A sunscreen composition and a method of making the same

Abstract

The application discloses a sunscreen composition and a preparation method thereof, and a cosmetic containing the sunscreen composition. The sunscreen composition comprises an inula japonica extract, an actinidia deliciosa extract and an artemisia annua extract. The application further discloses a preparation method of the sunscreen composition and a sunscreen stick containing the sunscreen composition. The inula japonica extract, the actinidia deliciosa extract and the artemisia annua extract are compounded, and a significant synergistic sunscreen and antioxidant effect is generated among the three. Animal experiments further prove that the sunscreen stick containing the composition can effectively reduce ultraviolet-induced skin damage, significantly improve the SOD and CAT activities in skin tissue and reduce the MDA content. The sunscreen composition has excellent sunscreen performance and antioxidant capacity, and can be widely applied to cosmetics such as sunscreen sticks and sunscreen creams.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a sunscreen composition comprising Inula japonica extract, kiwi fruit extract and Artemisia annua extract, a method for preparing the same, and a sunscreen stick comprising the sunscreen composition. Background Technology

[0002] With the depletion of the ozone layer and increasing environmental pollution, the damage to the skin caused by ultraviolet radiation is receiving growing attention. Sunscreen cosmetics have become an essential part of people's daily skincare routine. Existing sunscreens are mainly divided into chemical sunscreens (such as ethylhexyl methoxycinnamate and bis-ethylhexyloxyphenol methoxyphenyl triazine) and physical sunscreens (such as titanium dioxide and zinc oxide). However, chemical sunscreens have potential problems such as skin irritation and poor photostability, while physical sunscreens are prone to whitening and a heavy feel on the skin. Therefore, developing safe, gentle, and synergistically effective natural plant-derived sunscreen ingredients has become a hot research topic in the industry.

[0003] Chinese patent CN112156035A discloses a sunscreen stick and its preparation method. This sunscreen stick is a pure oil-based formula, free of water and emulsifiers. By selecting liquid oils with specific kinematic viscosities and thickeners, it solves the problems of greasiness and makeup-damaging effects found in commercially available sunscreen sticks. However, this patent does not involve the application of plant extracts as active sunscreen ingredients, nor does it suggest the synergistic effect of combining multiple plant extracts.

[0004] Inula japonica Thunb., a plant belonging to the genus Inula in the family Asteraceae, mainly contains active ingredients such as flavonoids, sesquiterpene lactones, and polysaccharides. Existing research indicates that Inula japonica extract possesses skin-care effects such as antioxidant, anti-inflammatory, and whitening properties. Literature reports that total flavonoids from Inula japonica exhibit good DPPH free radical scavenging ability, and its 70% ethanol extract shows significantly stronger ABTS free radical scavenging activity than its water extract, indicating the potential of Inula japonica extract in scavenging free radicals. Furthermore, studies have combined Inula japonica extract with other plant extracts in sunscreen products, demonstrating that synergistic formulation can increase the UVA and UVB index of sunscreens and significantly enhance the sun protection effect of the composition. However, current applications of Inula japonica extract in the cosmetic field mainly focus on anti-aging, whitening, and anti-inflammatory effects; research on its application in sunscreen is still limited, and there are no reports of combining it with kiwi fruit extract and artemisia annua extract for sunscreen applications.

[0005] Kiwifruit (Actinidia chinensis Planch.), also known as Chinese kiwifruit, is rich in antioxidants such as polyphenols, flavonoids, polysaccharides, and vitamin C. Studies have shown that kiwifruit polyphenols have excellent scavenging abilities against hydroxyl radicals, superoxide radicals, DPPH radicals, and ABTS radicals. Its total reducing power is higher than that of vitamin C, and its IC50 for scavenging these four free radicals is significantly higher. 50 The values ​​were all lower than those of vitamin C, indicating high antioxidant activity. Recent studies have shown that applying ethanol extract of *Actinidia chinensis* to the backs of hairless mice effectively improved UVB-mediated wrinkle formation, epidermal thickening, and collagen loss, and could effectively prevent photoaging by inhibiting the production of reactive oxygen species. Furthermore, kiwifruit extract has been used in cosmetics for whitening, moisturizing, and anti-aging effects, but its application in sun protection remains relatively limited.

