A homeothermic organism 3D printing device considering cell activity

Abstract

The present application relates to the technical field of biological 3D printing, and particularly discloses a constant-temperature biological 3D printing device considering cell activity, wherein an isolation and heat preservation module is internally installed on a printing platform, and is used for protecting a printing object; a following lifting module is fixedly connected to one side of the bottom of the isolation and heat preservation module; a temperature module is further installed in the isolation and heat preservation module, and the printing platform is fixedly connected to the outside of the temperature module; before starting a printing task, the temperature control module can pre-heat the printing area, so that the initial printing environment temperature matches the activity requirement of biological ink and cells, and the forming quality of a printed structure is guaranteed from the source; along with the step-by-step upward movement of a printing head, the following lifting module is synchronously driven to move the isolation and heat preservation module upward, so that the isolation and heat preservation module is always wrapped outside the printing object, the following heat preservation of the printing structure is realized without interruption, a relatively stable constant-temperature environment is provided, and the cell activity of the printing object is effectively maintained.

Description

Technical Field

[0001] This invention relates to the field of bio-3D printing technology, specifically to a temperature-controlled bio-3D printing device that takes into account cell activity. Background Technology

[0002] Bioprinting equipment utilizes 3D CAD model data to print biological materials and cells into three-dimensional entities through layer-by-layer printing and stacking. The core characteristic that distinguishes bioprinting technology from traditional industrial 3D printing is its use of active biological materials (such as cell-hydrogel composites and bio-inks) as the molding substrate, aiming to construct bioactive three-dimensional tissue / organ biomimetic structures. However, the bioprinting process is often time-consuming, especially when constructing large-size, high-precision, or multi-component composite active biomimetic structures, where the printing cycle can extend from several hours to tens of hours.

[0003] During this lengthy printing process, the pre-formed precursor structure is continuously exposed to an open environment, and its temperature changes uncontrollably over time. On the one hand, the heat released during the curing of the bio-ink itself (such as the polymerization heat caused by photocuring and the reaction heat generated by ionic cross-linking) will cause the temperature of local areas to rise. On the other hand, the product is in an open environment during the printing process, and as the heat diffuses and dissipates, the temperature of the pre-printed structure will gradually drop.

[0004] Such dynamic temperature fluctuations can have multi-dimensional negative impacts on the biocompatibility and functional activity of active structures:

[0005] Cell viability damage: Increased temperature can disrupt the balance of enzyme activity within cells and accelerate apoptosis; a sudden drop in temperature can damage the fluidity and integrity of the cell membrane, leading to cell inactivation and directly reducing the cell survival rate of the printed structure.

[0006] Biomaterial performance degradation: Temperature changes can alter the crosslinking density and network structure of bio-inks, causing differences in the mechanical properties (such as elastic modulus and tensile strength) of the first-printed areas compared to the later-printed areas. This results in a decrease in the mechanical uniformity of the structure and may even lead to molding defects such as interlayer delamination and cracking.

[0007] Inactivation of bioactive factors: If the bio-ink contains active substances such as growth factors and cytokines, temperature fluctuations will destroy their spatial conformation, causing them to lose their biological function, which in turn affects the subsequent cell proliferation, differentiation and tissue regeneration ability of the printed structure. Therefore, a constant temperature bio-3D printing device that takes into account cell activity is proposed to address the above problems. Summary of the Invention

[0008] The purpose of this invention is to provide a constant-temperature bio-3D printing device that takes into account cell activity, so as to solve the problems mentioned in the background art. This invention fully considers cell activity during the printing process and provides a relatively constant-temperature printing environment.

[0009] To achieve the above objectives, the present invention provides the following technical solution:

[0010] As an alternative solution to the isothermal bio-3D printing device considering cell activity described in this invention, wherein: an isothermal bio-3D printing device considering cell activity includes a horizontally movable base;

[0011] A printing platform is installed above the horizontally movable base. An isolation and heat preservation module is installed inside the printing platform to protect the printed material.

