Method for identifying realness of scutellaria baicalensis in pediatric re speed clear syrup

Abstract

The application relates to the field of analytical detection technology and discloses a method for identifying the authenticity of baicalin raw materials in Xiaoer Re Shu Qing syrup, which comprises the following steps: performing high performance liquid chromatography detection on the Xiaoer Re Shu Qing syrup to obtain a chromatogram, recording the peak areas of baicalin and wogonoside in the chromatogram, and calculating an F value; the F value is the ratio of the peak area of wogonoside to the peak area of baicalin; if the F value is less than 0.26, the Xiaoer Re Shu Qing syrup contains baicalin extract adulteration. The above technical scheme solves the problem of adding baicalin extract in the Xiaoer Re Shu Qing syrup in the prior art.

Description

Technical Field

[0001] This invention relates to the field of analytical testing technology, specifically to a method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup. Background Technology

[0002] Xiaor Resuqing Syrup, a traditional Chinese medicine for treating childhood influenza, is composed of eight herbs: Bupleurum, Isatis root, Honeysuckle, Forsythia, Scutellaria, Kudzu root, Rhubarb, and Buffalo horn. It can exert antiviral, antibacterial, antipyretic, and anti-inflammatory effects. It has a strong antipyretic and detoxifying effect on various types of exogenous high fever in children, including wind-heat syndrome and internal and external heat syndrome. Scutellaria in the formula has the function of clearing heat and drying dampness, clearing lung heat in the upper jiao, and is effective for symptoms such as fever, sore throat, and cough in wind-heat colds.

[0003] Currently, the Chinese Pharmacopoeia's standard for children's quick-clearing syrup only specifies the identification and content of baicalin in its provisions regarding the use of Scutellaria baicalensis, resulting in a limited range of indicator compounds. Since the cost of using Scutellaria baicalensis extract is only half that of using Scutellaria baicalensis slices, some companies may add Scutellaria baicalensis extract to reduce costs, potentially altering its material composition. There is no corresponding clinical data to confirm its effectiveness, posing a safety risk. Therefore, a method is urgently needed to verify the authenticity of Scutellaria baicalensis in children's quick-clearing syrup. Summary of the Invention

[0004] This invention proposes a method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup, which solves the problem of adulteration by adding Scutellaria baicalensis extract to children's quick-clearing syrup in related technologies.

[0005] The technical solution of the present invention is as follows: This invention proposes a method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup, comprising the following steps: High performance liquid chromatography was used to detect the pediatric heat-clearing syrup, and the chromatogram was obtained. The peak areas of baicalin and wogonin in the chromatogram were recorded, and the F value was calculated. The F value is the ratio of the peak area of ​​baicalin to the peak area of ​​scutellarin; if the F value is <0.26, then the pediatric heat-clearing syrup contains adulterated scutellaria extract.

[0006] As a further technical solution, if the F value is <0.10, then the pediatric heat-clearing syrup contains Scutellaria baicalensis extract as adulteration and the amount of adulteration is >50wt%.

[0007] As a further technical solution, the chromatographic conditions for high-performance liquid chromatography detection include: The mobile phase includes mobile phase A and mobile phase B; The mobile phase A is acetonitrile, and the mobile phase B is an aqueous solution of phosphoric acid.

[0008] As a further technical solution, the high-performance liquid chromatography (HPLC) detection employs gradient elution, and the gradient elution procedure includes: From 0 min to 15 min, the volume fraction of mobile phase A changed from 10% to 15%, and the volume fraction of mobile phase B changed from 90% to 85%. From 15 min to 40 min, the volume fraction of mobile phase A changed from 15% to 30%, and the volume fraction of mobile phase B changed from 85% to 70%. For 40-50 minutes, the volume fraction of mobile phase A is 30% and the volume fraction of mobile phase B is 70%.

[0009] In this invention, by optimizing the gradient elution procedure during high-performance liquid chromatography (HPLC) detection, baicalin and wogonin are well separated, and the chromatogram of the test solution is free of impurity peaks.

[0010] As a further technical solution, the volume fraction of the phosphoric acid aqueous solution is 0.08%~0.12%.

[0011] As a further technical solution, the chromatogram includes two common peaks, wherein peak 1 is baicalin and peak 2 is wogonin.