[0006] Artemisia annua L. is an annual herb belonging to the genus Artemisia in the family Asteraceae. Artemisia annua volatile oil is obtained through further purification of the residues from the artemisinin extraction process. Recent studies have revealed its broad application prospects in the cosmetics field. Research indicates that Artemisia annua oil (AN+) possesses excellent anti-inflammatory, soothing, microecological regulation, and skin barrier repair effects, making it highly suitable for sensitive skin. Other studies, through the determination of matrix metalloproteinase 1 and reactive oxygen species content in irradiated skin models, have confirmed that Artemisia annua volatile oil has good antioxidant and anti-wrinkle effects. Furthermore, compositions combining Artemisia annua extract and Artemisia annua oil exhibit anti-inflammatory, antioxidant, repairing, and nourishing effects, making them suitable for sensitive skin care. However, current applications of Artemisia annua extract in cosmetics primarily focus on anti-inflammatory, soothing, and repairing properties; research on its combination with Inula japonica and Actinidia chinensis extracts for use in sunscreen compositions has not yet been reported.

[0007] In summary, Inula japonica extract, kiwifruit extract, and Artemisia annua extract each possess certain antioxidant activity and skincare benefits. However, there are no reports in the current technology of combining these three extracts in sunscreen products to achieve synergistic effects. Developing a natural plant extract composition that combines synergistic sun protection and antioxidant effects, and applying it to products such as sunscreen sticks, has significant practical implications and market value. Summary of the Invention

[0008] This invention aims to provide a sunscreen composition with synergistic sun protection and antioxidant effects, and its preparation method, to address the problems of insufficient sun protection effect of single plant extracts and questionable safety of chemical sunscreens in existing sunscreen products. This invention also provides a sunscreen stick containing this sunscreen composition, which, in addition to good sun protection performance, also possesses multiple benefits such as antioxidant, soothing, and repairing properties.

[0009] To achieve the above objectives, the present invention provides the following technical solution: A sunscreen composition comprising Inula japonica extract, kiwifruit extract and Artemisia annua extract.

[0010] Furthermore, the mass ratio of the Inula japonica extract, kiwifruit extract and Artemisia annua extract is 1-5:1-5:1-5, preferably 1-2:1-2:1-2, and even more preferably 1:1:1.

[0011] The present invention also provides a method for preparing the sunscreen composition, comprising: reflux extraction of Inula japonica, Actinidia chinensis and Artemisia annua with 60-70% ethanol solution, concentration of the extract under reduced pressure to obtain extracts, drying the three extracts and sieving them, and mixing them evenly in proportion to obtain the sunscreen composition.

[0012] Specifically, the preparation method includes: Preparation of Inula japonica extract: Take Inula japonica, add 60-70% ethanol solution at a material-to-liquid ratio of 1:10-20, heat and reflux to extract 2-3 times, 1-2 hours each time, combine the filtrates, concentrate under reduced pressure at 50-60℃ until there is no alcohol odor, and obtain Inula japonica extract paste.

[0013] Preparation of kiwifruit extract: Take kiwifruit, add 60-70% ethanol solution at a material-to-liquid ratio of 1:15-25, heat and reflux to extract 2-3 times, 1-2 hours each time, combine the filtrates, and concentrate under reduced pressure at 50-60℃ until there is no alcohol taste to obtain kiwifruit extract paste.

[0014] Preparation of Artemisia annua extract: Artemisia annua was taken and 60-70% ethanol solution was added at a material-to-liquid ratio of 1:10-20. The mixture was heated and refluxed for extraction 2-3 times, each time for 1-2 hours. The filtrates were combined and concentrated under reduced pressure at 50-60℃ until no alcohol odor was detected, thus obtaining Artemisia annua extract paste.

[0015] The above three extracts were vacuum dried to constant weight, pulverized through a 100-mesh sieve, and mixed evenly in proportion to obtain the final product.