[0012] The printing platform is arranged in two layers;

[0013] A following lifting module is fixedly connected to one side of the bottom of the insulation module, and the bottom of the following lifting module is fixedly connected to the lower plate of the printing platform.

[0014] The insulation module also contains a temperature module, and the outside of the temperature module is fixedly connected to a printing platform.

[0015] As an alternative solution to the isothermal bio-3D printing device considering cell activity described in this invention, the printing platform is composed of upper and lower plates, and the upper plate of the printing platform is fixedly connected to the horizontally moving base, which is used to drive the printing platform to move horizontally.

[0016] As an optional solution of the isothermal bio-3D printing device considering cell activity described in this invention, the isolation and insulation module includes a movable frame, and a following lifting module is fixedly connected to one side of the bottom of the movable frame. The bottom of the following lifting module is fixedly connected to the lower plate of the printing platform.

[0017] An insulation layer is fixedly connected to the inside of the movable frame, and air vents are opened on the inside of both the movable frame and the insulation layer.

[0018] The interior of the moving frame is hollow, and a temperature module is installed on the inner side of the moving frame;

[0019] The movable frame extends through the upper plate of the printing platform and is slidably connected to the upper plate of the printing platform.

[0020] As an optional solution of the isothermal bio-3D printing device considering cell activity described in this invention, the following is provided: a 3D printing device is fixedly connected to the rear side of the horizontally moving base, two sets of vertical moving mechanisms are fixedly connected to the front side of the 3D printing device, and a printing nozzle is installed between the vertical moving mechanisms.

[0021] As an optional solution of the isothermal biological 3D printing device considering cell activity described in this invention, the following lifting module includes a fixed cylinder that is fixedly connected to the lower plate of the printing platform, a motor that is fixedly connected inside the fixed cylinder, a threaded shaft that is fixedly connected to the end of the motor's main shaft, a movable sleeve that is spirally connected to the outside of the threaded shaft, and a fixed connection between the top of the movable sleeve and the isolation and heat preservation module.

[0022] Bioprinting is often time-consuming, especially when constructing large-scale, high-precision, or multi-component composite biomimetic structures. Printing cycles can extend from several hours to tens of hours. During this lengthy process, the pre-printed structure is continuously exposed to an open environment, causing uncontrollable temperature changes over time. On one hand, the exothermic curing of the bio-ink itself leads to localized temperature increases; on the other hand, the open environment during printing causes the temperature of the pre-printed structure to gradually decrease as heat diffuses and dissipates. This dynamic temperature fluctuation negatively impacts the biocompatibility and functional activity of the bio-structure in multiple dimensions.

[0023] Cell viability damage: Increased temperature can disrupt the balance of enzyme activity within cells and accelerate apoptosis; a sudden drop in temperature can damage the fluidity and integrity of the cell membrane, leading to cell inactivation and directly reducing the cell survival rate of the printed structure.

[0024] Biomaterial performance degradation: Temperature changes can alter the crosslinking density and network structure of bio-inks, causing differences in the mechanical properties of the first-printed areas compared to the later-printed areas. This results in a decrease in the mechanical uniformity of the structure and may even lead to molding defects such as interlayer delamination and cracking.

[0025] Inactivation of bioactive factors: If the bio-ink contains active substances such as growth factors and cytokines, temperature fluctuations will disrupt their spatial conformation, causing them to lose their biological function, which in turn affects the subsequent cell proliferation, differentiation and tissue regeneration capabilities of the printed structure.