[0012] As a further technical solution, the high-performance liquid chromatography detection includes a test solution, a mixed reference solution, and a Scutellaria baicalensis reference herb solution; The method for preparing the test solution includes the following steps: Take pediatric fever-reducing syrup, scutellaria baicalensis extract, or standard preparation, add solvent, filter, and prepare the test solution; The method for preparing the mixed reference solution includes the following steps: Baicalin and wogonin reference standards were dissolved separately in solvents to obtain baicalin reference standard stock solution and wogonin reference standard stock solution. The baicalin and wogonin reference standard stock solutions were mixed to obtain a mixed reference standard solution. The preparation method of the Scutellaria baicalensis reference herb solution includes the following steps: Take Scutellaria baicalensis as a reference herb, decoct it in water, filter it, concentrate it, add solvent, filter it again, and obtain a Scutellaria baicalensis reference herb solution.

[0013] As a further technical solution, the concentration of baicalin in the mixed reference solution is 104.94 μg / mL, and the concentration of wogonin is 106.56 μg / mL.

[0014] As a further technical solution, the solvents used in preparing the test solution, the mixed reference solution, and the Scutellaria baicalensis reference herb solution each independently include methanol or ethanol.

[0015] As a further technical solution, the volume fractions of methanol and ethanol are each independently 50% to 100%.

[0016] As a further technical solution, the high-performance liquid chromatography uses octadecylsilane-bonded silica gel as the packing material, with a column length of 250 mm, an inner diameter of 4.6 mm, a particle size of 5 μm, a column temperature of 28~32℃, a flow rate of 0.8~1.2 mL / min, and an injection volume of 5~10 μL. Preferably, the column temperature is 30℃, the flow rate is 1mL / min, and the injection volume is 10μL.

[0017] The working principle and beneficial effects of this invention are as follows: This invention provides a method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup. It can determine the peak area ratio of baicalin and scutellarin in children's quick-clearing syrup, and screen for the problem of Scutellaria baicalensis extract replacing Scutellaria baicalensis. The determination method in this invention has good stability, repeatability, precision and reliability of HPLC detection conditions, and the detection results are accurate. It is suitable for large-scale detection of children's quick-clearing syrup.

[0018] This invention employs high-performance liquid chromatography (HPLC) to determine the peak area ratio of baicalin and scutellarin in children's quick-clearing syrup. Experimental data demonstrate that this invention meets the requirements for methodological validation and is feasible. The determination method in this invention can quickly and conveniently detect the authenticity of scutellaria baicalensis in children's quick-clearing syrup, providing a basis for quality control of the scutellaria baicalensis flavor in the syrup. The preparation process of children's quick-clearing syrup can be monitored based on the test results, thereby improving product quality. Attached Figure Description

[0019] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0020] Figure 1 This is a chromatogram of the test solution of the Scutellaria baicalensis negative standard preparation in Example 1 of the present invention; Figure 2 The chromatogram of the test solution of the standard preparation of Pediatric Heat-Clearing Syrup in Example 1 of the present invention is shown, wherein peak 1 is baicalin and peak 2 is wogonin. Figure 3 The chromatogram of the Scutellaria baicalensis reference herb solution in Example 1 of the present invention is shown, wherein peak 1 is baicalin and peak 2 is wogonin. Figure 4 The chromatogram of the mixed reference solution in Example 1 of the present invention is shown, wherein peak 1 is baicalin and peak 2 is wogonin. Figure 5 This is a chromatogram of the Scutellaria baicalensis extract solution in Example 1 of the present invention, wherein peak 1 is baicalin; Figure 6 This is a chromatogram of the positive standard preparation of Scutellaria baicalensis extract in Example 1 of the present invention, wherein peak 1 is baicalin; Figure 7 This is a linear relationship graph between the proportion of Scutellaria baicalensis extract and the F ratio in Example 2 of the present invention; Figure 8 This is the chromatogram of the test solution in Example 4 of the present invention; Figure 9 This is the chromatogram of the test solution in Example 5 of the present invention; Figure 10 This is a chromatogram of the test solution from Example 6 of the present invention. Detailed Implementation

[0021] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0022] The raw materials and instruments used in the following examples and comparative examples are as follows: Column: Cosmosil Packed 5C18-MS-II (4.6mm × 250mm, 5μm); Baicalin reference standard: Batch number: 110715-202223, China National Institutes for Food and Drug Control, purity: 97.2%; Baicalin reference standard: Batch number: 112002-202303, China National Institutes for Food and Drug Control, purity: 98.5%; Scutellaria baicalensis reference material: batch number 120955-201810, China National Institutes for Food and Drug Control; Acetonitrile: chromatographic grade; Phosphoric acid: chromatographic grade; Methanol: analytical grade; The water is purified water; Scutellaria baicalensis extract, purity 88.0%.