[0016] The present invention also provides a cosmetic comprising the above-described sun protection composition, wherein the cosmetic is a sun protection stick, sun protection cream, sun protection lotion, sun protection gel, sun protection jelly, sun protection oil, or sun protection base makeup, preferably a sun protection stick.

[0017] The sunscreen stick also includes the following components: 10-25 parts of decanoic acid triglycerides 5-15 parts of ethylhexyl palmitate 15-25 parts of polydimethylsiloxane 5-10 parts of isopropyl myristate 5-15 parts of carnauba wax 5-10 parts of microcrystalline wax 2-5 parts of ethylhexyl methoxycinnamate 2-5 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine 2-5 parts of ethylhexyl salicylate 5-10 parts of silica 0.2-0.8 parts of bisabolol Tocopherol acetate 0.2~0.8 parts.

[0018] The preparation method of the sunscreen stick includes the following steps: 1) Mix decanoic acid triglyceride, ethylhexyl palmitate, polydimethylsiloxane, isopropyl myristate, carnauba wax, and microcrystalline wax, heat to 85~95℃, and stir continuously until completely melted and evenly mixed.

[0019] 2) Add ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl salicylate, and silica to the molten mixture and homogenize until evenly dispersed.

[0020] 3) After the mixture cools to 75~80℃, add the above sunscreen composition, bisabolol and tocopheryl acetate, and stir quickly until homogeneous.

[0021] 4) Pour the molten mixture into a rod-shaped mold, cool to room temperature to allow the wax base to solidify and solidify, and then demold to obtain the sunscreen stick.

[0022] Compared with the prior art, the present invention has the following beneficial effects: The combination of three plant extracts produced significant synergistic sun protection and antioxidant effects. In vitro SPF testing showed that, under the condition of the same total active ingredient dosage, the SPF value of the combination of the three extracts in equal mass ratios was significantly higher than that of any single extract, and the dosage of each extract in the composition was only 1 / 3 of that of the single extract, achieving a synergistic effect of "reduced dosage, increased efficacy". DPPH and ABTS free radical scavenging experiments showed that the scavenging rate of the composition was significantly higher than that of the individual extracts, providing intrinsic mechanistic support for the sun protection effect.

[0023] Animal experiments have confirmed its excellent UV protection effect. Sunscreen sticks containing the sunscreen composition of this invention can significantly reduce UV-induced skin damage in mice, increase SOD and CAT activity, and reduce MDA content, showing better effects than sunscreen sticks containing only chemical sunscreens. Detailed Implementation

[0024] The embodiments of the present invention will be clearly and completely described below with reference to the examples. These embodiments are merely some, not all, of the embodiments of the present invention. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the present invention or its application or use. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention. Those skilled in the art should understand that modifications or substitutions to the details and form of the technical solutions of this application can be made without departing from the spirit and scope of this application, but such modifications and substitutions all fall within the scope of protection of this application.

[0025] Furthermore, to better illustrate the present invention, numerous specific details are set forth in the following detailed embodiments. Those skilled in the art should understand that the present invention can be practiced without certain specific details. In some embodiments, materials, methods, and means well-known to those skilled in the art are not described in detail in order to highlight the spirit of the invention.

[0026] Unless otherwise specified, the instruments and materials used in the following embodiments are all commonly used laboratory equipment, and the technical solutions described are all conventional technologies in the field.

[0027] Example 1 A sunscreen composition, the preparation method of which includes the following steps: (1) Preparation of Inula japonica extract: Take the dried flower head of Inula japonica, remove impurities, dry and crush and sieve to obtain Inula japonica pollen. Add 70% ethanol solution at a material-to-liquid ratio of 1:10, heat and reflux to extract twice, each time for 1.5 hours. Combine the two filtrates and concentrate under reduced pressure at 50°C until there is no alcohol taste to obtain Inula japonica extract paste.

[0028] (2) Preparation of kiwifruit extract: Take fresh kiwifruit, remove hairs and wash, cut into thin slices or small pieces, dry and crush and sieve to obtain kiwifruit powder. Add 65% ethanol solution at a material-to-liquid ratio of 1:20, heat and reflux to extract twice, each time for 2 hours, combine the two filtrates, and concentrate under reduced pressure at 50℃ until there is no alcohol taste to obtain kiwifruit extract paste.

