Artificial breeding method of leptobramis modestus and hatching equipment thereof

Abstract

The present application relates to a kind of artificial propagation method of Leucoglanis guichenoti.The artificial propagation efficiency of Leucoglanis guichenoti in prior art is low, and the rate of induced spawning, fertilization rate and hatching rate are not high.The propagation method of the present application comprises: using induced spawning agent (water temperature 13-19℃) to Leucoglanis guichenoti parent fish; the parent fish is put into spawning and hatching frame to spawn and fertilize naturally; after taking out the parent fish, keep the water level 5-15cm, and flow water hatching with 2-4 times / day exchange amount to obtain membrane fry; after another 24h culture, flat swimming fry is obtained.The induced spawning agent contains LRH-A2 and DOM, the dosage of female fish is LRH-A2 3-5ug / kg+DOM 5-10mg / kg, the dosage of male fish is LRH-A2 1-3ug / kg+DOM 2-5mg / kg, the effect time of induced spawning agent is 15-19h, the flow water hatching temperature is 17-19℃, and the duration is 42-45h.The method is simple in operation, low in cost, high in induced spawning rate, fertilization rate and hatching rate, can provide technical support for large-scale breeding of Leucoglanis guichenoti, promote the development of aquaculture industry, meet market demand, and has economic and social benefits.

Description

Technical Field

[0001] This invention relates to the fields of fish farming, genetics and breeding technology, and specifically to an artificial breeding method for the dense-striped loach. Background Technology

[0002] In their natural environment, the reproduction of the Southern Blue Loach is strictly limited by natural conditions such as aquatic environment, water temperature, and light. The Southern Blue Loach only begins to reproduce when the water temperature rises to a certain level in spring. However, natural reproduction is subject to many uncontrollable factors. For example, fluctuations in water temperature may cause inconsistent mating behavior in parent fish, affecting spawning and fertilization; changes in the environment such as rainfall and sediment can damage spawning sites and reduce the hatching rate of eggs; and the presence of natural predators can also threaten the survival of eggs and fry. These natural factors have led to a decline in the wild population of the Southern Blue Loach, making it difficult to meet market demand.

[0003] In terms of artificial breeding, current practices are limited to the cultivation of similar fish species, and no artificial breeding techniques for *Sinocyclocheilus davidii* have been published. Traditional breeding methods largely rely on catching broodstock from the wild, followed by natural hatching and extensive farming. This approach not only damages wild resources but also has low reproductive efficiency, making large-scale cultivation of *Sinocyclocheilus davidii* difficult. Furthermore, due to insufficient research on the reproductive physiology of *Sinocyclocheilus davidii*, existing artificial breeding techniques have many problems regarding spawning agent formulation, application methods, and incubation environment control. For example, improper composition and dosage of spawning agents can lead to poor estrus and spawning effects in broodstock; inadequate control of water temperature and flow conditions in the incubation environment can affect the hatching rate of eggs and the survival rate of fry; and an improper ratio of male to female spawning agents will ultimately result in a significant reduction in fertilization and hatching rates.

[0004] As a fish with significant economic and ecological value, the natural reproduction of the dense-striped loach is affected by a variety of factors, and its reproductive success rate is relatively low in artificial breeding environments, which seriously limits the large-scale development of the dense-striped loach farming industry.

[0005] With the increasing demand for aquatic products and the growing awareness of ecological protection, the development of an efficient artificial breeding method for the dense-striped loach is of great significance for protecting wild resources, meeting market demand, and promoting the sustainable development of aquaculture. Summary of the Invention

[0006] This invention aims to provide an innovative artificial breeding method for the dense-striped loach, while solving the problems of low artificial breeding efficiency, low spawning rate, low fertilization rate and low hatching rate of loach in the existing technology.

[0007] The technical solution adopted in this invention is:

[0008] Firstly, a method for the artificial breeding of the dense-striped loach includes the following steps:

[0009] A spawning induced agent was used on the broodstock of the dense-striped loach, and the water temperature for using the spawning induced agent was 13-19℃.

[0010] After being treated with spawning induced agents, the parent fish of the dense-striped loach were placed in a spawning and incubation box for natural spawning and fertilization.

