A device for cultivating lactarius deliciosus using pine bark and a method of using the same

Abstract

The present application relates to a device for cultivating Lactarius deliciosus by using the bark of pine tree and its using method, belonging to the field of edible mushroom cultivation. The present application solves the problem that Lactarius deliciosus is rarely grown on the bark of pine tree under natural conditions due to lack of spore source, moisture environment, water source and ventilation condition. The device comprises a water bucket (1), a spore cloth (7), a water-absorbing cloth (11) and a water-repellent cloth (14). The water bucket (1) provides water source, the spore cloth (7) is inoculated with Lactarius deliciosus spores, the water-absorbing cloth (11) supplies water source and maintains humidity, and the water-repellent cloth (14) blocks wind and reduces water evaporation. The using method is as follows: preparing LBLC culture medium, i.e. 20 g of potato, 2 g of glucose, 1 g of KHPO4, 1 g of KH2PO4, 1 g of thiamine and 1000 ml of water; winding the spore cloth (7), the water-absorbing cloth (11) and the water-repellent cloth (14); and binding the water bucket (1). The present application is used for cultivating Lactarius deliciosus on the bark of pine tree.

Description

Technical Field

[0001] This invention relates to the field of promoting the propagation of wild edible fungi, and more particularly to the field of promoting the propagation of wild Lactarius deliciosus, specifically to a device and method for cultivating Lactarius deliciosus using pine bark. Background Technology

[0002] The milk cap mushroom (Lactarius deliciosus) can live in symbiosis with pine trees. Under natural conditions, it commonly grows in pine forests, and can also grow on the bark of pine trunks, but this is extremely rare. Current technology lacks the equipment and methods for cultivating milk cap mushrooms on pine trunk bark. Summary of the Invention

[0003] The fact that *Lactarius deliciosus* can grow on pine bark under natural conditions indicates that pine bark has the necessary conditions for its growth. However, the extremely rare occurrence of *Lactarius deliciosus* growing on pine bark under natural conditions suggests that it is difficult to find all the necessary conditions for its growth in nature.

[0004] Through long-term investigation and research, the inventor discovered that under the following conditions, the fruiting bodies of *Lactarius deliciosus* can also grow on the bark of pine trunks: mycelium supply, a continuously high humidity microenvironment, a slow and clean water supply, a breathable but not ventilated microenvironment, pine biomass nutrition supply, suitable temperature, and thunderstorms promoting mushroom bud germination.

[0005] Clearly, under natural conditions, pine bark lacks the necessary conditions for cultivating *Lactarius deliciosus* (pine lactea) mycelium, a continuously high-humidity microenvironment, a slow and clean water supply, and a breathable but not well-ventilated microenvironment. Existing literature does not report any technical inventions for cultivating *Lactarius deliciosus* using pine bark. To overcome these shortcomings, this invention provides a device and method for cultivating *Lactarius deliciosus* using pine bark.