[0029] (3) Preparation of Artemisia annua extract: Take the dried aerial parts of Artemisia annua, remove the coarse stems, crush and sieve to obtain Artemisia annua powder, add 70% ethanol solution at a material-to-liquid ratio of 1:10, heat and reflux to extract twice, each time for 1.5 hours, combine the two filtrates, and concentrate under reduced pressure at 50°C until there is no alcohol taste to obtain Artemisia annua extract paste.

[0030] The above three extracts were vacuum dried to constant weight, pulverized and passed through a 100-mesh sieve, and then mixed evenly in a mass ratio of 1:1:1 to obtain the sunscreen composition.

[0031] Example 2 A sunscreen composition, the preparation method of which includes the following steps: (1) Preparation of Inula japonica extract: Take the dried flower head of Inula japonica, remove impurities, dry and crush and sieve to obtain Inula japonica pollen. Add 65% ethanol solution at a material-to-liquid ratio of 1:15, heat and reflux twice for 2 hours each time, combine the two filtrates, and concentrate under reduced pressure at 60°C until there is no alcohol taste to obtain Inula japonica extract paste.

[0032] (2) Preparation of kiwifruit extract: Take fresh kiwifruit, remove hairs and wash, cut into thin slices or small pieces, dry and crush and sieve to obtain kiwifruit powder. Add 70% ethanol solution at a material-to-liquid ratio of 1:15, heat and reflux twice for 1.5 hours each time, combine the two filtrates, and concentrate under reduced pressure at 55℃ until there is no alcohol taste to obtain kiwifruit extract paste.

[0033] (3) Preparation of Artemisia annua extract: Take the dried aerial parts of Artemisia annua, remove the coarse stems, crush and sieve to obtain Artemisia annua powder, add 65% ethanol solution at a material-to-liquid ratio of 1:15, heat and reflux to extract twice, each time for 2 hours, combine the two filtrates, and concentrate under reduced pressure at 60℃ until there is no alcohol taste to obtain Artemisia annua extract paste.

[0034] The above three extracts were vacuum dried to constant weight, pulverized and passed through a 100-mesh sieve, and then uniformly mixed in a mass ratio of 2:1:1 to obtain the sunscreen composition.

[0035] Example 3 A sunscreen composition, the preparation method of which includes the following steps: (1) Preparation of Inula japonica extract: Take the dried flower head of Inula japonica, remove impurities, dry and crush and sieve to obtain Inula japonica pollen. Add 60% ethanol solution at a material-to-liquid ratio of 1:10, heat and reflux twice for 2 hours each time, combine the two filtrates, and concentrate under reduced pressure at 55℃ until there is no alcohol taste to obtain Inula japonica extract paste.

[0036] (2) Preparation of kiwifruit extract: Take fresh kiwifruit, remove hairs and wash, cut into thin slices or small pieces, dry and crush and sieve to obtain kiwifruit powder. Add 60% ethanol solution at a material-to-liquid ratio of 1:25, heat and reflux twice for 2 hours each time, combine the two filtrates, and concentrate under reduced pressure at 60℃ until there is no alcohol taste to obtain kiwifruit extract paste.

[0037] (3) Preparation of Artemisia annua extract: Take the dried aerial parts of Artemisia annua, remove the coarse stems, crush and sieve to obtain Artemisia annua powder, add 70% ethanol solution at a material-to-liquid ratio of 1:20, heat and reflux to extract twice, each time for 1.5 hours, combine the two filtrates, and concentrate under reduced pressure at 50°C until there is no alcohol taste to obtain Artemisia annua extract paste.

[0038] The above three extracts were vacuum dried to constant weight, pulverized and passed through a 100-mesh sieve, and then uniformly mixed in a mass ratio of 2:2:1 to obtain the sunscreen composition.