[0023] Example 1 (1) Standard formulation The preparation method of the standard formulation of pediatric fever-reducing syrup includes the following steps: Weigh 25g of Bupleurum chinense slices, 18.75g of Lonicera japonica slices, and 15g of Forsythia suspensa slices, place them in a volatile oil extractor, soak for 30 minutes, and distill to extract the volatile oil, obtaining volatile oil, distilled water solution, and dregs; weigh 6.25g of buffalo horn, add 300mL of water and decoct for 3 hours, then add the above dregs, 25g of Isatis indigotica root, 12.5g of Scutellaria baicalensis root, 12.5g of Pueraria lobata root, and 6.25g of Rheum palmatum root, add 300mL of water and decoct twice, 1 hour each time, combine the decoctions, filter, combine the filtrate with the above distilled water solution, concentrate, add the above volatile oil, stir well, adjust the volume to 100mL, and obtain the standard preparation of Pediatric Heat-Clearing Syrup; The preparation process of the Scutellaria baicalensis negative standard preparation includes the following steps: Weigh 25g of Bupleurum chinense slices, 18.75g of Lonicera japonica slices, and 15g of Forsythia suspensa slices, place them in a volatile oil extractor, soak for 30 minutes, and distill to extract the volatile oil, obtaining volatile oil, distilled water solution, and dregs; weigh 6.25g of buffalo horn, add 300mL of water and decoct for 3 hours, then add the above dregs, 25g of Isatis indigotica root, 12.5g of Pueraria lobata root, and 6.25g of Rheum palmatum root, add 300mL of water and decoct twice, 1 hour each time, combine the decoctions, filter, combine the filtrate with the above distilled water solution, concentrate, add volatile oil, stir well, and adjust the volume to 100mL to obtain the Scutellaria baicalensis negative standard preparation; The preparation process of the positive standard preparation of Scutellaria baicalensis extract includes the following steps: Weigh 25g of Bupleurum chinense slices, 18.75g of Lonicera japonica slices, and 15g of Forsythia suspensa slices, place them in a volatile oil extractor, soak for 30 minutes, and distill to extract the volatile oil, obtaining volatile oil, distilled water solution, and dregs; weigh 6.25g of buffalo horn, add 300mL of water and decoct for 3 hours, then add the above dregs, 25g of Isatis indigotica root, 12.5g of Pueraria lobata root, and 6.25g of Rheum palmatum root, add 300mL of water and decoct twice, 1 hour each time, combine the decoctions, filter, combine the filtrate with the above distilled water solution, concentrate, add volatile oil, stir well, adjust the volume to 100mL, add 0.042g of Scutellaria baicalensis extract, and obtain a positive standard preparation of Scutellaria baicalensis extract; The preparation process of positive standard preparations of Scutellaria baicalensis extracts in different proportions includes the following steps: Bupleurum, honeysuckle, and forsythia were weighed and placed in a volatile oil extractor. They were soaked for 30 minutes and the volatile oil was extracted by distillation to obtain volatile oil, distilled water solution, and dregs. Buffalo horn was weighed, and 150 mL of water was added to decoct it for 3 hours. Then, the above dregs, Isatis root, Scutellaria, Pueraria, and rhubarb were added to 150 mL of water and decocted twice, 1 hour each time. The decoctions were combined, filtered, and the filtrate was combined with the above distilled water solution. The solution was concentrated, volatile oil was added, and the mixture was stirred well. The volume was adjusted to 50 mL, and Scutellaria extract was added to obtain positive standard preparations of Scutellaria extract in different proportions. The dosage of each herb is shown in Tables 1-2 below. Table 1. Formulations of positive standard preparations of Scutellaria baicalensis extract at different proportions (0%~75%)

[0024] Table 2. Formulations of positive standard preparations with different proportions (90%~100%) of Scutellaria baicalensis extract.