[0026] To address the issue of bioactivity degradation caused by temperature fluctuations in the pre-formed structure during bio-3D printing, this solution employs an integrated thermal insulation system for the printing platform. The specific technical optimizations are as follows:

[0027] Follow-up insulation design of insulation modules

[0028] An insulation module is embedded inside the printing platform. Before the printing job starts, the temperature control module preheats the printing area to ensure that the initial printing temperature matches the activity requirements of the bio-ink and cells, guaranteeing the quality of the printed structure from the outset. During printing, as the print head gradually moves upward, the linked lifting module synchronously moves the insulation module upward, ensuring that the insulation module always covers the outside of the printed object, achieving uninterrupted insulation of the printed structure throughout the entire process and effectively maintaining the bioactivity of the printed object.

[0029] As an optional solution of the isothermal biological 3D printing device considering cell activity described in this invention, the temperature module includes a movable frame disposed inside the insulation module. The movable frame has uniformly distributed through holes on its inner side. The bottom of the movable frame is connected to a connecting pipe, and the other end of the connecting pipe is connected to a heating device. The outer side of the heating device is fixedly connected to the lower plate of the printing platform.

[0030] As an optional solution for the isothermal bio-3D printing device considering cell activity described in this invention, an electric telescopic column is fixedly connected to the bottom of the moving frame, and the bottom of the electric telescopic column is fixedly connected to the lower plate of the printing platform.

[0031] As an optional solution of the isothermal bio-3D printing device considering cell activity described in this invention, a fixing ring is provided on the outside of the connecting tube, a connecting rod is fixedly connected to the bottom of the fixing ring, and the bottom of the connecting rod is fixedly connected to the lower plate of the printing platform.

[0032] A spring is fixedly connected to the top of the fixed ring, and a fixed plate is fixedly connected to the other end of the spring. The inner side of the fixed plate is fixedly connected to the connecting pipe.

[0033] Precise temperature-controlled gas delivery and circulation mechanism

[0034] During the temperature control execution phase, the heating equipment injects heated gas into the connecting pipe. Through the through-hole on the inner side of the moving frame connected to the connecting pipe, the gas is evenly discharged into the heat-insulating chamber inside the moving frame. Although the top of the heat-insulating chamber is designed to accommodate the movement of the print head, the continuously introduced constant-temperature gas can form a stable thermal atmosphere inside the chamber, achieving a highly efficient heat preservation effect.

[0035] As the movable frame of the insulation module moves upward during the printing process, its preset air jet holes can maintain real-time alignment with the through holes of the movable frame to ensure a continuous and stable supply of constant-temperature gas and guarantee consistent insulation performance.

[0036] Adaptive adjustment of the moving frame and anti-tangling structure for pipelines

[0037] Driven by the electric telescopic column, the moving frame can move flexibly in the horizontal direction. By adjusting the alignment of the through hole and the air jet holes at different positions of the moving frame, targeted temperature adjustment can be implemented for the temperature control needs of different areas of the printed object, thereby improving the accuracy of temperature control.

[0038] To avoid the problem of the connecting pipe becoming tangled and messy during the movement of the moving frame, this solution adds a linkage structure of a fixing ring, spring and fixing plate: when the moving frame moves, the elastic tension of the spring can pull the connecting pipe to keep it taut; when the moving frame returns to its original position, the rebound force of the spring can drive the connecting pipe to return to its original position synchronously, effectively avoiding the interference caused by messy pipes to the operation of the printing equipment.

[0039] Compared with the prior art, the beneficial effects of the present invention are:

[0040] To address the issue of bioactivity degradation caused by temperature fluctuations in the pre-formed structure during bio-3D printing, this solution employs an integrated thermal insulation system for the printing platform. The specific technical optimizations are as follows:

[0041] Follow-up insulation design of insulation modules

[0042] An insulation module is embedded inside the printing platform. Before the printing job starts, the temperature control module preheats the printing area to ensure that the initial printing temperature matches the activity requirements of the bio-ink and cells, guaranteeing the quality of the printed structure from the outset. During printing, as the print head gradually moves upward, the linked lifting module synchronously moves the insulation module upward, ensuring that the insulation module always covers the outside of the printed object, achieving uninterrupted insulation of the printed structure throughout the entire process and effectively maintaining the bioactivity of the printed object.