[0039] Example 4 A sunscreen stick, comprising the following components by weight: The preparation method of the sunscreen stick is as follows: 1) Mix decanoic acid triglyceride, ethylhexyl palmitate, polydimethylsiloxane, isopropyl myristate, carnauba wax, and microcrystalline wax, heat to 90°C, and stir continuously until completely melted and evenly mixed.

[0040] 2) Add ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl salicylate, and silica to the molten mixture and homogenize until evenly dispersed.

[0041] 3) After the mixture has cooled to 75°C, add the sunscreen composition prepared in Example 1, bisabolol and tocopheryl acetate, and stir quickly until homogeneous.

[0042] 4) Pour the molten mixture into a rod-shaped mold, cool to room temperature to allow the wax base to solidify and solidify, and then demold to obtain the sunscreen stick.

[0043] Example 5 A sunscreen stick, comprising the following components by weight: The preparation method of the sunscreen stick is as follows: 1) Mix decanoic acid triglyceride, ethylhexyl palmitate, polydimethylsiloxane, isopropyl myristate, carnauba wax, and microcrystalline wax, heat to 85°C, and stir continuously until completely melted and evenly mixed.

[0044] 2) Add ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl salicylate, and silica to the molten mixture and homogenize until evenly dispersed.

[0045] 3) After the mixture has cooled to 75°C, add the sunscreen composition prepared in Example 2, bisabolol and tocopheryl acetate, and stir quickly until homogeneous.

[0046] 4) Pour the molten mixture into a rod-shaped mold, cool to room temperature to allow the wax base to solidify and solidify, and then demold to obtain the sunscreen stick.

[0047] Example 6 A sunscreen stick, comprising the following components by weight: The preparation method of the sunscreen stick is as follows: 1) Mix decanoic acid triglyceride, ethylhexyl palmitate, polydimethylsiloxane, isopropyl myristate, carnauba wax, and microcrystalline wax, heat to 95°C, and stir continuously until completely melted and evenly mixed.

[0048] 2) Add ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl salicylate, and silica to the molten mixture and homogenize until evenly dispersed.

[0049] 3) After the mixture cools to 80°C, add the sunscreen composition prepared in Example 3, bisabolol and tocopheryl acetate, and stir quickly until homogeneous.

[0050] 4) Pour the molten mixture into a rod-shaped mold, cool to room temperature to allow the wax base to solidify and solidify, and then demold to obtain the sunscreen stick.

[0051] Comparative Example A sunscreen stick, comprising the following components by weight: The preparation method of the sunscreen stick is as follows: 1) Mix decanoic acid triglyceride, ethylhexyl palmitate, polydimethylsiloxane, isopropyl myristate, carnauba wax, and microcrystalline wax, heat to 90°C, and stir continuously until completely melted and evenly mixed.

[0052] 2) Add ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl salicylate, and silica to the molten mixture and homogenize until evenly dispersed.

[0053] 3) After the mixture cools to 75°C, add bisabolol and tocopheryl acetate, and stir quickly until homogeneous.

[0054] 4) Pour the molten mixture into a rod-shaped mold, cool to room temperature to allow the wax base to solidify and solidify, and then demold to obtain the sunscreen stick.

[0055] Experimental Example 1 Sunscreen performance test 1) Sample preparation: The sunscreen composition prepared in Example 1 was mixed with liquid paraffin in proportion to form a 50 wt% uniform dispersion (Sample 1) without clumping. The same method was used to prepare 50 wt% uniform dispersions of the sunscreen composition of Example 2 and the sunscreen composition of Example 3 (Sample 2 and Sample 3). In addition, the dried and sieved Inula japonica extract, kiwi fruit extract and Artemisia annua extract in Example 1 were prepared into 50 wt% uniform dispersions (Sample 4, Sample 5 and Sample 6) using the same method.