[0025] (2) Test solution The preparation process of the pediatric fever-reducing syrup test solution includes the following steps: Accurately measure 1 mL of Pediatric Heat-Clearing Syrup and place it in a 100 mL volumetric flask. Dilute it to the mark with a 50% (v / v) methanol aqueous solution, shake well, and filter through a 0.45 μm microporous membrane to obtain the Pediatric Heat-Clearing Syrup test solution. The preparation process of the test solution for the standard preparation of Pediatric Fever-Clearing Syrup includes the following steps: Accurately measure 1 mL of the standard preparation of Pediatric Relief Syrup and place it in a 100 mL volumetric flask. Add 50% methanol aqueous solution (volume fraction) to dilute to the mark, shake well, and filter through a 0.45 μm microporous membrane to obtain the test solution of the standard preparation of Pediatric Relief Syrup. The preparation process of the Scutellaria baicalensis negative standard preparation test solution includes the following steps: Accurately measure 1 mL of Scutellaria baicalensis negative standard preparation and place it in a 100 mL volumetric flask. Add 50% methanol aqueous solution (volume fraction) to dilute to the mark, shake well, and filter through a 0.45 μm microporous membrane to obtain the Scutellaria baicalensis negative standard preparation test solution. The preparation process of Scutellaria baicalensis extract solution includes the following steps: Accurately weigh 13.821 mg of Scutellaria baicalensis extract, place it in a 100 mL volumetric flask, add an appropriate amount of methanol to dissolve and dilute to the mark, shake well, and filter through a 0.45 μm microporous membrane to obtain Scutellaria baicalensis extract solution. The preparation process of the positive standard preparation test solution of Scutellaria baicalensis extract includes the following steps: Accurately measure 1 mL of the positive standard preparation of Scutellaria baicalensis extract and place it in a 100 mL volumetric flask. Add 50% methanol aqueous solution (volume fraction) to dilute to the mark, shake well, and filter through a 0.45 μm microporous membrane to obtain the test solution of the positive standard preparation of Scutellaria baicalensis extract. The preparation process of positive standard test solutions of Scutellaria baicalensis extract with different proportions includes the following steps: Accurately measure 1 mL of positive standard preparations of Scutellaria baicalensis extract in different proportions and place them in a 100 mL volumetric flask. Add 50% methanol aqueous solvent (volume fraction) to dilute to the mark, shake well, and filter through a 0.45 μm microporous membrane to obtain test solutions of positive standard preparations of Scutellaria baicalensis extract in different proportions. (3) Mixed reference solution The method for preparing a mixed reference solution includes the following steps: Accurately weigh appropriate amounts of baicalin and wogonin reference standards, and dissolve them separately in 100% methanol to obtain baicalin reference standard stock solutions with a concentration of 209.87 μg / mL and wogonin reference standard stock solutions with a concentration of 213.11 μg / mL. Accurately pipette the baicalin and wogonin reference standard stock solutions to prepare mixed reference standard solutions with concentrations of 104.94 μg / mL and 106.56 μg / mL, respectively. (4) Preparation of Scutellaria baicalensis reference herb solution Take 0.1g of Scutellaria baicalensis reference material, decoct in water for 1.5 hours, filter, concentrate the filtrate to near dryness, accurately add 25mL of 50% ethanol solution, seal tightly, weigh, sonicate (350W power, 37kHz frequency) for 45 minutes, remove, cool, weigh again, replenish the lost weight with 50% ethanol solution, shake well, filter, filter the filtrate through a 0.45μm microporous membrane to obtain Scutellaria baicalensis reference material solution; (5) High performance liquid chromatography detection conditions: Octadecylsilane bonded silica gel was used as the packing material (column length 250 mm, inner diameter 4.6 mm, particle size 5 μm), acetonitrile was used as mobile phase A, and 0.1% phosphoric acid solution was used as mobile phase B. Gradient elution was performed, the detection wavelength was 274 nm, the flow rate was 1 mL / min, the column temperature was 30 °C, the injection volume was 10 μL, and the gradient elution program is shown in Table 3 below; Table 3 Gradient elution program

