe District, Nanjing City, Jiangsu Province, China), add it to 100 mL of inorganic salt liquid culture medium with PA6 oligomer mixture (containing AHA, AHA2-AHA6, total concentration approximately 500 mg / L) as the sole carbon source, and incubate at 30℃ and 220 rpm for 3 days with shaking. Take 1 mL of the above enriched solution and transfer it to fresh culture medium, continue incubating under the same conditions for 3 days, and repeat the transfer 10 times.

[0029] S2, Separation and Purification Take the third enrichment solution and perform a 10-fold serial dilution with sterile water (10... -1 -10 -6 Each dilution was plated onto LB agar plates and incubated at 30°C for 48 hours. Single colonies with different morphologies were picked and streaked three times on LB plates to obtain pure cultures, resulting in a total of 32 bacterial strains.

[0030] S3, Screening and Identification Thirty-two purified bacterial strains were inoculated into inorganic salt liquid medium containing a mixture of PA6 oligomers (total concentration approximately 500 mg / L) and cultured at 30°C and 220 rpm for 24 h with shaking. Uninoculated medium served as a control. After culture, the supernatant was collected by centrifugation, and the changes in the content of each oligomer were determined by HPLC. This process screened out strains that significantly reduced the content of AHA3-AHA6, resulting in a target strain named ZRZ-3.

[0031] S4. Strain Identification Genomic DNA was extracted from strain ZRZ-3, selected in step S3, and 16S rRNA gene amplification was performed by PCR (primers: 27F and 1492R). Sequencing yielded a 1450 bp sequence. BLAST alignment of the sequence in the GenBank database showed that this strain is related to the genus *Pseudomonas* (…). Pseudomonas Multiple strains of the bacteria showed homology exceeding 99%. Based on physiological and biochemical characteristics (Gram-negative, rod-shaped, oxidase-positive, and catalase-positive), it was identified as a new strain of the genus *Pseudomonas* and named *Pseudomonas* ZRZ-3. Pseudomonas sp. ZRZ-3).

[0032] The strain obtained in this embodiment is classified and named Pseudomonas ( ). Pseudomonas sp.ZRZ-3 was deposited on January 12, 2026 at the China Center for Type Culture Collection (Address: Room 211, China Center for Type Culture Collection, Wuhan University, Wuchang District, Wuhan, Hubei Province, 430072, China; accession number: CCTCC NO: M 2026076).

[0033] Example 2: Degradation performance test of strain ZRZ-3 on PA6 film The steps for testing the degradation ability of strain ZRZ-3 on PA6 plastic film in this embodiment are as follows: S1. Preparation of Experimental Materials Cut the PA6 film into 2 cm × 2 cm pieces (approximately 20 mg / piece), immerse in 75% ethanol for 30 minutes for disinfection, rinse three times with sterile water, dry and weigh (record as initial weight), and sterilize with ultraviolet light for later use.

[0034] S2, Experimental Methods The activated strain ZRZ-3 was inoculated into LB liquid medium and cultured at 30°C and 180 rpm for 24 hours with shaking. The bacterial cells were collected by centrifugation, washed twice with sterile physiological saline, resuspended in sterile physiological saline, and the OD was adjusted. 600 Up to 0.5 (approximately 10) 8 (CFU / mL), as a seed solution.

[0035] The seed culture was inoculated at a rate of 2% by volume into an inorganic salt liquid culture medium with PA6 film as the sole carbon source (2 PA6 films per 100 mL of medium). Initial OD 600 Approximately 0.1. Cultured at 30℃, pH 6, and with shaking at 180 rpm for 12 days. A control culture medium without inoculation was provided. Each group was divided into three replicates.

[0036] S3, Determination of bacterial growth Culture medium samples were taken every two days to measure OD. 600 Value. Result as follows Figure 1 As shown, the OD of strain ZRZ-3 600 The value increased by 0.12 over 12 days, while the OD value in the control group increased by 0.12. 600 The value remained around 0.1. The results indicate that strain ZRZ-3 can grow using PA6 film as the sole carbon source, demonstrating its ability to utilize PA6 film.

[0037] S4. Observation of film surface morphology After the culture was completed, the PA6 film was removed and soaked overnight at 4°C in 2% glutaraldehyde solution to fix the bacteria on the film surface. After rinsing with phosphate buffer and drying, it was observed by SEM.

[0038] SEM observation results are as follows: Figure 2 As shown: the control group had a smooth, flat film surface without bacteria; while the film surface treated with strain ZRZ-3 for 12 days showed a large number of aggregated bacteria, dense erosion holes and cracks, indicating that the strain caused significant damage to the film and its surface.

