A fungicidal composition containing boscalid and fludioxonil and use thereof

Abstract

The present application relates to the technical field of pesticide compound, especially to a kind of fungicidal composition containing boscalid and fludioxonil and application thereof;A kind of fungicidal composition containing boscalid and fludioxonil, the active ingredient contained in the fungicidal composition is boscalid and fludioxonil;The mass ratio of the boscalid and fludioxonil is 10:20~29:1;The present application discloses a kind of fungicidal composition containing boscalid and fludioxonil and application thereof, the boscalid and fludioxonil added within a certain ratio range show good synergistic effect to tomato botrytis blight, the fungicidal effect of the combination of the two is obviously higher than single agent, and the co-toxicity coefficient is above 120, the fungicidal composition prepared not only reduces its use dose and cost, but also reduces pesticide residue, reduces the harm to environment.

Description

Technical Field

[0001] This invention relates to the technical field of pesticide compound formulation, and in particular to a fungicide composition containing cyazofamid and fludioxonil and its application. Background Technology

[0002] In the field of agricultural fungal disease control, tomato gray mold (Botrytis cinerea) has become a core obstacle restricting tomato yield and quality due to its rapid outbreak in high humidity, wide host range, and latent infection characteristics. Its control is highly dependent on chemical fungicides.

[0003] Currently, boscalid and fludioxonil are commonly used single agents for the control of this disease, and their application is widespread but has significant limitations. Boscalid is a nicotinamide fungicide that blocks the energy supply of the tricarboxylic acid cycle by inhibiting the activity of mitochondrial succinate dehydrogenase (SDH) in pathogens. However, when used alone, its single target can easily induce adaptive mutations in the SdhB gene of gray mold with long-term application, leading to decreased target sensitivity and a gradual decrease in fungicidal efficiency. Fludioxonil inhibits mycelial growth and spore germination by interfering with the autophosphorylation of histidine kinase in the fungal HOG MAPK signaling pathway. However, its resistance mechanism involves multiple regulation, including overexpression of osmotic stress response genes and enhanced activity of cytochrome P450 metabolic detoxification enzymes. Using it alone not only affects the efficacy due to target fluctuations but also accelerates the screening and spread of low-level resistant strains in the field, making it difficult to meet the needs of continuous control of gray mold.

[0004] Existing compound formulations for controlling tomato gray mold mainly combine boscalid or fludioxonil with other types of fungicides, attempting to enhance control efficacy through multi-mechanism synergy. However, such formulations have inherent drawbacks. For example, the combined agents have short-lasting effects, require frequent application, and increase production costs and environmental residue risks. Furthermore, most compound formulations do not fully consider the differences in tomato growth stages, such as changes in tissue sensitivity during flowering and resistance during fruiting, leading to unstable control efficacy in actual practice. This makes it difficult to achieve the comprehensive goals of high efficiency, low resistance, and safety, thus hindering the promotion of green control technologies for gray mold.

[0005] Therefore, existing common formulations that use a single agent such as boscalid, fludioxonil, or a combination of both with other agents all face problems such as decreased bactericidal efficiency as resistance increases, high risk of cross-resistance, short duration of effect, and poor environmental adaptability, making it difficult to meet the needs for efficient and sustainable control of tomato gray mold. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a bactericidal composition containing cyazofamid and fludioxonil, and its application.

[0007] In a first aspect, the present invention provides a bactericidal composition containing cyazofamid and fludioxonil, employing the following technical solution: A bactericidal composition containing cyazofamid and fludioxonil, wherein the active ingredients of the bactericidal composition are cyazofamid and fludioxonil.

[0008] By adopting the above technical solution, the present invention has a significant synergistic effect on tomato gray mold by combining cyazofamid and fludioxonil. It can also improve the speed and duration of action. The prepared fungicidal composition is safe and environmentally friendly and can greatly reduce the cost of pesticide use.

[0009] Through multiple indoor toxicity tests and field efficacy trials, this invention has found that cyprodinil and fludioxonil, within a certain compound ratio range, have a significant synergistic effect on tomato gray mold, and the fungicidal effect of the combination of the two is significantly higher than that of the single agent.