[0043] Precise temperature-controlled gas delivery and circulation mechanism

[0044] During the temperature control execution phase, the heating equipment injects heated gas into the connecting pipe. Through the through-hole on the inner side of the moving frame connected to the connecting pipe, the gas is evenly discharged into the heat-insulating chamber inside the moving frame. Although the top of the heat-insulating chamber is designed to accommodate the movement of the print head, the continuously introduced constant-temperature gas can form a stable thermal atmosphere inside the chamber, achieving a highly efficient heat preservation effect.

[0045] As the movable frame of the insulation module moves upward during the printing process, its preset air jet holes can maintain real-time alignment with the through holes of the movable frame to ensure a continuous and stable supply of constant-temperature gas and guarantee consistent insulation performance.

[0046] Adaptive adjustment of the moving frame and anti-tangling structure for pipelines

[0047] Driven by the electric telescopic column, the moving frame can move flexibly in the horizontal direction. By adjusting the alignment of the through hole and the air jet holes at different positions of the moving frame, targeted temperature adjustment can be implemented for the temperature control needs of different areas of the printed object, thereby improving the accuracy of temperature control.

[0048] To avoid the problem of the connecting pipe becoming tangled and messy during the movement of the moving frame, this solution adds a linkage structure of a fixing ring, spring and fixing plate: when the moving frame moves, the elastic tension of the spring can pull the connecting pipe to keep it taut; when the moving frame returns to its original position, the rebound force of the spring can drive the connecting pipe to return to its original position synchronously, effectively avoiding the interference caused by messy pipes to the operation of the printing equipment. Attached Figure Description

[0049] Figure 1 This is a schematic diagram of the overall structure of a isothermal bio-3D printing device that takes cell viability into account.

[0050] Figure 2 This is a schematic diagram of the printing platform of a thermostatic bio-3D printing device that takes cell viability into account.

[0051] Figure 3 This is a schematic diagram of the insulation module of a thermostatic bio-3D printing device that takes cell viability into account.

[0052] Figure 4 This is a schematic diagram of the lifting module of a thermostatic bio-3D printing device that takes cell viability into account.

[0053] Figure 5 This is a schematic diagram of the temperature control module of a thermostatic bioprinting device that takes cell viability into account.

[0054] Figure 6 For a temperature-controlled bioprinting device that takes cell viability into account Figure 5 A schematic diagram of the structure at point A.

[0055] In the diagram: 1. 3D printing equipment; 2. Vertical moving mechanism; 3. Printing nozzle; 4. Horizontal moving base; 5. Printing platform; 6. Insulation module; 601. Moving frame; 602. Insulation layer; 603. Air jet; 7. Following lifting module; 701. Fixed cylinder; 702. Motor; 703. Threaded shaft; 704. Moving sleeve; 8. Temperature control module; 801. Moving frame; 802. Through hole; 803. Connecting pipe; 804. Heating equipment; 805. Electric telescopic column; 806. Connecting rod; 807. Fixing ring; 808. Spring; 809. Fixed plate. Detailed Implementation

[0056] Example 1: Please refer to Figure 1 , Figure 2 and Figure 3The present invention provides a technical solution:

[0057] A thermostatic bio-3D printing device that takes into account cell viability includes a horizontally movable base 4;

[0058] A printing platform 5 is installed above the horizontally movable base 4. An isolation and heat preservation module 6 is installed inside the printing platform 5 to protect the printed material.

[0059] The printing platform 5 is arranged in upper and lower layers;

[0060] A following lifting module 7 is fixedly connected to one side of the bottom of the insulation module 6, and the bottom of the following lifting module 7 is fixedly connected to the lower plate of the printing platform 5.

[0061] The insulation module 6 also has a temperature module 8 installed inside, and the outside of the temperature module 8 is fixedly connected to the printing platform 5.