[0056] 2) Application and measurement: Apply samples 1-6 at 1.3 mg / cm³. 2 The material was evenly applied to a 5cm × 5cm PMMA surface to form a uniform film. After application, the PMMA plate was left to stand at room temperature in the dark for 30 minutes. Using a UV-2000S ultraviolet transmittance analyzer, at least five different points on the plate were scanned within the wavelength range of 290-400nm, and the absorbance values ​​were recorded. The SPF value was then calculated. The results are shown in Table 1. Table 1 SPF values ​​of each sample ( ±s ) Note: Compared with sample 4, a P <0.05; compared with sample 5, b P <0.05, c P <0.01; compared with sample 6, d P <0.05, e P <0.01 Table 1 shows that all three plant extracts, when used alone, have sun protection effects (SPF 4.35~7.38). When combined in equal mass ratios (Sample 1) with the same total active ingredient dosage, the SPF value reached 13.09, significantly higher than any single extract (P<0.05 or 0.01), indicating that the combination of the three extracts produced a synergistic effect, significantly enhancing sun protection capabilities.

[0057] Experimental Example 2 Antioxidant performance test 1) Sample preparation: The sunscreen composition prepared in Example 1 was dissolved in anhydrous ethanol to prepare a test sample (sample 1) with a concentration of 2 mg / mL; the sunscreen compositions of Example 2 and Example 3 were prepared in the same way to obtain test samples (sample 2 and sample 3) with a concentration of 2 mg / mL; in addition, the dried and sieved Inula japonica extract, kiwi fruit extract and Artemisia annua extract of Example 1 were prepared in the same way to obtain test samples (sample 4, sample 5 and sample 6) with a concentration of 2 mg / mL.

[0058] 2) DPPH free radical scavenging assay: Weigh DPPH powder, dissolve and dilute it to a concentration of 0.1 mM with anhydrous ethanol. Add 100 μL of DPPH solution and 100 μL of 2 mg / mL sample solution to each well of a 96-well plate, and mix well. Incubate at room temperature in the dark for 30 minutes to ensure complete reaction. Measure the absorbance of each reaction system at 517 nm using a microplate reader. Simultaneously measure the absorbance of the control group (using solvent instead of sample solution) and the blank group (using solvent instead of DPPH solution). Calculate the DPPH scavenging rate using the following formula: DPPH clearance rate = [1 - (sample group - blank group) / control group] × 100% 3) ABTS free radical scavenging experiment: Mix 7 mM ABTS and 2.45 mM potassium persulfate in equal volumes, and let stand overnight at room temperature in the dark to allow for complete reaction and formation of stable ABTS. + • Free radicals. Dilute the ABTS stock solution with anhydrous ethanol to achieve an absorbance of 0.70 ± 0.02 at 734 nm. Add 200 μL of ABTS working solution and 20 μL of 2 mg / mL sample solution to a 96-well plate and mix well. Incubate at room temperature in the dark for 6–10 minutes to ensure complete reaction. Measure the absorbance of each reaction system at 734 nm using a microplate reader. Simultaneously measure the absorbance of the control group (using solvent instead of sample solution) and the blank group (using solvent instead of ABTS working solution). The scavenging rate is calculated using the same formula as the DPPH method.

[0059] The DPPH and ABTS clearance rates are shown in Table 2 below: Table 2. Scavenging rate (%) of each sample against DPPH / ABTS free radicals ±s ) Note: Compared with sample 4, a P <0.05; compared with sample 5, b P <0.05, c P <0.01; compared with sample 6, d P <0.05, e P <0.01 Table 2 shows that each of the three plant extracts, when used alone, possessed antioxidant activity (DPPH scavenging rate 43.85%–52.46%, ABTS scavenging rate 36.72%–42.55%). When combined in equal mass ratios (Sample 1) at the same total concentration, the DPPH scavenging rate reached 78.52%, and the ABTS scavenging rate reached 63.41%, both significantly higher than any single extract (P<0.05 or 0.01), indicating that the combination of the three extracts has a synergistic antioxidant effect.

[0060] Experimental Example 3 Protective effect of sunscreen sticks against UV radiation-induced skin damage in mice Given that Inula japonica extract, kiwi fruit extract, and Artemisia annua extract have synergistic sun protection and antioxidant effects, a sunscreen composition containing the above three extracts was made into a sunscreen stick, and the sun protection effect of the sunscreen stick was further verified through animal experiments.