[0039] S5, X-ray diffraction (XRD) data analysis XRD analysis was performed on PA6 film samples after 12 days of degradation treatment and undegraded control film samples. The results are as follows: Figure 3 As shown, the degradation effect of strain ZRZ-3 on PA6 film occurs not only in the amorphous region but also in the crystalline region, leading to a decrease in the overall crystallinity of the film. This is corroborated by the surface morphology changes and weight loss data observed by SEM, further confirming the effective degradation ability of strain ZRZ-3 on PA6 plastic.

[0040] S6, Weightlessness Test The weight loss rate of PA6 film samples after 12 days of degradation treatment and undegraded control film samples was measured. The PA6 films were removed, ultrasonically cleaned with SDS solution, rinsed with sterile water, and dried. They were then accurately weighed using an electronic balance (recorded as the weight after degradation). The weight loss rate was calculated using the following formula: Weight loss rate (%) = (Initial weight - Weight after degradation) / Initial weight × 100%. The results showed that after 12 days of treatment with strain ZRZ-3, the average weight loss rate of the PA6 film reached 6.54%, while the control film showed no significant change in weight (weight loss rate was only 0.5%). This indicates that strain ZRZ-3 can significantly degrade the bulk structure of the PA6 film, achieving effective weight reduction.

[0041] S6. Determination of bacterial secretion capacity To further verify the degradation mechanism of polyamide by strain ZRZ-3, the extracellular secretion yield of each candidate strain was measured. The 32 candidate strains obtained from step S3 of Example 1 (including...) were used. Pseudomonas、 Stenotrophomonas、Pedobacter、Bordetella、Achromobacter Strains of the same genus were inoculated into inorganic salt liquid medium containing a mixture of PA6 oligomers and cultured at 30°C with shaking at 220 rpm. Samples were taken at 0 h, 4 h, 8 h, 12 h, 16 h, 20 h, 24 h, 28 h, and 32 h, and the absorbance (OD) of the culture medium at 280 nm was measured. 280 The relative content of extracellular secretions (mainly proteins) was characterized. Uninoculated culture medium served as a control. Three replicates were set up for each group.

[0042] The results are as follows Figure 6 As shown, the secretion yield of each strain exhibits different trends with increasing culture time: control group (no inoculation) OD280 The value remained around 0.1, with no significant change; strain ZRZ-3 ( Pseudomonas sp. ZRZ-3) OD 280 The value increases slowly in the early stage of culture (0-8 h), and enters a rapid secretion phase after 8 h, showing a continuous upward trend; Stenotrophomonas、Pedobacter、Bordetella、Achromobacter While the secretion production of other strains also increased, the overall level was significantly lower than that of strain ZRZ-3. These results indicate that strain ZRZ-3 can secrete large amounts of extracellular products (presumably degradation-related enzymes) under PA6 oligomer induction, with significantly higher secretion levels than strains of other genera, and this secretion continues to increase with prolonged culture time. This is consistent with its highly efficient degradation ability of PA6 oligomers (step S7), further confirming the mechanism by which strain ZRZ-3 degrades polyamides through the secretion of extracellular active substances.

[0043] S7, Enzyme Activity Assay To further verify the degradation mechanism of PA6 film by strain ZRZ-3, the activity of extracellular enzymes secreted by the strain during culture was measured.

[0044] The results are as follows Figure 7 As shown, strain ZRZ-3 ( Pseudomonas sp. ZRZ-3 strain showed significantly better degradation of PA6 oligomers than other strains: after 24 hours of cultivation, AHA3 content decreased by 11.23%, AHA4 content by 9.85%, AHA5 content by 9.65%, and AHA6 content by 2.42%; while Stenotrophomonas , Pedobacter , Bordetella , Achromobacter The degradation rates of each oligomer by strain ZRZ-3 were all below 5%. These results indicate that strain ZRZ-3 possesses a highly efficient extracellular enzyme secretion system, enabling it to rapidly degrade PA6 oligomers of various chain lengths. This further confirms the mechanism by which strain ZRZ-3 achieves broad-spectrum degradation of polyamides through the secretion of highly active extracellular enzymes.

[0045] In summary, strain ZRZ-3 exhibits significant degradation capabilities for high molecular weight PA6 plastic films, demonstrating a synergistic effect across multiple dimensions, including cell growth, surface erosion, crystallization disruption, weight reduction, secretion production, and enzyme activity. It also demonstrates a short degradation cycle (6.54% weight loss in 12 days) and high enzyme activity, showing significantly better performance than strains reported in existing technologies.

[0046] Example 3 The degradation performance was tested according to the method in Example 2, except that the PA6 film was replaced with a PA66 film. After 15 days of cultivation, SEM observation showed obvious erosion marks and microcracks on the surface of the treated film, indicating that strain ZRZ-3 also has the ability to degrade PA66 film. The results of this example show that strain ZRZ-3 can degrade not only PA6 but also PA66, further confirming its broad-spectrum degradation characteristics.