[0010] Preferably, the mass ratio of cyazofamid to fludioxonil is 10:20 to 29:1, for example, 10:20, 11:19, 12:18, 13:17, 14:16, 15:15, 16:14, 17:13, 18:12, 19:11, 20:10, 21:9, 22:8, 23:7, 24:6, 25:5, 26:4, 27:3, 28:2 or 29:1.

[0011] More preferably, the mass ratio of cyazofamid to fludioxonil is 14:16 to 29:1; for example, 14:16, 15:15, 16:14, 17:13, 18:12, 19:11, 20:10, 21:9, 22:8, 23:7, 24:6, 25:5, 26:4, 27:3, 28:2 or 29:1.

[0012] More preferably, the mass ratio of cyazofamid to fludioxonil is 22:8 to 28:2; for example, 22:8, 23:7, 24:6, 25:5, 26:4, 27:3 or 28:2.

[0013] Preferably, the sum of the masses of cyazofamid and fludioxonil in the bactericidal composition is 1% to 60% of the total mass of the bactericidal composition; for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%.

[0014] More preferably, the sum of the masses of cyazofamid and fludioxonil in the bactericidal composition is 1% to 40% of the total mass of the bactericidal composition; for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, or 40%.

[0015] More preferably, the sum of the masses of cyazofamid and fludioxonil in the bactericidal composition is 1% to 30% of the total mass of the bactericidal composition, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, or 30%.

[0016] Preferably, the bactericidal composition further includes adjuvants and / or fillers that are permissible and acceptable in pesticide science.

[0017] Preferably, the adjuvant ingredients permitted and acceptable in the pesticide science include, but are not limited to, penetrants, dispersants, diffusion agents, emulsifiers, stabilizers, wetting agents, thickeners, defoamers, antifreeze agents, or synergists.

[0018] Preferably, the filler is water.

[0019] Preferably, the bactericidal composition is prepared into an agriculturally permissible formulation; the formulation is a suspension, microcapsule suspension, wettable powder, emulsifiable concentrate, microemulsion, soluble concentrate, water emulsion, water-dispersible granules, suspension emulsion, or ultra-low volume liquid.

[0020] Secondly, the present invention provides an application of a fungicide composition containing cyazofamid and fludioxonil in the prevention and control of plant diseases, employing the following technical solution: Application of a fungicide composition containing cyazofamid and fludioxonil in the control of plant diseases.

[0021] Preferably, the plant disease is tomato gray mold.

[0022] In summary, the present invention has at least one of the following beneficial technical effects: This invention discloses a fungicide composition containing cyazofamid and fludioxonil and its application. The cyazofamid and fludioxonil, when added in a certain ratio range, show a good synergistic effect against tomato gray mold. The fungicide effect of the combination of the two is significantly higher than that of the single agent, and the co-toxicity coefficient is at least 120. The fungicide composition prepared not only reduces its dosage and cost, but also reduces pesticide residues and mitigates environmental damage. Detailed Implementation

[0023] The technical solution of the present invention is further illustrated below through specific embodiments. These specific embodiments do not represent a limitation on the scope of protection of the present invention. Some non-essential modifications and adjustments made by others based on the concept of the present invention still fall within the scope of protection of the present invention.

[0024] The raw materials involved in this invention are all commercially available products. Boscalid, CAS No.: 188425-85-6; Fludioxonil, CAS No.: 131341-86-1; Tristyrylphenol polyoxyethylene ether, molecular formula, C 30 H 24 O.(C2H4O)n; CAS number: 99734-09-5; Polyoxyalkylarylphenyl ether phosphate salt, molecular formula, C6H 15 NO3.x((C2H4O)nC 30 H 30 O).x(H3PO4), CAS No.: 105362-40-1; Modified sulfonate anionic dispersant, molecular formula: C6H6O4S・C6H6O・CH4N 2O • C, CAS No.: 102980-04-1; Potassium monododecyl phosphate, molecular formula, C 12 H 25 OPO3K2, CAS No.: 39322-78-6; Sodium dialkylsulfonyl succinate, molecular formula, C 20 H37 NaO7S, CAS No.: 577-11-7; Magnesium aluminum silicate, CAS No.: 70205-22-6; Xanthan gum, CAS No.: 11138-66-2; Ethylene glycol, CAS No.: 10721-1; Carson, CAS No.: 26172-55-4; Organosilicon defoamer (modified polysiloxane), CAS No.: 9006-65-9; Rosin polyoxyethylene ether, CAS No.: 8050-33-7； The present invention will be further described in detail below with reference to embodiments and comparative examples.