[0006] The technical solution adopted by the present invention to solve its technical problem is: a device for cultivating pine milkvetch using pine bark and its method of use, characterized in that: the device for cultivating pine milkvetch using pine bark includes a water bucket (1), a spawn cloth (7), an absorbent cloth (11), and a water-repellent cloth (14), wherein the water bucket (1) is used to provide a water source, the water bucket (1) is provided with a handle (2) for carrying the water bucket (1), the water bucket (1) is provided with a lid (3) for covering the water bucket (1), and the water bucket (1) is provided with a small ring. (4) and rope (5) are used to tie and fix the bucket (1) to the pine trunk (6). The inoculum cloth (7) is used to inoculate the bark of the pine trunk (6) with the fungal inoculum. The inoculum cloth (7) is provided with a first elastic cloth (8) for wrapping the inoculum cloth (7) around the pine trunk (6). The inoculum cloth (7) is provided with a first Velcro (9) for wrapping, bonding and fixing the first elastic cloth (8) around the pine trunk (6). The absorbent cloth (11) is used to continuously and slowly supply water and maintain humidity. 11) A second elastic fabric (12) is provided for wrapping the absorbent fabric (11) around the pine trunk (6). The absorbent fabric (11) is provided with a second Velcro fastener (13) for wrapping, bonding, and fixing the second elastic fabric (12) around the pine trunk (6). The hydrophobic fabric (14) is used for wind protection, light blocking, preventing disturbance, reducing moisture evaporation, and keeping the bark of the pine trunk (6) moist. The hydrophobic fabric (14) is provided with a third elastic fabric (15) for wrapping the hydrophobic fabric (14) around the pine trunk (6). The third Velcro (16) is used to wrap, bond, and fix the third elastic fabric (15) to the pine trunk (6). The absorbent fabric (11) is a strip of fabric made of polyester fiber and viscose fiber. The spawn fabric (7) is a strip of fabric made of polyester fiber and viscose fiber. The pine lactus spawn is inoculated with immersion medium (LBLC) and taken out after being cultivated at 28°C for 7 days. The hydrophobic fabric (14) is a fabric made of hydrophobic fiber. The elastic fabric (8, 12, 15) is a raw fabric with elastic function.The device for cultivating *Lactarius deliciosus* using pine bark is characterized by the following method: a. Preparing a culture medium (LBLC): 20g potato, 2g glucose, 1g KHPO4, 1g KH2PO4, 1g thiamine, and 1000ml water; b. Sterilizing the prepared LBLC culture medium at 115℃ for 20 minutes and pouring it into a glass petri dish (10); c. Soaking the spawn cloth (7) in the sterilized LBLC culture medium and then inoculating it with *Lactarius deliciosus* spawn; d. Cultivating at 28℃ for 7 days; e. Using a first elastic cloth (8) and a first Velcro (9), wrapping the spawn cloth (7) with the cultivated spawn onto the pine bark (6). f. Using the second elastic fabric (12) and the second Velcro (13), wrap the absorbent fabric (11) around the top of the pine trunk (6) and the top of the wrapped fungal cloth (7); g. Using the third elastic fabric (15) and the third Velcro (16), wrap the hydrophobic fabric (14) around the top of the pine trunk (6) and the top of the wrapped absorbent fabric (11); h. Use the small ring (4) and the rope (5) to tie and fix the bucket (1) to the pine trunk (6) above the wrapped hydrophobic fabric (14); i. Stuff the strip of cloth above the absorbent fabric (11) into the water in the bucket (1); j. Put the lid (3) on the bucket (1).

[0007] The names and uses of each component are as follows:

[0008] Bucket (1): Used to provide water.

[0009] Handle (2): Used for carrying bucket (1).

[0010] Bucket lid (3): Used to cover the bucket (1).

[0011] Small ring (4): Used to work with rope (5) to wrap and secure the bucket (1) to the pine trunk (6).

[0012] Rope (5): Used in conjunction with small ring (4) to wrap and secure the bucket (1) to the pine trunk (6).

[0013] Pine trunk (6): Used to establish a symbiotic system between pine trees and Lactobacillus pineae to promote the growth of Lactobacillus pineae on the bark of pine trunk (6).

[0014] Inoculation cloth (7): Used for inoculating the bark of pine trunks (6) with *Lactobacillus pineensis* fungus.

[0015] First elastic cloth (8): used to wrap the inoculum cloth (7) onto the pine trunk (6).

[0016] First magic (9): used to wrap, glue and fix the first elastic cloth (8) to the pine trunk (6).

[0017] Glass petri dishes (10): used for aseptic operations such as preparing culture medium (LBLC), inoculation, sterilization, and making inoculum cloth (7).

[0018] Absorbent cloth (11): Used for a continuous, slow supply of water and to maintain humidity.

[0019] Second elastic fabric (12): used to wrap the absorbent fabric (11) around the pine trunk (6).

[0020] Second magic trick (13): used to wrap, glue and fix the second elastic cloth (12) to the pine trunk (6).

[0021] Hydrophobic fabric (14): used for wind protection, shading, preventing disturbance, reducing moisture loss, and maintaining the moisture content of the pine trunk (6) surface.

[0022] Third elastic fabric (15): used to wrap the hydrophobic fabric (14) around the pine trunk (6).

[0023] The third magic trick (16): used to wrap, glue and fix the third elastic cloth (15) to the pine trunk (6).

[0024] In nature, liquid water flows downwards under the influence of gravity; fungal spores are suspended in the air and easily fall to the ground; even if they do land on pine bark, they are easily blown away by the wind; fungal hyphae spread and elongate outwards; tree trunks are exposed to considerable wind and rain; pine roots primarily grow deep into the soil; and the mycelium of the pine lacteus fungus can coexist with pine trees, producing the edible wild mushroom pine lacteus fruiting body under suitable conditions. Therefore, while pine lacteus can grow on pine bark naturally, it is difficult due to a lack of pine lacteus spores, a lack of a high-humidity microenvironment, a lack of a long-term, slow-flowing water supply, and a lack of a well-ventilated but poorly ventilated microenvironment.