[0062] The printing platform 5 consists of upper and lower plates, and the upper plate of the printing platform 5 is fixedly connected to the horizontal moving base 4. The horizontal moving base 4 is used to drive the printing platform 5 to move horizontally.

[0063] The insulation module 6 includes a movable frame 601, and a following lifting module 7 is fixedly connected to one side of the bottom of the movable frame 601. The bottom of the following lifting module 7 is fixedly connected to the lower plate of the printing platform 5.

[0064] An insulation layer 602 is fixedly connected to the inner side of the movable frame 601, and air jet holes 603 are opened on the inner sides of both the movable frame 601 and the insulation layer 602.

[0065] The interior of the movable frame 601 is hollow, and a temperature module 8 is provided on the inner side of the movable frame 601;

[0066] The movable frame 601 passes through the upper plate of the printing platform 5 and is slidably connected to the upper plate of the printing platform 5.

[0067] The rear side of the horizontal moving base 4 is also fixedly connected to the 3D printing device 1, and the front side of the 3D printing device 1 is fixedly connected to two sets of vertical moving mechanisms 2. A printing nozzle 3 is also installed between the vertical moving mechanisms 2.

[0068] The following lifting module 7 includes a fixed cylinder 701 that is fixedly connected to the lower plate of the printing platform 5. A motor 702 is fixedly connected inside the fixed cylinder 701. A threaded shaft 703 is fixedly connected to the end of the main shaft of the motor 702. A movable sleeve 704 is screwed to the outside of the threaded shaft 703. The top of the movable sleeve 704 is fixedly connected to the insulation module 6.

[0069] Bioprinting is often time-consuming, especially when constructing large-scale, high-precision, or multi-component composite biomimetic structures. Printing cycles can extend from several hours to tens of hours. During this lengthy process, the pre-printed structure is continuously exposed to an open environment, causing uncontrollable temperature changes over time. On one hand, the exothermic curing of bio-inks, such as the polymerization heat from photocuring and the reaction heat from ionic cross-linking, leads to localized temperature increases. On the other hand, the product is in an open environment during printing, and as heat diffuses and dissipates, the temperature of the pre-printed structure gradually decreases. This dynamic temperature fluctuation has multi-dimensional negative impacts on the biocompatibility and functional activity of the biomimetic structure.

[0070] Cell viability damage: Increased temperature can disrupt the balance of enzyme activity within cells and accelerate apoptosis; a sudden drop in temperature can damage the fluidity and integrity of the cell membrane, leading to cell inactivation and directly reducing the cell survival rate of the printed structure.

[0071] Biomaterial performance degradation: Temperature changes can alter the crosslinking density and network structure of bio-inks, causing differences in the mechanical properties of the first-printed areas, such as elastic modulus and tensile strength, compared to the later-printed areas. This results in a decrease in the mechanical uniformity of the structure and may even lead to molding defects such as interlayer delamination and cracking.

[0072] Inactivation of bioactive factors: If the bio-ink contains active substances such as growth factors and cytokines, temperature fluctuations will disrupt their spatial conformation, causing them to lose their biological function, which in turn affects the subsequent cell proliferation, differentiation and tissue regeneration capabilities of the printed structure.

[0073] To address the issue of bioactivity degradation caused by temperature fluctuations in the pre-formed structure during bio-3D printing, this solution employs an integrated thermal insulation system for the printing platform. The specific technical optimizations are as follows:

[0074] Follow-up insulation design of insulation modules

[0075] An insulation module 6 is embedded inside the printing platform 5. Before the printing job starts, the temperature control module 8 can preheat the printing area to ensure that the initial printing environment temperature matches the activity requirements of the bio-ink and cells, thus guaranteeing the forming quality of the printed structure from the source. During the printing process, as the print head 3 gradually moves upward, the linked lifting module 7 will synchronously move the insulation module 6 upward, ensuring that the insulation module 6 always covers the outside of the printed object, achieving uninterrupted insulation of the printed structure throughout the process and effectively maintaining the bioactivity of the printed object.