[0025] Application Example 1: Indoor toxicity test of the combination of boscalid and fludioxonil against tomato gray mold 1. Drugs and test targets 1.1 Pharmacology 96% boscalid technical grade was provided by Zhejiang Heben Technology Co., Ltd.; 95% fludioxonil technical grade was provided by Zhejiang Heben Technology Co., Ltd.

[0026] 1.2 Test Target Botrytis cinerea, the pathogen of tomato gray mold, is Botrytis cinerea) Provided by the Plant Pathology Laboratory of Jiangxi Agricultural University.

[0027] 2. Experimental Procedure 2.1 Preparation of the medicine High-concentration stock solutions were prepared by dissolving boscalid and fludioxonil technical materials separately in acetone, and five groups of boscalid-fludioxonil mixtures were prepared at ratios of 14:16, 22:8, 24:6, 28:2, and 29:1. Based on the preliminary experiments, the boscalid stock solutions were diluted with acetone to concentrations of 729 μg / mL, 243 μg / mL, 81 μg / mL, 27 μg / mL, and 9 μg / mL; the fludioxonil stock solutions were diluted to concentrations of 81 μg / mL, 27 μg / mL, 9 μg / mL, 3 μg / mL, and 1 μg / mL; and the boscalid-fludioxonil mixtures were diluted to concentrations of 243 μg / mL, 81 μg / mL, 27 μg / mL, 9 μg / mL, 3 μg / mL, and 1 μg / mL.

[0028] 2.2 Chemical Treatment Under aseptic conditions, 49g of pre-melted sterile culture medium was added to a sterile Erlenmeyer flask according to the experimental treatment. 1mL of the drug solution was then added sequentially to the Erlenmeyer flasks from low to high concentration, and the mixture was thoroughly shaken. The mixture was then poured into four petri dishes to prepare agar plates containing the corresponding drug concentrations. A blank control (treatment without drug) was included, and each treatment was replicated four times.

[0029] 2.3 Vaccination Under aseptic conditions, use a 5mm diameter sterile punch to cut out a mycelial cake from the edge of the colony after the pathogen has been cultured. Inoculate the mycelial cake onto the center of the drug-containing plate with the mycelial side facing up, cover the plate, and place it in an incubator at (25±1)℃ for cultivation.

[0030] 2.4 Investigation Five days after inoculation, the diameter of the colonies was measured with calipers in millimeters (mm). The diameter of each colony was measured vertically once using the cross-sectional method, and the average value was taken.

[0031] 2.5 Data Statistics and Analysis 2.5.1 Calculation Method Based on the survey results, the inhibition rate of mycelial growth of the tested target bacteria by each treatment concentration was calculated according to formulas (1) and (2), and the unit is percentage (%).

[0032] D = D1 - D2 (1).

[0033] In the formula: D is the diameter of the colony growth; D1 is the diameter of the colony; D2 is the diameter of the mycelial cake.

[0034] I = (D0 - D) t )÷D0×100(2).

[0035] In the formula: I is the mycelial growth inhibition rate; D0 is the colony growth diameter of the blank control; D t Diameter of colonies grown after chemical treatment.

[0036] 2.5.2 Statistical Analysis Regression analysis was performed based on the logarithmic values ​​of each agent concentration and the corresponding mycelial growth inhibition probability values ​​to calculate the EC50 of each agent. 50 The co-toxicity coefficient (CTC) of the mixture was calculated according to Sun Yunpei's method to evaluate the combined effects of the mixture.