[0025] It is difficult for *Lactarius deliciosus* mycelium to grow and spread on the bark of pine trees in nature. This invention solves the problem of inoculating *Lactarius deliciosus* mycelium on the bark of pine trees. The bark of pine trees in nature lacks a consistently high-humidity microenvironment. This invention solves the problem of maintaining a high-humidity environment on the bark of pine trees. Water in nature cannot flow upwards, nor can it flow continuously along the trunk for several days. Therefore, it is difficult for water to accumulate on the surface of pine trees. This invention solves the problem of retaining moisture on the bark of pine trees. It is difficult to maintain a breathable but not well-ventilated microenvironment on the bark of pine trees in nature. This invention solves the problem of maintaining a breathable but not well-ventilated microenvironment on the bark of pine trees.

[0026] In nature, pine roots can extend into the soil and coexist with *Lactarius deliciosus* to produce fruiting bodies. Sometimes, *Lactarius deliciosus* fruiting bodies can be found on leaf-covered ground, indicating that sunlight is not a necessary condition for *Lactarius deliciosus*. This invention establishes a symbiotic fruiting system between *Lactarius deliciosus* mycelium and pine bark. Under natural conditions, pine bark lacks conditions such as "spores, humidity, water source, and poor ventilation," but these conditions are easily met artificially. This is the innovative aspect of this invention. By artificially providing these conditions and establishing a symbiotic system between *Lactarius deliciosus* mycelium and pine bark, this invention promotes the growth of *Lactarius deliciosus* fruiting bodies on pine bark, thus meeting people's edible requirements for *Lactarius deliciosus*.

[0027] The primordium, or embryonic organoid, is formed from physiologically mature mycelium through kinking differentiation. Primordium formation generally requires suitable light, oxygen, low temperature, thiamine, and other external factors, including physical, chemical, and biological stimuli. The culture medium (LBLC) formulation is also a formulation designed based on actual conditions in this invention, and is one of the inventive aspects of this invention.

[0028] Because pine trunks stand exposed to the air, it's difficult for *Lactarius deliciosus* spores to grow on the bark naturally. This invention solves this problem. Also, because pine trunks stand exposed to the air, it's difficult to maintain a consistently high humidity environment on the bark naturally. This invention solves this problem. Furthermore, because pine trunks stand exposed to the air, any water runoff will reach the roots naturally, making it difficult to maintain a continuous supply of clean water to the bark naturally. This invention solves this problem. Finally, because pine trunks stand exposed to the air, airflow is unfavorable for fungal growth and development, making it difficult to maintain a breathable but not well-ventilated microenvironment on the bark naturally. This invention solves this problem.

[0029] The beneficial effects of this invention are:

[0030] ① Provide the pine lactus fungus strain, high humidity microenvironment, clean water source, and breathable but not ventilated microenvironment to the bark of pine trunk (6).

[0031] ② The designed culture medium (LBLC) provides a supply of thiamine, which is beneficial for the formation of primordia.

[0032] ③ The designed culture medium (LBLC) provides potassium and phosphorus, which is beneficial to the growth of Lactobacillus pineae.

[0033] ④ The technique of inoculating *Lactarius deliciosus* with absorbent cloth strips soaked in culture medium (LBLC) solves the problem of the lack of strain source under natural conditions.

[0034] ⑤ The water-absorbing cloth strip water supply technology solves the defects of natural water sources.

[0035] ⑥ The hydrophobic fabric apron moisturizing technology solves the problem of moisture retention under natural conditions.

[0036] ⑦ Element addition technology addresses nutritional deficiencies caused by natural conditions.

[0037] ⑧ The cloth strip design provides both inoculation and water supply to the entire pine trunk (6) and prevents mushrooms from growing. Attached Figure Description

[0038] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0039] Figure 1 This is a schematic diagram of a partial embodiment of an apparatus for cultivating Lactarius deliciosus using pine bark, which is part of the present invention.

[0040] In the picture, 1. Bucket, 2. Handle, 3. Bucket lid, 4. Small ring, 5. Rope, 6. Pine trunk, 7. Spawn cloth, 8. First elastic cloth, 9. First magic trick, 10. Glass petri dish, 11. Absorbent cloth, 12. Second elastic cloth, 13. Second magic trick, 14. Hydrophobic cloth, 15. Third elastic cloth, 16. Third magic trick. Detailed Implementation

[0041] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0042] In the description of this invention, LBLC medium is an abbreviation of the first letters of the main English words "Liu Baoping designed Lactarius deliciosus(L)Gray CultureMedium".