[0076] Also includes the following:

[0077] As the print head 3 gradually moves upward, it drives the threaded shaft 703 to rotate via the motor 702. The threaded shaft 703 drives the outer movable sleeve 704 to move, and the movable sleeve 704 drives the upper insulation module 6 to move, thereby achieving insulation of the printed material and ensuring its quality.

[0078] Example 2: This example is an improvement upon Example 1. Please refer to [link / reference]. Figure 4 and Figure 5 Specifically, the temperature module 8 includes a movable frame 801 disposed inside the insulation module 6. The inner side of the movable frame 801 is provided with evenly distributed through holes 802. The bottom of the movable frame 801 is connected to a connecting pipe 803. The other end of the connecting pipe 803 is connected to a heating device 804. The outer side of the heating device 804 is fixedly connected to the lower plate of the printing platform 5.

[0079] The bottom of the movable frame 801 is also fixedly connected to an electric telescopic column 805, and the bottom of the electric telescopic column 805 is fixedly connected to the lower plate of the printing platform 5.

[0080] A fixing ring 807 is provided on the outside of the connecting pipe 803. A connecting rod 806 is fixedly connected to the bottom of the fixing ring 807. The bottom of the connecting rod 806 is fixedly connected to the lower plate of the printing platform 5.

[0081] A spring 808 is fixedly connected to the upper part of the fixing ring 807, and a fixing plate 809 is fixedly connected to the other end of the spring 808. The inner side of the fixing plate 809 is fixedly connected to the connecting pipe 803.

[0082] Precise temperature-controlled gas delivery and circulation mechanism

[0083] During the temperature control execution phase, the heating device 804 injects heated gas into the connecting pipe 803. Through the through-hole 802 on the inner side of the moving frame 801 connected to the connecting pipe 803, the gas is evenly discharged into the heat preservation chamber inside the moving frame 601. Although the top of the heat preservation chamber is designed to accommodate the movement of the printing nozzle, the continuously introduced constant-temperature gas can form a stable thermal atmosphere inside the chamber, achieving a highly efficient heat preservation effect.

[0084] As the movable frame 601 of the insulation module 6 moves upward with the printing process, its preset air jet hole 603 can maintain real-time alignment with the through hole 802 of the movable frame 801 to ensure a continuous and stable supply of constant temperature gas and ensure the consistency of insulation effect.

[0085] Adaptive adjustment of the moving frame and anti-tangling structure for pipelines

[0086] Driven by the electric telescopic column 805, the moving frame 801 can move flexibly in the horizontal direction. By adjusting the alignment of the through hole 802 with the air jet holes 603 at different positions of the moving frame 601, targeted temperature adjustment can be implemented for the temperature control needs of different areas of the printed object, thereby improving the accuracy of temperature control.

[0087] To avoid the connecting pipe 803 becoming tangled or messy during the movement of the moving frame 801, this solution adds a linkage structure of a fixing ring 807, a spring 808, and a fixing plate 809: when the moving frame 801 moves, the elastic tension of the spring 808 can pull the connecting pipe 803 to keep it taut; when the moving frame 801 returns to its original position, the rebound force of the spring 808 can drive the connecting pipe 803 to return to its original position synchronously, effectively avoiding interference caused by messy pipes to the operation of the printing equipment.

[0088] This article uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only for the purpose of helping to understand the method and core ideas of the present invention. The above descriptions are only preferred embodiments of the present invention. It should be noted that due to the limitations of textual expression, while there are objectively infinite specific structures, those skilled in the art can make several improvements, modifications, or changes without departing from the principles of the present invention, and can also combine the above technical features in an appropriate manner. These improvements, modifications, changes, or combinations, or the direct application of the inventive concept and technical solution to other situations without modification, should all be considered within the scope of protection of the present invention.