[0011] After the parent fish of the dense-striped loach are removed from the spawning and hatching frame, the water level in the spawning and hatching frame is maintained at 5-15cm, and the fish are hatched by flowing water with an exchange rate of 2-4 times per day to obtain hatched fry.

[0012] The hatched fish fry were cultured for more than 24 hours to obtain flounder fry.

[0013] The oxytocin includes LRH-A2 and DOM.

[0014] For female parent fish of the dense-striped loach, the dosage of the spawning induced agent is LRH-A 3~5μg / kg + DOM 5~10mg / kg.

[0015] For male broodstock of the dense-striped loach, the dosage of the spawning induced agent is LRH-A 1~3μg / kg + DOM 2~5mg / kg.

[0016] The oxytocin has an effect duration of 15–19 hours.

[0017] The temperature for the water incubation is 17–19°C.

[0018] The incubation time in the flowing water is 42 to 45 hours.

[0019] In a second aspect, the present invention provides an incubation device that provides the aforementioned artificial breeding method. The device includes a heating pool, a spawning pool, an incubation pool, a water filtration device, and a water pump forming a water circulation system. A mesh screen is provided in the spawning pool and the incubation pool. The mesh diameter is smaller than the size of the fish fry and fish eggs. A flow meter and a plug are also provided in the pipeline.

[0020] Compared with the prior art, the beneficial effects of the present invention are:

[0021] The artificial breeding method provided by this invention has the advantages of simple operation and low cost. At the same time, it has high spawning rate, fertilization rate and hatching rate, which can effectively improve the artificial breeding efficiency of loach and provide technical support for the large-scale breeding of loach.

[0022] This invention improves the spawning rate, fertilization rate and hatching rate of the artificial breeding of *Sinocyclocheilus davidii* by rationally selecting the components and dosage of spawning accelerators and optimizing spawning and hatching conditions. Attached Figure Description

[0023] Figure 1The present invention provides a structural diagram of the incubation equipment;

[0024] The components include: 1. Heating tank; 2. Spawning tank; 3. Hatching tank; 4. Water filtration equipment; 5. Water pump; 6. Mesh support.

[0025] Figure 2 A diagram showing the usage state of the mesh support provided by the present invention. Detailed Implementation

[0026] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example 1

[0027] 1. Selection and rearing of broodstock: Select robust, disease-free, and uninjured broodstock of the dense-striped loach, and pair them at a female-to-male ratio of 1:3. Place the broodstock in a rearing pond that simulates a natural ecological environment. Provide suitable roof tiles as shelter in the pond to offer a good habitat for the broodstock. During the rearing process, maintain the water temperature within the range of 13–19℃ and regularly feed them diets rich in protein, vitamins, and other nutrients to promote gonadal development.

[0028] 2. Preparation and injection of oxytocin: Prepare the oxytocin agent according to the ratio of LRH-A2 and DOM. For female broodstock, use a dosage of 3 μg / kg LRH-A2 + 5 mg / kg DOM; for male broodstock, use a dosage of 1 μg / kg LRH-A2 + 2 mg / kg DOM. Inject the oxytocin agent intramuscularly near the base of the pectoral fins of both male and female broodstock.

[0029] 3. Setting up the spawning and hatching frame: After being injected with the spawning stimulant, the parent fish of the dense-striped loach are placed in the spawning and hatching frame. The frame is made of wire mesh with a layer of fine gauze inside to facilitate the collection of fertilized eggs later. Maintain the water temperature within the range of 13-19℃ and provide a micro-flowing water environment to promote estrus, spawning, and fertilization of the parent fish.