[0061] Experimental Methods: Sixty healthy Kunming mice were randomly divided into six groups: normal group, model group, Examples 4-6, and comparative group, with 10 mice in each group. Long hair on the backs of the mice was shaved using a shaver, and the hair was removed using 8% sodium sulfide. The hair removal area was approximately 3cm × 3cm. Except for the normal group, a UV damage model was established in all other groups. A UVB lamp was fixed 15cm away from the exposed skin on the back of the mice, with an irradiation intensity of 120 μW / cm². 2 The sunscreen was applied twice a week for four consecutive weeks, with each application lasting 30 minutes. For groups 4-6 and the comparative group, the appropriate sunscreen stick was applied evenly 30 minutes before each application, at a concentration of 2 mg / cm³. 2 .

[0062] Detection indicators: Visual observation of skin damage in each group; detection of SOD, CAT activity and MDA content in skin tissue using reagent kits.

[0063] Experimental results 1. Observation results of the appearance of the skin on the back of mice The skin on the backs of mice in the normal group was smooth, delicate, and of normal color, with good elasticity and no erythema, wrinkles, or desquamation. The skin on the backs of mice in the model group was significantly rough and thickened, with deep and coarse wrinkles, localized redness, ulceration, and numerous scabs; the skin was leathery and lacked elasticity. The sunscreen sticks in the control group contained only chemical sunscreen agents; the overall smoothness of the back skin of the mice in this group was significantly improved, with shallower and fewer wrinkles, no obvious redness or ulceration, only a few small scabs, and good skin elasticity; the appearance was significantly better than the model group. The back skin of mice in Examples 4-6 was smooth and delicate, with almost no visible wrinkles, normal color, no erythema, scabs, or desquamation, and good skin elasticity; the overall appearance was better than the control group and approached the level of the normal group. Among these, Example 4 showed the best protective effect, slightly better than Examples 5 and 6.

[0064] The results above indicate that the sunscreen sticks containing three plant extracts (Examples 4-6) have a significant protective effect against UV-induced skin damage, and are all superior to the comparative examples containing only chemical sunscreens.

[0065] 2. SOD and CAT activities and MDA content in the skin tissue of mice in each group The results are shown in Table 3 below. The SOD and CAT activities in the skin of mice in the model group were significantly reduced, while the MDA content was significantly increased (P<0.01 compared to the normal group), indicating that ultraviolet radiation induced severe oxidative stress damage. Compared to the model group, all treatment groups significantly improved the above indicators (P<0.01). Among them, the SOD and CAT activities in Examples 4-6 were higher than those in the comparative group, while the MDA content was lower, indicating that the sunscreen stick containing the three plant extracts had a better antioxidant effect than the comparative group containing only chemical sunscreen. Example 4 showed the highest SOD activity (51.27 U / mgprot) and CAT activity (13.46 U / mgprot), and the lowest MDA content (4.53 nmol / mgprot), with significant differences compared to the comparative group (P<0.05), showing the best effect among the three examples. Examples 5 and 6 also showed better antioxidant activity than the comparative group. These results further confirm the synergistic effect of the plant extract combination in enhancing the antioxidant protection of the sunscreen stick.

[0066] Table 3. SOD and CAT activities and MDA content in the skin tissue of mice in each group ( ±s ) Note: Compared with the normal group, a P <0.01; compared with the model group, b P <0.01; compared with the comparative example, c P <0.05 Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

[0067] As used in this invention, the terms "comprising" and "including" are open-ended expressions, meaning they include the contents specified in this invention but do not exclude other aspects.

[0068] As used in this invention, the term "and / or" includes any one or more of the related listed items and all combinations thereof.

[0069] The scope of protection of this invention is not limited to the above embodiments. Any variations and advantages that can be conceived by those skilled in the art without departing from the spirit and scope of this invention are included in this invention and are protected by the appended claims.

Claims

1. A sunscreen composition, characterized in that, The sunscreen composition includes Inula japonica extract, kiwi fruit extract, and Artemisia annua extract.