[0043] In some literature, "Lactarius deliciosus" is a general term for this species, mycelium, and mushroom, which is easily confused. In the description of this invention, it should be understood that when the term "Lactarius deliciosus" appears alone without the prefix "mycelium," it refers to the fruiting body of *Lactarius deliciosus*, this edible wild mushroom. It should also be understood that the terms "above," "above," "below," "adjacent," and "in water," etc., indicate the orientation or positional relationship based on the perspective of an eyewitness facing the invention in a virtual installation scenario. These terms are used only for ease of description and simplification and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention. The water-absorbing fabric strip mycelium supply technology, water source supply technology, hydrophobic apron moisturizing technology, and breathable-but-non-ventilated creation technology for establishing a symbiotic system between pine trunk bark and *Lactarius deliciosus* are all within the scope of protection of this invention.

[0044] The magic tricks (9, 13, 16) in the diagram, where they are bonded after wrapping, are a conventional technique. It should also be understood that the drawing only shows one surface; the magic tricks (9, 13, 16) in the attached diagram are for illustrative purposes only and should not be construed as a difference in the actual invention. In the description of this invention, the shape, structure, size, material, etc., of components not specifically described in detail adopt commonly known standard technical specifications. Other technical details are implemented in accordance with generally known industry standards.

[0045] Example 1

[0046] A device for cultivating *Lactarius deliciosus* using pine bark and its method of use, characterized in that: the device for cultivating *Lactarius deliciosus* using pine bark includes a water bucket (1), a spawn cloth (7), an absorbent cloth (11), and a water-repellent cloth (14). The water bucket (1) is used to provide a water source. The water bucket (1) is equipped with a handle (2) for carrying the water bucket (1). The water bucket (1) is equipped with a lid (3) for covering the water bucket (1). The water bucket (1) is equipped with a small ring (4) and a rope (5) for binding and fixing the water bucket (1) to the pine bark (6). The spawn cloth (7) is used to fertilize the pine bark (6). The surface of the fungus *Lactarius deliciosus* is inoculated with a spore cloth (7). The spore cloth (7) is provided with a first elastic cloth (8) for wrapping the spore cloth (7) around the pine trunk (6). The spore cloth (7) is provided with a first Velcro fastener (9) for wrapping, bonding, and fixing the first elastic cloth (8) to the pine trunk (6). The absorbent cloth (11) is used to continuously and slowly supply water and maintain humidity. The absorbent cloth (11) is provided with a second elastic cloth (12) for wrapping the absorbent cloth (11) around the pine trunk (6). The absorbent cloth (11) is provided with a second Velcro fastener (13) for wrapping and bonding the second elastic cloth (12). The hydrophobic cloth (14) is fixed to the pine trunk (6). It is used to block wind, shade, prevent disturbance, reduce water evaporation, and keep the bark of the pine trunk (6) moist. The hydrophobic cloth (14) is provided with a third elastic cloth (15) for wrapping the hydrophobic cloth (14) to the pine trunk (6). The hydrophobic cloth (14) is provided with a third Velcro (16) for wrapping, bonding, and fixing the third elastic cloth (15) to the pine trunk (6). The absorbent cloth (11) is a strip of cloth made of polyester fiber and viscose fiber. The inoculum cloth (7) is a strip of cloth made of polyester fiber and viscose fiber. The spores of *Lactarius deliciosus* were inoculated on the immersion medium (LBLC) and incubated at 28°C for 7 days before being removed. The hydrophobic cloth (14) is a fabric made of hydrophobic fibers. The elastic cloth (8, 12, 15) is a raw cloth with elastic function. The width of the spore cloth (7), the absorbent cloth (11), and the hydrophobic cloth (14) is 4 cm. The length of the spore cloth (7) is 100 cm. The length of the absorbent cloth (11) is 160 cm. The length of the hydrophobic cloth (14) is 120 cm. The width of the elastic cloth (8, 12, 15) is 4 cm and the length is 80 cm. The lower diameter of the glass petri dish (10) is 200 mm.The device for cultivating *Lactarius deliciosus* using pine bark is characterized by the following method: a. Preparing a culture medium (LBLC): 20g potato, 2g glucose, 1g KHPO4, 1g KH2PO4, 1g thiamine, and 1000ml water; b. Sterilizing the prepared LBLC culture medium at 115℃ for 20 minutes and pouring it into a glass petri dish (10); c. Soaking the spawn cloth (7) in the sterilized LBLC culture medium and then inoculating it with *Lactarius deliciosus* spawn; d. Cultivating at 28℃ for 7 days; e. Using a first elastic cloth (8) and a first Velcro (9), wrapping the spawn cloth (7) with the cultivated spawn onto the pine bark (6). f. Using the second elastic fabric (12) and the second Velcro (13), wrap the absorbent fabric (11) around the top of the pine trunk (6) and the top of the wrapped fungal cloth (7); g. Using the third elastic fabric (15) and the third Velcro (16), wrap the hydrophobic fabric (14) around the top of the pine trunk (6) and the top of the wrapped absorbent fabric (11); h. Use the small ring (4) and the rope (5) to tie and fix the bucket (1) to the pine trunk (6) above the wrapped hydrophobic fabric (14); i. Stuff the strip of cloth above the absorbent fabric (11) into the water in the bucket (1); j. Put the lid (3) on the bucket (1).