[0026] Example 2 (1) Specificity verification The following solutions were injected into a high-performance liquid chromatograph (HPLC) for analysis: negative standard preparation of Scutellaria baicalensis, standard preparation of pediatric fever-reducing syrup, reference herb solution of Scutellaria baicalensis, extract solution of Scutellaria baicalensis, positive standard preparation of extract of Scutellaria baicalensis, and mixed reference solution. The results are shown in the table below. Figures 1-6 Peaks 1 and 2 in the Pediatric Fever-Clearing Syrup solution both belong to Scutellaria baicalensis. Peak 1 is baicalin and peak 2 is wogonin. The chromatogram of the Scutellaria baicalensis negative standard preparation test solution does not show the same chromatogram retention time as the Scutellaria baicalensis reference solution and the mixed reference solution, indicating that the method has good specificity. Record the peak areas of baicalin and wogonin in the chromatograms and calculate the value of F (wogonin peak area / baicalin peak area). In the Scutellaria baicalensis extract solution and the Scutellaria baicalensis extract positive standard preparation test solution, F is <0.10; in the Scutellaria baicalensis reference herb solution and the pediatric fever-reducing syrup standard preparation test solution, F is >0.20. (2) Linearity and range Accurately pipette the baicalin reference standard stock solution and the wogonin reference standard stock solution to prepare mixed reference standard solution I with concentrations of 104.94 μg / mL and 106.56 μg / mL, respectively; Accurately pipette 5 mL of mixed reference solution I into a 10 mL volumetric flask, add 100% methanol to the mark, shake well, and this is called mixed reference solution II; Accurately pipette 5 mL of mixed reference solution II into a 10 mL volumetric flask, add 100% methanol to the mark, shake well, and this is called mixed reference solution III; Accurately pipette 5 mL of mixed reference solution III into a 10 mL volumetric flask, add 100% methanol to the mark, shake well, and this is counted as mixed reference solution IV. Accurately pipette 2 mL of mixed reference solution IV into a 10 mL volumetric flask, add 100% methanol to the mark, shake well, and record as mixed reference solution V; Accurately pipette 4 mL of mixed reference solution V into a 10 mL volumetric flask, add 100% methanol to the mark, shake well, and this is recorded as mixed reference solution VI. Accurately pipette 5 mL of mixed reference solution VI into a 10 mL volumetric flask, add 100% methanol to the mark, shake well, and this is called mixed reference solution VII. Accurately pipette 10 μL each of the mixed reference solutions I to VII and inject them into the high performance liquid chromatograph. Plot a standard curve with the concentration of each reference solution (μg / mL) as the abscissa and the peak area as the ordinate. The results are shown in Table 4. Table 4. Linear relationships and linear ranges of the two components.

[0027] Accurately pipette 10 μL of each of the positive standard solutions containing different proportions of Scutellaria baicalensis extract and inject them into the high-performance liquid chromatograph. Record the peak areas of baicalin and wogonin in the characteristic chromatograms. Plot a standard curve with the value of F on the ordinate and the proportion of Scutellaria baicalensis extract added on the abscissa. Figure 7 The regression equation was calculated, and the linear relationship was good. The results are shown in Table 5. Table 5. Linearity of Positive Standard Preparations of Scutellaria baicalensis Extract with Different Proportions

[0028] (3) Repeatability test Six parallel aliquots of the prepared test solution were injected into the high-performance liquid chromatograph. The peak area ratio F of baicalin and wogonin was calculated. The mean F value was 0.34 and the RSD was 0.50% (n=6). See Table 6 for details. Table 6 Repeatability Tests

[0029] (4) Precision The standard solution of pediatric fever-reducing syrup was injected into the high-performance liquid chromatograph (HPLC) six times consecutively. The peak area ratio F of baicalin and wogonin was calculated. The mean F value was 0.28, and the RSD was 0.0% (n=6). See Table 7 for details. The results show that the instrument has good precision. Table 7 Precision

[0030] (5) Recovery rate Accurately measure 0.5 mL of the pediatric heat-clearing syrup test solution, add appropriate amounts of baicalin and wogonin reference standards, inject into the high performance liquid chromatograph, calculate the recovery rate, and the results are shown in Table 8, indicating that the recovery rate of this method is good. Table 8 Recovery Rate

[0031] (6) Stability The standard preparation solution of pediatric heat-clearing syrup was injected once at 0h, 1h, 3h, 6h, 9h, 12h and 24h respectively. The peak area ratio F of baicalin peak and wogonin peak was calculated. The mean F value was 0.34 and the RSD was 2.35% (n=7). See Table 9 for details. The results show that the instrument has good stability.

[0032] Table 9. Stability test results

[0033] Example 3 This embodiment involves the establishment of limits for the peak area ratio of baicalin and scutellarin in chromatograms, and screening for the possibility of using scutellaria extract to replace scutellaria. In the Scutellaria baicalensis extract solution and the Scutellaria baicalensis extract positive standard preparation test solution, F was <0.10; in the Scutellaria baicalensis reference herb solution and the pediatric fever-reducing syrup standard preparation test solution, F was >0.20. The peak area ratios of baicalin and scutellarin in positive standard solutions of Scutellaria baicalensis extract at different proportions showed that the peak area ratio of baicalin to scutellarin in a children's quick-clearing syrup made from 75% Scutellaria baicalensis and 25% Scutellaria baicalensis extract was approximately 0.18. Since this ratio is close to 0.20, and considering the regional differences in Scutellaria baicalensis from different producing areas and the limitations of sample collection, setting the limit at 0.20 would easily lead to misinterpretation of the results. Therefore, it is recommended to temporarily relax the limit and set it at 0.10, that is, theoretically, adding more than 50% Scutellaria baicalensis extract.