[0030] 4. Hatching and Fry Rearing: Typically, 15-19 hours after the injection of the spawning stimulant, the broodstock complete spawning and fertilization. The broodstock are then removed from the spawning and hatching frames and returned to the outdoor broodstock pond. The fertilized eggs are then transferred from the hatching frames to the hatching pond, with the water level adjusted to 5-15 cm and the water temperature maintained between 17-19℃. A flow-through hatching process is used, with an exchange rate of 2-4 times the eggs per day or more. After 42-45 hours of incubation, the eggs hatch, yielding hatched fry. The hatched fry are then raised for at least 24 hours until they begin to swim and feed. At this point, routine fry rearing procedures can be performed, feeding appropriate amounts of starter feed to gradually improve the survival rate and growth rate of the fry. Example 2

[0031] Based on Example 1, the dosage of the spawning induced agent was adjusted. For female broodstock, a dosage of 5 μg / kg LRH-A2 + 10 mg / kg DOM was used; for male broodstock, a dosage of 3 μg / kg LRH-A2 + 5 mg / kg DOM was used. Other operating procedures were the same as in Example 1. By increasing the dosage of the spawning induced agent, the spawning effect of the broodstock was further improved, resulting in higher fertilization and hatching rates. Example 3

[0032] Based on Example 1, the incubation conditions were optimized by controlling the water temperature for flowing water incubation at 18°C ​​and increasing the water exchange rate to 3 times per day. Other operating steps remained the same as in Example 1. By precisely controlling the incubation water temperature and increasing the water exchange rate, more suitable environmental conditions were provided for fish egg incubation, promoting normal egg development and hatching, and improving the quality and quantity of hatched fish fry.

[0033] As can be seen from the above embodiments, the artificial breeding method for *Sinocyclocheilus fasciatus* provided by the present invention has high feasibility and effectiveness. In actual production, parameters such as the dosage of spawning stimulant and hatching conditions can be appropriately adjusted and optimized according to different breeding conditions and needs to achieve the best breeding effect. Example 4

[0034] This embodiment provides an incubation device for an artificial breeding method, including a heating tank (1), a spawning tank (2), an incubation tank (3), a water filtration device (4), and a water pump (5) forming a water circulation system. Spawning tank (2) and incubation tank (3) are equipped with mesh screens, the mesh diameter of which is smaller than the size of the fish fry and eggs. A flow meter (7) and a plug (8) are also installed in the pipeline. Intelligent control can be added to the entire water system according to site requirements, and software control enables refined aquaculture.

[0035] The above-described embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application, and should all be included within the protection scope of this invention.

Claims

1. A method for artificial propagation of Leptobramis modestus, characterized by, Includes the following steps: A spawning induced agent was used on the broodstock of the dense-striped loach, and the water temperature for using the spawning induced agent was 13–19°C. After being treated with spawning induced agents, the parent fish of the dense-striped loach were placed in a spawning and incubation box for natural spawning and fertilization. After removing the parent fish of the dense-striped loach from the spawning and hatching frame, the fertilized eggs are transferred to the hatching pond, where the water level is maintained at 5-15cm, and the fish are hatched by flowing water with an exchange rate of 2-4 times per day to obtain hatching fry. The hatched fish fry were cultured for more than 24 hours to obtain flounder fry.

2. The artificial breeding method for *Sinocyclocheilus davidii* according to claim 1, characterized in that, The oxytocin includes LRH-A2 and DOM.

3. The artificial breeding method for *Leptobrachium chinense* according to claim 2, characterized in that, For female parent fish of the dense-striped loach, the dosage of the spawning induced agent is LRH-A 3~5μg / kg + DOM 5~10mg / kg.

4. The artificial breeding method for *Leptobrachium chinense* according to claim 2, characterized in that, For male broodstock of the dense-striped loach, the dosage of the spawning induced agent is LRH-A 1~3μg / kg + DOM 2~5mg / kg.

5. The artificial breeding method for *Sinocyclocheilus davidii* according to claim 1, characterized in that, The oxytocin has an effect duration of 15–19 hours.

6. The artificial breeding method for *Leptobrachium chinense* according to claim 1, characterized in that, The temperature for the water incubation is 17–19°C.

7. The artificial breeding method for *Sinocyclocheilus davidii* according to claim 1, characterized in that, The incubation time in the flowing water is 42 to 45 hours.

8. An incubation device for an artificial breeding method as described in any one of claims 1-7, characterized in that, The system includes a heating pool (1), a spawning pool (2), a hatching pool (3), a water filtration device (4), and a water pump (5) to form a water circulation system. The spawning pool (2) and the hatching pool (3) are equipped with mesh nets with a mesh diameter smaller than that of the fry and eggs. The pipeline is also equipped with a flow meter (7) and a plug (8).

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