Claims

1. A device for cultivating *Lactarius deliciosus* using pine bark and its method of use, characterized in that: An apparatus for cultivating *Lactarius deliciosus* using pine bark includes a water bucket (1), a spawn cloth (7), an absorbent cloth (11), and a water-repellent cloth (14). The water bucket (1) provides a water source, has a handle (2) for carrying, a lid (3) for covering, and small rings (4) and ropes (5) for binding and securing the bucket (1) to the pine trunk (6). The spawn cloth (7)... For inoculating the bark of pine trunks (6) with *Lactarius deliciosus* fungus, the inoculum cloth (7) is provided with a first elastic cloth (8) for wrapping the inoculum cloth (7) onto the pine trunk (6), and a first Velcro fastener (9) for wrapping, bonding, and fixing the first elastic cloth (8) onto the pine trunk (6). The absorbent cloth (11) is used to continuously and slowly supply water and maintain humidity, and the absorbent cloth (11) is provided with a second elastic cloth (12) for wrapping the absorbent cloth (11) with... On the pine trunk (6), the absorbent cloth (11) is provided with a second Velcro (13) for wrapping, bonding, and fixing the second elastic cloth (12) to the pine trunk (6). The hydrophobic cloth (14) is used to block wind, shade, prevent disturbance, reduce water evaporation, and keep the bark of the pine trunk (6) moist. The hydrophobic cloth (14) is provided with a third elastic cloth (15) for wrapping the hydrophobic cloth (14) to the pine trunk (6). The hydrophobic cloth (14) is provided with a third Velcro (16) for... The third elastic cloth (15) is wrapped, bonded and fixed to the pine trunk (6). The absorbent cloth (11) is a strip of cloth made of polyester fiber and viscose fiber. The spawn cloth (7) is a strip of cloth made of polyester fiber and viscose fiber. The pine lactus spawn is inoculated with immersion medium (LBLC) and taken out after being cultivated at 28°C for 7 days. The hydrophobic cloth (14) is a fabric made of hydrophobic fiber. The elastic cloth (8, 12, 15) is a raw cloth with elastic function.

2. The apparatus for cultivating *Lactarius deliciosus* using pine bark according to claim 1, characterized in that: The method of use is as follows: a. Prepare the culture medium (LBLC): 20g potato, 2g glucose, 1g KHPO4, 1g KH2PO4, 1g thiamine, 1000ml water; b. Sterilize the prepared LBLC culture medium at 115℃ for 20 minutes and pour it into a glass petri dish (10); c. Soak the spawn cloth (7) in the sterilized LBLC culture medium, and then inoculate it with the pine lactus spawn; d. Cultivate at 28℃ for 7 days; e. Wrap the spawn cloth (7) with the first elastic cloth (8) and the first Velcro (9) to the pine trunk (6); f. Wrap the absorbent cloth (11) with the second elastic cloth (12) and the second Velcro (13). g. Wrap the hydrophobic cloth (14) around the top of the pine trunk (6) and the top of the wrapped fungal cloth (7); h. Use the third elastic cloth (15) and the third Velcro (16) to wrap the hydrophobic cloth (14) around the top of the pine trunk (6) and the top of the wrapped absorbent cloth (11); h. Tie and fix the bucket (1) to the pine trunk (6) above the wrapped hydrophobic cloth (14) with the small ring (4) and the rope (5); i. Stuff the strip of cloth above the absorbent cloth (11) into the water in the bucket (1); j. Put the bucket lid (3) on the bucket (1).

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