A method for efficiently expressing and producing BMP-2 in escherichia coli
By introducing an expression plasmid that helps form disulfide bonds and a codon-optimized nucleic acid sequence into E. coli, combined with a high-density fermentation strategy, efficient soluble expression of BMP-2 was achieved, solving the problems of low yield and high cost, and realizing efficient and low-cost industrial production.
CN122146731APending Publication Date: 2026-06-05BEIJING GENETECH PHARML
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- BEIJING GENETECH PHARML
- Filing Date
- 2026-01-27
- Publication Date
- 2026-06-05
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Figure CN122146731A_ABST
Abstract
The application provides a method for efficiently expressing and producing BMP-2 in Escherichia coli. The synergistic effect of the host cell, the BMP-2 fusion protein and the high-density fermentation and induction strategy optimized in the application promotes the high-level expression of the target protein in a soluble and active form, so that the BMP-2 fusion protein accounts for more than 30% of the total soluble protein, the volume yield of the fusion protein in the fermentation liquor reaches an amazing yield of 26 g / L, and the fusion protein has activity after purification. The application simplifies the downstream purification process, reduces the production cost, and fundamentally overcomes the key bottleneck of inclusion body formation existing in the traditional method.
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