[0037] The specific calculation method is as follows: The calculation is performed using a single dose from the mixture as the standard reagent: Actual Toxicity Index (ATI) = (LC50 of Standard Reagent) 50 / LC of the tested drug efficacy 50 ) × 100; Theoretical toxicity index (TTI) = toxicity index of agent A × percentage content of A in the mixture + toxicity index of agent B × percentage content of B in the mixture; Co-toxicity coefficient (CTC) = [Actual toxicity index (ATI) of mixture / Theoretical toxicity index (TTI) of mixture] × 100; According to the NY / TI1547.7-2006 standard for classifying the combined effects of insecticides, a co-toxicity coefficient (CTC) ≥120 indicates a synergistic effect; a CTC ≤80 indicates an antagonistic effect; and 80 < CTC <120 indicates an additive effect.

[0038] Table 1. Combined virulence of boscalid and fludioxonil in inhibiting the mycelial growth of tomato gray mold.

[0039] 2.6 Results Analysis and Discussion Boscalid and fludioxonil have different chemical structures and mechanisms of action. Their combined use can help overcome or delay the development of fungal resistance and improve control efficacy. Experimental results show that different ratios of boscalid and fludioxonil exhibit synergistic effects in inhibiting the mycelial growth of tomato gray mold. When the ratio of boscalid to fludioxonil is 14:16–29:1, their co-toxicity coefficient (CTC) is ≥120, demonstrating excellent synergistic effects against tomato gray mold. Especially at ratios of 22:8–28:2, the activity is highest, with CTC exceeding 139, indicating a significant synergistic effect.

[0040] Example 1: 30% boscalid·fludioxonil suspension (22:8) The raw material components of 30% boscalid·fludioxonil suspension concentrate are as follows: 22g of cyazofamid technical grade, 8g of fludioxonil technical grade, 6g of dispersant (tristyrene-phenylphenol polyoxyethylene ether), 4.5g of wetting agent (potassium monododecyl phosphate), 3.5g of thickener (xanthan gum), 0.5g of organosilicon defoamer (modified polysiloxane), 5g of antifreeze (ethylene glycol), 2g of suspending agent (magnesium aluminum silicate), and deionized water to make up to 100g.

[0041] The preparation steps for 30% boscalid·fludioxonil suspension are as follows: First, 6g of dispersant (tristyrylphenol polyoxyethylene ether), 4.5g of wetting agent (potassium monododecyl phosphate), 3.5g of thickener (xanthan gum), 0.5g of organosilicon defoamer (modified polysiloxane), 5g of antifreeze (ethylene glycol), and 2g of suspending agent (magnesium aluminum silicate) were mixed. After high-speed shearing and dispersion, 22g of boscalid technical and 8g of fludioxonil technical were added, and zirconium grinding beads were added for ultrafine pulverization and grinding for 2 hours. Finally, deionized water was added for secondary mixing and stirring, and the mixture was filtered to obtain 30% boscalid·fludioxonil suspension.

[0042] Example 2: 30% boscalid·fludioxonil suspension (24:6) The raw material components of 30% boscalid·fludioxonil suspension concentrate are as follows: 24g of cyazofamid technical grade, 6g of fludioxonil technical grade, 8g of dispersant (polyoxyalkylarylphenyl ether phosphate salt), 3g of emulsifier (rosin polyoxyethylene ether), 5g of wetting agent (sodium dialkylsulfonyl succinate), 5g of thickener (xanthan gum), 1.5g of organosilicon defoamer (modified polysiloxane), 3g of antifreeze (ethylene glycol), 3g of suspending agent (magnesium aluminum silicate), and deionized water to make up to 100g.

[0043] The preparation steps for 30% boscalid·fludioxonil suspension are as follows: First, 8g of dispersant (polyoxyalkylarylphenyl ether phosphate salt), 3g of emulsifier (rosin polyoxyethylene ether), 5g of wetting agent (sodium dialkylsulfonyl succinate), 5g of thickener (xanthan gum), 1.5g of organosilicon defoamer (modified polysiloxane), 3g of antifreeze (ethylene glycol), and 3g of suspending agent (magnesium aluminum silicate) were mixed. After high-speed shearing and dispersion, 24g of boscalid technical and 6g of fludioxonil technical were added, and zirconium grinding beads were added for ultrafine pulverization and grinding for 2 hours. Finally, deionized water was added for secondary mixing and stirring, and the mixture was filtered to obtain 30% boscalid·fludioxonil suspension.