2. The sunscreen composition according to claim 1, characterized in that, The mass ratio of the Inula japonica extract, kiwifruit extract and Artemisia annua extract is 1~5:1~5:1~5, preferably 1~2:1~2:1~2, and more preferably 1:1:

1.

3. A method for preparing the sunscreen composition as described in claim 1, characterized in that, The preparation method includes the following steps: Inula japonica, Actinidia chinensis, and Artemisia annua were extracted by reflux with ethanol solution. The extracts were concentrated under reduced pressure to obtain extracts. The three extracts were dried, sieved, and mixed evenly in proportion to obtain the sunscreen composition.

4. The method for preparing the sunscreen composition according to claim 3, characterized in that, The ethanol solution is a 60-70% ethanol solution, and it is extracted by heating and reflux 1-3 times, each time for 1-2 hours.

5. The method for preparing the sunscreen composition according to claim 4, characterized in that, The preparation method includes the following steps: (1) Preparation of Inula japonica extract: Take Inula japonica, add 60-70% ethanol solution at a material-to-liquid ratio of 1:10-20, heat and reflux to extract 2-3 times, each time for 1-2 hours, combine the filtrates, and concentrate under reduced pressure at 50-60℃ until there is no alcohol taste to obtain Inula japonica extract paste. (2) Preparation of kiwifruit extract: Take kiwifruit, add 60-70% ethanol solution at a material-to-liquid ratio of 1:15-25, heat and reflux to extract 2-3 times, each time for 1-2 hours, combine the filtrates, and concentrate under reduced pressure at 50-60℃ until there is no alcohol taste to obtain kiwifruit extract paste. (3) Preparation of Artemisia annua extract: Take Artemisia annua, add 60-70% ethanol solution at a material-to-liquid ratio of 1:10-20, heat and reflux to extract 2-3 times, each time for 1-2 hours, combine the filtrates, and concentrate under reduced pressure at 50-60℃ until there is no alcohol taste to obtain Artemisia annua extract paste. The above three extracts were vacuum dried to constant weight, pulverized and passed through a 100-mesh sieve, and then uniformly mixed at a mass ratio of 1~2:1~2:1~2 to obtain the sunscreen composition.

6. A cosmetic product, characterized in that, The cosmetic product contains 1-20 parts by weight. The sunscreen composition as described in claim 1.

7. The cosmetic product as described in claim 6, characterized in that, The cosmetics include sunscreen sticks, sunscreen creams, sunscreen lotions, sunscreen gels, sunscreen jelly, sunscreen oils, and sunscreen base makeup.

8. The cosmetic product as described in claim 7, characterized in that, The cosmetic product in question is a sunscreen stick.

9. The cosmetic product as described in claim 8, characterized in that, The sunscreen stick also includes the following components: 10-25 parts of decanoic acid triglycerides 5-15 parts of ethylhexyl palmitate 15-25 parts of polydimethylsiloxane 5-10 parts of isopropyl myristate 5-15 parts of carnauba wax 5-10 parts of microcrystalline wax 2-5 parts of ethylhexyl methoxycinnamate 2-5 parts of bis-ethylhexyloxyphenol methoxyphenyl triazine 2-5 parts of ethylhexyl salicylate 5-10 parts of silica 0.2-0.8 parts of bisabolol Tocopherol acetate 0.2~0.8 parts.

10. The cosmetic product as described in claim 9, characterized in that, The preparation method of the sunscreen stick includes the following steps: 1) Mix decanoic acid triglyceride, ethylhexyl palmitate, polydimethylsiloxane, isopropyl myristate, carnauba wax, and microcrystalline wax, heat to 85~95℃, and stir continuously until completely melted and evenly mixed. 2) Add ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl salicylate, and silica to the molten mixture and homogenize until evenly dispersed. 3) After the mixture cools to 75~80℃, add the sunscreen composition according to claim 1, bisabolol and tocopheryl acetate, and stir quickly until uniform. 4) Pour the molten mixture into a rod-shaped mold, cool to room temperature to allow the wax base to solidify and solidify, and then demold to obtain the sunscreen stick.

Images