Claims

1. A thermostatic bio-3D printing device that takes into account cell viability, characterized in that: Including the horizontally movable base (4); A printing platform (5) is installed above the horizontally movable base (4). An isolation and heat preservation module (6) is installed inside the printing platform (5). The isolation and heat preservation module (6) is used to protect the printed material. The printing platform (5) is arranged in upper and lower layers; A following lifting module (7) is fixedly connected to one side of the bottom of the insulation module (6), and the bottom of the following lifting module (7) is fixedly connected to the lower plate of the printing platform (5). The insulation module (6) also has a temperature module (8) installed inside, and the outside of the temperature module (8) has a printing platform (5) fixedly connected.

2. The isothermal bio-3D printing device considering cell viability according to claim 1, characterized in that: The printing platform (5) is composed of upper and lower plates, and the upper plate of the printing platform (5) is fixedly connected to the horizontal moving base (4). The horizontal moving base (4) is used to drive the printing platform (5) to move horizontally.

3. The isothermal bio-3D printing device considering cell viability according to claim 1, characterized in that: The insulation module (6) includes a movable frame (601), and a following lifting module (7) is fixedly connected to one side of the bottom of the movable frame (601). The bottom of the following lifting module (7) is fixedly connected to the lower plate of the printing platform (5). An insulation layer (602) is fixedly connected to the inner side of the movable frame (601), and air vents (603) are opened on the inner sides of both the movable frame (601) and the insulation layer (602). The interior of the movable frame (601) is hollow, and a temperature module (8) is provided on the inner side of the movable frame (601). The movable frame (601) passes through the upper plate of the printing platform (5) and is slidably connected to the upper plate of the printing platform (5).

4. The isothermal bio-3D printing device considering cell viability according to claim 1, characterized in that: The rear side of the horizontal moving base (4) is also fixedly connected to the 3D printing equipment (1), and the front side of the 3D printing equipment (1) is fixedly connected to two sets of vertical moving mechanisms (2). A printing nozzle (3) is also installed between the vertical moving mechanisms (2).

5. The isothermal bio-3D printing device considering cell viability according to claim 1, characterized in that: The following lifting module (7) includes a fixed cylinder (701) that is fixedly connected to the lower plate of the printing platform (5). A motor (702) is fixedly connected inside the fixed cylinder (701). A threaded shaft (703) is fixedly connected to the end of the main shaft of the motor (702). A movable sleeve (704) is screwed to the outside of the threaded shaft (703). The top of the movable sleeve (704) is fixedly connected to the insulation module (6).

6. The isothermal bio-3D printing device considering cell viability according to claim 1, characterized in that: The temperature module (8) includes a movable frame (801) disposed inside the insulation module (6). The movable frame (801) has evenly distributed through holes (802) on its inner side. The bottom of the movable frame (801) is connected to a connecting pipe (803). The other end of the connecting pipe (803) is connected to a heating device (804). The outer side of the heating device (804) is fixedly connected to the lower plate of the printing platform (5).

7. The isothermal bio-3D printing device considering cell viability according to claim 6, characterized in that: The bottom of the movable frame (801) is also fixedly connected to an electric telescopic column (805), and the bottom of the electric telescopic column (805) is fixedly connected to the lower plate of the printing platform (5).

8. A isothermal bio-3D printing device considering cell viability according to claim 6, characterized in that: A fixing ring (807) is provided on the outside of the connecting pipe (803), and a connecting rod (806) is fixedly connected to the bottom of the fixing ring (807). The bottom of the connecting rod (806) is fixedly connected to the lower plate of the printing platform (5). A spring (808) is fixedly connected to the top of the fixed ring (807), and a fixed plate (809) is fixedly connected to the other end of the spring (808). The inner side of the fixed plate (809) is fixedly connected to the connecting pipe (803).

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