[0044] Example 3: 30% boscalid·fludioxonil suspension (28:2) The raw material components of 30% boscalid·fludioxonil suspension concentrate are as follows: 28g of cyazofamid technical grade, 2g of fludioxonil technical grade, 6g of dispersant (modified sulfonate anionic dispersant), 3g of emulsifier (tristyrene-based phenol polyoxyethylene ether), 4.5g of thickener (xanthan gum), 1.5g of silicone defoamer (modified polysiloxane), 5g of antifreeze (ethylene glycol), 1g of suspending agent (magnesium aluminum silicate), and deionized water to make up to 100g.

[0045] The preparation steps for 30% boscalid·fludioxonil suspension are as follows: First, 6g of dispersant (modified sulfonate anionic dispersant), 3g of emulsifier (tristyrene-based phenol polyoxyethylene ether), 4.5g of thickener (xanthan gum), 1.5g of organosilicon defoamer (modified polysiloxane), 5g of antifreeze (ethylene glycol), and 1g of suspending agent (magnesium aluminum silicate) were mixed. After high-speed shearing and dispersion, 28g of boscalid technical and 2g of fludioxonil technical were added, and zirconium grinding beads were added for ultrafine pulverization and grinding for 2 hours. Finally, deionized water was added for secondary mixing and stirring, and the mixture was filtered to obtain 30% boscalid·fludioxonil suspension.

[0046] Example 4: 30% boscalid·fludioxonil suspension (14:16) The raw material components of 30% boscalid·fludioxonil suspension concentrate are as follows: 14g of cyazofamid technical grade, 16g of fludioxonil technical grade, 7g of dispersant (rosin polyoxyethylene ether), 2.5g of wetting agent (sodium dialkyl sulfonyl succinate), 1.5g of thickener (xanthan gum), 0.6g of organosilicon defoamer (modified polysiloxane), 1.5g of antifreeze (ethylene glycol), 0.5g of preservative (Kason), 4g of suspending agent (magnesium aluminum silicate), and deionized water to make up to 100g.

[0047] The preparation steps for 30% boscalid·fludioxonil suspension are as follows: First, 7g of dispersant (rosin polyoxyethylene ether), 2.5g of wetting agent (sodium dialkyl sulfonyl succinate), 1.5g of thickener (xanthan gum), 0.6g of organosilicon defoamer (modified polysiloxane), 1.5g of antifreeze (ethylene glycol), 0.5g of preservative (Kason), and 4g of suspending agent (magnesium aluminum silicate) were mixed. After high-speed shearing and dispersion, 14g of boscalid technical and 16g of fludioxonil technical were added, and zirconium grinding beads were added for ultrafine pulverization and grinding for 2 hours. Finally, deionized water was added for secondary mixing and stirring, and the mixture was filtered to obtain 30% boscalid·fludioxonil suspension.

[0048] Example 5: 30% boscalid·fludioxonil suspension (29:1) The raw material components of 30% boscalid·fludioxonil suspension concentrate are as follows: 29g of cyazofamid technical grade, 1g of fludioxonil technical grade, 5g of dispersant (polyoxyalkylarylphenyl ether phosphate salt), 5.5g of emulsifier (tristyrene-based phenol polyoxyethylene ether), 2.5g of thickener (xanthan gum), 0.8g of silicone defoamer (modified polysiloxane), 3.5g of antifreeze (ethylene glycol), 0.5g of preservative (Kason), 2.5g of suspending agent (magnesium aluminum silicate), and deionized water to make up to 100g.