[0047] Example 4 The degradation performance was tested according to the method in Example 2, except that the pH was adjusted to 4. After culturing for 15 days, SEM observation showed that after treatment with strain ZRZ-3 at pH=4 for 15 days, a certain degree of erosion traces appeared on the surface of the PA6 film, with a small number of micropores and increased surface roughness. However, the degree of erosion was weaker than that of the treatment group described in Example 2. The results of this example show that strain ZRZ-3 can still maintain its ability to degrade PA6 film under acidic conditions of pH=4, proving that the strain has good acid resistance and can be used for the biodegradation of polyamide materials in acidic environments.

[0048] Example 5 The degradation performance was tested according to the method in Example 2, except that the pH was adjusted to 9. After culturing for 15 days, SEM observation showed that after treatment with strain ZRZ-3 at pH=9 for 15 days, visible erosion marks appeared on the surface of the PA6 film, the surface roughness increased, and a small number of microcracks appeared. However, the degree of erosion was weaker than that of the treatment group described in Example 2. The results of this example show that strain ZRZ-3 can still maintain its ability to degrade PA6 film under alkaline conditions at pH=9, proving that the strain has good alkali resistance and can be used for the biodegradation of polyamide materials in alkaline environments.

[0049] Example 6 The degradation performance was tested according to the method in Example 2, except that the PA6 film was replaced with a laboratory-synthesized PA6 oligomer (pentamer). The results are as follows. Figure 4 As shown, after 24 hours of culture, strain ZRZ-3 achieved a degradation rate of 10.23% for PA6 pentamers; after 168 hours of culture, the degradation rate reached 100%, and the pentamers were completely degraded. No significant pentamer degradation was detected in the control group throughout the entire culture period.

[0050] The results of this example show that strain ZRZ-3 can efficiently degrade PA6 pentamers, with a degradation rate of 10.23% in 24 hours and complete degradation in 168 hours, further confirming the broad-spectrum degradation ability of strain ZRZ-3 for PA6 oligomers.

[0051] Comparative Example 1 The degradation performance was tested according to the method in Example 2, except that the *Pseudomonas* strain with accession number CCTCC NO: M 2026076 provided by this invention was replaced with *Pseudomonas putida* (purchased from Guangdong Provincial Center for Microbiological Culture Collection). The results showed that the OD of this strain... 600 The value increased from 0.1 to 0.12, an increase of only 0.02; SEM observation showed... Figure 5 As shown, there are no obvious signs of erosion on the surface of the film.

[0052] The results of this comparative example show that the Pseudomonas strains reported in the prior art have virtually no ability to degrade high molecular weight PA6 films, which is in stark contrast to the degradation effect of the strain ZRZ-3 of this invention.

[0053] The preferred embodiments of the present invention have been described in detail above; however, the present invention is not limited thereto. Within the scope of the inventive concept, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as the content disclosed in the present invention and are all within the protection scope of the present invention.

Claims

1. A strain of Pseudomonas ZRZ-3 ( Pseudomonas sp. ZRZ-3), characterized in that, The preservation number of the Pseudomonas is CCTCC NO: M 2026076.

2. A microbial agent, characterized in that, The bacterial agent contains Pseudomonas ZRZ-3 as described in claim 1 ( Pseudomonas sp. ZRZ-3).

3. The microbial agent according to claim 2, characterized in that, The microbial agent can be a liquid microbial agent, a solid microbial agent, or a semi-solid microbial agent.

4. The microbial agent according to claim 3, characterized in that, When the bacterial agent is a liquid bacterial agent, the concentration of the Pseudomonas is 1×10⁻⁶. 9 CFU / mL or higher.

5. The microbial agent according to claim 3, characterized in that, When the bacterial agent is a solid bacterial agent, the concentration of the Pseudomonas is 1×10⁻⁶. 8 CFU / g or higher.

6. The use of the Pseudomonas aeruginosa as described in claim 1, or the bacterial agent as described in any one of claims 2-5, in the degradation of polyamide products.

7. The application according to claim 6, characterized in that, The polyamide is selected from at least one of PA6, PA66 and PA11.

8. The application according to claim 7, characterized in that, The polyamide is PA6.

9. A method for degrading polyamide, characterized in that, The method includes reacting the Pseudomonas aeruginosa of claim 1 and / or the bacterial agent of any one of claims 2-5 with polyamide.

10. The method according to claim 9, characterized in that, The contact conditions include: a temperature of 4-42°C, a pH of 4-9, and a contact time of 10-15 days.

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