A method for efficient propagation of plants by long-beaked morular cells

Abstract

The application relates to a method for efficiently propagating plants through long-beaked thick-embryonic cells, and belongs to the technical field of plant tissue culture. The method comprises five steps of embryonic cell induction, proliferation and maintenance, somatic embryo differentiation and maturation, germination and seedling growth and domestication and transplanting. By optimizing the sucrose concentration in the induction medium to 50 g / L and adopting the technology of combining cell cluster sorting with suspension culture, the induction rate and the proliferation speed of the embryonic cells are significantly improved; by controlling the inoculation density and adding glutamine and abscisic acid in combination during the somatic embryo differentiation stage, about 67 cotyledon embryos can be generated from 200 cell clusters; during the germination stage, by synergistically using gibberellin and Karrikin 1, the synchronous development of roots and stems is promoted, and robust seedlings with multiple true leaves and multiple main roots are obtained. The application first realizes the large-scale propagation of about 6800 somatic embryo seedlings from 1 gram of embryonic cells, and the transplanting survival rate reaches 96.2%, thereby providing an innovative technical scheme for the germplasm preservation and efficient propagation of long-beaked Magnolia officinalis.

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