[0049] The preparation steps for 30% boscalid·fludioxonil suspension are as follows: First, 5g of dispersant (polyoxyalkylarylphenyl ether phosphate salt), 5.5g of emulsifier (tristyrene-based phenol polyoxyethylene ether), 2.5g of thickener (xanthan gum), 0.8g of organosilicon defoamer (modified polysiloxane), 3.5g of antifreeze (ethylene glycol), 0.5g of preservative (Kason), and 2.5g of suspending agent (magnesium aluminum silicate) were mixed. After high-speed shearing and dispersion, 29g of boscalid technical and 1g of fludioxonil technical were added, and zirconium grinding beads were added for ultrafine pulverization and grinding for 2 hours. Finally, deionized water was added for secondary mixing and stirring, and the mixture was filtered to obtain 30% boscalid·fludioxonil suspension.

[0050] Application Example 2: Field efficacy trial of 30% boscalid·fludioxonil suspension for controlling tomato gray mold 1. Experimental crop: Tomato, common tomato, autumn open-field cultivation, variety Gan Tomato No. 2; 2. Target groups for prevention and control: Tomato gray mold Botrylis cinerea Pers ; 3. Crop cultivation and environmental conditions The experiment was conducted at a vegetable base on a farm in the northern part of Nanchang City, Jiangxi Province. The soil type was mainly alluvial loam, with a pH of around 5.2. The soil topsoil was loose, with a high content of organic matter, and was deep and fertile. Tomatoes in the experimental field were sown and cultivated in early September, and transplanted into the open field in early October. They were planted in double rows with 1.2m wide beds and a plant spacing of approximately 33cm. At the time of the experiment, the tomatoes were in their vegetative growth stage, having developed 10-12 compound leaves. The experimental area had historically suffered severe damage from tomato gray mold, blight, and viral diseases. Other cultivation conditions in the experimental field were generally uniform.

[0051] 4. Test reagents and dosage Table 2 Test reagents and dosages

[0052] 5. Area of ​​the community and duplication Apartment size: 24m² 2 Number of repetitions: 4.

[0053] 6. Application method 6.1 Instructions for Use For spraying, the amount of pesticide solution to be sprayed is calculated based on 675 liters of pesticide solution per hectare, which is equivalent to 1.62 liters of pesticide solution per plot.

[0054] 6.2. Application equipment The Tianfengshou 3WBS-D-16 electric sprayer (product of Xinxiang Electric Sprayer Co., Ltd.) sprays pesticides at a working pressure of 0.15~0.25Mpa and a flow rate of 1.8~2.0 liters / minute.

[0055] 6.3 Application time and frequency The first application of pesticide was carried out on October 21st. The tomatoes were in the flowering and fruiting stage, and had grown 10-12 true leaves. There were sporadic cases of gray mold in the field. A second application was carried out 14 days later, on November 4th, for a total of two applications.

[0056] 7. Survey Methods 7.1 Survey Methods Before applying the pesticide, closely monitor the growth of tomatoes and the occurrence of tomato diseases. After applying the pesticide, observe the growth and appearance of tomatoes and the development of diseases from time to time. 14 days after the second application, on November 18, conduct a survey on the control effect.

[0057] Five sampling points were used for each plot, with two plants sampled at each point. All leaves and all fruits of each plant were investigated (if the number of fruits was too small, the number of plants sampled was increased appropriately). The total number of leaves (fruits), the number of diseased leaves (fruits), and the disease severity were recorded, and the control efficacy was calculated.

[0058] Leaf damage classification method (by leaf): Grade 0: No lesions; Grade 1: Three lesions on a single leaf; Grade 3: 4-6 lesions on a single leaf; Grade 5: 7-10 lesions on a single leaf; Grade 7: 11-20 lesions on a single leaf, some of which are densely clustered together; Grade 9: Disease spots on a single leaf covering more than a quarter of the leaf area.

[0059] Fruit damage grading method (by fruit): Grade 0: No lesions; Grade 1: Disease develops on residual petals or stigma; Grade 3: Sepals are rotten or the disease on the stigma has spread to the fruit navel; Grade 5: The fruit has a wetted spot at the navel but no mold layer; Grade 7: There is mold at the fruit's navel, but it has not spread to other parts; Level 9: The mold has spread to other parts of the fruit.