[0034] Example 4 The only difference between this embodiment and Embodiment 1 is the gradient elution procedure, as shown in Table 10 below: Table 10 Gradient Elution Table

[0035] See results Figure 8 As shown, the baicalin chromatographic peak under this elution gradient exhibits tailing.

[0036] Example 5 The only difference between this embodiment and Embodiment 1 is the gradient elution procedure, as shown in Table 11 below: Table 11 Gradient Elution Table

[0037] See results Figure 9 As shown, the chromatographic peak resolution of baicalin under this elution gradient is poor.

[0038] Example 6 The only difference between this embodiment and Embodiment 1 is the gradient elution procedure, as shown in Table 12 below: Table 12 Gradient Elution Table

[0039] The results are as follows Figure 10 As shown, the baicalin chromatographic peak under this elution gradient exhibits tailing, indicating poor resolution of the baicalin chromatographic peak.

[0040] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup, characterized in that, Includes the following steps: High performance liquid chromatography was used to detect the pediatric heat-clearing syrup, and the chromatogram was obtained. The peak areas of baicalin and wogonin in the chromatogram were recorded, and the F value was calculated. The F value is the ratio of the peak area of ​​baicalin to the peak area of ​​scutellarin; if the F value is <0.26, then the pediatric heat-clearing syrup contains adulterated scutellaria extract.

2. The method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup according to claim 1, characterized in that, If the F value is less than 0.10, then the pediatric heat-clearing syrup contains Scutellaria baicalensis extract and the amount of adulteration is greater than 50 wt%.

3. The method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup according to claim 1, characterized in that, The chromatographic conditions for high-performance liquid chromatography detection include: The mobile phase includes mobile phase A and mobile phase B; The mobile phase A is acetonitrile, and the mobile phase B is an aqueous solution of phosphoric acid.

4. The method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup according to claim 1, characterized in that, The high-performance liquid chromatography (HPLC) detection employs gradient elution, and the gradient elution procedure includes: From 0 min to 15 min, the volume fraction of mobile phase A changed from 10% to 15%, and the volume fraction of mobile phase B changed from 90% to 85%. From 15 min to 40 min, the volume fraction of mobile phase A changed from 15% to 30%, and the volume fraction of mobile phase B changed from 85% to 70%. For 40-50 minutes, the volume fraction of mobile phase A is 30% and the volume fraction of mobile phase B is 70%.

5. The method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup according to claim 3, characterized in that, The volume fraction of the phosphoric acid aqueous solution is 0.08%~0.12%.

6. The method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup according to claim 1, characterized in that, The chromatogram includes two common peaks, where peak 1 is baicalin and peak 2 is wogonin.

7. The method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup according to claim 1, characterized in that, The high-performance liquid chromatography detection includes the test solution, the mixed reference solution, and the Scutellaria baicalensis reference herb solution; The method for preparing the test solution includes the following steps: Take pediatric fever-reducing syrup, scutellaria baicalensis extract, or standard preparation, add solvent, filter, and prepare the test solution; The method for preparing the mixed reference solution includes the following steps: Baicalin and wogonin reference standards were dissolved separately in solvents to obtain baicalin reference standard stock solution and wogonin reference standard stock solution. The baicalin and wogonin reference standard stock solutions were mixed to obtain a mixed reference standard solution. The preparation method of the Scutellaria baicalensis reference herb solution includes the following steps: Take Scutellaria baicalensis as a reference herb, decoct it in water, filter it, concentrate it, add solvent, filter it again, and obtain a Scutellaria baicalensis reference herb solution.

8. The method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup according to claim 7, characterized in that, In the mixed reference solution, the concentration of baicalin was 104.94 μg / mL and the concentration of wogonin was 106.56 μg / mL.

9. The method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup according to claim 8, characterized in that, The solvents used in preparing the test solution, the mixed reference solution, and the Scutellaria baicalensis reference herb solution are each independently either methanol or ethanol.

10. The method for identifying the authenticity of Scutellaria baicalensis in children's quick-clearing syrup according to claim 9, characterized in that, The volume fractions of methanol and ethanol are each independently 50% to 100%.

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