[0060] 7.2 Method for Calculating Prevention Efficacy ; 7.3 Test Results Table 3 Experimental Results

[0061] The experimental results showed that the tested agent, 30% cyazofamid·fludioxonil suspension, had a good control effect on tomato gray mold. The dosage is 450-900 grams per hectare, which is equivalent to 135-270 grams of active ingredient diluted in 675 liters of water. Spray at the early stage of tomato gray mold occurrence. Apply a second application 14 days after the first application. The efficacy was investigated 14 days after the second application. The efficacy against leaves reached 81.38%-87.30%, and the efficacy against tomato fruits reached 83.73%-90.06%. The efficacy of the highest dosage treatment was significantly higher than that of the control agent 30% cyprodinil suspension at a dosage of 750 grams per hectare (equivalent to 225 grams of active ingredient) (79.90%, 82.66%) and the control agent 50% fludioxonil wettable powder at a dosage of 108 grams per hectare (equivalent to 54 grams of active ingredient) (80.69%, 83.39%). However, the efficacy of the lowest dosage treatment was basically the same as that of the two control agents, and no significant difference was found. The experimental agent is safe for tomato growth and has a certain effect on controlling early and late blight in tomatoes. This agent is worth promoting and applying in production.

[0062] This invention contains a synergistic composition of boscalid and fludioxonil, which can be prepared into any formulation suitable for agricultural use using known methods. This invention has screened and selected a preferred suspension concentrate through experiments, and boscalid and fludioxonil have a significant synergistic effect on tomato gray mold when the ratio is 14:16 to 29:1. In particular, the control effect on tomato gray mold is best when the ratio of boscalid and fludioxonil is 22:8 to 28:2, and the synergistic effect is significant.

Claims

1. A bactericidal composition containing boscalid and fludioxonil, characterized in that: The bactericidal composition contains the active ingredients of cyazofamid and fludioxonil.

2. The bactericidal composition containing cyazofamid and fludioxonil according to claim 1, characterized in that: The mass ratio of cyazofamid to fludioxonil is 10:20 to 29:

1.

3. The bactericidal composition containing cyazofamid and fludioxonil according to claim 2, characterized in that: The mass ratio of cyazofamid to fludioxonil is 14:16 to 29:

1.

4. The bactericidal composition containing cyazofamid and fludioxonil according to claim 3, characterized in that: The mass ratio of cyazofamid to fludioxonil is 22:8 to 28:

2.

5. The bactericidal composition containing cyazofamid and fludioxonil according to claim 1, characterized in that: The sum of the masses of cyazofamid and fludioxonil in the bactericidal composition is 1 to 60% of the total mass of the bactericidal composition.

6. The bactericidal composition containing cyazofamid and fludioxonil according to claim 5, characterized in that: The sum of the masses of cyazofamid and fludioxonil in the bactericidal composition is 1% to 30% of the total mass of the bactericidal composition.

7. The bactericidal composition containing boscalid and fludioxonil according to claim 1, characterized in that: The bactericidal composition also includes adjuvants and / or fillers that are permitted and acceptable in agronomy.

8. The bactericidal composition containing cyazofamid and fludioxonil according to claim 7, characterized in that: The adjuvants permitted and acceptable in the pesticide science are penetrants, dispersants, diffusion agents, emulsifiers, stabilizers, wetting agents, thickeners, defoamers, antifreeze agents, or synergists; the filler is water.

9. The bactericidal composition containing cyazofamid and fludioxonil according to any one of claims 1-8, characterized in that: The bactericidal composition is prepared into an agriculturally permissible formulation; the formulation is a suspension, microcapsule suspension, wettable powder, emulsifiable concentrate, microemulsion, soluble concentrate, water emulsion, water-dispersible granules, suspension emulsion, or ultra-low volume liquid.

10. The application of the fungicidal composition containing cyazofamid and fludioxonil according to claim 9 in the prevention and control of plant diseases, characterized in that: The plant disease mentioned is tomato gray mold.

Images